CA2092115A1 - Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) - Google Patents
Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)Info
- Publication number
- CA2092115A1 CA2092115A1 CA2092115A CA2092115A CA2092115A1 CA 2092115 A1 CA2092115 A1 CA 2092115A1 CA 2092115 A CA2092115 A CA 2092115A CA 2092115 A CA2092115 A CA 2092115A CA 2092115 A1 CA2092115 A1 CA 2092115A1
- Authority
- CA
- Canada
- Prior art keywords
- maculans
- dna
- infestation
- testing
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000228457 Leptosphaeria maculans Species 0.000 title abstract 8
- 206010061217 Infestation Diseases 0.000 title abstract 3
- 241000219193 Brassicaceae Species 0.000 title abstract 2
- 244000188595 Brassica sinapistrum Species 0.000 title 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 title 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000010998 test method Methods 0.000 abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 101001038337 Homo sapiens Serine/threonine-protein kinase LMTK1 Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 102100040293 Serine/threonine-protein kinase LMTK1 Human genes 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 238000002405 diagnostic procedure Methods 0.000 abstract 1
- 230000003252 repetitive effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Abstract
A method of testing for infestation of tissue of rape or other Cruciferae with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002092115A CA2092115C (en) | 1993-03-22 | 1993-03-22 | Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) |
EP94908903A EP0689591B1 (en) | 1993-03-22 | 1994-03-14 | Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) |
PCT/CA1994/000130 WO1994021788A2 (en) | 1993-03-22 | 1994-03-14 | Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) |
US08/521,053 US5916744A (en) | 1993-03-22 | 1994-03-14 | Testing for infestation of rapeseed and other cruciferae by the fungus Leptosphaeria maculans (blackleg infestation) |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002092115A CA2092115C (en) | 1993-03-22 | 1993-03-22 | Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2092115A1 true CA2092115A1 (en) | 1994-09-23 |
CA2092115C CA2092115C (en) | 1998-12-15 |
Family
ID=4151334
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002092115A Expired - Fee Related CA2092115C (en) | 1993-03-22 | 1993-03-22 | Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) |
Country Status (4)
Country | Link |
---|---|
US (1) | US5916744A (en) |
EP (1) | EP0689591B1 (en) |
CA (1) | CA2092115C (en) |
WO (1) | WO1994021788A2 (en) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6406857B1 (en) | 1997-06-16 | 2002-06-18 | Exact Sciences Corporation | Methods for stool sample preparation |
ES2269102T3 (en) | 1999-02-25 | 2007-04-01 | Exact Sciences Corporation | PROCEDURES TO PRESERVE THE INTEGRITY OF DNA. |
ATE331811T1 (en) | 1999-04-09 | 2006-07-15 | Exact Sciences Corp | METHOD FOR DETECTING NUCLEIC ACIDS INdicative of CANCER |
US6586177B1 (en) * | 1999-09-08 | 2003-07-01 | Exact Sciences Corporation | Methods for disease detection |
US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
US6919174B1 (en) * | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
US7368233B2 (en) * | 1999-12-07 | 2008-05-06 | Exact Sciences Corporation | Methods of screening for lung neoplasm based on stool samples containing a nucleic acid marker indicative of a neoplasm |
AU2002232118A1 (en) * | 2002-02-11 | 2003-09-04 | Secretary, Department Of Atomic Energy | A method of pcr based detection and identification of rice blast fungus magnaporthe grisea |
US7776524B2 (en) | 2002-02-15 | 2010-08-17 | Genzyme Corporation | Methods for analysis of molecular events |
US7462448B2 (en) | 2002-08-02 | 2008-12-09 | Stratatech Corporation | Species specific DNA detection |
US20040259101A1 (en) * | 2003-06-20 | 2004-12-23 | Shuber Anthony P. | Methods for disease screening |
WO2005113769A1 (en) * | 2004-05-14 | 2005-12-01 | Exact Sciences Corporation | Method for stabilizing biological samples for nucleic acid analysis |
US20110219466A1 (en) * | 2007-10-05 | 2011-09-08 | Dilantha Fernando | Sequence-characterized amplified region (scar) markers for plant resistance against fungal pathogens |
US20110148155A1 (en) * | 2009-12-22 | 2011-06-23 | Mattel, Inc. | Collapsible Infant Support |
CN101805794B (en) * | 2010-04-08 | 2012-05-02 | 中华人民共和国上海出入境检验检疫局 | Real-time fluorescent PCR detection method of L.maculans as well as primer and probe used for detection |
CN101805793B (en) * | 2010-04-08 | 2012-04-18 | 中华人民共和国上海出入境检验检疫局 | PCR detection method of L.maculans and primer used for detection |
CN103255207A (en) * | 2013-01-15 | 2013-08-21 | 内蒙古农牧业科学院 | Molecular detection method for brassica campestris seeds carrying brassica campestris leptosphaeria maculans pathogenic bacterial |
CN103131778A (en) * | 2013-02-05 | 2013-06-05 | 内蒙古农牧业科学院 | Molecular detection method of rapeseed-carried rapeseed blackleg pathogen |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4127862A1 (en) * | 1991-08-21 | 1993-02-25 | Inst Genbiologische Forschung | Aggressive and non-aggressive pathotype distinction of phoma lingam - using random primers of 8-11 nucleotide(s) giving differing pattern after gel-electrophoresis, useful in plant protection |
-
1993
- 1993-03-22 CA CA002092115A patent/CA2092115C/en not_active Expired - Fee Related
-
1994
- 1994-03-14 WO PCT/CA1994/000130 patent/WO1994021788A2/en active IP Right Grant
- 1994-03-14 US US08/521,053 patent/US5916744A/en not_active Expired - Fee Related
- 1994-03-14 EP EP94908903A patent/EP0689591B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US5916744A (en) | 1999-06-29 |
EP0689591A1 (en) | 1996-01-03 |
WO1994021788A2 (en) | 1994-09-29 |
EP0689591B1 (en) | 1997-08-06 |
WO1994021788A3 (en) | 1994-12-22 |
CA2092115C (en) | 1998-12-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request | ||
MKLA | Lapsed |