US3598533A - Diagnostic paper strip - Google Patents
Diagnostic paper strip Download PDFInfo
- Publication number
- US3598533A US3598533A US818855A US3598533DA US3598533A US 3598533 A US3598533 A US 3598533A US 818855 A US818855 A US 818855A US 3598533D A US3598533D A US 3598533DA US 3598533 A US3598533 A US 3598533A
- Authority
- US
- United States
- Prior art keywords
- strip
- paper strip
- cholesterol
- rosin
- diagnostic paper
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004369 blood Anatomy 0.000 abstract description 25
- 239000008280 blood Substances 0.000 abstract description 25
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 24
- 235000012000 cholesterol Nutrition 0.000 abstract description 12
- 238000007598 dipping method Methods 0.000 abstract description 9
- 239000003960 organic solvent Substances 0.000 abstract description 7
- 238000001035 drying Methods 0.000 abstract description 5
- RSWGJHLUYNHPMX-UHFFFAOYSA-N Abietic-Saeure Natural products C12CCC(C(C)C)=CC2=CCC2C1(C)CCCC2(C)C(O)=O RSWGJHLUYNHPMX-UHFFFAOYSA-N 0.000 description 21
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 20
- KHPCPRHQVVSZAH-HUOMCSJISA-N Rosin Natural products O(C/C=C/c1ccccc1)[C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 KHPCPRHQVVSZAH-HUOMCSJISA-N 0.000 description 18
- KHPCPRHQVVSZAH-UHFFFAOYSA-N trans-cinnamyl beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OCC=CC1=CC=CC=C1 KHPCPRHQVVSZAH-UHFFFAOYSA-N 0.000 description 18
- 238000000034 method Methods 0.000 description 16
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 12
- MTHSVFCYNBDYFN-UHFFFAOYSA-N anhydrous diethylene glycol Natural products OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 12
- 239000004202 carbamide Substances 0.000 description 12
- 150000002148 esters Chemical class 0.000 description 12
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- -1 gum rosin Chemical class 0.000 description 9
- 210000002966 serum Anatomy 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 235000010985 glycerol esters of wood rosin Nutrition 0.000 description 4
- XUGISPSHIFXEHZ-UHFFFAOYSA-N 3beta-acetoxy-cholest-5-ene Natural products C1C=C2CC(OC(C)=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 XUGISPSHIFXEHZ-UHFFFAOYSA-N 0.000 description 3
- BTXXTMOWISPQSJ-UHFFFAOYSA-N 4,4,4-trifluorobutan-2-one Chemical compound CC(=O)CC(F)(F)F BTXXTMOWISPQSJ-UHFFFAOYSA-N 0.000 description 3
- BQACOLQNOUYJCE-FYZZASKESA-N Abietic acid Natural products CC(C)C1=CC2=CC[C@]3(C)[C@](C)(CCC[C@@]3(C)C(=O)O)[C@H]2CC1 BQACOLQNOUYJCE-FYZZASKESA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OVXRPXGVKBHGQO-UHFFFAOYSA-N abietic acid methyl ester Natural products C1CC(C(C)C)=CC2=CCC3C(C(=O)OC)(C)CCCC3(C)C21 OVXRPXGVKBHGQO-UHFFFAOYSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- XUGISPSHIFXEHZ-VEVYEIKRSA-N cholesteryl acetate Chemical compound C1C=C2C[C@@H](OC(C)=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 XUGISPSHIFXEHZ-VEVYEIKRSA-N 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- OVXRPXGVKBHGQO-UYWIDEMCSA-N methyl (1r,4ar,4br,10ar)-1,4a-dimethyl-7-propan-2-yl-2,3,4,4b,5,6,10,10a-octahydrophenanthrene-1-carboxylate Chemical compound C1CC(C(C)C)=CC2=CC[C@H]3[C@@](C(=O)OC)(C)CCC[C@]3(C)[C@H]21 OVXRPXGVKBHGQO-UYWIDEMCSA-N 0.000 description 3
- 239000005148 Cholesterol Benzoate Substances 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- BBJQPKLGPMQWBU-UHFFFAOYSA-N Palmitinsaeurecholesterylester Natural products C12CCC3(C)C(C(C)CCCC(C)C)CCC3C2CC=C2C1(C)CCC(OC(=O)CCCCCCCCCCCCCCC)C2 BBJQPKLGPMQWBU-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001840 cholesterol esters Chemical class 0.000 description 2
- UVZUFUGNHDDLRQ-LLHZKFLPSA-N cholesteryl benzoate Chemical compound O([C@@H]1CC2=CC[C@H]3[C@@H]4CC[C@@H]([C@]4(CC[C@@H]3[C@@]2(C)CC1)C)[C@H](C)CCCC(C)C)C(=O)C1=CC=CC=C1 UVZUFUGNHDDLRQ-LLHZKFLPSA-N 0.000 description 2
- BBJQPKLGPMQWBU-JADYGXMDSA-N cholesteryl palmitate Chemical compound C([C@@H]12)C[C@]3(C)[C@@H]([C@H](C)CCCC(C)C)CC[C@H]3[C@@H]1CC=C1[C@]2(C)CC[C@H](OC(=O)CCCCCCCCCCCCCCC)C1 BBJQPKLGPMQWBU-JADYGXMDSA-N 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
- 102000003914 Cholinesterases Human genes 0.000 description 1
- 108090000322 Cholinesterases Proteins 0.000 description 1
- QSJXEFYPDANLFS-UHFFFAOYSA-N Diacetyl Chemical group CC(=O)C(C)=O QSJXEFYPDANLFS-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000400624 Eucalyptus punctata Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000001647 Renal Insufficiency Diseases 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 229940048961 cholinesterase Drugs 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 229940093915 gynecological organic acid Drugs 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 201000006370 kidney failure Diseases 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 150000002923 oximes Chemical class 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
- C12Q1/46—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase involving cholinesterase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/521—Single-layer analytical elements
- G01N33/523—Single-layer analytical elements the element being adapted for a specific analyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/62—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving urea
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/17—Nitrogen containing
- Y10T436/171538—Urea or blood urea nitrogen
Definitions
- the diagnostic paper strip of this invention may be practically used as follows: the strip is dipped directly into blood obtained from a human or other animal and is kept for a period long enough to permit color development; after washing with water the color developed is compared with standard color chart. In the strip, no contamination and no red change are observed and the content or activity of the component to be determined can be rapidly and accurately determined.
Abstract
AN IMPROVED DIAGNOSTIC PAPER STRIP WHICH IS APPLICABLE TO WHOLE BLOOD SAMPLE, WHICH IS PREPARED BY DIPPING A DIAGNOSTIC PAPER STRIP INTO ORGANIC SOLVENT SOLUTION OF ONE OR MORE OF CHOLESTEROL, ROSIN AND THEIR ESTERS AND DRYING THE STRIP.
Description
United States Patent U.S. Cl. 23-230 12 Claims ABSTRACT OF THE DISCLOSURE An improved diagnostic paper strip which is applicable to whole blood sample, which is prepared by dipping a diagnostic paper strip into an organic solvent solution of one or more of cholesterol, rosin and their esters and drying the strip.
This invention relates to an improved diagnostic paper strip applicable to Whole blood samples of human of animals in testing of such blood for the determination of various disease states.
Human or animal blood is chemically composed of various components, for example inorganic components, proteins, glycose, organic acids, enzymes, vitamins and the like. The quantities of these components varies depending upon the disease state; and, accordingly, the quantitative determination of these various components is important for diagnosis of various diseases, and many blood determination methods have been utilized in the past. For example, precipitation method, flame analysis method, titration method, colorimetric determination method, enzyme reaction method, gas chromatography method, turibidimetric analysis method, electrophoresis analysis method, chromatography method, etc. (I. Kanai: Rinsho Kensa-ho Teiyo, ed. 21, Kanehara Shuppan K.K., Japan) have all been used in blood testing. However, clinical field results are often required rapidly and, therefore, more simple and rapid methods had been required particularly for use in the field and away from laboratories.
Recently, diagnostic paper strips, for example urea (ni trogen)-determining strip, serum cholinesterase-determining strip, calciumor magnesium-determining strip were found to meet such a demand, and it has been estimated that they can be conveniently used for the determination of content or activity of these components in blood serum by dipping them into serum and observing color developed thereon.
However, the method using these strips is still inadequate for rapid clinical diagnoses for the following reason; that is, these strips can be used to test only the serum which must be separated from whole blood sample by standing more than 30 minutes, since to dip the strip in while blood causes the strip to become so red due to hemoglobin in blood that it is impossible to observe color development by the component to be determined.
An object of this invention is to overcome the deficiencies of the prior art; and to provide an improved diagnostic paper strip which is applicable to whole blood samples. Another object of this invention is to provide a method for preparing the improved diagnostic paper strip. Further objects of this invention will be understood in detail in the following exemplary expanation.
We have now carried out examinations to fulfill the above objects and have found, as a result, the fact that if a diagnostic strip is treated with cholesterol, rosin or their esters, adsorption and permeation of red corpuscles on the strip can be prevented without preventing adsorption of the components to be determined and that color development by a component to be determined can be rapidly and ice accurately determined by dipping the so treated strip into a fresh whole blood sample.
This invention is found on such new findings and relates to an improved diagnostic paper strip which is applicable to whole blood sample, which is prepared by dipping a diagnostic paper strip into an organic solvent solution of one or more of cholesterol, rosin and their esters and drying the strip.
The diagnostic paper strip may be preferably prepared by dipping a diagnostic paper strip previously prepared, e.g. urea (nitrogen)-determining strip, serum cholinesterase-determining strip, calciumor magnesium-determining strip, into 1-10% by weight of an organic solvent solution. of one or more of cholesterol or its esters, e.g. cholesterol acetate or cholesterol benzoate, or rosin or its esters, e.g. gum rosin, ester gum (glycerin ester of rosin), methyl abietate, diethylene glycol ester of abietic acid, diethylene glycol ester of Z-hydro-abietic acid, etc. for 5-20 minutes and drying the strip at room temperature. In the above, the organic solvent is not critical as long as it dissolves the rosin or cholesterol or their derivatives, but suitable solvents are methyl alcohol, ethyl alcohol, carbon tetrachloride, benzene or the like. Thus obtained strip contains 0.52.5 mg. of cholesterol, rosin or their esters per square cm. of the strip.
The diagnostic paper strip of this invention may be practically used as follows: the strip is dipped directly into blood obtained from a human or other animal and is kept for a period long enough to permit color development; after washing with water the color developed is compared with standard color chart. In the strip, no contamination and no red change are observed and the content or activity of the component to be determined can be rapidly and accurately determined.
The invention will be more clearly illustrated by reference to the following detailed example, the scope of the invention not, however, being limited to the specific details of the examples wherein percents are weight by weight.
Blood was obtained from a healthy man and from two renal failure patients and sera were separated from a half of the blood samples. Urea content was determined by using untreated urea (nitrogen)-determining diagnostic paper strip and an improved urea (nitrogen)-determining diagnostic paper strip of this invention, both as disclosed in Example 1 below.
The results are shown in the following table.
RESULTS OF UREA (NITROGEN) CONTENT IN BLOOD Serum Whole blood Diacetyl mono- Treated oxime Untreated Untreated strip method strip strip of Ex. 1
Sample (mg/d1.) 1 (mg/d1.) (mg/d1.) (mg/d1.)
Healthy man 13 1O 10 Renal patient I 28 30 30 Renal patient IL... 42 40 4O EXAMPLE 1 Amberlite SA-Z type ion exchange resin paper was dipped into 0.05 N-hydrochloric acid solution containing 0.1% p-dimethylamino cinnarrraldehyde for 30' minutes. The resulting impregnated paper was dried at room temperature [urea (nitrogen)-determining strip, untreated].
The urea (nitrogen)-determining strip thus obtained was dipped into chloroform solution of cholesterol for minutes and dried at room temperature to give an improved diagnostic paper strip which is applicable to whole blood samples.
EXAMPLE 2 The same urea (nitrogen)-determining strip as obtained in the Example 1 was dipped into 4% ethyl alcohol solution of gum rosin for 10 minutes and dried at room temperature to give an improved diagnostic paper strip which is applicable to whole blood samples.
EXAMPLE 3 The urea (nitrogen)-determining strip obtained in the Example 1 was dipped into 2.5% ethyl alcohol solution of ester gum (glycerin ester of rosin) for 10 minutes and dried at room temperature to give an improved diagnostic paper strip which is applicable to whole blood samples.
EXAMPLE 4 A serum cholinesterase determining diagnostic paper,
strip, Acholest (trademark; produced by Osterreichische Stickstoffwerke A.G., Austria, and sold in Japan by Chugai Seiyaku Kabushi'ki Kaisha) was dipped into 3% carbon tetrachloride solution of cholesterol benzoate for 15 minutes and dried at room temperature to give an improved diagonistic paper strip which is applicable to whole blood samples.
The foregoing description of the specific embodiment will so fully reveal the general nature of the invention that others can, by applying current knowledge, readily modify such specific embodiment and/or adapt it fol various applications, without departing from the generic concept, and, therefore, such adaptations and modifications should and are intended to be comprehended within the meaning and range of equivalents.
We claim:
1. A method of preparing an improved diagnostic paper strip which is applicable to whole blood samples, consisting essentially of dipping a diagnostic paper strip into an organic solvent solution of one or more of cholesterol, rosin and their esters, and drying the strip.
2. The method claimed in claim 1, in which the diag- 4 nostic paper strip is urea (nitrogen)-determining strip, serum cholinesterase-determining strip or magnesiumor calcium-determining strip.
3. The method claimed in claim 1, in which the organic solvent to dissolve cholesterol, rosin or their esters is methyl alcohol, ethyl alcohol, chloroform, carbon tetrachloride or benzene.
4. The method claimed in claim 1, in which cholesterol ester is cholesterol acetate or cholesterol palmitate.
5. The method claimed in claim 1, in which rosin ester is gum rosin, ester gum, methyl abietate, diethylene glycol ester of abietic acid and diethylene glycol ester of 2-hydroabietic acid.
6. The method claimed in claim 1, in which the dipping time is 5-20 minutes and the concentration of said organic solution is 110%.
7. In diagnostic paper strip for blood testing comprising a paper strip impregnated with a testing chemical, the improvement comprising cholesterol, rosin, their esters or mixtures thereof impregnated in the strip.
8. An improved diagnostic paper strip in accordance with claim 7 wherein said cholesterol, rosin, their esters or mixtures thereof are present in said strip in an amount of about 0.5-2.5 mg. per sq. cm. of said strip.
9. The improved diagnostic paper strip claimed in claim 8, in which the diagnostic paper strip is selected from urea (nitrogen)-determining strip, serum cholinesterase-determining strip and magnesiumor calciumdetermining strip.
10. The improved diagnostic paper strip claimed in claim 8, in which said cholesterol ester is cholesterol acetate or cholesterol palmitate.
11. The improved diagnostic paper strip claimed in claim 8, in which said rosin ester is gum rosin, ester gum, methyl abietate, diethylene glycol ester of abietic acid or diethylene glycol ester of Z-hydroabietic acid.
12. A diagnostic method for determining content or activity of a component in blood which comprises dipping an improved diagnostic paper strip claimed in claim 8 into whole blood sample, keeping for a period long enough to take color development and comparing color developed with standard color chart.
References Cited FOREIGN PATENTS 1,037,155 7/1966 Great Britain 23253TP MORRIS O. WOLK, Primary Examiner R. M. REESE, Assist-ant Examiner U.S. Cl. X.R. 23-253; 1282
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2174268 | 1968-04-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US3598533A true US3598533A (en) | 1971-08-10 |
Family
ID=12063513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US818855A Expired - Lifetime US3598533A (en) | 1968-04-04 | 1969-04-01 | Diagnostic paper strip |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US3598533A (en) |
| CA (1) | CA920490A (en) |
| CH (1) | CH513402A (en) |
| DE (1) | DE1916132A1 (en) |
| FR (1) | FR2005516A1 (en) |
| GB (1) | GB1250093A (en) |
| SE (1) | SE360928B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4784158A (en) * | 1987-08-21 | 1988-11-15 | Okimoto Paul M | Vaginal testing applicator and method |
| US4806487A (en) * | 1987-05-29 | 1989-02-21 | Analytical Innovations, Inc. | Basic drug detection method |
| US4816415A (en) * | 1987-05-29 | 1989-03-28 | Analytical Innovations, Inc. | Cannabinoid detection method |
| WO2018222750A1 (en) * | 2017-05-30 | 2018-12-06 | Knonap Llc | Integrated devices for rapid detection of benzodiazepines or other drugs in solution |
-
1969
- 1969-03-18 SE SE03691/69A patent/SE360928B/xx unknown
- 1969-03-26 CA CA046946A patent/CA920490A/en not_active Expired
- 1969-03-28 DE DE19691916132 patent/DE1916132A1/en active Pending
- 1969-04-01 US US818855A patent/US3598533A/en not_active Expired - Lifetime
- 1969-04-02 FR FR6910008A patent/FR2005516A1/fr not_active Withdrawn
- 1969-04-03 GB GB1250093D patent/GB1250093A/en not_active Expired
- 1969-04-03 CH CH522069A patent/CH513402A/en not_active IP Right Cessation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4806487A (en) * | 1987-05-29 | 1989-02-21 | Analytical Innovations, Inc. | Basic drug detection method |
| US4816415A (en) * | 1987-05-29 | 1989-03-28 | Analytical Innovations, Inc. | Cannabinoid detection method |
| US4784158A (en) * | 1987-08-21 | 1988-11-15 | Okimoto Paul M | Vaginal testing applicator and method |
| WO2018222750A1 (en) * | 2017-05-30 | 2018-12-06 | Knonap Llc | Integrated devices for rapid detection of benzodiazepines or other drugs in solution |
Also Published As
| Publication number | Publication date |
|---|---|
| DE1916132A1 (en) | 1969-10-23 |
| CA920490A (en) | 1973-02-06 |
| FR2005516A1 (en) | 1969-12-12 |
| SE360928B (en) | 1973-10-08 |
| CH513402A (en) | 1971-09-30 |
| GB1250093A (en) | 1971-10-20 |
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