CA2169645A1 - Non-nucleotide containing enzymatic nucleic acid - Google Patents
Non-nucleotide containing enzymatic nucleic acidInfo
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- CA2169645A1 CA2169645A1 CA002169645A CA2169645A CA2169645A1 CA 2169645 A1 CA2169645 A1 CA 2169645A1 CA 002169645 A CA002169645 A CA 002169645A CA 2169645 A CA2169645 A CA 2169645A CA 2169645 A1 CA2169645 A1 CA 2169645A1
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- ribozyme
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- nucleic acid
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- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
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- C07H19/06—Pyrimidine radicals
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- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24017—Stromelysin 1 (3.4.24.17)
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Abstract
Enzymatic nucleic acid molecule containing one or more non-nucleotide mimetics, and having activity to cleave an RNA or DNA
molecule.
molecule.
Description
4 ~
~W095/0673~ PCT~S94109342 DESCRIPTION
NON-NUCLEOTIDE CONTAINING ENZYMATIC NUCLEIC ACID
Backqround of the Invention This application is a continuation-in-part of Usman et al., U.S. Serial No. 08/lS2,481, filed November 12, 1993 which is a continuation-in-part of Usman, U.S.
Serial No. 08/116,177, filed September 2, 1993, both entitled "Non-Nucleotide Containing Enzymatic Nucleic Acid" both hereby incorporated by reference herein (including drawings).
This invention relates to chemically synthesized non-nucleotide-containing enzymatic nucleic acid.
The following is a brief history of the discovery and activity of enzymatic RNA molecules or ribozymes. This history is not meant to be complete but is provided only for understanding of the invention that follows. This summary is not an admission that all of the work described below is prior art to the claimed inventlon .
Prior to the 1970s it was thought that all genes were direct linear representations of the proteins that they encoded. This simplistic view implied that all genes were like ticker tape messages, with each triplet of DNA
"letters" representing one protein "word" in the translation. Protein synthesis occurred by first transcribing a gene from DNA into RNA (letter for letter) and then translating the RNA into protein (three letters at a time). In the mid 1970s it was discovered that some genes were not exact, linear representations of the proteins that they encode. These genes were found to contain interruptions in the coding sequence which were removed from, or "spliced out" of, the RNA before it became translated into protein. These interruptions in WO95/06731 PCT~S94/09342 ~ 6~45 the coding sequence were given the name of intervening sequences (or introns) and the process of removing them from the RNA was termed splicing. At least three different mechanisms have been discovered for removing introns from RNA. Two of these splicing mechanisms involve the binding of multiple protein factors which then act to correctly cut and join the RNA. A third mechanism involves cutting and joining of the RNA by the intron itself, in what was the first discovery of catalytic RNA
molecules.
Cech and colleagues were trying to understand how RNA splicing was accomplished in a single-celled pond organism called Tetrahymena thermophila. Cech proved that the intervening sequence RNA was acting as its own splicing factor to snip itself out of the surrounding RNA.
Continuing studies in the early 1980's served to elucidate the complicated structure of the Tetrahymena intron and to decipher the mechanism by which self-splicing occurs.
Many research groups helped to demonstrate that the specific folding of the Tetrahymena intron is critical for bringing together the parts of the RNA that will be cut and spliced. Even after splicing is complete, the released intron maintains its catalytic structure. As a consequence, the released intron is capable of carrying out additional cleavage and splicing reactions on itself (to form intron circles). By 1986, Cech was able to show that a shortened form of the Tetrahymena intron could carry out a variety of cutting and joining reactions on other pieces of RNA. The demonstration proved that the Tetrahymena intron can act as a true enzyme: (i) each intron molecule was able to cut many substrate molecules while the intron molecule remained unchanged, and (ii) reactions were specific for RNA molecules that contained a unique sequence (CUCU) which allowed the intron to recognize and bind the RNA. Zaug and Cech coined the term "ribozyme" to describe any ribonucleic acid molecule that has enzyme-like properties.
095/06731 ~ 4 5 PCT~S94/09342 Also in 1986, Cech showed that the RNA substrate sequence recognized by the Tetrahymena ribozyme could be changed by altering a sequence within the ribozyme itself.
This property has led to the development of a number of site-specific ribozymes that have been individually designed to cleave at other RNA sequences.
The Tetrahymena intron is the most well-studied of what is now recognized as a large class of introns, Group I introns. The overall folded structure, including several sequence elements, is conserved among the Group I
introns, as is the general mechanism of splicing. Like the Tetrahymena intron, some members of this class are catalytic, i.e., the intron itself is capable of the self-splicing reaction. Other Group I introns require additional (protein) factors, presumably to help the intron fold into and/or maintain its active structure.
Ribonuclease P (RNaseP) is an enzyme comprised of both RNA and protein components which are responsible for converting precursor tRNA molecules into their final form by trimming extra RNA off one of their ends. RNaseP
activity has been found in all organisms tested. Sidney Altman and his colleagues showed that the RNA component of RNaseP is essential for its processing activity; however, they also showed that the protein component also was required for processing under their experimental conditions. After Cech's discovery of self-splicing by the Tetrahymena intron, the requirement for both protein and RNA components in RNaseP was reexamined. In 1983, Altman and Pace showed that the RNA was the enzymatic component of the RNaseP complex. This demonstrated that an RNA molecule was capable of acting as a true enzyme, processing numerous tRNA molecules without itself undergoing any change.
The folded structure of RNaseP RNA has been determined, and while the sequence is not strictly conserved between RNAs from different organisms, this higher order structure is. It is thought that the protein WO95/06731 PCT~S94/09342 2 1 ~
component of the RNaseP complex may serve to stabilize the folded RNA in vivo.
Symons and colleagues identified two examples of a self-cleaving RNA that differed from other forms of catalytic RNA already reported. Symons was studying the propagation of the avocado sunblotch viroid (ASV), an RNA
virus that infects avocado plants. Symons demonstrated that as little as 55 nucleotides of the ASV RNA was capable of folding in such a way as to cut itself into two pieces. It is thought that in vivo self-cleavage of these RNAs is responsible for cutting the RNA into single genome-length pieces during viral propagation. Symons discovered that variations on the minimal catalytic sequence from ASV could be found in a number of other plant pathogenic RNAs as well. Comparison of these sequences revealed a common structural design consisting of three stems and loops connected by a central loop containing many conserved (invariant from one RNA to the next) nucleotides. The predicted secondary structure for this catalytic RNA reminded the researchers of the head of a hammer; thus it was named as such.
Uhlenbeck was successful in separating the catalytic region of the ribozyme from that of the substrate. Thus, it became possible to assemble a hammerhead ribozyme from 2 (or 3) small synthetic RNAs.
A 19-nucleotide catalytic region and a 24-nucleotide substrate were sufficient to support specific cleavage.
The catalytic domain of numerous hammerhead ribozymes have now been studied by both the Uhlenbeck's and Symons' groups with regard to defining the nucleotides required for specific assembly and catalytic activity, and determining the rates of cleavage under various conditions.
Haseloff and Gerlach showed it was possible to divide the domains of the hammerhead ribozyme in a different manner. By doing so, they placed most of the required sequences in the strand that did not get cut (the ~ 095/06731 2 1 6 ~ ~ 4 5 PCT~S94/09342 ribozyme) and only a required UH where H = C, A, or U in the strand that did get cut (the substrate). This resulted in a catalytic ribozyme that could be designed to cleave any UH RNA sequence embedded within a longer "substrate recognition" sequence. The specific cleavage of a long mRNA, in a predictable manner using several such hammerhead ribozymes, was reported in 1988.
One plant pathogen RNA (from the negative strand of the tobacco ringspot virus) undergoes self-cleavage but cannot be folded into the consensus hammerhead structure described above. Bruening and colleagues have independently identified a 50-nucleotide catalytic domain for this RNA. In 1990, Hampel and Tritz succeeded in dividing the catalytic domain into two parts that could act as substrate and ribozyme in a multiple-turnover, cutting reaction. As with the hammerhead ribozyme, the catalytic portion contains most of the sequences required for catalytic activity, while only a short sequence (GUC
in this case) is required in the target. Hampel and Tritz described the folded structure of this RNA as consisting of a single hairpin and coined the term "hairpin" ribozyme (Bruening and colleagues use the term "paperclip" for this ribozyme motif). Continuing experiments suggest an increasing number of similarities between the hairpin and hammerhead ribozymes in respect to both binding of target RNA and mechanism of cleavage.
Hepatitis Delta Virus (HDV) is a virus whose genome consists of single-stranded RNA. A small region (about 80 nucleotides) in both the genomic RNA, and in the complementary anti-genomic RNA, is sufficient to support self-cleavage. In 1991, Been and Perrotta proposed a secondary structure for the HDV RNAs that is conserved between the genomic and anti-genomic RNAs and is necessary for catalytic activity. Separation of the HDV RNA into "ribozyme" and "substrate" portions has recently been achieved by Been. Been has also succeeded in reducing the size of the HDV ribozyme to about 60 nucleotides.
WO95/06731 PCT~S94/09342 ~16~
The table below lists some of the characteristics of the ribozymes discussed above:
Characteristics of Ribozymes Group I Introns Size: ~300 to >lO00 nucleotides.
Re~uires a U in the target sequence immediately 5' of the cleavage site.
Binds 4-6 nucleotides at 5' side of the cleavage site.
Over lO0 known members of this class. Found in Tetrahymena thermophila rRNA, fungal mitochondria, chloroplasts, phage T4, blue-green algae, and others.
RNaseP RN~ ~Ml RN~) Size: -290 to 400 nucleotides.
RNA portion of a ribonucleoprotein enzyme. Cleaves tRNA precursors to form mature tRNA.
Roughly lO known members of this group all are bacterial in origin.
Hammerhead Ribozyme Size: ~13 to 40 nucleotides.
Requires the target sequence UH immediately 5' of the cleavage site.
Binds a variable number nucleotides on both sides of the cleavage site.
14 known members of this class. Found in a number of plant pathogens (virusoids) that use RNA as the infectious agent. (Figure 1) Hairpin Ribozyme Size: -50 nucleotides.
Requires the target sequence GUC immediately 3' of the cleavage site.
Binds 4 nucleotides at 5' side of the cleavage site and a variable number to the 3' side of the cleavage site.
Only 1 known member of this class. Found in one plant pathogen (satellite RNA of the tobacco ringspot WO95/06731 2 ~ PCT~S94/09342 virus) which uses RNA as the infectious agent.
(Figure 2) Hepatitis Delta Virus (HDV) Ribozyme Size: ~60 nucleotides (at present).
Cleavage of target RNAs recently demonstrated.
Sequence requirements not fully determined.
Binding sites and structural requirements not fully determined, although no sequences 5' of cleavage site are required.
Only 1 known member of this class. Found in human HDV. (Figure 3) Eckstein et al., International Publication No.
WO 92/07065; Perrault et al., Nature 1990, 344:565; Pieken et al., Science 1991, 253:314; Usman and Cedergren, Trends in Biochem. sci. 1992, 17:334; Usman et al ., International Publication No. Wo 93/15187; and Rossi et al., International Publication No. WO 91/03162, describe various chemical modifications that can be made to the sugar moieties of enzymatic nucleic acid molecules.
Summarv of the Invention This invention concerns the use of non-nucleotide molecules as spacer elements at the base of double-stranded nucleic acid (e . g., RNA or DNA) stems (duplex stems) or in the single-stranded regions, catalytic core, loops, or recognition arms of enzymatic nucleic acids. Duplex stems are ubiquitous structural elements in enzymatic RNA molecules. To facilitate the synthesis of such stems, which are usually connected via single-stranded nucleotide chains, a base or base-pair mimetic may be used to reduce the nucleotide requirement in the synthesis of such molecules, and to confer nuclease resistance (since they are non-nucleic acid components).
This also applies to both the catalytic core and recognition arms of a ribozyme.
Examples of such non-nucleotide mimetics are shown in Figure 4 and their incorporation into hammerhead ~:L69~
WO95/06731 PCT~S94/09342 ribozymes is shown in Figure 5. These non-nucleotide linkers may be either polyether, polyamine, polyamide, or polyhydrocarbon compounds. Specific examples include those described by Seela and Kaiser, Nucleic Acids Res.
1990, 18:6353 and Nucleic Acids Res. 1987, 15:3113; Cload and Schepartz, J. Am. Chem. Soc. 1991, 113:6324;
Richardson and Schepartz, J. Am. Chem. Soc. 1991, 113 : 5109 ; Ma et al., Nucleic Acids Res. 1993, 21 : 2585 and Biochemistry 1993, 32: 1751; Durand et al., Nucleic Acids Res. l99O,18: 6353; McCurdy et al., Nucleosides &
Nucleotides 1991, lo : Z87 ; Jaschke et al., Tetrahedron Lett. 1993, 34:301; Ono et al., Biochemistry 1991, 30 : 9914 ; Arnold et al., International Publication No.
Wo 89/02439 entitled "Non-nucleotide Linking Reagents for 15 Nucleotide Probes"; and Ferentz and Verdine, ~ . Chem.
Soc. 1991, 113: 4000, all hereby incorporated by reference herein.
Thus, in a first aspect, the invention features an enzymatic nucleic acid molecule having one or more 20 non-nucleotide moieties, and having enzymatic activity to cleave an RNA or DNA molecule.
Examples of such non-nucleotide mimetics are shown in Figure 4 and their incorporation into hammerhead ribozymes is shown in Figure 5. These non-nucleotide linkers may be either polyether, polyamine, polyamide, or polyhydrocarbon compounds.
In preferred embodiments, the enzymatic nucleic acid includes one or more stretches of RNA, which provide the enzymatic activity of the molecule, linked to the non-nucleotide moiety.
By the term "non-nucleotide" is meant any group or compound which can be incorporated into a nucleic acid chain in the place of one or more nucleotide units, including either sugar and/or phosphate substitutions, and allows the remaining bases to exhibit their enzymatic activity. The group or compound is abasic in that it does not contain a commonly recognized nucleotide base, such as ~WO95/06731 2 ~ 6 ~ ~ ~ S PCT~S94/09342 adenosine, guanine, cytosine, uracil or thymine. It may have substitutions for a 2' or 3' H or OH as described in the art. See Eckstein et al. and Usman et al., supra.
In preferred embodiments, the enzymatic nucleic acid includes one or more stretches of RNA, which provide the enzymatic activity of the molecule, linked to the non-nucleotide moiety. The necessary RNA components are known in the art, see, e.g., Usman, sup~a.
As the term is used in this application, non-nucleotide-containing enzymatic nucleic acid means a nucleic acid molecule that contains at least one non-nucleotide component which replaces a portion of a ribozyme, e.g., but not limited to, a double-stranded stem, a single-stranded "catalytic core" sequence, a single-stranded loop or a single-stranded recognition sequence. These molecules are able to cleave (preferably, repeatedly cleave) separate RNA or DNA molecules in a nucleotide base sequence specific manner. Such molecules can also act to cleave intramolecularly if that is desired. Such enzymatic molecules can be targeted to virtually any RNA transcript. Such molecules also include nucleic acid molecules having a 3' or 5' non-nucleotide, useful as a capping group to prevent exonuclease digestion.
Enzymatic molecules of this invention act by first binding to a target RNA or DNA. Such binding occurs through the target binding portion of the enzyme which is held in close proximity to an enzymatic portion of molecule that acts to cleave the target RNA or DNA. Thus, the molecule first recognizes and then binds a target nucleic acid through complementary base-pairing, and once bound to the correct site, acts enzymatically to cut the target. Strategic cleavage of such a target will destroy its ability to direct synthesis of an encoded protein.
After an enzyme of this invention has bound and cleaved its target it is released from that target to search for W O 95/06731 . PCTrUS94/09342 9 ~
another target, and can repeatedly bind and cleave new targets.
The enzymatic nature of an enzyme of this invention is advantageous over other technologies, such as antisense technology (where a nucleic acid molecule simply binds to a nucleic acid target to block its translation) since the effective concentration of the enzyme necessary to effect a therapeutic treatment is lower than that of an antisense oligonucleotide. This advantage reflects the ability of the enzyme to act enzymatically. Thus, a single enzyme molecule is able to cleave many molecules of target RNA. In addition, the enzyme is a highly specific inhibitor, with the specificity of inhibition depending not only on the base pairing mechanism of binding, but also on the mechanism by which the molecule inhibits the expression of the RNA to which it binds. That is, the inhibition is caused by cleavage of the target and so specificity is defined as the ratio of the rate of cleavage of the targeted nucleic acid over the rate of cleavage of non-targeted nucleic acid. This cleavage mechanism is dependent upon factors additional to those involved in base pairing. Thus, it is thought that the specificity of action of an enzyme of this invention is greater than that of antisense oligonucleotide binding the same target site.
By the phrase enzyme is meant a catalytic non-nucleotide-containing nucleic acid molecule that has complementarity in a substrate-binding region to a specified nucleic acid target, and also has an enzymatic activity that specifically cleaves RNA or DNA in that target. That is, the enzyme is able to intramolecularly or intermolecularly cleave RNA or DNA and thereby inactivate a target RNA or DNA molecule. This complementarity functions to allow sufficient hybridization of the enzymatic molecule to the target RNA
or DNA to allow the cleavage to occur. One hundred 095/06731 ~ 6 4 5 PCT~S94/09342 percent complementarity is preferred, but complementarity as low as 50-75~ may also be useful in this invention.
In preferred embodiments of this invention, the enzyme molecule is formed generally in a hammerhead motif, but may also be formed in the motif of a hairpin, hepatitis delta virus, group I intron or RNaseP RNA (in association with an RNA guide sequence). Examples of such hammerhead motifs are described by Rossi et al., Aids Research and Human Retroviruses 1992, 8:183; of hairpin motifs by Hampel et al., "RNA Catalyst for Cleaving Specific RNA Sequences," filed September 20, 1989, which is a continuation-in-part of U.S. Serial No. 07/247,100 filed September 20, 1988, Hampel and Tritz, Biochemistry 1989, 28:4929, and Hampel et al ., Nucleic Acids Research l99o, 18:299; and an example of the hepatitis delta virus motif is described by Perrotta and Been, Biochemistry 1992, 31:16; of the RNaseP motif by Guerrier-Takada et al ., Cell 1983, 35:849; and of the Group I intron by Cech et al , U. S. Patent 4,987,071. These specific motifs are not limiting in the invention and those skilled in the art will recognize that all that is important in an enzyme molecule of this invention is that it have at least one non-nucleotide portion, and a specific substrate-binding site which is complementary to one or more of the target gene RNA regions, and that it have nucleotide sequences within or surrounding that substrate-binding site which impart a nucleic acid cleaving activity to the molecule.
The invention provides a method for producing a class of enzymatic cleaving agents which exhibit a high degree of specificity for the nucleic acid of a desired target. The enzyme molecule is preferably targeted to a highly conserved sequence region of a target such that specific treatment of a disease or condition can be provided with a single enzyme. Such enzyme molecules can be delivered exogenously to specific cells as required.
In the preferred hammerhead motif the small size (less than 60 nucleotides, preferably between 30-40 nucleotides WO95/06731 ~ PCT~S94/09342 in length) of the molecule allows the cost of treatment to be reduced compared to other ribozyme motifs.
Synthesis of nucleic acids greater than lOO
nucleotides in length is difficult using automated methods, and the therapeutic cost of such molecules is prohibitive. In this invention, small enzyme motifs (e.g., of the hammerhead structure) are used for exogenous delivery. The simple structure of these molecules increases the ability of the enzyme to invade targeted regions of mRNA structure. Unlike the situation when the hammerhead structure is included within longer transcripts, there are no non-enzyme flanking sequences to interfere with correct folding of the enzyme structure or with complementary regions.
Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims.
Descri~tion of the Preferred Embodiments The drawings will first briefly be described.
ZO Drawinqs:
Figure l is a diagrammatic representation of the hammerhead ribozyme domain known in the art.
Figure 2 is a diagrammatic representation of the general structure of the hairpin ribozyme domain known in the art.
Figure 3 is a diagrammatic representation of the general structure of the hepatitis delta virus ribozyme domain known in the art.
Figure 4 is a diagrammatic representation of various non-nucleotide mimetics that may be incorporated into nucleic acid enzymes. Standard abbreviations are used in the Figure. In compound l each X may independently be oxygen, nitrogen, sulfur or substituted carbons containing alkyl, alkene or equivalent chains of length l-lO carbon atoms. In compounds 6, 6a, 7, 8, 9 and lO each Y may independently be a phosphodiester, ether or amide linkage to the rest of the nucleic acid enzyme. In 095/06731 2 1 6 ~ PCT~S94/09342 compounds 4 and 5 each R may independently be H, OH, protected OH, O-alkyl, alkenyl or alkynyl or alkyl, alkenyl or alkynyl of l-lO carbon atoms.
Figure 5 is a diagrammatic representation of the preferred location for incorporation of various non-nucleotide mimetics into nucleic acid enzymes.
Specifically, mimetics, l-lO, may replace the loop (denoted as / / in Figure 5) that connects the two strands of Stem II. Stem II itself may be from l to lO
base pairs. In examples l ~ 2 below compounds l and 2 were incorporated into molecules having a stem II of l to 5 basepairs in length. Compounds l, 4 and 5 may also replace nucleotides in the recognition arms of stems I and III or in stem II itself.
Figure 6 is a diagrammatic representation of the synthesis of a perylene based non-nucleotide mimetic phosphoramidite 3.
Figure 7 is a diagrammatic representation of the synthesis of an abasic deoxyribose or ribose non-nucleotide mimetic phosphoramidite.
Figures 8a and 8b are graphical representations of cleavage of substrate by various ribozymes at 8nM, or 40 nM, respectively.
Non-nucleotide Mimetics Non-nucleotide mimetics useful in this invention are generally described above. Those in the art will recognize that these mimetics can be incorporated into an enzymatic molecule by standard techniques at any desired location. Suitable choices can be made by standard experiments to determine the best location, e.g., by synthesis of the molecule and testing of its enzymatic activity. The optimum molecule will contain the known ribonucleotides needed for enzymatic activity, and will have non-nucleotides which change the structure of the molecule in the least way possible. What is desired is that several nucleotides can be substituted by one non-nucleotide to save synthetic steps in enzymatic molecule ~169~5 . `
WO95/06731 PCT~S94/09342 synthesis and to provide enhanced stability of the molecule compared to RNA or even DNA.
Examples The following are non-limiting examples showing the synthesis of non-nucleotide mimetic-containing catalytic nucleic acids using non-nucleotide phosphoramidites.
Example 1: SYnthesis of Hammerhead Ribozymes Containinq Non-nucleotide Mimetics: PolYether SPaCers Polyether spacers, compound 1 (Figure 4; X-O, n=2 or 4), have been incorporated both singly, n=2 or 4, or doubly, n=2, at the base of stem II of a hammerhead ribozyme, replacing loop 2, and shown to produce a ribozyme which has lower catalytic efficiency. The method of synthesis used followed the procedure for normal RNA
synthesis as described in Usman et al., J. Am. Chem. Soc.
1987, ld9:7845 and in Scaringe et al., Nucleic Acids ~es .
1990, 18:5433, and makes use of common nucleic acid protecting and coupling groups, such as dimethoxytrityl at the 5'-end, and phosphoramidites at the 3'-end. The average stepwise coupling yields were >98%. The design of these types of mimetics has not been optimized to date, but, as discussed above, this can be readily achieved using standard experimental techniques. These experiments indicate the potential of such mimetics to replace the loops and portions of stems in ribozymes while maintaining catalytic activity. These mimetics may be incorporated not only into hammerhead ribozymes, but also into hairpin, hepatitis delta virus, or Group 1 or Group 2 introns.
They are, therefore, of general use as replacement motifs in any nucleic acid structure. Use of such mimetics allows about 2-10 nucleotides to be omitted from the final nucleic acid molecule compared to the use of an oligonucleotide without a non-nucleotide mimetic.
ExamPle 2: SYnthesis of Hammerhead RibozYmes Containinq Non-nucleotide Mimetics: Aromatic SPacers ~WO95/06731 2 ~ 3 PCT~S94/09342 -In another example, a specific linker for the base of the stem II C-G of a hammerhead ribozyme was designed. Applicant believes that the distance between the C1' carbons of the C-G base pair is about 16 Angstroms. To join these two pieces of RNA by a covalent analog of the C-G base pair a new type of dimer phosphoramidite containing a linker between the 3'-OH and the 5'-OH of the G and C residues respectively can be constructed. Two types of base-pair mimetic are the rigid aromatic spacers, 2 or 3, shown in Figure 4. These have been incorporated at the base of stem II of a hammerhead ribozyme as described in Example 1, replacing loop 2, and shown to produce a ribozyme which has lower catalytic efficiency. Another mimetic is a flexible alkyl spacer similar to the polyamide backbone described by Nielsen et al., Science 1991, 254:1497 (see, Figure 4; 6 or a derivative thereof 6a; Zuckerman et al., J. Am. Chem. Soc.
1992, 114:10464). Use of such mimetics allows about 2-10 nucleotides to be omitted from the final nucleic acid molecule compared to the use of an oligonucleotide without a non-nucleotide mimetic.
ExamPle 3: SYnthesis of Non-nucleotide Mimetics Aromatic Spacer Phosphoramidite 2 This compound was originally described by Salunkhe et al., J. Am. Chem. Soc. 1992, 114:6324. The synthesis was modified as follows: To terphthalic acid (1.0 g, 6.0 mmol) in DMF (12 mL) was added EDC (2.54 g, 13.2 mmol), aminohexanol (1.55 g, 13.2 mmol) and N-methylmorpholine (1.45 mL, 13.2 mmol). The reaction mixture was stirred overnight at which time the solution was cloudy. Water was added to the reaction mixture to precipitate out the product. The solid was filtered and washed with water and dried to provide 562 mg (25.7~) of the diol.
To the diol (250 mg, 0.687 mmol) in DMSO (40 mL) was added triethylamine (287 ~L, 2.06 mmol), dimethoxytrityl chloride (220 mg, 0.653 mmol) and WO95/06731 PCT~S94/09342 -catalytic DMAP. The reaction mixture was heated to 40C
and stirred overnight. The mixture was then cooled to room temperature (about 20-25C), quenched with water and extracted three times with EtOAc. A solid precipitate remained in the organic layer that was isolated and found to be starting diol (50 mg, 20~). The organic layer was dried over Na2SO4 and evaporated. The resulting oil was purified with flash chromatography (10% EtOAc in hexanes to 100% EtOAc) to yield 250 mg (55%) of the monotritylated compound.
To the alcohol (193 mg, 0.29 mmol) in THF (1 mL) at 0C was added diisopropylethylamine (101 ~L, 0.58 mmol)~
and then 2-cyanoethyl N,N-diisopropylamino chlorophosphoramidite (78 ~L, 0.35 mmol) dropwise. The resulting mixture was stirred for 5 minutes and then warmed to room temperature. After 1 hour the reaction mixture was quenched with methanol and evaporated. The resulting oil was purified by flash chromatography (1:1 hexanes:EtOAc) to yield 158 mg (63%) of the phosphoramidite.
ExamPle 4: SYnthesis of Non-nucleotide Mimetics Aromatic Spacer Phosphoramidite 3 Referring to Figure 6, to 3, 4, 9, 10-perylenetetracarboxylic dianhydride 11 (1.0 g, 2.55 mmol) in quinoline (10 mL) was added ethanolamine (919 ~L, 15.3 mmol) and ZnOAc-2.5 H2O (140 mg, 0.638 mmol). The reaction mixture was heated to 190C for 8 hours. The solution was then cooled, lN HCl added to precipitate the product and the mixture was filtered. The solid was washed with hot 10% CaCO3 until the filtrate was no longer pale green. The remaining bright red precipitate 12 was then dried.
The resulting diol 12 was then treated as outlined above for 2 to provide the phosphoramidite 3.
ExamPle 5: SYnthesis of Hammerhead Ribozymes Containinq Non-nucleotide Mimetics: Abasic Nucleotides 4 and s ~ 095/06731 2 1 6 9 6 4 5 PCT~S94/09342 Compound 4, R=H, was prepared according to Iyer et alO ~ Nucleic Acids Res. 1990, 18:2855. Referring to Figure 7, compounds 4 and 5 (R=O-t-butyldimethylsilyl) phosphoramidites were prepared as follows:
To a solution of D-ribose (20.0 g, 0.105 mol) in N,N-dimethylformamide (250 mL) was added 2,2-dimethoxypropane (50 mL) and p-toluenesulfonic acid monohydrate (300 mg). The reaction mixture was stirred for 16 hours at room temperature and then evaporated to dryness. The crude product was coevaporated with pyridine (2 x 150 mL), dissolved in dry pyridine (300 mL) and 4,4'-dimethoxytrityl chloride (37.2 g, 0.110 mol) was added and stirred for 24 hours at room temperature. The reaction mixture was diluted with methanol (50 mL) and evaporated to dryness. The residue was dissolved in chloroform (800 mL) and washed with 5% NaHC03 (2 x 200 mL), brine (300 mL), dried, evaporated, coevaporated with toluene (2 x 100 mL) and purified by flash chromatography in CHCI3to yield 40.7 g (78.1%) of compound a.
To a solution of dimethoxytrityl derivative a (9.o g, 18.3 mmol) and DMAP (4.34 g, 36 mmol) in dry CH3CN, phenoxythiocarbonyl chloride (3.47 g, 20.1 mmol) was added dropwise under argon. The reaction mixture was left for 16 hours at room temperature, then evaporated to dryness.
The resulting residue was dissolved in chloroform (200 mL), washed with 5% NaHCO3, brine, dried, evaporated and purified by flash chromatography in CHCI3, to yield 8.0 g (69.5%) of compound b as the ~-anomer.
To a solution of intermediate b (3.0 g, 4.77 mmol) in toluene (50 mL) was added AIBN (0.82 g, 5.0 mmol) and Bu3SnH (1.74 g, 6.0 mmol) under argon and the reaction - mixture was kept at 80C for 7 hours. The solution was evaporated and the resulting residue purified by flash - chromatography in CHCl3 to yield 1.5 g (66%) of protected ribitol c.
Subsequent removal of all protecting groups by acid treatment and tritylation provided the protected WO95/06731 ~ PCT~S94/09342 ribitol d which was then converted to target phosphoramidites 4 and 5 by the general method described in Scaringe et al., Nucleic Acids Res . 1990, 18: 5433 .
ExamPle 6 Referring to Figures 8a and 8b the cleavage of substrate is shown by various modified ribozymes compared to unmodified ribozyme at 8nM and 40nM concentrations.
Specifically, a control ribozyme of sequence ucuccA UCU
GAU GAG GCC GAA AGG CCG AAA Auc ccU (where lower case includes a 2' O-methyl group) was compared to ribozyme A
(ucu ccA UCU GAU GAG GCC SGG CCG AAA Auc ccu), B (ucu ccA
UCU GAU GAG CSG CG AAA Auc ccu), C (ucu ccA UCU GAU GAG
GCC bbb bGG CCG AAA Auc ccu), and D (ucu ccA UCU GAU GAG
Cbb bbG CGAA AAu ccc u) (where S=hexaethylene glycol linker); and b=abasic nucleotide 4). All were active in cleaving substrate.
Administration of RibozYme Selected ribozymes can be administered prophylactically, to viral infected patients or to diseased patients, e.g., by exogenous delivery of the ribozyme to a relevant tissue by means of an appropriate delivery vehicle, e.g., a liposome, a controlled release vehicle, by use of iontophoresis, electroporation or ion paired molecules, or covalently attached adducts, and other pharmacologically approved methods of delivery.
Routes of administration include intramuscular, aerosol, oral (tablet or pill form), topical, systemic, ocular, intraperitoneal and/or intrathecal.
The specific delivery route of any selected ribozyme will depend on the use of the ribozyme.
Generally, a specific delivery program for each ribozyme will focus on unmodified ribozyme uptake with regard to intracellular localization, followed by demonstration of efficacy. Alternatively, delivery to these same cells in an organ or tissue of an animal can be pursued. Uptake studies will include uptake assays to evaluate cellular ribozyme uptake, regardless of the delivery vehicle or ~ 095/06731 216 ~ ~ ~ 5 PCT~S94/09342 , strategy. Such assays will also determine the intracellular localization of the ribozyme following uptake, ultimately establishing the requirements for maintenance of steady-state concentrations within the 5 cellular compartment containing the target sequence (nucleus and/or cytoplasm). Efficacy and cytotoxicity can then be tested. Toxicity will not only include cell viability but also cell function.
Some methods of delivery that may be used 10 include:
a. encapsulation in liposomes, b. transduction by retroviral vectors, c. conjugation with cholesterol, d. localization to nuclear compartment utilizing antigen binding or nuclear targeting site found on most snRNAs or nuclear proteins, e. neutralization of charge of ribozyme by using nucleotide derivatives, and f. use of blood stem cells to distribute ribozymes throughout the body.
Delivery strategies useful in the present invention, include: ribozyme modifications, and particle carrier drug delivery vehicles. Unmodified ribozymes, like most small molecules, are taken up by cells, albeit slowly. To enhance cellular uptake, the ribozyme may be modified essentially at random, in ways which reduce its charge but maintains specific functional groups. This results in a molecule which is able to diffuse across the cell membrane, thus removing the permeability barrier.
Modification of ribozymes to reduce charge is just one approach to enhance the cellular uptake of these larger molecules. The random approach, however, is not advisable since ribozymes are structurally and functionally more complex than small drug molecules. The structural requirements necessary to maintain ribozyme catalytic activity are well understood by those in the W095/06731 ~ PCT~S94/09342 æ~9~
art. These requirements are taken into consideration when designing modifications to enhance cellular delivery. The modifications are also designed to reduce susceptibility to nuclease degradation. Both of these characteristics should greatly improve the efficacy of the ribozyme.
Cellular uptake can be increased by several orders of magnitude without having to alter the phosphodiester linkages necessary for ribozyme cleavage activity.
Chemical modifications of the phosphate backbone will reduce the negative charge allowing free diffusion across the membrane. This principle has been successfully demonstrated for antisense DNA technology. The similarities in chemical composition between DNA and RNA
make this a feasible approach. In the body, maintenance of an external concentration will be necessary to drive the diffusion of the modified ribozyme into the cells of the tissue. Administration routes which allow the diseased tissue to be exposed to a transient high concentration of the drug, which is slowly dissipated by systemic adsorption are preferred. Intravenous administration with a drug carrier designed to increase the circulation half-life of the ribozyme can be used.
The size and composition of the drug carrier restricts rapid clearance from the blood stream. The carrier, made to accumulate at the site of infection, can protect the ribozyme from degradative processes.
Drug delivery vehicles are effective for both systemic and topical administration. They can be designed to serve as a slow release reservoir, or to deliver their contents directly to the target cell. An advantage of using direct delivery drug vehicles is that multiple molecules are delivered per uptake. Such vehicles have been shown to increase the circulation half-life of drugs which would otherwise be rapidly cleared from the blood stream. Some examples of such specialized drug delivery vehicles which fall into this category are liposomes, ~WO95/06731 21~ PCT~S94109342 hydrogels, cyclodextrins, biodegradable nanocapsules, and bioadhesive microspheres.
From this category of delivery systems, liposomes are preferred. Liposomes increase intracellular stability, increase uptake efficiency and improve biological activity.
Liposomes are hollow spherical vesicles composed of lipids arranged in a similar fashion as those lipids which make up the cell membrane. They have an internal aqueous space for entrapping water soluble compounds and range in size from 0.05 to several microns in diameter.
Several studies have shown that liposomes can deliver RNA
to cells and that the RNA remains biologically active.
For example, a liposome delivery vehicle originally designed as a research tool, Lipofectin, has been shown to deliver intact mRNA molecules to cells yielding production of the corresponding protein. In another study, an antibody targeted liposome delivery system containing an RNA molecule 3,500 nucleotides in length and antisense to a structural protein of HIV, inhibited virus proliferation in a sequence specific manner. Not only did the antibody target the liposomes to the infected cells, but it also triggered the internalization of the liposomes by the infected cells.
Triggering the endocytosis is useful for viral inhibition.
Finally, liposome delivered synthetic ribozymes have been shown to concentrate in the nucleus of H9 (an example of an HIV-sensitive cell) cells and are functional as evidenced by their intracellular cleavage of the sequence.
Liposome delivery to other cell types using smaller ribozymes (less than 142 nucleotides in length) exhibit different intracellular localizations.
Liposomes offer several advantages: They are non-toxic and biodegradable in composition; they display long circulation half-lives; and recognition molecules can be readily attached to their surface for targeting to tissues. Finally, cost effective manufacture of liposome-WO95/06731 PCT~S94109342 based pharmaceuticals, either in a liquid suspension orlyophilized product, has demonstrated the viability of this technology as an acceptable drug delivery system.
Other controlled release drug delivery systems, such as nonoparticles and hydrogels may be potential delivery vehicles for a ribozyme. These carriers have been developed for chemotherapeutic agents and protein-based pharmaceuticals, and consequently, can be adapted for ribozyme delivery.
Topical administration of ribozymes is advantageous since it allows localized concentration at the site of administration with minimal systemic adsorption. This simplifies the delivery strategy of the ribozyme to the disease site and reduces the extent of toxicological characterization. Furthermore, the amount of material to be applied is far less than that required for other administration routes. Effective delivery requires the ribozyme to diffuse into the infected cells.
Chemical modification of the ribozyme to neutralize negative charge may be all that is required for penetration. However, in the event that charge neutralization is insufficient, the modified ribozyme can be co-formulated with permeability enhancers, such as Azone or oleic acid, in a liposome. The liposomes can either represent a slow release presentation vehicle in which the modified ribozyme and permeability enhancer transfer from the liposome into the infected cell, or the liposome phospholipids can participate directly with the modified ribozyme and permeability enhancer in facilitating cellular delivery. In some cases, both the ribozyme and permeability enhancer can be formulated into a suppository formulation for slow release.
Ribozymes may also be systemically administered.
Systemic absorption refers to the accumulation of drugs in the blood stream followed by distribution throughout the entire body. Administration routes which lead to systemic absorption include: intravenous, subcutaneous, ~ WO95/06731 2 1~ 9 ~ I ~ PCT~S94/09342 .
intraperitoneal, intranasal, intrathecal and ophthalmic.
Each of these administration routes expose the ribozyme to an accessible diseased tissue. Subcutaneous administration drains into a localized lymph node which proceeds through the lymphatic network into the circulation. The rate of entry into the circulation has been shown to be a function of molecular weight or size.
The use of a liposome or other drug carrier localizes the ribozyme at the lymph node. The ribozyme can be modified to diffuse into the cell, or the liposome can directly participate in the delivery of either the unmodified or modified ribozyme to the cell. This method is particularly useful for treating AIDS using anti-HIV
ribozymes.
Also preferred in AIDS therapy is the use of a liposome formulation which can deliver oligonucleotides to lymphocytes and macrophages. This oligonucleotide delivery system inhibits HIV proliferation in infected primary immune cells. Whole blood studies show that the formulation is taken up by 90~ of the lymphocytes after 8 hours at 37C. Preliminary biodistribution and pharmacokinetic studies yielded 70% of the injected dose/gm of tissue in the spLeen after one hour following intravenous administration. This formulation offers an excellent delivery vehicle for anti-AIDS ribozymes for two reasons. First, T-helper lymphocytes and macrophages are the primary cells infected by the virus, and second, a subcutaneous administration delivers the ribozymes to the resident HIV-infected lymphocytes and macrophages in the lymph node. The liposomes then exit the lymphatic system, enter the circulation, and accumulate in the spleen, where the ribozyme is delivered to the resident lymphocytes and macrophages.
Intraperitoneal administration also leads to entry into the circulation, with once again, the molecular weight or size of the ribozyme-delivery vehicle complex controlling the rate of entry.
WO95/06731 ; PCT~S94/093~2 ~
4 ~
Liposomes injected intravenously show accumulation in the liver, lung and spleen. The composition and size can be adjusted so that this accumulation represents 30% to 40% of the injected dose.
The remaining dose circulates in the blood stream for up to 24 hours.
The chosen method of delivery should result in cytoplasmic accumulation in the afflicted cells and molecules should have some nuclease-resistance for optimal dosing. Nuclear delivery may be used but is less preferable. Most preferred delivery methods include liposomes (10-400 nm), hydrogels, controlled-release polymers, microinjection or electroporation (for ex vivo treatments) and other pharmaceutically applicable vehicles. The dosage will depend upon the disease indication and the route of administration but should be between 100-200 mg/kg of body weight/day. The duration of treatment will extend through the course of the disease symptoms, usually at least 14-16 days and possibly continuously. Multiple daily doses are anticipated for topical applications, ocular applications and vaginal applications. The number of doses will depend upon disease delivery vehicle and efficacy data from clinical trials.
Establishment of therapeutic levels of ribozyme within the cell is dependent upon the rate of uptake and degradation. Decreasing the degree of degradation will prolong the intracellular half-life of the ribozyme.
Thus, chemically modified ribozymes, e.g., with modification of the phosphate backbone, or capping of the 5' and 3' ends of the ribozyme with nucleotide analogues may require different dosaging. Descriptions of useful systems are provided in the art cited above, all of which is hereby incorporated by reference herein.
For a more detailed description of ribozyme design, see, Draper, U.S. Serial No. 08/103,243 filed 095/06731 216 9 ~ 4 5 PCT~S94/09342 August 6, 1993, hereby incorporated by reference herein in its entirety.
Other embodiments are within the following claims.
~ ,i l., WO95/06731 PCT~S94/09342 ~
~69~5 "Sequence Listing"
(1) GENERAL INFORMATION:
(i) APPLICANT: Nassim Usman Francine E. Wincott Jasenka Matulic-Adamic Leonid Beigelman (ii) TITLE OF INVENTION: NON-NUCLEOTIDE CONTAINING
ENZYMATIC NUCLEIC ACID
(iii) NUMBER OF SEQUENCES: 5 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Lyon & Lyon (B) STREET: 611 West Sixth Street (C) CITY: Los Angeles (D) STATE: California (E) COUNTRY: USA
(F) ZIP: 90017 (v) CO~ .~ READABLE FORM:
(A) MEDIUM TYPE: 3.5" Diskette, 1.44 Mb storage (B) COMPUTER: IBM Compatible (C) OPERATING SYSTEM: IBM P.C. DOS (Version 5.0) (D) SOFTWARE: WordPerfect (Version 5.1) (vi) CURRENT APPLICATION DATA:
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(B) FILING DATE:
(C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
Prior applications total, including application described below: two (A) APPLICATION NUMBER: 08/152,488 (B) FILING DATE: 12 NOV 1993 (A) APPLICATION NUMBER: 08/116,177 (B) FILING DATE: 02 SEPT 1993 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: . Warburg, Richard J.
(B) REGISTRATION NUMBER: 32,327 (C) REFERENCE/DOCKET NUMBER: 206/267 ~ WO95/06731 ~ 1 ~t9'l~ 4 ~ PCT~S94/09342 , . . . .
(ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (213) 489-1600 (B) TELEFAX: (213) 955-0440 (C) TELEX: 67-3510 (2) INFORMATION FOR SEQ ID NO: 1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 11 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base. "H"
r e p r e s e n t s nucleotide C, A, or U.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 1:
(2) INFORMATION FOR SEQ ID NO: 2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 46X (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 2:
WO95106731 ;~ PCT~S94/09342 ~
~169~4~
(2) INFORMATION FOR SEQ ID NO: 3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l4 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 3:
NNNNNGUCNN NNNN l4 (2) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 50 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 4:
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~W095/0673~ PCT~S94109342 DESCRIPTION
NON-NUCLEOTIDE CONTAINING ENZYMATIC NUCLEIC ACID
Backqround of the Invention This application is a continuation-in-part of Usman et al., U.S. Serial No. 08/lS2,481, filed November 12, 1993 which is a continuation-in-part of Usman, U.S.
Serial No. 08/116,177, filed September 2, 1993, both entitled "Non-Nucleotide Containing Enzymatic Nucleic Acid" both hereby incorporated by reference herein (including drawings).
This invention relates to chemically synthesized non-nucleotide-containing enzymatic nucleic acid.
The following is a brief history of the discovery and activity of enzymatic RNA molecules or ribozymes. This history is not meant to be complete but is provided only for understanding of the invention that follows. This summary is not an admission that all of the work described below is prior art to the claimed inventlon .
Prior to the 1970s it was thought that all genes were direct linear representations of the proteins that they encoded. This simplistic view implied that all genes were like ticker tape messages, with each triplet of DNA
"letters" representing one protein "word" in the translation. Protein synthesis occurred by first transcribing a gene from DNA into RNA (letter for letter) and then translating the RNA into protein (three letters at a time). In the mid 1970s it was discovered that some genes were not exact, linear representations of the proteins that they encode. These genes were found to contain interruptions in the coding sequence which were removed from, or "spliced out" of, the RNA before it became translated into protein. These interruptions in WO95/06731 PCT~S94/09342 ~ 6~45 the coding sequence were given the name of intervening sequences (or introns) and the process of removing them from the RNA was termed splicing. At least three different mechanisms have been discovered for removing introns from RNA. Two of these splicing mechanisms involve the binding of multiple protein factors which then act to correctly cut and join the RNA. A third mechanism involves cutting and joining of the RNA by the intron itself, in what was the first discovery of catalytic RNA
molecules.
Cech and colleagues were trying to understand how RNA splicing was accomplished in a single-celled pond organism called Tetrahymena thermophila. Cech proved that the intervening sequence RNA was acting as its own splicing factor to snip itself out of the surrounding RNA.
Continuing studies in the early 1980's served to elucidate the complicated structure of the Tetrahymena intron and to decipher the mechanism by which self-splicing occurs.
Many research groups helped to demonstrate that the specific folding of the Tetrahymena intron is critical for bringing together the parts of the RNA that will be cut and spliced. Even after splicing is complete, the released intron maintains its catalytic structure. As a consequence, the released intron is capable of carrying out additional cleavage and splicing reactions on itself (to form intron circles). By 1986, Cech was able to show that a shortened form of the Tetrahymena intron could carry out a variety of cutting and joining reactions on other pieces of RNA. The demonstration proved that the Tetrahymena intron can act as a true enzyme: (i) each intron molecule was able to cut many substrate molecules while the intron molecule remained unchanged, and (ii) reactions were specific for RNA molecules that contained a unique sequence (CUCU) which allowed the intron to recognize and bind the RNA. Zaug and Cech coined the term "ribozyme" to describe any ribonucleic acid molecule that has enzyme-like properties.
095/06731 ~ 4 5 PCT~S94/09342 Also in 1986, Cech showed that the RNA substrate sequence recognized by the Tetrahymena ribozyme could be changed by altering a sequence within the ribozyme itself.
This property has led to the development of a number of site-specific ribozymes that have been individually designed to cleave at other RNA sequences.
The Tetrahymena intron is the most well-studied of what is now recognized as a large class of introns, Group I introns. The overall folded structure, including several sequence elements, is conserved among the Group I
introns, as is the general mechanism of splicing. Like the Tetrahymena intron, some members of this class are catalytic, i.e., the intron itself is capable of the self-splicing reaction. Other Group I introns require additional (protein) factors, presumably to help the intron fold into and/or maintain its active structure.
Ribonuclease P (RNaseP) is an enzyme comprised of both RNA and protein components which are responsible for converting precursor tRNA molecules into their final form by trimming extra RNA off one of their ends. RNaseP
activity has been found in all organisms tested. Sidney Altman and his colleagues showed that the RNA component of RNaseP is essential for its processing activity; however, they also showed that the protein component also was required for processing under their experimental conditions. After Cech's discovery of self-splicing by the Tetrahymena intron, the requirement for both protein and RNA components in RNaseP was reexamined. In 1983, Altman and Pace showed that the RNA was the enzymatic component of the RNaseP complex. This demonstrated that an RNA molecule was capable of acting as a true enzyme, processing numerous tRNA molecules without itself undergoing any change.
The folded structure of RNaseP RNA has been determined, and while the sequence is not strictly conserved between RNAs from different organisms, this higher order structure is. It is thought that the protein WO95/06731 PCT~S94/09342 2 1 ~
component of the RNaseP complex may serve to stabilize the folded RNA in vivo.
Symons and colleagues identified two examples of a self-cleaving RNA that differed from other forms of catalytic RNA already reported. Symons was studying the propagation of the avocado sunblotch viroid (ASV), an RNA
virus that infects avocado plants. Symons demonstrated that as little as 55 nucleotides of the ASV RNA was capable of folding in such a way as to cut itself into two pieces. It is thought that in vivo self-cleavage of these RNAs is responsible for cutting the RNA into single genome-length pieces during viral propagation. Symons discovered that variations on the minimal catalytic sequence from ASV could be found in a number of other plant pathogenic RNAs as well. Comparison of these sequences revealed a common structural design consisting of three stems and loops connected by a central loop containing many conserved (invariant from one RNA to the next) nucleotides. The predicted secondary structure for this catalytic RNA reminded the researchers of the head of a hammer; thus it was named as such.
Uhlenbeck was successful in separating the catalytic region of the ribozyme from that of the substrate. Thus, it became possible to assemble a hammerhead ribozyme from 2 (or 3) small synthetic RNAs.
A 19-nucleotide catalytic region and a 24-nucleotide substrate were sufficient to support specific cleavage.
The catalytic domain of numerous hammerhead ribozymes have now been studied by both the Uhlenbeck's and Symons' groups with regard to defining the nucleotides required for specific assembly and catalytic activity, and determining the rates of cleavage under various conditions.
Haseloff and Gerlach showed it was possible to divide the domains of the hammerhead ribozyme in a different manner. By doing so, they placed most of the required sequences in the strand that did not get cut (the ~ 095/06731 2 1 6 ~ ~ 4 5 PCT~S94/09342 ribozyme) and only a required UH where H = C, A, or U in the strand that did get cut (the substrate). This resulted in a catalytic ribozyme that could be designed to cleave any UH RNA sequence embedded within a longer "substrate recognition" sequence. The specific cleavage of a long mRNA, in a predictable manner using several such hammerhead ribozymes, was reported in 1988.
One plant pathogen RNA (from the negative strand of the tobacco ringspot virus) undergoes self-cleavage but cannot be folded into the consensus hammerhead structure described above. Bruening and colleagues have independently identified a 50-nucleotide catalytic domain for this RNA. In 1990, Hampel and Tritz succeeded in dividing the catalytic domain into two parts that could act as substrate and ribozyme in a multiple-turnover, cutting reaction. As with the hammerhead ribozyme, the catalytic portion contains most of the sequences required for catalytic activity, while only a short sequence (GUC
in this case) is required in the target. Hampel and Tritz described the folded structure of this RNA as consisting of a single hairpin and coined the term "hairpin" ribozyme (Bruening and colleagues use the term "paperclip" for this ribozyme motif). Continuing experiments suggest an increasing number of similarities between the hairpin and hammerhead ribozymes in respect to both binding of target RNA and mechanism of cleavage.
Hepatitis Delta Virus (HDV) is a virus whose genome consists of single-stranded RNA. A small region (about 80 nucleotides) in both the genomic RNA, and in the complementary anti-genomic RNA, is sufficient to support self-cleavage. In 1991, Been and Perrotta proposed a secondary structure for the HDV RNAs that is conserved between the genomic and anti-genomic RNAs and is necessary for catalytic activity. Separation of the HDV RNA into "ribozyme" and "substrate" portions has recently been achieved by Been. Been has also succeeded in reducing the size of the HDV ribozyme to about 60 nucleotides.
WO95/06731 PCT~S94/09342 ~16~
The table below lists some of the characteristics of the ribozymes discussed above:
Characteristics of Ribozymes Group I Introns Size: ~300 to >lO00 nucleotides.
Re~uires a U in the target sequence immediately 5' of the cleavage site.
Binds 4-6 nucleotides at 5' side of the cleavage site.
Over lO0 known members of this class. Found in Tetrahymena thermophila rRNA, fungal mitochondria, chloroplasts, phage T4, blue-green algae, and others.
RNaseP RN~ ~Ml RN~) Size: -290 to 400 nucleotides.
RNA portion of a ribonucleoprotein enzyme. Cleaves tRNA precursors to form mature tRNA.
Roughly lO known members of this group all are bacterial in origin.
Hammerhead Ribozyme Size: ~13 to 40 nucleotides.
Requires the target sequence UH immediately 5' of the cleavage site.
Binds a variable number nucleotides on both sides of the cleavage site.
14 known members of this class. Found in a number of plant pathogens (virusoids) that use RNA as the infectious agent. (Figure 1) Hairpin Ribozyme Size: -50 nucleotides.
Requires the target sequence GUC immediately 3' of the cleavage site.
Binds 4 nucleotides at 5' side of the cleavage site and a variable number to the 3' side of the cleavage site.
Only 1 known member of this class. Found in one plant pathogen (satellite RNA of the tobacco ringspot WO95/06731 2 ~ PCT~S94/09342 virus) which uses RNA as the infectious agent.
(Figure 2) Hepatitis Delta Virus (HDV) Ribozyme Size: ~60 nucleotides (at present).
Cleavage of target RNAs recently demonstrated.
Sequence requirements not fully determined.
Binding sites and structural requirements not fully determined, although no sequences 5' of cleavage site are required.
Only 1 known member of this class. Found in human HDV. (Figure 3) Eckstein et al., International Publication No.
WO 92/07065; Perrault et al., Nature 1990, 344:565; Pieken et al., Science 1991, 253:314; Usman and Cedergren, Trends in Biochem. sci. 1992, 17:334; Usman et al ., International Publication No. Wo 93/15187; and Rossi et al., International Publication No. WO 91/03162, describe various chemical modifications that can be made to the sugar moieties of enzymatic nucleic acid molecules.
Summarv of the Invention This invention concerns the use of non-nucleotide molecules as spacer elements at the base of double-stranded nucleic acid (e . g., RNA or DNA) stems (duplex stems) or in the single-stranded regions, catalytic core, loops, or recognition arms of enzymatic nucleic acids. Duplex stems are ubiquitous structural elements in enzymatic RNA molecules. To facilitate the synthesis of such stems, which are usually connected via single-stranded nucleotide chains, a base or base-pair mimetic may be used to reduce the nucleotide requirement in the synthesis of such molecules, and to confer nuclease resistance (since they are non-nucleic acid components).
This also applies to both the catalytic core and recognition arms of a ribozyme.
Examples of such non-nucleotide mimetics are shown in Figure 4 and their incorporation into hammerhead ~:L69~
WO95/06731 PCT~S94/09342 ribozymes is shown in Figure 5. These non-nucleotide linkers may be either polyether, polyamine, polyamide, or polyhydrocarbon compounds. Specific examples include those described by Seela and Kaiser, Nucleic Acids Res.
1990, 18:6353 and Nucleic Acids Res. 1987, 15:3113; Cload and Schepartz, J. Am. Chem. Soc. 1991, 113:6324;
Richardson and Schepartz, J. Am. Chem. Soc. 1991, 113 : 5109 ; Ma et al., Nucleic Acids Res. 1993, 21 : 2585 and Biochemistry 1993, 32: 1751; Durand et al., Nucleic Acids Res. l99O,18: 6353; McCurdy et al., Nucleosides &
Nucleotides 1991, lo : Z87 ; Jaschke et al., Tetrahedron Lett. 1993, 34:301; Ono et al., Biochemistry 1991, 30 : 9914 ; Arnold et al., International Publication No.
Wo 89/02439 entitled "Non-nucleotide Linking Reagents for 15 Nucleotide Probes"; and Ferentz and Verdine, ~ . Chem.
Soc. 1991, 113: 4000, all hereby incorporated by reference herein.
Thus, in a first aspect, the invention features an enzymatic nucleic acid molecule having one or more 20 non-nucleotide moieties, and having enzymatic activity to cleave an RNA or DNA molecule.
Examples of such non-nucleotide mimetics are shown in Figure 4 and their incorporation into hammerhead ribozymes is shown in Figure 5. These non-nucleotide linkers may be either polyether, polyamine, polyamide, or polyhydrocarbon compounds.
In preferred embodiments, the enzymatic nucleic acid includes one or more stretches of RNA, which provide the enzymatic activity of the molecule, linked to the non-nucleotide moiety.
By the term "non-nucleotide" is meant any group or compound which can be incorporated into a nucleic acid chain in the place of one or more nucleotide units, including either sugar and/or phosphate substitutions, and allows the remaining bases to exhibit their enzymatic activity. The group or compound is abasic in that it does not contain a commonly recognized nucleotide base, such as ~WO95/06731 2 ~ 6 ~ ~ ~ S PCT~S94/09342 adenosine, guanine, cytosine, uracil or thymine. It may have substitutions for a 2' or 3' H or OH as described in the art. See Eckstein et al. and Usman et al., supra.
In preferred embodiments, the enzymatic nucleic acid includes one or more stretches of RNA, which provide the enzymatic activity of the molecule, linked to the non-nucleotide moiety. The necessary RNA components are known in the art, see, e.g., Usman, sup~a.
As the term is used in this application, non-nucleotide-containing enzymatic nucleic acid means a nucleic acid molecule that contains at least one non-nucleotide component which replaces a portion of a ribozyme, e.g., but not limited to, a double-stranded stem, a single-stranded "catalytic core" sequence, a single-stranded loop or a single-stranded recognition sequence. These molecules are able to cleave (preferably, repeatedly cleave) separate RNA or DNA molecules in a nucleotide base sequence specific manner. Such molecules can also act to cleave intramolecularly if that is desired. Such enzymatic molecules can be targeted to virtually any RNA transcript. Such molecules also include nucleic acid molecules having a 3' or 5' non-nucleotide, useful as a capping group to prevent exonuclease digestion.
Enzymatic molecules of this invention act by first binding to a target RNA or DNA. Such binding occurs through the target binding portion of the enzyme which is held in close proximity to an enzymatic portion of molecule that acts to cleave the target RNA or DNA. Thus, the molecule first recognizes and then binds a target nucleic acid through complementary base-pairing, and once bound to the correct site, acts enzymatically to cut the target. Strategic cleavage of such a target will destroy its ability to direct synthesis of an encoded protein.
After an enzyme of this invention has bound and cleaved its target it is released from that target to search for W O 95/06731 . PCTrUS94/09342 9 ~
another target, and can repeatedly bind and cleave new targets.
The enzymatic nature of an enzyme of this invention is advantageous over other technologies, such as antisense technology (where a nucleic acid molecule simply binds to a nucleic acid target to block its translation) since the effective concentration of the enzyme necessary to effect a therapeutic treatment is lower than that of an antisense oligonucleotide. This advantage reflects the ability of the enzyme to act enzymatically. Thus, a single enzyme molecule is able to cleave many molecules of target RNA. In addition, the enzyme is a highly specific inhibitor, with the specificity of inhibition depending not only on the base pairing mechanism of binding, but also on the mechanism by which the molecule inhibits the expression of the RNA to which it binds. That is, the inhibition is caused by cleavage of the target and so specificity is defined as the ratio of the rate of cleavage of the targeted nucleic acid over the rate of cleavage of non-targeted nucleic acid. This cleavage mechanism is dependent upon factors additional to those involved in base pairing. Thus, it is thought that the specificity of action of an enzyme of this invention is greater than that of antisense oligonucleotide binding the same target site.
By the phrase enzyme is meant a catalytic non-nucleotide-containing nucleic acid molecule that has complementarity in a substrate-binding region to a specified nucleic acid target, and also has an enzymatic activity that specifically cleaves RNA or DNA in that target. That is, the enzyme is able to intramolecularly or intermolecularly cleave RNA or DNA and thereby inactivate a target RNA or DNA molecule. This complementarity functions to allow sufficient hybridization of the enzymatic molecule to the target RNA
or DNA to allow the cleavage to occur. One hundred 095/06731 ~ 6 4 5 PCT~S94/09342 percent complementarity is preferred, but complementarity as low as 50-75~ may also be useful in this invention.
In preferred embodiments of this invention, the enzyme molecule is formed generally in a hammerhead motif, but may also be formed in the motif of a hairpin, hepatitis delta virus, group I intron or RNaseP RNA (in association with an RNA guide sequence). Examples of such hammerhead motifs are described by Rossi et al., Aids Research and Human Retroviruses 1992, 8:183; of hairpin motifs by Hampel et al., "RNA Catalyst for Cleaving Specific RNA Sequences," filed September 20, 1989, which is a continuation-in-part of U.S. Serial No. 07/247,100 filed September 20, 1988, Hampel and Tritz, Biochemistry 1989, 28:4929, and Hampel et al ., Nucleic Acids Research l99o, 18:299; and an example of the hepatitis delta virus motif is described by Perrotta and Been, Biochemistry 1992, 31:16; of the RNaseP motif by Guerrier-Takada et al ., Cell 1983, 35:849; and of the Group I intron by Cech et al , U. S. Patent 4,987,071. These specific motifs are not limiting in the invention and those skilled in the art will recognize that all that is important in an enzyme molecule of this invention is that it have at least one non-nucleotide portion, and a specific substrate-binding site which is complementary to one or more of the target gene RNA regions, and that it have nucleotide sequences within or surrounding that substrate-binding site which impart a nucleic acid cleaving activity to the molecule.
The invention provides a method for producing a class of enzymatic cleaving agents which exhibit a high degree of specificity for the nucleic acid of a desired target. The enzyme molecule is preferably targeted to a highly conserved sequence region of a target such that specific treatment of a disease or condition can be provided with a single enzyme. Such enzyme molecules can be delivered exogenously to specific cells as required.
In the preferred hammerhead motif the small size (less than 60 nucleotides, preferably between 30-40 nucleotides WO95/06731 ~ PCT~S94/09342 in length) of the molecule allows the cost of treatment to be reduced compared to other ribozyme motifs.
Synthesis of nucleic acids greater than lOO
nucleotides in length is difficult using automated methods, and the therapeutic cost of such molecules is prohibitive. In this invention, small enzyme motifs (e.g., of the hammerhead structure) are used for exogenous delivery. The simple structure of these molecules increases the ability of the enzyme to invade targeted regions of mRNA structure. Unlike the situation when the hammerhead structure is included within longer transcripts, there are no non-enzyme flanking sequences to interfere with correct folding of the enzyme structure or with complementary regions.
Other features and advantages of the invention will be apparent from the following description of the preferred embodiments thereof, and from the claims.
Descri~tion of the Preferred Embodiments The drawings will first briefly be described.
ZO Drawinqs:
Figure l is a diagrammatic representation of the hammerhead ribozyme domain known in the art.
Figure 2 is a diagrammatic representation of the general structure of the hairpin ribozyme domain known in the art.
Figure 3 is a diagrammatic representation of the general structure of the hepatitis delta virus ribozyme domain known in the art.
Figure 4 is a diagrammatic representation of various non-nucleotide mimetics that may be incorporated into nucleic acid enzymes. Standard abbreviations are used in the Figure. In compound l each X may independently be oxygen, nitrogen, sulfur or substituted carbons containing alkyl, alkene or equivalent chains of length l-lO carbon atoms. In compounds 6, 6a, 7, 8, 9 and lO each Y may independently be a phosphodiester, ether or amide linkage to the rest of the nucleic acid enzyme. In 095/06731 2 1 6 ~ PCT~S94/09342 compounds 4 and 5 each R may independently be H, OH, protected OH, O-alkyl, alkenyl or alkynyl or alkyl, alkenyl or alkynyl of l-lO carbon atoms.
Figure 5 is a diagrammatic representation of the preferred location for incorporation of various non-nucleotide mimetics into nucleic acid enzymes.
Specifically, mimetics, l-lO, may replace the loop (denoted as / / in Figure 5) that connects the two strands of Stem II. Stem II itself may be from l to lO
base pairs. In examples l ~ 2 below compounds l and 2 were incorporated into molecules having a stem II of l to 5 basepairs in length. Compounds l, 4 and 5 may also replace nucleotides in the recognition arms of stems I and III or in stem II itself.
Figure 6 is a diagrammatic representation of the synthesis of a perylene based non-nucleotide mimetic phosphoramidite 3.
Figure 7 is a diagrammatic representation of the synthesis of an abasic deoxyribose or ribose non-nucleotide mimetic phosphoramidite.
Figures 8a and 8b are graphical representations of cleavage of substrate by various ribozymes at 8nM, or 40 nM, respectively.
Non-nucleotide Mimetics Non-nucleotide mimetics useful in this invention are generally described above. Those in the art will recognize that these mimetics can be incorporated into an enzymatic molecule by standard techniques at any desired location. Suitable choices can be made by standard experiments to determine the best location, e.g., by synthesis of the molecule and testing of its enzymatic activity. The optimum molecule will contain the known ribonucleotides needed for enzymatic activity, and will have non-nucleotides which change the structure of the molecule in the least way possible. What is desired is that several nucleotides can be substituted by one non-nucleotide to save synthetic steps in enzymatic molecule ~169~5 . `
WO95/06731 PCT~S94/09342 synthesis and to provide enhanced stability of the molecule compared to RNA or even DNA.
Examples The following are non-limiting examples showing the synthesis of non-nucleotide mimetic-containing catalytic nucleic acids using non-nucleotide phosphoramidites.
Example 1: SYnthesis of Hammerhead Ribozymes Containinq Non-nucleotide Mimetics: PolYether SPaCers Polyether spacers, compound 1 (Figure 4; X-O, n=2 or 4), have been incorporated both singly, n=2 or 4, or doubly, n=2, at the base of stem II of a hammerhead ribozyme, replacing loop 2, and shown to produce a ribozyme which has lower catalytic efficiency. The method of synthesis used followed the procedure for normal RNA
synthesis as described in Usman et al., J. Am. Chem. Soc.
1987, ld9:7845 and in Scaringe et al., Nucleic Acids ~es .
1990, 18:5433, and makes use of common nucleic acid protecting and coupling groups, such as dimethoxytrityl at the 5'-end, and phosphoramidites at the 3'-end. The average stepwise coupling yields were >98%. The design of these types of mimetics has not been optimized to date, but, as discussed above, this can be readily achieved using standard experimental techniques. These experiments indicate the potential of such mimetics to replace the loops and portions of stems in ribozymes while maintaining catalytic activity. These mimetics may be incorporated not only into hammerhead ribozymes, but also into hairpin, hepatitis delta virus, or Group 1 or Group 2 introns.
They are, therefore, of general use as replacement motifs in any nucleic acid structure. Use of such mimetics allows about 2-10 nucleotides to be omitted from the final nucleic acid molecule compared to the use of an oligonucleotide without a non-nucleotide mimetic.
ExamPle 2: SYnthesis of Hammerhead RibozYmes Containinq Non-nucleotide Mimetics: Aromatic SPacers ~WO95/06731 2 ~ 3 PCT~S94/09342 -In another example, a specific linker for the base of the stem II C-G of a hammerhead ribozyme was designed. Applicant believes that the distance between the C1' carbons of the C-G base pair is about 16 Angstroms. To join these two pieces of RNA by a covalent analog of the C-G base pair a new type of dimer phosphoramidite containing a linker between the 3'-OH and the 5'-OH of the G and C residues respectively can be constructed. Two types of base-pair mimetic are the rigid aromatic spacers, 2 or 3, shown in Figure 4. These have been incorporated at the base of stem II of a hammerhead ribozyme as described in Example 1, replacing loop 2, and shown to produce a ribozyme which has lower catalytic efficiency. Another mimetic is a flexible alkyl spacer similar to the polyamide backbone described by Nielsen et al., Science 1991, 254:1497 (see, Figure 4; 6 or a derivative thereof 6a; Zuckerman et al., J. Am. Chem. Soc.
1992, 114:10464). Use of such mimetics allows about 2-10 nucleotides to be omitted from the final nucleic acid molecule compared to the use of an oligonucleotide without a non-nucleotide mimetic.
ExamPle 3: SYnthesis of Non-nucleotide Mimetics Aromatic Spacer Phosphoramidite 2 This compound was originally described by Salunkhe et al., J. Am. Chem. Soc. 1992, 114:6324. The synthesis was modified as follows: To terphthalic acid (1.0 g, 6.0 mmol) in DMF (12 mL) was added EDC (2.54 g, 13.2 mmol), aminohexanol (1.55 g, 13.2 mmol) and N-methylmorpholine (1.45 mL, 13.2 mmol). The reaction mixture was stirred overnight at which time the solution was cloudy. Water was added to the reaction mixture to precipitate out the product. The solid was filtered and washed with water and dried to provide 562 mg (25.7~) of the diol.
To the diol (250 mg, 0.687 mmol) in DMSO (40 mL) was added triethylamine (287 ~L, 2.06 mmol), dimethoxytrityl chloride (220 mg, 0.653 mmol) and WO95/06731 PCT~S94/09342 -catalytic DMAP. The reaction mixture was heated to 40C
and stirred overnight. The mixture was then cooled to room temperature (about 20-25C), quenched with water and extracted three times with EtOAc. A solid precipitate remained in the organic layer that was isolated and found to be starting diol (50 mg, 20~). The organic layer was dried over Na2SO4 and evaporated. The resulting oil was purified with flash chromatography (10% EtOAc in hexanes to 100% EtOAc) to yield 250 mg (55%) of the monotritylated compound.
To the alcohol (193 mg, 0.29 mmol) in THF (1 mL) at 0C was added diisopropylethylamine (101 ~L, 0.58 mmol)~
and then 2-cyanoethyl N,N-diisopropylamino chlorophosphoramidite (78 ~L, 0.35 mmol) dropwise. The resulting mixture was stirred for 5 minutes and then warmed to room temperature. After 1 hour the reaction mixture was quenched with methanol and evaporated. The resulting oil was purified by flash chromatography (1:1 hexanes:EtOAc) to yield 158 mg (63%) of the phosphoramidite.
ExamPle 4: SYnthesis of Non-nucleotide Mimetics Aromatic Spacer Phosphoramidite 3 Referring to Figure 6, to 3, 4, 9, 10-perylenetetracarboxylic dianhydride 11 (1.0 g, 2.55 mmol) in quinoline (10 mL) was added ethanolamine (919 ~L, 15.3 mmol) and ZnOAc-2.5 H2O (140 mg, 0.638 mmol). The reaction mixture was heated to 190C for 8 hours. The solution was then cooled, lN HCl added to precipitate the product and the mixture was filtered. The solid was washed with hot 10% CaCO3 until the filtrate was no longer pale green. The remaining bright red precipitate 12 was then dried.
The resulting diol 12 was then treated as outlined above for 2 to provide the phosphoramidite 3.
ExamPle 5: SYnthesis of Hammerhead Ribozymes Containinq Non-nucleotide Mimetics: Abasic Nucleotides 4 and s ~ 095/06731 2 1 6 9 6 4 5 PCT~S94/09342 Compound 4, R=H, was prepared according to Iyer et alO ~ Nucleic Acids Res. 1990, 18:2855. Referring to Figure 7, compounds 4 and 5 (R=O-t-butyldimethylsilyl) phosphoramidites were prepared as follows:
To a solution of D-ribose (20.0 g, 0.105 mol) in N,N-dimethylformamide (250 mL) was added 2,2-dimethoxypropane (50 mL) and p-toluenesulfonic acid monohydrate (300 mg). The reaction mixture was stirred for 16 hours at room temperature and then evaporated to dryness. The crude product was coevaporated with pyridine (2 x 150 mL), dissolved in dry pyridine (300 mL) and 4,4'-dimethoxytrityl chloride (37.2 g, 0.110 mol) was added and stirred for 24 hours at room temperature. The reaction mixture was diluted with methanol (50 mL) and evaporated to dryness. The residue was dissolved in chloroform (800 mL) and washed with 5% NaHC03 (2 x 200 mL), brine (300 mL), dried, evaporated, coevaporated with toluene (2 x 100 mL) and purified by flash chromatography in CHCI3to yield 40.7 g (78.1%) of compound a.
To a solution of dimethoxytrityl derivative a (9.o g, 18.3 mmol) and DMAP (4.34 g, 36 mmol) in dry CH3CN, phenoxythiocarbonyl chloride (3.47 g, 20.1 mmol) was added dropwise under argon. The reaction mixture was left for 16 hours at room temperature, then evaporated to dryness.
The resulting residue was dissolved in chloroform (200 mL), washed with 5% NaHCO3, brine, dried, evaporated and purified by flash chromatography in CHCI3, to yield 8.0 g (69.5%) of compound b as the ~-anomer.
To a solution of intermediate b (3.0 g, 4.77 mmol) in toluene (50 mL) was added AIBN (0.82 g, 5.0 mmol) and Bu3SnH (1.74 g, 6.0 mmol) under argon and the reaction - mixture was kept at 80C for 7 hours. The solution was evaporated and the resulting residue purified by flash - chromatography in CHCl3 to yield 1.5 g (66%) of protected ribitol c.
Subsequent removal of all protecting groups by acid treatment and tritylation provided the protected WO95/06731 ~ PCT~S94/09342 ribitol d which was then converted to target phosphoramidites 4 and 5 by the general method described in Scaringe et al., Nucleic Acids Res . 1990, 18: 5433 .
ExamPle 6 Referring to Figures 8a and 8b the cleavage of substrate is shown by various modified ribozymes compared to unmodified ribozyme at 8nM and 40nM concentrations.
Specifically, a control ribozyme of sequence ucuccA UCU
GAU GAG GCC GAA AGG CCG AAA Auc ccU (where lower case includes a 2' O-methyl group) was compared to ribozyme A
(ucu ccA UCU GAU GAG GCC SGG CCG AAA Auc ccu), B (ucu ccA
UCU GAU GAG CSG CG AAA Auc ccu), C (ucu ccA UCU GAU GAG
GCC bbb bGG CCG AAA Auc ccu), and D (ucu ccA UCU GAU GAG
Cbb bbG CGAA AAu ccc u) (where S=hexaethylene glycol linker); and b=abasic nucleotide 4). All were active in cleaving substrate.
Administration of RibozYme Selected ribozymes can be administered prophylactically, to viral infected patients or to diseased patients, e.g., by exogenous delivery of the ribozyme to a relevant tissue by means of an appropriate delivery vehicle, e.g., a liposome, a controlled release vehicle, by use of iontophoresis, electroporation or ion paired molecules, or covalently attached adducts, and other pharmacologically approved methods of delivery.
Routes of administration include intramuscular, aerosol, oral (tablet or pill form), topical, systemic, ocular, intraperitoneal and/or intrathecal.
The specific delivery route of any selected ribozyme will depend on the use of the ribozyme.
Generally, a specific delivery program for each ribozyme will focus on unmodified ribozyme uptake with regard to intracellular localization, followed by demonstration of efficacy. Alternatively, delivery to these same cells in an organ or tissue of an animal can be pursued. Uptake studies will include uptake assays to evaluate cellular ribozyme uptake, regardless of the delivery vehicle or ~ 095/06731 216 ~ ~ ~ 5 PCT~S94/09342 , strategy. Such assays will also determine the intracellular localization of the ribozyme following uptake, ultimately establishing the requirements for maintenance of steady-state concentrations within the 5 cellular compartment containing the target sequence (nucleus and/or cytoplasm). Efficacy and cytotoxicity can then be tested. Toxicity will not only include cell viability but also cell function.
Some methods of delivery that may be used 10 include:
a. encapsulation in liposomes, b. transduction by retroviral vectors, c. conjugation with cholesterol, d. localization to nuclear compartment utilizing antigen binding or nuclear targeting site found on most snRNAs or nuclear proteins, e. neutralization of charge of ribozyme by using nucleotide derivatives, and f. use of blood stem cells to distribute ribozymes throughout the body.
Delivery strategies useful in the present invention, include: ribozyme modifications, and particle carrier drug delivery vehicles. Unmodified ribozymes, like most small molecules, are taken up by cells, albeit slowly. To enhance cellular uptake, the ribozyme may be modified essentially at random, in ways which reduce its charge but maintains specific functional groups. This results in a molecule which is able to diffuse across the cell membrane, thus removing the permeability barrier.
Modification of ribozymes to reduce charge is just one approach to enhance the cellular uptake of these larger molecules. The random approach, however, is not advisable since ribozymes are structurally and functionally more complex than small drug molecules. The structural requirements necessary to maintain ribozyme catalytic activity are well understood by those in the W095/06731 ~ PCT~S94/09342 æ~9~
art. These requirements are taken into consideration when designing modifications to enhance cellular delivery. The modifications are also designed to reduce susceptibility to nuclease degradation. Both of these characteristics should greatly improve the efficacy of the ribozyme.
Cellular uptake can be increased by several orders of magnitude without having to alter the phosphodiester linkages necessary for ribozyme cleavage activity.
Chemical modifications of the phosphate backbone will reduce the negative charge allowing free diffusion across the membrane. This principle has been successfully demonstrated for antisense DNA technology. The similarities in chemical composition between DNA and RNA
make this a feasible approach. In the body, maintenance of an external concentration will be necessary to drive the diffusion of the modified ribozyme into the cells of the tissue. Administration routes which allow the diseased tissue to be exposed to a transient high concentration of the drug, which is slowly dissipated by systemic adsorption are preferred. Intravenous administration with a drug carrier designed to increase the circulation half-life of the ribozyme can be used.
The size and composition of the drug carrier restricts rapid clearance from the blood stream. The carrier, made to accumulate at the site of infection, can protect the ribozyme from degradative processes.
Drug delivery vehicles are effective for both systemic and topical administration. They can be designed to serve as a slow release reservoir, or to deliver their contents directly to the target cell. An advantage of using direct delivery drug vehicles is that multiple molecules are delivered per uptake. Such vehicles have been shown to increase the circulation half-life of drugs which would otherwise be rapidly cleared from the blood stream. Some examples of such specialized drug delivery vehicles which fall into this category are liposomes, ~WO95/06731 21~ PCT~S94109342 hydrogels, cyclodextrins, biodegradable nanocapsules, and bioadhesive microspheres.
From this category of delivery systems, liposomes are preferred. Liposomes increase intracellular stability, increase uptake efficiency and improve biological activity.
Liposomes are hollow spherical vesicles composed of lipids arranged in a similar fashion as those lipids which make up the cell membrane. They have an internal aqueous space for entrapping water soluble compounds and range in size from 0.05 to several microns in diameter.
Several studies have shown that liposomes can deliver RNA
to cells and that the RNA remains biologically active.
For example, a liposome delivery vehicle originally designed as a research tool, Lipofectin, has been shown to deliver intact mRNA molecules to cells yielding production of the corresponding protein. In another study, an antibody targeted liposome delivery system containing an RNA molecule 3,500 nucleotides in length and antisense to a structural protein of HIV, inhibited virus proliferation in a sequence specific manner. Not only did the antibody target the liposomes to the infected cells, but it also triggered the internalization of the liposomes by the infected cells.
Triggering the endocytosis is useful for viral inhibition.
Finally, liposome delivered synthetic ribozymes have been shown to concentrate in the nucleus of H9 (an example of an HIV-sensitive cell) cells and are functional as evidenced by their intracellular cleavage of the sequence.
Liposome delivery to other cell types using smaller ribozymes (less than 142 nucleotides in length) exhibit different intracellular localizations.
Liposomes offer several advantages: They are non-toxic and biodegradable in composition; they display long circulation half-lives; and recognition molecules can be readily attached to their surface for targeting to tissues. Finally, cost effective manufacture of liposome-WO95/06731 PCT~S94109342 based pharmaceuticals, either in a liquid suspension orlyophilized product, has demonstrated the viability of this technology as an acceptable drug delivery system.
Other controlled release drug delivery systems, such as nonoparticles and hydrogels may be potential delivery vehicles for a ribozyme. These carriers have been developed for chemotherapeutic agents and protein-based pharmaceuticals, and consequently, can be adapted for ribozyme delivery.
Topical administration of ribozymes is advantageous since it allows localized concentration at the site of administration with minimal systemic adsorption. This simplifies the delivery strategy of the ribozyme to the disease site and reduces the extent of toxicological characterization. Furthermore, the amount of material to be applied is far less than that required for other administration routes. Effective delivery requires the ribozyme to diffuse into the infected cells.
Chemical modification of the ribozyme to neutralize negative charge may be all that is required for penetration. However, in the event that charge neutralization is insufficient, the modified ribozyme can be co-formulated with permeability enhancers, such as Azone or oleic acid, in a liposome. The liposomes can either represent a slow release presentation vehicle in which the modified ribozyme and permeability enhancer transfer from the liposome into the infected cell, or the liposome phospholipids can participate directly with the modified ribozyme and permeability enhancer in facilitating cellular delivery. In some cases, both the ribozyme and permeability enhancer can be formulated into a suppository formulation for slow release.
Ribozymes may also be systemically administered.
Systemic absorption refers to the accumulation of drugs in the blood stream followed by distribution throughout the entire body. Administration routes which lead to systemic absorption include: intravenous, subcutaneous, ~ WO95/06731 2 1~ 9 ~ I ~ PCT~S94/09342 .
intraperitoneal, intranasal, intrathecal and ophthalmic.
Each of these administration routes expose the ribozyme to an accessible diseased tissue. Subcutaneous administration drains into a localized lymph node which proceeds through the lymphatic network into the circulation. The rate of entry into the circulation has been shown to be a function of molecular weight or size.
The use of a liposome or other drug carrier localizes the ribozyme at the lymph node. The ribozyme can be modified to diffuse into the cell, or the liposome can directly participate in the delivery of either the unmodified or modified ribozyme to the cell. This method is particularly useful for treating AIDS using anti-HIV
ribozymes.
Also preferred in AIDS therapy is the use of a liposome formulation which can deliver oligonucleotides to lymphocytes and macrophages. This oligonucleotide delivery system inhibits HIV proliferation in infected primary immune cells. Whole blood studies show that the formulation is taken up by 90~ of the lymphocytes after 8 hours at 37C. Preliminary biodistribution and pharmacokinetic studies yielded 70% of the injected dose/gm of tissue in the spLeen after one hour following intravenous administration. This formulation offers an excellent delivery vehicle for anti-AIDS ribozymes for two reasons. First, T-helper lymphocytes and macrophages are the primary cells infected by the virus, and second, a subcutaneous administration delivers the ribozymes to the resident HIV-infected lymphocytes and macrophages in the lymph node. The liposomes then exit the lymphatic system, enter the circulation, and accumulate in the spleen, where the ribozyme is delivered to the resident lymphocytes and macrophages.
Intraperitoneal administration also leads to entry into the circulation, with once again, the molecular weight or size of the ribozyme-delivery vehicle complex controlling the rate of entry.
WO95/06731 ; PCT~S94/093~2 ~
4 ~
Liposomes injected intravenously show accumulation in the liver, lung and spleen. The composition and size can be adjusted so that this accumulation represents 30% to 40% of the injected dose.
The remaining dose circulates in the blood stream for up to 24 hours.
The chosen method of delivery should result in cytoplasmic accumulation in the afflicted cells and molecules should have some nuclease-resistance for optimal dosing. Nuclear delivery may be used but is less preferable. Most preferred delivery methods include liposomes (10-400 nm), hydrogels, controlled-release polymers, microinjection or electroporation (for ex vivo treatments) and other pharmaceutically applicable vehicles. The dosage will depend upon the disease indication and the route of administration but should be between 100-200 mg/kg of body weight/day. The duration of treatment will extend through the course of the disease symptoms, usually at least 14-16 days and possibly continuously. Multiple daily doses are anticipated for topical applications, ocular applications and vaginal applications. The number of doses will depend upon disease delivery vehicle and efficacy data from clinical trials.
Establishment of therapeutic levels of ribozyme within the cell is dependent upon the rate of uptake and degradation. Decreasing the degree of degradation will prolong the intracellular half-life of the ribozyme.
Thus, chemically modified ribozymes, e.g., with modification of the phosphate backbone, or capping of the 5' and 3' ends of the ribozyme with nucleotide analogues may require different dosaging. Descriptions of useful systems are provided in the art cited above, all of which is hereby incorporated by reference herein.
For a more detailed description of ribozyme design, see, Draper, U.S. Serial No. 08/103,243 filed 095/06731 216 9 ~ 4 5 PCT~S94/09342 August 6, 1993, hereby incorporated by reference herein in its entirety.
Other embodiments are within the following claims.
~ ,i l., WO95/06731 PCT~S94/09342 ~
~69~5 "Sequence Listing"
(1) GENERAL INFORMATION:
(i) APPLICANT: Nassim Usman Francine E. Wincott Jasenka Matulic-Adamic Leonid Beigelman (ii) TITLE OF INVENTION: NON-NUCLEOTIDE CONTAINING
ENZYMATIC NUCLEIC ACID
(iii) NUMBER OF SEQUENCES: 5 (iv) CORRESPONDENCE ADDRESS:
(A) ADDRESSEE: Lyon & Lyon (B) STREET: 611 West Sixth Street (C) CITY: Los Angeles (D) STATE: California (E) COUNTRY: USA
(F) ZIP: 90017 (v) CO~ .~ READABLE FORM:
(A) MEDIUM TYPE: 3.5" Diskette, 1.44 Mb storage (B) COMPUTER: IBM Compatible (C) OPERATING SYSTEM: IBM P.C. DOS (Version 5.0) (D) SOFTWARE: WordPerfect (Version 5.1) (vi) CURRENT APPLICATION DATA:
(A) APPLICATION NUMBER:
(B) FILING DATE:
(C) CLASSIFICATION:
(vii) PRIOR APPLICATION DATA:
Prior applications total, including application described below: two (A) APPLICATION NUMBER: 08/152,488 (B) FILING DATE: 12 NOV 1993 (A) APPLICATION NUMBER: 08/116,177 (B) FILING DATE: 02 SEPT 1993 (viii) ATTORNEY/AGENT INFORMATION:
(A) NAME: . Warburg, Richard J.
(B) REGISTRATION NUMBER: 32,327 (C) REFERENCE/DOCKET NUMBER: 206/267 ~ WO95/06731 ~ 1 ~t9'l~ 4 ~ PCT~S94/09342 , . . . .
(ix) TELECOMMUNICATION INFORMATION:
(A) TELEPHONE: (213) 489-1600 (B) TELEFAX: (213) 955-0440 (C) TELEX: 67-3510 (2) INFORMATION FOR SEQ ID NO: 1:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 11 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base. "H"
r e p r e s e n t s nucleotide C, A, or U.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 1:
(2) INFORMATION FOR SEQ ID NO: 2:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 46X (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 2:
WO95106731 ;~ PCT~S94/09342 ~
~169~4~
(2) INFORMATION FOR SEQ ID NO: 3:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: l4 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 3:
NNNNNGUCNN NNNN l4 (2) INFORMATION FOR SEQ ID NO: 4:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 50 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ix) FEATURE:
(D) OTHER INFORMATION: The letter "N"
stands for any base.
(ii) SEQUENCE DESCRIPTION: SEQ ID NO: 4:
(2) INFORMATION FOR SEQ ID NO: 5:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 85 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear ~ 095/06731 2 1 ~ ~ 6 ~ 5 PCT~S94/09342 (ii) SEQUENCE DESCRIPTION: SEQ ID NO: 5:
(2) INFORMATION FOR SEQ ID NO: 6:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 36 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) SEQUENCE DESCRIPTION: SEQ ID NO: 6:
(2) INFORMATION FOR SEQ ID NO: 7:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 33 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) SEQUENCE DESCRIPTION: SEQ ID NO: 7:
(2) INFORMATION FOR SEQ ID NO: 8:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 29 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) SEQUENCE DESCRIPTION: SEQ ID NO: 8:
(2) INFORMATION FOR SEQ ID NO: 9:
(i) SEQUENCE CHARACTERISTICS:
WO95/06731 PCT~S94/09342 ~
(A) LENGTH: 36 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) SEQUENCE DESCRIPTION: SEQ ID NO: 9:
(2) INFORMATION FOR SEQ ID NO: l0:
(i) SEQUENCE CHARACTERISTICS:
(A) LENGTH: 32 (B) TYPE: nucleic acid (C) STRANDEDNESS: single (D) TOPOLOGY: linear (ii) SEQUENCE DESCRIPTION: SEQ ID NO: l0:
Claims (3)
1. Enzymatic nucleic acid comprising a non-nucleotide.
2. The enzymatic nucleic acid of claim 1, wherein said non-nucleotide is provided in a double-stranded stem region, the catalytic core, or in a single-stranded recognition region.
3. The enzymatic nucleic acid of claim 1, wherein said non-nucleotide is selected from the group consisting of compound 1, 2, 3, 4 and 5.
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11617793A | 1993-09-02 | 1993-09-02 | |
US08/116,177 | 1993-09-02 | ||
US15248193A | 1993-11-12 | 1993-11-12 | |
US08/152,481 | 1993-11-12 | ||
US23374894A | 1994-04-19 | 1994-04-19 | |
US08/233,748 | 1994-04-19 |
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Publication Number | Publication Date |
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CA2169645A1 true CA2169645A1 (en) | 1995-03-09 |
Family
ID=27381788
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002169645A Abandoned CA2169645A1 (en) | 1993-09-02 | 1994-08-19 | Non-nucleotide containing enzymatic nucleic acid |
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US (5) | US5891683A (en) |
EP (3) | EP1602725A3 (en) |
JP (2) | JPH09502092A (en) |
AT (1) | ATE227342T1 (en) |
CA (1) | CA2169645A1 (en) |
DE (1) | DE69431669T2 (en) |
DK (1) | DK0748382T3 (en) |
ES (1) | ES2186690T3 (en) |
PT (1) | PT748382E (en) |
WO (1) | WO1995006731A2 (en) |
Families Citing this family (308)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6399754B1 (en) * | 1991-12-24 | 2002-06-04 | Isis Pharmaceuticals, Inc. | Sugar modified oligonucleotides |
JPH09502092A (en) | 1993-09-02 | 1997-03-04 | リボザイム・ファーマシューティカルズ・インコーポレイテッド | Enzymatic nucleic acid containing non-nucleotide |
EP0728204A1 (en) | 1993-11-08 | 1996-08-28 | Ribozyme Pharmaceuticals, Inc. | Base-modified enzymatic nucleic acid |
US5672501A (en) * | 1994-12-23 | 1997-09-30 | Ribozyme Pharmaceuticals, Inc. | Base-modified enzymatic nucleic acid |
US7034009B2 (en) | 1995-10-26 | 2006-04-25 | Sirna Therapeutics, Inc. | Enzymatic nucleic acid-mediated treatment of ocular diseases or conditions related to levels of vascular endothelial growth factor receptor (VEGF-R) |
US5728818A (en) * | 1996-01-16 | 1998-03-17 | Ribozyme Pharmaceuticals, Inc. | Chemical linkage of ribozyme protions |
US5998203A (en) * | 1996-04-16 | 1999-12-07 | Ribozyme Pharmaceuticals, Inc. | Enzymatic nucleic acids containing 5'-and/or 3'-cap structures |
US20040171030A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Oligomeric compounds having modified bases for binding to cytosine and uracil or thymine and their use in gene modulation |
US20040203024A1 (en) * | 1996-06-06 | 2004-10-14 | Baker Brenda F. | Modified oligonucleotides for use in RNA interference |
US20040171028A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Phosphorous-linked oligomeric compounds and their use in gene modulation |
US20040147022A1 (en) * | 1996-06-06 | 2004-07-29 | Baker Brenda F. | 2'-methoxy substituted oligomeric compounds and compositions for use in gene modulations |
US20030044941A1 (en) * | 1996-06-06 | 2003-03-06 | Crooke Stanley T. | Human RNase III and compositions and uses thereof |
US5898031A (en) * | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
US20040266706A1 (en) * | 2002-11-05 | 2004-12-30 | Muthiah Manoharan | Cross-linked oligomeric compounds and their use in gene modulation |
US20050119470A1 (en) * | 1996-06-06 | 2005-06-02 | Muthiah Manoharan | Conjugated oligomeric compounds and their use in gene modulation |
US9096636B2 (en) * | 1996-06-06 | 2015-08-04 | Isis Pharmaceuticals, Inc. | Chimeric oligomeric compounds and their use in gene modulation |
US20040171031A1 (en) * | 1996-06-06 | 2004-09-02 | Baker Brenda F. | Sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation |
US20050053976A1 (en) * | 1996-06-06 | 2005-03-10 | Baker Brenda F. | Chimeric oligomeric compounds and their use in gene modulation |
US7812149B2 (en) | 1996-06-06 | 2010-10-12 | Isis Pharmaceuticals, Inc. | 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations |
US20040161777A1 (en) * | 1996-06-06 | 2004-08-19 | Baker Brenda F. | Modified oligonucleotides for use in RNA interference |
US5807743A (en) * | 1996-12-03 | 1998-09-15 | Ribozyme Pharmaceuticals, Inc. | Interleukin-2 receptor gamma-chain ribozymes |
US6251666B1 (en) | 1997-03-31 | 2001-06-26 | Ribozyme Pharmaceuticals, Inc. | Nucleic acid catalysts comprising L-nucleotide analogs |
US6280936B1 (en) | 1997-06-09 | 2001-08-28 | Ribozyme Pharmaceuticals, Inc. | Method for screening nucleic acid catalysts |
US6548657B1 (en) | 1997-06-09 | 2003-04-15 | Ribozyme Pharmaceuticals, Inc. | Method for screening nucleic acid catalysts |
US6656731B1 (en) | 1997-09-22 | 2003-12-02 | Max Planck Gesellschaft Zur Forderung Der Wissenschaften E.V. | Nucleic acid catalysts with endonuclease activity |
AU750947C (en) * | 1997-09-22 | 2003-05-22 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Nucleic acid catalysts with endonuclease activity |
US6656692B2 (en) | 1999-12-21 | 2003-12-02 | Ingeneus Corporation | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
US6420115B1 (en) | 1999-12-21 | 2002-07-16 | Ingeneus Corporation | Cation mediated triplex hybridization assay |
US6858390B2 (en) | 1998-12-31 | 2005-02-22 | Ingeneus Corporation | Aptamers containing sequences of nucleic acid or nucleic acid analogues bound homologously, or in novel complexes |
US6403313B1 (en) | 1999-12-21 | 2002-06-11 | Ingeneus Corporation | Fluorescent intensity assay for duplex and triplex nucleic acid hybridization solution utilizing fluorescent intercalators |
US20030181412A1 (en) * | 1999-12-21 | 2003-09-25 | Ingeneus Corporation | Method for modifying transcription and/or translation in an organism for therapeutic, prophylactic and/or analytic uses |
US6924108B2 (en) | 1999-12-21 | 2005-08-02 | Ingeneus Corporation | Nucleic acid binding enhancement by conjugation with nucleotides, nucleosides, bases and/or their analogues |
US7309569B2 (en) | 1999-12-21 | 2007-12-18 | Ingeneus, Inc. | Parallel or antiparallel, homologous or complementary binding of nucleic acids or analogues thereof to form duplex, triplex or quadruplex complexes |
US7052844B2 (en) * | 1999-12-21 | 2006-05-30 | Ingeneus, Inc. | Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism |
US6911536B1 (en) | 1999-12-21 | 2005-06-28 | Ingeneus Corporation | Triplex and quadruplex catalytic hybridization |
US6927027B2 (en) | 1999-12-21 | 2005-08-09 | Ingeneus Corporation | Nucleic acid multiplex formation |
US6613524B1 (en) | 2000-01-24 | 2003-09-02 | Ingeneus Corporation | Amperometric affinity assay and electrically stimulated complexes of nucleic acids |
US7220541B2 (en) | 2000-01-24 | 2007-05-22 | Ingeneus, Inc. | Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions |
US20030170659A1 (en) * | 2000-01-24 | 2003-09-11 | Ingeneus Corporation | Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding |
US6982147B2 (en) * | 2000-01-24 | 2006-01-03 | Ingeneus Corporation | Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions |
US6831171B2 (en) | 2000-02-08 | 2004-12-14 | Yale University | Nucleic acid catalysts with endonuclease activity |
JP2003525037A (en) * | 2000-02-11 | 2003-08-26 | リボザイム・ファーマシューティカルズ・インコーポレーテッド | Methods and reagents for regulation and diagnosis of CD20 and NOGO gene expression |
US8202979B2 (en) * | 2002-02-20 | 2012-06-19 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid |
US7491805B2 (en) | 2001-05-18 | 2009-02-17 | Sirna Therapeutics, Inc. | Conjugates and compositions for cellular delivery |
US7833992B2 (en) | 2001-05-18 | 2010-11-16 | Merck Sharpe & Dohme | Conjugates and compositions for cellular delivery |
US20050032733A1 (en) * | 2001-05-18 | 2005-02-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SiNA) |
US20050233329A1 (en) * | 2002-02-20 | 2005-10-20 | Sirna Therapeutics, Inc. | Inhibition of gene expression using duplex forming oligonucleotides |
US8273866B2 (en) | 2002-02-20 | 2012-09-25 | Merck Sharp & Dohme Corp. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (SINA) |
US20080039414A1 (en) * | 2002-02-20 | 2008-02-14 | Sima Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
US7102024B1 (en) * | 2000-08-01 | 2006-09-05 | Schwartz David A | Functional biopolymer modification reagents and uses thereof |
US8568766B2 (en) | 2000-08-24 | 2013-10-29 | Gattadahalli M. Anantharamaiah | Peptides and peptide mimetics to treat pathologies associated with eye disease |
US20050209179A1 (en) * | 2000-08-30 | 2005-09-22 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA) |
US20080032942A1 (en) * | 2000-08-30 | 2008-02-07 | Mcswiggen James | RNA interference mediated treatment of Alzheimer's disease using short interfering nucleic acid (siNA) |
US20020150936A1 (en) * | 2000-09-01 | 2002-10-17 | Leonid Beigelman | Methods for synthesizing nucleosides, nucleoside derivatives and non-nucleoside derivatives |
AU8695901A (en) | 2000-09-01 | 2002-03-13 | Ribozyme Pharm Inc | Methods for synthesizing nucleosides, nucleoside derivatives and non-nucleoside derivatives |
EP1354062A2 (en) * | 2000-09-13 | 2003-10-22 | Archemix Corporation | Target activated nucleic acid biosensor and methods of using same |
US7125660B2 (en) | 2000-09-13 | 2006-10-24 | Archemix Corp. | Nucleic acid sensor molecules and methods of using same |
US20020165192A1 (en) * | 2000-09-19 | 2002-11-07 | Kerr William G. | Control of NK cell function and survival by modulation of ship activity |
US20110052546A1 (en) * | 2000-09-19 | 2011-03-03 | University Of South Florida | Inhibition of SHIP to Enhance Stem Cell Harvest and Transplantation |
EP2336166A1 (en) | 2000-10-12 | 2011-06-22 | University Of Rochester | Compositions that inhibit proliferation of cancer cells |
EP1386004A4 (en) | 2001-04-05 | 2005-02-16 | Ribozyme Pharm Inc | Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies |
US7517864B2 (en) * | 2001-05-18 | 2009-04-14 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20050148530A1 (en) | 2002-02-20 | 2005-07-07 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20050187174A1 (en) * | 2001-05-18 | 2005-08-25 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of intercellular adhesion molecule (ICAM) gene expression using short interfering nucleic acid (siNA) |
US20050079610A1 (en) * | 2001-05-18 | 2005-04-14 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of Fos gene expression using short interfering nucleic acid (siNA) |
US20060142226A1 (en) * | 2001-05-18 | 2006-06-29 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of cholesteryl ester transfer protein (CETP) gene expression using short interfering nucleic acid (siNA) |
US20040006035A1 (en) * | 2001-05-29 | 2004-01-08 | Dennis Macejak | Nucleic acid mediated disruption of HIV fusogenic peptide interactions |
US20040209831A1 (en) * | 2002-02-20 | 2004-10-21 | Mcswiggen James | RNA interference mediated inhibition of hepatitis C virus (HCV) gene expression using short interfering nucleic acid (siNA) |
US20050159376A1 (en) * | 2002-02-20 | 2005-07-21 | Slrna Therapeutics, Inc. | RNA interference mediated inhibition 5-alpha reductase and androgen receptor gene expression using short interfering nucleic acid (siNA) |
US20050222066A1 (en) * | 2001-05-18 | 2005-10-06 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20050288242A1 (en) * | 2001-05-18 | 2005-12-29 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of RAS gene expression using short interfering nucleic acid (siNA) |
US20050153915A1 (en) * | 2001-05-18 | 2005-07-14 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of early growth response gene expression using short interfering nucleic acid (siNA) |
US20050124566A1 (en) * | 2001-05-18 | 2005-06-09 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA) |
US20050176666A1 (en) * | 2001-05-18 | 2005-08-11 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of GPRA and AAA1 gene expression using short interfering nucleic acid (siNA) |
US20050203040A1 (en) * | 2001-05-18 | 2005-09-15 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of vascular cell adhesion molecule (VCAM) gene expression using short interfering nucleic acid (siNA) |
US20050287128A1 (en) * | 2001-05-18 | 2005-12-29 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA) |
US20080161256A1 (en) * | 2001-05-18 | 2008-07-03 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA) |
US20050124568A1 (en) * | 2001-05-18 | 2005-06-09 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of acetyl-CoA-carboxylase gene expression using short interfering nucleic acid (siNA) |
US20050119211A1 (en) * | 2001-05-18 | 2005-06-02 | Sirna Therapeutics, Inc. | RNA mediated inhibition connexin gene expression using short interfering nucleic acid (siNA) |
EP2415486B1 (en) | 2001-05-18 | 2017-02-22 | Sirna Therapeutics, Inc. | Conjugates and compositions for cellular delivery |
US20050227935A1 (en) * | 2001-05-18 | 2005-10-13 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of TNF and TNF receptor gene expression using short interfering nucleic acid (siNA) |
US20050196767A1 (en) * | 2001-05-18 | 2005-09-08 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of GRB2 associated binding protein (GAB2) gene expression using short interfering nucleic acis (siNA) |
US20050164968A1 (en) * | 2001-05-18 | 2005-07-28 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of ADAM33 gene expression using short interfering nucleic acid (siNA) |
US20050124567A1 (en) * | 2001-05-18 | 2005-06-09 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of TRPM7 gene expression using short interfering nucleic acid (siNA) |
US20050080031A1 (en) * | 2001-05-18 | 2005-04-14 | Sirna Therapeutics, Inc. | Nucleic acid treatment of diseases or conditions related to levels of Ras, HER2 and HIV |
US20050014172A1 (en) * | 2002-02-20 | 2005-01-20 | Ivan Richards | RNA interference mediated inhibition of muscarinic cholinergic receptor gene expression using short interfering nucleic acid (siNA) |
US20070042983A1 (en) * | 2001-05-18 | 2007-02-22 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA) |
US20040219671A1 (en) * | 2002-02-20 | 2004-11-04 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of parkinson disease using short interfering nucleic acid (siNA) |
US20050164966A1 (en) * | 2001-05-18 | 2005-07-28 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of type 1 insulin-like growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20050054596A1 (en) * | 2001-11-30 | 2005-03-10 | Mcswiggen James | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20050136436A1 (en) * | 2001-05-18 | 2005-06-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of G72 and D-amino acid oxidase (DAAO) gene expression using short interfering nucleic acid (siNA) |
US20050267058A1 (en) * | 2001-05-18 | 2005-12-01 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (sINA) |
US20080188430A1 (en) * | 2001-05-18 | 2008-08-07 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of hypoxia inducible factor 1 (HIF1) gene expression using short interfering nucleic acid (siNA) |
US20050282188A1 (en) * | 2001-05-18 | 2005-12-22 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA) |
US20060142225A1 (en) * | 2001-05-18 | 2006-06-29 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of cyclin dependent kinase-2 (CDK2) gene expression using short interfering nucleic acid (siNA) |
US20060241075A1 (en) * | 2001-05-18 | 2006-10-26 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of desmoglein gene expression using short interfering nucleic acid (siNA) |
US20050159380A1 (en) * | 2001-05-18 | 2005-07-21 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of angiopoietin gene expression using short interfering nucleic acid (siNA) |
US7109165B2 (en) | 2001-05-18 | 2006-09-19 | Sirna Therapeutics, Inc. | Conjugates and compositions for cellular delivery |
US20050153914A1 (en) * | 2001-05-18 | 2005-07-14 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of MDR P-glycoprotein gene expression using short interfering nucleic acid (siNA) |
US20050233997A1 (en) * | 2001-05-18 | 2005-10-20 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of matrix metalloproteinase 13 (MMP13) gene expression using short interfering nucleic acid (siNA) |
US20050164967A1 (en) * | 2001-05-18 | 2005-07-28 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of platelet-derived endothelial cell growth factor (ECGF1) gene expression using short interfering nucleic acid (siNA) |
US20050143333A1 (en) * | 2001-05-18 | 2005-06-30 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA) |
US20050233996A1 (en) * | 2002-02-20 | 2005-10-20 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of hairless (HR) gene expression using short interfering nucleic acid (siNA) |
US20050164224A1 (en) * | 2001-05-18 | 2005-07-28 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of cyclin D1 gene expression using short interfering nucleic acid (siNA) |
US20050176664A1 (en) * | 2001-05-18 | 2005-08-11 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of cholinergic muscarinic receptor (CHRM3) gene expression using short interfering nucleic acid (siNA) |
US20070270579A1 (en) * | 2001-05-18 | 2007-11-22 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using short interfering nucleic acid (siNA) |
US20050182009A1 (en) * | 2001-05-18 | 2005-08-18 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of NF-Kappa B / REL-A gene expression using short interfering nucleic acid (siNA) |
US20060211642A1 (en) * | 2001-05-18 | 2006-09-21 | Sirna Therapeutics, Inc. | RNA inteference mediated inhibition of hepatitis C virus (HVC) gene expression using short interfering nucleic acid (siNA) |
US9994853B2 (en) | 2001-05-18 | 2018-06-12 | Sirna Therapeutics, Inc. | Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference |
US20050256068A1 (en) * | 2001-05-18 | 2005-11-17 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of stearoyl-CoA desaturase (SCD) gene expression using short interfering nucleic acid (siNA) |
US20050196765A1 (en) * | 2001-05-18 | 2005-09-08 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of checkpoint Kinase-1 (CHK-1) gene expression using short interfering nucleic acid (siNA) |
US20050130181A1 (en) * | 2001-05-18 | 2005-06-16 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of wingless gene expression using short interfering nucleic acid (siNA) |
US20050182006A1 (en) * | 2001-05-18 | 2005-08-18 | Sirna Therapeutics, Inc | RNA interference mediated inhibition of protein kinase C alpha (PKC-alpha) gene expression using short interfering nucleic acid (siNA) |
US20050119212A1 (en) * | 2001-05-18 | 2005-06-02 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of FAS and FASL gene expression using short interfering nucleic acid (siNA) |
US20050191638A1 (en) * | 2002-02-20 | 2005-09-01 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of polyglutamine (polyQ) repeat expansion diseases using short interfering nucleic acid (siNA) |
US20050209180A1 (en) * | 2001-05-18 | 2005-09-22 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of hepatitis C virus (HCV) expression using short interfering nucleic acid (siNA) |
US20050137155A1 (en) * | 2001-05-18 | 2005-06-23 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of Parkinson disease using short interfering nucleic acid (siNA) |
US20050159379A1 (en) * | 2001-05-18 | 2005-07-21 | Sirna Therapeutics, Inc | RNA interference mediated inhibition of gastric inhibitory polypeptide (GIP) and gastric inhibitory polypeptide receptor (GIPR) gene expression using short interfering nucleic acid (siNA) |
US20050176665A1 (en) * | 2001-05-18 | 2005-08-11 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of hairless (HR) gene expression using short interfering nucleic acid (siNA) |
US20050182007A1 (en) * | 2001-05-18 | 2005-08-18 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of interleukin and interleukin receptor gene expression using short interfering nucleic acid (SINA) |
US20050159382A1 (en) * | 2001-05-18 | 2005-07-21 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of polycomb group protein EZH2 gene expression using short interfering nucleic acid (siNA) |
US20060019913A1 (en) * | 2001-05-18 | 2006-01-26 | Sirna Therapeutics, Inc. | RNA interference mediated inhibtion of protein tyrosine phosphatase-1B (PTP-1B) gene expression using short interfering nucleic acid (siNA) |
US20040198682A1 (en) * | 2001-11-30 | 2004-10-07 | Mcswiggen James | RNA interference mediated inhibition of placental growth factor gene expression using short interfering nucleic acid (siNA) |
US20050233344A1 (en) * | 2001-05-18 | 2005-10-20 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of platelet derived growth factor (PDGF) and platelet derived growth factor receptor (PDGFR) gene expression using short interfering nucleic acid (siNA) |
US20050054598A1 (en) * | 2002-02-20 | 2005-03-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition hairless (HR) gene expression using short interfering nucleic acid (siNA) |
US7785610B2 (en) * | 2001-06-21 | 2010-08-31 | Dynavax Technologies Corporation | Chimeric immunomodulatory compounds and methods of using the same—III |
EP2423335B1 (en) * | 2001-06-21 | 2014-05-14 | Dynavax Technologies Corporation | Chimeric immunomodulatory compounds and methods of using the same |
US20040126867A1 (en) * | 2001-07-06 | 2004-07-01 | Crooke Stanley T. | Human RNase III and compositions and uses thereof |
DE10150121B4 (en) * | 2001-10-11 | 2005-12-01 | Bernhard-Nocht-Institut für Tropenmedizin | Real-time detection of DNA amplification products |
US20050075304A1 (en) * | 2001-11-30 | 2005-04-07 | Mcswiggen James | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20040138163A1 (en) * | 2002-05-29 | 2004-07-15 | Mcswiggen James | RNA interference mediated inhibition of vascular edothelial growth factor and vascular edothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
US20070203333A1 (en) * | 2001-11-30 | 2007-08-30 | Mcswiggen James | RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA) |
EP1572902B1 (en) * | 2002-02-01 | 2014-06-11 | Life Technologies Corporation | HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES |
US20060009409A1 (en) | 2002-02-01 | 2006-01-12 | Woolf Tod M | Double-stranded oligonucleotides |
ATE508188T1 (en) | 2002-02-01 | 2011-05-15 | Life Technologies Corp | OLIGONUCLEOTIDE COMPOSITIONS WITH IMPROVED EFFECTIVENESS |
JP2005532263A (en) | 2002-02-06 | 2005-10-27 | ヴァイコー テクノロジーズ, インコーポレイテッド | Anti-infarction molecule |
US20050096284A1 (en) * | 2002-02-20 | 2005-05-05 | Sirna Therapeutics, Inc. | RNA interference mediated treatment of polyglutamine (polyQ) repeat expansion diseases using short interfering nucleic acid (siNA) |
WO2003106476A1 (en) * | 2002-02-20 | 2003-12-24 | Sirna Therapeutics, Inc | Nucleic acid mediated inhibition of enterococcus infection and cytolysin toxin activity |
US9657294B2 (en) | 2002-02-20 | 2017-05-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
EP1432724A4 (en) | 2002-02-20 | 2006-02-01 | Sirna Therapeutics Inc | Rna interference mediated inhibition of map kinase genes |
US20050137153A1 (en) * | 2002-02-20 | 2005-06-23 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of alpha-1 antitrypsin (AAT) gene expression using short interfering nucleic acid (siNA) |
JP2005517450A (en) * | 2002-02-20 | 2005-06-16 | サーナ・セラピューティクス・インコーポレイテッド | RNA interference-mediated target discovery and target evaluation using short interfering nucleic acids (siNA) |
US20050222064A1 (en) * | 2002-02-20 | 2005-10-06 | Sirna Therapeutics, Inc. | Polycationic compositions for cellular delivery of polynucleotides |
US9181551B2 (en) | 2002-02-20 | 2015-11-10 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
EP1534729A2 (en) * | 2002-02-26 | 2005-06-01 | University of Utah Research Foundation | Variants of nedd4l associated with hypertension and viral budding |
US20040248094A1 (en) * | 2002-06-12 | 2004-12-09 | Ford Lance P. | Methods and compositions relating to labeled RNA molecules that reduce gene expression |
US20100075423A1 (en) * | 2002-06-12 | 2010-03-25 | Life Technologies Corporation | Methods and compositions relating to polypeptides with rnase iii domains that mediate rna interference |
AU2003243541A1 (en) * | 2002-06-12 | 2003-12-31 | Ambion, Inc. | Methods and compositions relating to labeled rna molecules that reduce gene expression |
WO2004003233A1 (en) * | 2002-06-28 | 2004-01-08 | Rosetta Inpharmatics Llc | Methods to assess quality of microarrays |
US6989442B2 (en) * | 2002-07-12 | 2006-01-24 | Sirna Therapeutics, Inc. | Deprotection and purification of oligonucleotides and their derivatives |
US7655790B2 (en) * | 2002-07-12 | 2010-02-02 | Sirna Therapeutics, Inc. | Deprotection and purification of oligonucleotides and their derivatives |
AU2003257181A1 (en) | 2002-08-05 | 2004-02-23 | University Of Rochester | Protein transducing domain/deaminase chimeric proteins, related compounds, and uses thereof |
US20040058886A1 (en) * | 2002-08-08 | 2004-03-25 | Dharmacon, Inc. | Short interfering RNAs having a hairpin structure containing a non-nucleotide loop |
US20040029275A1 (en) * | 2002-08-10 | 2004-02-12 | David Brown | Methods and compositions for reducing target gene expression using cocktails of siRNAs or constructs expressing siRNAs |
NZ538628A (en) | 2002-08-12 | 2008-06-30 | Dynavax Tech Corp | Immunomodulatory compositions, methods of making, and methods of use thereof |
US7923547B2 (en) | 2002-09-05 | 2011-04-12 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA) |
WO2004022003A2 (en) | 2002-09-06 | 2004-03-18 | University Of South Florida | Materials and methods for treatment of allergic diseases |
US20080214437A1 (en) * | 2002-09-06 | 2008-09-04 | Mohapatra Shyam S | Methods and compositions for reducing activity of the atrial natriuretic peptide receptor and for treatment of diseases |
CA2498339A1 (en) * | 2002-09-19 | 2004-04-01 | Merck & Co., Inc. | Estrogen receptor modulators |
US20040242518A1 (en) * | 2002-09-28 | 2004-12-02 | Massachusetts Institute Of Technology | Influenza therapeutic |
CA2504720C (en) | 2002-11-05 | 2013-12-24 | Isis Pharmaceuticals, Inc. | Chimeric oligomeric compounds and their use in gene modulation |
WO2004044139A2 (en) | 2002-11-05 | 2004-05-27 | Isis Parmaceuticals, Inc. | Modified oligonucleotides for use in rna interference |
US20040231231A1 (en) * | 2002-12-20 | 2004-11-25 | Cataldo Dominic A. | Use of colloidal clays for sustained release of active ingredients |
US7625872B2 (en) * | 2002-12-23 | 2009-12-01 | Dynavax Technologies Corporation | Branched immunomodulatory compounds and methods of using the same |
AU2004231740A1 (en) * | 2003-04-17 | 2004-11-04 | The Trustees Of Columbia University In The City Ofnew York | Desmoglein 4 is a novel gene involved in hair growth |
EP3222294A1 (en) | 2003-04-30 | 2017-09-27 | Sirna Therapeutics, Inc. | Conjugates and compositions for cellular delivery |
EP1636342A4 (en) * | 2003-06-20 | 2008-10-08 | Isis Pharmaceuticals Inc | Oligomeric compounds for use in gene modulation |
US7807646B1 (en) * | 2003-11-20 | 2010-10-05 | University Of South Florida | SHIP-deficiency to increase megakaryocyte progenitor production |
EP1727556A2 (en) * | 2004-02-17 | 2006-12-06 | University Of South Florida | Materials and methods for treatment of inflammatory and cell proliferation disorders |
DK1716234T3 (en) * | 2004-02-20 | 2014-01-20 | Mologen Ag | Substituted, non-coding nucleic acid molecule for therapeutic and prophylactic immune stimulation in humans and higher animals |
US8569474B2 (en) | 2004-03-09 | 2013-10-29 | Isis Pharmaceuticals, Inc. | Double stranded constructs comprising one or more short strands hybridized to a longer strand |
EP1737878A2 (en) | 2004-04-05 | 2007-01-03 | Alnylam Pharmaceuticals Inc. | Process and reagents for oligonucleotide synthesis and purification |
WO2005105157A2 (en) | 2004-04-23 | 2005-11-10 | The Trustees Of Columbia University In The City Ofnew York | INHIBITION OF HAIRLESS PROTEIN mRNA |
WO2006078278A2 (en) | 2004-04-27 | 2006-07-27 | Alnylam Pharmaceuticals, Inc. | Single-stranded and double-stranded oligonucleotides comprising a 2-arylpropyl moiety |
EP1750776A2 (en) * | 2004-04-30 | 2007-02-14 | Alnylam Pharmaceuticals Inc. | Oligonucleotides comprising a c5-modified pyrimidine |
US20060040882A1 (en) | 2004-05-04 | 2006-02-23 | Lishan Chen | Compostions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells |
US10508277B2 (en) | 2004-05-24 | 2019-12-17 | Sirna Therapeutics, Inc. | Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference |
AU2005252662B2 (en) * | 2004-06-03 | 2011-08-18 | Isis Pharmaceuticals, Inc. | Double strand compositions comprising differentially modified strands for use in gene modulation |
US8394947B2 (en) | 2004-06-03 | 2013-03-12 | Isis Pharmaceuticals, Inc. | Positionally modified siRNA constructs |
EP1789553B1 (en) | 2004-06-30 | 2014-03-26 | Alnylam Pharmaceuticals Inc. | Oligonucleotides comprising a non-phosphate backbone linkage |
WO2006093526A2 (en) | 2004-07-21 | 2006-09-08 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a modified or non-natural nucleobase |
AU2005330637B2 (en) | 2004-08-04 | 2012-09-20 | Alnylam Pharmaceuticals, Inc. | Oligonucleotides comprising a ligand tethered to a modified or non-natural nucleobase |
PL1794174T3 (en) * | 2004-09-01 | 2012-11-30 | Dynavax Tech Corp | Methods and compositions for inhibition of innate immune responses and autoimmunity |
US7884086B2 (en) | 2004-09-08 | 2011-02-08 | Isis Pharmaceuticals, Inc. | Conjugates for use in hepatocyte free uptake assays |
WO2006066003A2 (en) * | 2004-12-17 | 2006-06-22 | Dynavax Technologies Corporation | Methods and compositions for induction or promotion of immune tolerance |
US20060142228A1 (en) * | 2004-12-23 | 2006-06-29 | Ambion, Inc. | Methods and compositions concerning siRNA's as mediators of RNA interference |
AU2006210838B2 (en) | 2005-02-02 | 2011-10-20 | The Uab Research Foundation | Agents and methods related to reducing resistance to apoptosis-inducing death receptor agonists |
SG10201912554TA (en) | 2005-03-23 | 2020-02-27 | Genmab As | Antibodies against cd38 for treatment of multiple myeloma |
US7476733B2 (en) * | 2005-03-25 | 2009-01-13 | The United States Of America As Represented By The Department Of Health And Human Services | Development of a real-time PCR assay for detection of pneumococcal DNA and diagnosis of pneumococccal disease |
US20090203055A1 (en) * | 2005-04-18 | 2009-08-13 | Massachusetts Institute Of Technology | Compositions and methods for RNA interference with sialidase expression and uses thereof |
US8252756B2 (en) | 2005-06-14 | 2012-08-28 | Northwestern University | Nucleic acid functionalized nanoparticles for therapeutic applications |
WO2007015348A1 (en) | 2005-08-04 | 2007-02-08 | Sharp Kabushiki Kaisha | Display device and its drive method |
US20090176725A1 (en) * | 2005-08-17 | 2009-07-09 | Sirna Therapeutics Inc. | Chemically modified short interfering nucleic acid molecules that mediate rna interference |
EP1934244A2 (en) * | 2005-10-06 | 2008-06-25 | Emthrax LLC | Methods and compositions relating to anthrax spore glycoproteins as vaccines |
US8080534B2 (en) | 2005-10-14 | 2011-12-20 | Phigenix, Inc | Targeting PAX2 for the treatment of breast cancer |
EP2189522A1 (en) | 2005-10-14 | 2010-05-26 | MUSC Foundation For Research Development | Targeting PAX2 for the induction of DEFB1-mediated tumor immunity and cancer therapy |
WO2007127439A2 (en) | 2006-04-28 | 2007-11-08 | Children's Hospital Medical Center | Compositions comprising fusogenic proteins or polypeptides derived from prosaposin for application in transmembrane drug delivery systems |
WO2007127487A2 (en) * | 2006-04-28 | 2007-11-08 | University Of South Florida | Materials and methods for reducing inflammation by inhibition of the atrial natriuretic peptide receptor |
JP5926475B2 (en) | 2006-09-21 | 2016-05-25 | ユニバーシティー オブ ロチェスター | Compositions and methods for protein replacement therapy for myotonic dystrophy |
WO2008136852A2 (en) | 2006-11-01 | 2008-11-13 | University Of Rochester | Methods and compositions related to the structure and function of apobec3g |
JP2010512327A (en) | 2006-12-11 | 2010-04-22 | ユニヴァーシティー オブ ユタ リサーチ ファウンデーション | Compositions and methods for the treatment of pathological angiogenesis and vascular permeability |
US7951789B2 (en) * | 2006-12-28 | 2011-05-31 | Idenix Pharmaceuticals, Inc. | Compounds and pharmaceutical compositions for the treatment of viral infections |
MX2009008470A (en) | 2007-02-09 | 2009-11-26 | Univ Northwestern | Particles for detecting intracellular targets. |
JP2010519256A (en) | 2007-02-23 | 2010-06-03 | ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク | Methods of activating or blocking the HLA-E / Qa-1 restricted CD8 + T cell regulatory pathway to treat immune diseases |
WO2008104978A2 (en) * | 2007-02-28 | 2008-09-04 | Quark Pharmaceuticals, Inc. | Novel sirna structures |
HUE040417T2 (en) | 2007-05-04 | 2019-03-28 | Marina Biotech Inc | Amino acid lipids and uses thereof |
AU2008259907B2 (en) | 2007-05-30 | 2014-12-04 | Northwestern University | Nucleic acid functionalized nanoparticles for therapeutic applications |
WO2009032693A2 (en) | 2007-08-28 | 2009-03-12 | Uab Research Foundation | Synthetic apolipoprotein e mimicking polypeptides and methods of use |
JP2010537638A (en) | 2007-08-28 | 2010-12-09 | ユーエービー リサーチ ファウンデーション | Synthetic apolipoprotein E mimetic polypeptides and methods of use |
US20100136026A1 (en) * | 2007-09-26 | 2010-06-03 | Kerr William G | Ship Inhibition to Direct Hematopoietic Stem Cells and Induce Extramedullary Hematopoiesis |
JP5646997B2 (en) * | 2007-10-03 | 2014-12-24 | クォーク ファーマシューティカルズ インコーポレーティッドQuark Pharmaceuticals,Inc. | Novel siRNA structure |
EP2209896B1 (en) * | 2007-10-26 | 2017-03-01 | Dynavax Technologies Corporation | Methods and compositions for inhibition of immune responses and autoimmunity |
EP2268664B1 (en) | 2007-12-03 | 2017-05-24 | The Government of the United States of America as represented by the Secretary of the Department of Health and Human Services | Doc1 compositions and methods for treating cancer |
US20110105584A1 (en) * | 2007-12-12 | 2011-05-05 | Elena Feinstein | Rtp80il sirna compounds and methods of use thereof |
US8614311B2 (en) | 2007-12-12 | 2013-12-24 | Quark Pharmaceuticals, Inc. | RTP801L siRNA compounds and methods of use thereof |
WO2009090639A2 (en) * | 2008-01-15 | 2009-07-23 | Quark Pharmaceuticals, Inc. | Sirna compounds and methods of use thereof |
WO2009102427A2 (en) * | 2008-02-11 | 2009-08-20 | Rxi Pharmaceuticals Corp. | Modified rnai polynucleotides and uses thereof |
US20090233993A1 (en) * | 2008-03-06 | 2009-09-17 | Burnham Institute For Medical Research | Compositions and methods for inhibiting gsk3 activity and uses thereof |
EP2268316A4 (en) * | 2008-03-20 | 2011-05-25 | Quark Pharmaceuticals Inc | NOVEL siRNA COMPOUNDS FOR INHIBITING RTP801 |
WO2009144704A2 (en) * | 2008-04-15 | 2009-12-03 | Quark Pharmaceuticals, Inc. | siRNA COMPOUNDS FOR INHIBITING NRF2 |
WO2009137686A1 (en) | 2008-05-08 | 2009-11-12 | University Of Utah Research Foundation | Sensory receptors for chronic fatigue and pain and uses thereof |
US20110229498A1 (en) | 2008-05-08 | 2011-09-22 | The Johns Hopkins University | Compositions and methods for modulating an immune response |
ATE550024T1 (en) | 2008-05-30 | 2012-04-15 | Univ Yale | TARGETED OLIGONUCLEOTIDE COMPOSITIONS FOR MODIFYING GENE EXPRESSION |
AU2009277172B2 (en) * | 2008-07-02 | 2014-05-29 | Idenix Pharmaceuticals, Inc. | Compounds and pharmaceutical compositions for the treatment of viral infections |
WO2010008582A2 (en) | 2008-07-18 | 2010-01-21 | Rxi Pharmaceuticals Corporation | Phagocytic cell drug delivery system |
US10138485B2 (en) | 2008-09-22 | 2018-11-27 | Rxi Pharmaceuticals Corporation | Neutral nanotransporters |
CN104382853A (en) | 2008-10-16 | 2015-03-04 | 玛瑞纳生物技术有限公司 | Processes and Compositions for Liposomal and Efficient Delivery of Gene Silencing Therapeutics |
WO2010059226A2 (en) | 2008-11-19 | 2010-05-27 | Rxi Pharmaceuticals Corporation | Inhibition of map4k4 through rnai |
AU2009316286B2 (en) | 2008-11-24 | 2016-05-26 | Northwestern University | Polyvalent RNA-nanoparticle compositions |
WO2010065617A1 (en) | 2008-12-02 | 2010-06-10 | University Of Utah Research Foundation | Pde1 as a target therapeutic in heart disease |
WO2010078536A1 (en) | 2009-01-05 | 2010-07-08 | Rxi Pharmaceuticals Corporation | Inhibition of pcsk9 through rnai |
US20100233270A1 (en) | 2009-01-08 | 2010-09-16 | Northwestern University | Delivery of Oligonucleotide-Functionalized Nanoparticles |
US9745574B2 (en) | 2009-02-04 | 2017-08-29 | Rxi Pharmaceuticals Corporation | RNA duplexes with single stranded phosphorothioate nucleotide regions for additional functionality |
US20110038871A1 (en) | 2009-08-11 | 2011-02-17 | Veena Viswanth | Ccr2 inhibitors for treating conditions of the eye |
WO2011031974A1 (en) | 2009-09-10 | 2011-03-17 | Southern Research Institute | Acridine analogs in the treatment of gliomas |
CN102666879B (en) | 2009-10-30 | 2016-02-24 | 西北大学 | Templated nanometer conjugate |
CA2776568A1 (en) | 2009-11-26 | 2011-06-03 | Quark Pharmaceuticals, Inc. | Sirna compounds comprising terminal substitutions |
TWI465238B (en) | 2009-12-09 | 2014-12-21 | Nitto Denko Corp | Modulation of hsp47 expression |
WO2011084193A1 (en) | 2010-01-07 | 2011-07-14 | Quark Pharmaceuticals, Inc. | Oligonucleotide compounds comprising non-nucleotide overhangs |
US20110207789A1 (en) | 2010-02-19 | 2011-08-25 | Ye Fang | Methods related to casein kinase ii (ck2) inhibitors and the use of purinosome-disrupting ck2 inhibitors for anti-cancer therapy agents |
KR20180044433A (en) | 2010-03-24 | 2018-05-02 | 알엑스아이 파마슈티칼스 코포레이션 | Rna interference in dermal and fibrotic indications |
US9095504B2 (en) | 2010-03-24 | 2015-08-04 | Rxi Pharmaceuticals Corporation | RNA interference in ocular indications |
RU2615143C2 (en) | 2010-03-24 | 2017-04-04 | Адвирна | Self-delivered rnai compounds of reduced size |
WO2011120023A1 (en) | 2010-03-26 | 2011-09-29 | Marina Biotech, Inc. | Nucleic acid compounds for inhibiting survivin gene expression uses thereof |
MX2012011222A (en) | 2010-04-01 | 2013-01-18 | Centre Nat Rech Scient | Compounds and pharmaceutical compositions for the treatment of viral infections. |
DK2558578T3 (en) | 2010-04-13 | 2016-01-25 | Life Technologies Corp | CONFIGURATIONS AND METHODS FOR INHIBITION OF nucleic acid function |
WO2011133584A2 (en) | 2010-04-19 | 2011-10-27 | Marina Biotech, Inc. | Nucleic acid compounds for inhibiting hras gene expression and uses thereof |
WO2011139710A1 (en) | 2010-04-26 | 2011-11-10 | Marina Biotech, Inc. | Nucleic acid compounds with conformationally restricted monomers and uses thereof |
WO2011139842A2 (en) | 2010-04-28 | 2011-11-10 | Marina Biotech, Inc. | Nucleic acid compounds for inhibiting fgfr3 gene expression and uses thereof |
KR101810593B1 (en) | 2010-06-16 | 2017-12-22 | 다이나박스 테크놀로지 코퍼레이션 | Methods of treatment using tlr7 and/or tlr9 inhibitors |
EP3372684B1 (en) | 2010-08-24 | 2020-10-07 | Sirna Therapeutics, Inc. | Single-stranded rnai agents containing an internal, non-nucleic acid spacer |
WO2012058210A1 (en) | 2010-10-29 | 2012-05-03 | Merck Sharp & Dohme Corp. | RNA INTERFERENCE MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACIDS (siNA) |
EP2681314B1 (en) | 2011-03-03 | 2017-11-01 | Quark Pharmaceuticals, Inc. | Compositions and methods for treating lung disease and injury |
US10184942B2 (en) | 2011-03-17 | 2019-01-22 | University Of South Florida | Natriuretic peptide receptor as a biomarker for diagnosis and prognosis of cancer |
WO2012154321A1 (en) | 2011-03-31 | 2012-11-15 | Idenix Pharmaceuticals, Inc. | Compounds and pharmaceutical compositions for the treatment of viral infections |
US10196637B2 (en) | 2011-06-08 | 2019-02-05 | Nitto Denko Corporation | Retinoid-lipid drug carrier |
TWI658830B (en) | 2011-06-08 | 2019-05-11 | 日東電工股份有限公司 | Retinoid-liposomes for enhancing modulation of hsp47 expression |
US9751909B2 (en) | 2011-09-07 | 2017-09-05 | Marina Biotech, Inc. | Synthesis and uses of nucleic acid compounds with conformationally restricted monomers |
TW201329096A (en) | 2011-09-12 | 2013-07-16 | Idenix Pharmaceuticals Inc | Substituted carbonyloxymethylphosphoramidate compounds and pharmaceutical compositions for the treatment of viral infections |
AU2012308302A1 (en) | 2011-09-14 | 2014-03-20 | Northwestern University | Nanoconjugates able to cross the blood-brain barrier |
PT3597644T (en) | 2011-10-18 | 2021-11-03 | Dicerna Pharmaceuticals Inc | Amine cationic lipids and uses thereof |
EP2877490B1 (en) | 2012-06-27 | 2018-09-05 | The Trustees of Princeton University | Split inteins, conjugates and uses thereof |
EP2873732A4 (en) | 2012-07-16 | 2016-03-23 | Kyowa Hakko Kirin Co Ltd | Rnai pharmaceutical composition capable of suppressing expression of kras gene |
US9611473B2 (en) | 2012-09-12 | 2017-04-04 | Quark Pharmaceuticals, Inc. | Double-stranded nucleic acid compounds |
US9228184B2 (en) | 2012-09-29 | 2016-01-05 | Dynavax Technologies Corporation | Human toll-like receptor inhibitors and methods of use thereof |
KR102205278B1 (en) | 2013-03-14 | 2021-01-22 | 다이서나 파마수이티컬, 인크. | Process for formulating an anionic agent |
WO2015027176A1 (en) * | 2013-08-22 | 2015-02-26 | Sony Corporation | Water soluble fluorescent or colored dyes and methods for their use |
EP3079707A4 (en) | 2013-12-02 | 2017-10-18 | RXi Pharmaceuticals Corporation | Immunotherapy of cancer |
EP3087184B1 (en) | 2013-12-27 | 2019-07-03 | Dicerna Pharmaceuticals, Inc. | Methods and compositions for the specific inhibition of glycolate oxidase (hao1) by double-stranded rna |
EP3137119B1 (en) | 2014-04-28 | 2020-07-01 | Phio Pharmaceuticals Corp. | Methods for treating cancer using a nucleic acid targeting mdm2 |
CN106535876B (en) | 2014-06-04 | 2020-09-11 | 埃克西奎雷股份有限公司 | Multivalent delivery of immunomodulators through liposomal spherical nucleic acids for prophylactic or therapeutic applications |
AU2015298263B2 (en) | 2014-07-31 | 2020-05-14 | Anji Pharmaceuticals, Inc. | ApoE mimetic peptides and higher potency to clear plasma cholesterol |
WO2016022866A1 (en) | 2014-08-07 | 2016-02-11 | Agilent Technologies, Inc. | Cis-blocked guide rna |
CN107073294A (en) | 2014-09-05 | 2017-08-18 | 阿克赛医药公司 | Use the method for targeting TYR or MMP1 exonuclease treatment aging and skin disorder |
JP2017537619A (en) | 2014-11-21 | 2017-12-21 | ノースウェスタン ユニバーシティ | Sequence-specific intracellular uptake of spherical nucleic acid nanoparticle complexes |
US10264976B2 (en) | 2014-12-26 | 2019-04-23 | The University Of Akron | Biocompatible flavonoid compounds for organelle and cell imaging |
WO2017007825A1 (en) | 2015-07-06 | 2017-01-12 | Rxi Pharmaceuticals Corporation | Methods for treating neurological disorders using a synergistic small molecule and nucleic acids therapeutic approach |
JP6983752B2 (en) | 2015-07-06 | 2021-12-17 | フィオ ファーマシューティカルズ コーポレーションPhio Pharmaceuticals Corp. | Nucleic acid molecule targeting superoxide dismutase 1 (SOD1) |
LU92821B1 (en) | 2015-09-09 | 2017-03-20 | Mologen Ag | Combination comprising immunostimulatory oligonucleotides |
GB2542425A (en) | 2015-09-21 | 2017-03-22 | Mologen Ag | Means for the treatment of HIV |
CA3002744A1 (en) | 2015-10-19 | 2017-04-27 | Rxi Pharmaceuticals Corporation | Reduced size self-delivering nucleic acid compounds targeting long non-coding rna |
CA3005937C (en) | 2015-12-13 | 2021-11-09 | Nitto Denko Corporation | Sirna structures for high activity and reduced off target |
WO2017131236A1 (en) | 2016-01-29 | 2017-08-03 | 協和発酵キリン株式会社 | Nucleic acid complex |
JP7022687B2 (en) | 2016-06-30 | 2022-02-18 | 協和キリン株式会社 | Nucleic acid complex |
CN110087665A (en) | 2016-08-03 | 2019-08-02 | H·李·莫菲特癌症中心与研究所公司 | TLR9 targeted therapy |
US11364304B2 (en) | 2016-08-25 | 2022-06-21 | Northwestern University | Crosslinked micellar spherical nucleic acids |
AU2019390097A1 (en) | 2018-11-30 | 2021-07-15 | Kyowa Kirin Co., Ltd. | Nucleic acid conjugate |
JP7398007B2 (en) | 2020-03-18 | 2023-12-13 | ディセルナ ファーマシューティカルズ インコーポレイテッド | Compositions and methods for inhibiting ANGPTL3 expression |
WO2022031847A2 (en) | 2020-08-04 | 2022-02-10 | Dicerna Pharmaceuticals Inc. | Compositions and methods for inhibiting plp1 expression |
TW202221120A (en) | 2020-08-04 | 2022-06-01 | 美商黛瑟納製藥公司 | Compositions and methods for the treatment of metabolic syndrome |
IL300338A (en) | 2020-08-05 | 2023-04-01 | Dicerna Pharmaceuticals Inc | Compositions and methods of inhibiting lpa expression |
US20220340909A1 (en) | 2021-04-12 | 2022-10-27 | Boehringer Ingelheim International Gmbh | Compositions and methods for inhibiting ketohexokinase (khk) |
WO2022221430A1 (en) | 2021-04-14 | 2022-10-20 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for modulating pnpla3 expression |
US11578329B2 (en) | 2021-04-19 | 2023-02-14 | Novo Nordisk A/S | Compositions and methods for inhibiting nuclear receptor subfamily 1 group H member 3 (NR1H3) expression |
JP7463621B2 (en) | 2021-05-28 | 2024-04-08 | ノヴォ ノルディスク アー/エス | Compositions and methods for inhibiting mitochondrial amidoxime reducing component 1 (MARC1) expression |
TW202308660A (en) | 2021-08-25 | 2023-03-01 | 美商戴瑟納製藥股份有限公司 | Compositions and methods for inhibiting alpha-1 antitrypsin expression |
US20230159930A1 (en) | 2021-11-19 | 2023-05-25 | Sanegene Bio Usa Inc. | Double stranded rna targeting angiopoietin-like 3 (angptl-3) and methods of use thereof |
US20230272393A1 (en) | 2021-12-01 | 2023-08-31 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for modulating apoc3 expression |
US20230374522A1 (en) | 2022-04-15 | 2023-11-23 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for modulating scap activity |
US20240002857A1 (en) | 2022-05-09 | 2024-01-04 | Sanegene Bio Usa Inc. | Double stranded rna targeting 17-beta hydroxysteroiddehydrogenase 13 (hsd17b13) and methods of use thereof |
WO2023220349A1 (en) | 2022-05-12 | 2023-11-16 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for inhibiting mapt expression |
US20230416743A1 (en) | 2022-05-13 | 2023-12-28 | Dicerna Pharmaceuticals, Inc. | Compositions and methods for inhibiting snca expression |
TW202400193A (en) | 2022-06-24 | 2024-01-01 | 丹麥商諾佛 儂迪克股份有限公司 | Compositions and methods for inhibiting transmembrane serine protease 6 (tmprss6) expression |
WO2024031101A1 (en) | 2022-08-05 | 2024-02-08 | Sanegene Bio Usa Inc. | Double stranded rna targeting angiotensinogen (agt) and methods of use thereof |
WO2024040041A1 (en) | 2022-08-15 | 2024-02-22 | Dicerna Pharmaceuticals, Inc. | Regulation of activity of rnai molecules |
WO2024079071A1 (en) | 2022-10-11 | 2024-04-18 | Boehringer Ingelheim International Gmbh | Dosage regimen for the treatment of nash |
WO2024079076A1 (en) | 2022-10-11 | 2024-04-18 | Boehringer Ingelheim International Gmbh | Methods for the treatment of nash with advanced fibrosis and/or cirrhosis |
WO2024081954A2 (en) | 2022-10-14 | 2024-04-18 | Sanegene Bio Usa Inc. | Small interfering rna targeting c3 and uses thereof |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4987071A (en) * | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
PT88550A (en) | 1987-09-21 | 1989-07-31 | Ml Tecnology Ventures Lp | PROCESS FOR THE PREPARATION OF NON-NUCLEOTIDIC LIGACATION REAGENTS FOR NUCLEOTIDIAL PROBES |
CA1340323C (en) * | 1988-09-20 | 1999-01-19 | Arnold E. Hampel | Rna catalyst for cleaving specific rna sequences |
US5262530A (en) * | 1988-12-21 | 1993-11-16 | Applied Biosystems, Inc. | Automated system for polynucleotide synthesis and purification |
JP3058686B2 (en) | 1989-08-31 | 2000-07-04 | シティ・オブ・ホープ | Chimeric DNA-RNA catalytically active sequence |
JPH05509224A (en) * | 1990-06-19 | 1993-12-22 | コモンウェルス サイエンティフィク アンド インダストリアル リサーチ オーガナイゼイション | endonuclease |
US6365730B1 (en) * | 1990-06-19 | 2002-04-02 | Gene Shears Pty. Limited | DNA-Armed ribozymes and minizymes |
DE552178T1 (en) | 1990-10-12 | 1994-02-03 | Max Planck Gesellschaft | MODIFIED RIBOZYMS. |
DE4216134A1 (en) * | 1991-06-20 | 1992-12-24 | Europ Lab Molekularbiolog | SYNTHETIC CATALYTIC OLIGONUCLEOTIDE STRUCTURES |
US5652094A (en) * | 1992-01-31 | 1997-07-29 | University Of Montreal | Nucleozymes |
WO1993023569A1 (en) * | 1992-05-11 | 1993-11-25 | Ribozyme Pharmaceuticals, Inc. | Method and reagent for inhibiting viral replication |
EP0786522A2 (en) * | 1992-07-17 | 1997-07-30 | Ribozyme Pharmaceuticals, Inc. | Enzymatic RNA molecules for treatment of stenotic conditions |
GB9225427D0 (en) * | 1992-12-04 | 1993-01-27 | Ribonetics Gmbh | Oligonucleotides with rna cleavage activity |
JPH09502092A (en) | 1993-09-02 | 1997-03-04 | リボザイム・ファーマシューティカルズ・インコーポレイテッド | Enzymatic nucleic acid containing non-nucleotide |
US5672501A (en) * | 1994-12-23 | 1997-09-30 | Ribozyme Pharmaceuticals, Inc. | Base-modified enzymatic nucleic acid |
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1994
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- 1994-08-19 ES ES94925939T patent/ES2186690T3/en not_active Expired - Lifetime
- 1994-08-19 CA CA002169645A patent/CA2169645A1/en not_active Abandoned
- 1994-08-19 DK DK94925939T patent/DK0748382T3/en active
- 1994-08-19 PT PT94925939T patent/PT748382E/en unknown
- 1994-08-19 EP EP05015038A patent/EP1602725A3/en not_active Withdrawn
- 1994-08-19 AT AT94925939T patent/ATE227342T1/en not_active IP Right Cessation
- 1994-08-19 WO PCT/US1994/009342 patent/WO1995006731A2/en active IP Right Grant
- 1994-08-19 EP EP02009782A patent/EP1253199A1/en not_active Withdrawn
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US6117657A (en) | 2000-09-12 |
US6362323B1 (en) | 2002-03-26 |
PT748382E (en) | 2003-03-31 |
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