CN103484353A - Biomacromolecule extracting device based on filter paper - Google Patents
Biomacromolecule extracting device based on filter paper Download PDFInfo
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- CN103484353A CN103484353A CN201210193723.9A CN201210193723A CN103484353A CN 103484353 A CN103484353 A CN 103484353A CN 201210193723 A CN201210193723 A CN 201210193723A CN 103484353 A CN103484353 A CN 103484353A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/087—Multiple sequential chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0874—Three dimensional network
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
Abstract
The invention discloses a biomacromolecule extracting device based on a filter paper. The device is composed of an upper-layer substrate (1), a lower-layer substrate (3) and the filter paper (2) sandwiched between the upper-layer substrate and the lower-layer substrate, the upper-layer substrate and the lower-layer substrate are stacked to each other, and a biomacromolecule extracting structure unit having the following structure as shown in the specification is formed between the upper-layer and lower-layer substrates and the filter paper. The upper-layer substrate is provided with sample entering holes (4) and a sample exiting hole (7); the sample entering holes are communicated with the upper surface of the filter paper through an upper flow guiding groove (5) arranged on the lower surface of the upper-layer substrate; the sample exiting hole is communicated with the lower surface of the filter paper through a lower flow guiding groove (6) arranged on the upper surface of the lower-layer substrate; the filter paper is located at a region where the upper flow guiding groove and the lower flow guiding groove intersect, and the upper flow guiding groove and the lower flow guiding groove are communicated with each other only through the filter paper. The device is used for extracting biomacromolecules, and has the advantages of high efficiency, speediness, low biological sample consumption, low cost, easy operation and the like. The device has great significance of realizing a micro total analysis system and promoting gene analysis for further popularization and application.
Description
Technical field
The present invention relates to a kind of biomacromolecule extraction element based on filter paper.
Background technology
Along with the development with DNA sequencing technology of future generation that is successfully completed of human genome order-checking project, gene analysis test just more and more obtains people's attention, and progressively is applied to the various fields such as health care, environment protection and judicial expertise.For further promoting the development and application of genetic analysis, realize the low cost of whole testing process, fast and automatization be very necessary.And microflow control technique has advantageous advantage in the development that promotes genetic analysis, and obtain significant progress.
Usually the analytic process of nucleic acid comprises following three steps: the enzymatic amplification reaction of nucleic acid extraction, target sequence and three steps of separation detection of product.Wherein, very important as nucleic acid extraction and the purifying of the first step, it has directly determined the success or not of subsequent process.
Traditional method for extracting nucleic acid has two kinds: the one, and the phenol chloroform extraction method, two are based on the various forms of method for extracting nucleic acid of Solid-Phase Extraction.
The phenol chloroform extraction method has advantage with low cost, but owing to relating to poisonous harmful reagent and complex operation, and is not suitable for being transplanted on micro-fluidic chip and carries out.
Various forms of method for extracting nucleic acid based on Solid-Phase Extraction are mainly that purifying is realized extracting in the surface that nucleic acid molecule is adsorbed on to the solid matters such as silicon-dioxide, glass, diatomite.At present a lot of commercially available test kits are all taked the method, and its form also comprises spe membrane, column extractor, microballoon, powder and magnetic bead etc.The method is suitable for being transplanted to microfluidic platforms comparatively speaking, and the various micro-fluidic chips based on this principle are developed in succession.But unquestionable, these sample preparation chips still have the place of a lot of deficiencies, as high as cost, procedure of processing is loaded down with trivial details, complex operation etc.
Low-cost, quick and easy nucleic acid samples prepares the study hotspot that chip remains people, and its research and development, to realizing micro-full analytical system, promote the tool of further applying of genetic analysis to be of great significance.
Summary of the invention
The purpose of this invention is to provide a kind of biomacromolecule extraction element based on filter paper.
Biomacromolecule extraction element provided by the invention (device first), it is characterized in that: it by upper strata substrate 1 and lower floor's substrate 3 of closed assembly and be clamped in described upper strata substrate and described lower floor substrate between filter paper 2 form, form at least one biomacromolecule between described upper strata substrate, described lower floor substrate and filter paper and extract structural unit;
The structure that described biomacromolecule is extracted structural unit is as follows: described upper strata substrate is provided with at least one sample holes run through 4 and a sample outlet hole run through 7; The upper diversion trench 5 of the lower surface of described sample holes by being located at described upper strata substrate is communicated with the upper surface of described filter paper; The lower diversion trench 6 of the upper surface of described sample outlet hole by being located at described lower floor substrate is communicated with the lower surface of described filter paper; Described filter paper is positioned at the intersectional region of described upper diversion trench and described lower diversion trench, between described upper diversion trench and described lower diversion trench, only by described filter paper, communicates.
Described upper diversion trench and described lower diversion trench are the microfluid pipeline.
The width of described upper diversion trench and described lower diversion trench is 0.005-50 millimeter (as 1 millimeter), and the degree of depth is 0.005-50 millimeter (as 0.5 millimeter).
Described upper diversion trench is corresponding away from the shape of the shape of an end of described sample holes and described filter paper; Described lower diversion trench is corresponding away from the shape of the shape of an end of described sample outlet hole and described filter paper; The shape of one end of the close described sample outlet hole of described lower diversion trench is corresponding with the shape of described sample outlet hole.
Specifically can be provided with 3 described sample holes on the substrate of described upper strata.
The present invention also protects another kind of biomacromolecule extraction element (device second), it is characterized in that: it by upper strata substrate and lower floor's substrate of closed assembly and be clamped in described upper strata substrate and described lower floor substrate between filter paper form, form at least one biomacromolecule between described upper strata substrate, described lower floor substrate and filter paper and extract structural unit;
The structure that described biomacromolecule is extracted structural unit is as follows: described upper strata substrate is provided with a sample holes run through and a sample outlet hole run through; Described sample holes is communicated with the upper surface of described filter paper; The diversion trench of the upper surface of described sample outlet hole by being located at described lower floor substrate is communicated with the lower surface of described filter paper; Described filter paper is positioned at the intersectional region of described sample holes and described diversion trench, between described sample holes and described diversion trench, only by described filter paper, communicates.
Described diversion trench is the microfluid pipeline.
The width of described diversion trench is the 0.005-50 millimeter, and the degree of depth is the 0.005-50 millimeter.
Described diversion trench is corresponding away from the shape of the shape of an end of described sample outlet hole and described filter paper; The shape of one end of the close described sample outlet hole of described diversion trench is corresponding with the shape of described sample outlet hole.
In described device first and/or described device second, the thickness of described filter paper can be 0.01-5 millimeter (as 0.15 millimeter).
Described biomacromolecule specifically can be nucleic acid.Described nucleic acid specifically can be Yeast Nucleic Acid or thymus nucleic acid.
Any one in specifically can be by the following method is held in described filter holder between described upper strata substrate and described lower floor: hot pressing, tackiness agent are bonding, physical bond or chemical bonding.
Any one in specifically can be by the following method is stacked described two substrates: hot pressing, tackiness agent is bonding and double sticky tape is bonding.
The cross section of described upper diversion trench specifically can be rectangle, can be also any other geometrical shape.The cross section of described lower diversion trench specifically can be rectangle, can be also any other geometrical shape.The cross section of described diversion trench specifically can be rectangle, can be also any other geometrical shape.
The shape of described filter paper can be circle, ellipse, square or rhombus etc.When described filter paper is circle, its diameter can be 0.5-50 millimeter (as 10 millimeters).
Described filter paper is the filter paper that has the filter paper of reticular fiber structure or have membrane structure.The aperture of described filter paper specifically can be the 0.20-200 micron, as 20 microns.
The material of described substrate can be at least one in following material: silicon, pottery, glass, plastics, silicone resin and resin.
Common plastic material comprises: polyamide (PA), polybutylene terephthalate (PBT), polycarbonate (PC), polyethylene (PE), polymethylmethacrylate (PMMA), polyoxymethylene (POM), polypropylene (PP), polystyrene diethyl ether (PPE), polystyrene (PS), polysulfones (PSU), polyether-ether-ketone (PEEK), polydimethylsiloxane (PDMS), cyclic olefine copolymer (COC), silicone resin (Silicone) etc.
Described upper diversion trench can be to be processed by any modes such as micro-processing or machinings.According to the difference of substrate for use material, its working method is also different, and silicon, glass and pottery can be processed by various wet methods or dry etching, and plastics, resin etc. can be processed by methods such as cast, mold pressing, etching or machinings.Described lower diversion trench can be to be processed by any modes such as micro-processing or machinings.According to the difference of substrate for use material, its working method is also different, and silicon, glass and pottery can be processed by various wet methods or dry etching, and plastics, resin etc. can be processed by methods such as cast, mold pressing, etching or machinings.Described diversion trench can be to be processed by any modes such as micro-processing or machinings.According to the difference of substrate for use material, its working method is also different, and silicon, glass and pottery can be processed by various wet methods or dry etching, and plastics, resin etc. can be processed by methods such as cast, mold pressing, etching or machinings.
Adopt device provided by the invention to extract biomacromolecule, there are efficient, the advantages such as quick, the biological specimen consumption is low, with low cost, easy handling.Simultaneously, device provided by the invention also is easy to be integrated and connected with downstream biochemical analysis chip (as pcr chip and capillary electrophoresis chip etc.).The present invention, for realizing micro-full analytical system, promotes the tool of further applying of genetic analysis to be of great significance.
In device provided by the invention, a plurality of self-existent biomacromolecules can be set and extract structural unit, make it form array, thereby realize high-throughput operation simultaneously prepared by a plurality of samples.
The accompanying drawing explanation
The structural representation that Fig. 1 is the biomacromolecule extraction element.
The exploded view that Fig. 2 is the biomacromolecule extraction element.
The principle of work schematic diagram that Fig. 3 is the biomacromolecule extraction element.
The agarose gel electrophoresis figure that Fig. 4 is pcr amplification product.
Embodiment
Following embodiment is convenient to understand better the present invention, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, be ordinary method.Test materials used in following embodiment, if no special instructions, be and purchase available from routine biochemistry reagent shop.
Embodiment 1,
The structural representation of the biomacromolecule extraction element adopted in the present embodiment is shown in Fig. 1, and exploded view is shown in Fig. 2.The biomacromolecule extraction element by upper strata substrate 1 and lower floor's substrate 3 of closed assembly and be clamped in described upper strata substrate and described lower floor substrate between filter paper 2 form, form at least one biomacromolecule between described upper strata substrate, described lower floor substrate and filter paper and extract structural unit.The structure that described biomacromolecule is extracted structural unit is as follows: the upper strata substrate is provided with three sample holes that run through (called after sample holes first, sample holes second and sample holes third respectively) and a sample outlet hole run through 7.Three sample holes all upper diversion trench 5 of the lower surface by being located at the upper strata substrate are communicated with the upper surface of filter paper.The lower diversion trench 6 of the upper surface of sample outlet hole by being located at lower floor's substrate is communicated with the lower surface of described filter paper; Described filter paper is positioned at the intersectional region of described upper diversion trench and described lower diversion trench, between described upper diversion trench and described lower diversion trench, only by described filter paper, communicates.Sample outlet hole is from outside connecting needle pump.
In the present embodiment, described filter paper is clamped between described upper strata substrate and described lower floor substrate by hot pressing, described two substrates are stacked by hot pressing, described upper diversion trench and described lower diversion trench be all adopt the method processing of etching to obtain, cross section all rectangular, width be 1 millimeter, the degree of depth and be 0.5 millimeter, the be shaped as circle (its diameter is 10 millimeters), thickness of described filter paper are that 0.15 millimeter, aperture are 20 microns, and the material of described upper strata substrate and described lower floor substrate is plastics (polymethylmethacrylate).
When the device that application the present embodiment provides extracts nucleic acid, externally under the effect of negative pressure device (pin pump), successively will be for extracting the biological specimen (liquid form) of nucleic acid (DNA or RNA), lysate and scavenging solution add the through hole first, in through hole second and through hole third, externally under the effect of negative pressure, all liquid flows through filter paper successively, the reticular fiber structure that utilizes filter paper to the winding blocking effect of long-chain nucleic acid molecule by its separation and Extraction out (Fig. 3 is shown in by the principle of work schematic diagram) from biological sample, filter paper can be taken out and carries out subsequent operations (as pcr amplification, separation detection etc.), can also in device, directly carry out described subsequent operations.
Biological specimen for the present embodiment is: human blood.
1, biological specimen is added to the biomacromolecule extraction element by the sample holes first, under the effect of pin pump by diversion trench arrive and be adsorbed on filter paper, other liquid flows out from sample outlet hole.
2, cell pyrolysis liquid is added to the biomacromolecule extraction element by sample holes second, on passing through under the effect of pin pump, diversion trench arrives filter paper, under the effect of cell pyrolysis liquid, cell generation cracking in biological specimen, discharge nucleic acid molecule, nucleic acid molecule is adsorbed on filter paper, and other liquid flows out from sample outlet hole.
Cell pyrolysis liquid: the 10mM NaOH aqueous solution.
3, water is added to the biomacromolecule extraction element by sample holes third, diversion trench, filter paper on passing through successively under the effect of pin pump, lower diversion trench also flows out from sample outlet hole, completes the cleaning to nucleic acid molecule.
4, take out filter paper from the biomacromolecule extraction element, lay the disk of 2 millimeters with punch tool, directly as template, carry out pcr amplification.
Pcr amplification primer used is as follows:
Upstream primer: 5 '-CCCTGGGCTCTGTAAAGAA-3 ';
Downstream primer: 5 '-ATCAGAGCTTAAACTGGGAAGCTG-3 '.
The target sequence of pcr amplification is the Amelogenin gene fragment in human genome, as shown in the sequence 1 of sequence table.
The agarose gel electrophoresis figure of pcr amplification product is shown in Fig. 4, wherein, DL2000 is molecular weight standard, the negative contrast of NC (take water as template), the positive contrast of PC (double-stranded DNA shown in sequence 1 of take is template), sample 1 to sample 3 for three biological specimens are tested.Result shows, adopts device of the present invention to extract the template that the DNA obtained can be used as pcr amplification fully.
Claims (10)
1. a biomacromolecule extraction element, it is characterized in that: it by upper strata substrate (1) and lower floor's substrate (3) of closed assembly and be clamped in described upper strata substrate and described lower floor substrate between filter paper (2) form, form at least one biomacromolecule between described upper strata substrate, described lower floor substrate and filter paper and extract structural unit;
The structure that described biomacromolecule is extracted structural unit is as follows: described upper strata substrate is provided with at least one sample holes run through (4) and a sample outlet hole run through (7); The upper diversion trench (5) of the lower surface of described sample holes by being located at described upper strata substrate is communicated with the upper surface of described filter paper; The lower diversion trench (6) of the upper surface of described sample outlet hole by being located at described lower floor substrate is communicated with the lower surface of described filter paper; Described filter paper is positioned at the intersectional region of described upper diversion trench and described lower diversion trench, between described upper diversion trench and described lower diversion trench, only by described filter paper, communicates.
2. biomacromolecule extraction element as claimed in claim 1, it is characterized in that: described upper diversion trench and described lower diversion trench are the microfluid pipeline.
3. biomacromolecule extraction element as claimed in claim 1 or 2, it is characterized in that: the width of described upper diversion trench and described lower diversion trench is the 0.005-50 millimeter, and the degree of depth is the 0.005-50 millimeter.
4. as arbitrary described biomacromolecule extraction element in claims 1 to 3, it is characterized in that: described upper diversion trench is corresponding away from the shape of the shape of an end of described sample holes and described filter paper; Described lower diversion trench is corresponding away from the shape of the shape of an end of described sample outlet hole and described filter paper; The shape of one end of the close described sample outlet hole of described lower diversion trench is corresponding with the shape of described sample outlet hole.
5. a biomacromolecule extraction element, it is characterized in that: it by upper strata substrate and lower floor's substrate of closed assembly and be clamped in described upper strata substrate and described lower floor substrate between filter paper form, form at least one biomacromolecule between described upper strata substrate, described lower floor substrate and filter paper and extract structural unit;
The structure that described biomacromolecule is extracted structural unit is as follows: described upper strata substrate is provided with a sample holes run through and a sample outlet hole run through; Described sample holes is communicated with the upper surface of described filter paper; The diversion trench of the upper surface of described sample outlet hole by being located at described lower floor substrate is communicated with the lower surface of described filter paper; Described filter paper is positioned at the intersectional region of described sample holes and described diversion trench, between described sample holes and described diversion trench, only by described filter paper, communicates.
6. biomacromolecule extraction element as claimed in claim 5, it is characterized in that: described diversion trench is the microfluid pipeline.
7. biomacromolecule extraction element as described as claim 5 or 6, it is characterized in that: the width of described diversion trench is the 0.005-50 millimeter, the degree of depth is the 0.005-50 millimeter.
8. as arbitrary described biomacromolecule extraction element in claim 5 to 7, it is characterized in that: described diversion trench is corresponding away from the shape of the shape of an end of described sample outlet hole and described filter paper; The shape of one end of the close described sample outlet hole of described diversion trench is corresponding with the shape of described sample outlet hole.
9. as arbitrary described biomacromolecule extraction element in claim 1 to 8, it is characterized in that: the thickness of described filter paper is the 0.01-5 millimeter.
10. as arbitrary described biomacromolecule extraction element in claim 1 to 8, it is characterized in that: described biomacromolecule is nucleic acid.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210193723.9A CN103484353A (en) | 2012-06-12 | 2012-06-12 | Biomacromolecule extracting device based on filter paper |
| PCT/CN2013/000558 WO2013185467A1 (en) | 2012-06-12 | 2013-05-10 | Biomacromolecule retrieval device based on filter paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210193723.9A CN103484353A (en) | 2012-06-12 | 2012-06-12 | Biomacromolecule extracting device based on filter paper |
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| Publication Number | Publication Date |
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| CN103484353A true CN103484353A (en) | 2014-01-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210193723.9A Pending CN103484353A (en) | 2012-06-12 | 2012-06-12 | Biomacromolecule extracting device based on filter paper |
Country Status (2)
| Country | Link |
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| CN (1) | CN103484353A (en) |
| WO (1) | WO2013185467A1 (en) |
Cited By (6)
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| CN105482988A (en) * | 2016-01-14 | 2016-04-13 | 西安交通大学 | Paper-based full-automatic nucleic acid extraction device and preparation method |
| CN107841455A (en) * | 2016-09-21 | 2018-03-27 | 克雷多生物医学私人有限公司 | A kind of two benches operate nucleic acid reaction detection pipe |
| CN108085314A (en) * | 2016-11-21 | 2018-05-29 | 清华大学 | A kind of amination filter paper/film purified for nucleic acid extraction and preparation method and application |
| CN108384780A (en) * | 2018-02-11 | 2018-08-10 | 中国农业科学院烟草研究所 | A kind of method and extraction element of batch DNA rapid extraction |
| CN108499619A (en) * | 2018-03-09 | 2018-09-07 | 复旦大学 | A kind of integrated micro-fluidic filtrating chip of film and its preparation method and application |
| CN109735449A (en) * | 2019-03-08 | 2019-05-10 | 金婧菲 | A kind of online culture apparatus of biological tissue and observation method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105482988A (en) * | 2016-01-14 | 2016-04-13 | 西安交通大学 | Paper-based full-automatic nucleic acid extraction device and preparation method |
| CN105482988B (en) * | 2016-01-14 | 2017-12-08 | 西安交通大学 | A kind of full-automatic nucleic acid-extracting apparatus of paper substrate and preparation method |
| CN107841455A (en) * | 2016-09-21 | 2018-03-27 | 克雷多生物医学私人有限公司 | A kind of two benches operate nucleic acid reaction detection pipe |
| CN107841455B (en) * | 2016-09-21 | 2021-01-15 | 克雷多生物医学私人有限公司 | Two-stage operation nucleic acid reaction detection tube |
| CN108085314A (en) * | 2016-11-21 | 2018-05-29 | 清华大学 | A kind of amination filter paper/film purified for nucleic acid extraction and preparation method and application |
| CN108085314B (en) * | 2016-11-21 | 2021-11-09 | 杭州梓晶生物有限公司 | Aminated filter paper/membrane for nucleic acid extraction and purification and preparation method and application thereof |
| CN108384780A (en) * | 2018-02-11 | 2018-08-10 | 中国农业科学院烟草研究所 | A kind of method and extraction element of batch DNA rapid extraction |
| CN108384780B (en) * | 2018-02-11 | 2023-12-29 | 中国农业科学院烟草研究所 | Method and device for rapidly extracting DNA in batches |
| CN108499619A (en) * | 2018-03-09 | 2018-09-07 | 复旦大学 | A kind of integrated micro-fluidic filtrating chip of film and its preparation method and application |
| CN108499619B (en) * | 2018-03-09 | 2020-09-29 | 复旦大学 | Membrane integrated type micro-fluidic filter chip and preparation method and application thereof |
| CN109735449A (en) * | 2019-03-08 | 2019-05-10 | 金婧菲 | A kind of online culture apparatus of biological tissue and observation method |
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| Publication number | Publication date |
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| WO2013185467A1 (en) | 2013-12-19 |
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