CA2518933A1 - Method of detecting colon cancer marker - Google Patents

Method of detecting colon cancer marker Download PDF

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Publication number
CA2518933A1
CA2518933A1 CA002518933A CA2518933A CA2518933A1 CA 2518933 A1 CA2518933 A1 CA 2518933A1 CA 002518933 A CA002518933 A CA 002518933A CA 2518933 A CA2518933 A CA 2518933A CA 2518933 A1 CA2518933 A1 CA 2518933A1
Authority
CA
Canada
Prior art keywords
biological sample
colon cancer
cdna
tumor marker
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002518933A
Other languages
French (fr)
Other versions
CA2518933C (en
Inventor
Shigeru Kanaoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NATIONAL UNIVERSITY CORPORATION, HAMAMATSU UNIVERSITY SCHOOL OF MEDICI
Olympus Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2518933A1 publication Critical patent/CA2518933A1/en
Application granted granted Critical
Publication of CA2518933C publication Critical patent/CA2518933C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57407—Specifically defined cancers
    • G01N33/57419—Specifically defined cancers of colon

Abstract

It is intended to provide a non-invasive and convenient method of detecting a tumor marker for diagnosing colon cancer which is superior in sensitivity and specificity to the existing fecal occult blood test.
More specifically speaking, a method of detecting a tumor marker for diagnosing colon cancer which comprises collecting biological sample which is immediately frozen using liquid nitrogen in some cases, homogenizing the sample in the presence of an inhibitor of an RNA digesting enzyme to give a suspension, extracting RNA from the obtained suspension, subjecting the extracted RNA to reverse transcription to give cDNA, amplifying the obtained cDNA and then detecting the thus amplified cDNA.
This method is characterized by involving no procedure of separating cell components from the biological sample.

Claims (12)

1. A method for preparing a sample to extract RNA used in a tumor marker detecting method for diagnosing colon cancer, comprising the following process;
a) a process to homogenize the collected biological sample in the presence of an RNase inhibitor, to prepare a suspension thereof;
characterized by involving no procedure of separating cell components from the biological sample.
2. A method according to claim 1, wherein the collected biological sample is frozen.
3. A method according to claim 1 or 2, wherein the RNase inhibitor is guanidine thiocyanate.
4. A method according to any one of claim 1 to 3, wherein the biological sample is feces.
5. A tumor marker detecting method for diagnosing colon cancer, comprising the following processes:
b) a process to extract RNA from the obtained sample for extracting RNA;
c) a process to reverse transcribe the extracted RNA to give cDNA;
d) a process to amplify the obtained cDNA; and e) a process to detect the amplified cDNA, in addition to the method according to any one of claim 1 to 4.
6. A method according to any one of claim 1 to 5, wherein the tumor marker is COX-2.
7. A kit for preparing a sample to extract RNA used in a tumor marker detecting method for diagnosing colon cancer, comprising the following means;
a) a means to homogenize a collected biological sample in the presence of an RNase inhibitor, and prepare a suspension thereof;
characterized by involving no means for separating cell components from the biological sample.
8. A kit according to claim 7, comprising further a means to freeze the collected biological sample.
9. A kit according to claim 7 or claim 8, wherein the RNase inhibitor is guanidine thiocyanate.
10. A kit according to any one of claim 7 to 9, wherein the biological samples are feces.
11. A tumor marker detecting kit for diagnosing colon cancer, further comprising the following means:
b) a means to extract RNA from the obtained sample for extracting RNA;
c) a means to reverse transcribe the extracted RNA to give cDNA.
d) a means to amplify the obtained cDNA; and e) a means to detect the amplified cDNA.
12. A kit according to any one of claim 7 to 11, wherein the tumor marker is COX-2.
CA2518933A 2003-03-19 2003-09-19 Method of detecting colon cancer marker Expired - Fee Related CA2518933C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2003075552 2003-03-19
JP2003-75552 2003-03-19
PCT/JP2003/011972 WO2004083856A1 (en) 2003-03-19 2003-09-19 Method of detecting colon cancer marker

Publications (2)

Publication Number Publication Date
CA2518933A1 true CA2518933A1 (en) 2004-09-30
CA2518933C CA2518933C (en) 2012-04-03

Family

ID=33027864

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2518933A Expired - Fee Related CA2518933C (en) 2003-03-19 2003-09-19 Method of detecting colon cancer marker

Country Status (10)

Country Link
US (3) US7816077B2 (en)
EP (1) EP1605261B1 (en)
JP (2) JP4134047B2 (en)
CN (1) CN100567985C (en)
AT (1) ATE451617T1 (en)
AU (1) AU2003264512A1 (en)
CA (1) CA2518933C (en)
DE (1) DE60330505D1 (en)
ES (1) ES2335344T3 (en)
WO (1) WO2004083856A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4134047B2 (en) * 2003-03-19 2008-08-13 財団法人浜松科学技術研究振興会 Colorectal cancer marker detection method
JP4798514B2 (en) * 2005-08-10 2011-10-19 国立大学法人浜松医科大学 Colorectal cancer marker detection method
JP4477575B2 (en) * 2005-12-14 2010-06-09 株式会社日立製作所 Gene set used for colorectal cancer testing
JPWO2008093530A1 (en) * 2007-01-30 2010-05-20 国立大学法人浜松医科大学 Colorectal cancer stage detection method
JP2009065900A (en) * 2007-09-13 2009-04-02 Olympus Corp Methods for analyzing and preserving target rna
US20100255481A1 (en) * 2007-10-30 2010-10-07 Olympus Corporation Method for detection of adenoma or cancer by genetic analysis
WO2009139317A1 (en) * 2008-05-12 2009-11-19 オリンパス株式会社 Method of processing excrement and container for processing excrement
JP4538525B2 (en) * 2009-01-16 2010-09-08 千絵 宮本 Method for detecting cancer-related gene markers
JP2011250757A (en) * 2010-06-03 2011-12-15 Olympus Corp Method for detecting nucleic acid in biological sample
JPWO2012014694A1 (en) * 2010-07-26 2013-09-12 オリンパス株式会社 Method for synthesizing fecal nucleic acid
GB201107466D0 (en) 2011-05-05 2011-06-15 Loktionov Alexandre Device and method for non-invasive collection of colorectal mucocellular layer and disease detection

Family Cites Families (17)

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Publication number Priority date Publication date Assignee Title
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
NL8900725A (en) * 1989-03-23 1990-10-16 Az Univ Amsterdam METHOD AND COMBINATION OF AGENTS FOR INSULATING NUCLEIC ACID.
US5654179A (en) 1990-11-14 1997-08-05 Hri Research, Inc. Nucleic acid preparation methods
US5777099A (en) 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
EP0938320B2 (en) * 1996-03-26 2014-06-18 Michael S. Kopreski Method enabling use of extracellular rna extracted from plasma or serum to detect, monitor or evaluate cancer
WO1998045480A1 (en) * 1997-04-04 1998-10-15 The Texas A & M University System Noninvasive detection of colonic biomarkers using fecal messenger rna
US6406857B1 (en) * 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
EP0939118A1 (en) * 1998-02-20 1999-09-01 Universiteit Maastricht Method for isolating DNA and RNA from faeces
US6335193B1 (en) 1999-04-15 2002-01-01 Padmanabhan P Nair Isolated colonocytes
JP2002142767A (en) 2000-11-06 2002-05-21 Fujirebio Inc Method for detecting cancer and method for determining whether carcinogenesis advance or not
CA2439402A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
EP2290095A1 (en) 2002-06-25 2011-03-02 InDex Diagnostics AB Method and kit for the diagnosis of ulcerative colitis
JP4134047B2 (en) * 2003-03-19 2008-08-13 財団法人浜松科学技術研究振興会 Colorectal cancer marker detection method
JP3805330B2 (en) 2003-08-20 2006-08-02 独立行政法人科学技術振興機構 Test method for colorectal cancer and colorectal adenoma
DE10345021A1 (en) 2003-09-23 2005-05-04 Univ Potsdam Method for the non-invasive early detection of colon cancer and / or colon cancer precursor cells
JP4798514B2 (en) 2005-08-10 2011-10-19 国立大学法人浜松医科大学 Colorectal cancer marker detection method
US7939255B2 (en) 2006-07-03 2011-05-10 Catholic University Industry Academy Cooperation Foundation Diagnostic methods for colorectal cancer

Also Published As

Publication number Publication date
JP2008271969A (en) 2008-11-13
EP1605261B1 (en) 2009-12-09
AU2003264512A1 (en) 2004-10-11
US20090291447A1 (en) 2009-11-26
JP4206425B2 (en) 2009-01-14
ES2335344T3 (en) 2010-03-25
JP4134047B2 (en) 2008-08-13
JPWO2004083856A1 (en) 2006-06-22
CN1759317A (en) 2006-04-12
US7816077B2 (en) 2010-10-19
WO2004083856A1 (en) 2004-09-30
ATE451617T1 (en) 2009-12-15
US8080378B2 (en) 2011-12-20
DE60330505D1 (en) 2010-01-21
EP1605261A1 (en) 2005-12-14
US20100323367A1 (en) 2010-12-23
EP1605261A4 (en) 2007-08-29
CA2518933C (en) 2012-04-03
US8445202B2 (en) 2013-05-21
CN100567985C (en) 2009-12-09
US20060216714A1 (en) 2006-09-28

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Effective date: 20180919