DE69519940T2 - Selbst dämpfende fluorescenz probe und verfahren - Google Patents

Selbst dämpfende fluorescenz probe und verfahren

Info

Publication number
DE69519940T2
DE69519940T2 DE69519940T DE69519940T DE69519940T2 DE 69519940 T2 DE69519940 T2 DE 69519940T2 DE 69519940 T DE69519940 T DE 69519940T DE 69519940 T DE69519940 T DE 69519940T DE 69519940 T2 DE69519940 T2 DE 69519940T2
Authority
DE
Germany
Prior art keywords
fluorescence
reporter molecule
target polynucleotide
molecule
oligonucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69519940T
Other languages
English (en)
Other versions
DE69519940D1 (de
Inventor
J Livak
J Flood
Jeffrey Maramaro
Bashar Mullah
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23333909&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69519940(T2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of DE69519940D1 publication Critical patent/DE69519940D1/de
Application granted granted Critical
Publication of DE69519940T2 publication Critical patent/DE69519940T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
DE69519940T 1994-11-16 1995-11-15 Selbst dämpfende fluorescenz probe und verfahren Expired - Lifetime DE69519940T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/340,558 US5538848A (en) 1994-11-16 1994-11-16 Method for detecting nucleic acid amplification using self-quenching fluorescence probe
PCT/US1995/014882 WO1996015270A1 (en) 1994-11-16 1995-11-15 Self-quenching fluorescence probe and method

Publications (2)

Publication Number Publication Date
DE69519940D1 DE69519940D1 (de) 2001-02-22
DE69519940T2 true DE69519940T2 (de) 2001-05-23

Family

ID=23333909

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69519940T Expired - Lifetime DE69519940T2 (de) 1994-11-16 1995-11-15 Selbst dämpfende fluorescenz probe und verfahren

Country Status (7)

Country Link
US (5) US5538848A (de)
EP (2) EP0792374B1 (de)
JP (3) JP4131749B2 (de)
AT (1) ATE198775T1 (de)
CA (1) CA2201756C (de)
DE (1) DE69519940T2 (de)
WO (1) WO1996015270A1 (de)

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EP0972848A2 (de) 2000-01-19
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EP0792374B1 (de) 2001-01-17
US5723591A (en) 1998-03-03
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US6258569B1 (en) 2001-07-10
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ATE198775T1 (de) 2001-02-15
JP4131749B2 (ja) 2008-08-13
US5538848A (en) 1996-07-23
US5876930A (en) 1999-03-02
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