DE68915675D1 - Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen. - Google Patents

Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.

Info

Publication number
DE68915675D1
DE68915675D1 DE68915675T DE68915675T DE68915675D1 DE 68915675 D1 DE68915675 D1 DE 68915675D1 DE 68915675 T DE68915675 T DE 68915675T DE 68915675 T DE68915675 T DE 68915675T DE 68915675 D1 DE68915675 D1 DE 68915675D1
Authority
DE
Germany
Prior art keywords
vitamin
proteins
purification
dependent proteins
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68915675T
Other languages
English (en)
Other versions
DE68915675T3 (de
DE68915675T2 (de
Inventor
Sau-Chi Betty Yan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26943089&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE68915675(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of DE68915675D1 publication Critical patent/DE68915675D1/de
Publication of DE68915675T2 publication Critical patent/DE68915675T2/de
Publication of DE68915675T3 publication Critical patent/DE68915675T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
DE68915675T 1988-10-04 1989-10-02 Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen. Expired - Lifetime DE68915675T3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25327988A 1988-10-04 1988-10-04
US07/393,281 US4981952A (en) 1988-10-04 1989-08-16 Method for the purification of vitamin K-dependent proteins

Publications (3)

Publication Number Publication Date
DE68915675D1 true DE68915675D1 (de) 1994-07-07
DE68915675T2 DE68915675T2 (de) 1994-10-20
DE68915675T3 DE68915675T3 (de) 2002-08-14

Family

ID=26943089

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68915675T Expired - Lifetime DE68915675T3 (de) 1988-10-04 1989-10-02 Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.

Country Status (13)

Country Link
US (1) US4981952A (de)
EP (1) EP0363126B2 (de)
JP (1) JP2848461B2 (de)
KR (1) KR0139209B1 (de)
AT (1) ATE106406T1 (de)
AU (1) AU635222B2 (de)
CA (1) CA1314011C (de)
DE (1) DE68915675T3 (de)
DK (1) DK176090B1 (de)
ES (1) ES2054019T5 (de)
HU (1) HU204538B (de)
IE (1) IE63765B1 (de)
IL (1) IL91822A (de)

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DE3833936C1 (de) * 1988-10-05 1989-09-21 Henning Berlin Gmbh Chemie- Und Pharmawerk, 1000 Berlin, De
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IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
MY110664A (en) * 1992-05-21 1999-01-30 Lilly Co Eli Protein c derivatives
DK38293D0 (da) * 1993-03-31 1993-03-31 Novo Nordisk As Fremstilling af proteiner
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
DE4435520A1 (de) * 1994-10-04 1996-04-11 Immuno Ag Verfahren zur Trennung von rekombinantem pro-Faktor IX von rekombinantem Faktor IX
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US5843731A (en) * 1996-09-05 1998-12-01 Asahi Kogaku Kogyo Kabushiki Kaisha Method for purifying plasmid DNA on calcium phosphate compound
US5910584A (en) * 1996-09-05 1999-06-08 Asahi Kogaku Kogyo Kabushiki Kaisha Method for isolating plasmid DNA
EP1557463A1 (de) * 1997-04-28 2005-07-27 Eli Lilly & Company Verbesserte Methoden zur Verarbeitung von aktiviertem Protein C
US6630137B1 (en) 1997-04-28 2003-10-07 Eli Lilly And Company Activated protein C formulations
PE84799A1 (es) * 1997-04-28 1999-09-16 Lilly Co Eli Metodos mejorados para el procesamiento de proteina c activada
ZA984698B (en) * 1997-06-05 2000-02-01 Lilly Co Eli Methods for treating thombotic disorders.
HUP0001237A3 (en) 1997-10-20 2002-01-28 Lilly Co Eli Methods for treating vascular disorders
WO2000023101A1 (en) 1998-10-22 2000-04-27 Eli Lilly And Company Methods for treating sepsis
ATE320263T1 (de) 1998-11-13 2006-04-15 Lilly Co Eli Verwendung von menschliche protein c zur herstellung eines arzneimittels zur behandlung von heparininduzierter thrombozytopenie
ES2195655T3 (es) 1998-11-20 2003-12-01 Lilly Co Eli Procedimiento para tratar fiebre hemorragica virica con proteina c.
ATE232739T1 (de) 1998-11-23 2003-03-15 Lilly Co Eli Protein c zur behandlung von sichelzellanämie und thalassämie
US7204981B2 (en) * 2000-03-28 2007-04-17 Eli Lilly And Company Methods of treating diseases with activated protein C
JP2004511428A (ja) 2000-05-24 2004-04-15 イーライ・リリー・アンド・カンパニー 凝固亢進状態を処置するための製剤および方法
AU2001272736A1 (en) * 2000-07-21 2002-02-05 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Method of purifying calcium ion-binding protein
US20040198652A1 (en) * 2001-04-24 2004-10-07 Carter J. Paul Methods and compositions for preventing and treating septic shock and endotoxemia
JP2004535461A (ja) * 2001-07-19 2004-11-25 ディーエムアイ バイオサイエンシズ インコーポレイテッド プロテインｃの不活性化を抑制するための銅キレート剤の使用
JP4628618B2 (ja) * 2001-09-26 2011-02-09 富士フイルム株式会社 撮像光学系
WO2003075834A2 (en) * 2002-03-08 2003-09-18 Eli Lilly And Company Activated protein c formulations
WO2004056309A2 (en) 2002-12-05 2004-07-08 Socratech L.L.C. Neuroprotective activity of activated protein c is independent of its anticoagulant activity
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
US9192657B2 (en) * 2003-07-08 2015-11-24 The Scripps Research Institute Activated protein C variants with normal cytoprotective activity but reduced anticoagulant activity
WO2005007820A2 (en) 2003-07-08 2005-01-27 The Scripps Research Institute Activated protein c variants with normal cytoprotective activity but reduced anticoagulant activity
CA2574598A1 (en) * 2004-07-23 2006-02-09 The University Of Rochester Activated protein c inhibits undesirable effects of plasminogen activator in the brain
JP2008525381A (ja) 2004-12-23 2008-07-17 ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少
EP1841863B1 (de) * 2005-01-14 2010-08-04 Bayer HealthCare LLC Verfahren zur reinigung von factor vii
DK1898945T3 (da) * 2005-06-24 2013-03-25 Drugrecure Aps Luftvejsadministration af site-inaktiveret FVIIA ved inflammatoriske tilstande, der påvirker luftvejene
WO2007071767A1 (en) * 2005-12-23 2007-06-28 Novo Nordisk Health Care Ag Purification of vitamin k-dependent polypeptides using preparative reverse phase chromatography (rpc)
TWI428448B (zh) 2006-03-24 2014-03-01 Syntonix Pharmaceuticals Inc 作為第九因子（ｆａｃｔｏｒ ｉｘ）原肽處理酶之ｐｃ５
US20100284997A1 (en) * 2006-10-31 2010-11-11 Griffin John H Dosing regimen of activated protein c and variants having reduced anticoagulant activity
EP2125866B1 (de) * 2007-02-28 2013-05-22 Baxter International Inc. Verfahren zur reinigung von mit sulfatierten und/oder phosphorylierten molekülen angereichtem rekombinantem blutgerinnungsfaktor ix
CA3090908A1 (en) 2007-04-26 2008-11-06 Aptevo Biotherapeutics Llc Recombinant vitamin k dependent proteins with high sialic acid content and methods of preparing same
DK3597659T3 (da) 2007-07-09 2023-04-03 Genentech Inc Forhindring af disulfidbindingsreduktion under rekombinant produktion af polypeptider
KR101821143B1 (ko) * 2008-12-02 2018-01-23 노보 노르디스크 헬스 케어 악티엔게젤샤프트 폴리펩티드 정제
JP5833448B2 (ja) 2008-12-19 2015-12-16 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル セリンプロテアーゼ誘導体および血液凝固疾患の予防または処置における使用
EP2199387A1 (de) 2008-12-19 2010-06-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Serinproteasederivate und deren Verwendung zur Vorbeugung und/oder Behandlung von Blutgerinnungsstörungen
EP2494040B1 (de) 2009-10-30 2018-08-29 Aptevo BioTherapeutics LLC Verfahren zur herstellung rekombinanter vitamin-k-abhängiger proteine
US9896677B2 (en) 2010-01-18 2018-02-20 Novo Nordisk Health Care Ag Purification of blood coagulation factors
PT2563805T (pt) * 2010-04-29 2020-05-18 Baxalta Inc Método de purificação de proteínas que se ligam a catiões divalentes em resina de permuta aniónica
EP2691411B1 (de) 2011-03-29 2020-02-26 GlaxoSmithKline LLC Puffersystem zur proteinreinigung
EP2753706A4 (de) * 2011-09-06 2015-08-12 Medimmune Llc Verfahren zur verarbeitung von gerinnungsfaktoren
WO2013053887A1 (en) * 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
AU2012322949B2 (en) * 2011-10-14 2015-07-02 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
US20150150954A1 (en) 2012-07-04 2015-06-04 The University Of Sydney Treatment of inflammatory skin disorders
JP6571011B2 (ja) * 2013-03-15 2019-09-04 バクスアルタ インコーポレイテッド 陰イオン交換クロマトグラフィーによるビタミンｋ依存性タンパク質の精製方法
CN106573040B (zh) 2014-04-16 2021-07-06 Zz生物技术有限责任公司 治疗异常皮肤瘢痕形成
WO2017118910A1 (en) * 2016-01-07 2017-07-13 Eio Biomedical Ltd Methods, compositions and kits for reducing tissue adhesions

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US4512992A (en) * 1980-06-13 1985-04-23 Burroughs Wellcome Co. Treatment with dialkoxy pyridopyrimidine compounds
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
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US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
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US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
DE3615558A1 (de) * 1986-05-09 1987-11-12 Behringwerke Ag Verfahren zur herstellung eines faktor v-konzentrats

Also Published As

Publication number Publication date
DE68915675T3 (de) 2002-08-14
AU635222B2 (en) 1993-03-18
CA1314011C (en) 1993-03-02
IE893159L (en) 1990-04-04
US4981952A (en) 1991-01-01
DK485589A (da) 1990-04-05
AU4251989A (en) 1990-04-12
IL91822A0 (en) 1990-06-10
HUT53373A (en) 1990-10-28
EP0363126B2 (de) 2002-03-06
HU204538B (en) 1992-01-28
EP0363126A2 (de) 1990-04-11
DK176090B1 (da) 2006-05-22
DE68915675T2 (de) 1994-10-20
IL91822A (en) 2001-06-14
ES2054019T5 (es) 2002-10-16
ATE106406T1 (de) 1994-06-15
EP0363126B1 (de) 1994-06-01
KR900006511A (ko) 1990-05-08
IE63765B1 (en) 1995-06-14
DK485589D0 (da) 1989-10-03
JP2848461B2 (ja) 1999-01-20
ES2054019T3 (es) 1994-08-01
EP0363126A3 (de) 1991-09-11
KR0139209B1 (ko) 1998-04-30
JPH02200180A (ja) 1990-08-08

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings