CN102462561A - Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof - Google Patents
Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof Download PDFInfo
- Publication number
- CN102462561A CN102462561A CN2010105525277A CN201010552527A CN102462561A CN 102462561 A CN102462561 A CN 102462561A CN 2010105525277 A CN2010105525277 A CN 2010105525277A CN 201010552527 A CN201010552527 A CN 201010552527A CN 102462561 A CN102462561 A CN 102462561A
- Authority
- CN
- China
- Prior art keywords
- soft tissue
- solution
- tissue repair
- sis
- sticking patch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention relates to a method for preparing a small intestinal submucosa (SIS) soft tissue repair patch, and the soft tissue repair patch prepared by the method. The invention also relates to application of the soft tissue repair path to tissue repair.
Description
Technical field
The present invention relates to a kind of soft tissue repair sticking patch and preparation method thereof, specifically, the preparation and preparation method thereof that relates to a kind of SIS (small intestinal submucosa, small intestinal submucosa material) tissue renovation material with and uses thereof.
Background technology
Tissue injury or damaged be common clinically disease, like the wound, the organ-tissue segmental defect that cause of operation in the body, hernia, skin surface damage etc., it has a strong impact on patient's quality of life, is the difficult problem that medically must face and need to be resolved hurrily.
The material that is used for tissue repair in the market mainly contains two types: one type is high molecular synthetic materials such as polypropylene, polyester, politef; But because of it all is the good adhesion carrier of antibacterial; Bacterial adhesion can produce after on it and make its biofilm of avoiding host immune defense mechanism and antibiotic effect, thereby is able in the part to become to live for a long time and can causes the chronic infection of wound; Another kind of is biological tissue's derived material; Constitute through the extracellular matrix that the physics and chemistry processing forms by people's allosome or the membranaceous or lamellar structure of mammal with three dimensions frame structure; All withstood test on its histocompatibility, the stability, but clinical effectiveness is undesirable.Reason is that it is shaped to plain film naturally, and surface porosity factor is low, is unfavorable for that cell attaches, propagation, thereby prolongs the tissue repair time or cause the wound chronic infection.Thereby, need a kind of brand-new soft tissue repair sticking patch of being convenient to use to satisfy clinical demand.
It is the natural biological medical material of deriving that people's allosome or mammiferous membranaceous or lamellar structure are handled the extracellular matrix with three dimensions frame structure that forms through specific process; Application and market prospect are extensive, like surgical wound operation, the reparation of urinary system defective tissue, shaping and beauty etc.The mammal small intestinal is mainly formed by 4 layers, is respectively mucous layer, Submucosa, flesh layer, placenta percreta from inside to outside.Small intestinal submucosa is made up of one deck compact connective, and its structure comprises and be netted arrangement collagen fibers (mainly be I type, III type, also have a small amount of VI type and V-type), by α
1, α
2, α
3Peptide chain is formed.α wherein
1Peptide chain can with the special receptors bind of cell surface, the activation signal pathway promotes adhesion, propagation and the differentiation of cell.A large amount of oh groups also can promote cell adhesion, and collagen protein is participated in cell differentiation, and the control cell adhesion is regulated the cell growth, helps to keep cytostromatic RF.Small intestinal submucosa also contains a large amount of Dan Baijutang, glycoprotein and multiple somatomedin etc. simultaneously; Even after physico-chemical process is handled, activity is arranged still.There is not blood vessel not contain cell again; Implant as allotransplant and can not cause tangible immunological rejection; In experimentation, successfully be applied to repair blood vessel, bladder, bone, cartilage, tendon, meniscal cartilage tissue, demonstrated good potential applicability in clinical practice.
Prove that through big quantity research SIS has excellent biological compatibility, various kinds of cell is had the effect that promotes adhesion, propagation, differentiation.As the in-vivo tissue engineering material, SIS can provide temporary transient mechanical holding power, and in bearing object in the surrounding tissue pressure, for this soma provides the regeneration of growing into of enough growing spaces, 8～12 all SIS degrade fully.In addition, SIS has the ability of the tissue regeneration of site specific, and rapidly inducing cell soaks into, and stimulates growing into and breaking up of angiogenesis and host cell, and the regenerating tissues of generation is all similar with basic stitch on 26S Proteasome Structure and Function.Therefore, no matter be experiment or clinical, SIS has the favorable tissue repairing performance.
Organizational project reparation and timbering material should possess following condition: 1. excellent biological compatibility and no antigen are arranged, not can with receptor generation immunological rejection; 2. degradability and degradation rate controllability; 3. keep growth cellular morphology and phenotype above that, and promote the adhesion and the growth of cell, the complete biological tissue of the final formation of induced tissue regeneration; 4. nutrition and the exchange of material air of certain gap rate between being convenient to organize arranged; 5. there is certain mechanical strength to play the support effect to tissue.
SIS almost has above-mentioned all characteristics as a kind of n cell epimatrix biomaterial.At present simple about the preparation process of SIS; Be confined to mostly take off on the cell separately; All can there be the defat deproteinization on the whole or the inadequate problem of sterilizing; Thereby cause material to have certain immunogenicity and antigenicity, its single-layer products mechanical strength of while is low and the shape of product structure is single, is unfavorable for the reparation of soft tissue.
Summary of the invention
One of the object of the invention is preparation a kind of SIS (small intestinal submucosa, small intestinal submucosa material) soft tissue repair sticking patch.
In order to realize the object of the invention, the present invention adopts processing methods such as physical chemistry and biology, the membranaceous or lamellar structure such as people's allosome or mammal is carried out disinfection, takes off cell, a series of processing such as crosslinked, again through mould compression moulding.
SIS soft tissue repair sticking patch of the present invention is to adopt people's allosome or the membranaceous or lamellar structure of mammal, the treated n cell epimatrix class bio-derived material that processes.
Membranaceous or the lamellar structure of mammals such as the material source behaviour of SIS soft tissue repair sticking patch, pig, sheep, Canis familiaris L., cattle or other animal can adopt pig small intestine or sheep small intestine such as raw material.Mainly be to remove fat, placenta percreta, flesh layer and mucous layer in membranaceous or the lamellar structure through Mechanical Method.Obtain Submucosa.
Said processing method to SIS is the combination between processing methods such as Mechanical Method, physical-chemical process and biology.
The method for preparing of SIS soft tissue repair sticking patch of the present invention; It comprises the steps: the small intestinal submucosa material is cleaned, disinfects, ungrease treatment, takes off cell processing, crosslinking Treatment, drilling and handle with random order, after lyophilization form.
Wherein, described disinfecting to adopting disinfectant solution that small intestinal submucosa is carried out immersion treatment at normal temperatures.The mass volume ratio of SIS and disinfectant solution is 1: 1-10 (g/ml).
Concrete disinfection way is: soaked 10-60 minute with the peracetic acid of 0.05-2% and the alcoholic acid mixed aqueous solution of 1-10%; Perhaps the disodium ethylene diamine tetra-acetic acid solution of 0.01-0.05M soaked 1-6 hour; Perhaps the 0.01-1% sodium hydroxide solution soaked 4-12 hour, and the neomycin sulphate solution of 5-20% soaks and carried out the combined disinfection processing in 15-60 minute.
Disinfected the back with the thorough ultrasonic cleaning of purified water, can change purified water when cleaning as required and repeatedly clean, generally cleaned 1-5 time.
Said ungrease treatment was soaked 6-12 hour for adopting under the organic solvent room temperature SIS through 1-5 immersion treatment at every turn.The mass volume ratio of SIS and organic solvent is 1: 1-10 (g/ml).
Said organic solvent is one or more blended solution in dehydrated alcohol, methanol, chloroform, dichloromethane, the chloroform.Preferred mixed proportion is 1: 8-1: 6.Will renew bright solution, for guaranteeing abundant defat, processing procedure can be through the aid in treatment of ultrasonication at every turn; Handle the back with 1-5 ultrasonic cleaning of purified water.
Said to take off that cell handles be to handle with having the solution soaking of taking off cytosis room temperature under, and SIS and the volume ratio of taking off cell solution are 1: 1-10 (g/ml).The said cell solution that takes off is hypertonic saline (sodium chloride solution of 5-10% soaked 12-48 hour); Enzymatic solution (trypsin solution and/or 30-50U/ml DNA enzymatic solution like 0.05-0.5% (g/ml) soaked 12-36 hour); Acid solution (soaking 1-4 hour) like the 0.5-1M hydrochloric acid solution; Aqueous slkali (soaking 2-8 hour) like the 0.01-1% sodium hydroxide solution; One or more mixed solutions in the saline solution (disodium ethylene diamine tetra-acetic acid solution like 0.01-0.05M soaked 1-6 hour) carry out immersion treatment.The above-mentioned cell that takes off is handled and can be reached good biocompatibility.
Take off cell and handle the back, can change purified water when cleaning as required and repeatedly clean, generally clean 1-4 time with the thorough ultrasonic cleaning of purified water.
Said crosslinking Treatment is to adopt cross-linking agent glutaraldehyde (0.2-0.8%, pH=7.2-7.4), genipin (0.5-1.5%, pH=7.2-7.4) to wait one or more mixed solutions to carry out immersion treatment 12-48 hour under the room temperature.
It is to adopt the sodium chloride solution of 1-3M to soak room temperature under 12-24 hour that said drilling is handled, and makes the sample dehydration through lyophilization then, more dried sample is immersed in the pure water 12-48 hour, at last under special mould is suppressed through the lyophilization molding.Obtain the product (referring to Fig. 2) of big surface porosity factor thus, and improve porosity, can help attaching, migration, propagation and the differentiation of normal surrounding tissue cell, thereby be beneficial to wound healing.
Said product through special mould compression moulding handle be with the loose one side in sample surfaces space be serosal surface toward the outer side; The one side of space densification is towards the inboard relatively; Again with serosal surface and the alternately stack of mucosal surface multilamellar; Place the mould of special construction, the adjusting of associating freezer dryer condition is pressed into poroid, horizontal vertical staggered latticed, conical form that cluster is integrated, braid form etc. with product and satisfies the special shape (like Fig. 3 and 4) that tissue repair requires.
Small intestinal submucosa of the present invention (SIS) can be carried out surface sterilizing and disinfect small intestinal submucosa earlier, such as carrying out immersion treatment with bromo geramine and iodine tincture before processing.
In sterilization of the present invention, defat, take off in cell and the crosslinking Treatment process, also can suitably add buffer solution such as phosphate, sodium chloride, sodium carbonate and regulate the solution system balance in the PROCESS FOR TREATMENT.
In the present invention, adopt frozen solution Hanks balanced salt solution (by weight adding 0.1-10% glucose, 0.1-5% sucrose, 0.1-5% trehalose, 0.1-0.5% human serum albumin) to soak 25min, the protection that sample carries out before the lyophilization is handled.
Described freezing dry process is taked the mode of-80 ℃ of quick-freezings, and with sample pre-freeze 1-6 hour, regulates vacuum condition simultaneously, makes sample steadily distillation rapidly, guarantees the smooth property of product surface.
SIS soft tissue repair sticking patch of the present invention can further be processed into the film like material that thickness in monolayer is 10-200um; The relative mucosa one side of small intestinal submucosa serosal surface one side is more coarse, and the space is bigger between tissue, and it is beneficial to cell and attaches growth; So when product multilamellar stack molding, take into full account this advantage; Be that the alternative mode of placenta percreta and mucous layer superposes, guarantee placenta percreta simultaneously, can be divided into monolayer, bilayer, four layers, six layers, eight layers etc. according to the different purposes of product towards the outside.Composite bed forms through cross-linking agent and/or physics pressurization.
The present invention relates to
1. the method for preparing of a SIS soft tissue repair sticking patch is characterized in that, comprises the membranaceous or lamellar structure of animal is processed the extracellular matrix with three dimensions frame structure through taking off the cell processing.
2. according to the method for preparing of item 1 described SIS soft tissue repair sticking patch, it comprises the steps: membranaceous or lamellar structure are cleaned, disinfected, ungrease treatment, take off cell processing, crosslinking Treatment, drilling and handle, and lyophilization.
3. according to the method for preparing of item 1 or 2 described SIS soft tissue repair sticking patch, wherein said animal is membranaceous or lamellar structure is behaved allosome or the membranaceous or lamellar structure of mammal, for example pig small intestine or sheep small intestine.
4. according to the method for preparing of each described SIS soft tissue repair sticking patch among the item 1-3, wherein said membranaceous or lamellar structure is the small intestinal submucosa material.
5. according to the method for preparing of item 4 described SIS soft tissue repair sticking patch, it is characterized in that described disinfecting to adopting disinfectant solution that small intestinal submucosa is carried out immersion treatment at normal temperatures.
6. according to the method for preparing of item 5 described SIS soft tissue repair sticking patch; It is characterized in that; The processing mode of said disinfectant solution is: soaked 10-60 minute with the peracetic acid of 0.05-2% and the alcoholic acid mixed aqueous solution of 1-10%; Perhaps the 0.01-1% sodium hydroxide solution soaked 4-12 hour, and the neomycin sulphate solution of 5-20% soaked 15-60 minute.
7. according to the method for preparing of each described SIS soft tissue repair sticking patch among the item 2-6, it is characterized in that said ungrease treatment was soaked 6-12 hour for adopting under the organic solvent room temperature SIS through 1-5 immersion treatment at every turn.
8. according to the method for preparing of item 7 described SIS soft tissue repair sticking patch; It is characterized in that; Said organic solvent is one or more blended solution that are selected from dehydrated alcohol, methanol, chloroform, dichloromethane and the chloroform; The mass volume ratio of preferred SIS and organic solvent is 1: 1-10 (g/ml), also preferred mixed proportion is 1: 8-1: 6.
9. according to the method for preparing of each described SIS soft tissue repair sticking patch among the item 1-8; It is characterized in that said to take off that cell handles be that one or more mixed solutions that usefulness is selected from hypertonic saline, enzymatic solution, acid solution, aqueous slkali or the saline solution room temperature under carry out immersion treatment.
10. according to the method for preparing of item 9 described SIS soft tissue repair sticking patch, it is characterized in that said hypertonic saline solution-treated is that 10% sodium chloride solution soaked 12-48 hour; The trypsin solution that said enzymatic solution is treated to 0.05-0.5% soaked 12-36 hour; Said acid solution is treated to the 0.5-1M hydrochloric acid solution and soaked 1-4 hour; Said aqueous slkali is treated to the 0.01-1% sodium hydroxide solution and soaked 2-8 hour; The disodium ethylene diamine tetra-acetic acid solution that said saline solution is treated to 0.01-0.05M soaked 1-6 hour.
11. the method for preparing according to each described SIS soft tissue repair sticking patch among the item 2-10 is characterized in that, said crosslinking Treatment is to adopt one or more mixed solutions such as cross-linking agent glutaraldehyde, genipin to carry out immersion treatment under the room temperature.
12. method for preparing according to each described SIS soft tissue repair sticking patch among the item 2-11; It is characterized in that said crosslinking Treatment is to adopt genipin (pH=7.2-7.4) solution soaking of the glutaraldehyde (pH=7.2-7.4) of 0.2-0.8%, 0.5-1.5% to handle under the room temperature 12-48 hour.
13. method for preparing according to each described SIS soft tissue repair sticking patch among the item 2-12; It is characterized in that; Described crosslinking Treatment is to adopt placenta percreta and the alternative mode of mucous layer to superpose single-layer products, when product multilamellar stack molding, guarantees that placenta percreta is towards the outside.
14. according to the method for preparing of each described SIS soft tissue repair sticking patch among the item 2-13, it is characterized in that according to the different purposes of product with its be made as single or multiple lift such as double-deck, four layers, six layers, eight layers.
15. method for preparing according to each described SIS soft tissue repair sticking patch among the item 2-14; It is characterized in that; It is to adopt the sodium chloride solution of 1-3M to soak under the room temperature 12-24 hour that said drilling is handled; Make sample dehydration through lyophilization then, more dried sample be immersed in the pure water 12-48 hour, at last under special mould compacting through the lyophilization molding.
16. method for preparing according to each described SIS soft tissue repair sticking patch among the item 2-15; It is the mould that sample is placed special construction that said product is handled through special mould compression moulding; The adjusting of associating freezer dryer condition; Product is pressed into satisfies the special shape that tissue repair requires, such as poroid, horizontal vertical staggered latticed, conical form, braid form that cluster is integrated.
17. soft tissue repair sticking patch according to each method preparation among the item 1-16.
18. 17 described soft tissue repair sticking patch, after it is characterized in that handling through special perforating agent, product surface forms abundant mesh-structured.
19. the purposes of soft tissue repair sticking patch in tissue repair described in the item 17 or 18, such as, tissue injury or damaged, for example reparation, the reinforcement of damage or weak soft tissue repaired like hernia repair, urinary system tissue defect; Repair soft tissue defects in the body body surface body, as organizing filler, serving as that to organize stiffener to substitute fascia, repair membrane property damaged etc.
SIS soft tissue repair sticking patch of the present invention, the extracellular matrix with three dimensions frame structure, the reparation that is used to organize.The SIS soft tissue repair sticking patch of the present invention's preparation, it contains abundant fibroblast and various kinds of cell somatomedin and extracellular matrix components, like collagen protein, elastin laminin, glycosaminoglycan, Dan Baijutang etc.It is damaged directly to be used to repair soft tissue organs, has three-dimensional netted loose structure, and product of the present invention has big surface porosity factor.SIS soft tissue repair sticking patch of the present invention is a heterogenous cell epimatrix of having removed cell and native antigen composition, has than high bioactivity and biocompatibility non-immunogenicity and cytotoxicity.
SIS soft tissue repair sticking patch of the present invention; Its surface has multiple shape after special mould compression moulding; Be suitable for difform wound site of body and tissue defect district; Satisfy the requirement of strength that different tissues is repaired simultaneously, coarse relatively product surface also helps adhesion, propagation and the differentiation of normal surrounding tissue cell, strengthens the soft tissue repair effect.
The preparation of SIS soft tissue repair sticking patch of the present invention, its technical process strictness is complete, has stronger systematicness and integrity; Can when guaranteeing properties of product, reach effective removal product immunogenicity, the repulsion and the anaphylaxis that produce when reducing tissue repair; Reduce less patient suffering and operation risk; Improve safety, and need not combine with other film, the SIS soft tissue repair sticking patch of the inventive method preparation itself just can be avoided immunological rejection; Moreover the residual quantity of cross-linking agent can also reach within the scope of permission, when strengthening the product mechanical strength, improved safety.
SIS soft tissue repair sticking patch can be used for damaging or reparation, the reinforcement of weak soft tissue clinically, repairs like hernia repair, urinary system tissue defect; Repair soft tissue defects in the body body surface body, as organizing filler, serving as that to organize stiffener to substitute fascia, repair membrane property damaged etc.
Description of drawings
Metamorphosis and peripheral tissues's growing state of Fig. 1 SIS soft tissue repair sticking patch.A:SIS implanted 4 days, and peripheral tissues and SIS sticking patch organizational structure are more clear, and cell number increases, and inflammatory cell is arranged; B:SIS implanted 12 days, and peripheral tissues and SIS sticking patch organizational structure are inseparable, and cell number is more, rare inflammatory cell.
The comparison of the scanning electron microscope picture surface void structure on Fig. 2 SIS sticking patch of the present invention (A) and the market under the equal conditions of the product (B) of COOK company.
Fig. 3 SIS soft tissue repair sticking patch.
Fig. 4 SIS soft tissue repair sticking patch prepares the mould picture that molding is adopted.
The specific embodiment
Following examples are used to explain the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Get the fresh pig small intestinal of butchering in 6 hours, pig jejunum (between duodenum and the ileum) is clean with distilled water flushing, be cut into the 20cm fragment; Be immersed in 30min in 0.5% the bromo geramine solution; Longitudinally cut open after the rinsing of reuse pure water, mechanical curettage mucous membrane of small intestine, flesh layer and placenta percreta place the alcoholic acid mixed aqueous solution soaking disinfection 60 minutes that contains 0.2% peracetic acid and 5%; Use the normal saline rinsing, obtain small intestinal submucosa; Placed disodiumedetate (0.05M) solution again 2 hours and the solution of sodium hydroxide (1%) (ph value for 11-12) soaked 8 hours, temperature is 4 ℃ ± 2 ℃, and small intestinal submucosa is carried out preliminarily pasteurized and taken off the cell processing; Again it is immersed in 4h in hydrochloric (1mol/L) solution, places phosphate buffer (PBS) rinsing to neutral; Adopt dehydrated alcohol that it was carried out ungrease treatment 12 hours again, the volume ratio of sample and solvent is 1: 4 (g/ml); Being placed in 10% sodium chloride solution soak at room temperature after the clear water rinsing again 24 hours, and containing the DNA enzyme with 0.5% the trypsin that contains 0.25% ethylenediaminetetraacetic acid afterwards) solution soaks for 37 ℃ and took off cell in 24 hours and handle, is placed on again in the sodium chloride solution of 3mol/L to continue to stir 24 hours; Take serosal surface afterwards toward the outer side, mucosal surface is towards the method for internal layer, and each layer be stack alternately; Be tiled in and have on the latticed 316L corrosion resistant plate, carry out lyophilization, adopt 0.625% cross-linking agent glutaraldehyde (pH=7.4) room temperature that small intestinal submucosa is carried out crosslinking Treatment again; Time is 24 hours; Make between each layer of sample crosslinked action takes place, strengthen its mechanical strength, adopt frozen solution to soak sample 6 hours again; Carry out the lyophilization (80 ℃ of pre-freezes 4 hours ,-55 ℃ lyophilizing 48 hours) of last molding afterwards.
Embodiment 2
The pentobarbital sodium anesthetis intraperitoneal injection of anesthesia mice of employing 3%; The subcutaneous Implantation Test of being correlated with again, method: earlier the mouse back hair is cleaned out, it is subcutaneous to adopt card punch respectively product of the present invention (embodiment 1) to be implanted mice back both sides again; Carry out skin suture again, and carry out disinfection.Postoperative animal fasting 12h, sub-cage rearing, continuous three days intramuscular injection penicillins.Observe the performance of mice general state and cutting part.Put to death mice at 4,12,21 days simultaneously, observe metamorphosis and peripheral tissues's growing state of SIS soft tissue repair sticking patch.
The result: postoperative mice growing state is good, and surgical wound healed basically in 4 days, and the pathological section result shows that SIS soft tissue repair sticking patch has better biocompatibility, and the inducing peripheral normal tissue cell attaches, grows, breeds simultaneously.Show better tissues repairing effect (referring to accompanying drawing 1).
The SIS soft tissue repair sticking patch of the present invention's preparation can be preserved 12 months after taking off cell processing, multilamellar crosslinking Treatment, lyophilization; Be convenient to clinical use; It contains abundant fibroblast and various kinds of cell somatomedin and extracellular matrix components, like collagen protein, elastin laminin, glycosaminoglycan, Dan Baijutang etc.It is damaged directly to be used to repair soft tissue organs; The SIS soft tissue repair sticking patch of this examples preparation has certain mechanical strength and abundant three-dimensional netted loose structure simultaneously, can promote the normal surrounding tissue cell to wound site migration, filling, propagation and differentiation.Can effectively promote wound healing, suppress cicatrization and wound surface and reinvent that product of the present invention has big surface porosity factor (Fig. 2).SIS soft tissue repair sticking patch of the present invention is a heterogenous cell epimatrix of having removed cell and native antigen composition, has than high bioactivity and biocompatibility non-immunogenicity and cytotoxicity.SIS soft tissue repair sticking patch can be used for damaging or reparation, the reinforcement of weak soft tissue clinically, repairs like hernia repair, urinary system tissue defect; Repair soft tissue defects in the body body surface body, as organizing filler, serving as that to organize stiffener to substitute fascia, repair membrane property damaged etc.
It is understandable that top description only is an example of the present invention, thereby the scope that claim of the present invention is protected limits with particular disclosed herein not merely.Any equivalent embodiments will be deemed to be within the scope of the present invention.In fact, according to the description of front, the present invention is carried out relevant modification and changes all will be possible to those skilled in the art, thereby this modification and variation also will drop within the scope of the appended claim of the present invention.
Claims (10)
1. the method for preparing of a SIS soft tissue repair sticking patch is characterized in that, comprises the membranaceous or lamellar structure of animal is processed the extracellular matrix with three dimensions frame structure through taking off the cell processing.
2. the method for preparing of SIS soft tissue repair sticking patch according to claim 1, it comprises the steps: membranaceous or lamellar structure are cleaned, disinfected, ungrease treatment, take off cell processing, crosslinking Treatment, drilling and handle, and lyophilization; Preferred said animal is membranaceous or lamellar structure is behaved allosome or the membranaceous or lamellar structure of mammal, for example pig small intestine or sheep small intestine; Preferred wherein said membranaceous or lamellar structure is the small intestinal submucosa material.
3. the method for preparing of SIS soft tissue repair sticking patch according to claim 2 is characterized in that, described disinfecting to adopting disinfectant solution that small intestinal submucosa is carried out immersion treatment at normal temperatures; The processing mode of preferred said disinfectant solution is: soaked 10-60 minute with the peracetic acid of 0.05-2% and the alcoholic acid mixed aqueous solution of 1-10%; Perhaps the 0.01-1% sodium hydroxide solution soaked 4-12 hour, and the neomycin sulphate solution of 5-20% soaked 15-60 minute.
4. according to the method for preparing of each described SIS soft tissue repair sticking patch among the claim 2-3, it is characterized in that said ungrease treatment was soaked 6-12 hour for adopting under the organic solvent room temperature SIS through 1-5 immersion treatment at every turn; Preferred said organic solvent is one or more blended solution that are selected from dehydrated alcohol, methanol, chloroform, dichloromethane and the chloroform; The mass volume ratio of preferred SIS and organic solvent is 1: 1-10 (g/ml), also preferred mixed proportion is 1: 8-1: 6.
5. according to the method for preparing of each described SIS soft tissue repair sticking patch among the claim 2-4; It is characterized in that said to take off that cell handles be that one or more mixed solutions that usefulness is selected from hypertonic saline, enzymatic solution, acid solution, aqueous slkali or the saline solution room temperature under carry out immersion treatment; Preferred said hypertonic saline solution-treated is that 10% sodium chloride solution soaked 12-48 hour; The trypsin solution that said enzymatic solution is treated to 0.05-0.5% soaked 12-36 hour; Said acid solution is treated to the 0.5-1M hydrochloric acid solution and soaked 1-4 hour; Said aqueous slkali is treated to the 0.01-1% sodium hydroxide solution and soaked 2-8 hour; The disodium ethylene diamine tetra-acetic acid solution that said saline solution is treated to 0.01-0.05M soaked 1-6 hour.
6. according to the method for preparing of each described SIS soft tissue repair sticking patch among the claim 2-5, it is characterized in that said crosslinking Treatment is to adopt one or more mixed solutions such as cross-linking agent glutaraldehyde, genipin to carry out immersion treatment under the room temperature; Preferred said crosslinking Treatment is to adopt genipin (pH=7.2-7.4) solution soaking of the glutaraldehyde (pH=7.2-7.4) of 0.2-0.8%, 0.5-1.5% to handle under the room temperature 12-48 hour; Also preferred described crosslinking Treatment is to adopt placenta percreta and the alternative mode of mucous layer to superpose single-layer products, when product multilamellar stack molding, guarantees that placenta percreta is towards the outside; Also preferably be according to the different purposes of product with its be made as single or multiple lift such as double-deck, four layers, six layers, eight layers.
7. according to the method for preparing of each described SIS soft tissue repair sticking patch among the claim 2-6; It is characterized in that; It is to adopt the sodium chloride solution of 1-3M to soak under the room temperature 12-24 hour that said drilling is handled; Make sample dehydration through lyophilization then, more dried sample be immersed in the pure water 12-48 hour, at last under the mould compacting through the lyophilization molding; It is the mould that sample is placed special construction that also preferred said product is handled through mould compression moulding; The adjusting of associating freezer dryer condition; Product is pressed into satisfies the special shape that tissue repair requires, such as poroid, horizontal vertical staggered latticed, conical form, braid form that cluster is integrated.
8. the soft tissue repair sticking patch for preparing according to each method among the claim 1-7.
9. the described soft tissue repair sticking patch of claim 8, after it is characterized in that handling through special perforating agent, product surface forms abundant mesh-structured.
10. the purposes of soft tissue repair sticking patch in tissue repair described in the claim 8 or 9, such as, tissue injury or damaged, for example reparation, the reinforcement of damage or weak soft tissue repaired like hernia repair, urinary system tissue defect; Repair soft tissue defects in the body body surface body, as organizing filler, serving as that to organize stiffener to substitute fascia, repair membrane property damaged etc.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105525277A CN102462561A (en) | 2010-11-19 | 2010-11-19 | Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105525277A CN102462561A (en) | 2010-11-19 | 2010-11-19 | Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102462561A true CN102462561A (en) | 2012-05-23 |
Family
ID=46066877
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010105525277A Pending CN102462561A (en) | 2010-11-19 | 2010-11-19 | Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102462561A (en) |
Cited By (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191466A (en) * | 2013-04-15 | 2013-07-10 | 潘华倩 | Method for preparing human body or animal accellular tissues |
| CN103251987A (en) * | 2013-04-07 | 2013-08-21 | 陕西佰傲再生医学有限公司 | Acellular biological patch, preparation method and apparatus thereof |
| CN103272275A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Biological repair tablet for endocranium and preparation method thereof |
| CN103272277A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Mold for preparing accellular small intestine submucosa matrix material |
| CN103272273A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Mold for preparing accellular small intestine submucosa matrix material |
| CN103272274A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Biological repair tablet for herniae and preparation method thereof |
| CN103301508A (en) * | 2013-05-28 | 2013-09-18 | 北京博辉瑞进生物科技有限公司 | Preparation method of medical cartilage support material |
| CN103301507A (en) * | 2013-05-28 | 2013-09-18 | 北京博辉瑞进生物科技有限公司 | Artificial biological tendon and preparation method thereof |
| CN103751844A (en) * | 2014-01-08 | 2014-04-30 | 重庆市畜牧科学院 | Preparation method and application of antimicrobial and anti-degradable small instestinal mucous accellular substrate |
| CN104107458A (en) * | 2013-04-17 | 2014-10-22 | 四川大学华西医院 | Digestive tract mucous membrane restoration promotion material, and preparation method and use thereof |
| CN105126171A (en) * | 2015-08-27 | 2015-12-09 | 中国人民解放军第三军医大学 | Gel biological material having shape memory function and preparation method of gel biological material |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
| CN105664258A (en) * | 2016-02-22 | 2016-06-15 | 江苏期佰医疗技术有限公司 | Method for preparing smooth muscle cell carrier based on small intestine submucosa acellular matrix |
| CN105727366A (en) * | 2016-02-22 | 2016-07-06 | 江苏期佰医疗技术有限公司 | Preparation method of SIS tissue repair material and application of preparation method |
| CN105749357A (en) * | 2016-04-29 | 2016-07-13 | 陕西瑞盛生物科技有限公司 | Breast patch and preparation method thereof |
| JP2016521592A (en) * | 2013-05-28 | 2016-07-25 | 北京博▲輝▼瑞▲進▼生物科技有限公司Ｂｅｉｊｉｎｇ Ｂｉｏｓｉｓ Ｈｅａｌｉｎｇ Ｂｉｏｌｏｇｉｃａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏ．， Ｌｔｄ． | Method for preparing animal-derived implantable medical biomaterial |
| CN105963782A (en) * | 2016-04-28 | 2016-09-28 | 陕西瑞盛生物科技有限公司 | Biological membrane and preparation method thereof |
| CN105963783A (en) * | 2016-04-29 | 2016-09-28 | 陕西瑞盛生物科技有限公司 | Cross-linking method of biological patch and cross-linked biological patch |
| CN106420109A (en) * | 2016-11-14 | 2017-02-22 | 陕西佰傲再生医学有限公司 | Composite porous cross-linked breast patch and preparation method |
| CN106822999A (en) * | 2017-03-03 | 2017-06-13 | 北京博辉瑞进生物科技有限公司 | A kind of breast patch and its methods for making and using same |
| CN106880872A (en) * | 2016-12-23 | 2017-06-23 | 北京大清生物技术股份有限公司 | Natural extracellular matrix biomembrane and preparation method and application |
| CN106983909A (en) * | 2017-03-03 | 2017-07-28 | 北京博辉瑞进生物科技有限公司 | A kind of otology repair materials, preparation method and application |
| CN107007882A (en) * | 2017-03-03 | 2017-08-04 | 北京博辉瑞进生物科技有限公司 | A kind of nerve repair material, preparation method and application |
| CN107213517A (en) * | 2017-05-17 | 2017-09-29 | 宁波大学 | A kind of composite bone cement and preparation method thereof |
| CN107233622A (en) * | 2017-06-20 | 2017-10-10 | 爱美客技术发展股份有限公司 | A kind of preparation method of de- cell tissue film |
| CN107233630A (en) * | 2017-07-06 | 2017-10-10 | 苏州期佰生物技术有限公司 | A kind of complex biological sticking patch based on trees-Osima jacoti, Osima excavata and preparation method and application |
| CN107308496A (en) * | 2017-06-09 | 2017-11-03 | 广州悦清再生医学科技有限公司 | A kind of biological tissue reienforcement timbering material and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN108079363A (en) * | 2017-12-19 | 2018-05-29 | 广州昕生医学材料有限公司 | A kind of kit and its application that cell processing is taken off for animal tissue |
| CN108114320A (en) * | 2018-03-01 | 2018-06-05 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN108126241A (en) * | 2018-03-01 | 2018-06-08 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN108478870A (en) * | 2017-07-12 | 2018-09-04 | 上海白衣缘生物工程有限公司 | A kind of dura mater biological sticking patch and preparation method thereof |
| CN109248339A (en) * | 2018-11-28 | 2019-01-22 | 上海白衣缘生物工程有限公司 | A kind of hernia Biological Repair mesh sheet and preparation method thereof |
| CN109248341A (en) * | 2018-11-20 | 2019-01-22 | 北京航空航天大学 | A kind of soft tissue repair sticking patch and preparation method thereof |
| CN109453428A (en) * | 2018-11-28 | 2019-03-12 | 上海白衣缘生物工程有限公司 | A kind of rotator cuff Biological Repair mesh sheet and application thereof and preparation method |
| CN109498841A (en) * | 2018-11-28 | 2019-03-22 | 冠昊生物科技股份有限公司 | A kind of bion periosteum repair materials and preparation method thereof |
| CN110624132A (en) * | 2019-09-20 | 2019-12-31 | 四川大学华西医院 | Bladder repair stent material and preparation method and application thereof |
| CN111603609A (en) * | 2020-05-25 | 2020-09-01 | 医工瑞思(福建)工程研究中心有限公司 | Bionic tissue engineering scaffold and preparation method thereof |
| CN113717273A (en) * | 2021-09-06 | 2021-11-30 | 北京博辉瑞进生物科技有限公司 | Natural collagen material, preparation method and application thereof |
| CN114344567A (en) * | 2021-12-01 | 2022-04-15 | 宁波大学医学院附属医院 | Achilles tendon repairing patch and preparation method thereof |
| CN114533962A (en) * | 2022-01-10 | 2022-05-27 | 宁波大学医学院附属医院 | Columnar achilles tendon repairing material and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5733337A (en) * | 1995-04-07 | 1998-03-31 | Organogenesis, Inc. | Tissue repair fabric |
| CN1267201A (en) * | 1997-05-08 | 2000-09-20 | 奥加诺吉尼西斯公司 | Chemical cleaning of biological material |
| CN1775189A (en) * | 2005-11-30 | 2006-05-24 | 烟台正海生物技术有限公司 | Acellular dermal matrix and its preparing method |
| CN101172166A (en) * | 2007-11-16 | 2008-05-07 | 广东冠昊生物科技有限公司 | Ocular surface biomembrane and preparation method thereof |
| CN101496912A (en) * | 2008-01-30 | 2009-08-05 | 北京大清生物技术有限公司 | Bio-derivative tendon repair material and preparation method thereof |
| CN101590292A (en) * | 2009-06-11 | 2009-12-02 | 山东省眼科研究所 | The preparation method of acellular conjunctiva matrix and application |
-
2010
- 2010-11-19 CN CN2010105525277A patent/CN102462561A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5733337A (en) * | 1995-04-07 | 1998-03-31 | Organogenesis, Inc. | Tissue repair fabric |
| CN1267201A (en) * | 1997-05-08 | 2000-09-20 | 奥加诺吉尼西斯公司 | Chemical cleaning of biological material |
| CN1775189A (en) * | 2005-11-30 | 2006-05-24 | 烟台正海生物技术有限公司 | Acellular dermal matrix and its preparing method |
| CN101172166A (en) * | 2007-11-16 | 2008-05-07 | 广东冠昊生物科技有限公司 | Ocular surface biomembrane and preparation method thereof |
| CN101496912A (en) * | 2008-01-30 | 2009-08-05 | 北京大清生物技术有限公司 | Bio-derivative tendon repair material and preparation method thereof |
| CN101590292A (en) * | 2009-06-11 | 2009-12-02 | 山东省眼科研究所 | The preparation method of acellular conjunctiva matrix and application |
Cited By (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103251987A (en) * | 2013-04-07 | 2013-08-21 | 陕西佰傲再生医学有限公司 | Acellular biological patch, preparation method and apparatus thereof |
| CN103251987B (en) * | 2013-04-07 | 2014-12-31 | 陕西佰傲再生医学有限公司 | Acellular biological patch, preparation method and apparatus thereof |
| CN103191466A (en) * | 2013-04-15 | 2013-07-10 | 潘华倩 | Method for preparing human body or animal accellular tissues |
| CN105056306B (en) * | 2013-04-17 | 2020-04-28 | 拓睿美（北京）医疗科技有限公司 | Application of material prepared by genipin cross-linking intestinal submucosa |
| CN105056306A (en) * | 2013-04-17 | 2015-11-18 | 四川大学华西医院 | Use of material prepared through cross-linking genipin to intestinal mucosal lower layer |
| CN104107458A (en) * | 2013-04-17 | 2014-10-22 | 四川大学华西医院 | Digestive tract mucous membrane restoration promotion material, and preparation method and use thereof |
| CN103301507A (en) * | 2013-05-28 | 2013-09-18 | 北京博辉瑞进生物科技有限公司 | Artificial biological tendon and preparation method thereof |
| JP2016521592A (en) * | 2013-05-28 | 2016-07-25 | 北京博▲輝▼瑞▲進▼生物科技有限公司Ｂｅｉｊｉｎｇ Ｂｉｏｓｉｓ Ｈｅａｌｉｎｇ Ｂｉｏｌｏｇｉｃａｌ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏ．， Ｌｔｄ． | Method for preparing animal-derived implantable medical biomaterial |
| CN103272274A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Biological repair tablet for herniae and preparation method thereof |
| CN103272273A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Mold for preparing accellular small intestine submucosa matrix material |
| CN103272273B (en) * | 2013-05-28 | 2015-04-08 | 北京博辉瑞进生物科技有限公司 | Mold for preparing accellular small intestine submucosa matrix material |
| CN103301507B (en) * | 2013-05-28 | 2015-04-29 | 北京博辉瑞进生物科技有限公司 | Artificial biological tendon and preparation method thereof |
| CN103301508B (en) * | 2013-05-28 | 2015-05-13 | 北京博辉瑞进生物科技有限公司 | Preparation method of medical cartilage support material |
| CN103272277A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Mold for preparing accellular small intestine submucosa matrix material |
| CN103301508A (en) * | 2013-05-28 | 2013-09-18 | 北京博辉瑞进生物科技有限公司 | Preparation method of medical cartilage support material |
| CN103272275A (en) * | 2013-05-28 | 2013-09-04 | 北京博辉瑞进生物科技有限公司 | Biological repair tablet for endocranium and preparation method thereof |
| CN103751844A (en) * | 2014-01-08 | 2014-04-30 | 重庆市畜牧科学院 | Preparation method and application of antimicrobial and anti-degradable small instestinal mucous accellular substrate |
| CN103751844B (en) * | 2014-01-08 | 2016-02-10 | 重庆市畜牧科学院 | A kind of antibacterial degradation-resistant pig intestinal mucosa acellular matrix preparation method and application thereof |
| CN105126171A (en) * | 2015-08-27 | 2015-12-09 | 中国人民解放军第三军医大学 | Gel biological material having shape memory function and preparation method of gel biological material |
| CN105412986A (en) * | 2015-12-14 | 2016-03-23 | 宁波大学 | SIS (small intestinal submucosa) medicine carrying patch as well as preparation method and application thereof |
| CN105727366A (en) * | 2016-02-22 | 2016-07-06 | 江苏期佰医疗技术有限公司 | Preparation method of SIS tissue repair material and application of preparation method |
| CN105664258A (en) * | 2016-02-22 | 2016-06-15 | 江苏期佰医疗技术有限公司 | Method for preparing smooth muscle cell carrier based on small intestine submucosa acellular matrix |
| CN105963782A (en) * | 2016-04-28 | 2016-09-28 | 陕西瑞盛生物科技有限公司 | Biological membrane and preparation method thereof |
| CN105749357A (en) * | 2016-04-29 | 2016-07-13 | 陕西瑞盛生物科技有限公司 | Breast patch and preparation method thereof |
| CN105963783A (en) * | 2016-04-29 | 2016-09-28 | 陕西瑞盛生物科技有限公司 | Cross-linking method of biological patch and cross-linked biological patch |
| CN106420109A (en) * | 2016-11-14 | 2017-02-22 | 陕西佰傲再生医学有限公司 | Composite porous cross-linked breast patch and preparation method |
| CN106880872A (en) * | 2016-12-23 | 2017-06-23 | 北京大清生物技术股份有限公司 | Natural extracellular matrix biomembrane and preparation method and application |
| CN106880872B (en) * | 2016-12-23 | 2019-10-29 | 北京大清生物技术股份有限公司 | Natural extracellular matrix biomembrane and the preparation method and application thereof |
| CN107007882A (en) * | 2017-03-03 | 2017-08-04 | 北京博辉瑞进生物科技有限公司 | A kind of nerve repair material, preparation method and application |
| CN107007882B (en) * | 2017-03-03 | 2020-01-21 | 北京博辉瑞进生物科技有限公司 | Nerve repair material, preparation method and application |
| CN106822999A (en) * | 2017-03-03 | 2017-06-13 | 北京博辉瑞进生物科技有限公司 | A kind of breast patch and its methods for making and using same |
| CN106983909A (en) * | 2017-03-03 | 2017-07-28 | 北京博辉瑞进生物科技有限公司 | A kind of otology repair materials, preparation method and application |
| CN107213517A (en) * | 2017-05-17 | 2017-09-29 | 宁波大学 | A kind of composite bone cement and preparation method thereof |
| CN107308496A (en) * | 2017-06-09 | 2017-11-03 | 广州悦清再生医学科技有限公司 | A kind of biological tissue reienforcement timbering material and preparation method thereof |
| CN107308496B (en) * | 2017-06-09 | 2020-08-04 | 广州悦清再生医学科技有限公司 | Biological tissue reinforcing scaffold material and preparation method thereof |
| CN107320774A (en) * | 2017-06-16 | 2017-11-07 | 卓阮医疗科技（苏州）有限公司 | One kind splicing biomaterial and preparation method thereof |
| CN107320774B (en) * | 2017-06-16 | 2020-04-07 | 卓阮医疗科技（苏州）有限公司 | Spliced biological material and preparation method thereof |
| CN107233622B (en) * | 2017-06-20 | 2020-05-15 | 爱美客技术发展股份有限公司 | Preparation method of acellular tissue membrane |
| CN107233622A (en) * | 2017-06-20 | 2017-10-10 | 爱美客技术发展股份有限公司 | A kind of preparation method of de- cell tissue film |
| CN107233630A (en) * | 2017-07-06 | 2017-10-10 | 苏州期佰生物技术有限公司 | A kind of complex biological sticking patch based on trees-Osima jacoti, Osima excavata and preparation method and application |
| CN108478870A (en) * | 2017-07-12 | 2018-09-04 | 上海白衣缘生物工程有限公司 | A kind of dura mater biological sticking patch and preparation method thereof |
| CN108079363A (en) * | 2017-12-19 | 2018-05-29 | 广州昕生医学材料有限公司 | A kind of kit and its application that cell processing is taken off for animal tissue |
| CN108114320A (en) * | 2018-03-01 | 2018-06-05 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN108126241A (en) * | 2018-03-01 | 2018-06-08 | 北京迈迪顶峰医疗科技有限公司 | Tissue repair sticking patch, main body and preparation method |
| CN109248341A (en) * | 2018-11-20 | 2019-01-22 | 北京航空航天大学 | A kind of soft tissue repair sticking patch and preparation method thereof |
| CN109453428A (en) * | 2018-11-28 | 2019-03-12 | 上海白衣缘生物工程有限公司 | A kind of rotator cuff Biological Repair mesh sheet and application thereof and preparation method |
| CN109621011A (en) * | 2018-11-28 | 2019-04-16 | 上海白衣缘生物工程有限公司 | A kind of tendon Biological Repair mesh sheet and application thereof and preparation method |
| CN109498841A (en) * | 2018-11-28 | 2019-03-22 | 冠昊生物科技股份有限公司 | A kind of bion periosteum repair materials and preparation method thereof |
| CN109248339A (en) * | 2018-11-28 | 2019-01-22 | 上海白衣缘生物工程有限公司 | A kind of hernia Biological Repair mesh sheet and preparation method thereof |
| CN109498841B (en) * | 2018-11-28 | 2021-07-16 | 冠昊生物科技股份有限公司 | Biological periosteum repair material and preparation method thereof |
| CN110624132A (en) * | 2019-09-20 | 2019-12-31 | 四川大学华西医院 | Bladder repair stent material and preparation method and application thereof |
| CN111603609A (en) * | 2020-05-25 | 2020-09-01 | 医工瑞思(福建)工程研究中心有限公司 | Bionic tissue engineering scaffold and preparation method thereof |
| CN111603609B (en) * | 2020-05-25 | 2022-05-03 | 医工瑞思(福建)工程研究中心有限公司 | Bionic tissue engineering scaffold and preparation method thereof |
| CN113717273A (en) * | 2021-09-06 | 2021-11-30 | 北京博辉瑞进生物科技有限公司 | Natural collagen material, preparation method and application thereof |
| CN114344567A (en) * | 2021-12-01 | 2022-04-15 | 宁波大学医学院附属医院 | Achilles tendon repairing patch and preparation method thereof |
| CN114533962A (en) * | 2022-01-10 | 2022-05-27 | 宁波大学医学院附属医院 | Columnar achilles tendon repairing material and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102462561A (en) | Small intestinal submucosa (SIS) soft tissue repair patch and preparation method thereof | |
| CN202283366U (en) | Small intestinal submucosa (SIS) soft tissue recovery patch | |
| CA2860850C (en) | Sheet tissue products | |
| CN103251987B (en) | Acellular biological patch, preparation method and apparatus thereof | |
| CN104254351B (en) | For ligament or the method for tendon repair | |
| CA2525405C (en) | Compositions for repairing and regenerating human dura mater | |
| US20160199540A1 (en) | Method for preparing an animal decellularized tissue matrix material and a decellularized tissue matrix material prepared thereby | |
| US20030225355A1 (en) | Composite material for wound repair | |
| US20090317469A1 (en) | Composite extracellular matrix materials and medical products formed therefrom | |
| WO2000016822A1 (en) | Compositions and methods for tissue repair | |
| CN102188747A (en) | Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof | |
| CN101366979B (en) | Tissue patch and preparation method thereof | |
| AU2003260086A1 (en) | Composite material for wound repair | |
| CN102143765B (en) | Acellular matrix glue | |
| CN102170920A (en) | Method of manufacturing acellular matrix glue | |
| CN105246495A (en) | Decellularized biomaterial form non-mammalian tissue | |
| US20190015457A1 (en) | Elastic tissue matrix derived hydrogel | |
| CN106913907B (en) | Preparation method of cell growth scaffold with structural memory characteristic | |
| CN104771787A (en) | Composite support containing PGA strengthening net, preparation method and applications thereof | |
| CN112618799A (en) | Fish skin acellular dermal matrix and preparation method and application thereof | |
| CN108114320A (en) | Tissue repair sticking patch, main body and preparation method | |
| CN114533959B (en) | Tendon repair material, preparation method and application in preparation of tendon repair product | |
| CN115845141A (en) | Preparation method and application of dry amnion | |
| CN114288473A (en) | Preparation method of acellular small intestine submucosa composite bone scaffold with antibacterial function | |
| CN108126241A (en) | Tissue repair sticking patch, main body and preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120523 |