CN105510371A - Low-field nuclear magnetic resonance-based detection method of fat content for living animals - Google Patents
Low-field nuclear magnetic resonance-based detection method of fat content for living animals Download PDFInfo
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- CN105510371A CN105510371A CN201510859303.3A CN201510859303A CN105510371A CN 105510371 A CN105510371 A CN 105510371A CN 201510859303 A CN201510859303 A CN 201510859303A CN 105510371 A CN105510371 A CN 105510371A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
Abstract
The invention discloses a low-field nuclear magnetic resonance-based detection method of fat content for living animals. The method comprises the following steps: separately collecting nuclear magnetic resonance signals of lean meat and water in a living animal to be measured by using an IR sequence, bringing the nuclear magnetic resonance signal of lean meat in the living animal to be measured in a working curve 1 of chicken, and calculating mass of the lean meat; bringing the nuclear magnetic resonance signal of water in the living animal to be measured in a working curve 1 of water, and calculating mass of water; collecting a resultant magnetic signal of the living animal to be measured by using a CPMG sequence; bring the obtained mass of lean meat and the obtained mass of water in a working curve 2 of chicken and a working curve 2 of water respectively, in order to obtain CPMG sequence nuclear magnetic resonance signals corresponding to lean meat and water, subtracting the CPMG sequence nuclear magnetic resonance signals corresponding to lean meat and water from the resultant magnetic signal of the living animal to be measured, and bringing the value in a working curve of edible oil, in order to obtain the mass of fat. The method has the advantages of non-destructive rapid determination of fat content for living animals without pretreatment process, and low usage cost.
Description
Technical field
the invention belongs to low-field nuclear magnetic resonance technical field, relate to a kind of method detecting living animal fat content based on low-field nuclear magnetic resonance.
Background technology
method for detecting living animal fat content in prior art mainly contains CCA(whole body chemical composition analytic approach), BIA(Bioelectrical impedance analysis) and DXA(Dual energy X ray absorptiometry), its concrete shortcoming is as follows:
cCA(whole body chemical composition analytic approach): testing result is accurate, but needs to use chemical reagent to carry out extracting fat, consuming time, need put to death animal;
bIA(Bioelectrical impedance analysis): be a kind of technology being measured body water by electrical method.Principle is that when faint ac current signal is imported human body, electric current can be little along with resistance, the good body fluid motion of conductive performance.The number of moisture decides the electric conductivity of current path, and this can represent by the measured value being called impedance.The General Principle calculating human body constituent to measure impedance utilizes body water to be directly proportional to height, is inversely proportional to human body impedance.Need anesthesia, error is larger;
dXA(Dual energy X ray absorptiometry): the X ray that X ray bulb sends is after K-edge filtering, form the peak that 70KeV with 38KeV two energy are different, they have different attenuation rate when the different bone of density, lean tissue mass (LTM), adipose tissue (FTM).Need anesthesia, length consuming time (5-35min), has Radiation On Human body animal pest.
Summary of the invention
the present invention seeks to: provide a kind of can the fat content of Fast Measurement living animal, use cost is low, and without any need for the method detecting living animal fat content based on low-field nuclear magnetic resonance of pretreatment process.
technical scheme of the present invention is: a kind of method detecting living animal fat content based on low-field nuclear magnetic resonance, specifically comprises the steps:
step 1) takes chicken, water, the edible oil making standard specimen of multiple quality, uses IR sequence to gather the nuclear magnetic signal of chicken, water in chicken, water, edible oil mixed sample respectively; Use the quality of chicken as ordinate, the nuclear magnetic signal of chicken, as horizontal ordinate, is drawn scatter diagram, is set up chicken working curve one; Use the quality of water as ordinate, the nuclear magnetic signal of water, as horizontal ordinate, is drawn scatter diagram, is set up water working curve one;
step 2) use cpmg sequence row to gather the nuclear magnetic signal of chicken, water, edible oil respectively; Use the quality of chicken as ordinate, the nuclear magnetic signal of chicken, as horizontal ordinate, is drawn scatter diagram, is set up chicken working curve two; Use the quality of water as ordinate, the nuclear magnetic signal of water, as horizontal ordinate, is drawn scatter diagram, is set up water working curve two; Use the quality of edible oil as ordinate, the nuclear magnetic signal of edible oil, as horizontal ordinate, is drawn scatter diagram, is set up edible oil working curve;
step 3) uses IR sequence to gather the nuclear magnetic signal of lean meat, water in living animal to be measured respectively, brings the lean meat nuclear magnetic signal of living animal to be measured into above-mentioned chicken working curve one, calculates lean mass; Bring the water nuclear magnetic signal of living animal to be measured into above-mentioned water working curve one, calculate quality;
step 4) uses cpmg sequence row to gather total nuclear magnetic signal of living animal to be measured; Lean mass step 3) obtained, quality substitute into chicken working curve two, water working curve two respectively, obtain lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding, total nuclear magnetic signal of living animal to be measured is adopted to deduct lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding, then edible oil working curve is substituted into, obtain fat mass, finally obtain its fat content in conjunction with living animal gross mass to be measured.
principle of work of the present invention: nuclear magnetic resonance is by H proton resonance in excited sample, and produce nuclear magnetic signal, nuclear magnetic signal intensity changes along with the difference of die-away time.The amount of nuclear magnetic signal intensity and sample H proton is linear.What can produce nuclear magnetic signal in animal body mainly contains fat, lean meat, water.But the nuclear magnetic relaxation times due to these three kinds of materials has very large otherness, nuclear-magnetism technology can be utilized to distinguish these three kinds of materials, and carry out quantitative test.
advantage of the present invention is:
1. the present invention is compared with existing living animal fat content detection method, it is a kind of direct measuring method, its test speed fast (1-2 minute), testing result precision is high, adopt low-field nuclear magnetic resonance equipment, use low cost, also without any need for pretreatment process, obtain living animal fat content in the situation of not anaesthetizing simultaneously, more safe and reliable.
Accompanying drawing explanation
below in conjunction with drawings and Examples, the invention will be further described:
fig. 1 is IR sequence diagram of the present invention;
fig. 2 is that cpmg sequence of the present invention lists intention;
fig. 3 is the mouse nuclear-magnetism method measurement result of different fat content and the linear analysis figure of smart standard whole body chemical composition analytic approach.
Embodiment
embodiment:
one, test objective
obtain mouse adipose content by the inventive method, and contrast with the fat content that the measurement of chemical composition analysis method obtains.
two, test material
mouse 26.
three, test apparatus
miniQMR-60 toy body composition analysis of regaining consciousness becomes to produce with imaging system Suzhou Niumai Electronics Co., Ltd., and resonant frequency 23.346MHz, magnet strength 0.54T, probe coil diameter 70mm, experimental temperature controls at 31.99 ~ 32.01 DEG C.
four, the preparation of test specimen
take experiment mice weight, mouse is put into dress mouse clamper.
five, process of the test
1. adopt IR sequence acquisition mouse nuclear magnetic signal, as Fig. 1, (in Fig. 1, RF is equipment radio frequency pulse schematic diagram, and Rx is collection signal figure, and first echo binds a little as signal value.Because lean meat and the water T2 relaxation time variant, the lean meat relaxation time is short, the water relaxation time is long, if shown first echoed signal is the resultant signal of lean meat and water, echoed signal after decay 1000ms is the signal of water) shown in, obtain lean meat, water signal, bring the lean meat nuclear magnetic signal of mouse into chicken working curve one, calculate lean mass; Bring the water nuclear magnetic signal of mouse into water working curve one, calculate quality;
2. adopt cpmg sequence row to gather mouse nuclear magnetic signal, as shown in Fig. 2 (in Fig. 2, RF is equipment radio frequency pulse schematic diagram, and Rx is collection signal figure, and first echo binds a little as signal value), obtain the total nuclear magnetic signal of mouse;
3. lean mass obtained above, quality are substituted into chicken working curve two, water working curve two respectively, obtain lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding;
4. adopt mouse total nuclear magnetic signal to deduct lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding, then substitute into edible oil working curve, obtain fat mass, finally obtain its fat content in conjunction with mouse quality;
5. by sacrifice, take out adipose tissue, use ether to carry out extracting fat, weigh and obtain chemical composition analysis method fat content;
6., as shown in Figure 3, with chemical composition analysis method fat content for horizontal ordinate, low field nuclear-magnetism method fat content is ordinate, fitting a straight line, and the linearity 0.997, slope 0.996, illustrates the measurement result of two kinds of its fat contents of method closely.
above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art scholar all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as have in art usually know the knowledgeable do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (1)
1. detect a method for living animal fat content based on low-field nuclear magnetic resonance, it is characterized in that, specifically comprise the steps:
Step 1) takes chicken, water, the edible oil making standard specimen of multiple quality, uses IR sequence to gather the nuclear magnetic signal of chicken, water in chicken, water, edible oil mixed sample respectively; Use the quality of chicken as ordinate, the nuclear magnetic signal of chicken, as horizontal ordinate, is drawn scatter diagram, is set up chicken working curve one; Use the quality of water as ordinate, the nuclear magnetic signal of water, as horizontal ordinate, is drawn scatter diagram, is set up water working curve one;
Step 2) use cpmg sequence row to gather the nuclear magnetic signal of chicken, water, edible oil respectively; Use the quality of chicken as ordinate, the nuclear magnetic signal of chicken, as horizontal ordinate, is drawn scatter diagram, is set up chicken working curve two; Use the quality of water as ordinate, the nuclear magnetic signal of water, as horizontal ordinate, is drawn scatter diagram, is set up water working curve two; Use the quality of edible oil as ordinate, the nuclear magnetic signal of edible oil, as horizontal ordinate, is drawn scatter diagram, is set up edible oil working curve;
Step 3) uses IR sequence to gather the nuclear magnetic signal of lean meat, water in living animal to be measured respectively, brings the lean meat nuclear magnetic signal of living animal to be measured into above-mentioned chicken working curve one, calculates lean mass; Bring the water nuclear magnetic signal of living animal to be measured into above-mentioned water working curve one, calculate quality;
Step 4) uses cpmg sequence row to gather total nuclear magnetic signal of living animal to be measured; Lean mass step 3) obtained, quality substitute into chicken working curve two, water working curve two respectively, obtain lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding, total nuclear magnetic signal of living animal to be measured is adopted to deduct lean meat, cpmg sequence row nuclear magnetic signal that water is corresponding, then edible oil working curve is substituted into, obtain fat mass, finally obtain its fat content in conjunction with living animal gross mass to be measured.
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Cited By (7)
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| CN106290442A (en) * | 2016-09-22 | 2017-01-04 | 大连工业大学 | Utilize the method for body component content in low-field nuclear magnetic resonance technology for detection mice |
| CN107525820A (en) * | 2017-09-30 | 2017-12-29 | 上海纽迈电子科技有限公司 | A kind of method based on low-field nuclear magnetic resonance measure Mice Body composition |
| CN112285144A (en) * | 2020-10-15 | 2021-01-29 | 青岛农业大学 | Method for detecting breast myopathy of white feather broiler chicken by using low-field nuclear magnetic resonance |
| CN112683940A (en) * | 2020-12-24 | 2021-04-20 | 恒天海龙（潍坊）新材料有限责任公司 | Method for rapidly determining content of flame retardant in inorganic flame-retardant cellulose fiber |
| CN112946003A (en) * | 2019-12-11 | 2021-06-11 | 上海纽迈电子科技有限公司 | Method for detecting fat content in milk powder |
| CN113125488A (en) * | 2021-04-21 | 2021-07-16 | 南京农业大学 | Method for quickly identifying fat-filled artificial snowflake beef |
| CN114460121A (en) * | 2022-01-28 | 2022-05-10 | 中国农业科学院农业质量标准与检测技术研究所 | Method for detecting water content and fat content of livestock meat by using low-field nuclear magnetic resonance technology |
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| CN107525820A (en) * | 2017-09-30 | 2017-12-29 | 上海纽迈电子科技有限公司 | A kind of method based on low-field nuclear magnetic resonance measure Mice Body composition |
| CN112946003A (en) * | 2019-12-11 | 2021-06-11 | 上海纽迈电子科技有限公司 | Method for detecting fat content in milk powder |
| CN112285144A (en) * | 2020-10-15 | 2021-01-29 | 青岛农业大学 | Method for detecting breast myopathy of white feather broiler chicken by using low-field nuclear magnetic resonance |
| CN112683940A (en) * | 2020-12-24 | 2021-04-20 | 恒天海龙（潍坊）新材料有限责任公司 | Method for rapidly determining content of flame retardant in inorganic flame-retardant cellulose fiber |
| CN112683940B (en) * | 2020-12-24 | 2022-07-19 | 潍坊欣龙生物材料有限公司 | Method for rapidly determining content of flame retardant in inorganic flame-retardant cellulose fiber |
| CN113125488A (en) * | 2021-04-21 | 2021-07-16 | 南京农业大学 | Method for quickly identifying fat-filled artificial snowflake beef |
| CN114460121A (en) * | 2022-01-28 | 2022-05-10 | 中国农业科学院农业质量标准与检测技术研究所 | Method for detecting water content and fat content of livestock meat by using low-field nuclear magnetic resonance technology |
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