DE69311337D1 - Verfahren zur Reinigung von Proteinen - Google Patents

Verfahren zur Reinigung von Proteinen

Info

Publication number
DE69311337D1
DE69311337D1 DE69311337T DE69311337T DE69311337D1 DE 69311337 D1 DE69311337 D1 DE 69311337D1 DE 69311337 T DE69311337 T DE 69311337T DE 69311337 T DE69311337 T DE 69311337T DE 69311337 D1 DE69311337 D1 DE 69311337D1
Authority
DE
Germany
Prior art keywords
protein
sorbant
recovering
proteins
purification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69311337T
Other languages
English (en)
Inventor
Lance T Lusk
Henry Goldstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miller Brewing Co
Original Assignee
Miller Brewing Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miller Brewing Co filed Critical Miller Brewing Co
Application granted granted Critical
Publication of DE69311337D1 publication Critical patent/DE69311337D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
DE69311337T 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen Expired - Lifetime DE69311337D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/882,793 US5278284A (en) 1992-05-14 1992-05-14 Protein purification method
EP93115953A EP0646594B1 (de) 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen
JP5251960A JPH07145192A (ja) 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法

Publications (1)

Publication Number Publication Date
DE69311337D1 true DE69311337D1 (de) 1997-07-10

Family

ID=27235218

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69311337T Expired - Lifetime DE69311337D1 (de) 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen

Country Status (7)

Country Link
US (1) US5278284A (de)
EP (1) EP0646594B1 (de)
JP (1) JPH07145192A (de)
AT (1) ATE154033T1 (de)
DE (1) DE69311337D1 (de)
DK (1) DK0646594T3 (de)
ES (1) ES2105033T3 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
WO1996014571A1 (en) * 1994-11-08 1996-05-17 The Regents Of The University Of California Temperature-gradient osmometry
CA2167872C (en) * 1995-01-27 2007-04-03 Masaru Nakatani Adsorbent for removing interleukins and tumor necrosis factor, and process for removing the same
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US6764690B2 (en) * 1996-05-29 2004-07-20 Delsitech Oy Dissolvable oxides for biological applications
WO1999039739A1 (en) * 1998-02-05 1999-08-12 Henry M. Jackson Foundation For The Advancement Of Military Medicine Simplified method for removing free protein during preparation of protein-polysaccharide conjugates and vaccines using restricted-access media
US6132625A (en) * 1998-05-28 2000-10-17 E. I. Du Pont De Nemours And Company Method for treatment of aqueous streams comprising biosolids
US6203711B1 (en) 1999-05-21 2001-03-20 E. I. Du Pont De Nemours And Company Method for treatment of substantially aqueous fluids derived from processing inorganic materials
US20020173024A1 (en) * 2000-06-08 2002-11-21 Horwath Kathleen L. Nucleic acid sequences encoding type III tenebrio antifreeze proteins and method for assaying activity
US6780330B2 (en) 2001-03-09 2004-08-24 E. I. Du Pont De Nemours And Company Removal of biomaterials from aqueous streams
US20030026845A1 (en) * 2001-06-18 2003-02-06 Etzel Lisa R. Process for preparing protein isolate from milk, whey, colostrum, and the like
ES2322945T5 (es) * 2001-12-21 2012-10-17 Immunex Corporation Métodos para purficación de proteína
MXPA05005921A (es) * 2002-12-02 2005-10-19 Abgenix Inc Anticuerpos dirigidos a factor de necrosis tumoral y sus usos.
US8409647B2 (en) * 2008-08-12 2013-04-02 E. I. Du Pont De Nemours And Company Silica microgels for reducing chill haze
WO2012104339A1 (en) 2011-02-01 2012-08-09 Novo Nordisk A/S Purification of insulin
US10093674B2 (en) 2011-05-31 2018-10-09 Brandeis University Supramolecular nanofibers and hydrogels based on nucleic acids functionalized with nucleobases
WO2012166705A2 (en) * 2011-05-31 2012-12-06 Brandeis University Supramolecular nanofibers and hydrogels based on amino acid-nucleobase-glycoside conjugates
GB201411943D0 (en) 2014-07-03 2014-08-20 Univ Heriot Watt Process and protein product

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2769747A (en) * 1953-02-18 1956-11-06 Armour & Co Process of separation of siliceous impurities from insulin solutions
DE1442334B2 (de) * 1963-08-02 1980-04-24 Wilz-Pauls Geb. Pauls, Rosemarie, Basel (Schweiz) Verfahren zur Behandlung von Bier
GB1215928A (en) * 1967-04-28 1970-12-16 Unilever Ltd A process for reducing haze in beer
US3945988A (en) * 1974-07-16 1976-03-23 Pushpa Khann Process for isolation of insulin from plant source
GB1592702A (en) * 1976-10-28 1981-07-08 Asahi Chemical Ind Method of adsorbing a protein on a protein adsorbent comprising a porous copolymer of a cyano-group-containing monomer
JPS5810056A (ja) * 1981-07-10 1983-01-20 株式会社クラレ 血液浄化装置
DE3147842A1 (de) * 1981-12-03 1983-06-23 Hoechst Ag, 6230 Frankfurt "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
CA1178222A (en) * 1982-02-04 1984-11-20 Chang W. Chi Composition and method for stabilizing beverages against haze formation
HU190129B (en) * 1983-07-11 1986-08-28 Reanal Finomvegyszergyar,Hu Process for the isolation of carboxypeptidase b enzyme from mammal pancreas
US4528198A (en) * 1983-09-27 1985-07-09 Miller Brewing Company Preparation of low calorie beer with malt extract free of yeast lethal factors
US4636394A (en) * 1984-04-16 1987-01-13 J. E. Siebel Sons' Company, Inc. Method and compositions for chillproofing beverages
US4595578A (en) * 1985-03-22 1986-06-17 Scm Corporation Stabilized silica gel and process for making same
US4684530A (en) * 1985-10-04 1987-08-04 W. R. Grace & Co. Adsorption of proteins from fluids
JPS62207712A (ja) * 1986-03-05 1987-09-12 Fuji Debuison Kagaku Kk ビ−ルの安定化処理用含水シリカゲル
DE3633797C2 (de) * 1986-10-03 1995-08-10 Forssmann Wolf Georg Verfahren zur Gewinnung von biologisch aktiven Eiweissen (Peptiden) aus menschlichem und tierischem Blut (bzw. anderen Körperflüssigkeiten)
GB8707526D0 (en) * 1987-03-30 1987-05-07 Unilever Plc Amorphous silicas
JP2732252B2 (ja) * 1987-12-07 1998-03-25 株式会社ジェイ・エム・エス ヘモグロビン選択吸着剤
US4929560A (en) * 1988-02-03 1990-05-29 Damon Biotech, Inc. Recovery of tissue plasminogen activator
US5086167A (en) * 1988-11-28 1992-02-04 University Of Miami Method of separating proteins

Also Published As

Publication number Publication date
ES2105033T3 (es) 1997-10-16
US5278284A (en) 1994-01-11
DK0646594T3 (da) 1997-12-22
EP0646594B1 (de) 1997-06-04
EP0646594A1 (de) 1995-04-05
ATE154033T1 (de) 1997-06-15
JPH07145192A (ja) 1995-06-06

Similar Documents

Publication Publication Date Title
DE69311337D1 (de) Verfahren zur Reinigung von Proteinen
DE59409712D1 (de) Verfahren zur chromatographischen reinigung und trennung von nucleinsäuregemischen
DE68922427T2 (de) Verfahren zur Zurückgewinnung von sauerstoffreichem Gas.
DE59010793D1 (de) Verfahren zur inaktivierung von viren in blut und blutprodukten
DE69427602D1 (de) Verfahren zur Reinigung von Abgasen
DE69625026D1 (de) Verfahren zur Entfernung von Kohlensäure aus Gasen
EP0391526A3 (de) Reinigungsverfahren für Antikörper
DE69106100T2 (de) Verfahren zur Trennung durch Adsorption und Adsorber.
ATE161013T1 (de) Verfahren zum reinigen von 20 cs)-camptothecin
DE69017050D1 (de) Verfahren zur isolierung von faktor viii.
DE68914803T2 (de) Verfahren zur Reinigung von Abgasen.
ATE289289T1 (de) Verfahren zur reinigung von alkanolaminen
FI915813A0 (fi) Menetelmä luonnollisesti tuotetun kymosiinin talteenottamiseksi
BR8802849A (pt) Processo de separacao de misturas liquidas por pervaporacao
DE3780364D1 (de) Verfahren zur abscheidung von kohlendioxyd.
ATE163910T1 (de) Verfahren zur reinigung von 1,1-difluorethan
ATE173272T1 (de) Verfahren zur reaktivierung von gereinigten membranproteinen
ATE329902T1 (de) Reinigungsverfahren von imidoalkanpercarbonsäuren
ATE201387T1 (de) Verfahren zur reinigung von weissen phosphor
CA2022165A1 (en) Albumin purification
DE4495604T1 (de) Reaktive Membran zur Filtrierung und Reinigung von Gasen zwecks Abtrennung von Verunreinigungen und Verfahren unter Anwendung dieser Membran
DE3863411D1 (de) Verfahren zur reinigung von wasserstoff.
DE3781722D1 (de) Verfahren zur adsorptiven trennung von dichlortoluen.
ATE81496T1 (de) Verfahren zur reinigung von dihydroxybiphenylen.
DE69011762D1 (de) Verfahren zur Abtrennung und Reinigung von beta-Monoisopropylnaphthalen.

Legal Events

Date Code Title Description
8332 No legal effect for de