DE3751859D1 - Durchflusszytometrie-Verfahren zur Klassifikation von Leukozyten und Reagenzien dafür - Google Patents

Durchflusszytometrie-Verfahren zur Klassifikation von Leukozyten und Reagenzien dafür

Info

Publication number
DE3751859D1
DE3751859D1 DE3751859T DE3751859T DE3751859D1 DE 3751859 D1 DE3751859 D1 DE 3751859D1 DE 3751859 T DE3751859 T DE 3751859T DE 3751859 T DE3751859 T DE 3751859T DE 3751859 D1 DE3751859 D1 DE 3751859D1
Authority
DE
Germany
Prior art keywords
leukocytes
classification
flow cytometry
cytometry method
reagents therefor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3751859T
Other languages
English (en)
Other versions
DE3751859T2 (de
Inventor
Syouhei Matsuda
Takashi Sakata
Tomoyuki Kuroda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sysmex Corp
Original Assignee
Sysmex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP61282698A external-priority patent/JPH0650310B2/ja
Priority claimed from JP61282699A external-priority patent/JPH0664053B2/ja
Application filed by Sysmex Corp filed Critical Sysmex Corp
Application granted granted Critical
Publication of DE3751859D1 publication Critical patent/DE3751859D1/de
Publication of DE3751859T2 publication Critical patent/DE3751859T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N2015/011—
    • G01N2015/016—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4704—Angular selective
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4704—Angular selective
    • G01N2021/4707—Forward scatter; Low angle scatter
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
DE3751859T 1986-11-27 1987-09-08 Durchflusszytometrie-Verfahren zur Klassifikation von Leukozyten und Reagenzien dafür Expired - Fee Related DE3751859T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP61282698A JPH0650310B2 (ja) 1986-11-27 1986-11-27 フローサイトメトリーによる白血球の分類方法
JP61282699A JPH0664053B2 (ja) 1986-11-27 1986-11-27 フロ−サイトメトリ−による白血球の分類方法

Publications (2)

Publication Number Publication Date
DE3751859D1 true DE3751859D1 (de) 1996-08-22
DE3751859T2 DE3751859T2 (de) 1997-01-09

Family

ID=26554721

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3751859T Expired - Fee Related DE3751859T2 (de) 1986-11-27 1987-09-08 Durchflusszytometrie-Verfahren zur Klassifikation von Leukozyten und Reagenzien dafür
DE87113119T Expired - Fee Related DE3786233T2 (de) 1986-11-27 1987-09-08 Durchfluss-Zytometrieverfahren zum Klassifizieren von Leukozyten und dazu angewandte Reagenzien.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE87113119T Expired - Fee Related DE3786233T2 (de) 1986-11-27 1987-09-08 Durchfluss-Zytometrieverfahren zum Klassifizieren von Leukozyten und dazu angewandte Reagenzien.

Country Status (4)

Country Link
US (1) US5039613A (de)
EP (2) EP0268766B1 (de)
CA (1) CA1309328C (de)
DE (2) DE3751859T2 (de)

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US5179026A (en) * 1986-11-27 1993-01-12 Toa Medical Electronics Co., Ltd. Method of classifying leukocytes by flow cytometry and reagents used in the method
JP2635126B2 (ja) * 1988-09-30 1997-07-30 東亜医用電子株式会社 核の分葉指数を求めるための粒子分析装置及び方法
FR2646914A1 (fr) * 1989-05-12 1990-11-16 Leca Procede et dispositif d'identification et de numerisation de cellules dans du lait
CA2016699C (en) * 1989-05-15 2003-11-18 Paul N. Marshall Lytic agents and uses thereof
JP2941041B2 (ja) * 1990-11-16 1999-08-25 シスメックス株式会社 フローサイトメトリーによる白血球の分類方法
JP2927979B2 (ja) * 1991-02-22 1999-07-28 シスメックス株式会社 フローサイトメトリーによる赤芽球の分類方法
JP3070968B2 (ja) * 1991-05-14 2000-07-31 シスメックス株式会社 尿中の細胞分析用試薬及び方法
JP3098273B2 (ja) * 1991-05-14 2000-10-16 シスメックス株式会社 尿中の細胞分析装置
JP3048260B2 (ja) * 1991-07-29 2000-06-05 シスメックス株式会社 白血球分類計数用試料調製方法
JP3067849B2 (ja) * 1991-07-29 2000-07-24 シスメックス株式会社 白血球分類計数用試料調製方法
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
US5360739A (en) * 1991-12-05 1994-11-01 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
EP0626067A4 (de) * 1992-02-07 1997-06-25 Abbott Lab Verfahren zur genauen zählung und empfindlichen qualifizierung heterogener zellpopulationen unter zytolytischen behandlungsbedingungen.
US5334450A (en) * 1992-05-20 1994-08-02 The Dow Chemical Company Weatherable styrenic film structures with intermediate tie layer and laminates thereof
DE69327775T2 (de) * 1992-11-19 2000-06-21 Sysmex Corp Verfahren zur Vorbehandlung für Blutanalyse
US5585246A (en) * 1993-02-17 1996-12-17 Biometric Imaging, Inc. Method for preparing a sample in a scan capillary for immunofluorescent interrogation
DE4309328C2 (de) * 1993-03-18 1998-03-12 Volker Ost Verfahren zur Differenzierung, Konzentrationsbestimmung und Sortierung von Erythrozyten, Thrombozyten und Leukozyten
JP3467310B2 (ja) * 1994-04-21 2003-11-17 シスメックス株式会社 白血球分析用試薬及び白血球の分類方法
JP3783808B2 (ja) * 1997-05-19 2006-06-07 シスメックス株式会社 白血球分類計数用試薬
US20030119080A1 (en) * 2001-10-15 2003-06-26 Mangano Joseph A. Strategies for the identification and isolation of cancer stem cells and non-cancerous stem cells
JP2005043365A (ja) * 2003-07-21 2005-02-17 Bayer Corp 白血球を減少させた血液バンク製剤における残留白血球を分析するための試薬組成物
WO2005085842A2 (en) * 2004-03-09 2005-09-15 Universite Libre De Bruxelles Method for the simultaneous detection of populations of several different biological entities using flow cytometry, device and computer program therefor
US7674622B2 (en) * 2006-12-22 2010-03-09 Abbott Laboratories, Inc. Method for determination of nucleated red blood cells and leukocytes in a whole blood sample in an automated hematology analyzer
CN101349644B (zh) * 2007-07-20 2012-06-27 深圳迈瑞生物医疗电子股份有限公司 一种白细胞分类试剂和其使用方法
CN101475754A (zh) * 2008-01-04 2009-07-08 深圳迈瑞生物医疗电子股份有限公司 不对称菁类荧光染料,组合物及在生物样品染色中的用途
CN101602762B (zh) * 2008-06-10 2013-10-16 深圳迈瑞生物医疗电子股份有限公司 不对称菁类化合物、其制备方法及应用
CN101726579B (zh) * 2008-10-17 2014-06-18 深圳迈瑞生物医疗电子股份有限公司 血液检测试剂和方法
CN101750274B (zh) * 2008-12-17 2014-06-25 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法
CN101988082B (zh) * 2009-07-31 2015-04-08 深圳迈瑞生物医疗电子股份有限公司 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法
CN106383082B (zh) * 2015-10-14 2018-10-19 北京信息科技大学 一种流式细胞仪无液路情况的光路调整装置及方法
CN113454453A (zh) * 2019-05-08 2021-09-28 深圳迈瑞生物医疗电子股份有限公司 全血样本分析仪、全血样本分析方法及其装置和存储介质

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Also Published As

Publication number Publication date
DE3786233T2 (de) 1993-11-25
EP0268766A2 (de) 1988-06-01
EP0268766A3 (en) 1988-12-14
EP0483116A3 (en) 1992-07-22
EP0268766B1 (de) 1993-06-16
CA1309328C (en) 1992-10-27
EP0483116B1 (de) 1996-07-17
US5039613A (en) 1991-08-13
EP0483116A2 (de) 1992-04-29
DE3751859T2 (de) 1997-01-09
DE3786233D1 (de) 1993-07-22

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Legal Events

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8339 Ceased/non-payment of the annual fee