DE68915675T3 - Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen. - Google Patents

Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.

Info

Publication number
DE68915675T3
DE68915675T3 DE68915675T DE68915675T DE68915675T3 DE 68915675 T3 DE68915675 T3 DE 68915675T3 DE 68915675 T DE68915675 T DE 68915675T DE 68915675 T DE68915675 T DE 68915675T DE 68915675 T3 DE68915675 T3 DE 68915675T3
Authority
DE
Germany
Prior art keywords
vitamin
proteins
purification
dependent proteins
culture medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68915675T
Other languages
English (en)
Other versions
DE68915675T2 (de
DE68915675D1 (de
Inventor
Sau-Chi Betty Yan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eli Lilly and Co
Original Assignee
Eli Lilly and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26943089&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE68915675(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Eli Lilly and Co filed Critical Eli Lilly and Co
Application granted granted Critical
Publication of DE68915675D1 publication Critical patent/DE68915675D1/de
Publication of DE68915675T2 publication Critical patent/DE68915675T2/de
Publication of DE68915675T3 publication Critical patent/DE68915675T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
DE68915675T 1988-10-04 1989-10-02 Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen. Expired - Lifetime DE68915675T3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25327988A 1988-10-04 1988-10-04
US07/393,281 US4981952A (en) 1988-10-04 1989-08-16 Method for the purification of vitamin K-dependent proteins

Publications (3)

Publication Number Publication Date
DE68915675D1 DE68915675D1 (de) 1994-07-07
DE68915675T2 DE68915675T2 (de) 1994-10-20
DE68915675T3 true DE68915675T3 (de) 2002-08-14

Family

ID=26943089

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68915675T Expired - Lifetime DE68915675T3 (de) 1988-10-04 1989-10-02 Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.

Country Status (13)

Country Link
US (1) US4981952A (de)
EP (1) EP0363126B2 (de)
JP (1) JP2848461B2 (de)
KR (1) KR0139209B1 (de)
AT (1) ATE106406T1 (de)
AU (1) AU635222B2 (de)
CA (1) CA1314011C (de)
DE (1) DE68915675T3 (de)
DK (1) DK176090B1 (de)
ES (1) ES2054019T5 (de)
HU (1) HU204538B (de)
IE (1) IE63765B1 (de)
IL (1) IL91822A (de)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
DE3833936C1 (de) * 1988-10-05 1989-09-21 Henning Berlin Gmbh Chemie- Und Pharmawerk, 1000 Berlin, De
DE3911629A1 (de) * 1989-04-10 1990-10-11 Behringwerke Ag Verfahren zur abtrennung von toxinen aus proteinloesungen
IL97312A (en) 1990-02-23 1999-01-26 Lilly Co Eli A method of generating a polypeptide in an eukaryotic vector AND recombinant surrogate cell containing an enhanced transcriptional control unit based on the primary late adenovirus coefficient
JP3418621B2 (ja) * 1991-03-01 2003-06-23 アヴェンティス ベーリング エルエルシー Ｉｘ因子の調製
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
MY110664A (en) * 1992-05-21 1999-01-30 Lilly Co Eli Protein c derivatives
DK38293D0 (da) * 1993-03-31 1993-03-31 Novo Nordisk As Fremstilling af proteiner
US5618714A (en) * 1993-12-15 1997-04-08 Eli Lilly And Company Methods for producing protein C
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
DE4435520A1 (de) * 1994-10-04 1996-04-11 Immuno Ag Verfahren zur Trennung von rekombinantem pro-Faktor IX von rekombinantem Faktor IX
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US5843731A (en) * 1996-09-05 1998-12-01 Asahi Kogaku Kogyo Kabushiki Kaisha Method for purifying plasmid DNA on calcium phosphate compound
US5910584A (en) * 1996-09-05 1999-06-08 Asahi Kogaku Kogyo Kabushiki Kaisha Method for isolating plasmid DNA
KR100564189B1 (ko) * 1997-04-28 2006-03-27 일라이 릴리 앤드 캄파니 활성화 프로틴ｃ의 가공을 위한 개선된 방법
US6630137B1 (en) * 1997-04-28 2003-10-07 Eli Lilly And Company Activated protein C formulations
EP1557463A1 (de) * 1997-04-28 2005-07-27 Eli Lilly & Company Verbesserte Methoden zur Verarbeitung von aktiviertem Protein C
EA199901112A1 (ru) * 1997-06-05 2000-06-26 Эли Лилли Энд Компани Способы лечения тромботических нарушений
HUP0001237A3 (en) * 1997-10-20 2002-01-28 Lilly Co Eli Methods for treating vascular disorders
JP2002527490A (ja) 1998-10-22 2002-08-27 イーライ・リリー・アンド・カンパニー 敗血症を治療する方法
ES2259248T3 (es) 1998-11-13 2006-09-16 Eli Lilly And Company Uso de la proteina c humana para la preparacion de un medicamento para el tratamiento de trombocitopenia inducida por heparina.
IL142220A0 (en) 1998-11-20 2002-03-10 Lilly Co Eli Method of treating viral hemorrhagic fever
DE69905489T2 (de) 1998-11-23 2003-09-11 Lilly Co Eli Protein c zur behandlung von sichelzellanämie und thalassämie
US7204981B2 (en) * 2000-03-28 2007-04-17 Eli Lilly And Company Methods of treating diseases with activated protein C
JP2004511428A (ja) 2000-05-24 2004-04-15 イーライ・リリー・アンド・カンパニー 凝固亢進状態を処置するための製剤および方法
US6869934B2 (en) * 2000-07-21 2005-03-22 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Method of purifying calcium ion-binding protein
WO2002085117A1 (en) * 2001-04-24 2002-10-31 Eisai Co., Ltd. Methods and compositions for preventing and treating septic shock and endotoxemia
WO2003007686A2 (en) * 2001-07-19 2003-01-30 Dmi Biosciences, Inc. Use of copper chelators to inhibit the inactivation of protein c
JP4628618B2 (ja) * 2001-09-26 2011-02-09 富士フイルム株式会社 撮像光学系
AU2003213146A1 (en) * 2002-03-08 2003-09-22 Eli Lilly And Company Activated protein c formulations
WO2004056309A2 (en) 2002-12-05 2004-07-08 Socratech L.L.C. Neuroprotective activity of activated protein c is independent of its anticoagulant activity
US20070142272A1 (en) * 2003-01-24 2007-06-21 Zlokovic Berislav V Neuroprotective activity of activated protein c independent of its anticoagulant activity
US9192657B2 (en) * 2003-07-08 2015-11-24 The Scripps Research Institute Activated protein C variants with normal cytoprotective activity but reduced anticoagulant activity
CA2531695A1 (en) 2003-07-08 2005-01-27 The Scripps Research Institute Activated protein c variants with normal cytoprotective activity but reduced anticoagulant activity
US20080305100A1 (en) * 2004-07-23 2008-12-11 Zlokovic Berislav V Activated Protein C Inhibits Undesirable Effects of Plasminogen Activator in the Brain
CA2592054A1 (en) 2004-12-23 2006-06-29 Novo Nordisk Health Care Ag Reduction of the content of protein contaminants in compositions comprising a vitamin k-dependent protein of interest
US20090047723A1 (en) * 2005-01-14 2009-02-19 Bayer Healthcare Llc Method for purification of factor vii
WO2006136963A2 (en) * 2005-06-24 2006-12-28 Drugrecure Aps Airway administration of site-inactivated fviia in inflammatory conditions affecting the respiratory tract
WO2007071767A1 (en) * 2005-12-23 2007-06-28 Novo Nordisk Health Care Ag Purification of vitamin k-dependent polypeptides using preparative reverse phase chromatography (rpc)
WO2007112005A2 (en) 2006-03-24 2007-10-04 Syntonix Pharmaceuticals, Inc. Pc5 as a factor ix propeptide processing enzyme
CA2668187A1 (en) * 2006-10-31 2008-06-19 The Scripps Research Institute Dosing regimen of activated protein c and variants having reduced anticoagulant activity
EP2125866B1 (de) 2007-02-28 2013-05-22 Baxter International Inc. Verfahren zur reinigung von mit sulfatierten und/oder phosphorylierten molekülen angereichtem rekombinantem blutgerinnungsfaktor ix
WO2008134665A1 (en) 2007-04-26 2008-11-06 Inspiration Biopharmaceuticals, Inc. Recombinant vitamin k dependent proteins with high sialic acid content and methods of preparing same
ES2751022T3 (es) 2007-07-09 2020-03-30 Genentech Inc Prevención de la reducción de enlaces disulfuro durante la producción recombinante de polipéptidos
JP5788803B2 (ja) * 2008-12-02 2015-10-07 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー ポリペプチド精製
CN102325880B (zh) 2008-12-19 2014-10-01 国家健康与医学研究院 丝氨酸蛋白酶衍生物及其在凝血功能障碍的预防或治疗中的用途
EP2199387A1 (de) 2008-12-19 2010-06-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Serinproteasederivate und deren Verwendung zur Vorbeugung und/oder Behandlung von Blutgerinnungsstörungen
JP5851410B2 (ja) 2009-10-30 2016-02-03 シーエヌジェイ ホールディングス、インク． 組換えビタミンｋ依存性タンパク質の生成法
RU2012133474A (ru) 2010-01-18 2014-02-27 Ново Нордиск Хелс Кеа Аг Очистка факторов свертывания крови
KR101780518B1 (ko) * 2010-04-29 2017-09-21 박스알타 인코퍼레이티드 음이온 교환 수지상에서의 2가 양이온 결합 단백질의 정제 방법
JP6147726B2 (ja) 2011-03-29 2017-06-14 グラクソスミスクライン・リミテッド・ライアビリティ・カンパニーＧｌａｘｏＳｍｉｔｈＫｌｉｎｅ ＬＬＣ タンパク質精製のためのバッファー系
WO2013036445A1 (en) * 2011-09-06 2013-03-14 Medimmune Llc Methods for processing coagulation factors
EP2748180B1 (de) * 2011-10-14 2018-08-15 Baxalta GmbH Proteinaufreinigung mithilfe der anionenaustauschchromatographie
AU2012322948B2 (en) * 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
KR102068010B1 (ko) 2012-07-04 2020-01-20 제트제트 바이오테크 엘엘씨 염증성 피부 질환의 치료
AU2014230101B2 (en) * 2013-03-15 2018-04-19 Takeda Pharmaceutical Company Limited Purification method for vitamin K dependent proteins by anion exchange chromatography
ES2890675T3 (es) 2014-04-16 2022-01-21 Zz Biotech Llc APC para uso en el tratamiento de cicatrices cutáneas anormales
EP3400029B1 (de) * 2016-01-07 2022-03-02 Eio Biomedical Ltd Zusammensetzungen zur reduzierung von gewebeadhäsion

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4512992A (en) * 1980-06-13 1985-04-23 Burroughs Wellcome Co. Treatment with dialkoxy pyridopyrimidine compounds
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4518526A (en) * 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) * 1982-12-22 1986-07-08 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
DE3615558A1 (de) * 1986-05-09 1987-11-12 Behringwerke Ag Verfahren zur herstellung eines faktor v-konzentrats

Also Published As

Publication number Publication date
DK485589A (da) 1990-04-05
KR0139209B1 (ko) 1998-04-30
HU204538B (en) 1992-01-28
DE68915675T2 (de) 1994-10-20
AU4251989A (en) 1990-04-12
EP0363126B2 (de) 2002-03-06
JP2848461B2 (ja) 1999-01-20
DE68915675D1 (de) 1994-07-07
KR900006511A (ko) 1990-05-08
EP0363126B1 (de) 1994-06-01
IL91822A (en) 2001-06-14
AU635222B2 (en) 1993-03-18
ES2054019T5 (es) 2002-10-16
JPH02200180A (ja) 1990-08-08
IE893159L (en) 1990-04-04
ATE106406T1 (de) 1994-06-15
DK485589D0 (da) 1989-10-03
CA1314011C (en) 1993-03-02
US4981952A (en) 1991-01-01
DK176090B1 (da) 2006-05-22
IL91822A0 (en) 1990-06-10
HUT53373A (en) 1990-10-28
EP0363126A2 (de) 1990-04-11
EP0363126A3 (de) 1991-09-11
IE63765B1 (en) 1995-06-14
ES2054019T3 (es) 1994-08-01

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Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings