CA2154013A1 - Analytical element, composition and method using modified apo-horseradish peroxidase - Google Patents

Analytical element, composition and method using modified apo-horseradish peroxidase

Info

Publication number
CA2154013A1
CA2154013A1 CA002154013A CA2154013A CA2154013A1 CA 2154013 A1 CA2154013 A1 CA 2154013A1 CA 002154013 A CA002154013 A CA 002154013A CA 2154013 A CA2154013 A CA 2154013A CA 2154013 A1 CA2154013 A1 CA 2154013A1
Authority
CA
Canada
Prior art keywords
apo
hemoglobin
composition
horseradish peroxidase
analytical element
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002154013A
Other languages
French (fr)
Other versions
CA2154013C (en
Inventor
Roy Eugene Snoke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ortho Clinical Diagnostics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2154013A1 publication Critical patent/CA2154013A1/en
Application granted granted Critical
Publication of CA2154013C publication Critical patent/CA2154013C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Abstract

Modified apo-peroxidases have been found useful in analytical methods to remove the bias caused by the presence of hemoglobin in the test specimens.
While apo-peroxidases are known to remove serum interferents, they fail to overcome the hemoglobin bias, particularly when dry coated analytical elements are used in the assays. Blocking the nitrogen atoms of the imidzolyl groups of the histidine amino acids of the apo-peroxidase prevents catalytic activity of the protein in the presence of hemoglobin.
CA002154013A 1994-07-19 1995-07-17 Analytical element, composition and method using modified apo-horseradish peroxidase Expired - Lifetime CA2154013C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27739194A 1994-07-19 1994-07-19
US08/277,391 1994-07-19

Publications (2)

Publication Number Publication Date
CA2154013A1 true CA2154013A1 (en) 1996-01-20
CA2154013C CA2154013C (en) 2006-12-19

Family

ID=23060663

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002154013A Expired - Lifetime CA2154013C (en) 1994-07-19 1995-07-17 Analytical element, composition and method using modified apo-horseradish peroxidase

Country Status (7)

Country Link
US (1) US5776714A (en)
EP (1) EP0693552B1 (en)
JP (1) JP3715348B2 (en)
AT (1) ATE236246T1 (en)
AU (1) AU697785B2 (en)
CA (1) CA2154013C (en)
DE (1) DE69530148T2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19628484A1 (en) * 1996-07-15 1998-01-22 Boehringer Mannheim Gmbh Blood substitute suppression by peroxides
CA2270797A1 (en) * 1998-07-27 2000-01-27 Bayer Corporation Transparent flow through membrane for dry reagent analytical devices
AU2001278034A1 (en) * 2000-07-26 2002-02-05 Bio-Diagnostics Inc. Method for diagnosing thyroid conditions and for monitoring thyroxine therapy
US7348147B2 (en) * 2004-11-15 2008-03-25 Carestream Health, Inc. Method and system for nucleic acid detection
US7074622B2 (en) * 2004-11-15 2006-07-11 Eastman Kodak Company Method and system for sorting and separating particles
US7183119B2 (en) * 2004-11-15 2007-02-27 Eastman Kodak Company Method for sensitive detection of multiple biological analytes
US20090282902A1 (en) * 2006-08-17 2009-11-19 Peter Warthoe Bio surface acoustic wave (saw) resonator amplification for detection of a target analyte
EP1949894B1 (en) * 2007-01-25 2016-06-29 Roche Diagnostics GmbH Enhancement of vanadium-containing phosphatase inhibitors by polyols
US8008035B2 (en) * 2007-01-25 2011-08-30 Roche Diagnostics Operations, Inc. Enhancement of vanadium-containing phosphatase inhibitors
CN111518780B (en) * 2020-06-04 2022-06-14 郑州伊美诺生物技术有限公司 Preparation method of apo-horseradish peroxidase

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3992158A (en) 1973-08-16 1976-11-16 Eastman Kodak Company Integral analytical element
US3905872A (en) 1973-12-14 1975-09-16 American Cyanamid Co Alkaline phosphatase test material
US4089747A (en) 1976-08-09 1978-05-16 Eastman Kodak Company Compositions for the detection of hydrogen peroxide
US4376165A (en) * 1978-06-22 1983-03-08 Miles Laboratories, Inc. Method of preparing an enzyme-labeled ligand for use in specific binding assays and the labeled conjugate produced thereby
US4258001A (en) 1978-12-27 1981-03-24 Eastman Kodak Company Element, structure and method for the analysis or transport of liquids
JPS55164356A (en) 1979-06-08 1980-12-22 Fuji Photo Film Co Ltd Multi-layer analysis sheet for liquid sample analysis
JPS6021370B2 (en) 1979-11-05 1985-05-27 富士写真フイルム株式会社 photographic material
JPS5767860A (en) 1980-10-15 1982-04-24 Fuji Photo Film Co Ltd Material for multilayer analysis
JPS57101761A (en) 1980-12-17 1982-06-24 Konishiroku Photo Ind Co Ltd Analyzing element
US4517288A (en) 1981-01-23 1985-05-14 American Hospital Supply Corp. Solid phase system for ligand assay
JPS57200862A (en) 1981-06-05 1982-12-09 Fuji Photo Film Co Ltd Mutilayer analysis element utilizing unique binding reaction
JPS5845557A (en) 1981-09-14 1983-03-16 Yatoron:Kk Reagent for quantitative determination of hydrogen peroxide
DE3225331A1 (en) 1982-07-07 1984-01-12 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR DETERMINING DIRECT AND TOTAL BILIRUBIN, AND REAGENT SUITABLE FOR THIS
JPS5925699A (en) 1982-08-03 1984-02-09 Torii Yakuhin Kk Determination of enzymatic activity
DE3463395D1 (en) 1983-02-11 1987-06-04 Nat Res Dev Enhanced luminescent or luminometric assay
JPS59193352A (en) 1983-04-18 1984-11-01 Fuji Photo Film Co Ltd Reagent for analysis, method of analysis and element of multiple chemical analysis
US4670385A (en) 1984-05-21 1987-06-02 Eastman Kodak Company Compositions and elements containing triarylmethane leuco dyes and methods using same
GB8420053D0 (en) 1984-08-07 1984-09-12 Secr Social Service Brit Enhanced luminescent/luminometric assay
GB8514288D0 (en) * 1985-06-06 1985-07-10 Amersham Int Plc Enzyme assay of body fluids
US4670381A (en) 1985-07-19 1987-06-02 Eastman Kodak Company Heterogeneous immunoassay utilizing horizontal separation in an analytical element
US4828983A (en) 1986-07-10 1989-05-09 Eastman Kodak Company Use of phenols and anilines to increase the rate of peroxidase catalyzed oxidation of leuco dyes
US4997772A (en) 1987-09-18 1991-03-05 Eastman Kodak Company Water-insoluble particle and immunoreactive reagent, analytical elements and methods of use
US4828978A (en) 1987-09-18 1989-05-09 Eastman Kodak Company Agglutination reagent and method of preparing same
US5177023A (en) 1987-08-03 1993-01-05 Eastman Kodak Company Water-insoluble reagent elements containing same and methods of use
US4892817A (en) 1987-09-21 1990-01-09 Biogenex Laboratories Stable phosphatase substrate composition
DE69026851T2 (en) 1989-02-14 1996-10-31 Wako Pure Chem Ind Ltd Process for increasing chemiluminescence
US5147777A (en) 1990-06-18 1992-09-15 Eastman Kodak Company Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use
US5162219A (en) 1990-06-18 1992-11-10 Eastman Kodak Company Preparation of amine-enriched proteins having an increased isoelectric point
CA2062176A1 (en) 1991-06-07 1992-12-08 Glen M. Dappen Use of the gravure process for coating thin film layers
ATE204385T1 (en) 1991-06-07 2001-09-15 Johnson & Johnson Clin Diag IMMUNOASSAYS USING LABELED HAPTEN ANALOGUES
US5958339A (en) * 1992-08-31 1999-09-28 Clinical Diagnostic Systems, Inc. Format for immunoassay in thin film
US5372932A (en) 1992-12-22 1994-12-13 Eastman Kodak Company Analytical element and method for the determination of a specific binding ligand using a 4-hydroxy or 4-alkoxyarylacetamide as stabilizer

Also Published As

Publication number Publication date
EP0693552B1 (en) 2003-04-02
ATE236246T1 (en) 2003-04-15
CA2154013C (en) 2006-12-19
AU2492695A (en) 1996-02-01
EP0693552A3 (en) 1996-04-03
AU697785B2 (en) 1998-10-15
US5776714A (en) 1998-07-07
JP3715348B2 (en) 2005-11-09
DE69530148T2 (en) 2004-01-08
DE69530148D1 (en) 2003-05-08
JPH08170961A (en) 1996-07-02
EP0693552A2 (en) 1996-01-24

Similar Documents

Publication Publication Date Title
CA2262920A1 (en) Method for identifying compounds affecting rna/rna binding protein interactions
EP0281251A3 (en) A method for preparing a fecal sample composition for immunoassay testing
CA2154013A1 (en) Analytical element, composition and method using modified apo-horseradish peroxidase
EP0747703A3 (en) Method for analyzing biological active substances
Kosakowski et al. The Subunit Structure of Phenylalanyl‐tRNA Synthetase of Escherichia coli
IE822628L (en) Fenclofenac as tbp blocking agent in iodothyroneine¹immunoassays
AU586666B2 (en) Proteins immobilised on polyamides or cellulose hydrate and the use thereof for the preparation of biocatalysts, test strips or chromatography materials
CA2328805A1 (en) Enzyme-protein complex
ES2148144T3 (en) PROCEDURE FOR THE STABILIZATION OF ENZYME CONJUGATES.
CA2112776A1 (en) Process for inhibiting activity of endotoxin
ATE143370T1 (en) ADHESIVE FORMULATION FOR BINDING PROTEINS
Kitahara et al. Immunological relationships among subunits of glutathione S-transferases A, AA, B and ligandin and hybrid formation between AA and ligandin by guanidine hydrochloride
WO2002048403A3 (en) Multiplexed protein expression and activity assay
CA2175061A1 (en) Muteins of Beta-Galactosidase Fragments Having Increased Activity
EP1591790A3 (en) Method and reagents for determining protein S.
CA2019872A1 (en) Method and kit for determining the functional activity of protein s in human plasma
Vilanova et al. Urea‐gradient gel electrophoresis studies on the association of procarboxypeptidases A and B, proproteinase E, and their tryptic activation products
DE3851304D1 (en) Method and test composition for the determination of enzyme activity.
Labbe et al. Proximity of alkali light chains to 27K domain of the heavy chain in myosin subfragment 1
Kasper Limited trypsin-catalyzed hydrolysis of crystalline human ceruloplasmin. Partial characterization of the digest
CA2309838A1 (en) Haptoglobin assay
Mar et al. A study of the high molecular weight hemolysin from the skin secretion of the ambhibian Bombina variegata
WO1999006446A3 (en) Method for transglutaminase-catalyzed coupling of protein or peptide to a support
Horinishi et al. A sensitive and reproducible procedure for the assay of arginyl-tRNA: protein arginyl transferase
DE3484912D1 (en) PEPTIDE DERIVATIVES AND THE USE THEREOF AS SUBSTRATES FOR THE QUANTITATIVE DETERMINATION OF ENZYMES.

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20150717