CA1197189A - Procedures for preparing composite materials from collagen and glycosaminoglycan - Google Patents

Abstract

PROCEDURES FOR PREPARING
COMPOSITE MATERIALS FROM
COLLAGEN AND GLYCOSAMINOGLYCAN

Abstract This invention relates to crosslinked collagen and glycosaminoglycan materials, and to procedures for preparing such materials. It has been discovered that if collagen fibrils in an aqueous acidic solution are contacted with a crosslinking agent before being contacted with glycosaminoglycan, the materials produced have extremely low levels of thrombogenicity.
Such materials are well suited for in-dwelling catheters, blood vessel grafts, and other devices that are in continuous contact with blood for long periods of time.

Description

~7~L~19 1 Descrip't'ion Government Suppo:rt The invention described herein was supported in whole or in part by a grant from the National Institutes of Health.
Technica'l Field This invention is in the fields o~ polymeric materials and biocompatible materials.
' Back`ground Art Composite materials made of collagen and glycosaminoglycan (GAG) have been shown to be highly useful for certain biochemical application~. For example, U.S. Patent 4,060,081 (Yannas et al, 1977) r discloses a multilayer membrane suitable for use as synthetic skin.
The bottom layer, which is placed in contac-t ~ith'a woundbed, is a highly porous lattice comprising collagen that i5 crosslinked with GAG This lattice provides a biophysical supporting structure in which cells can migrate and proliferate to heal the wound.
The typical procedures that have been used in the past to prepare collagen/GAG composite materials are described in U.S. Patent 4,280,954 (Yannas et al, 1981).
Briefly, a preferred embodiment of those procedures com-prises the following steps, in se~uence:

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-2- ( 1 1. Mechanically cutting and grinding a source of collagen into particulate form.
2. Soaking the particulate collagen in dilute acetic acid.

3. Homogenizing the solution in a blender.

4. Adding a source of glycosaminoglycan which has been ground into particulate form. Typically, enough GAG is added to the solution to comprise about 6~ to about 12% by dry weight of the composite material. The collagen/GAG mixture normally precipitates out of the acidic solution and forms a fibrous dispersion.

5. The precipitate is homogenized in a blender.

6. The solution is contacted with a crosslinking agent such as glutaraldehyde.

7. The solution is quickly frozen in a shallow pan.

8. The frozen dispersion is subjected to a high degree of vacuum, causing the acidic fluid to evaporate while the spatial conflguration of the partially crosslinked fibrils is maintained.

The composite material thus formed may be treated by additional procedures to remove all traces of glutaraldehyde and to increase the crosslinking density and strength of the composite mate~ial.
The resulting collagen/GAG composite material exhibits some degree of incompatibility with the blood. ~or example, a small amount of platelet aggregation may occur over a period of several months if blood is in con-tinuous contact with this material.
This degree of blood incompatibility might be tolerable if the collagen/GAG composite material is -3- ~L~

1 to be biodegraded over a relatively short period of time, as is the case when it is used as artificial skin. However, that level of blood incompatibility can lead to certain problems in the use of collagen~GAG
composite materials for prosthetic devices which are not rapidly biodegraded and which are in continous contact with blood. For example, collagen/GAG
material, in a less porous form than the lattice used for synthetic skin, may be useful for in-dwelling catheters, blood vessel replacement material, and other prosthetic devices. For such uses, it is advantageous to utili~e a composite material that does not cause platelet aggregation to any detectable extent.

. _ _4_ 1 Disclosure of the Invention This invention relates to a method of creating collagen and glycosaminoglycan (GAG) composite materials that have extremely high levels of blood compatibility. The Applicants have discovered that if an aqueous dispersion of collagen which has been swollen in acid is contacted with a crosslinking agent such as glutaraldehyde prior to adding GAG to the solution, the resulting composite material will cause extremely low or undetectable levels of blood platelet aggregation. The invention described herein also relates to composite materials formed by this method. These materials are highly useful for prosthetic devices that will remain in contact with blood for very long periods of time, including indwelling catheters, blood vessel grafts, and other prosthetic devices that are not rapidly biodegraded.

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1 sest Mode For Carrying Out This Invention In one preferred embodiment of this invention, a source of collagen is ground into pa ticulate form and contacted with dilute acetic acid. The acidic solution is stirred to ensure thorough contact of all collagen with acid~ The collagen is subsequently allowed to swell for 72 hours to remove all na~ive banding.
After swelling, a crosslinkiny agent comprising glutaraldehyde is added in the form of an aqueous solution. The final glutaraldehyde concentration is approximately equal to 0.5% weight/volume. The solution normally is stirred while the glutaraldehyde is being added.
Following the addition of glutaraldehyde, a solution of chondroitin-6-sulfate (which is a glycosaminoglycan) is added to the acidic collagen solution. The chondroitin 6-sulfate is first dissolved in dilute acetic acid, and added drop-wise to the collagen solution while it is beingstirred.
The resulting dispersion may be treated in any of several ways. In order to create a prosthetic device, it may be frozen and lyophilized. In order to test its blood compatibility propertiesl it may be filtered, air-dried, ground into particulate form, and dispersed in physiological saline solution.

7~J9 Example 1: Preparation of Collagen/GAG Material Collagen from bovine hide, prepared by the methods described by M. ~omanowsky et al, J. Amer.
Leather Chemists Assn. 69: No.9, p. 410-422 (1974), was donated b~ H. ~. Sinnamon of the V.S. Dept. of Agriculture. 0.55 g. of freeze-dried collagen was ground in a Wiley mill (A. H. Thomas Co., Phila. PA) to a 60 mesh particle si~e. It was added to 200 ml of 0.05 M aqueous acetic acid. This solution was stirred for 60 minutes in an ice-jacketed blender (Eberbach Corp., Ann Arbor, MI) on a 2-speed power unit (Waring Co., Hartford, CT) set on high speed with the line voltage reduced to 50% of 120 volts.
Following this blending step, the solution was placed in a tightly closed glass jar and the collagen was allowed to swell for 72 hours at 4C.
A solution of 25% glutaraldehyde ~'Baker-analyzed"
reagent grade, J. T. Baker Co., Phila. PA) i~
distilled water was prepared. A sufficient quantity of this solution was added to the acidic collagen - solution to comprise 0.5% glutaraldehyde yolume per volume. The glutaraldehyde solution was added while the dispersion was blended for one hour in an over-head blender (Hamilton Beach Div. of Scovill, Washington, ~.C.) set on the low speed with the line voltage reduced to 60% of 120 volts.
0.0578 g chondroitin 6-sulfate was dissolved in 10 ml of 0.05 M acetic acid. This solution was added to 175 ml of the glutaraldehyde-treated collagen dispersion. The addition was performed over a period of 5 minutes while the dispersion was being blended in the overhead blendern Shortly thereafter, the dispersion was filtered in a Buechner funnel. This filtering step was ( _7~ >7~

l completed in about 20 minutes. The resulting wet membrane was then air-dried and milled to a 60 mesh particle size. It was dispersed in physiological saline solution (0.15 M NaCl, pH7) priox to being contacted with blood platelets.

Example 2: Preparation of Collagen/GAG Control Materials Two other dispersions o~ collagen/GAG were pre-pared as described above, with the following changes.
In one specimen, the glutaraldehyde crosslinXing lO step was omitted. In the other specimen, chondroitin 6-sulfate was added to the collagen dispersion prior to the addition of glutaraldehyde.

Example 3: Blood Compatibility Testing Blood plasma that was rich in platelet content was obtained from healthy, drug-free human donors (Beth Israel Hospital, Boston, MA) by using the procedures described in F. H. Silver et al, "Glycosaminoglycan Inhibition of Collagen-Induced Platelet Aggregation," Thrombosis Research 13:
20 267-277 (1978). Platelet clotting was measured ; with an aggregometer (Chrono-Log Corporation Model 300, Broomall, PA).
When platelet-rich plasma was contacted with the collagen/GAG dispersion that had not been contacted with glutaraldehyde, the collagen/GAG
material caused rapid formation of a platelet clot. When the platelet-rich plasma was contacted with the collagen/GAG that had been contacted with glutaraldehyde subsequent to the addition of chondroitin 6-sulfate, a small amount of platelet aggregation occurred if the dispersion had been stored for a period of several months in --8~
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1 physiological saline prior to being contacted with platelet-rich plasma. By contrast, when the glutaraldehyde crosslinking step preceded the addition of chondroitin 6-sulfate, as described in -Example 1, no platelet aggregation could be detected, regardless of whether the dispersion was stored in physiological saline for up to one month prior to being contacted with platelet-rich plasma~

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1 Industrial ~pplicability This invention has industrial applicability in the preparation of collagen prosthetic devices that have very high levels of blood compatibility.
Equivalents Those skilled in the art will recognize, or be able to ascertain using no more than routine experimentation, numerous equivalents to the specific procedures described herein. Such equivalents are considered to be within the scope of this invention, and are covered by the following claims.

Claims (6)

1. A method for preparing a crosslinked collagen-glycosaminoglycan composite material, comprising:
a. soaking collagen in an aqueous acidic solution having a pH of below about six;
b. contacting said solution with a cross-linking agent; and c. contacting a source of glycosaminoglycan with said solution.

2. In the method of preparing crosslinked collagen-glycosaminoglycan composite material, the improvement of contacting collagen, which has been soaked in an aqueous acidic solution, with a crosslinking agent prior to contacting said collagen and said crosslinking agent with glycosaminoglycan.

3. A method of Claims 1 or 2 wherein said aqueous acidic solution comprises acetic acid.

4. A method of Claims 1 or 2 wherein said crosslinking agent comprises glutaraldehyde.

5. A method of Claims 1 or 2 wherein said glycosaminoglycan is selected from the following group: chondroitin 6-sulfate, chondroitin 4-sulfate, heparin, heparin sulfate, keratan sulfate, dermatan sulfate, or chitin.

6. A crosslinked collagen-lycosaminoglycan composite material made by the methods of Claims 1 or 2.