US3874852A - Reagent and method for determining leukocytes and hemoglobin in the blood - Google Patents
Reagent and method for determining leukocytes and hemoglobin in the blood Download PDFInfo
- Publication number
- US3874852A US3874852A US485931A US48593174A US3874852A US 3874852 A US3874852 A US 3874852A US 485931 A US485931 A US 485931A US 48593174 A US48593174 A US 48593174A US 3874852 A US3874852 A US 3874852A
- Authority
- US
- United States
- Prior art keywords
- ion
- quaternary ammonium
- reagent
- chain alkyl
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
- G01N33/721—Haemoglobin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
Abstract
Leukocytes and hemoglobin in the blood are determined in vitro with a reagent which comprises a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion in amounts sufficient to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for the determinations.
Claims (8)
1. A REAGENT FOR USE IN THE DETERMINATION OF LEUKOCYTES AND HEMOGLOBIN IN THE BLOOD, EHICH COMRPISES A FERRICYANIDE ION-FREE AQUEOUS SOLUTION CONTAINING A QUATERNARY AMMONIUM ION AND CYANIDE ION, SAID QUATERNARY AMMONIUM ION HAVING ATTACHED TO NITROGEN THREE SHORT CHAIN ALKYL GROUP AND ONE LONG CHAIN ALKYL GROUP, AND SAID IONS BEING PRESENT IN AMOUNTS SUFFSUFFICIENT TO STROMATOLYSE ERYTHROCYTE AND PLATELET CELLS AND CONVERT HEMOGOBLIN TO A CHROMOGEN FOR SAID DETERMINATIONS.
2. A reagent according to claim 1 wherein said quaternary ammonium ion is provided by a quaternary ammonium salt incorporated in said solution in a concentration in the range of about 0.5 to 10 percent by weight of said solution, and said cyanide ion is present in a concentration in the range of about 0.0006 to 0.005 molar.
3. A reagent according to claim 2 wherein said salt is a trimethyl tetradecyl ammonium halide, sulfate, phosphate, or nitrate, and said cyanide ion is provided by an alkali metal cyanide incorporated in said solution.
4. A reagent according to claim 1 wherein said short chain alkyl groups each have from 1 to 3 carbon atoms and said long chain alkyl group has from 10 to 14 carbon atoms.
5. A reagent according to claim 4 wherein said quaternary ammonium ion is provided by a quaternary ammonium salt incorporated in said solution in a concentration in the range of about 0.5 to 10 percent by weight of said solution, and said cyanide ion is provided by an alkali metal cyanide incorporated in said solution in a concentration in the range of about 0.0006 to 0.005 molar.
6. A reagent according to claim 5 wherein said salt is a halide, sulfate, phosphate, or nitrate.
7. A reagent according to claim 1 comprising a composition having the following ingredients and relative proportions:
8. In a method of determining leukocytes and hemoglobin in the blood wherein a reagent is reacted with a blood sample to stromatolyse erythrocyte and platelet cells and to convert hemoglobin to a chromogen for said determinations, the improvement which comprises employing as said reagent a ferricyanide ion-free aqueous solution containing a quaternary ammonium ion and cyanide ion, said quaternary ammonium ion having attached to nitrogen three short chain alkyl groups and one long chain alkyl group.
Priority Applications (16)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US485931A US3874852A (en) | 1974-07-05 | 1974-07-05 | Reagent and method for determining leukocytes and hemoglobin in the blood |
NL7415233.A NL164669B (en) | 1974-07-05 | 1974-11-22 | METHOD FOR PREPARING A REAGENT SUITABLE FOR DETERMINING LEUKOCYTES AND HEMOGLOBINE IN BLOOD. |
AU75725/74A AU482810B2 (en) | 1974-07-05 | 1974-11-25 | Reagent and method for determining leukocytes and hemoglobin inthe blood |
ZA00747574A ZA747574B (en) | 1974-07-05 | 1974-11-27 | Reagent and method for determining leukocytes and hemoglobin in the blood |
IL46137A IL46137A (en) | 1974-07-05 | 1974-11-27 | Reagent and method for determining leukocytes and hemoglobin in the blood |
IT54278/74A IT1029631B (en) | 1974-07-05 | 1974-11-28 | REGENT TO DETERMINE LEUCOCYTES AND HEMOGLOBIN IN THE BLOOD |
CH1585074A CH588078A5 (en) | 1974-07-05 | 1974-11-29 | |
JP754195A JPS616341B2 (en) | 1974-07-05 | 1974-12-28 | |
CA217,470A CA1024868A (en) | 1974-07-05 | 1975-01-07 | Reagent and method for determining leukocytes and hemoglobin in the blood |
BE6044886A BE824206A (en) | 1974-07-05 | 1975-01-08 | METHOD FOR PREPARING A REAGENT AND METHOD FOR DETERMINING LEUKOCYTE AND HEMOGLOBIN IN BLOOD |
FR7500452A FR2277344A1 (en) | 1974-07-05 | 1975-01-08 | REAGENT AND METHOD FOR DETERMINING LEUCOCYTES AND HEMOGLOBIN IN BLOOD |
SE7500245A SE415609B (en) | 1974-07-05 | 1975-01-10 | SET AND REAGENTS FOR DETERMINATION OF LEUKOCYTES AND HEMOGLOBIN IN BLOOD |
MX754018U MX4259E (en) | 1974-07-05 | 1975-01-15 | IMPROVED PROCEDURE FOR OBTAINING A COMPOSITION FOR Erythrocyte and Thrombocyte Stromatolysis in Blood |
ES433929A ES433929A1 (en) | 1974-07-05 | 1975-01-17 | Reagent and method for determining leukocytes and hemoglobin in the blood |
DE2501855A DE2501855C3 (en) | 1974-07-05 | 1975-01-17 | Reagent and method for the determination of leukocytes and hemoglobin in blood |
GB2184/75A GB1489303A (en) | 1974-07-05 | 1975-01-17 | Reagent and method for determining leukocytes and hemoglobin in the blood |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US485931A US3874852A (en) | 1974-07-05 | 1974-07-05 | Reagent and method for determining leukocytes and hemoglobin in the blood |
Publications (2)
Publication Number | Publication Date |
---|---|
US3874852A true US3874852A (en) | 1975-04-01 |
US3874852B1 US3874852B1 (en) | 1989-07-11 |
Family
ID=23929974
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US485931A Expired - Lifetime US3874852A (en) | 1974-07-05 | 1974-07-05 | Reagent and method for determining leukocytes and hemoglobin in the blood |
Country Status (15)
Country | Link |
---|---|
US (1) | US3874852A (en) |
JP (1) | JPS616341B2 (en) |
BE (1) | BE824206A (en) |
CA (1) | CA1024868A (en) |
CH (1) | CH588078A5 (en) |
DE (1) | DE2501855C3 (en) |
ES (1) | ES433929A1 (en) |
FR (1) | FR2277344A1 (en) |
GB (1) | GB1489303A (en) |
IL (1) | IL46137A (en) |
IT (1) | IT1029631B (en) |
MX (1) | MX4259E (en) |
NL (1) | NL164669B (en) |
SE (1) | SE415609B (en) |
ZA (1) | ZA747574B (en) |
Cited By (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4102810A (en) * | 1975-01-13 | 1978-07-25 | Coulter Electronics, Inc. | Stabilized hematological reagent solutions |
US4141856A (en) * | 1976-05-24 | 1979-02-27 | Dorwart Jr William V | Reference material for establishing anion concentrations in analytical chemistry tests |
US4185964A (en) * | 1977-02-08 | 1980-01-29 | Central Laboratories of Associated Maryland Pathologists, Ltd. | Lysing reagent |
US4219440A (en) * | 1979-06-06 | 1980-08-26 | Coulter Electronics, Inc. | Multiple analysis hematology reference control reagent and method of making the same |
EP0014929A1 (en) * | 1979-02-14 | 1980-09-03 | Roche Diagnostics GmbH | Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids |
US4286963A (en) * | 1979-11-23 | 1981-09-01 | Coulter Electronics, Inc. | Differential lymphoid-myeloid determination of leukocytes in whole blood |
DE3123669A1 (en) * | 1980-06-16 | 1982-04-22 | Coulter Electronics, Inc., 33010 Hialeah, Fla. | METHOD, MULTI-PURPOSE BLOOD DILUTION AND METHOD, REAGENT FOR THE DIFFERENTIAL DETERMINATION OF THE LYMPHOID-MYELOID POPULATION OF LEUKOCYTES |
US4455376A (en) * | 1979-09-17 | 1984-06-19 | R. J. Harvey Instrument Corp. | Photometric methods for counting the particulate components of blood |
WO1984002777A1 (en) * | 1983-01-03 | 1984-07-19 | Coulter Electronics | Method for the volumetric differential of leukocytes |
WO1984003771A1 (en) * | 1983-03-25 | 1984-09-27 | Coulter Electronics | Method and stromatolysing reagent for the volumetric determination of populations of leukocytes |
WO1984004969A1 (en) * | 1983-06-06 | 1984-12-20 | Coulter Electronics | Reagent for combined diluting and lysing whole blood |
EP0136058A2 (en) * | 1983-08-22 | 1985-04-03 | Ortho Diagnostic Systems Inc. | Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count |
US4521518A (en) * | 1980-06-16 | 1985-06-04 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US4526869A (en) * | 1980-09-24 | 1985-07-02 | Regents Of The University Of Minnesota | Method for quantitatively determining the concentration of hemoglobin in a biological sample |
US4543336A (en) * | 1982-02-12 | 1985-09-24 | Dragerwerk Aktiengesellschaft | Indicator for determining sulfur dioxide |
WO1985005640A1 (en) * | 1984-05-31 | 1985-12-19 | Coulter Electronics, Inc. | A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes |
US4617275A (en) * | 1983-09-29 | 1986-10-14 | Toa Medical Electronics Co., Ltd. | Reagent for blood analysis |
FR2582103A1 (en) * | 1985-03-19 | 1986-11-21 | Canavaggio Michel | METHOD FOR DETERMINING A BIOLOGICAL SUBSTANCE IN A SAMPLE |
US4725555A (en) * | 1986-10-22 | 1988-02-16 | Helena Laboratories Corporation | Zinc protoporphyrin test system |
US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
WO1988007187A1 (en) * | 1987-03-13 | 1988-09-22 | Coulter Electronics, Inc. | System for isolating and identifying leukocytes |
US4853338A (en) * | 1987-05-20 | 1989-08-01 | Technicon Instruments Corporation | Cyanide-free hemoglobin reagent |
US4962038A (en) * | 1980-06-16 | 1990-10-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US5008202A (en) * | 1988-11-29 | 1991-04-16 | Sequoia Turner Corporation | Blood diluent for red blood cell analysis |
US5045474A (en) * | 1987-05-28 | 1991-09-03 | Sequoia-Turner Corporation (Unipath) | Semi-automatic process for white cell differential count |
US5155044A (en) * | 1987-03-13 | 1992-10-13 | Coulter Electronics, Inc. | Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
US5510267A (en) * | 1989-05-15 | 1996-04-23 | Abbott Laboratories | Flow cytometry lytic agent and method enabling 5-part leukocyte differential count |
US5620852A (en) * | 1990-11-14 | 1997-04-15 | Hri Research, Inc. | Nucleic acid preparation methods |
US5654179A (en) * | 1990-11-14 | 1997-08-05 | Hri Research, Inc. | Nucleic acid preparation methods |
US5834315A (en) * | 1994-12-23 | 1998-11-10 | Coulter Corporation | Cyanide-free reagent and method for hemoglobin determination and leukocyte differentitation |
EP0878715A1 (en) * | 1997-05-13 | 1998-11-18 | Bayer Corporation | Cyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers |
US5958781A (en) * | 1994-07-14 | 1999-09-28 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US20040241769A1 (en) * | 1999-09-24 | 2004-12-02 | Crews Harold Richardson | Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells |
US20050106713A1 (en) * | 2003-09-03 | 2005-05-19 | Phan Brigitte C. | Personal diagnostic devices and related methods |
WO2014006638A1 (en) | 2012-07-03 | 2014-01-09 | Srivastava Ambar | Device and method for blood hemoglobin measurement without carboxyhemoglobin interference |
US9091677B2 (en) | 2010-08-09 | 2015-07-28 | Beckman Coulter, Inc. | Isotonic buffered composition and method that enables counting of cells |
US9523676B2 (en) | 2011-08-24 | 2016-12-20 | Eiken Kagaku Kabushiki Kaisha | Leukocyte measurement device and reagent kit |
CN113959911A (en) * | 2020-07-20 | 2022-01-21 | 深圳迈瑞生物医疗电子股份有限公司 | Detection method and reagent for resisting platelet aggregation interference and application thereof |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3024835A1 (en) * | 1980-07-01 | 1982-01-28 | Bayer Ag, 5090 Leverkusen | REAGENT FOR HEMOGLOBIN DETERMINATION |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3546131A (en) * | 1968-07-05 | 1970-12-08 | Uni Tech Chem Mfg Co | Stabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone |
US3607695A (en) * | 1969-02-14 | 1971-09-21 | Pfizer & Co C | Hemoglobinopathy controls |
US3663175A (en) * | 1970-10-05 | 1972-05-16 | Bio Dynamics Inc | Method of determining hemoglobin in blood |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE790042A (en) * | 1971-10-14 | 1973-04-13 | Coulter Electronics | METHOD FOR CLASSIFYING PARTICLES |
-
1974
- 1974-07-05 US US485931A patent/US3874852A/en not_active Expired - Lifetime
- 1974-11-22 NL NL7415233.A patent/NL164669B/en not_active IP Right Cessation
- 1974-11-27 ZA ZA00747574A patent/ZA747574B/en unknown
- 1974-11-27 IL IL46137A patent/IL46137A/en unknown
- 1974-11-28 IT IT54278/74A patent/IT1029631B/en active
- 1974-11-29 CH CH1585074A patent/CH588078A5/xx not_active IP Right Cessation
- 1974-12-28 JP JP754195A patent/JPS616341B2/ja not_active Expired
-
1975
- 1975-01-07 CA CA217,470A patent/CA1024868A/en not_active Expired
- 1975-01-08 BE BE6044886A patent/BE824206A/en not_active IP Right Cessation
- 1975-01-08 FR FR7500452A patent/FR2277344A1/en active Granted
- 1975-01-10 SE SE7500245A patent/SE415609B/en not_active IP Right Cessation
- 1975-01-15 MX MX754018U patent/MX4259E/en unknown
- 1975-01-17 DE DE2501855A patent/DE2501855C3/en not_active Expired
- 1975-01-17 GB GB2184/75A patent/GB1489303A/en not_active Expired
- 1975-01-17 ES ES433929A patent/ES433929A1/en not_active Expired
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3546131A (en) * | 1968-07-05 | 1970-12-08 | Uni Tech Chem Mfg Co | Stabilized cyanmethemoglobin reagent containing ferricyanide,cyanide and polyvinylpyrrolidone |
US3607695A (en) * | 1969-02-14 | 1971-09-21 | Pfizer & Co C | Hemoglobinopathy controls |
US3663175A (en) * | 1970-10-05 | 1972-05-16 | Bio Dynamics Inc | Method of determining hemoglobin in blood |
Cited By (59)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4102810A (en) * | 1975-01-13 | 1978-07-25 | Coulter Electronics, Inc. | Stabilized hematological reagent solutions |
US4141856A (en) * | 1976-05-24 | 1979-02-27 | Dorwart Jr William V | Reference material for establishing anion concentrations in analytical chemistry tests |
US4185964A (en) * | 1977-02-08 | 1980-01-29 | Central Laboratories of Associated Maryland Pathologists, Ltd. | Lysing reagent |
EP0014929A1 (en) * | 1979-02-14 | 1980-09-03 | Roche Diagnostics GmbH | Diagnostic means, process for its preparation and its use in determining leucocytes in body fluids |
US4219440A (en) * | 1979-06-06 | 1980-08-26 | Coulter Electronics, Inc. | Multiple analysis hematology reference control reagent and method of making the same |
US4455376A (en) * | 1979-09-17 | 1984-06-19 | R. J. Harvey Instrument Corp. | Photometric methods for counting the particulate components of blood |
US4286963A (en) * | 1979-11-23 | 1981-09-01 | Coulter Electronics, Inc. | Differential lymphoid-myeloid determination of leukocytes in whole blood |
GB2147999A (en) * | 1980-06-16 | 1985-05-22 | Coulter Electronics | Lysing reagent for differential determination of lymphoid-myeloid population of leukocytes |
DE3123669A1 (en) * | 1980-06-16 | 1982-04-22 | Coulter Electronics, Inc., 33010 Hialeah, Fla. | METHOD, MULTI-PURPOSE BLOOD DILUTION AND METHOD, REAGENT FOR THE DIFFERENTIAL DETERMINATION OF THE LYMPHOID-MYELOID POPULATION OF LEUKOCYTES |
US4962038A (en) * | 1980-06-16 | 1990-10-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
DE3153618C2 (en) * | 1980-06-16 | 1992-10-15 | Coulter Electronics, Inc., Hialeah, Fla., Us | |
US4346018A (en) * | 1980-06-16 | 1982-08-24 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US4521518A (en) * | 1980-06-16 | 1985-06-04 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US4526869A (en) * | 1980-09-24 | 1985-07-02 | Regents Of The University Of Minnesota | Method for quantitatively determining the concentration of hemoglobin in a biological sample |
US4543336A (en) * | 1982-02-12 | 1985-09-24 | Dragerwerk Aktiengesellschaft | Indicator for determining sulfur dioxide |
US4528274A (en) * | 1982-07-06 | 1985-07-09 | Coulter Electronics, Inc. | Multi-purpose blood diluent and lysing agent for differential determination of lymphoid-myeloid population of leukocytes |
US4485175A (en) * | 1983-01-03 | 1984-11-27 | Coulter Electronics, Inc. | Method for three-volume differential determination of lymphocyte, monocyte and granulocyte populations of leukocytes |
DE3390432C2 (en) * | 1983-01-03 | 1988-09-01 | Coulter Electronics | |
WO1984002777A1 (en) * | 1983-01-03 | 1984-07-19 | Coulter Electronics | Method for the volumetric differential of leukocytes |
WO1984003771A1 (en) * | 1983-03-25 | 1984-09-27 | Coulter Electronics | Method and stromatolysing reagent for the volumetric determination of populations of leukocytes |
US4529705A (en) * | 1983-06-06 | 1985-07-16 | Coulter Electronics, Inc. | Reagent for combined diluting and lysing whole blood |
WO1984004969A1 (en) * | 1983-06-06 | 1984-12-20 | Coulter Electronics | Reagent for combined diluting and lysing whole blood |
EP0136058A2 (en) * | 1983-08-22 | 1985-04-03 | Ortho Diagnostic Systems Inc. | Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count |
US4637986A (en) * | 1983-08-22 | 1987-01-20 | Ortho Diagnostic Systems, Inc. | Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part WBC count |
EP0136058A3 (en) * | 1983-08-22 | 1987-04-22 | Ortho Diagnostic Systems Inc. | Flow cytometry lysing reagent with leukoprotective agent for producing a 3-part wbc count |
US4656139A (en) * | 1983-09-29 | 1987-04-07 | Toa Medical Electronics Co., Ltd. | Method for preparing cells for blood analysis |
US4617275A (en) * | 1983-09-29 | 1986-10-14 | Toa Medical Electronics Co., Ltd. | Reagent for blood analysis |
WO1985005640A1 (en) * | 1984-05-31 | 1985-12-19 | Coulter Electronics, Inc. | A reagent system and method for identification, enumeration and examination of classes and subclasses of blood leukocytes |
FR2582103A1 (en) * | 1985-03-19 | 1986-11-21 | Canavaggio Michel | METHOD FOR DETERMINING A BIOLOGICAL SUBSTANCE IN A SAMPLE |
US4745071A (en) * | 1985-09-05 | 1988-05-17 | Sequoia-Turner Corporation | Method for the volumetric differentiation of blood cells types |
EP0268376A3 (en) * | 1986-10-22 | 1990-07-11 | Helena Laboratories Corporation | Blood sample modification and reagent compositions therefor |
EP0268376A2 (en) * | 1986-10-22 | 1988-05-25 | Helena Laboratories Corporation | Blood sample modification and reagent compositions therefor |
US4725555A (en) * | 1986-10-22 | 1988-02-16 | Helena Laboratories Corporation | Zinc protoporphyrin test system |
US5731206A (en) * | 1987-03-13 | 1998-03-24 | Coulter Electronics, Inc. | Method and reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples |
WO1988007187A1 (en) * | 1987-03-13 | 1988-09-22 | Coulter Electronics, Inc. | System for isolating and identifying leukocytes |
US5155044A (en) * | 1987-03-13 | 1992-10-13 | Coulter Electronics, Inc. | Lysing reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples |
US4853338A (en) * | 1987-05-20 | 1989-08-01 | Technicon Instruments Corporation | Cyanide-free hemoglobin reagent |
US5045474A (en) * | 1987-05-28 | 1991-09-03 | Sequoia-Turner Corporation (Unipath) | Semi-automatic process for white cell differential count |
US5008202A (en) * | 1988-11-29 | 1991-04-16 | Sequoia Turner Corporation | Blood diluent for red blood cell analysis |
US5510267A (en) * | 1989-05-15 | 1996-04-23 | Abbott Laboratories | Flow cytometry lytic agent and method enabling 5-part leukocyte differential count |
US5620852A (en) * | 1990-11-14 | 1997-04-15 | Hri Research, Inc. | Nucleic acid preparation methods |
US5654179A (en) * | 1990-11-14 | 1997-08-05 | Hri Research, Inc. | Nucleic acid preparation methods |
US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
US5958781A (en) * | 1994-07-14 | 1999-09-28 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US6740527B1 (en) | 1994-07-14 | 2004-05-25 | Abbott Laboratories | Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood |
US5834315A (en) * | 1994-12-23 | 1998-11-10 | Coulter Corporation | Cyanide-free reagent and method for hemoglobin determination and leukocyte differentitation |
EP0878715A1 (en) * | 1997-05-13 | 1998-11-18 | Bayer Corporation | Cyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers |
US5858794A (en) * | 1997-05-13 | 1999-01-12 | Bayer Corporation | Cyanide-containing hemoglobin reagent composition and method providing acceptable precision, accuracy and freedom from white cell interference on automated hematology analyzers |
US20040241769A1 (en) * | 1999-09-24 | 2004-12-02 | Crews Harold Richardson | Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells |
US20050106713A1 (en) * | 2003-09-03 | 2005-05-19 | Phan Brigitte C. | Personal diagnostic devices and related methods |
WO2005084534A1 (en) | 2003-09-03 | 2005-09-15 | Life Patch International, Inc. | Personal diagnostic devices and related methods |
US9133024B2 (en) | 2003-09-03 | 2015-09-15 | Brigitte Chau Phan | Personal diagnostic devices including related methods and systems |
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Also Published As
Publication number | Publication date |
---|---|
IL46137A0 (en) | 1975-02-10 |
US3874852B1 (en) | 1989-07-11 |
IT1029631B (en) | 1979-03-20 |
IL46137A (en) | 1977-02-28 |
NL7415233A (en) | 1976-01-07 |
AU7572574A (en) | 1976-05-27 |
SE415609B (en) | 1980-10-13 |
JPS616341B2 (en) | 1986-02-25 |
SE7500245L (en) | 1976-01-07 |
ES433929A1 (en) | 1976-11-16 |
MX4259E (en) | 1982-03-08 |
CA1024868A (en) | 1978-01-24 |
FR2277344B1 (en) | 1977-11-18 |
GB1489303A (en) | 1977-10-19 |
DE2501855B2 (en) | 1980-02-07 |
BE824206A (en) | 1975-07-08 |
DE2501855C3 (en) | 1980-10-16 |
FR2277344A1 (en) | 1976-01-30 |
CH588078A5 (en) | 1977-05-31 |
ZA747574B (en) | 1976-07-28 |
NL164669B (en) | 1980-08-15 |
JPS516788A (en) | 1976-01-20 |
DE2501855A1 (en) | 1976-01-22 |
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