DE2451769A1 - METHOD AND DEVICE FOR AUTOMATIC READING AND DETERMINATION OF REACTION RESULTS - Google Patents

Description

Kamiiurg ,. October 30, 1974

Osmo Antero Suovaniemi ZhO \ / b 9

Armas Lindgrenintie 15 A

00570 Helsinki 57 / Finland Lawyer File; 3461

Method and device for automatic reading and St-StiKiraen of reaction results.

The invention relates to a method and an apparatus to use automatic reading and registration from results of many virological, bacteriological and similar urn., toi ο gis rather reactions.

In addition, the device can use spectral ο photometric methods, e.g. used when measuring and registering color reactions.

In laboratories it is necessary to search for pathogens or when assessing the content of the various substances in the organs of the body, numerous reaction studies are often carried out will. In these exams you deal with hundreds, yes thousands of test tubes in which e.g. dilution series or chemical color reactions are carried out.

For virological disease determinations or diagnoses can be achieved by separating the virus itself or its parts and visualizing them in the electron microscope reach. In addition, a diagnosis is often made when the increase the opposite titer of the virus in question in the blood

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ORIGINAL

or the virus antigens are known in the cells.

In determining the basic virological procedure, which If pathogens are in the patient's sample, dilution and titration are central to many processes of the samples, many reactions are used in the investigation process, the results of which are visualized from the dilution series can be read. These reactions are u, a, haema, ggluti nation, the binding of complements and the inhibition of hemagglutination

It is often the case that diluting for each sample many times must be repeated one after the other, and besides that Control titration to improve reliability should be done often. Often thousands of Sample tubes and the results are read visually. Reading the results is mainly done with current methods primitive. Such reading the results and determining goes slowly, of course, and there are numerous possibilities for mistakes.

Efforts are made in some of the bacteriological diagnoses Respect as with the virological.

In many bacteriological test procedures one uses dilution series, and the results are read visually. Common procedures of this type include those for hemolytic response based determination of the anti-streptolysis titer of streptococci (AST) and the titration of staphylococci (ASTA). The Widalsehe method clarifies the bacterial agglutination based reaction, which can happen in salmonella or in samples of the typhoid type. ■

In hematology, especially when determining blood groups

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if there are many samples, one often has with grossea. Do lots of mohawks, and the results will be visual

read. Numerous trade fairs are held every day in the lu.borato.rien made with the spectrophotometer. devices available in the market automatically. The measurements . u: id results are primitive. In some of these devices one automatically messe'jV and the events e.g. from one to judge a hundred samples. In these commercially available spectrophotometers, the light is perpendicular through the walls of the Cuvette, - whereby the light e.g. does not reach the mm bottom of the Cuvette located precipitation can hit.

In the device according to the invention, the light is transmitted through the in the direction of the longitudinal axis of the cuvette running liquid column located in the cuvette, and then through the delimiting Bottom of the cuvette or in the opposite direction. If one considers the liquid space in the cuvette changes, one changes at the same time the path of the light running through the liquid, which also changes the transparency or The transmittance of the liquid column is changed. If the light goes in the direction of the longitudinal axis of the cuvette, the precipitate can occur serological, bacteriological or hematological Reactions are true, and because of the change in the presented ones. Light intensity can be measured. Precipitation or cloudiness can be measured with commercially available spectrophotometers do not measure. For example, hemolysis causes hemolysis reactions a clarification of the whole mix of actions, where-. which in turn increases the light transmission. Even a certain degree of hemolysis or result point from the Change of the specified light intensity can be determined.

The method according to the invention is mainly thereby ge

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that when measuring the © uvette, the cuvette row or the cuvette group, light is passed through the column of liquid to be read or measured in each cuvette (C, 10) and through the delimiting cuvette base into the reading device (^ 2.8) " is that, runs through each cuvette (^ 10-) in the direction of the longitudinal axis of the cuvette (G-, Ίθ) from the light source located on one side of the cuvette to the detector (3> ~ 14) located on the other side of the cuvette, with the light running in the direction of the longitudinal axis of the cuvette, the results of the serological, bacteriological and hematological reactions or the color reaction on the cuvette or cuvettes in place can be read from the & E &-iH; on the bottom or not in the liquid column decreased precipitation, cloudiness, etc. or from the changes in the color of the liquid in the cuvette or cuvettes, the change in light transmission ity caused by the change in the specified intensity of the light.

The device according to the invention is mainly characterized in that that the device comprises the cuvettariums fitted into a cuvettarium frame, their fixed or detachable adapted ones Cuvettes in the position to one another as the detectors located in the boiler device and the light sources corresponding to them are attached, and that the measuring window of the cuvettes is suitable is surrounded on the outside by a cylindrical protective part or edge,

In some of the commercially available cuvettes in which the Measuring through the "% nde of the cuvette is done horizontally, the

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Walls of the cuvette slightly scratched and move "when touched with your fingers, which leads to big mistakes in lües & en can. Furthermore, with the devices now on the market, where the light runs horizontally, the straightening of the

is. Ouvettes have not been given enough attention, and often

this circumstance has been completely ignored and safeguarded.

In laboratories, the production of reaction mixtures and their measurement in the photometer is a daily work routine. The reaction mixtures obtained by pipetting the oil sample and one or more reagents are used to measure, among other things, the content of certain substances in the sample or the kinetics of the enzymes in the photometer. With such measurements, errors can arise in the end result, resulting from errors either in the used devices or from errors in use. Ernest Kaolin and his workmates have shown (Clinical Chemistry 19/8, 832-837, 1973) - that in the case of kinetic measurements of enzymes the errors in the end result can in the worst case be around - 40 $. These errors are caused by devices, inaccuracies in the length of the light path, the temperature, the pipetting of the samples and reagents, the time and the optical and electronic parts of the photometer.

Nowadays, efforts are made to produce the reaction mixture belt to keep the sample and reagent quantities as small as possible. A so-called micro-process has been used, whereby man JRe.agenzunkO3ten saved, and from one sometimes a lot Difficult, available sample, you can make several beatings.

The volumes pipetted using the microprocessing are small, whereby the percentage error in plating can be very large percentage, which in turn 'is an incorrect end result

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caused. Likewise, "in photometry ache η jiiossen, in which the reaction mixtures are produced by micro-processes, the faulty portion in the final boiler result may be increased.

The invention is based on the following description and the attached .Drawings show in which Pig. 1 and 2 schematically examples of the reading device according to the invention demonstrate,

Pig. 3a and 3b show two examples of the result cards used in the results part of the reading device, which are accessed by the reading device the results as well as the name of the patient is written.

Pig. 4 shows, viewed from the side, the one in the cuvettarium frame. fitted cuvettaria in measurement position.

Pig. 5 shows the cuvettarium fitted into the cuvettarium frame seen from above,

Pig. ' 6 shows the guvettarium from the side, Pig. 7 shows the cover plate of the Cuvettarium from the side, Pig, 8 shows the cuvettarium from above, Pig. 9-shows a single cuvette from the side and in section, Pig. 10 shows the cuvettarium and the cuvettes belonging to it for Measure; directed,

Pig. 11 shows the reagent block in section and from the side, Pig. 12 shows the cuvettarium and the tips used in it of Yielstep serial pipettes in section and from the side, Pig. 13 shows Pig's Cuvettarium. 2 and the top of the Serial pipette in the phase in which the liquid container of the serial pipette is filled,

Pig. 14 shows the reagent block seen from above, and Pig. 15 shows from the side and in section that for the photo-

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metric Meseenping according to the invention «and. with the lid provided Cuvetturium.

The titration or other reactions are done in the cuvette, those in the frame symmetrically, at certain distances from one another are attached in several rows. Such a frame is built to match the reading device. In the one shown in Fig.l Embodiment are in the support 1 at a corresponding distance from the cuvettes C, the light source 2 (II, L2, I3) and the Detectors 3 (Dl, D2, D3) attached. Light sources 2 and Detek-. gates 3 can be built for the reading device, e.g. as many as Cuvette rows are present. In this case the column ί automatically movable on the rows of cuvettes, from one cuvette to the other made, or the cuvette frame is movable and support 1 stays in place. The device can of course also be built in such a way that a light source and a corresponding detector is available, whereby there are no mechanically moved components in the device. '/

If the light runs e.g. from the light source Ll and over the cuvette Cl in the direction of the longitudinal axis of the cuvette, it touches Light first the liquid surface, runs <4, nn through the Column of liquid ^ through the bottom of the cuvette to that of the cuvette Cl corresponding detector Dl, in this case the knife registers 4-die preaised light intensity. The reading device measures every cuvette in the cuvette row. With any of the cuvettes in the Cuvette series there is a definitely large change in the light intensity that is unique to each reaction, which is caused by dea on the floor the cuvette located sediment, from the Truebung oäer the Light transmission of the liquid in the curette takes place, and this shows the desired change point in the dilution series, and from the result can be read off in order to

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which cuvette of the cuvette series it is, ¹ ·

In the result part 5 one uses. either consistent logic or acceptance of the Dator results, to remember and preserve the program that calculates the result in the desired foisa. When registering, one can use the Lrgebaiskarteη shown in Fig. 3, in which the reaction is indicated by the directory β and the change in light intensity corresponding to this reaction with the corresponding code «Another possibility is that _? that in the feedback part there are regulators ₉ which are set to the difference and the standard of the light intensity that are specific to each reaction, 5 are set in the corresponding positions ₉ and in the results part is written afterwards ifamen and result; · "of the reaction in the result card, as in Hg . 3b is displayed «, -

The reading device can also be used as a spectrophotometer, when the meter registers the amount of light coming to the corresponding detector from each light source and the result part registers the absorbance and / or the transmittance of the solution of each cuvette.

In FIG. Z , another exemplary embodiment of the reading device is shown schematically, in which a uniform light source 8 is used and the light is passed through fiber optics 9 through Cuvette IO, from where it continues to fiber optics 11. From here the light continues and runs around the perimeter in the fiber optics.These fibers coming to the perimeter are read one after the other at the reading point 12, and the fiber optic 13 pushes the light on to the detector 14.

The device according to the invention comprises the essential part Cuvetturium. In the cover plate 17 of the Cuvettarium 16 are several cuvettes 18 located in a certain space between them. These serve to measure the absorbance of the

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ITeU'sigkeii in the cuvette 18 ":, indent de: · 'Precipitation on the bottom of the cuvette 18" or the cloudiness causing a change in the light intensity «, With the Yertikalmess-2: i'iii2, ip the light passes through the bottom of the cuvette 18 and the light intensity is registered on the detector 19 located on the top of the cuvette.

Jim'm 'Cuvette] Π de fs Cuvettariums 16 can simultaneously through the Dual: el disk 20 GESEkiKsaKHXsrsKasH for storing the cuvettarium 16 or closed during shaking. The cuvettes 18 de3 Cuvettariums 16 are directed so that in them the vertical measuring principle can be carried out «,

The measuring window 21 of the cuvette 18 is from the outside of a cylinder-like part or edge 22 surrounding the measuring window vox * Schilt tern and 3c bend protects, whereby the measuring window 21 of the cuvettes 18 of the cuvettarium 16 are optically at a high level. If the bottom of the cuvette is concave on the inside, the precipitate in the cuvette usually settles in the form of a "button" In the middle of the bottom of the cuvette. If the bottom of the cuvette is on the inside is concave. and on the outside, the light path looks like a plano-concave lens. This is easy to do Produce cuvettes whose optical properties are the same. if If the device is used like a spectrophotometer, the bottom of the cuvette can also be flat, with the light path not being the Effect of a lens.

the base 21 of the cuvette 18 of the cuvette 16 is flat, the cuvette base is easy to manufacture, and there are no optical effects error, also no lens effect in the light path. Still can the bottom and / or lid of the ouvette of the Cuvettarium is flat, concave od; r be convex, n.xh inside or outside depending on the purpose and to achieve the right lens effect. . BATH ORIGINAL

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The lower part of the Cuvetteri 18 of the Cuvettardun-s 16 is solidly conical, through which the guvettes of the Cuvetturium can be easily directed (sI ¹ Ig ₈ 12), and the deposit particles from the liquid layer from the upper part of the Cuvette are on the bottom of the Kegels 23 set as buttons as buttons. The measurement of the sediment and the turbidity in a small area is easy.

Aa of the vertical inner wall of the cuvette 18 can have one or more Cassettes located in the seal of the longitudinal axis are attached the fluid mixture in the cuvette 4 promote when moving the cuvette. Through excentromisehendes ! shake those in the rotating cuvette η " Columns of liquid in circular motion, the columns of liquid rise up along the wall of the cuvette. Here the different layers of liquid mix poorly. From tr when unc't the walls the cassettes are 24-, as shown above, cause these cassettes in the orbiting liquid column . Vortex, whereby the different layers of liquid are mixed effectively and even with force.

Also of importance is the fact that the cuvettarium 16 is only in one position. the Cuvettarium frame 15 can be used can. In the Cuvettarium frame 15 is a projection 25 for the In the cover plate 17 located incision or the opening 26 fits. In the cover plate 17 of the Cuvettarium 16 is also a Code.27, from which one of the measuring device and / or visually can examine the cuvettarium 16 in question and at the same time each sample in the cuvettes 18 of the cuvettarium 16. This has the advantage that with just one code 27 nine different Can examine samples and avoid the code character for each sample can,-

BATH ORIGINAL

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Since the Cuvettarium 16 is only placed in one position in the Cuvettarium 1! 5 stelleLi in which the measurement of the cuvettes 18 of the üuvettariums 16 is going on in the measuring device, one can Set nine channels to zero in the measuring device, and in the following ones Cuvuttariums measure the corresponding canals. From that one draws the The advantage is that if n small optical errors occur on the exhibition window 21 of the cuvette and the errors are reflected in every cuvettarium repeat, the fault is eliminated in the zero phase and then in the following measurement phases.

The Cuvett irium frame 15 is planned in such a way that one or more Cuvettaria 16 can be placed, and they can be pushed through the measuring end 'of the measuring device (is. Fig. 4)

The cuvettarium frame 15 functions as a frame for storage and conveying the cuvettes in dosing liquids into and from the guvettaria, during incubations: i.and measuring the reactions.

The Cuvettarium frame 15 can also be heated, whereby the Cuvettes 18 of the cuvettaria 16 and the liquids contained therein receive the desired temperature.

i n the /

When the cuvettarium 16 / cuvettarium rack 15 located at the measuring end of the measuring device is at the measuring point, each cuvette of the cuvettarium is protected against outside light, and the cuvettarium rack also provides each cuvettarium with light protection from the other cuvettariums.

If at the end of the measuring device the in the Cuvettariumge alternate If the Cuvettarium 16 is located, it comes to the measuring point, the light beam penetrates through the opening 28 in the Cuvetturium frame 15, and this light beam between 29-29 'gives the detector 29' the pulse or through the mediation of light dior to the straightening part 30 of the measuring end.

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This Riclitteil 30 directed each cuvette 18 ces Cuvettariums 16 simultaneously to the square of the corresponding cuvettes 18 and detectors 19 lichtfibe'rn .Venn the increased fiiohtteil 30 production in the (/, each cuvette is of Cuvett ^ Ministry Liit ihrera lower part 23 in The cone-like part of the straightening device is set and, in addition, the cuvettarium has risen to the measuring end, with the upper part of the cuvetturium 16 supporting the body of the measuring end of the detector end. In this position, the measurement takes place, the cuvettes 18 of the cuvettarium 16 are very precisely directed When the certain time has elapsed, the straightening device at the end of the vessel sinks and brings the cuvettarium 16 back into the rest position in the cuvettarium frame 15, after which the cuvettarium frame is pushed forward either mechanically or manually in the measuring end. When the following cuvettarium 16 comes to the measuring point, the straightening part of the measuring end is directed and closes the following Cuvettari in order to be in the measuring position.

The reagent block 33 (Pig. 11) and the Cuvettarium 32 (Pig. 12 and 13) are of different designs, e.g. .9 sample tubes or Devices containing cuvettes from and into which 9 different Can pipette samples. In the reagent block 33 or in the cuvettarium 32, reagents can be stored as dry substances or as solutions.

Reage Qz / true. Solutions are obtained from the dry matter if suitable solutions are added to them in the reagent block 33 or in the cuvettarium 32. Thus, in the reagent block 33 or in the cuvettarium 32, finished dosages of the same or different reagents can be stored in a certain amount.

Through the lid of the reagent block 33 and the cuvettarium 32 each tube 33a of the reagent block or each cuvette 32a can be used

Times

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of the Cuvett & rium 32 at the same time verochlinsaea. Through this cover 31 one can be used. the evaporation of the liquids or the contamination of the substances in the roots 33 & and the cuvettes 32a beil udli before η prevent. Secondly, each cuvette 32a of the cuvette 32 can be closed through the cover Jl in such a way that the "Jk> denflache 31b of each ¹ 'peg 31a in the cover 31 presses on peg 31a on the corresponding cuvette 32a of the cuvettarium 32 on the father side on the otelle, through which the light from the liquid continues to the detector 4-0. The cover 31 of the cuvette 32 is a unitary disk, the holes of which are the downwardly directed pegs 31a, the through-holes of which are closed at the lower end by transparent bottom surfaces 31b. The pegs 31a of the cover 31 are shaped so that - when the cover is in place in the cuvette between the vertical or inclined outer wall of the pegs 31a and the cover 31 and the inner walls of the upper parts of the corresponding cuvettes 32a of the cuvette 32, a free space remains .

If one measures the absorbance of the liquid in the cuvettes 32a of the cuvette 32 in the photometer according to the invention, which functions according to the vertical measuring principle, is the length of the light path of the? Liquid located in the cuvette 32a is the distance h, which ^{denotes the '.' / eg between the bottom surface 1} 36 of the cuvette 32a and the floor plan 31b of the pin 31a in the lid 31. 'This distance h can be πιειη by producing different lids 31 to Regulate the cuvetturium if the length of the pegs 31a in the cover 31 of the cuvettarium changes, or by making cuvettaria 32 of different heights.In addition, the walls of the pegs 31a in the cover 31 can be made opaque, whereby the light only passes through the transparent bottom surfaces 31b of the cones 31a in the cover 31 äes · Cuvettariums 32 can run.

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Thanks to the construction according to the invention, there are height errors iu the liquid column !!, caused by pipetting, splashing the liquid as drips on the inner walls of the cuvettarium 32 etc. no errors in the length of the light path cause..

If the surface of the column of liquid in the Cuvette des Cuvett: iriuins "is curved or inclined, this can be the case in photometry, which works according to the vertical measuring principle

'32 cause errors in the measurement result. When the guvettarium / by the lid 31 is closed, the bottom surfaces 36 in each cuvette are aligned with the bottom surfaces 31b of the pegs 31a in the cover 31 of the cuvettarium 32. Here, the light runs in a vertical direction through the rectified plane surfaces and the liquid column located therebetween, from which it follows that the aforementioned imperfections in the measurement results fail.

Occasionally, in connection with shaking or pipetting, bubbles 37 form on the free surface of the liquid, such Bubbles cannot interfere with the photometric measurement, though the measurement is carried out according to the vertical measuring principle, in which the light passes through the bottom surface 36 of the cuvette 32a of the cuvettarium

runs and then through the following column of liquid and finally through the free area of the liquid and the air to the detector 40. When the cuvettarium 32 is closed by the cover 31 of the cuvettarium, the bottom surface 31b of the cuvettarium 32 presses each pin 31a Peg 31a in the cover 31 in the corresponding cuvette 32a on the underside el · ν some free liquid surface 34. Here, the bubbles 37 possibly located in the liquid surface move to the free liquid surface, the

509820/0744 ^mz

between the inner wall of the cuvette y <2 & the cuvetturium 32 and the outer wall of the pin 31a in the cover of the cuvettarium 32 "binds.

The errors described above can also be improved if necessary, that instead of the lid of the Cuvettarium 32 the seat of the protected detector 40 is passed into each cuvette 32a of the cuvettarium 32 somewhat below the pool of liquid 34.

Much the Beersian Ges & z the absorbance of the liquid in straight and linear respect is equal to the length of the light path in the cuvette. W-.im man im. Cuvettarium 32 measures in which. If the lid 31 is not used, in the photometry, which works according to the vertical measurement principle, the light paths of the cuvette 32a of the cuvette 32 are equal to the height of the liquid columns in the cuvettes 32a, which in turn depend straight and linearly on the liquid volume poured into the cuvette. If too few reagents are pipetted on the assumption that the sample amount is correct, the light path in the cuvette is shortened and the absorbance increases with the light wave of the cuvette per unit of measurement. If, on the other hand, the reagents are pipetted too much, the absorbance decreases with the light path of the pipette per unit of measure. In the photometry, which works according to the vertical measuring principle - when the cover 31 of the cuvette 32 is not needed in the case of Hessen, the previously described way turns the light path of the cuvettes 32a of the cuvetturium 32 into a constant that when piping the reagent or reagents possible errors no errors in the absorbance in the reaction mixture. Only if the sample is incorrectly pipetted will the absorbance of the reagent mixture be incorrect. .

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In multi-channel lhotometry, which works according to the vertical measuring principle, the detectors are in the immediate vicinity of the preamplifier 41 at the measuring end of the photometer or there. η 40 to the preamplifier 41 no error signals arise. The straightener 42 of the Cuvett <x.rX.um 32 at the measuring end of the photometer lifts the Cuvetti.rium 32 up to the plane surface 43 of the B-eating end. When the love movement ends, this flat surface presses the cover 31 of the cuvettarium tightly against the, Cuvetturiuui 32. It is necessary to ensure that the cover 31 of the cuvettarium 32 is securely around. Plata is, whereby z, B. the light path of each Cuvette 32a deü Cuvettariums 32 is the same length. The same or different wavelengths can be controlled in the light-controlling Piibwin 44 coming to the end of the tank. This method allows measurements in a cuvette 32a of the cuvettarium 32 at the same or different wavelengths

The invention is of course similar to those previously described Examples are limited but may be within the scope of the for the claims are changed.

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Claims (1)

- i J - Patent claims: ij Procedure for the automatic reading and registration of virological, bacteriological and hematological reaction results or for spectrophotometric measurement and recording · of chemical color reactions from changes in light permeability caused due to the changes in the specified intensity of light, characterized by that when measuring the cuvette in place, the guvette row or the cuvette group by the in each cuvette (C, 10) liquid column to be read or measured and through the delimiting cuvette base into the reading device (2,8) ■ light is passed through each cuvette (C, 10) in the direction of the longitudinal axis of the cuvette (C, 10) from one side of the Cuvette located light source runs to the detector (3,14-) located on the other side of the cuvette Light in the direction of the longitudinal axis of the cuvette the results of the serological, bacteriological and hamatolofeischen reactions or the color reactions on the cuvette or cuvettes in place can be read from the. located on the ground or Precipitation not sunk in the liquid column ^ Opacities etc. or the changes in the color nLar liquid in the cuvette or cuvettes, the change in light transmission due to the change in the precise intensity of the light caused. 2. The method according to claim 1, characterized in that g e k e η η ζ e.i c hn e t, -that with each cuvette (C, 10) a light source or over All cuvettes have an all-encompassing, uniform source of light and it is best to have a detector (3) for each cuvette. 3. The method according to claim 1, characterized in that that in the reading device adapted to the support (l) 509820/0744 d ORIGIN- sources (2) and a corresponding detector for each light source cia (i) are in rows, and that the support (l) from one to the other ixn the guvette row slidable iot, or that the cuvette ge stell "can be movable and the support stays in place, whereby the reading of the cuvettes (C) 'or measuring is carried out for each loan. 4. The method according to claim 1, characterized in that for measuring each cuvette (32a) of the Cuvett..rium (32) by cover (31) is closed so that the bottom surface (31b) Jceden pin (31a) in the cover (31) the pin (31a) in the corresponding cuvette (32a) of the cuvettarium © (32) located free liquid surface (34) on the underside or in the cuvettes (32a) dbB Cuvettariumar (32) the tip of the protected detector ( 40) is guided in each cuvette (32a) of the cuvette (i2) , free liquid area (34) on the underside at the point through which the light from the liquid runs to the detector (40). 5. Device for carrying out the method according to claim. 1, characterized in that the device in a cuvettarium rack (15) includes fitted cuvettaria (16), their fixed or detachable adapted cuvettes (18) in the position of one another like the detectors located in the measuring device and of these corresponding light sources are attached, and that the Hess window (21) of the cuvettes (18) suitably auf.der Outside surrounded by a cylinder-like protective part or edge is. - 6. Device according to claim 5 »marked thereby-α e t, that the cuvettes (18) of the tuvetturium (16) are at the same time can be closed by a cover (20), the pin of which the have the same position to one another as the position of the cuvettes (18) of the CuvettArium (16) to one another. 509820 / 07A4 7. Device according to the claims Ό or 6, characterized GEK e η η ζ calibration η et that the lower part (2J) of the cuvette (l8) de ο jJuvett: .. riuniu (16), ura directing the cuvettes (18 ) which has the shape of a blunt kegt. 8. "Device according to claims 5-7. Thereby g e Ic e nn ~ Seichnet that on the inner wall of the cuvette (18) one or smaller cassettes (24) are in the direction of the longitudinal axis of the cuvette, 9. Device according to claim 5, characterized thereby gekennzeiohn e t that the bottom of the used Cuvette (Ο, Ι.Ο) or in each the cuvette group is concave on the inside and on the outside is even. . . . ' 10 .. device according to claim 5 »characterized thereby, that the bottom of the cuvettes (18) of the cuvettariuras (16) is flat 11. Device according to Pa.tentansx) ruch 5, characterized in that in the cuvettarium frame (215) there is a projection (25) which is inserted into the cutout or opening (26) in the cover plate (17) of the cuvettarium (16) fits so that ¹ the cuvettarium (16) can only be placed in one position in the cuvettarium frame (15). 12. Device according to claim 5 »thereby marked chn e t that in the cover plate (17) of the Cuvettarium (16) Code (27) is what the cuvettarium is from in the measuring device and / or visually (16) is to be investigated.; · 13. Device according to claim 5 »marked thereby! η e t that the Cuvettε ^ rium frame (15) can be heated, ' Device according to claim 5, characterized by g ^ ekennzeich-. net that the device comprises a! Öeckel, which has a uniform BATH ORIGINAL 509820/0744 lent disc, in. the hollow abwar tegericshfce te pegs (31a) are formed, the bottoms of which are transparent, in the direction of the decicle disc and at the same distance from the bottom surfaces there (31b) and the outer diameter of the ^ a ^ fen 31a) whose bottom areas (jib) are smaller than the inside diameter corresponding to the lid dex ¹ guvettes (32a) dee Cuvettt riums (j2), 15. Lid according to claim 14, characterized in that that the pins (31a) of the cover (31) are cylindrical. 16. Lid according to claim 14, characterized in that that the pegs (31a) of the Beckeis (31) narrow downwards in the form of a circular cone. 17. Lid according to claim 14, characterized in that that the inner walls of the pin (31a) of the cover (31) are completely or partially impermeable to light: 5098 2 0/0744