CN103698276A - Fluorescence and ultraviolet-visible absorption integrated flow cell - Google Patents
Fluorescence and ultraviolet-visible absorption integrated flow cell Download PDFInfo
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- CN103698276A CN103698276A CN201310701299.9A CN201310701299A CN103698276A CN 103698276 A CN103698276 A CN 103698276A CN 201310701299 A CN201310701299 A CN 201310701299A CN 103698276 A CN103698276 A CN 103698276A
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- fluorescence
- optical fiber
- light
- ultraviolet
- pond body
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6482—Sample cells, cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
Abstract
The invention discloses a fluorescence and ultraviolet-visible absorption integrated flow cell. The fluorescence and ultraviolet-visible absorption integrated flow cell consists of a cell body and a cell base, wherein a liquid flow path is arranged in the cell body; a liquid inlet pipe and a liquid outlet pipe, which are communicated with the liquid flow path, are arranged on the cell body; a light inlet and a light outlet are formed in two ends of the liquid flow path respectively; a fluorescence window perpendicular to the light inlet and the light outlet is formed in the cell body; parts, expect the light inlet, the light outlet and the fluorescence window, of the cell body are blackened; a first optical fiber port, a second optical fiber port and a third optical fiber port are formed in the cell base and are opposite to the light inlet, the light outlet and the fluorescence window of the cell body respectively; a focusing lens is arranged between the fluorescence window and the third optical fiber port opposite to the fluorescence window, and is used for focusing light at the fluorescence window to the third optical fiber port. The fluorescence and ultraviolet-visible absorption integrated flow cell has the advantages of high sensitivity and small stray light, and is applied to the field of chromatographic analysis.
Description
Technical field
The invention belongs to chromatographic technique field, particularly, the present invention relates to the integrated flow cell of a kind of fluorescence and ultraviolet-ray visible absorbing.
Background technology
In efficient liquid phase chromatographic analysis, it is two kinds of the most frequently used detection meanss that fluorescence and ultraviolet-ray visible absorbing detect.At present, conventional liquid chromatographic detection adopts dedicated test pond, and user need to configure respectively independently fluorescence detector and ultraviolet-ray visible absorbing detecting device and detect for fluorescence and ultraviolet-ray visible absorbing, safeguards and use cost corresponding increasing all.For example China's national standard is to the aflatoxin fluorescence detection in milk, and melamine uv detection method causes relevant enterprise need to buy the detection that fluorescence and UV-detector are respectively used to aflatoxin and melamine simultaneously.If adopt fluorescence and ultraviolet-ray visible absorbing to detect the detecting device of one, can greatly save and safeguard and use cost.Because detection principle and the structure of fluorescence and ultraviolet-ray visible absorbing detection are not identical, be therefore difficult to by existing technology directly by different detection cell integrators.
There are at present the fluorescence of report and the integrated flow cell of ultraviolet-ray visible absorbing when can detecting ultraviolet-ray visible absorbing, mainly adopt 2 kinds of modes to collect fluorescence and detect.A kind of is the synchronous online flow cell that detects of a kind of absorbance as disclosed in Chinese patent 201010120953.3 and fluorophotometric, the mode that adopts fluorescence optical fiber interface holes right angle vertical with light source spectrum fiber interface hole to distribute, this mode can only detect the fluorescence of fluorescence optical fiber interface part, light to accept face less; The dual-purpose flow cell of another kind of ultraviolet-ray visible absorbing/fluorescent as disclosed in Chinese patent 200610009153.8, the light sending from light source one importing flow cell from bifilar bifurcation fiber, from another strand, derive fluorescence and detect, exist equally fluorescence to accept the problem that face is little.
Because China is more and more stricter to the requirement of the aspects such as food safety detection at present, lower to objectionable impurities detectability requirement wherein, as detecting, total aflatoxin content in liquid chromatography for national Specification-fluorescence detection mensuration food is limited to 1 μ g/kg, AFB in milk
1be 0.002 μ g/kg, AFB in milk powder
1be 0.02 μ g/kg.Therefore the sensitivity of fluorescence detector is required very highly, and a little less than the fluorescence of existing detecting device accepts the little meeting of face and cause fluorescent intensity that detecting device receives, be difficult to guarantee to meet the requirements of sensitivity.
Therefore, need a kind of highly sensitive, simple in structure portable fluorescence of exploitation and the dual-purpose flow cell of ultraviolet-ray visible absorbing badly.
Summary of the invention
The object of the invention is to, provide a kind of fluorescence and ultraviolet-ray visible absorbing integrated flow cell, this flow cell is simple and compact for structure, parasitic light is little, highly sensitive, can be used for fluorescence and ultraviolet-ray visible absorbing and detects.
For achieving the above object, the present invention has adopted following technical scheme:
The integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing, comprise pond body 1 and for the stall 2 of fixed spool body 1, in described pond body 1, be provided with liquid flow path 16, described pond body 1 is also provided with feed tube 11 and the drain pipe 12 being connected with liquid flow path 16, the two ends of described liquid flow path 16 are respectively light entrance 13, light outlet 14, described pond body 1 is also provided with and light entrance 13 and the perpendicular fluorescence window 15 of light outlet 14, and Darkening process is all done at the position of described pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15;
Position corresponding to pond body light entrance 13, light outlet 14 and fluorescence window 15 on described stall 2 arranges respectively the first optical fiber interface 21, the second optical fiber interface 22, the 3rd optical fiber interface 23, and between fluorescence window 15 and three optical fiber interface 23 corresponding with it, condenser lens 24 is installed, for the light of fluorescence window 15 being focused on to the 3rd optical fiber interface 23.
Preferably, described feed tube 11 and drain pipe 12 are arranged side by side, and those skilled in the art also can select other set-up modes as required.
Preferably, the making material of described pond body 1 is glass or quartz, and those skilled in the art also can select other to make material as required, such as organic glass (polymethylmethacrylate) etc.
The integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing is provided with fluorescence window 15, and by condenser lens 24, the fluorescence of fluorescence window 15 is assembled and carried out fluoroscopic examination, therefore fluorescence receptor area is larger, is provided with light outlet 14 simultaneously, for ultraviolet-ray visible absorbing, detects.
Darkening process is all done at the position of the integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15, avoided light in pond body 1, reflect and spread, form parasitic light, disturb fluorescence and ultraviolet-ray visible absorbing to detect.
The invention has the advantages that, the present invention has designed fluorescence and ultraviolet-ray visible absorbing light path on a pond body, increased fluorescence window, and fluorescence is focused on, non-light path part has been carried out to Darkening process simultaneously, therefore have highly sensitive, the advantage that parasitic light is little, be suitable for use in stratographic analysis field, be especially suitable for portable high-efficiency liquid chromatograph
Accompanying drawing explanation
Fig. 1 is the integrated flow cell schematic diagram of fluorescence of the present invention and ultraviolet-ray visible absorbing;
Fig. 2 is fluorescence of the present invention and the integrated flow cell of ultraviolet-ray visible absorbing pond body schematic diagram;
Fig. 3 is fluorescence of the present invention and the integrated flow cell stall of ultraviolet-ray visible absorbing schematic diagram;
Accompanying drawing sign:
1, pond body; 11, feed tube; 12, drain pipe; 13, light entrance; 14, light outlet; 15, fluorescence
Window; 16, liquid flow path; 2, stall; 21, the first optical fiber interface; 22, the second optical fiber interface; 23,
The 3rd optical fiber interface; 24, condenser lens.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described in detail.
As shown in Figure 1, the integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing, is provided with pond body 1 and for the stall 2 of fixed spool body 1.
As shown in Figure 2, in described pond body 1, be provided with liquid flow path 16, described pond body 1 is also provided with feed tube 11 and the drain pipe 12 being connected with liquid flow path 16, the two ends of described liquid flow path 16 are respectively light entrance 13, light outlet 14, described pond body 1 also arranges and light entrance 13 and the perpendicular fluorescence window 15 of light outlet 14, and Darkening process is all done at the position of described pond body 1 except light entrance 13, light outlet 14 and fluorescence window 15;
As shown in Figure 3, position corresponding to pond body light entrance 13, light outlet 14 and fluorescence window 15 on described stall 2 arranges respectively the first optical fiber interface 21, the second optical fiber interface 22, the 3rd optical fiber interface 23, and between fluorescence window 15 and three optical fiber interface 23 corresponding with it, condenser lens 24 is installed, for the light of fluorescence window 15 being focused on to the 3rd optical fiber interface 23.
Described feed tube 11 and drain pipe 12 are arranged side by side, and the making material of described pond body 1 is glass or quartz.
When the integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing carries out fluoroscopic examination, light by optical fiber guiding out light source, by the first optical fiber interface 21 of stall 2, enter the optical fiber entrance 13 of pond body 1, sample in liquid flow path 16 is excited, the fluorescence exciting is launched from fluorescence window 15, and after being focused on by condenser lens 24, enters the 3rd optical fiber interface 23, after deriving by optical fiber, by detecting device, carry out fluoroscopic examination.
When the integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing carries out ultraviolet-ray visible absorbing detection, light by optical fiber guiding out light source, by the first optical fiber interface 21 of stall 2, enter the optical fiber entrance 13 of pond body 1, by the absorption of sample part luminous energy in liquid flow path 16, from light outlet 14, penetrate, enter the second optical fiber interface 22, after deriving by optical fiber, by detecting device, carry out ultraviolet-ray visible absorbing detection.
The integrated flow cell of fluorescence of the present invention and ultraviolet-ray visible absorbing can carry out fluorescence simultaneously and ultraviolet-ray visible absorbing detects, light by optical fiber guiding out light source, by the first optical fiber interface 21 of stall 2, enter the optical fiber entrance 13 of pond body 1, sample in liquid flow path 16 is excited, the fluorescence exciting is launched from fluorescence window 15, and after being focused on by condenser lens 24, enters the 3rd optical fiber interface 23, after deriving by optical fiber, by detecting device, carry out fluoroscopic examination; Through the light of liquid flow path 16, by absorption of sample part luminous energy wherein, from light outlet 14, penetrate, enter the second optical fiber interface 22, after deriving by optical fiber, by detecting device, carry out ultraviolet-ray visible absorbing detection.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described.Although the present invention is had been described in detail with reference to embodiment, those of ordinary skill in the art is to be understood that, technical scheme of the present invention is modified or is equal to replacement, do not depart from the spirit and scope of technical solution of the present invention, it all should be encompassed in the middle of claim scope of the present invention.
Claims (3)
1. a fluorescence and the integrated flow cell of ultraviolet-ray visible absorbing, described flow cell comprises pond body (1) and for the stall (2) of fixed spool body (1), it is characterized in that, in described pond body (1), establish liquid flow path (16), on described pond body (1), be also provided with the feed tube (11) and the drain pipe (12) that are connected with liquid flow path (16), the two ends of described liquid flow path (16) are respectively light entrance (13) and light outlet (14), described pond body (1) is also provided with and light entrance (13) and the perpendicular fluorescence window (15) of light outlet (14), pond body (1) is except light entrance (13), Darkening process is all done at position outside light outlet (14) and fluorescence window (15),
The position of described stall (2) upper light entrance (13), light outlet (14) and fluorescence window (15) corresponding to pond body (1) arranges respectively the first optical fiber interface (21), the second optical fiber interface (22), the 3rd optical fiber interface (23), and between fluorescence window (15) and three optical fiber interface (23) corresponding with it, condenser lens (24) is installed, for the light of fluorescence window (15) being focused on to the 3rd optical fiber interface (23).
2. the integrated flow cell of fluorescence according to claim 1 and ultraviolet-ray visible absorbing, is characterized in that, described feed tube (11) and drain pipe (12) are arranged side by side.
3. the integrated flow cell of fluorescence according to claim 1 and ultraviolet-ray visible absorbing, is characterized in that, the making material of described pond body (1) is glass or quartz.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310701299.9A CN103698276B (en) | 2013-12-18 | 2013-12-18 | A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell |
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| CN201310701299.9A CN103698276B (en) | 2013-12-18 | 2013-12-18 | A kind of fluorescence and ultraviolet-ray visible absorbing integration flow cell |
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| CN103698276A true CN103698276A (en) | 2014-04-02 |
| CN103698276B CN103698276B (en) | 2016-01-20 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969187A (en) * | 2014-05-07 | 2014-08-06 | 大连理工大学 | Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection |
| US9518923B1 (en) | 2015-12-07 | 2016-12-13 | International Business Machines Corporation | System and methods for fluorescence detection |
| CN109932450A (en) * | 2019-04-03 | 2019-06-25 | 安徽皖仪科技股份有限公司 | Flow cell and liquid chromatograph with it |
| CN110006859A (en) * | 2019-03-05 | 2019-07-12 | 北京雪迪龙科技股份有限公司 | A kind of fluorescent material detection device and the method for detecting fluorescent material |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103969187A (en) * | 2014-05-07 | 2014-08-06 | 大连理工大学 | Fluorescence spectrophotometer flow cell assembly for fluorescent online quantitative detection |
| US9518923B1 (en) | 2015-12-07 | 2016-12-13 | International Business Machines Corporation | System and methods for fluorescence detection |
| CN110006859A (en) * | 2019-03-05 | 2019-07-12 | 北京雪迪龙科技股份有限公司 | A kind of fluorescent material detection device and the method for detecting fluorescent material |
| CN109932450A (en) * | 2019-04-03 | 2019-06-25 | 安徽皖仪科技股份有限公司 | Flow cell and liquid chromatograph with it |
| US11378559B2 (en) | 2019-04-03 | 2022-07-05 | Anhui Wayee Science and Technology Co., Ltd. | Flow cell and liquid chromatographic unit having same |
| CN111650123A (en) * | 2020-06-22 | 2020-09-11 | 广东省测试分析研究所（中国广州分析测试中心） | Aflatoxin in-situ derived fluorescence detection device |
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| Publication number | Publication date |
|---|---|
| CN103698276B (en) | 2016-01-20 |
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