CA2229568A1 - Quiescent cell populations for nuclear transfer - Google Patents
Quiescent cell populations for nuclear transferInfo
- Publication number
- CA2229568A1 CA2229568A1 CA002229568A CA2229568A CA2229568A1 CA 2229568 A1 CA2229568 A1 CA 2229568A1 CA 002229568 A CA002229568 A CA 002229568A CA 2229568 A CA2229568 A CA 2229568A CA 2229568 A1 CA2229568 A1 CA 2229568A1
- Authority
- CA
- Canada
- Prior art keywords
- animal
- embryo
- cell
- donor cell
- reconstituted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/027—New breeds of vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8773—Ovine embryos
Abstract
A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals.
The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
Claims (19)
1. A method of reconstituting an animal embryo, the method comprising transferring the nucleus of a quiescent donor cell into a suitable recipient cell.
2. A method as claimed in claim 1, in which the animal is an ungulate species.
3. A method as claimed in claim 2, in which the animal is a cow or bull, pig, goat, sheep, camel or water buffalo.
4. A method as claimed in any one of claims 1 to 3, in which the nucleus of the donor cell is genetically modified.
5. A method as claimed in claim 4, in which the donor cell is genetically modified prior to embryo reconstitution.
6. A method as claimed in claim 5, in which the recipient cell is an oocyte.
7. A method as claimed in claim 6, in which the oocyte is enucleate.
8. A method as claimed in any one of claims 1 to 7, in which the donor cell is an adult somatic cell.
9. A method as claimed in any one of claims 1 to 7, in which the donor cell is an embryonic somatic cell.
10. A method as claimed in any one of claims 1 to 7, in which the donor cell is a foetal somatic cell.
11. A method for preparing an animal, the method comprising:
(a) reconstituting an animal embryo as claimed in any preceding claim;
(b) causing an animal to develop to term from the embryo; and (c) optionally, breeding from the animal so formed.
(a) reconstituting an animal embryo as claimed in any preceding claim;
(b) causing an animal to develop to term from the embryo; and (c) optionally, breeding from the animal so formed.
12. A method as claimed in claim 11, in which the animal embryo is further manipulated prior to full development of the embryo.
13. A method as claimed in claim 12, wherein more than one animal is derived from the embryo.
14. A reconstituted animal embryo prepared by transferring the nucleus of a quiescent donor cell into a suitable recipient cell.
15. A reconstituted embryo as claimed in claim 14, in which the donor cell is an adult somatic cell.
16. A reconstituted embryo as claimed in claim 14, in which the donor cell is an embryonic somatic cell.
17. A reconstituted embryo as claimed in claim 14, in which the donor cell is a foetal somatic cell.
18. An animal prepared by a method as claimed in any one of claims 1 to 13.
19. An animal developed from a reconstituted animal embryo as claimed in any one of claims 14 to 17.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB9517780.4 | 1995-08-31 | ||
GBGB9517780.4A GB9517780D0 (en) | 1995-08-31 | 1995-08-31 | Biological manipulation |
PCT/GB1996/002099 WO1997007669A1 (en) | 1995-08-31 | 1996-08-30 | Quiescent cell populations for nuclear transfer |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2229568A1 true CA2229568A1 (en) | 1997-03-06 |
CA2229568C CA2229568C (en) | 2011-11-22 |
Family
ID=10779998
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2229568A Expired - Lifetime CA2229568C (en) | 1995-08-31 | 1996-08-30 | Quiescent cell populations for nuclear transfer |
Country Status (24)
Country | Link |
---|---|
US (5) | US6147276A (en) |
EP (3) | EP0849990B1 (en) |
JP (2) | JP4081613B2 (en) |
KR (4) | KR20060053008A (en) |
CN (3) | CN100422317C (en) |
AT (1) | ATE199115T1 (en) |
AU (1) | AU716956C (en) |
BR (1) | BR9610034B1 (en) |
CA (1) | CA2229568C (en) |
CZ (1) | CZ60898A3 (en) |
DE (1) | DE69611793T2 (en) |
DK (1) | DK0849990T3 (en) |
ES (1) | ES2155197T3 (en) |
GB (2) | GB9517780D0 (en) |
HK (2) | HK1004938A1 (en) |
HU (1) | HUP9900234A3 (en) |
IL (1) | IL123298A0 (en) |
NO (1) | NO980845L (en) |
NZ (3) | NZ337311A (en) |
PL (1) | PL325331A1 (en) |
SG (1) | SG75155A1 (en) |
SI (1) | SI0849990T1 (en) |
WO (1) | WO1997007669A1 (en) |
ZA (1) | ZA967390B (en) |
Families Citing this family (224)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5480772A (en) | 1993-02-03 | 1996-01-02 | Brandeis University | In vitro activation of a nucleus |
US7307198B2 (en) | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
GB9517779D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
GB9517780D0 (en) * | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
US6025155A (en) * | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US6077697A (en) * | 1996-04-10 | 2000-06-20 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US7696404B2 (en) | 1996-08-19 | 2010-04-13 | Advanced Cell Technology, Inc. | Embryonic or stem-like cell lines produced by cross species nuclear transplantation and methods for enhancing embryonic development by genetic alteration of donor cells or by tissue culture conditions |
WO1998016630A1 (en) | 1996-10-11 | 1998-04-23 | The Texas A & M University System | Methods for the generation of primordial germ cells and transgenic animal species |
US6235969B1 (en) * | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
US5945577A (en) * | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
US20090092588A1 (en) * | 1997-01-10 | 2009-04-09 | University Of Massachusetts As Represented By Its Amherst Campus | Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease |
US6215041B1 (en) * | 1997-01-10 | 2001-04-10 | University Of Mmassachusetts | Cloning using donor nuclei from a non-quiesecent somatic cells |
GB9703550D0 (en) * | 1997-02-20 | 1997-04-09 | Ppl Therapeutics Scotland Ltd | Cell mediated transgenesis |
US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
AU744445B2 (en) * | 1997-04-24 | 2002-02-21 | University Of Washington | Targeted gene modification by parvoviral vectors |
US6921814B2 (en) | 1997-06-19 | 2005-07-26 | The General Hospital Corporation | Torsin, torsin-related genes and methods of detecting neuronal disease |
JP4323572B2 (en) | 1997-06-19 | 2009-09-02 | ザ ジェネラル ホスピタル コーポレーション | Torsin, torsin gene, and usage |
EP1017422A4 (en) * | 1997-07-03 | 2002-10-29 | Univ Massachusetts | Cloning using donor nuclei from non-serum starved, differentiated cells |
WO1999005266A2 (en) * | 1997-07-26 | 1999-02-04 | Wisconsin Alumni Research Foundation | Trans-species nuclear transfer |
JP2001513336A (en) * | 1997-08-22 | 2001-09-04 | ロスリン インスティチュート(エディンバラ) | Use of "marina" transposans in the production of transgenic animals |
AU1581899A (en) * | 1997-10-28 | 1999-05-17 | Biotransplant Incorporated | Nuclear transfer for production of transgenic animal embryo |
EP0972017B1 (en) | 1998-01-16 | 2004-10-06 | Agrobiogen GmbH | Efficient nuclear transfer using fetal fibroblasts |
US6331659B1 (en) * | 1998-01-21 | 2001-12-18 | University Of Hawaii | Cumulus cells as nuclear donors |
EP1067834A4 (en) * | 1998-03-16 | 2002-01-23 | Garelag Pty Ltd | Porcine nuclear transfer |
AUPP294898A0 (en) * | 1998-04-15 | 1998-05-07 | Monash University | Method of nuclear transfer |
GB9808325D0 (en) * | 1998-04-20 | 1998-06-17 | Ltr Ciz Di Associazione Italia | Source of nuclei for nuclear transfer |
GB9809178D0 (en) * | 1998-04-29 | 1998-07-01 | Univ Edinburgh | Nuclear reprogramming of somatic cells |
WO2000022098A1 (en) | 1998-10-12 | 2000-04-20 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
EP1375654A3 (en) * | 1998-11-02 | 2008-01-16 | GTC Biotherapeutics, Inc. | Transgenic and cloned mammals |
US6580017B1 (en) | 1998-11-02 | 2003-06-17 | Genzyme Transgenics Corporation | Methods of reconstructed goat embryo transfer |
JP4523165B2 (en) * | 1998-11-02 | 2010-08-11 | トラスティーズ オブ タフツ カレッジ | Transgenic and cloned mammals |
US6781030B1 (en) | 1998-11-02 | 2004-08-24 | Trustee Of Tufts College, Ballou Hall | Methods for cloning mammals using telophase oocytes |
AU2003204830B2 (en) * | 1998-11-02 | 2007-08-30 | Gtc Biotherapeutics, Inc. | Transgenic and cloned mammals |
WO2000030436A1 (en) | 1998-11-19 | 2000-06-02 | Ppl Therapeutics (Scotland) Ltd. | Stabilisation of milk from transgenic animals |
US6258998B1 (en) | 1998-11-24 | 2001-07-10 | Infigen, Inc. | Method of cloning porcine animals |
US6700037B2 (en) | 1998-11-24 | 2004-03-02 | Infigen, Inc. | Method of cloning porcine animals |
AU780809B2 (en) * | 1999-01-13 | 2005-04-21 | Revivicor Inc | Double nuclear transfer method and results thereof |
GB9903805D0 (en) * | 1999-02-20 | 1999-04-14 | Univ Sheffield | Pluripotential cells-1 |
GB9903804D0 (en) * | 1999-02-20 | 1999-04-14 | Univ Sheffield | Pluripotential cells-2 |
EP2301947A3 (en) | 1999-02-26 | 2011-11-23 | Millennium Pharmaceuticals, Inc. | Secreted proteins and uses thereof |
US20020012660A1 (en) * | 1999-03-04 | 2002-01-31 | Alan Colman | Method of preparing a somatic cells for nuclear transfer |
DK1159415T3 (en) | 1999-03-04 | 2010-05-03 | Revivicor Inc | Genetic modification of somatic cells and their use |
BR0010588A (en) * | 1999-04-28 | 2003-07-22 | Univ Montreal | Nuclear transfer telophase enucleated oocytes |
EP1191840B1 (en) * | 1999-06-04 | 2007-01-17 | Istituto Zootecnico E Caseario per La Sardegna | Process for reconstructing a non-human animal embryo by nuclear transfer and preparing the animal therefrom. |
EP1194570A1 (en) | 1999-06-23 | 2002-04-10 | PPL Therapeutics (Scotland) Limited | Fusion proteins incorporating lysozyme |
CN1304445A (en) * | 1999-06-30 | 2001-07-18 | 黄禹锡 | Method for producing cloned tigers by employing inter-species nuclear transplantation technique |
CA2334953A1 (en) * | 1999-06-30 | 2001-01-04 | Woo-Suk Hwang | Method for producing human cloned embryos by employing inter-species nuclear transplantation technique |
EP1109891A4 (en) * | 1999-06-30 | 2004-11-17 | Hwang Woo Suk | Method for producing cloned cows |
US7291714B1 (en) | 1999-06-30 | 2007-11-06 | Millennium Pharmaceuticals, Inc. | Glycoprotein VI and uses thereof |
CN1362965A (en) * | 1999-06-30 | 2002-08-07 | 先进细胞技术公司 | cytoplasmic transfer to de-differentiate recipient cells |
HU228477B1 (en) | 1999-08-23 | 2013-03-28 | Dana Farber Cancer Inst Inc | Pd-1, a receptor for b7-4, and uses therefor |
WO2002064799A2 (en) | 1999-09-28 | 2002-08-22 | Transkaryotic Therapies, Inc. | Optimized messenger rna |
EP2275559A3 (en) | 1999-09-28 | 2011-03-23 | Shire Human Genetic Therapies, Inc. | Optimized messenger RNA |
DE60035205D1 (en) * | 1999-09-30 | 2007-07-26 | Gtc Biotherapeutics Inc | METHOD FOR PRODUCING NON-HUMAN CLAD AND TRANSGENIC MAMMALS |
US7820878B2 (en) * | 1999-11-19 | 2010-10-26 | Kyowa Hakko Kirin Co., Ltd. | Production of ungulates, preferably bovines that produce human immunoglobulins |
WO2001035735A1 (en) * | 1999-11-19 | 2001-05-25 | Hematech, Llc | Production of ungulates, preferably bovines that produce human immunoglobulins |
US7414170B2 (en) * | 1999-11-19 | 2008-08-19 | Kirin Beer Kabushiki Kaisha | Transgenic bovines capable of human antibody production |
US7074983B2 (en) * | 1999-11-19 | 2006-07-11 | Kirin Beer Kabushiki Kaisha | Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin |
KR100417566B1 (en) * | 1999-11-19 | 2004-02-05 | 한국생명공학연구원 | A mass production method of embryos by nuclear transfer using somatic cells |
US6635802B1 (en) * | 2000-01-10 | 2003-10-21 | The Texas A&M University System | Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors |
GB0001401D0 (en) * | 2000-01-22 | 2000-03-08 | Univ Edinburgh | Methods for amplifying genetic material and use thereof |
EP1356031B1 (en) | 2000-03-15 | 2011-07-27 | The University Of Georgia Research Foundation, Inc. | Effective nuclear reprogramming in mammals |
US20040033600A1 (en) | 2001-03-21 | 2004-02-19 | Palli Subba Reddy | Ecdysone receptor-based inducible gene expression system |
GB2360522A (en) | 2000-03-24 | 2001-09-26 | Geron Corp | A strategy for maintaining pregnancy |
WO2001088096A2 (en) * | 2000-05-15 | 2001-11-22 | Geron Corporation | Ovine tissue for xenotransplantation |
AU2001275346A1 (en) | 2000-06-05 | 2001-12-17 | General Hospital Corporation | Compositions, kits, and methods for identification and modulation of type i diabetes |
NZ522844A (en) | 2000-06-28 | 2005-02-25 | Brigham & Womens Hospital | PD-L2 molecules: novel PD-1 ligands and methods to identify compounds to modulate T cell activation |
AU2001278204A1 (en) * | 2000-08-01 | 2002-02-13 | Xy Genetics, Llc | Methods of selecting and cloning animals |
EP2044839A3 (en) | 2000-08-03 | 2009-07-01 | Therapeutic Human Polyclonals, Inc. | Production of humanized antibodies in transgenic animals |
WO2002014473A2 (en) | 2000-08-15 | 2002-02-21 | Board Of Trustees Of The University Of Illinois | Animals expressing exogenous igf-i in their milk |
AU2001291558A1 (en) * | 2000-09-06 | 2002-03-22 | Nexia Biotechnologies, Inc. | Generation of transgenic animals using nuclear transfer and oocytes at the germinal vesicle stage |
US8105825B2 (en) * | 2000-10-03 | 2012-01-31 | Intrexon Corporation | Multiple inducible gene regulation system |
FR2814642B1 (en) | 2000-10-03 | 2005-07-01 | Ass Pour Le Dev De La Rech En | TRANSGENIC MOUSE FOR THE TARGETED RECOMBINATION MEDIATED BY THE MODIFIED CRE-ER |
US20040029825A1 (en) * | 2001-10-03 | 2004-02-12 | Davies Christopher J | Methods of minimizing immunological rejection of a nuclear transfer fetus |
EP1356108A2 (en) | 2000-11-28 | 2003-10-29 | Wyeth | Expression analysis of fkbp nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer |
AU3650302A (en) | 2000-11-28 | 2002-06-11 | Wyeth Corp | Expression analysis of kiaa nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer |
DE60044301D1 (en) | 2000-12-22 | 2010-06-10 | Agronomique Inst Nat Rech | Position-independent and tissue-specific expression of a transgene in the milk of a transgenic animal |
US7491534B2 (en) | 2000-12-22 | 2009-02-17 | Kirin Holdings Kabushiki Kaisha | Methods for altering cell fate to generate T-cells specific for an antigen of interest |
WO2002051997A1 (en) * | 2000-12-22 | 2002-07-04 | Aurox Llc | Methods for cloning mammals using reprogrammed donor chromatin or donor cells |
US20020142397A1 (en) | 2000-12-22 | 2002-10-03 | Philippe Collas | Methods for altering cell fate |
EP1860199B1 (en) | 2001-01-12 | 2014-03-05 | Exelixis, Inc. | Modulating insulin receptor signaling |
US20050177883A1 (en) | 2001-01-30 | 2005-08-11 | Rebholtz Paul C. | Systems of transferring embryos and managing recipients |
AU2002250020A1 (en) * | 2001-02-02 | 2002-08-19 | Erik Forsberg | Method of cloning transgenic mammalian animals using pseudonuclei |
EP1572862B1 (en) * | 2001-02-20 | 2012-08-01 | Intrexon Corporation | Chimeric retinoid x receptors and their use in a novel ecdysone receptor-based inducible gene expression system |
US7309811B2 (en) | 2001-02-23 | 2007-12-18 | Elan Pharmaceuticals, Inc | Transgenic mice knockouts of BACE-1 |
JP2002262716A (en) * | 2001-03-07 | 2002-09-17 | National Agricultural Research Organization | Method for creating livestock individual using established cell line |
WO2002072762A2 (en) * | 2001-03-08 | 2002-09-19 | Advanced Cell Technology, Inc. | Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture |
AUPR363701A0 (en) | 2001-03-09 | 2001-04-05 | Garelag Pty Ltd | Method for the production of nuclear transfer embryos |
US8981061B2 (en) | 2001-03-20 | 2015-03-17 | Novo Nordisk A/S | Receptor TREM (triggering receptor expressed on myeloid cells) and uses thereof |
EP1370132A2 (en) * | 2001-03-21 | 2003-12-17 | Geron Corporation | Use of telomerase reverse transcriptase to create homozygous knockout animals |
US7126039B2 (en) * | 2001-03-21 | 2006-10-24 | Geron Corporation | Animal tissue with carbohydrate antigens compatible for human transplantation |
NZ528265A (en) | 2001-04-02 | 2005-10-28 | Wyeth Corp | Screening of compounds which modulate PD-1 signalling by testing for compounds that modulate phosphorylation of SHP-2, ERK1 or ERK-2, and PKC-theta |
WO2002078449A2 (en) * | 2001-04-02 | 2002-10-10 | Advanced Cell Technology, Inc. | Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells |
EP2258718A1 (en) | 2001-04-16 | 2010-12-08 | Wyeth Holdings Corporation | Streptococcus pneumoniae open reading frames encoding polypeptide antigens and uses thereof |
US20030092174A1 (en) * | 2001-05-14 | 2003-05-15 | Monika Liljedahl | Tissues or organs for use in xenotransplantation |
US20030153044A1 (en) * | 2001-05-14 | 2003-08-14 | Monika Liljedahl | Tissues or organs for use in xenotransplantation |
US20030101469A1 (en) * | 2001-07-09 | 2003-05-29 | Kenneth Bondioli | Cloned non-human mammals from contact inhibited donor cells |
JP4210737B2 (en) | 2001-07-12 | 2009-01-21 | ユニバーシティー オブ マサチューセッツ | In vivo production method of small interfering ribonucleic acid that mediates gene silencing |
EP1900815B1 (en) | 2001-07-12 | 2016-09-07 | University of Massachusetts | In vivo production of small interfering RNAs that mediate gene silencing |
AU2002326483A1 (en) * | 2001-07-31 | 2003-02-17 | Simon Eric Aspland | Genetically modified cows having reduced susceptibility to mad cow disease |
US20030211603A1 (en) * | 2001-08-14 | 2003-11-13 | Earp David J. | Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells |
AU2002352558A1 (en) | 2001-11-09 | 2003-05-26 | Dana-Farber Cancer Institute, Inc. | Pgc-1beta, a novel pgc-1 homologue and uses therefor |
US20050170506A1 (en) * | 2002-01-16 | 2005-08-04 | Primegen Biotech Llc | Therapeutic reprogramming, hybrid stem cells and maturation |
US20030134422A1 (en) * | 2002-01-16 | 2003-07-17 | Sayre Chauncey Bigelow | Stem cell maturation for all tissue lines |
US20050090004A1 (en) * | 2003-01-16 | 2005-04-28 | Sayre Chauncey B. | Stem cell maturation for all tissue lines |
US8106255B2 (en) | 2002-01-23 | 2012-01-31 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
KR100502695B1 (en) * | 2002-02-28 | 2005-07-25 | 한국생명공학연구원 | A method for mass production of cloned embryos for improving transgenesis in animals |
ATE531796T1 (en) * | 2002-03-21 | 2011-11-15 | Sangamo Biosciences Inc | METHODS AND COMPOSITIONS FOR USING ZINC FINGER ENDONUCLEASES TO IMPROVE HOMOLOGOUS RECOMBINATION |
GB0207299D0 (en) * | 2002-03-28 | 2002-05-08 | Franks Christopher R | Stem cell therapy |
CN1650003A (en) * | 2002-04-01 | 2005-08-03 | Gtc生物治疗学公司 | A method for selecting cell lines to be used for nuclear transfer in mammalian species |
US20030207448A1 (en) * | 2002-05-06 | 2003-11-06 | Revera Gregory Henry | Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo |
GB0211904D0 (en) * | 2002-05-23 | 2002-07-03 | Medical Res Council | Nuclear transfer |
AU2003247514A1 (en) * | 2002-06-11 | 2003-12-22 | Roy Ogle | Meningeal-derived stem cells |
US7612250B2 (en) * | 2002-07-29 | 2009-11-03 | Trustees Of Tufts College | Nuclear transfer embryo formation method |
US7361511B2 (en) | 2002-08-20 | 2008-04-22 | Millenium Pharmaceuticals, Inc. | Compositions, kits, and methods for identification, assessment, prevention, and therapy of cervical cancer |
AU2003298574B2 (en) * | 2002-09-05 | 2008-04-24 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
US7122361B2 (en) | 2002-10-10 | 2006-10-17 | Wyeth | Compositions employing a novel human kinase |
US20040077077A1 (en) * | 2002-10-18 | 2004-04-22 | Xiangzhong Yang | Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same |
EP1554385A2 (en) | 2002-10-24 | 2005-07-20 | Wyeth | Calcineurin-like human phoshphoesterase |
MXPA05004870A (en) | 2002-11-08 | 2005-11-04 | Hematech Llc | Transgenic ungulates having reduced prion protein activity and uses thereof. |
BR0316111A (en) | 2002-11-21 | 2005-09-13 | Wyeth Corp | Methods to diagnose rcc and other solid tumors |
US7297525B2 (en) | 2002-11-27 | 2007-11-20 | Wyeth | Composition employing a novel human kinase |
CN1735338A (en) * | 2002-12-10 | 2006-02-15 | Gtc生物治疗学公司 | Method and system for utilizing somatic cell nuclear transfer embryos as cell donors for additional nuclear transfer |
EP2474631B1 (en) | 2002-12-20 | 2014-02-12 | Celera Corporation | Genetic polymorphisms associated with myocardial infarction, methods of detection and uses thereof |
SI1581812T1 (en) | 2003-01-06 | 2008-10-31 | Wyeth Corp | Compositions and methods for diagnosing and treating colon cancers |
FR2855183B1 (en) | 2003-05-23 | 2005-08-12 | Agronomique Inst Nat Rech | PROCESS FOR CLONING THE RAT BY NUCLEAR TRANSFER |
ATE485394T1 (en) | 2003-06-02 | 2010-11-15 | Univ Massachusetts | METHODS AND COMPOSITIONS FOR IMPROVING THE EFFECTIVENESS AND SPECIFICITY OF FNAI |
EP1633767B1 (en) | 2003-06-02 | 2018-11-21 | University of Massachusetts | Methods and compositions for controlling efficacy of rna silencing |
US20120196370A1 (en) | 2010-12-03 | 2012-08-02 | Fyodor Urnov | Methods and compositions for targeted genomic deletion |
US8409861B2 (en) * | 2003-08-08 | 2013-04-02 | Sangamo Biosciences, Inc. | Targeted deletion of cellular DNA sequences |
US7888121B2 (en) | 2003-08-08 | 2011-02-15 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US11311574B2 (en) | 2003-08-08 | 2022-04-26 | Sangamo Therapeutics, Inc. | Methods and compositions for targeted cleavage and recombination |
JP2007502118A (en) | 2003-08-14 | 2007-02-08 | エクセリクシス, インク. | SULFs as modifiers of the β-catenin pathway and methods of use |
KR20060120652A (en) | 2003-10-07 | 2006-11-27 | 밀레니엄 파머슈티컬스 인코퍼레이티드 | Nucleic acid molecules and proteins for the identification, assessment, prevention, and therapy of ovarian cancer |
US7951532B2 (en) | 2003-11-12 | 2011-05-31 | Children's Hospital Medical Center | Method of screening a midkine modulating agent |
CA2921196C (en) | 2003-11-26 | 2019-03-05 | Celera Corporation | Single nucleotide polymorphisms associated with cardiovascular disorders and statin response, methods of detection and uses thereof |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US7420099B2 (en) * | 2004-04-22 | 2008-09-02 | Kirin Holdings Kabushiki Kaisha | Transgenic animals and uses thereof |
EP2423326B1 (en) | 2004-05-07 | 2014-01-08 | Celera Corporation | Genetic polymorphism associated with liver fibrosis methods of detection and uses thereof |
JP2006081542A (en) * | 2004-08-18 | 2006-03-30 | Institute Of Physical & Chemical Research | Method for creating clone mammal |
WO2006047312A2 (en) | 2004-10-22 | 2006-05-04 | Dana-Farber Cancer Institute, Inc. | COMPOSITIONS AND METHODS FOR MODULATING PGC-1β TO TREAT LIPID-RELATED DISEASES AND DISORDERS |
GB0426146D0 (en) | 2004-11-29 | 2004-12-29 | Bioxell Spa | Therapeutic peptides and method |
CN1274829C (en) | 2004-12-10 | 2006-09-13 | 四川大学华西医院 | Anti EB virus resulted tumour polypeptide, and its use and preparing method |
JP5590697B2 (en) | 2005-03-11 | 2014-09-17 | セレラ コーポレーション | Genetic polymorphism associated with coronary heart disease, detection method and use thereof |
US20060286666A1 (en) * | 2005-06-17 | 2006-12-21 | Kang Ji Y | Methods and apparatuses for culturing stem cell using biomaterial shell |
EP1924697A2 (en) * | 2005-06-29 | 2008-05-28 | Cameron Clokie | Production of bone morphogenic proteins (bmps) in transgenic mammals |
KR100733012B1 (en) | 2005-07-26 | 2007-06-28 | 재단법인서울대학교산학협력재단 | Cloned Caninds And Method For Producing Thereof |
AU2006292827B2 (en) | 2005-08-09 | 2013-02-14 | Revivicor, Inc. | Transgenic ungulates expressing CTLA4-IG and uses thereof |
US20100040636A1 (en) | 2005-09-09 | 2010-02-18 | The Johns Hopkins University | Manipulation of Regulatory T Cell and Dc Function By Targeting Neuritin Gene Using Antibodies, Agonists and Antagonists |
AU2006292224B2 (en) | 2005-09-19 | 2013-08-01 | Histogenics Corporation | Cell-support matrix and a method for preparation thereof |
US7799530B2 (en) | 2005-09-23 | 2010-09-21 | Celera Corporation | Genetic polymorphisms associated with cardiovascular disorders and drug response, methods of detection and uses thereof |
US7695911B2 (en) | 2005-10-26 | 2010-04-13 | Celera Corporation | Genetic polymorphisms associated with Alzheimer's Disease, methods of detection and uses thereof |
WO2007124019A2 (en) | 2006-04-21 | 2007-11-01 | Gtc Biotherapeutics, Inc. | Methods and products related to the transfer of molecules from blood to the mammary gland |
CN101506235B (en) | 2006-09-01 | 2012-07-25 | 人类多细胞株治疗学公司 | Enhanced expression of human or humanized immunoglobulin in non-human transgenic animals |
EP2639317B1 (en) | 2006-09-11 | 2015-03-04 | Celera Corporation | Genetic polymorphisms associated with psoriasis, methods of detection and uses thereof |
CA2915679C (en) | 2006-10-20 | 2017-12-12 | Celera Corporation | Genetic polymorphisms associated with venous thrombosis, methods of detection and uses thereof |
KR101398220B1 (en) * | 2006-10-30 | 2014-05-22 | 건국대학교 산학협력단 | Methods for producing transgenic embryos |
WO2008085891A1 (en) * | 2007-01-05 | 2008-07-17 | Worcester Polytechnic Institute | Oocyte spindle-associated factors improve somatic cell cloning |
KR100829426B1 (en) | 2007-01-17 | 2008-05-15 | 재단법인서울대학교산학협력재단 | Method for producing cloned dog |
US20080222745A1 (en) * | 2007-03-07 | 2008-09-11 | Utah State University | Colcemid-Treatment of Oocytes to enhance Nuclear Transfer Cloning |
KR100868245B1 (en) | 2007-05-29 | 2008-11-11 | 강원대학교산학협력단 | Effective producing method of cloned animal by demecolcine treatment |
EP2214708A4 (en) | 2007-10-26 | 2011-01-12 | Centocor Ortho Biotech Inc | Vectors, host cells, and methods of production and uses |
US8039212B2 (en) | 2007-11-05 | 2011-10-18 | Celera Corporation | Genetic polymorphisms associated with liver fibrosis, methods of detection and uses thereof |
JP2011508600A (en) | 2008-01-04 | 2011-03-17 | ソウル ナショナル ユニバーシティ インダストリー ファウンデーション | Method for producing transformed replicated dogs |
US20090221620A1 (en) | 2008-02-20 | 2009-09-03 | Celera Corporation | Gentic polymorphisms associated with stroke, methods of detection and uses thereof |
EP2265730B1 (en) | 2008-03-12 | 2018-06-06 | The Rockefeller University | Methods and compositions for translational profiling and molecular phenotyping |
US8288094B2 (en) | 2008-04-09 | 2012-10-16 | Institut Pasteur | APOBEC3 mediated DNA editing |
EP2987498B1 (en) | 2008-05-02 | 2017-07-05 | Laboratoire Français du Fractionnement et des Biotechnologies | Treatment of bleeding with low half-life fibrinogen |
KR101642790B1 (en) | 2008-07-07 | 2016-07-27 | 다카라 바이오 가부시키가이샤 | Method for production of pluripotent stem cell |
EP3093351B1 (en) | 2008-07-09 | 2018-04-18 | Celera Corporation | Genetic polymorphisms associated with cardiovascular diseases, methods of detection and uses thereof |
US9181315B2 (en) | 2009-01-08 | 2015-11-10 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for induced brown fat differentiation |
US8722964B2 (en) | 2009-04-23 | 2014-05-13 | Transposagen Biopharmaceuticals, Inc. | Genetically engineered or transgenic rats exhibiting a cancer phenotype due to a disruption of germline tumor suppressor genes |
EP2267153A1 (en) | 2009-05-26 | 2010-12-29 | Université Claude Bernard Lyon 1 | Identification of netrin-1 receptor unc5c gene mutation in solid cancers |
US9314005B2 (en) | 2009-07-01 | 2016-04-19 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for severe combined immunodeficiency (SCID) |
US8558055B2 (en) | 2009-07-24 | 2013-10-15 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat comprising a cytokine gene disruption and exhibiting a greater susceptibility to a cytokine-mediated autoimmune and/or inflammatory disease |
WO2011014721A2 (en) | 2009-07-30 | 2011-02-03 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for pharmacokinetics |
US20110035816A1 (en) | 2009-08-05 | 2011-02-10 | Ostertag Eric M | Genetically Modified Rat Models for Drug Metabolism |
WO2011022634A2 (en) | 2009-08-20 | 2011-02-24 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for pain |
US9420770B2 (en) | 2009-12-01 | 2016-08-23 | Indiana University Research & Technology Corporation | Methods of modulating thrombocytopenia and modified transgenic pigs |
US10429384B2 (en) | 2010-01-22 | 2019-10-01 | Dana-Farber Cancer Institute, Inc. | Compositions, kits, and methods for identification, assessment, prevention, and therapy of metabolic disorders |
TWI518325B (en) | 2010-02-04 | 2016-01-21 | 自治醫科大學 | Identification, assessment, and therapy of cancers with innate or acquired resistance to alk inhibitors |
BR112012024565B1 (en) | 2010-03-26 | 2022-02-08 | Trustees Of Dartmouth College | FUSION PROTEIN VIEWED ISOLATED OR RECOMBINANT MULTIMERIC IMMUNOSUPPRESSANT AND COMPOSITION |
US10745467B2 (en) | 2010-03-26 | 2020-08-18 | The Trustees Of Dartmouth College | VISTA-Ig for treatment of autoimmune, allergic and inflammatory disorders |
US20150231215A1 (en) | 2012-06-22 | 2015-08-20 | Randolph J. Noelle | VISTA Antagonist and Methods of Use |
US8383793B2 (en) | 2010-04-15 | 2013-02-26 | St. Jude Children's Research Hospital | Methods and compositions for the diagnosis and treatment of cancer resistant to anaplastic lymphoma kinase (ALK) kinase inhibitors |
US20110269735A1 (en) | 2010-04-19 | 2011-11-03 | Celera Corporation | Genetic polymorphisms associated with statin response and cardiovascular diseases, methods of detection and uses thereof |
WO2012020077A1 (en) | 2010-08-11 | 2012-02-16 | Inserm (Institut Mational De La Santé Et De La Recherche Médicale) | Methods of growing an embryo to a blastocyst stage of development |
US20120108651A1 (en) | 2010-11-02 | 2012-05-03 | Leiden University Medical Center (LUMC) Acting on Behalf of Academic Hospital Leiden (AZL) | Genetic polymorphisms associated with venous thrombosis and statin response, methods of detection and uses thereof |
US8748178B2 (en) | 2010-11-23 | 2014-06-10 | The New York Stem Cell Foundation | Method for producing pluripotent stem cells |
ES2680636T3 (en) | 2011-02-14 | 2018-09-10 | Revivicor Inc. | Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof |
WO2012129198A1 (en) | 2011-03-23 | 2012-09-27 | Transposagen Biopharmaceuticals, Inc. | Genetically modified rat models for obesity and diabetes |
KR20140034229A (en) | 2011-05-16 | 2014-03-19 | 더 큐레이터스 오브 더 유니버시티 오브 미주리 | Porcine reproductive and respiratory syndrome virus resistant animals |
WO2013039996A1 (en) | 2011-09-13 | 2013-03-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for brown fat induction and activity using fndc5 |
CN102391989A (en) * | 2011-10-12 | 2012-03-28 | 扬州大学 | Exact analysis inspection method for transgenic animal recombinant product |
LT2814844T (en) | 2012-02-15 | 2017-10-25 | Novo Nordisk A/S | Antibodies that bind and block triggering receptor expressed on myeloid cells-1 (trem-1) |
US9550830B2 (en) | 2012-02-15 | 2017-01-24 | Novo Nordisk A/S | Antibodies that bind and block triggering receptor expressed on myeloid cells-1 (TREM-1) |
CN108530535B (en) | 2012-02-15 | 2021-02-26 | 诺和诺德股份有限公司 | Antibody binding to peptidoglycan-recognizing protein 1 |
EP2864352B1 (en) | 2012-06-22 | 2018-08-08 | The Trustees Of Dartmouth College | Novel vista-ig constructs and the use of vista-ig for treatment of autoimmune, allergic and inflammatory disorders |
US9890215B2 (en) | 2012-06-22 | 2018-02-13 | King's College London | Vista modulators for diagnosis and treatment of cancer |
CN109793893B (en) | 2012-09-07 | 2023-05-26 | 达特茅斯大学理事会 | VISTA modulators for diagnosis and treatment of cancer |
US20140115728A1 (en) | 2012-10-24 | 2014-04-24 | A. Joseph Tector | Double knockout (gt/cmah-ko) pigs, organs and tissues |
CA2900909A1 (en) | 2013-02-13 | 2014-08-21 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Highly galactosylated anti-tnf-.alpha. antibodies and uses thereof |
CN105263319A (en) | 2013-02-13 | 2016-01-20 | 法国化学与生物科技实验室 | Proteins with modified glycosylation and methods of production thereof |
PL2993234T3 (en) | 2013-04-30 | 2019-04-30 | Univ Konkuk Ind Coop Corp | Cmp-acetylneuraminic acid hydroxylase targeting vector, transgenic animal for xenotransplantation introduced with the vector, and method of manufacturing the same |
KR102534681B1 (en) | 2013-12-24 | 2023-05-18 | 잔센파마슈티카엔.브이. | Anti-vista antibodies and fragments |
US11014987B2 (en) | 2013-12-24 | 2021-05-25 | Janssen Pharmaceutics Nv | Anti-vista antibodies and fragments, uses thereof, and methods of identifying same |
WO2015191881A2 (en) | 2014-06-11 | 2015-12-17 | Green Kathy A | Use of vista agonists and antagonists to suppress or enhance humoral immunity |
SG10201913702WA (en) | 2014-07-17 | 2020-03-30 | Novo Nordisk As | Site directed mutagenesis of trem-1 antibodies for decreasing viscosity |
US10077420B2 (en) | 2014-12-02 | 2018-09-18 | Histogenics Corporation | Cell and tissue culture container |
AU2015357463B2 (en) | 2014-12-05 | 2021-10-07 | Immunext, Inc. | Identification of VSIG8 as the putative vista receptor and its use thereof to produce vista/VSIG8 modulators |
SG11201703931QA (en) | 2014-12-09 | 2017-06-29 | Regeneron Pharma | Non-human animals having a humanized cluster of differentiation 274 gene |
CN104823921A (en) * | 2015-05-21 | 2015-08-12 | 蒙汉勤 | Feeding technology of mini fragrant pigs for human body burnt skin transplantation and medical experiments |
BR112017027870A2 (en) | 2015-06-24 | 2018-08-28 | Janssen Pharmaceutica Nv | antibodies and anti-sight fragments |
US10827730B2 (en) | 2015-08-06 | 2020-11-10 | The Curators Of The University Of Missouri | Pathogen-resistant animals having modified CD163 genes |
AU2016343978A1 (en) | 2015-10-29 | 2018-05-17 | Dana-Farber Cancer Institute, Inc. | Methods for identification, assessment, prevention, and treatment of metabolic disorders using PM20D1 and N-lipidated amino acids |
BR112018016461A2 (en) | 2016-02-12 | 2019-10-01 | Janssen Pharmaceutica Nv | antibodies and anti-sight fragments, their uses and their identification methods |
CR20180537A (en) | 2016-04-15 | 2019-03-04 | Immunext Inc | ANTIHUMAN VIEW ANTIBODIES AND THEIR USE |
CN111936520A (en) | 2018-04-02 | 2020-11-13 | 百时美施贵宝公司 | anti-TREM-1 antibodies and uses thereof |
JP2021520781A (en) | 2018-04-06 | 2021-08-26 | チルドレンズ メディカル センター コーポレーションChildren’S Medical Center Corporation | Compositions and Methods for Somatic Cell Reprogramming and Imprinting Modulation |
US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
WO2020041360A1 (en) | 2018-08-21 | 2020-02-27 | Quidel Corporation | Dbpa antibodies and uses thereof |
EP4077376A2 (en) | 2019-12-19 | 2022-10-26 | Quidel Corporation | Monoclonal antibody fusions |
KR20230107335A (en) | 2020-11-20 | 2023-07-14 | 레비비코르 인코포레이션 | Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation |
US20230255185A1 (en) | 2021-09-20 | 2023-08-17 | Revivicor, Inc. | Multitransgenic pigs comprising ten genetic modifications for xenotransplantation |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR890003947B1 (en) * | 1985-12-11 | 1989-10-13 | 가부시기가이샤 시마즈세이사구쇼 | Apparatus for cell fusion |
US5057420A (en) * | 1987-06-05 | 1991-10-15 | Granada Biosciences, Inc. | Bovine nuclear transplantation |
GB8714426D0 (en) * | 1987-06-19 | 1987-07-22 | Agricultural & Food Res | Culture technique |
CA2059199A1 (en) * | 1992-01-10 | 1993-07-11 | Steen Willadsen | Method for producing mature reconstituted mammalian eggs by nuclear transplantation |
GB2265909B (en) * | 1992-04-01 | 1996-03-20 | Inst Of Animal Physiology And | Fusion of donor nucleus with recipient cytoplast in the absence of calcium ion |
ATE234088T1 (en) * | 1992-09-22 | 2003-03-15 | Us Gov Health & Human Serv | TAXOL FOR THE TREATMENT OF LYMPHOMAS AND BREAST CANCER AND TO REDUCE MULTI-DRUG RESISTANCE TO TAXOL |
CA2092258A1 (en) * | 1992-12-30 | 1994-07-01 | Steven L. Stice | Synchronized donor cells for bovine nuclear transfer |
US5496720A (en) * | 1993-02-10 | 1996-03-05 | Susko-Parrish; Joan L. | Parthenogenic oocyte activation |
US5523226A (en) | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
PT711158E (en) * | 1993-07-29 | 2004-04-30 | Us Gov Health & Human Serv | METHOD FOR TREATING ATHEROSCLEROSIS OR RESTENING USING A MICROTUBLE STABILIZING AGENT |
AU1439295A (en) * | 1993-12-17 | 1995-07-03 | Abs Global, Inc. | Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates |
DE69430014D1 (en) * | 1993-12-23 | 2002-04-04 | Infigen Inc | EMBRYONIC STEM CELLS of ungulates AS CORE DONATORS AND CORE TRANSFER TECHNIQUES FOR THE PRODUCTION OF CHIMERIC AND TRANSGENIC ANIMALS |
GB9417831D0 (en) * | 1994-09-05 | 1994-10-26 | Biotech & Biolog Scien Res | Biological manipulation |
GB9517780D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
US7307198B2 (en) | 1995-08-31 | 2007-12-11 | Roslin Institute | Ungulates produced by nuclear transfer of G1 cells |
GB9517779D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
US6215041B1 (en) | 1997-01-10 | 2001-04-10 | University Of Mmassachusetts | Cloning using donor nuclei from a non-quiesecent somatic cells |
US6235969B1 (en) | 1997-01-10 | 2001-05-22 | University Of Massachusetts | Cloning pigs using donor nuclei from non-quiescent differentiated cells |
US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
WO1999005266A2 (en) * | 1997-07-26 | 1999-02-04 | Wisconsin Alumni Research Foundation | Trans-species nuclear transfer |
US6225243B1 (en) * | 1998-08-03 | 2001-05-01 | Bba Nonwovens Simpsonville, Inc. | Elastic nonwoven fabric prepared from bi-component filaments |
WO2000022098A1 (en) * | 1998-10-12 | 2000-04-20 | Geron Bio-Med Limited | Porcine oocytes with improved developmental competence |
US20050156424A1 (en) * | 2000-02-29 | 2005-07-21 | Thyssenkrupp Presta Ag | Steering column and manufacturing method thereof |
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