CA2377071A1 - Methods and apparatus for performing array microcrystallizations - Google Patents

Methods and apparatus for performing array microcrystallizations Download PDF

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Publication number
CA2377071A1
CA2377071A1 CA002377071A CA2377071A CA2377071A1 CA 2377071 A1 CA2377071 A1 CA 2377071A1 CA 002377071 A CA002377071 A CA 002377071A CA 2377071 A CA2377071 A CA 2377071A CA 2377071 A1 CA2377071 A1 CA 2377071A1
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Prior art keywords
microcrystallizations
array
molecule
methods
performing array
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CA002377071A
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French (fr)
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CA2377071C (en
Inventor
Bernard D. Santarsiero
Peter G. Schultz
Derek T. Yegian
Robert A. Nordmeyer
William F. Kolbe
Donald C. Uber
Raymond C. Stevens
Joseph M. Jaklevic
Earl W. Cornell
Jian Jin
Arthur L. Jones
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University of California
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The Regents Of The University Of California
Bernard D. Santarsiero
Peter G. Schultz
Derek T. Yegian
Robert A. Nordmeyer
William F. Kolbe
Donald C. Uber
Raymond C. Stevens
Joseph M. Jaklevic
Earl W. Cornell
Jian Jin
Arthur L. Jones
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Application filed by The Regents Of The University Of California, Bernard D. Santarsiero, Peter G. Schultz, Derek T. Yegian, Robert A. Nordmeyer, William F. Kolbe, Donald C. Uber, Raymond C. Stevens, Joseph M. Jaklevic, Earl W. Cornell, Jian Jin, Arthur L. Jones filed Critical The Regents Of The University Of California
Publication of CA2377071A1 publication Critical patent/CA2377071A1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
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    • C30—CRYSTAL GROWTH
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    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
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Abstract

A method is provided for performing array microcrystallizations to determine suitable crystallization conditions for a molecule. The method comprises forming an array of microcrystallizations, each microcrystallization comprising a drop containing a molecule to be crystallized, the drop having a volume of less than 1 µL;
storing the array of microcrystallizations under conditions suitable for molecule crystals to form in the drops in the array; and detecting molecule crystal formation in the drops.
CA002377071A 1999-06-18 2000-06-07 Methods and apparatus for performing array microcrystallizations Expired - Fee Related CA2377071C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/336,134 US6296673B1 (en) 1999-06-18 1999-06-18 Methods and apparatus for performing array microcrystallizations
US09/336,134 1999-06-18
PCT/US2000/015711 WO2000078445A1 (en) 1999-06-18 2000-06-07 Methods and apparatus for performing array microcrystallizations

Publications (2)

Publication Number Publication Date
CA2377071A1 true CA2377071A1 (en) 2000-12-28
CA2377071C CA2377071C (en) 2007-09-18

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CA002377071A Expired - Fee Related CA2377071C (en) 1999-06-18 2000-06-07 Methods and apparatus for performing array microcrystallizations

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US (2) US6296673B1 (en)
EP (1) EP1204467A1 (en)
JP (2) JP2003502652A (en)
KR (1) KR20020036961A (en)
AU (1) AU777212B2 (en)
CA (1) CA2377071C (en)
IL (1) IL146628A (en)
IS (1) IS6176A (en)
MX (1) MXPA01012796A (en)
RU (1) RU2002100812A (en)
SG (2) SG130024A1 (en)
WO (1) WO2000078445A1 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6833242B2 (en) * 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US7250305B2 (en) * 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US20070281359A1 (en) * 1999-04-06 2007-12-06 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US7214540B2 (en) 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7244396B2 (en) 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US20030022383A1 (en) * 1999-04-06 2003-01-30 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US6899137B2 (en) * 1999-06-28 2005-05-31 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US8709153B2 (en) 1999-06-28 2014-04-29 California Institute Of Technology Microfludic protein crystallography techniques
GB2352283A (en) 1999-06-28 2001-01-24 California Inst Of Techn Microfabricated valves, pumps, mirror array and refracting structure
US7195670B2 (en) 2000-06-27 2007-03-27 California Institute Of Technology High throughput screening of crystallization of materials
US20080277007A1 (en) * 1999-06-28 2008-11-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6929030B2 (en) * 1999-06-28 2005-08-16 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7306672B2 (en) 2001-04-06 2007-12-11 California Institute Of Technology Microfluidic free interface diffusion techniques
US7052545B2 (en) * 2001-04-06 2006-05-30 California Institute Of Technology High throughput screening of crystallization of materials
US8052792B2 (en) 2001-04-06 2011-11-08 California Institute Of Technology Microfluidic protein crystallography techniques
US7459022B2 (en) * 2001-04-06 2008-12-02 California Institute Of Technology Microfluidic protein crystallography
US7144616B1 (en) 1999-06-28 2006-12-05 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US7244402B2 (en) * 2001-04-06 2007-07-17 California Institute Of Technology Microfluidic protein crystallography
US7217321B2 (en) * 2001-04-06 2007-05-15 California Institute Of Technology Microfluidic protein crystallography techniques
US8550119B2 (en) * 1999-06-28 2013-10-08 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6818060B2 (en) 1999-08-02 2004-11-16 Emerald Biostructures, Inc. Robot for mixing crystallization trial matrices
US20050089923A9 (en) * 2000-01-07 2005-04-28 Levinson Douglas A. Method and system for planning, performing, and assessing high-throughput screening of multicomponent chemical compositions and solid forms of compounds
US20070020662A1 (en) * 2000-01-07 2007-01-25 Transform Pharmaceuticals, Inc. Computerized control of high-throughput experimental processing and digital analysis of comparative samples for a compound of interest
US20020048610A1 (en) * 2000-01-07 2002-04-25 Cima Michael J. High-throughput formation, identification, and analysis of diverse solid-forms
US20070021929A1 (en) * 2000-01-07 2007-01-25 Transform Pharmaceuticals, Inc. Computing methods for control of high-throughput experimental processing, digital analysis, and re-arraying comparative samples in computer-designed arrays
US20050095696A9 (en) * 2000-01-07 2005-05-05 Lemmo Anthony V. Apparatus and method for high-throughput preparation and characterization of compositions
US20050118637A9 (en) * 2000-01-07 2005-06-02 Levinson Douglas A. Method and system for planning, performing, and assessing high-throughput screening of multicomponent chemical compositions and solid forms of compounds
US20040252299A9 (en) * 2000-01-07 2004-12-16 Lemmo Anthony V. Apparatus and method for high-throughput preparation and spectroscopic classification and characterization of compositions
US7108970B2 (en) * 2000-01-07 2006-09-19 Transform Pharmaceuticals, Inc. Rapid identification of conditions, compounds, or compositions that inhibit, prevent, induce, modify, or reverse transitions of physical state
AU2001240040A1 (en) * 2000-03-03 2001-09-17 California Institute Of Technology Combinatorial array for nucleic acid analysis
US6409832B2 (en) * 2000-03-31 2002-06-25 Micronics, Inc. Protein crystallization in microfluidic structures
US7279146B2 (en) * 2003-04-17 2007-10-09 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US20050118073A1 (en) * 2003-11-26 2005-06-02 Fluidigm Corporation Devices and methods for holding microfluidic devices
US8105553B2 (en) 2004-01-25 2012-01-31 Fluidigm Corporation Crystal forming devices and systems and methods for using the same
US7867763B2 (en) 2004-01-25 2011-01-11 Fluidigm Corporation Integrated chip carriers with thermocycler interfaces and methods of using the same
GB0008563D0 (en) * 2000-04-07 2000-05-24 Cambridge Discovery Chemistry Investigating different physical and/or chemical forms of materials
WO2001092293A2 (en) * 2000-05-31 2001-12-06 Universiteit Leiden Methods for identifying crystallization conditions for biomolecules
US7351376B1 (en) * 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
WO2002000343A2 (en) * 2000-06-27 2002-01-03 Fluidigm Corporation A microfluidic design automation method and system
EP1172646A1 (en) * 2000-07-13 2002-01-16 Universiteit Leiden Screening crystallisation conditions of organic compounds
EP2299256A3 (en) * 2000-09-15 2012-10-10 California Institute Of Technology Microfabricated crossflow devices and methods
US20030048341A1 (en) * 2000-09-25 2003-03-13 Mutz Mitchell W. High-throughput biomolecular crystallization and biomolecular crystal screening
US6808934B2 (en) * 2000-09-25 2004-10-26 Picoliter Inc. High-throughput biomolecular crystallization and biomolecular crystal screening
US20020037359A1 (en) 2000-09-25 2002-03-28 Mutz Mitchell W. Focused acoustic energy in the preparation of peptide arrays
AU2002224336B2 (en) * 2000-09-25 2007-01-04 Picoliter Inc. Acoustic ejection of fluids from a plurality of reservoirs
US7678547B2 (en) 2000-10-03 2010-03-16 California Institute Of Technology Velocity independent analyte characterization
US7258774B2 (en) * 2000-10-03 2007-08-21 California Institute Of Technology Microfluidic devices and methods of use
US7097809B2 (en) * 2000-10-03 2006-08-29 California Institute Of Technology Combinatorial synthesis system
US6402837B1 (en) * 2000-10-30 2002-06-11 Abraham Shtrahman Apparatus and method of preparation for automated high output biopolymer crystallization via vapor diffusion sitting drop and micro-batch techniques
US6637473B2 (en) * 2000-10-30 2003-10-28 Robodesign International, Inc. Automated storage and retrieval device and method
US7232109B2 (en) * 2000-11-06 2007-06-19 California Institute Of Technology Electrostatic valves for microfluidic devices
WO2002060582A2 (en) * 2000-11-16 2002-08-08 Fluidigm Corporation Microfluidic devices for introducing and dispensing fluids from microfluidic systems
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
US20050196785A1 (en) * 2001-03-05 2005-09-08 California Institute Of Technology Combinational array for nucleic acid analysis
US6869792B2 (en) * 2001-03-16 2005-03-22 Irm, Llc Method and apparatus for performing multiple processing steps on a sample in a single vessel
US6913732B2 (en) 2001-03-19 2005-07-05 Corning Incorporated Microplate for performing crystallography studies and methods for making and using such microplates
US6659142B2 (en) 2001-03-27 2003-12-09 Irm, Llc Apparatus and method for preparing fluid mixtures
GB0108289D0 (en) * 2001-04-03 2001-05-23 Imp College Innovations Ltd Crystal optimisation technique
US7670429B2 (en) 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
ATE500051T1 (en) 2001-04-06 2011-03-15 Fluidigm Corp POLYMER SURFACE MODIFICATION
US6960437B2 (en) 2001-04-06 2005-11-01 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US6752922B2 (en) * 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US20060129329A1 (en) * 2001-04-09 2006-06-15 Kobylecki Ryszard J Investigating different physical and/or chemical forms of materials
WO2002089943A1 (en) * 2001-05-07 2002-11-14 The Regents Of The University Of California Method for screening microcrystallizations for crystal formation
DE50205587D1 (en) * 2001-06-18 2006-04-06 Greiner Bio One Gmbh REACTION VESSEL FOR THE PREPARATION OF SAMPLES
US20030119060A1 (en) * 2001-08-10 2003-06-26 Desrosiers Peter J. Apparatuses and methods for creating and testing pre-formulations and systems for same
US7075162B2 (en) * 2001-08-30 2006-07-11 Fluidigm Corporation Electrostatic/electrostrictive actuation of elastomer structures using compliant electrodes
US20030106492A1 (en) * 2001-09-07 2003-06-12 Douglas Levinson Apparatus and method for high-throughput preparation, visualization and screening of compositions
US7153699B2 (en) * 2001-12-21 2006-12-26 Cytonome, Inc. Microfabricated two-pin system for biomolecule crystallization
US20070054408A1 (en) * 2001-09-25 2007-03-08 Cytonome, Inc. Microfabricated two-pin system for biomolecule crystallization
WO2003031066A1 (en) 2001-10-11 2003-04-17 California Institute Of Technology Devices utilizing self-assembled gel and method of manufacture
WO2003035208A1 (en) * 2001-10-23 2003-05-01 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
EP1462454A4 (en) * 2001-12-11 2007-05-23 Mitsubishi Rayon Co Array for crystallizing protein, device for crystallizing protein and method of screening protein crystallization using the same
US7332029B2 (en) * 2002-01-18 2008-02-19 Neuro Probe Incorporated Crystal forming apparatus and method for using same
JP2005521425A (en) 2002-04-01 2005-07-21 フルイディグム コーポレイション Microfluidic particle analysis system
US7312085B2 (en) * 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7901939B2 (en) 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
WO2004038363A2 (en) * 2002-05-09 2004-05-06 The University Of Chicago Microfluidic device and method for pressure-driven plug transport and reaction
AU2003247689A1 (en) * 2002-07-05 2004-01-23 Aventis Pharmaceuticals Inc. Apparatus and method for use in solid phase chemical synthesis
EP1526905B1 (en) * 2002-07-30 2011-06-08 Kurt Dr. Hoffmann Protein crystallisation method
US6849459B2 (en) * 2002-09-09 2005-02-01 Cytonome, Inc. Microfluidic chip for biomolecule crystallization
US7143785B2 (en) * 2002-09-25 2006-12-05 California Institute Of Technology Microfluidic large scale integration
US8220494B2 (en) 2002-09-25 2012-07-17 California Institute Of Technology Microfluidic large scale integration
EP1546412B1 (en) 2002-10-02 2014-05-21 California Institute Of Technology Microfluidic nucleic acid analysis
US20100210027A9 (en) * 2002-11-04 2010-08-19 Hongming Chen Method for determining effect of preformulation forms on their dissolution rates
ATE397177T1 (en) * 2002-12-04 2008-06-15 Spinx Inc DEVICES AND METHODS FOR THE PROGRAMMABLE MICROHANDLING OF FLUIDS
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
CA2521171C (en) 2003-04-03 2013-05-28 Fluidigm Corp. Microfluidic devices and methods of using same
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
JP3753134B2 (en) * 2003-04-28 2006-03-08 松下電器産業株式会社 Protein crystallization condition screening device
EP1636017A2 (en) 2003-05-20 2006-03-22 Fluidigm Corporation Method and system for microfluidic device and imaging thereof
US7300520B2 (en) * 2003-07-09 2007-11-27 The Trustees Of Columbia University In The City Of New York Crystallization reagent matrices and related methods and kits
AU2004261655A1 (en) * 2003-07-28 2005-02-10 Fluidigm Corporation Image processing method and system for microfluidic devices
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7407799B2 (en) * 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US20060024751A1 (en) * 2004-06-03 2006-02-02 Fluidigm Corporation Scale-up methods and systems for performing the same
US9477233B2 (en) * 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7655470B2 (en) * 2004-10-29 2010-02-02 University Of Chicago Method for manipulating a plurality of plugs and performing reactions therein in microfluidic systems
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP2364774A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US20070185346A1 (en) * 2006-02-03 2007-08-09 Vaidya Niteen A Kit for automated resolving agent selection and method thereof
US7815868B1 (en) 2006-02-28 2010-10-19 Fluidigm Corporation Microfluidic reaction apparatus for high throughput screening
WO2007123908A2 (en) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US7763471B2 (en) * 2006-04-18 2010-07-27 Advanced Liquid Logic, Inc. Method of electrowetting droplet operations for protein crystallization
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080014589A1 (en) 2006-05-11 2008-01-17 Link Darren R Microfluidic devices and methods of use thereof
JP4884158B2 (en) * 2006-06-26 2012-02-29 矢崎総業株式会社 Lever type connector
EP2077912B1 (en) 2006-08-07 2019-03-27 The President and Fellows of Harvard College Fluorocarbon emulsion stabilizing surfactants
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
JP2009139316A (en) * 2007-12-10 2009-06-25 Furukawa Co Ltd Dispensing apparatus
EP2315629B1 (en) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Droplet libraries
EP2168646A1 (en) * 2008-09-30 2010-03-31 Qiagen GmbH Method of loading a crystallization device
JP5651125B2 (en) 2008-12-10 2015-01-07 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド MEK mutations that confer resistance to MEK inhibitors
EP2411148B1 (en) 2009-03-23 2018-02-21 Raindance Technologies, Inc. Manipulation of microfluidic droplets
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
CA2789425C (en) 2010-02-12 2020-04-28 Raindance Technologies, Inc. Digital analyte analysis with polymerase error correction
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
MX360932B (en) 2010-02-25 2018-11-21 Dana Farber Cancer Inst Inc Braf mutations conferring resistance to braf inhibitors.
US20130143911A1 (en) 2010-06-09 2013-06-06 Dana-Farber Cancer Institute, Inc. MEK1 Mutation Conferring Resistance to RAF and MEK Inhibitors
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
EP3190067B1 (en) * 2010-11-11 2018-07-11 Nestec S.A. Beverage production machine and system for the preparation of a beverage
WO2012109600A2 (en) 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
EP3736281A1 (en) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Compositions and methods for molecular labeling
US10175183B2 (en) 2011-03-31 2019-01-08 Kunimine Industries Co., Ltd. Agent for searching for protein crystallization conditions and method of searching for protein crystallization conditions
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
WO2014045254A2 (en) 2012-09-23 2014-03-27 Erasmus University Medical Center Rotterdam Human betacoronavirus lineage c and identification of n-terminal dipeptidyl peptidase as its virus receptor
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US9901844B2 (en) * 2014-03-07 2018-02-27 University of Pittsburgh—of the Commonwealth System of Higher Education Nano seeding tools to generate nanometer size crystallization seeds
US9869648B2 (en) * 2014-06-26 2018-01-16 The Board Of Trustees Of The Leland Stanford Junior University High density grids
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4263010A (en) 1979-10-31 1981-04-21 University Patents, Inc. Control method and apparatus for crystallizer process control
JPS62106000A (en) 1985-10-30 1987-05-16 Fujitsu Ltd Apparatus for automatic production of biopolymer crystal
US4668584A (en) 1985-12-23 1987-05-26 General Electric Company Method for forming 2-D crystals of proteins and macromolecules
US4833233A (en) 1987-08-20 1989-05-23 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Human serum albumin crystals and method of preparation
US4919899A (en) 1988-02-29 1990-04-24 Herrmann Frederick T Crystal growth apparatus
US4886646A (en) 1988-03-23 1989-12-12 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Hanging drop crystal growth apparatus and method
US5096676A (en) 1989-01-27 1992-03-17 Mcpherson Alexander Crystal growing apparatus
US5078975A (en) 1990-12-18 1992-01-07 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Drop deployment system for crystal growth apparatus
US5221410A (en) 1991-10-09 1993-06-22 Schering Corporation Crystal forming device
WO1993007311A1 (en) 1991-10-09 1993-04-15 Schering Corporation Crystal forming device and automated crystallization system
US5581476A (en) * 1993-01-28 1996-12-03 Amgen Inc. Computer-based methods and articles of manufacture for preparing G-CSF analogs
RU2041263C1 (en) * 1993-08-11 1995-08-09 Геннадий Моисеевич Ершов Method and apparatus for microdosing and dispensing of aqueous solutions onto carrier
US5419278A (en) 1994-05-25 1995-05-30 Carter; Daniel C. Vapor equilibration tray for growing protein crystals
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5643540A (en) 1995-02-27 1997-07-01 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Protein crystal growth apparatus for microgravitiy
US5641681A (en) 1995-04-17 1997-06-24 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Device and method for screening crystallization conditions in solution crystal growth
US5872010A (en) 1995-07-21 1999-02-16 Northeastern University Microscale fluid handling system
US5658802A (en) * 1995-09-07 1997-08-19 Microfab Technologies, Inc. Method and apparatus for making miniaturized diagnostic arrays
US6083762A (en) * 1996-05-31 2000-07-04 Packard Instruments Company Microvolume liquid handling system
US6024925A (en) * 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US5985214A (en) * 1997-05-16 1999-11-16 Aurora Biosciences Corporation Systems and methods for rapidly identifying useful chemicals in liquid samples
US5873394A (en) 1997-07-02 1999-02-23 Cyberlab, Inc. Automated sample preparation workstation for the vapor diffusion method of crystallization and method of preparation
US6130105A (en) * 1997-08-28 2000-10-10 Applied Materials, Inc. Deposition rate control on wafers with varying characteristics
US6057159A (en) 1997-12-12 2000-05-02 Vertex Pharmaceuticals Incorporated Processes for identifying a solvent condition suitable for determining a biophysical property of a protein
US6063339A (en) * 1998-01-09 2000-05-16 Cartesian Technologies, Inc. Method and apparatus for high-speed dot array dispensing
US6039804A (en) * 1998-09-09 2000-03-21 Emerald Biostructures, Inc. Crystallization tray
MXPA01009999A (en) 1999-04-06 2003-07-14 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth.

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