US3897343A - Plasma separator-hydrostatic pressure type - Google Patents
Plasma separator-hydrostatic pressure type Download PDFInfo
- Publication number
- US3897343A US3897343A US446383A US44638374A US3897343A US 3897343 A US3897343 A US 3897343A US 446383 A US446383 A US 446383A US 44638374 A US44638374 A US 44638374A US 3897343 A US3897343 A US 3897343A
- Authority
- US
- United States
- Prior art keywords
- piston
- blood
- container
- phase
- sleeve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
Definitions
- ABSTRACT A blood collection and separator assembly of the type suitable for centrifuging to separate the plasma from the cellular phase of blood is disclosed.
- the assembly includes a collection container and a piston disposed therein for sealing off the plasma phase from the cellular phase after centrifuging is terminated.
- the piston has a specific gravity greater than the plasma but less than the cellular phase.
- Automatic compressible means is associated with the piston and is responsive to increased hydrostatic pressure caused by centrifugation so that the hydrostatic pressure reduces the diameter of the piston to provide a by-pass passage between the walls of the container and the piston so that the cellular phase can pass downwardly around the piston while the plasma phase passes upwardly therearound while the piston comes to rest at the plasmacellular interface, and when the hydrostatic force is terminated the compressible means expands to form a liquid tight seal to prevent subsequent mixing of the separated phases.
- This invention relates generally to plasma separator assemblies and particularly to a plasma separator having a piston and a compressible means associated therewith which automatically compresses when subjected to increased hydrostatic pressure caused by centrifugal force to provide a passageway between the piston and the interior of the collection container so that the cellular phase can pass downwardly around the piston in the container while the liquid or plasma phase passes upwardly therearound.
- My invention generally contemplates the provision of than the cellular phase so that the piston will move to the plasma interface and establish a liquid tight seal in the container after centrifuging is terminated.
- FIG. 1 is a sectional elevational view of the plasma separator assembly illustrating a pointed cannula penetrating the stoppered end of the container through which blood is introduced into the container prior to separation.
- FIG. 2 is a sectional elevational view after the cannula has been removed and the blood sample has been collected and separated into its phases during centrifugation and the centrifugal force has increased the hydrostatic pressure of the blood and has compressed the automatic compressible means associated with the piston thereby opening a passageway connecting the chamber regions above and below the piston.
- FIG. 3 is a view similar to FIG. 2 after the assembly has been centrifuged and the centrifugation has been stopped, the automatic compressible means having been released so as to be in sealing engagement with the container walls.
- FIG. 4 is a sectional elevational view of another form of the invention illustrating automatic compressible means mounted on the container wall and in a compressed condition, which provides for the passage of plasma upwardly in the container while the cellular or heavy phase passes downwardly to form an interface between the phases in the container during centrifuging of the assembly.
- FIG. 5 is a view similar to FIG. 3 after centrifuging has ceased which illustrates the automatic compressible means in sealing engagement with the piston to form a liquid tight seal which separates the plasma phase from the cellular phase in the assembly.
- FIG. 6 is a sectional elevational view of the piston after centrifuging has ceased illustrating another form of the automatic compressible means which can be used in conjunction with the embodiment of FIG. 1.
- FIG. 7 is an elevational sectional view of the piston of FIG. 6 when subjected to increased hydrostatic pressure generated by centrifuging the blood filled separator assembly.
- the separator assembly 10 comprises a tubular container 12 which is sealed at its open end by closure 14.
- Closure 14 is preferably made of rubber which is capable of being penetrated by cannula 15 so that blood can be transferred from a blood source into container 12 under aseptic conditions.
- the closure is made of elastomeric material and should be self-sealing so that when the cannula is removed there will be no loss of blood passing through the penetration portion of closure 14 as illustrated in FIG. 1.
- piston 16 Disposed in container 12 is piston 16 which is preferably made of one or more materials having an average specific gravity of approximately 1.06; for example, polystyrene may be used. Also, piston 16 should be made of a material which is inert to blood and to the resilient O-ring which is mounted in the annular groove 19 of piston 16. The parts forming the assembly may contain an anti-coagulant material. Piston 16 has mounted thereon compressible means such as O-ring 18 which automatically compresses when the assembly is subjected to increased hydrostatic pressure generated by centrifugal force. Compressible means is in the form of an O-ring 18 made of closed cell sponge rubber with a smooth outside surface (or equivalent) normally having an interference fit sealing the piston 16 relative to the container 12.
- compressible means is in the form of an O-ring 18 made of closed cell sponge rubber with a smooth outside surface (or equivalent) normally having an interference fit sealing the piston 16 relative to the container 12.
- O-ring 18 when increased hydrostatic pressure is generated by centrifugation, O-ring 18 is compressed unsealing the piston and thereby providing a passageway through which plasma and red cells may flow to form an interface.
- the piston has a specific gravity of approximately 1.06, which is less than the specific gravity of the cellular phase of blood but is heavier than the plasma phase of the blood.
- FIG. 2 shows conditions during centrifuging.
- O-ring 18 is illustrated in a compressed condition with piston 16 positioned at the plasma-cellular interface. Compression of O-ring 18 has created passageway 20 between the piston and the inside wall of the container so that the plasma can move upwardly through passage 20 and around piston 16 and red cells can move downwardly through passage 20. When centrifuging ceases, O-ring 18 will expand and re-establish the seal as illustrated in FIG. 3.
- FIGS. 1-3 includes sealing means responsive to or activated by changes in hydrostatic pressure.
- FIGS. 4 and 5 illustrate another form of compressible sealing means responsive to hydrostatic pressure which will automatically form a liquid tight seal to separate the plasma phase from the cellular phase when centrifuging of the assembly is terminated.
- FIG. 4 is similar to the embodiment illustrated in FIG. 2 in that assembly is in the process of being centrifuged and compressible means 17' is in a compressed condition to provide passage around piston 16' to permit piston 16 to move upwardly within the container along with the plasma phase while the cellular phase moves downwardly toward the bottom of the container.
- Container 12' is fitted with a closure 14' of the type described in the embodiment of FIG. 1.
- Piston 16 is made of material preferably having an average specific gravity of about 1.06 (for example, polystyrene) which is lighter than the cellular phase of blood but heavier than the plasma phase.
- Compressible means 17 automatically compresses in response to increased hydrostatic pressure generated by centrifugal force, and automatically expands when the centrifugal force is terminated.
- the compressible means is mounted in the form of a sleeve 17' on the interior surface of the container 12' at a point between the plasmacellular interface so that when piston 16' is permitted to move upwardly in the container piston 16 will come to rest at the plasma cellular interface. Then, when cen- ,4 trifuging is stopped, the piston 16' will be in sealing liquidtight engagement with the compressible means as illustrated in FIG. 5.
- Compressible sleeve 17' is preferably made of closed cell sponge rubber with a smooth outer skin (or equivalent) and is mounted on the inside surface of container 12.
- FIGS. 6 and 7 illustrate another form of compressible sealing means responsive to and actuated by changes in hydrostatic pressure.
- Piston 16 is formed of a suitable material having an average specific gravity greater than blood plasma but less than that of the cellular phase of blood.
- Compressible means 17" is in the form of a substantially non-porous elastomeric sleeve 18" expanded by slightly compressed air (or other suitable gas) and is secured on piston 16" around the upper and lower edges 22, 24 of sleeve 18".
- Piston 16" is formed having a body portion 25.
- a plurality of spaced annular grooves 26 are formed in body portion 25.
- Upper and lower flanges 29 and 27 of body portion 25 form shoulders 29 for mounting sleeve 18" thereon illustrated in FIG. 6.
- FIG. 6 In FIG.
- compressible means 17" is illustrated in a compressed condition with much of the air compressed into annular grooves 26 due to the increased hydrostatic generated by the centrifugal force so that resilient, compressible element 18" is in a compressed position to form passage 20 to permit the piston to move toward the plasma-cellular interface in a manner comparable to that illustrated in FIG. 2.
- the compressible element 17 is preferably made of a closed cell sponge rubber with a smooth outer skin and is cemented or otherwise fixed to the inside wall of container 12 as illustrated in FIGS. 4 and 5.
- the piston in the embodiments illustrated herein can be inserted into the assembly prior to use as described or it can be inserted after the blood has been collected by simply removing closure 14 and manually inserting the appropriate piston in the corresponding type container and then centrifuging the assembly to permit the piston to move downwardly in the container to the plasma cellular phase interface rather than upwardly as described.
- the pistons are not put into the containers until after the blood has been centrifuged into the light phase and the heavy phase, then these devices can be used for serum, as distinguished from plasma. Then the containers would be centrifuged a second time to move the pistons to the serum red cell interface.
- tube 12 can be formed having openings at each end with the piston positioned at one end so that with blood being collected through the closure member at the opposite end the piston will move downwardly in the container rather than upwardly.
- the blood collection tube 12 which is fitted with closure 14 is preferably evacuated so that when cannula l5 penetrates the closure 14 blood will fill container 12 automatically.
- the separator assembly of the invention herein may be constructed so as to be suitable for use with blood collecting assemblies described in US. Pat. Nos. 3,460,641; 3,469,572 and 3,494,352. It should be understood that when the blood is being collected where the piston 16, 16' or 16" is in the bottom of the container then the blood being collected will be anticoag'ulated so that a clot will not form which might cause a malfunction of the piston.
- a piston adapted for use for separating the light phase of blood from the cellular or heavy phase of anticoagulant treated blood disposed in a separator assembly including a blood collection container comprising:
- a piston having an average specific gravity heavier than the light phase of blood but lighter than the heavy phase so that when the blood is separated into its component phases the piston will migrate to the light phase-heavy phase interface; said piston comprising;
- a body portion which is generally cylindrical and has a diameter less than the internal diameter of the blood collection container; a substantially nonporous, flexible sleeve member mounted around said body portion and sealed around the top and bottom ends thereof, thereby forming an annular space therebetween, said space having a gas therein, said gas having a pressure greater than atmospheric pressure and selected so as to expand the flexible barrier means to form a liquid-tight seal when mounted within the container and said gas being compressible when subjected to increased hydrostatic force so that the flexible barrier means will move away from the inner walls of the container to break the seal and pennit the piston to move toward the cellular-plasma interface when centrifuged.
- piston of claim 1 wherein the piston is formed of polystyrene having a specific gravity of substantially 1.06.
- a blood separator assembly capable of separating anti-coagulant treated blood into its component parts of light phase and heavy phase comprising:
- a container having at least one open end which is adapted to receive blood for subsequent separation into a light phase and a heavy phase;
- closure sealing the open end of the container, the closure being formed of a self-sealing, elastomeric material which is penetrable by a cannula through which blood to be separated is conducted into the container;
- said piston having a body portion which is generally cylindrical and has a diameter less than the internal diameter of the blood collection container;
- a compressible sleeve mounted on the interior walls of the container, so as to form a seal with said piston when said piston is positioned within said sleeve and said sleeve is uncompressed, the sleeve being made of closed cell elastomeric material having a smooth surface and said sleeve is responsive to hydrostatic forces whereby when said sleeve is compressed by subjugation to hydrostatic forces, said piston no longer forms a seal with said sleeve, thereby providing a passage for the light phase and heavy phase of the blood to move past the piston.
Abstract
A blood collection and separator assembly of the type suitable for centrifuging to separate the plasma from the cellular phase of blood is disclosed. The assembly includes a collection container and a piston disposed therein for sealing off the plasma phase from the cellular phase after centrifuging is terminated. The piston has a specific gravity greater than the plasma but less than the cellular phase. Automatic compressible means is associated with the piston and is responsive to increased hydrostatic pressure caused by centrifugation so that the hydrostatic pressure reduces the diameter of the piston to provide a by-pass passage between the walls of the container and the piston so that the cellular phase can pass downwardly around the piston while the plasma phase passes upwardly therearound while the piston comes to rest at the plasma-cellular interface, and when the hydrostatic force is terminated the compressible means expands to form a liquid tight seal to prevent subsequent mixing of the separated phases.
Description
United States Patent Ayres Primary Examiner-Charles N. Hart Assistant ExaminerRobert G. Mukai Attorney, Agent, or Firm-Kane, Dalsimer, Kane, Sullivan and Kurucz 1 July 29, 1975 [57] ABSTRACT A blood collection and separator assembly of the type suitable for centrifuging to separate the plasma from the cellular phase of blood is disclosed. The assembly includes a collection container and a piston disposed therein for sealing off the plasma phase from the cellular phase after centrifuging is terminated. The piston has a specific gravity greater than the plasma but less than the cellular phase. Automatic compressible means is associated with the piston and is responsive to increased hydrostatic pressure caused by centrifugation so that the hydrostatic pressure reduces the diameter of the piston to provide a by-pass passage between the walls of the container and the piston so that the cellular phase can pass downwardly around the piston while the plasma phase passes upwardly therearound while the piston comes to rest at the plasmacellular interface, and when the hydrostatic force is terminated the compressible means expands to form a liquid tight seal to prevent subsequent mixing of the separated phases.
5 Claims, 7 Drawing Figures PATENTED JUL 2 9 i975 SHEET PLASMA SEPARATOR-HYDROSTATIC PRESSURE TYPE BACKGROUND OF THE INVENTION This invention relates generally to plasma separator assemblies and particularly to a plasma separator having a piston and a compressible means associated therewith which automatically compresses when subjected to increased hydrostatic pressure caused by centrifugal force to provide a passageway between the piston and the interior of the collection container so that the cellular phase can pass downwardly around the piston in the container while the liquid or plasma phase passes upwardly therearound.
DESCRIPTION OF THE PRIOR ART It is known to separate blood into its component parts by centrifugation, for example, the assembly disclosed in U.S. Pat. No. 2,460,641. However, this particular assembly does not employ a means for sealing the separated plasma or serum phase from the cellular phase.
It is also known to provide assemblies for manually separating the plasma or serum phase from the cellular phase, for example, as disclosed in U.S. Pat. Nos. 3,586,064; 3,661,265; 3,355,098; 3,481,477; 3,512,940 and 3,693,804. In all of these devices serum is collected in a blood collection container and means are provided for separating the plasma phase from the cellular phase employing filters, valves, transfer tubes or the like.
It is also known to provide assemblies for the sealed separation of blood in which a piston is actuated by centrifugal force such as disclosed in U.S. Pat. Nos. 3,508,653 and 3,779,383. These devices use either a distortable piston made of a resilient material or valve means associated with the piston to affect a sealed separation after centrifugation.
SUMMARY OF THE INVENTION It is an object of the present invention to provide a plasma separator assembly having a piston and a compressible means which automatically seals off the plasma phase from the cellular phase after centrifuging is terminated. It is another object of the invention to provide a piston having means associated therewith which is compressible to provide a path around the piston to permit the passage of the plasma phase to pass upwardly therearound while permitting the cellular phase to pass downwardly therearound and which will automatically seal the piston in the container when centrifuging is terminated.
It is an object of the invention to provide a plasma separator assembly which is economical to manufacture and can be used in conjunction with standard blood collecting equipment.
My invention generally contemplates the provision of than the cellular phase so that the piston will move to the plasma interface and establish a liquid tight seal in the container after centrifuging is terminated.
DESCRIPTION OF THE DRAWINGS For a better understanding of the invention, reference is bad to the drawings which illustrate the preferred embodiments of the invention herein.
FIG. 1 is a sectional elevational view of the plasma separator assembly illustrating a pointed cannula penetrating the stoppered end of the container through which blood is introduced into the container prior to separation.
FIG. 2 is a sectional elevational view after the cannula has been removed and the blood sample has been collected and separated into its phases during centrifugation and the centrifugal force has increased the hydrostatic pressure of the blood and has compressed the automatic compressible means associated with the piston thereby opening a passageway connecting the chamber regions above and below the piston.
FIG. 3 is a view similar to FIG. 2 after the assembly has been centrifuged and the centrifugation has been stopped, the automatic compressible means having been released so as to be in sealing engagement with the container walls.
FIG. 4 is a sectional elevational view of another form of the invention illustrating automatic compressible means mounted on the container wall and in a compressed condition, which provides for the passage of plasma upwardly in the container while the cellular or heavy phase passes downwardly to form an interface between the phases in the container during centrifuging of the assembly.
FIG. 5 is a view similar to FIG. 3 after centrifuging has ceased which illustrates the automatic compressible means in sealing engagement with the piston to form a liquid tight seal which separates the plasma phase from the cellular phase in the assembly.
FIG. 6 is a sectional elevational view of the piston after centrifuging has ceased illustrating another form of the automatic compressible means which can be used in conjunction with the embodiment of FIG. 1.
FIG. 7 is an elevational sectional view of the piston of FIG. 6 when subjected to increased hydrostatic pressure generated by centrifuging the blood filled separator assembly.
DESCRIPTION OF THE PREFERRED EMBODIMENTS For a better understanding of the invention here is a description and the drawings of illustrative embodiments, particularly as shown in FIGS. 1-3.
In FIG. 1 the separator assembly 10 comprises a tubular container 12 which is sealed at its open end by closure 14. Closure 14 is preferably made of rubber which is capable of being penetrated by cannula 15 so that blood can be transferred from a blood source into container 12 under aseptic conditions. The closure is made of elastomeric material and should be self-sealing so that when the cannula is removed there will be no loss of blood passing through the penetration portion of closure 14 as illustrated in FIG. 1.
Disposed in container 12 is piston 16 which is preferably made of one or more materials having an average specific gravity of approximately 1.06; for example, polystyrene may be used. Also, piston 16 should be made of a material which is inert to blood and to the resilient O-ring which is mounted in the annular groove 19 of piston 16. The parts forming the assembly may contain an anti-coagulant material. Piston 16 has mounted thereon compressible means such as O-ring 18 which automatically compresses when the assembly is subjected to increased hydrostatic pressure generated by centrifugal force. Compressible means is in the form of an O-ring 18 made of closed cell sponge rubber with a smooth outside surface (or equivalent) normally having an interference fit sealing the piston 16 relative to the container 12. However, when increased hydrostatic pressure is generated by centrifugation, O-ring 18 is compressed unsealing the piston and thereby providing a passageway through which plasma and red cells may flow to form an interface. The piston has a specific gravity of approximately 1.06, which is less than the specific gravity of the cellular phase of blood but is heavier than the plasma phase of the blood. When blood is collected in container 12, as illustrated in FIG. 1, and the container is centrifuged, as illustrated in FIG. 2, the cellular phase will pass downwardly into the bottom portion of container 12 while piston 16 moves upwardly to finally come to rest at the plasma-cellular interface. When centrifuging is terminated the hydrostatic pressure drops and the O-ring l8 expands to form a liquid tight seal, as illustrated in FIG. 3.
FIG. 2 shows conditions during centrifuging. O-ring 18 is illustrated in a compressed condition with piston 16 positioned at the plasma-cellular interface. Compression of O-ring 18 has created passageway 20 between the piston and the inside wall of the container so that the plasma can move upwardly through passage 20 and around piston 16 and red cells can move downwardly through passage 20. When centrifuging ceases, O-ring 18 will expand and re-establish the seal as illustrated in FIG. 3.
Thus, the invention embodiment shown in FIGS. 1-3 includes sealing means responsive to or activated by changes in hydrostatic pressure.
FIGS. 4 and 5 illustrate another form of compressible sealing means responsive to hydrostatic pressure which will automatically form a liquid tight seal to separate the plasma phase from the cellular phase when centrifuging of the assembly is terminated.
FIG. 4 is similar to the embodiment illustrated in FIG. 2 in that assembly is in the process of being centrifuged and compressible means 17' is in a compressed condition to provide passage around piston 16' to permit piston 16 to move upwardly within the container along with the plasma phase while the cellular phase moves downwardly toward the bottom of the container. Container 12' is fitted with a closure 14' of the type described in the embodiment of FIG. 1. Piston 16 is made of material preferably having an average specific gravity of about 1.06 (for example, polystyrene) which is lighter than the cellular phase of blood but heavier than the plasma phase. Compressible means 17 automatically compresses in response to increased hydrostatic pressure generated by centrifugal force, and automatically expands when the centrifugal force is terminated. The compressible means is mounted in the form of a sleeve 17' on the interior surface of the container 12' at a point between the plasmacellular interface so that when piston 16' is permitted to move upwardly in the container piston 16 will come to rest at the plasma cellular interface. Then, when cen- ,4 trifuging is stopped, the piston 16' will be in sealing liquidtight engagement with the compressible means as illustrated in FIG. 5. Compressible sleeve 17' is preferably made of closed cell sponge rubber with a smooth outer skin (or equivalent) and is mounted on the inside surface of container 12.
FIGS. 6 and 7 illustrate another form of compressible sealing means responsive to and actuated by changes in hydrostatic pressure. Piston 16 is formed of a suitable material having an average specific gravity greater than blood plasma but less than that of the cellular phase of blood.
In the embodiment of FIGS. 4 and 5 the compressible element 17 is preferably made of a closed cell sponge rubber with a smooth outer skin and is cemented or otherwise fixed to the inside wall of container 12 as illustrated in FIGS. 4 and 5. It should be understood that the piston in the embodiments illustrated herein can be inserted into the assembly prior to use as described or it can be inserted after the blood has been collected by simply removing closure 14 and manually inserting the appropriate piston in the corresponding type container and then centrifuging the assembly to permit the piston to move downwardly in the container to the plasma cellular phase interface rather than upwardly as described. Also, if the pistons are not put into the containers until after the blood has been centrifuged into the light phase and the heavy phase, then these devices can be used for serum, as distinguished from plasma. Then the containers would be centrifuged a second time to move the pistons to the serum red cell interface.
It is also apparent that tube 12 can be formed having openings at each end with the piston positioned at one end so that with blood being collected through the closure member at the opposite end the piston will move downwardly in the container rather than upwardly.
When operating the separator assembly as set forth in the preferred embodiments the blood collection tube 12 which is fitted with closure 14 is preferably evacuated so that when cannula l5 penetrates the closure 14 blood will fill container 12 automatically. It is also contemplated that the separator assembly of the invention herein may be constructed so as to be suitable for use with blood collecting assemblies described in US. Pat. Nos. 3,460,641; 3,469,572 and 3,494,352. It should be understood that when the blood is being collected where the piston 16, 16' or 16" is in the bottom of the container then the blood being collected will be anticoag'ulated so that a clot will not form which might cause a malfunction of the piston.
After the blood has been collected in container 12, assembly is ready for centrifuging. The compressible means, such as the O-ring 18 or the compressible sleeve 17' or the inflated annular ring 18" will be compressed when subjected to increased hydrostatic pressure resulting from centrifugal force. Centrifugation will also cause the serum to pass upwardly in the container around the piston 16 through passage 20 and at the same time for the cellular phase to move toward the bottom of the container while the piston moves towards the cellular-plasma interface. When the blood has been separated the piston will lie at the plasma-cellular interface and when centrifuging ceases the compressible means 18 will re-expand to form a liquid tight seal as shown in FIG. 3. Thus, an assembly is provided in which blood can be controlled, centrifuged, separated into its plasma and cellular phases and shipped through the mails for further analytical determinations without the plasma mixing with the cellular phase even though the assembly is inverted and handled roughly.
While variations of the invention herein may be had, the objectives of the invention have been illustrated and described, it is contemplated that changes in design can be made without departing from the spirit of the invention described herein.
What is claimed is:
1. A piston adapted for use for separating the light phase of blood from the cellular or heavy phase of anticoagulant treated blood disposed in a separator assembly including a blood collection container comprising:
a piston having an average specific gravity heavier than the light phase of blood but lighter than the heavy phase so that when the blood is separated into its component phases the piston will migrate to the light phase-heavy phase interface; said piston comprising;
a body portion which is generally cylindrical and has a diameter less than the internal diameter of the blood collection container; a substantially nonporous, flexible sleeve member mounted around said body portion and sealed around the top and bottom ends thereof, thereby forming an annular space therebetween, said space having a gas therein, said gas having a pressure greater than atmospheric pressure and selected so as to expand the flexible barrier means to form a liquid-tight seal when mounted within the container and said gas being compressible when subjected to increased hydrostatic force so that the flexible barrier means will move away from the inner walls of the container to break the seal and pennit the piston to move toward the cellular-plasma interface when centrifuged.
2. The piston of claim 1 wherein the piston is formed of polystyrene having a specific gravity of substantially 1.06.
3. A blood separator assembly capable of separating anti-coagulant treated blood into its component parts of light phase and heavy phase comprising:
a container having at least one open end which is adapted to receive blood for subsequent separation into a light phase and a heavy phase;
a closure sealing the open end of the container, the closure being formed of a self-sealing, elastomeric material which is penetrable by a cannula through which blood to be separated is conducted into the container;
a piston having an average specific gravity lighter than the heavy phase but heavier than the light phase;
said piston having a body portion which is generally cylindrical and has a diameter less than the internal diameter of the blood collection container;
a compressible sleeve mounted on the interior walls of the container, so as to form a seal with said piston when said piston is positioned within said sleeve and said sleeve is uncompressed, the sleeve being made of closed cell elastomeric material having a smooth surface and said sleeve is responsive to hydrostatic forces whereby when said sleeve is compressed by subjugation to hydrostatic forces, said piston no longer forms a seal with said sleeve, thereby providing a passage for the light phase and heavy phase of the blood to move past the piston.
4. The assembly of claim 3 wherein the piston is formed of polystyrene having a specific gravity of substantially 1.06.
5. The assembly of claim 3 wherein said compressible sleeve is made of closed-cell elastomeric sponge material having a smooth surface.
Claims (5)
1. A PISTON ADAPTED FOR USE FOR SEPARATING THE LIGHT PHASE OF BLOOD FROM THE CELLULAR OR HEAVY PHASE OF ANTI-COAGULANT TREATED BLOOD DISPOSED IN A SEPARATOR ASSEMBLY INCLUDING A BLOOD COLLECTION CONTAINER COMPRISING: A PISTON HAVING AN AVERAGE SPECIFIC GRAVITY HEAVIER THAN THE LIGHT PHASE OF BLOOD BUT LIGHTER THAN THE HEAVY PHASE SO THAT WHEN THE BLOOD IS SEPARATED INTO ITS COMPONENT PHASES THE PISTON WILL MIGRATE TO THE LIGHT PHASE-HEAVY PHASE INTERFACE SAID PISTON COMPRISING, A BODYPORTION WHICH IS GENERALLY CYLINDRICAL AND HAS A DIAMETER LESS THAN THE INTERNAL DIAMETER OF THE BLOOD COLLECTION CONTAINER A SUBSTANTIALLY NON-POROUS FLEXIBLE SLEEVE MEMBER MOUNTED AROUND SAID BODY PORTION AND SEALED AROUND THE TOP AND BOTTOM ENDS THEREOF THEREBY FORMING AN ANNULAR SPACE THEREBETWEEN SAID SPACE HAVING A GAS THEREIN SAID GAS HAVING A PRESSURE GREATER THAN ATMOSPHERIC PRESSURE AND SELECTED SO AS TO EXPAND THE FLEXIBLE BARRIER MEANS TO FORM A LIQUID-TIGHT SEAL WHEN MOUNTED WITHIN THE CONTAINER ANDD SAID GAS BEING COMPRESSIBLE WHEN SUBJECTING TO INCREASED HYDROSTATIC FORCE SO THAT THE FLEXIBLE BARRIER MEANS WILL MOVE AWAY FROM THE INNER WALLS OF THE CONTAINER TO BREAK THE SEAL AND PERMIT
2. The piston of claim 1 wherein the piston is formed of polystyrene having a specific gravity of substantially 1.06.
3. A blood separator assembly capable of separating anti-coagulant treated blood into its component parts of light phase and heavy phase comprising: a container having at least one open end which is adapted to receive blood for subsequent separation into a light phase and a heavy phase; a closure sealing the open end of the container, the closure being formed of a self-sealing, elastomeric material which is penetrable by a cannula through which blood to be separated is conducted into the container; a piston having an average specific gravity lighter than the heavy phase but heavier than the light phase; said piston having a body portion which is generally cylindrical and has a diameter less than the internal diameter of the blood collection container; a compressible sleeve mounted on the interior walls of the container, so as to form a seal with said piston when said piston is positioned within said sleeve and said sleeve is uncompressed, the sleeve being made of closed cell elastomeric material having a smooth surface and said sleeve is responsive to hydrostatic forces whereby when said sleeve is compressed by subjugation to hydrostatic forces, said piston no longer forms a seal with said sleeve, thereby providing a passage for the light phase and heavy phase of the blood to move past the piston.
4. The assembly of claim 3 wherein the piston is formed of polystyrene having a specific gravity of substantially 1.06.
5. The assembly of claim 3 wherein said compressible sleeve is made of closed-cell elastomeric sponge material having a smooth surface.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US446383A US3897343A (en) | 1974-02-27 | 1974-02-27 | Plasma separator-hydrostatic pressure type |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US446383A US3897343A (en) | 1974-02-27 | 1974-02-27 | Plasma separator-hydrostatic pressure type |
Publications (1)
Publication Number | Publication Date |
---|---|
US3897343A true US3897343A (en) | 1975-07-29 |
Family
ID=23772384
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US446383A Expired - Lifetime US3897343A (en) | 1974-02-27 | 1974-02-27 | Plasma separator-hydrostatic pressure type |
Country Status (1)
Country | Link |
---|---|
US (1) | US3897343A (en) |
Cited By (74)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4152270A (en) * | 1976-05-06 | 1979-05-01 | Sherwood Medical Industries Inc. | Phase separation device |
US4169060A (en) * | 1977-10-25 | 1979-09-25 | Eastman Kodak Company | Blood-collecting and serum-dispensing device |
US4811866A (en) * | 1987-01-02 | 1989-03-14 | Helena Laboratories Corporation | Method and apparatus for dispensing liquids |
US4818386A (en) * | 1987-10-08 | 1989-04-04 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4877520A (en) * | 1987-10-08 | 1989-10-31 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4892714A (en) * | 1988-10-13 | 1990-01-09 | Microscale Organic Laboratory Corporation | Recrystallization apparatus |
US5248480A (en) * | 1992-05-28 | 1993-09-28 | Diasys Corporation | Apparatus for drawing fluid sample and components thereof |
US5251474A (en) * | 1992-01-16 | 1993-10-12 | Wardlaw Stephen C | Centrifuged material layer measurement in an evacuated tube |
US5266199A (en) * | 1990-11-20 | 1993-11-30 | Nigata Chemicals And Plastics Co., Ltd. | Serum separating apparatus |
US5393674A (en) * | 1990-12-31 | 1995-02-28 | Levine Robert A | Constitutent layer harvesting from a centrifuged sample in a tube |
US5393494A (en) * | 1992-05-28 | 1995-02-28 | Diasys Corporation | Apparatus for drawing fluid sample, components thereof, and slide assembly for use therewith |
WO1996018897A1 (en) * | 1994-12-13 | 1996-06-20 | Coleman Charles M | Separator float for blood collection tubes |
EP0753741A1 (en) * | 1995-01-30 | 1997-01-15 | Niigata Engineering Co., Ltd. | Component separation member and component separator equipped with said member |
US5632895A (en) * | 1993-08-13 | 1997-05-27 | Nigata Engineering Co., Ltd. | Serum separating device and apparatus for serum separation |
WO1998005426A2 (en) * | 1996-08-02 | 1998-02-12 | C.A. Greiner & Söhne Gesellschaft Mbh | Sealing device, separating device and collecting receptacle for a collector device |
US5736033A (en) * | 1995-12-13 | 1998-04-07 | Coleman; Charles M. | Separator float for blood collection tubes with water swellable material |
WO1998051411A2 (en) * | 1997-05-12 | 1998-11-19 | C.A. Greiner & Söhne Gesellschaft Mbh | Separating in a centrifugable container and separating method |
EP1005909A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Centrifuge tube with round separation element, liner and cap |
EP1005910A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Centrifuge tube with cylindrically symmetric separation element, liner and cap |
EP1106252A2 (en) * | 1999-12-06 | 2001-06-13 | Becton, Dickinson and Company | Device and method for collecting, preparation and stabilizing a sample |
EP1106251A2 (en) * | 1999-12-06 | 2001-06-13 | Becton, Dickinson and Company | Device and method for separating components of a fluid sample |
US20020094305A1 (en) * | 1999-12-06 | 2002-07-18 | Dicesare Paul C. | Device and method for separating components of a fluid sample |
US6479298B1 (en) * | 1998-12-05 | 2002-11-12 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US20040166029A1 (en) * | 1999-12-03 | 2004-08-26 | Losada Robert J. | Device for separating components of a fluid sample |
US20040182795A1 (en) * | 2003-03-21 | 2004-09-23 | Randel Dorian | Apparatus and method for concentration of plasma from whole blood |
US20040182788A1 (en) * | 2003-03-21 | 2004-09-23 | Randel Dorian | Plasma concentrate apparatus and method |
US20040217046A1 (en) * | 2001-03-30 | 2004-11-04 | Franz Konrad | Holding device, particularly for body fluids, comprising a separating device, and a separating device therefor |
US7074577B2 (en) | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US20060273049A1 (en) * | 2002-05-24 | 2006-12-07 | Leach Michael D | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
US20060278588A1 (en) * | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
US20070003449A1 (en) * | 2005-06-10 | 2007-01-04 | Mehdi Hatamian | Valve for facilitating and maintaining fluid separation |
US7179391B2 (en) | 2002-05-24 | 2007-02-20 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
US20070075016A1 (en) * | 2005-08-23 | 2007-04-05 | Biomet Manufacturing Corp. | Method and apparatus for collecting biological materials |
US20070102344A1 (en) * | 2005-10-04 | 2007-05-10 | Franz Konrad | Separating device, holding device and method for separation |
US20070208321A1 (en) * | 2005-08-23 | 2007-09-06 | Biomet Manufacturing Corp. | Method And Apparatus For Collecting Biological Materials |
US20080011684A1 (en) * | 2005-02-07 | 2008-01-17 | Dorian Randel E | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US7374678B2 (en) | 2002-05-24 | 2008-05-20 | Biomet Biologics, Inc. | Apparatus and method for separating and concentrating fluids containing multiple components |
US20080223815A1 (en) * | 2004-01-23 | 2008-09-18 | Franz Konrad | Method for the Assembly of a Cap with a Receptacle |
US20090090671A1 (en) * | 2007-10-05 | 2009-04-09 | Mady Attila | Apparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae |
US20090236297A1 (en) * | 2005-02-07 | 2009-09-24 | Hanuman, Llc | Plasma Concentrator Device |
US7694828B2 (en) | 2005-04-27 | 2010-04-13 | Biomet Manufacturing Corp. | Method and apparatus for producing autologous clotting components |
US7708152B2 (en) | 2005-02-07 | 2010-05-04 | Hanuman Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
US20100140182A1 (en) * | 2008-12-04 | 2010-06-10 | Chapman John R | Apparatus and method for separating and isolating components of a biological fluid |
US20100160135A1 (en) * | 2008-07-21 | 2010-06-24 | Becton, Dickinson And Company | Density Phase Separation Device |
US7806276B2 (en) | 2007-04-12 | 2010-10-05 | Hanuman, Llc | Buoy suspension fractionation system |
US7824559B2 (en) | 2005-02-07 | 2010-11-02 | Hanumann, LLC | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US20100288694A1 (en) * | 2009-05-15 | 2010-11-18 | Becton, Dickinson And Company | Density Phase Separation Device |
US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US8012077B2 (en) | 2008-05-23 | 2011-09-06 | Biomet Biologics, Llc | Blood separating device |
US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
US8337711B2 (en) | 2008-02-29 | 2012-12-25 | Biomet Biologics, Llc | System and process for separating a material |
US8394342B2 (en) | 2008-07-21 | 2013-03-12 | Becton, Dickinson And Company | Density phase separation device |
US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
US9333445B2 (en) | 2008-07-21 | 2016-05-10 | Becton, Dickinson And Company | Density phase separation device |
US9393575B2 (en) | 2000-04-28 | 2016-07-19 | Harvest Technologies Corporation | Blood components separator disk |
US9550028B2 (en) | 2014-05-06 | 2017-01-24 | Biomet Biologics, LLC. | Single step desiccating bead-in-syringe concentrating device |
US9556243B2 (en) | 2013-03-15 | 2017-01-31 | Biomet Biologies, LLC | Methods for making cytokine compositions from tissues using non-centrifugal methods |
US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
US9701728B2 (en) | 2008-02-27 | 2017-07-11 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
US10576130B2 (en) | 2013-03-15 | 2020-03-03 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
US20210039113A1 (en) * | 2018-01-30 | 2021-02-11 | Jun Seok Lee | Piston for centrifugation |
EP4016085A1 (en) | 2020-12-21 | 2022-06-22 | Tecan Trading AG | Iterative liquid aspiration |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3512940A (en) * | 1968-12-30 | 1970-05-19 | Justin J Shapiro | Test tube filter device |
US3786985A (en) * | 1973-01-05 | 1974-01-22 | Hoffmann La Roche | Blood collection container |
-
1974
- 1974-02-27 US US446383A patent/US3897343A/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3508653A (en) * | 1967-11-17 | 1970-04-28 | Charles M Coleman | Method and apparatus for fluid handling and separation |
US3512940A (en) * | 1968-12-30 | 1970-05-19 | Justin J Shapiro | Test tube filter device |
US3786985A (en) * | 1973-01-05 | 1974-01-22 | Hoffmann La Roche | Blood collection container |
Cited By (188)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4152270A (en) * | 1976-05-06 | 1979-05-01 | Sherwood Medical Industries Inc. | Phase separation device |
US4169060A (en) * | 1977-10-25 | 1979-09-25 | Eastman Kodak Company | Blood-collecting and serum-dispensing device |
US4811866A (en) * | 1987-01-02 | 1989-03-14 | Helena Laboratories Corporation | Method and apparatus for dispensing liquids |
EP0311011A3 (en) * | 1987-10-08 | 1990-05-23 | Becton, Dickinson & Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4818386A (en) * | 1987-10-08 | 1989-04-04 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
EP0311011A2 (en) * | 1987-10-08 | 1989-04-12 | Becton, Dickinson and Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4877520A (en) * | 1987-10-08 | 1989-10-31 | Becton, Dickinson And Company | Device for separating the components of a liquid sample having higher and lower specific gravities |
US4892714A (en) * | 1988-10-13 | 1990-01-09 | Microscale Organic Laboratory Corporation | Recrystallization apparatus |
US5266199A (en) * | 1990-11-20 | 1993-11-30 | Nigata Chemicals And Plastics Co., Ltd. | Serum separating apparatus |
US5393674A (en) * | 1990-12-31 | 1995-02-28 | Levine Robert A | Constitutent layer harvesting from a centrifuged sample in a tube |
US5251474A (en) * | 1992-01-16 | 1993-10-12 | Wardlaw Stephen C | Centrifuged material layer measurement in an evacuated tube |
US5248480A (en) * | 1992-05-28 | 1993-09-28 | Diasys Corporation | Apparatus for drawing fluid sample and components thereof |
US5393494A (en) * | 1992-05-28 | 1995-02-28 | Diasys Corporation | Apparatus for drawing fluid sample, components thereof, and slide assembly for use therewith |
US5632895A (en) * | 1993-08-13 | 1997-05-27 | Nigata Engineering Co., Ltd. | Serum separating device and apparatus for serum separation |
WO1996018897A1 (en) * | 1994-12-13 | 1996-06-20 | Coleman Charles M | Separator float for blood collection tubes |
EP0753741A4 (en) * | 1995-01-30 | 1998-04-29 | Niigata Engineering Co Ltd | Component separation member and component separator equipped with said member |
EP0753741A1 (en) * | 1995-01-30 | 1997-01-15 | Niigata Engineering Co., Ltd. | Component separation member and component separator equipped with said member |
US5736033A (en) * | 1995-12-13 | 1998-04-07 | Coleman; Charles M. | Separator float for blood collection tubes with water swellable material |
WO1998005426A2 (en) * | 1996-08-02 | 1998-02-12 | C.A. Greiner & Söhne Gesellschaft Mbh | Sealing device, separating device and collecting receptacle for a collector device |
WO1998005426A3 (en) * | 1996-08-02 | 1998-05-07 | Greiner & Soehne C A | Sealing device, separating device and collecting receptacle for a collector device |
US6277331B1 (en) | 1996-08-02 | 2001-08-21 | C. A. Greiner & Söhne Gesellschaft mbH | Holding device for body fluids and tissues |
WO1998051411A3 (en) * | 1997-05-12 | 1999-05-27 | Greiner & Soehne C A | Separating in a centrifugable container and separating method |
WO1998051411A2 (en) * | 1997-05-12 | 1998-11-19 | C.A. Greiner & Söhne Gesellschaft Mbh | Separating in a centrifugable container and separating method |
AT409725B (en) * | 1997-05-12 | 2002-10-25 | Greiner & Soehne C A | SEPARATOR |
EP1005909A3 (en) * | 1998-12-05 | 2002-06-12 | Becton Dickinson and Company | Centrifuge tube with round separation element, liner and cap |
EP1005910A3 (en) * | 1998-12-05 | 2002-06-12 | Becton Dickinson and Company | Centrifuge tube with cylindrically symmetric separation element, liner and cap |
EP1005909A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Centrifuge tube with round separation element, liner and cap |
EP1005910A2 (en) * | 1998-12-05 | 2000-06-07 | Becton Dickinson and Company | Centrifuge tube with cylindrically symmetric separation element, liner and cap |
US6479298B1 (en) * | 1998-12-05 | 2002-11-12 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US6497325B1 (en) * | 1998-12-05 | 2002-12-24 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6516953B1 (en) | 1998-12-05 | 2003-02-11 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US20110187021A1 (en) * | 1999-12-03 | 2011-08-04 | Becton, Dickinson And Company | Device For Separating Components of a Fluid Sample |
US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US8524171B2 (en) | 1999-12-03 | 2013-09-03 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US9095849B2 (en) | 1999-12-03 | 2015-08-04 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US9682373B2 (en) | 1999-12-03 | 2017-06-20 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US20040166029A1 (en) * | 1999-12-03 | 2004-08-26 | Losada Robert J. | Device for separating components of a fluid sample |
US6793892B1 (en) * | 1999-12-06 | 2004-09-21 | Volker Niermann | Device and method for separating components of a fluid sample |
EP1106252A2 (en) * | 1999-12-06 | 2001-06-13 | Becton, Dickinson and Company | Device and method for collecting, preparation and stabilizing a sample |
EP1106251A2 (en) * | 1999-12-06 | 2001-06-13 | Becton, Dickinson and Company | Device and method for separating components of a fluid sample |
US6803022B2 (en) * | 1999-12-06 | 2004-10-12 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US20020094305A1 (en) * | 1999-12-06 | 2002-07-18 | Dicesare Paul C. | Device and method for separating components of a fluid sample |
EP1106251A3 (en) * | 1999-12-06 | 2002-09-18 | Becton, Dickinson and Company | Device and method for separating components of a fluid sample |
EP1106252A3 (en) * | 1999-12-06 | 2003-10-29 | Becton, Dickinson and Company | Device and method for collecting, preparation and stabilizing a sample |
US9656274B2 (en) | 2000-04-28 | 2017-05-23 | Harvest Technologies Corporation | Blood components separator disk |
US9393575B2 (en) | 2000-04-28 | 2016-07-19 | Harvest Technologies Corporation | Blood components separator disk |
US9393576B2 (en) | 2000-04-28 | 2016-07-19 | Harvest Technologies Corporation | Blood components separator disk |
US20040217046A1 (en) * | 2001-03-30 | 2004-11-04 | Franz Konrad | Holding device, particularly for body fluids, comprising a separating device, and a separating device therefor |
US7188734B2 (en) | 2001-03-30 | 2007-03-13 | Greiner Bio-One Gmbh | Holding device, particularly for bodily fluids, comprising a separating device, and a separating device therefor |
US7223346B2 (en) | 2002-05-03 | 2007-05-29 | Hanuman Llc | Methods and apparatus for isolating platelets from blood |
US8950586B2 (en) | 2002-05-03 | 2015-02-10 | Hanuman Llc | Methods and apparatus for isolating platelets from blood |
US20070034579A1 (en) * | 2002-05-03 | 2007-02-15 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US7837884B2 (en) | 2002-05-03 | 2010-11-23 | Hanuman, Llc | Methods and apparatus for isolating platelets from blood |
US7992725B2 (en) | 2002-05-03 | 2011-08-09 | Biomet Biologics, Llc | Buoy suspension fractionation system |
US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US20050196874A1 (en) * | 2002-05-03 | 2005-09-08 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US8187477B2 (en) | 2002-05-03 | 2012-05-29 | Hanuman, Llc | Methods and apparatus for isolating platelets from blood |
US7470371B2 (en) | 2002-05-03 | 2008-12-30 | Hanuman Llc | Methods and apparatus for isolating platelets from blood |
US20050186120A1 (en) * | 2002-05-03 | 2005-08-25 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
US8603346B2 (en) | 2002-05-24 | 2013-12-10 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US8048321B2 (en) | 2002-05-24 | 2011-11-01 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US8808551B2 (en) | 2002-05-24 | 2014-08-19 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US20060278588A1 (en) * | 2002-05-24 | 2006-12-14 | Woodell-May Jennifer E | Apparatus and method for separating and concentrating fluids containing multiple components |
US9114334B2 (en) | 2002-05-24 | 2015-08-25 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US9897589B2 (en) | 2002-05-24 | 2018-02-20 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7832566B2 (en) | 2002-05-24 | 2010-11-16 | Biomet Biologics, Llc | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
US8163184B2 (en) | 2002-05-24 | 2012-04-24 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US8062534B2 (en) | 2002-05-24 | 2011-11-22 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7374678B2 (en) | 2002-05-24 | 2008-05-20 | Biomet Biologics, Inc. | Apparatus and method for separating and concentrating fluids containing multiple components |
US20060273049A1 (en) * | 2002-05-24 | 2006-12-07 | Leach Michael D | Method and apparatus for separating and concentrating a component from a multi-component material including macroparticles |
US10183042B2 (en) | 2002-05-24 | 2019-01-22 | Biomet Manufacturing, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US10393728B2 (en) | 2002-05-24 | 2019-08-27 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7780860B2 (en) | 2002-05-24 | 2010-08-24 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7914689B2 (en) | 2002-05-24 | 2011-03-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7845499B2 (en) | 2002-05-24 | 2010-12-07 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7179391B2 (en) | 2002-05-24 | 2007-02-20 | Biomet Manufacturing Corp. | Apparatus and method for separating and concentrating fluids containing multiple components |
US7074577B2 (en) | 2002-10-03 | 2006-07-11 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US20080128340A1 (en) * | 2002-10-03 | 2008-06-05 | Thomas Haubert | Buffy coat tube and float system and method |
US7915029B2 (en) | 2002-10-03 | 2011-03-29 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US7329534B2 (en) | 2002-10-03 | 2008-02-12 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US20110171680A1 (en) * | 2002-10-03 | 2011-07-14 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US8012742B2 (en) | 2002-10-03 | 2011-09-06 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US20060154308A1 (en) * | 2002-10-03 | 2006-07-13 | Battelle Memorial Institute | Buffy coat tube and float system and method |
US20040182795A1 (en) * | 2003-03-21 | 2004-09-23 | Randel Dorian | Apparatus and method for concentration of plasma from whole blood |
US6905612B2 (en) | 2003-03-21 | 2005-06-14 | Hanuman Llc | Plasma concentrate apparatus and method |
US20040182788A1 (en) * | 2003-03-21 | 2004-09-23 | Randel Dorian | Plasma concentrate apparatus and method |
US8448800B2 (en) | 2004-01-23 | 2013-05-28 | Greiner Bio-One Gmbh | Method for the assembly of a cap with a receptacle |
US20080223815A1 (en) * | 2004-01-23 | 2008-09-18 | Franz Konrad | Method for the Assembly of a Cap with a Receptacle |
EP2277625A1 (en) | 2004-01-23 | 2011-01-26 | Greiner Bio-One GmbH | Retaining device for blood, body fluids, tissue samples or tissue cultures |
US7901584B2 (en) | 2005-02-07 | 2011-03-08 | Hanuman, Llc | Plasma concentration |
US7987995B2 (en) | 2005-02-07 | 2011-08-02 | Hanuman, Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
US20080011684A1 (en) * | 2005-02-07 | 2008-01-17 | Dorian Randel E | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US8133389B2 (en) | 2005-02-07 | 2012-03-13 | Hanuman, Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
US7708152B2 (en) | 2005-02-07 | 2010-05-04 | Hanuman Llc | Method and apparatus for preparing platelet rich plasma and concentrates thereof |
US7866485B2 (en) | 2005-02-07 | 2011-01-11 | Hanuman, Llc | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US7824559B2 (en) | 2005-02-07 | 2010-11-02 | Hanumann, LLC | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US8096422B2 (en) | 2005-02-07 | 2012-01-17 | Hanuman Llc | Apparatus and method for preparing platelet rich plasma and concentrates thereof |
US8105495B2 (en) | 2005-02-07 | 2012-01-31 | Hanuman, Llc | Method for preparing platelet rich plasma and concentrates thereof |
US20090236297A1 (en) * | 2005-02-07 | 2009-09-24 | Hanuman, Llc | Plasma Concentrator Device |
US9011687B2 (en) | 2005-04-27 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for producing autologous clotting components |
US7694828B2 (en) | 2005-04-27 | 2010-04-13 | Biomet Manufacturing Corp. | Method and apparatus for producing autologous clotting components |
US8551344B2 (en) | 2005-04-27 | 2013-10-08 | Biomet Manufacturing, Llc | Method and apparatus for producing autologous clotting components |
US20070003449A1 (en) * | 2005-06-10 | 2007-01-04 | Mehdi Hatamian | Valve for facilitating and maintaining fluid separation |
US8236258B2 (en) | 2005-08-23 | 2012-08-07 | Biomet Biologics, Llc | Method and apparatus for collecting biological materials |
US20070208321A1 (en) * | 2005-08-23 | 2007-09-06 | Biomet Manufacturing Corp. | Method And Apparatus For Collecting Biological Materials |
US20070075016A1 (en) * | 2005-08-23 | 2007-04-05 | Biomet Manufacturing Corp. | Method and apparatus for collecting biological materials |
US8048297B2 (en) | 2005-08-23 | 2011-11-01 | Biomet Biologics, Llc | Method and apparatus for collecting biological materials |
US8048320B2 (en) | 2005-08-23 | 2011-11-01 | Biomet Manufacturing Corp. | Method and apparatus for collecting biological materials |
US20100255977A1 (en) * | 2005-08-23 | 2010-10-07 | Biomet Manufacturing Corp. | Method and Apparatus for Collecting Biological Materials |
US7771590B2 (en) | 2005-08-23 | 2010-08-10 | Biomet Manufacturing Corp. | Method and apparatus for collecting biological materials |
US8512575B2 (en) | 2005-08-23 | 2013-08-20 | Biomet Biologics, Llc | Method and apparatus for collecting biological materials |
US20070102344A1 (en) * | 2005-10-04 | 2007-05-10 | Franz Konrad | Separating device, holding device and method for separation |
US8567609B2 (en) | 2006-05-25 | 2013-10-29 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US7806276B2 (en) | 2007-04-12 | 2010-10-05 | Hanuman, Llc | Buoy suspension fractionation system |
US9138664B2 (en) | 2007-04-12 | 2015-09-22 | Biomet Biologics, Llc | Buoy fractionation system |
US9649579B2 (en) | 2007-04-12 | 2017-05-16 | Hanuman Llc | Buoy suspension fractionation system |
US8119013B2 (en) | 2007-04-12 | 2012-02-21 | Hanuman, Llc | Method of separating a selected component from a multiple component material |
US8596470B2 (en) | 2007-04-12 | 2013-12-03 | Hanuman, Llc | Buoy fractionation system |
US8328024B2 (en) | 2007-04-12 | 2012-12-11 | Hanuman, Llc | Buoy suspension fractionation system |
US7655124B2 (en) | 2007-10-05 | 2010-02-02 | Mady Attila | Apparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae |
US20090090671A1 (en) * | 2007-10-05 | 2009-04-09 | Mady Attila | Apparatus to assist platelet manipulation to prevent and treat endovascular disease and its sequelae |
US9701728B2 (en) | 2008-02-27 | 2017-07-11 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
US11725031B2 (en) | 2008-02-27 | 2023-08-15 | Biomet Manufacturing, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
US10400017B2 (en) | 2008-02-27 | 2019-09-03 | Biomet Biologics, Llc | Methods and compositions for delivering interleukin-1 receptor antagonist |
US20130196425A1 (en) * | 2008-02-29 | 2013-08-01 | Biomet Biologics, Llc | System and Process for Separating a Material |
US8337711B2 (en) | 2008-02-29 | 2012-12-25 | Biomet Biologics, Llc | System and process for separating a material |
US8801586B2 (en) * | 2008-02-29 | 2014-08-12 | Biomet Biologics, Llc | System and process for separating a material |
US9719063B2 (en) | 2008-02-29 | 2017-08-01 | Biomet Biologics, Llc | System and process for separating a material |
US8012077B2 (en) | 2008-05-23 | 2011-09-06 | Biomet Biologics, Llc | Blood separating device |
US9933344B2 (en) | 2008-07-21 | 2018-04-03 | Becton, Dickinson And Company | Density phase separation device |
US9339741B2 (en) | 2008-07-21 | 2016-05-17 | Becton, Dickinson And Company | Density phase separation device |
US10350591B2 (en) | 2008-07-21 | 2019-07-16 | Becton, Dickinson And Company | Density phase separation device |
US20100160135A1 (en) * | 2008-07-21 | 2010-06-24 | Becton, Dickinson And Company | Density Phase Separation Device |
US9700886B2 (en) | 2008-07-21 | 2017-07-11 | Becton, Dickinson And Company | Density phase separation device |
US9714890B2 (en) | 2008-07-21 | 2017-07-25 | Becton, Dickinson And Company | Density phase separation device |
US8747781B2 (en) | 2008-07-21 | 2014-06-10 | Becton, Dickinson And Company | Density phase separation device |
US8394342B2 (en) | 2008-07-21 | 2013-03-12 | Becton, Dickinson And Company | Density phase separation device |
US9452427B2 (en) | 2008-07-21 | 2016-09-27 | Becton, Dickinson And Company | Density phase separation device |
US9333445B2 (en) | 2008-07-21 | 2016-05-10 | Becton, Dickinson And Company | Density phase separation device |
US8506823B2 (en) * | 2008-12-04 | 2013-08-13 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
US9375661B2 (en) | 2008-12-04 | 2016-06-28 | Cesca Therapeutics, Inc. | Apparatus and method for separating and isolating components of a biological fluid |
US20120193274A1 (en) * | 2008-12-04 | 2012-08-02 | Chapman John R | Apparatus and method for separating and isolating components of a biological fluid |
US20100140182A1 (en) * | 2008-12-04 | 2010-06-10 | Chapman John R | Apparatus and method for separating and isolating components of a biological fluid |
US8511480B2 (en) * | 2008-12-04 | 2013-08-20 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
US8511479B2 (en) * | 2008-12-04 | 2013-08-20 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
US20120122649A1 (en) * | 2008-12-04 | 2012-05-17 | Chapman John R | Apparatus and method for separating and isolating components of a biological fluid |
US8177072B2 (en) | 2008-12-04 | 2012-05-15 | Thermogenesis Corp. | Apparatus and method for separating and isolating components of a biological fluid |
US8783470B2 (en) | 2009-03-06 | 2014-07-22 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
US8187475B2 (en) | 2009-03-06 | 2012-05-29 | Biomet Biologics, Llc | Method and apparatus for producing autologous thrombin |
US8992862B2 (en) | 2009-04-03 | 2015-03-31 | Biomet Biologics, Llc | All-in-one means of separating blood components |
US8313954B2 (en) | 2009-04-03 | 2012-11-20 | Biomet Biologics, Llc | All-in-one means of separating blood components |
US8998000B2 (en) | 2009-05-15 | 2015-04-07 | Becton, Dickinson And Company | Density phase separation device |
US10413898B2 (en) | 2009-05-15 | 2019-09-17 | Becton, Dickinson And Company | Density phase separation device |
US10343157B2 (en) | 2009-05-15 | 2019-07-09 | Becton, Dickinson And Company | Density phase separation device |
US11786895B2 (en) | 2009-05-15 | 2023-10-17 | Becton, Dickinson And Company | Density phase separation device |
US20100288694A1 (en) * | 2009-05-15 | 2010-11-18 | Becton, Dickinson And Company | Density Phase Separation Device |
US11351535B2 (en) | 2009-05-15 | 2022-06-07 | Becton, Dickinson And Company | Density phase separation device |
US9079123B2 (en) | 2009-05-15 | 2015-07-14 | Becton, Dickinson And Company | Density phase separation device |
US9731290B2 (en) | 2009-05-15 | 2017-08-15 | Becton, Dickinson And Company | Density phase separation device |
US10807088B2 (en) | 2009-05-15 | 2020-10-20 | Becton, Dickinson And Company | Density phase separation device |
US9802189B2 (en) | 2009-05-15 | 2017-10-31 | Becton, Dickinson And Company | Density phase separation device |
US10456782B2 (en) | 2009-05-15 | 2019-10-29 | Becton, Dickinson And Company | Density phase separation device |
US9364828B2 (en) | 2009-05-15 | 2016-06-14 | Becton, Dickinson And Company | Density phase separation device |
US9919307B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
US9919309B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
US9919308B2 (en) | 2009-05-15 | 2018-03-20 | Becton, Dickinson And Company | Density phase separation device |
US8794452B2 (en) | 2009-05-15 | 2014-08-05 | Becton, Dickinson And Company | Density phase separation device |
US10376879B2 (en) | 2009-05-15 | 2019-08-13 | Becton, Dickinson And Company | Density phase separation device |
US9011800B2 (en) | 2009-07-16 | 2015-04-21 | Biomet Biologics, Llc | Method and apparatus for separating biological materials |
US9533090B2 (en) | 2010-04-12 | 2017-01-03 | Biomet Biologics, Llc | Method and apparatus for separating a material |
US8591391B2 (en) | 2010-04-12 | 2013-11-26 | Biomet Biologics, Llc | Method and apparatus for separating a material |
US9239276B2 (en) | 2011-04-19 | 2016-01-19 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US9642956B2 (en) | 2012-08-27 | 2017-05-09 | Biomet Biologics, Llc | Apparatus and method for separating and concentrating fluids containing multiple components |
US10208095B2 (en) | 2013-03-15 | 2019-02-19 | Biomet Manufacturing, Llc | Methods for making cytokine compositions from tissues using non-centrifugal methods |
US10143725B2 (en) | 2013-03-15 | 2018-12-04 | Biomet Biologics, Llc | Treatment of pain using protein solutions |
US9950035B2 (en) | 2013-03-15 | 2018-04-24 | Biomet Biologics, Llc | Methods and non-immunogenic compositions for treating inflammatory disorders |
US10441634B2 (en) | 2013-03-15 | 2019-10-15 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
US9895418B2 (en) | 2013-03-15 | 2018-02-20 | Biomet Biologics, Llc | Treatment of peripheral vascular disease using protein solutions |
US10576130B2 (en) | 2013-03-15 | 2020-03-03 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
US11957733B2 (en) | 2013-03-15 | 2024-04-16 | Biomet Manufacturing, Llc | Treatment of collagen defects using protein solutions |
US9556243B2 (en) | 2013-03-15 | 2017-01-31 | Biomet Biologies, LLC | Methods for making cytokine compositions from tissues using non-centrifugal methods |
US9550028B2 (en) | 2014-05-06 | 2017-01-24 | Biomet Biologics, LLC. | Single step desiccating bead-in-syringe concentrating device |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
US9713810B2 (en) | 2015-03-30 | 2017-07-25 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US9757721B2 (en) | 2015-05-11 | 2017-09-12 | Biomet Biologics, Llc | Cell washing plunger using centrifugal force |
US11623228B2 (en) * | 2018-01-30 | 2023-04-11 | Jun Seok Lee | Piston for centrifugation |
US20210039113A1 (en) * | 2018-01-30 | 2021-02-11 | Jun Seok Lee | Piston for centrifugation |
EP4016085A1 (en) | 2020-12-21 | 2022-06-22 | Tecan Trading AG | Iterative liquid aspiration |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3897343A (en) | Plasma separator-hydrostatic pressure type | |
US3887466A (en) | Serum/plasma separator cannula fluid by-pass type centrifugal valve cannula seal | |
US3931010A (en) | Serum/plasma separators with centrifugal valves | |
US3894950A (en) | Serum separator improvement with stretchable filter diaphragm | |
US3957654A (en) | Plasma separator with barrier to eject sealant | |
US3887464A (en) | Serum/plasma separator with centrifugal valve seal | |
US3941699A (en) | Plasma separator with centrifugal valve | |
US3931018A (en) | Assembly for collection, separation and filtration of blood | |
US3945928A (en) | Serum/plasma separators with centrifugal valves | |
US3779383A (en) | Sealed assembly for separation of blood components and method | |
US3849072A (en) | Plasma separator | |
US3894952A (en) | Serum/plasma separator assembly having interface-seeking piston | |
US4202769A (en) | Method for separating serum or plasma from the formed elements of blood | |
US4057499A (en) | Apparatus and method for separation of blood | |
US3920557A (en) | Serum/plasma separator--beads-plus-adhesive type | |
US4055501A (en) | Fluid collection device with phase partitioning means | |
US3935113A (en) | Serum/plasma separator with centrifugal valve | |
US3909419A (en) | Plasma separator with squeezed sealant | |
US3891553A (en) | Serum and plasma separator {13 {0 constrictionless type | |
US4152270A (en) | Phase separation device | |
CA1319659C (en) | Device for separating the components of a liquid sample having higher and lower specific gravities | |
EP0235244B1 (en) | Method and device for separating serum/plasma from blood | |
US3814248A (en) | Method and apparatus for fluid collection and/or partitioning | |
US3894951A (en) | Serum/plasma separator; interface seeking piston; resilient apertures in lower diaphragm type | |
US3919085A (en) | Plasma separator assembly |