CA2092115A1 - Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) - Google Patents

Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Info

Publication number
CA2092115A1
CA2092115A1 CA2092115A CA2092115A CA2092115A1 CA 2092115 A1 CA2092115 A1 CA 2092115A1 CA 2092115 A CA2092115 A CA 2092115A CA 2092115 A CA2092115 A CA 2092115A CA 2092115 A1 CA2092115 A1 CA 2092115A1
Authority
CA
Canada
Prior art keywords
maculans
dna
infestation
testing
pcr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2092115A
Other languages
French (fr)
Other versions
CA2092115C (en
Inventor
Janet L. Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Council of Canada
Original Assignee
Janet L. Taylor
National Research Council Of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janet L. Taylor, National Research Council Of Canada filed Critical Janet L. Taylor
Priority to CA002092115A priority Critical patent/CA2092115C/en
Priority to EP94908903A priority patent/EP0689591B1/en
Priority to PCT/CA1994/000130 priority patent/WO1994021788A2/en
Priority to US08/521,053 priority patent/US5916744A/en
Publication of CA2092115A1 publication Critical patent/CA2092115A1/en
Application granted granted Critical
Publication of CA2092115C publication Critical patent/CA2092115C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00Chemistry: molecular biology and microbiology
    • Y10S435/81Packaged device or kit

Abstract

A method of testing for infestation of tissue of rape or other Cruciferae with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
CA002092115A 1993-03-22 1993-03-22 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) Expired - Fee Related CA2092115C (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002092115A CA2092115C (en) 1993-03-22 1993-03-22 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)
EP94908903A EP0689591B1 (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)
PCT/CA1994/000130 WO1994021788A2 (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)
US08/521,053 US5916744A (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus Leptosphaeria maculans (blackleg infestation)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002092115A CA2092115C (en) 1993-03-22 1993-03-22 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Publications (2)

Publication Number Publication Date
CA2092115A1 true CA2092115A1 (en) 1994-09-23
CA2092115C CA2092115C (en) 1998-12-15

Family

ID=4151334

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002092115A Expired - Fee Related CA2092115C (en) 1993-03-22 1993-03-22 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Country Status (4)

Country Link
US (1) US5916744A (en)
EP (1) EP0689591B1 (en)
CA (1) CA2092115C (en)
WO (1) WO1994021788A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
ES2269102T3 (en) 1999-02-25 2007-04-01 Exact Sciences Corporation PROCEDURES TO PRESERVE THE INTEGRITY OF DNA.
ATE331811T1 (en) 1999-04-09 2006-07-15 Exact Sciences Corp METHOD FOR DETECTING NUCLEIC ACIDS INdicative of CANCER
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
US7368233B2 (en) * 1999-12-07 2008-05-06 Exact Sciences Corporation Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm
AU2002232118A1 (en) * 2002-02-11 2003-09-04 Secretary, Department Of Atomic Energy A method of pcr based detection and identification of rice blast fungus magnaporthe grisea
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
US7462448B2 (en) 2002-08-02 2008-12-09 Stratatech Corporation Species specific DNA detection
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
US20110219466A1 (en) * 2007-10-05 2011-09-08 Dilantha Fernando Sequence-characterized amplified region (scar) markers for plant resistance against fungal pathogens
US20110148155A1 (en) * 2009-12-22 2011-06-23 Mattel, Inc. Collapsible Infant Support
CN101805794B (en) * 2010-04-08 2012-05-02 中华人民共和国上海出入境检验检疫局 Real-time fluorescent PCR detection method of L.maculans as well as primer and probe used for detection
CN101805793B (en) * 2010-04-08 2012-04-18 中华人民共和国上海出入境检验检疫局 PCR detection method of L.maculans and primer used for detection
CN103255207A (en) * 2013-01-15 2013-08-21 内蒙古农牧业科学院 Molecular detection method for brassica campestris seeds carrying brassica campestris leptosphaeria maculans pathogenic bacterial
CN103131778A (en) * 2013-02-05 2013-06-05 内蒙古农牧业科学院 Molecular detection method of rapeseed-carried rapeseed blackleg pathogen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4127862A1 (en) * 1991-08-21 1993-02-25 Inst Genbiologische Forschung Aggressive and non-aggressive pathotype distinction of phoma lingam - using random primers of 8-11 nucleotide(s) giving differing pattern after gel-electrophoresis, useful in plant protection

Also Published As

Publication number Publication date
US5916744A (en) 1999-06-29
EP0689591A1 (en) 1996-01-03
WO1994021788A2 (en) 1994-09-29
EP0689591B1 (en) 1997-08-06
WO1994021788A3 (en) 1994-12-22
CA2092115C (en) 1998-12-15

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