CN102520182A - Visible blood glucose test strip and preparation method thereof - Google Patents
Visible blood glucose test strip and preparation method thereof Download PDFInfo
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- CN102520182A CN102520182A CN2011104426974A CN201110442697A CN102520182A CN 102520182 A CN102520182 A CN 102520182A CN 2011104426974 A CN2011104426974 A CN 2011104426974A CN 201110442697 A CN201110442697 A CN 201110442697A CN 102520182 A CN102520182 A CN 102520182A
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Abstract
The invention discloses a visible blood glucose test strip and a preparation method thereof. The visible blood glucose test strip is structurally formed by compounding saturating paper base materials and Millipore nano filter membrane with the bottom face being coated with adhesive. The preparation method comprises the following steps: preparing impregnant solution, preparing the saturating paper base materials, and compounding the saturating paper base materials and the Millipore nano filter membrane. When the test strip is used for blood glucose detection, blood is filtered by the membrane, color development reaction of chromogen reagent, assistant color development reagent and H2O2 is utilized, a special electrochemical signal detection device has no need to be used, so that the effect that blood glucose concentration is quickly and conveniently detected can be achieved. Compared with the test strip with polypropylene, polyethylene, polrvinyl chloride and polycarbonate base materials in the prior art, plant fiber paper base materials of the test strip disclosed by the invention are renewable natural resources, and have the advantages of favorable biodegradation and low price, and the test strip disclosed by the invention can be stored for at least three months at room temperature.
Description
Technical field
The invention belongs to the speciality paper product technical field, relate to a kind of blood sugar test paper and preparation method thereof, relate in particular to a kind of visual blood sugar test paper and preparation method thereof.
Background technology
Diabetes are a kind of common metabolic secretion diseases, are one group of clinical syndromes that is caused by the h and E factor interaction.Because insulin secretion is absolute or relative deficiency, and the target tissue cell reduces insulin sensitivity, causes a series of metabolic disorders such as sugar, protein, fat, power and water Xie Zhi.Diabetes can be divided into: type 1 diabetes, diabetes B, Idiotype diabetes and gestational diabetes mellitus.Though the various onset diabetes causes of disease, pathogenesis are different, are main common trait with hyperglycaemia all.
Human body euglycemia concentration is at 0.60～0.95 mg/ml.Fast blood sugar monitoring is very important for the diabetic easily.Use that insulin treats the diabetic because need to confirm insulin dose, need every day 3～10 times blood sugar concentration to detect.
Multiple blood sugar test paper is arranged in market at present; The Accu-Check Aviva of Roche Holding Ag for example, the OneTouch Ultra2 of Johson & Johnson, the FreeStyle Freedom of Abbott Laboratories company limited; The Ascensia Contou test paper of Beyer Co., Ltd; When selecting for use above-mentioned blood sugar test paper to detect blood sugar concentration, need be equipped with special electrochemical signals pick-up unit, just blood glucose meter.Usually, before detecting blood sugar concentration, also need proofread and correct blood glucose meter.Because detecting the principle of blood sugar concentration is electrochemical principle, promptly through detecting the H that generates in the glucose oxidase process
2O
2Electrochemical signals confirm the concentration of glucose in the blood, the base material of test paper is polypropylene, tygon, PVC and polycarbonate plastic, its preparation method need be on plastic basis material test paper surface layer designing printed circuit.Because therefore the P.e.c. complex manufacturing technology is that the blood sugar test paper of base material exists preparation technology and test paper complex structure and expensive defective thereof with plastics.
Summary of the invention
The objective of the invention is to overcome the weak point that prior art exists, and a kind of visual blood sugar test paper is provided.This visual blood sugar test paper is a kind of special electrochemical signals pick-up unit that need not to use, and only needs to change through the test paper surface color, judges blood sugar concentration and carries out the quick detection of blood sugar with naked eyes.This visual blood sugar test paper stable performance, inexpensive.
Another object of the present invention is to provide a kind of preparation method of visual blood sugar examination, this method technology is simple, and operation easy to implement, thereby has solved the defective that prior art exists.
For realizing the object of the invention, the technical scheme that is adopted is:
A kind of visual blood sugar test paper is characterized in that: its structure scribbles compound composition of Millipore nanofiltration membrane of bonding agent by saturating paper base material and bottom surface, wherein:
Said saturating paper base material, its use body paper by one or more paper pulp in bleach chemical pulp, bleached chemi-mechanical pulp and the bleached mechanical pulp copy system, be 20 g/m quantitatively
2～250 g/m
2Paper, optional wherein a kind of, said quantitatively preferred condition is 30 g/m
2～120 g/m
2
Said bottom surface scribbles the Millipore nanofiltration membrane of bonding agent, and wherein: said bonding agent is 3 g/m
2～10 g/m
2Poly (vinyl alcohol) binder, or 3 g/m
2～10 g/m
2Gelatin adhesive, or 2 g/m
2～6g/m
2Vinyl acetate binder, optional wherein a kind of;
Said Millipore nanofiltration membrane is the Millipore 150 nm filter membranes of aperture less than the low protein adsorption of 500 nm, or Millipore 250 nm filter membranes, and is optional wherein a kind of.
A kind of preparation method of visual blood sugar test paper; It is characterized in that: the preparation method comprises one), the preparation of impregnant solution and the preparation and two of saturating paper base material), that saturating paper base material and bottom surface scribble the Millipore nanofiltration membrane of bonding agent is compound, concrete operations are following:
One), the preparation of the preparation of impregnant solution and saturating paper base material
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and is used to regulate the buffer solution of impregnant pH value of solution value, wherein:
Said enzyme is glucose oxidase and HRPO;
Said chromogen is 2,4; 6-three bromo-3-hydroxybenzoic acids, 2,4,6-three bromo-3-hydroxy benzene sulfonic acids, 2; 4-two chloro-6-bromo-3-hydroxy-benzoic acids, 2,4-two fluoro-6-bromo-3-hydroxy-benzoic acids, 2,4-two chloro-6-bromo-3-hydroxyl-benzene sulfonic acids and 2; In 4-two fluoro-6-bromo-3-hydroxyl-benzene sulfonic acids one or more, optional wherein a kind of;
Said auxiliary developer is 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1, and one or more in 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone, optional wherein a kind of;
The agent of said liquid diffusion control, for polyvinyl alcohol (PVA), molecular weight at one or more of the shitosan below 10000, guar gum and gelatin, optional wherein a kind of; The massfraction of the said liquid diffusion control agent WS is 0.2%～10%;
The material proportion of the auxiliary developer of chromogen and auxiliary developer is 1:0.1～3.0 in the said impregnant solution, g/g;
The material proportion of chromogen and the agent of liquid diffusion control is in the said impregnant solution: 1:1～10, g/g;
The said buffer solution that is used to regulate impregnant pH value of solution value is one or more of citric acid/sodium citrate, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum, and is optional wherein a kind of;
2. the preparation of impregnant solution
Prepare the WS of the WS, the chromogen WS, the auxiliary developer WS and the agent of liquid diffusion control of enzyme respectively; With above-mentioned aqueous solution; The buffer solution adjusting impregnant pH value of solution value that adding is used to regulate impregnant pH value of solution value gets impregnant solution between 3～5, keep in Dark Place;
2), the preparation of saturating paper base material
The body paper that the saturating paper base material is used cuts into paper slip, gets impregnant solution and is contained in the container, paper slip is placed immerse impregnant solution in the container; Make impregnant solution fully immerse page inside, the dip treating temperature range is 3 ℃～60 ℃, and preferred condition is at 15 ℃～40 ℃; Soak time is 1.0 min～20 min, after the immersion, takes out; Dry under 5 ℃～40 ℃, make the saturating paper base material;
Two) it is compound that, saturating paper base material and bottom surface scribble the Millipore nanofiltration membrane of bonding agent
It is compound for one through the adhesive effect of bonding agent that saturating paper base material and bottom surface scribble the Millipore nanofiltration membrane of bonding agent, makes visual blood sugar test paper of the present invention.
Advantage and beneficial effect
Compare with the blood sugar test paper of polypropylene, tygon, PVC and polycarbonate substrate, the advantage of visual blood sugar test paper of the present invention and beneficial effect, respectively as follows:
Advantage
1), in blood sugar test, need not to use to be equipped with special electrochemical signals pick-up unit, only need to change through the test paper surface color, judge blood sugar concentration with naked eyes, have the effect of quick detection blood sugar.
2), stable performance, at room temperature at least 3 months holding times.
3), inexpensive: about 3 yuan～5 yuan/bar of commercially available plastic based blood sugar test paper price, visual blood sugar test paper price of the present invention is merely 5% of commercially available plastic based blood sugar test paper price.
Beneficial effect
1), the present invention is with the body paper of plant fiber paper as the saturating paper base material, is a kind of natural resources of reproducibility, has favorable biological degradability.
2), compare with plastic basis material, the low price of saturating paper sill has clear superiority.
3), the saturating paper base material has exclusive vegetable fibre structure, is a kind of desirable carrier in glucose detection.Through membrane filtration blood, utilize chromogen reagent, help developer and H
2O
2Chromogenic reaction, have the beneficial effect of measuring blood glucose value fast.
Description of drawings
Fig. 1 is the structural representation of the visual blood sugar test paper of the present invention;
Fig. 2 changes synoptic diagram for its surface reflectivity of saturating paper base material colour developing back;
Fig. 3 is the relation curve of its surface reflectivity variation of saturating paper base material colour developing back with concentration of glucose (log [C]);
Fig. 4 is difference Dropwise 5 .0 on the saturating paper base material
mThe glucose standard solution colour developing of L variable concentrations, colour developing back picture;
Among Fig. 1: the 1-Millipore nanofiltration membrane; The 2-bonding agent; 3-saturating paper base material.
Embodiment
In order to make the object of the invention, technical scheme and advantage clearer,, the present invention is further elaborated below in conjunction with accompanying drawing.Should be appreciated that specific embodiment described herein only in order to explanation the present invention, and be not used in qualification the present invention.
A kind of visual blood sugar test paper scribbles 3 compound compositions of Millipore nanofiltration membrane of bonding agent 2 by saturating paper base material 1 and bottom surface, wherein:
Said saturating paper base material 1, its use body paper by one or more paper pulp in bleach chemical pulp, bleached chemi-mechanical pulp and the bleached mechanical pulp copy system, be 20 g/m quantitatively
2～250 g/m
2It quantitatively is 40 g/m that paper, present embodiment are chosen what copy system by bleach chemical pulp
2Paper;
Said bottom surface scribbles the Millipore nanofiltration membrane 2 of bonding agent 2, and wherein: said bonding agent 2 is 3 g/m
2～10 g/m
2Poly (vinyl alcohol) binder, or 3 g/m
2～10 g/m
2Gelatin adhesive, or 2 g/m
2～6g/m
2Vinyl acetate binder, present embodiment are chosen 3 g/m
2Poly (vinyl alcohol) binder; Said Millipore nanofiltration membrane 3 is the Millipore 150 nm filter membranes of aperture less than the low protein adsorption of 500 nm, or Millipore 250 nm filter membranes; Present embodiment design Millipore 150 nm filter membranes are Mi Libo (Shanghai) trade Co., Ltd product.
A kind of preparation method of visual blood sugar test paper comprises one), the preparation of impregnant solution and the preparation and two of saturating paper base material 1), saturating paper base material 1 is compound with the Millipore nanofiltration membrane 3 that scribbles bonding agent 2, concrete operations are following:
One), the preparation of the preparation of impregnant solution and saturating paper base material 1
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and is used to regulate the buffer solution of maceration extract pH value, wherein:
Said enzyme is glucose oxidase and peroxidase;
Said chromogen is 2,4,6-three bromo-3-hydroxybenzoic acids, 2; 4,6-three bromo-3-hydroxy benzene sulfonic acids, 2,4-two chloro-6-bromo-3-hydroxy-benzoic acids, 2,4-two fluoro-6-bromo-3-hydroxy-benzoic acids, 2; 4-two chloro-6-bromo-3-hydroxyl-benzene sulfonic acids and 2, one or more in 4-two fluoro-6-bromo-3-hydroxyl-benzene sulfonic acids, present embodiment chooses 2,4; 6-three bromo-3-hydroxybenzoic acids and 2,4, the 6-three bromo-3-hydroxy benzene sulfonic acid WS;
Said auxiliary developer; Be 1; 5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1; In 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone one or more, present embodiment chooses 1, the 5-dimethyl-2-phenyl-4-amino-3-pyrazolone WS;
The material proportion of the auxiliary developer of chromogen and auxiliary developer is 1:0.1～3.0 in the said impregnant solution, g/g;
The material proportion of chromogen and the agent of liquid diffusion control is in the said impregnant solution: 1:1～10, g/g;
The agent of said liquid diffusion control is polyvinyl alcohol (PVA), and molecular weight is at the shitosan below 10000, one or more of guar gum and gelatin, and present embodiment is chosen polyvinyl alcohol water solution; The massfraction of the said liquid diffusion control agent WS is 0.2%～10%;
The said buffer solution that is used to regulate impregnant pH value of solution value, for citric acid/sodium citrate, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum one or more, present embodiment is chosen lemon acid/sodium citrate
2. the preparation of impregnant solution
Measure the 2.5 ml enzyme mixed solutions that contain 16.2 mg glucose oxidases and 1.2 mg horseradish peroxidases in polyvinyl alcohol water solution and the per 10 ml enzyme mixed solutions of 12.0 ml 5% respectively; And 20.0 ml, 5 mg/ml 2,4, the 6-three bromo-3-hydroxybenzoic acid WS; 5.0 1 of ml 20 mg/ml; 5-dimethyl-2-phenyl-4-amino-3-pyrazolone the WS mixes above solution, and the pH value that adds 10.5 ml citric acid/sodium citrate buffer solution modulation impregnant solution is between 3-5; Prepare impregnant solution, keep in Dark Place;
2), the preparation of saturating paper base material 1
The body paper of saturating paper base material 1 usefulness is cut 10 of the paper slips of 4.0 cm * 2.5 cm specifications, get maceration extract 5 ml and be contained in the container, paper slip is placed immerse impregnant solution in the container; Make impregnant solution fully immerse page inside, the dip treating temperature range is 15 ℃～60 ℃, and present embodiment is designed to 15 ℃; Soak time is 1.0 min～20 min; Present embodiment dip treating temperature design is 5.0 min, soaks the back and takes out, and is dry down at 5 ℃～40 ℃; The present embodiment baking temperature is designed to 10 ℃, makes saturating paper base material 1;
Two), saturating paper base material 1 is compound with the Millipore nanofiltration membrane 3 that the bottom surface scribbles bonding agent 2
Saturating paper base material 1 is compound for one through the adhesive effect of bonding agent with the Millipore nanofiltration membrane 3 that the bottom surface scribbles bonding agent 2, makes visual blood sugar test paper of the present invention;
Said Millipore nanofiltration membrane 3 is Mi Libo (Shanghai) trade Co., Ltd product.
The agent of liquid diffusion control is the aqueous guar glue solution of 2.0 ml 3%;
1.5 ml enzyme (containing 16.2 mg glucose oxidases and 1.2 mg horseradish peroxidases in per 10 ml enzyme mixed solutions);
Regulating the buffer solution of impregnant pH value of solution, is 11.5 ml citric acid/sodium citrate buffer solution.
Regulating the buffer solution of impregnant pH value of solution, is 11.5 ml malic acid/natrium malicum buffer solution.
Embodiment 4
The agent of liquid diffusion control is the polyvinyl alcohol water solution of 12.0 ml 6%;
1.5 ml enzyme (containing 16.2 mg glucose oxidases and 1.2 mg horseradish peroxidases in per 10 ml enzyme mixed solutions),
Regulating the buffer solution of impregnant pH value of solution, is 11.5 ml acetic acid/sodium acetate buffer solution.
Saturating paper substrate surface Show Color performance of the present invention:
1), the mensuration of Show Color performance
Adopt Datacplor ElREPHO 4230 spectrophotometric color measurement instruments to measure the reflectivity on saturating paper base material 1 surface, observe visual blood sugar test paper surface Show Color performance variation, concrete operations are following:
On dried saturating paper base material 1, dripping concentration respectively is 5 of 0.72,1.80,6.30 mg/ml
μThe glucose solution of l, behind 1 min that develops the color, with the reflectivity on Datacolor ELREPHO 4230 spectrophotometric color measurement instruments mensuration saturating paper base material 1 surface, the variation of reflectivity was as shown in Figure 1 before and after saturating paper base material 1 showed.
Saturating paper base material 1 shows that the back reflection rate changes and the relation curve of concentration of glucose (log [C]), and is as shown in Figure 2.
2), after saturating paper base material 1 colour developing, the stability of change color response
Get the saturating paper base material 1 that embodiment 4 obtains, Dropwise 5 .0
mThe glucose solution colour developing of 4.5 mg/ml of L is measured its surperficial reflectance varies with Datacolor ELREPHO 4230 spectrophotometric color measurement instruments, and the response time is to the result that influences of saturating paper base material 1 colour developing back surface reflectivity variation, and is as shown in table 1.Can find out that from table 1 saturating paper base material 1 is between colour developing 1 min～10min, the change color response is stable.
Table 1
| Response time | ΔR | Response time | ΔR |
| 30 second | -13.29 | 20 min | -18.35 |
| 1 min | -18.33 | 40 min | -18.36 |
| 2 min | -18.33 | 60 min | -18.38 |
| 3 min | -18.33 | 80 min | -18.42 |
| 10 min | -18.33 | 100 min | -18.45 |
3), test paper stability
Get the saturating paper base material 1 that embodiment 4 obtains, and scribble 10 g/m with the bottom surface
2The Millipore 150 nm filter membranes 2 of gelatin are compound, obtain test paper, under different condition, preserve, and detect the test paper performance with standard glucose solution (4.5 mg/ml), and testing result is as shown in table 2.Can find out that from table 2 the gained test paper has good stability, at room temperature can preserve at least 3 months.
Table 2.
4), saturating paper base material Show Color effect
Get the saturating paper base material 1 that embodiment 4 obtains, distinguish Dropwise 5 .0 above that
mThe glucose standard solution colour developing of L variable concentrations, colour developing back picture is seen Fig. 4.
The principle of the visual blood sugar test paper of the present invention and application thereof
Principle:
The principle of the visual blood sugar test paper test of the present invention blood sugar is through filter membrane 2 filtering bloods, utilizes chromogen reagent, helps developer and H
2O
2Chromogenic reaction, its course of reaction is following:
Use:
In the process that adopts test paper test blood sugar of the present invention, the test paper surface demonstrates deep mixed red color blood concentration and indicates, and each red color that differs from shallow to deep indicates the height extent value that shows the blood sugar concentration of testing.
With the hyperglycaemia is the diabetes of principal character, and its incidence of disease is remarkable ascendant trend in recent years, and the whole world has 10% adult to suffer from this disease approximately at present.2011, nearly 9,000 ten thousand people of China diabetic, wherein 18 years old and above resident's diabetes prevalence are about 9.7%, and China 18 years old and above resident's diabetes awareness are merely 36.1%.China diabetes patient also increases in the speed with annual 1.5%.The visual blood sugar test paper of the blood sugar test of a kind of cheap, easy to use, the dependable performance of the present invention exploitation for China of diabetes high incidence, detects in diabetes, and diagnosis and therapeutic process have important practical significance.
According to ADA statistics, the U.S. is that the sales volume to 2009 of all Related products of master year reaches 97.32 hundred million dollars with diabetes test articles for use, and approximately increase progressively 11.2% every year.The total sales volume of the blood sugar test articles for use of China in 2010 surpasses 500,000,000 dollars, and therefore visual blood sugar test paper of the present invention can be China diabetes test articles for use market and creates considerable economic benefit.
The above only is a preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from design concept of the present invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (2)
1. visual blood sugar test paper is characterized in that: its structure scribbles compound composition of Millipore nanofiltration membrane (3) of bonding agent (2) by saturating paper base material (1) and bottom surface, wherein:
Said saturating paper base material (1), its body paper by one or more paper pulp in bleach chemical pulp, bleached chemi-mechanical pulp and the bleached mechanical pulp copy system, be 20 g/m quantitatively
2～250 g/m
2Paper, optional wherein a kind of, said quantitatively preferred condition is 30 g/m
2～120 g/m
2
Said bottom surface scribbles the Millipore nanofiltration membrane (3) of bonding agent (2), and wherein: said bonding agent (2) is 3～10 g/m
2Poly (vinyl alcohol) binder, or 3～10 g/m
2Gelatin adhesive, or 2 g/m
2～6g/m
2Vinyl acetate binder, optional wherein a kind of;
Said Millipore nanofiltration membrane (3) is the Millipore 150 nm filter membranes of aperture less than the low protein adsorption of 500 nm, or Millipore 250 nm filter membranes, and is optional wherein a kind of.
2. realize the preparation method of the said a kind of visual blood sugar test paper of claim 1; It is characterized in that: the preparation method comprises one), the preparation of impregnant solution and the preparation and two of saturating paper base material (1)), that saturating paper (1) base material and bottom surface scribble the Millipore nanofiltration membrane (3) of bonding agent (2) is compound, concrete operations are following:
One), the preparation of the preparation of impregnant solution and saturating paper base material (1)
1), the preparation of impregnant solution
1. the component of impregnant solution:
Impregnant solution contains enzyme, chromogen, auxiliary developer, the agent of liquid diffusion control and is used to regulate the buffer solution of impregnant pH value of solution value, wherein:
Said enzyme is glucose oxidase and HRPO;
Said chromogen is 2,4; 6-three bromo-3-hydroxybenzoic acids, 2,4,6-three bromo-3-hydroxy benzene sulfonic acids, 2; 4-two chloro-6-bromo-3-hydroxy-benzoic acids, 2,4-two fluoro-6-bromo-3-hydroxy-benzoic acids, 2,4-two chloro-6-bromo-3-hydroxyl-benzene sulfonic acids and 2; In 4-two fluoro-6-bromo-3-hydroxyl-benzene sulfonic acids one or more, optional wherein a kind of;
Said auxiliary developer is 1,5-dimethyl-2-phenyl-4-amino-3-pyrazolone, 1, and one or more in 5-diethyl-2-phenyl-4-amino-3-pyrazolone and 1-methyl-5-ethyl-2-phenyl-4-amino-3-pyrazolone, optional wherein a kind of;
The agent of said liquid diffusion control, for polyvinyl alcohol (PVA), molecular weight at one or more of the shitosan below 10000, guar gum and gelatin, optional wherein a kind of; The massfraction of the said liquid diffusion control agent WS is 0.2%～10%;
Chromogen and auxiliary developer material proportion are 1:0.1～3.0 in the said impregnant solution, g/g;
The material proportion of chromogen and the agent of liquid diffusion control is in the said impregnant solution: 1:1～10, g/g;
The said buffer solution that is used to regulate impregnant pH value of solution value is one or more of citric acid/sodium citrate, acetic acid/sodium acetate, tartrate/sodium tartrate, malic acid/natrium malicum, and is optional wherein a kind of;
2. the preparation of impregnant solution
Prepare the WS of the WS, the chromogen WS, the auxiliary developer WS and the agent of liquid diffusion control of enzyme respectively; With above-mentioned aqueous solution; The buffer solution adjusting impregnant pH value of solution value that adding is used to regulate impregnant pH value of solution value gets impregnant solution between 3～5, keep in Dark Place;
2), the preparation of saturating paper base material (1)
The body paper of saturating paper base material (1) usefulness is cut into paper slip, get impregnant solution and be contained in the container, paper slip is placed immerse impregnant solution in the container; Make impregnant solution fully immerse page inside, the dip treating temperature range is 3 ℃～60 ℃, and preferred condition is at 15 ℃～40 ℃; Soak time is 1.0 min～20 min, after the immersion, takes out; Dry under 5 ℃～40 ℃, make impregnated paper base material (1);
Two), to scribble bonding agent (2) Millipore nanofiltration membrane (3) compound saturating paper base material (1) and bottom surface
Saturating paper base material (1) is compound for one through the adhesive effect of bonding agent with the Millipore filter membrane (3) that the bottom surface scribbles bonding agent (2), makes visual blood sugar test paper of the present invention.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104749169A (en) * | 2013-12-31 | 2015-07-01 | 比亚迪股份有限公司 | Blood glucose testing device and blood glucose testing kit |
| CN104749171A (en) * | 2013-12-31 | 2015-07-01 | 比亚迪股份有限公司 | Blood glucose detection device and preparation method thereof |
| CN105353116A (en) * | 2015-11-09 | 2016-02-24 | 国家纳米科学中心 | Method for immunoassay based on hydrogen peroxide test strip and applications |
| CN111595839A (en) * | 2020-05-19 | 2020-08-28 | 中科康磁医疗科技（苏州）有限公司 | Saliva glucose detection test paper, preparation method and application thereof |
| CN114107436A (en) * | 2021-11-26 | 2022-03-01 | 华南理工大学 | Nano-cellulose/wood fiber composite paper-based saliva glucose detection chip |
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| CN104749169A (en) * | 2013-12-31 | 2015-07-01 | 比亚迪股份有限公司 | Blood glucose testing device and blood glucose testing kit |
| CN104749171A (en) * | 2013-12-31 | 2015-07-01 | 比亚迪股份有限公司 | Blood glucose detection device and preparation method thereof |
| CN104749169B (en) * | 2013-12-31 | 2018-04-20 | 比亚迪股份有限公司 | Blood sugar detection apparatus and blood sugar test kit |
| CN104749171B (en) * | 2013-12-31 | 2018-06-26 | 比亚迪股份有限公司 | Blood sugar detection apparatus and preparation method thereof |
| CN105353116A (en) * | 2015-11-09 | 2016-02-24 | 国家纳米科学中心 | Method for immunoassay based on hydrogen peroxide test strip and applications |
| CN111595839A (en) * | 2020-05-19 | 2020-08-28 | 中科康磁医疗科技（苏州）有限公司 | Saliva glucose detection test paper, preparation method and application thereof |
| CN114107436A (en) * | 2021-11-26 | 2022-03-01 | 华南理工大学 | Nano-cellulose/wood fiber composite paper-based saliva glucose detection chip |
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| CN102520182B (en) | 2014-01-15 |
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