CN1216255A - Preparation of ethanol extracted earthworm tissue liquid - Google Patents
Preparation of ethanol extracted earthworm tissue liquid Download PDFInfo
- Publication number
- CN1216255A CN1216255A CN98118193A CN98118193A CN1216255A CN 1216255 A CN1216255 A CN 1216255A CN 98118193 A CN98118193 A CN 98118193A CN 98118193 A CN98118193 A CN 98118193A CN 1216255 A CN1216255 A CN 1216255A
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- Prior art keywords
- ethanol
- preparation
- tissue liquid
- earthworm
- earthworm tissue
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
Earthworm paste prepared through washing, soaking and crushing is added into ethanol and through reflux and concentration process, earthworm tissue liquid up to the pharmaceutical standard is obtained. Earthworm tissue liquid is used in medicine for preventing and curing cancer, thrombolytic medicine, hypertension medicine, etc.
Description
The present invention relates to the preparation method of earthworm tissue liquid extractive technique, particularly ethanol extracted earthworm tissue liquid.
Lumbricus common name Pheretima belongs to chaetopoda, Oligotrichida.The Main Ingredients and Appearance of Lumbricus is to separate thermokalite, xanthine, adenine, choline and multiple human body necessary aminoacid and trace element.According to the Compendium of Material Medica record of China's " pharmacopeia complete works " and Li Shizhen (1518-1593 A.D.), Lumbricus " has heat clearing away, suppressing the hyperactive liver, Zhichuan, leads to effects such as whole.Chinese patent CN1087817 discloses a kind of " earthworm component extracting process ", and this method is the self-dissolving by Lumbricus, obtains the different extract of several molecular weight with the method for ultrafiltration, is applicable to different purposes respectively.But this complex process, equipment is many, and investment is big, is not suitable for general small processing factory.
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art, provide a kind of preparation technology simple, easy to operate, small investment, the preparation method of the ethanol extracted earthworm tissue liquid of instant effect.
The object of the present invention is achieved like this: a kind of preparation method of ethanol extracted earthworm tissue liquid is characterized in that: get Lumbricus clear water washing alive, soaked 24 hours, drain after pulling out, take by weighing 2 parts of weight, be ground into pasty state, add 6.5 parts of weight of ethanol, reflux twice, each 2 hours, slagging-off after filtration, reclaim ethanol, concentrated filtrate is 1 part of weight, and to transfer its pH value be 6.8-7.5, static filtration, the bottling sterilization gets product; Used ethanol is the ethanol of 70-95%; Regulate pH value with 10% sodium hydroxide.
Technical process of the present invention is simple, be convenient to implement, and product just can reach the medical and health standard through disposable production.
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
Get anosis nontoxic Lumbricus alive, behind the clear water thorough washing, soaked 24 hours, pull out and drain, weigh 100 kilograms, be ground into pasty state, add 325 kilograms of 70% ethanol, reflux twice, each 2 hours, after filtration after the slagging-off, reclaim ethanol, filtrate is concentrated into 50 kilograms, transferring pH value with 10% sodium hydroxide is 6.8, static filtration, the bottling sterilization gets product.Add the desired concentration of distillation water-dilutable.
Embodiment 2:
Get anosis nontoxic Lumbricus alive, behind the clear water thorough washing, soaked 24 hours, pull out and drain, weigh 200 kilograms, be ground into pasty state, add 650 kilograms of 95% ethanol, reflux twice, each 2 hours, after filtration after the slagging-off, reclaim ethanol, filtrate is concentrated into 100 kilograms, transferring pH value with 10% sodium hydroxide is 7.5, static filtration, the bottling sterilization gets product.Add the desired concentration of distillation water-dilutable.
Claims (3)
1. the preparation method of an ethanol extracted earthworm tissue liquid is characterized in that: get Lumbricus clear water washing alive, soaked 24 hours, drain after pulling out, take by weighing 2 parts of weight, be ground into pasty state, add 6.5 parts of weight of ethanol, reflux twice, each 2 hours, slagging-off after filtration, reclaim ethanol, concentrated filtrate is 1 part of weight, and to transfer its pH value be 6.8-7.5, static filtration, the bottling sterilization gets product.
2. the preparation method of earthworm tissue liquid according to claim 1 is characterized in that used ethanol is the ethanol of 70-95%.
3. the preparation method of earthworm tissue liquid according to claim 1 is characterized in that regulating pH value with 10% sodium hydroxide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98118193A CN1063637C (en) | 1998-09-30 | 1998-09-30 | Preparation of ethanol extracted earthworm tissue liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98118193A CN1063637C (en) | 1998-09-30 | 1998-09-30 | Preparation of ethanol extracted earthworm tissue liquid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1216255A true CN1216255A (en) | 1999-05-12 |
| CN1063637C CN1063637C (en) | 2001-03-28 |
Family
ID=5225957
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98118193A Expired - Fee Related CN1063637C (en) | 1998-09-30 | 1998-09-30 | Preparation of ethanol extracted earthworm tissue liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1063637C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009014411A1 (en) * | 2007-07-23 | 2009-01-29 | Gutierrez Gaitan Rogelio Salva | Drug that can reduce cholesterol and urea levels and stabilise glucose, creatinine and triglyceride levels, prepared from eisenia foetida |
| CN101829148B (en) * | 2009-03-10 | 2011-09-07 | 李振国 | Traditional Chinese medicine extractive, oral preparation containing extractive and method for preparing oral preparation |
| US8145660B2 (en) | 2007-10-05 | 2012-03-27 | Fujitsu Limited | Implementing an expanded search and providing expanded search results |
| CN105412154A (en) * | 2015-12-02 | 2016-03-23 | 云南永安制药有限公司 | Preparing method for fresh lumbricus extract |
-
1998
- 1998-09-30 CN CN98118193A patent/CN1063637C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009014411A1 (en) * | 2007-07-23 | 2009-01-29 | Gutierrez Gaitan Rogelio Salva | Drug that can reduce cholesterol and urea levels and stabilise glucose, creatinine and triglyceride levels, prepared from eisenia foetida |
| US8145660B2 (en) | 2007-10-05 | 2012-03-27 | Fujitsu Limited | Implementing an expanded search and providing expanded search results |
| CN101829148B (en) * | 2009-03-10 | 2011-09-07 | 李振国 | Traditional Chinese medicine extractive, oral preparation containing extractive and method for preparing oral preparation |
| CN105412154A (en) * | 2015-12-02 | 2016-03-23 | 云南永安制药有限公司 | Preparing method for fresh lumbricus extract |
| CN105412154B (en) * | 2015-12-02 | 2018-11-09 | 云南永安制药有限公司 | A kind of preparation method of fresh Pheretima extract |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1063637C (en) | 2001-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20010328 Termination date: 20100930 |