CA2060505A1 - Method for recovering recombinant proteins - Google Patents
Method for recovering recombinant proteinsInfo
- Publication number
- CA2060505A1 CA2060505A1 CA002060505A CA2060505A CA2060505A1 CA 2060505 A1 CA2060505 A1 CA 2060505A1 CA 002060505 A CA002060505 A CA 002060505A CA 2060505 A CA2060505 A CA 2060505A CA 2060505 A1 CA2060505 A1 CA 2060505A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- molecular weight
- high molecular
- solution
- further processed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormones [GH] (Somatotropin)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/303—Extraction; Separation; Purification by precipitation by salting out
Abstract
A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding amine or quaternary ammonium compounds to the solution in amounts sufficient to selectively pre-cipitate the high molecular weight protein contaminants. The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/364,846 US5101018A (en) | 1989-06-12 | 1989-06-12 | Method for recovering recombinant proteins |
US364,846 | 1989-06-12 | ||
PCT/US1990/002652 WO1990015876A1 (en) | 1989-06-12 | 1990-05-10 | Method for recovering recombinant proteins |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2060505A1 true CA2060505A1 (en) | 1990-12-13 |
CA2060505C CA2060505C (en) | 2001-06-12 |
Family
ID=23436347
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002060505A Expired - Fee Related CA2060505C (en) | 1989-06-12 | 1990-05-10 | Method for recovering recombinant proteins |
Country Status (14)
Country | Link |
---|---|
US (1) | US5101018A (en) |
EP (1) | EP0477198B1 (en) |
JP (1) | JP2957695B2 (en) |
KR (1) | KR0178274B1 (en) |
AT (1) | ATE96805T1 (en) |
AU (1) | AU634547B2 (en) |
BR (1) | BR9007433A (en) |
CA (1) | CA2060505C (en) |
DE (1) | DE69004429T2 (en) |
DK (1) | DK0477198T3 (en) |
ES (1) | ES2046782T3 (en) |
HU (2) | HU209651B (en) |
RU (1) | RU2075509C1 (en) |
WO (1) | WO1990015876A1 (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5047511A (en) * | 1989-08-28 | 1991-09-10 | Pitman-Moore, Inc. | Method for recovering recombinant proteins |
US5605691A (en) * | 1992-09-17 | 1997-02-25 | Ophidian Pharmaceuticals, Inc. | Immunologically active proteins from inclusion bodies |
US5869604A (en) * | 1995-11-09 | 1999-02-09 | Georgia Institute Of Technology | Crystallization and purification of polypeptides |
CA2498319A1 (en) * | 2002-09-09 | 2004-03-18 | Nautilus Biotech | Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules |
US7998930B2 (en) | 2004-11-04 | 2011-08-16 | Hanall Biopharma Co., Ltd. | Modified growth hormones |
TWI504405B (en) * | 2009-01-22 | 2015-10-21 | Novo Nordisk Healthcare Ag | Stable growth hormone compounds |
WO2011089255A1 (en) | 2010-01-22 | 2011-07-28 | Novo Nordisk Health Care Ag | Growth hormones with prolonged in-vivo efficacy |
EP2525833A2 (en) | 2010-01-22 | 2012-11-28 | Novo Nordisk Health Care AG | Stable growth hormone compounds |
ES2841123T3 (en) | 2013-04-05 | 2021-07-07 | Formulacion Del Compuesto De La Hormona De Crecimiento | Growth hormone compound formulation |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2175090A (en) * | 1938-06-22 | 1939-10-03 | Lederie Lab Inc | Purification of antibody compositions |
US3657071A (en) * | 1968-11-12 | 1972-04-18 | Hans Ulrich Bergmeyer | Fractionation of proteins |
US3749668A (en) * | 1971-05-03 | 1973-07-31 | Rohm & Haas | Water purification with amine oxide resins |
DE2718325C2 (en) * | 1977-04-25 | 1986-09-25 | Behringwerke Ag, 3550 Marburg | High Leucine 3.1-S-αγ2γ-glycoprotein and its use |
US4204989A (en) * | 1978-12-13 | 1980-05-27 | Merck & Co., Inc. | Isolation of hepatitis B e antigen |
DE2946458A1 (en) * | 1979-11-17 | 1981-06-11 | Behringwerke Ag, 3550 Marburg | NEW PROTEIN PP (DOWN ARROW) 11 (DOWN ARROW) |
US4778813A (en) * | 1981-07-07 | 1988-10-18 | Buckman Laboratories International, Inc. | Polymeric quaternary ammonium compounds, their preparation and use |
JPH0738880B2 (en) * | 1983-06-24 | 1995-05-01 | 東レ株式会社 | Endotoxemia treatment |
US4634673A (en) * | 1984-03-28 | 1987-01-06 | Nabisco Brands, Inc. | Enzyme purification process |
DE3484713D1 (en) * | 1984-09-10 | 1991-07-18 | Toray Industries | ION EXCHANGE OR ADSORPTION PROCEDURE. |
SE462100B (en) * | 1985-08-08 | 1990-05-07 | Perstorp Ab | COMPOSITION AND ITS USE IN A TWO OR MULTIPLE SYSTEM |
US4677196A (en) * | 1985-09-06 | 1987-06-30 | International Minerals & Chemical Corp. | Purification and activation of proteins from insoluble inclusion bodies |
DE3608091A1 (en) * | 1986-03-12 | 1987-09-17 | Basf Ag | METHOD FOR INSULATING AND CLEANING HAEMIN |
US4771128A (en) * | 1986-10-10 | 1988-09-13 | Cetus Corporation | Method of purifying toxin conjugates using hydrophobic interaction chromatography |
AU612133B2 (en) * | 1987-02-20 | 1991-07-04 | Natinco Nv | Production of proteins in active forms |
EP0295859B1 (en) * | 1987-06-15 | 1994-11-17 | Southern Cross Biotech Pty.Ltd. | Production of proteins in active forms |
-
1989
- 1989-06-12 US US07/364,846 patent/US5101018A/en not_active Expired - Lifetime
-
1990
- 1990-05-10 HU HU904388A patent/HU209651B/en not_active IP Right Cessation
- 1990-05-10 DK DK90907861.0T patent/DK0477198T3/en active
- 1990-05-10 AT AT90907861T patent/ATE96805T1/en not_active IP Right Cessation
- 1990-05-10 RU SU905010961A patent/RU2075509C1/en active
- 1990-05-10 DE DE90907861T patent/DE69004429T2/en not_active Expired - Fee Related
- 1990-05-10 CA CA002060505A patent/CA2060505C/en not_active Expired - Fee Related
- 1990-05-10 AU AU56442/90A patent/AU634547B2/en not_active Ceased
- 1990-05-10 WO PCT/US1990/002652 patent/WO1990015876A1/en active IP Right Grant
- 1990-05-10 ES ES199090907861T patent/ES2046782T3/en not_active Expired - Lifetime
- 1990-05-10 BR BR909007433A patent/BR9007433A/en not_active Application Discontinuation
- 1990-05-10 HU HU8843Q patent/HUT61053A/en unknown
- 1990-05-10 EP EP90907861A patent/EP0477198B1/en not_active Expired - Lifetime
- 1990-05-10 JP JP2507613A patent/JP2957695B2/en not_active Expired - Fee Related
- 1990-05-10 KR KR1019910701832A patent/KR0178274B1/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ATE96805T1 (en) | 1993-11-15 |
DE69004429D1 (en) | 1993-12-09 |
HU209651B (en) | 1994-09-28 |
JPH04505706A (en) | 1992-10-08 |
AU634547B2 (en) | 1993-02-25 |
AU5644290A (en) | 1991-01-08 |
HU904388D0 (en) | 1992-04-28 |
EP0477198B1 (en) | 1993-11-03 |
DE69004429T2 (en) | 1994-03-31 |
WO1990015876A1 (en) | 1990-12-27 |
JP2957695B2 (en) | 1999-10-06 |
DK0477198T3 (en) | 1993-11-29 |
KR0178274B1 (en) | 1999-04-01 |
BR9007433A (en) | 1992-06-16 |
US5101018A (en) | 1992-03-31 |
HUT61053A (en) | 1992-11-30 |
CA2060505C (en) | 2001-06-12 |
RU2075509C1 (en) | 1997-03-20 |
ES2046782T3 (en) | 1994-02-01 |
EP0477198A1 (en) | 1992-04-01 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed | ||
MKLA | Lapsed |
Effective date: 20040510 |