US3915805A - Quantitative detection of endotoxin in biological fluids - Google Patents
Quantitative detection of endotoxin in biological fluids Download PDFInfo
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- US3915805A US3915805A US364461A US36446173A US3915805A US 3915805 A US3915805 A US 3915805A US 364461 A US364461 A US 364461A US 36446173 A US36446173 A US 36446173A US 3915805 A US3915805 A US 3915805A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
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- ABSTRACT An in vitro method as well as an article is provided for quantitative detection of endotoxin in biologicals, biological fluids, such as blood, and any protein containing material by an indicator comprising amebocyte lysate.
- This method or technique consists in first centrifuging a blood sample to obtain plasma, and then admixing chloroform with the plasma to precipitate certain protein fractions of the plasma. The mixture of chloroform and plasma is then centrifuged to sediment the precipitated protein and results in the formation of an aqueous layer, an intermediate layer, and a chloroform layer.
- the intermediate layer is then removed, and a clottable substrate, namely amebocyte lysate is then admixed with the removed intermediate layer.
- a clottable substrate namely amebocyte lysate
- the rate of reaction which is proportional to the concentration of endotoxin in the sample is measured as manifested by the increase in turbidity thereof.
- the clottable substrate namely amebocyte lysate, is obtained from amebocytes of Limulus and is provided as an article of manufacture for use in the detection of the endotoxin in the biological fluid sample.
- This invention relates generally to hematology and infectious diseases, and more particularly it pertains to an indicator and method for detecting bacterial endotoxin in protein containing materials, such as blood.
- biologicals are defined as medicinal perparations made from living organisms and their products, including serums, vaccines, antigens, and antitoxins, all of which contain protein
- biological fluids are defined as various substances obtained from living organisms, such as blood, cerebrospinal fluid, exudates, and transudates. It is to be noted that while the present invention is illustrated in connection with determining endotoxin in blood, it is not limited thereto as any biologicals, biological fluids, or protein containing substance can be used in this process and the article pertaining thereto is equally applicable.
- Bacterial endotoxin is defined as some part of the bacterial cell wall of organisms classified as gramnegative, that is, they do not take gram stain. Generally, such organisms contain endotoxin and many of their biological effects and the clinical illnesses they cause are believed clue to the endotoxin which is an intrinsic part of their structures.
- endotoxin while broadly described as a lipopolysaccharide, has not been clearly defined. Which part of the endotoxin molecule is responsible for its biological effects in certain clinical illnesses is still not clear.
- test would be by pharmaceutical companies or other facilities that require pyrogen testing; that is, detection of the presence of endotoxin in biologicals, biological fluids, and other protein containing materials for human use.
- Another object of this invention is to provide a method of preparing a specimen of biological fluid (i.e., blood) for endotoxin testing in a manner which yields a high sensitivity.
- biological fluid i.e., blood
- Clottable protein in the amebocytes of the horseshoe crab is a sensitive indicator of bacterial endotoxin.
- Table I shows the effect of endotoxin concentration upon the rate of increase in light scattering, where the reaction mixture consisted of 0.9 ml of lysate (clottable substrate) and 0.1 ml of endotoxin. The final endotoxin concentration (#g/ml) is shown. It is to be noted that the A light scattering was the maximum percent increase in light scattering per minute.
- the plasma and serum were separated from blood to which endotoxin had been added. and then tested for endotoxin activity after their removal from the blood cells or fibrin clot.
- NaCl indicates the controls in which identical concentrations of endotoxin were present in saline. in every instance in which endotoxin was detected in plasma and saline. the rate of gelation was faster in the NaCl control.
- the blood sample in which endotoxin is suspected to be present is centrifuged so that the plasma is separated from the blood cells.
- the plasma which is obtained from the heparinized blood is then mixed with chloroform, one part chloroform to four parts plasma.
- This intermediate (middle) layer contains a concentration of detectable endotoxin almost equal to that present in the original sample of whole blood. However, endotoxin can also be detected in the top layer, and there are instances when this layer may be used.
- the cloudy middle layer is removed by pipette or needle and syringe and mixed with an equal volume of amebocyte lysate.
- the incubation mixture is incubated at 37C. in a water bath and observed visually. Other temperatures can be selected for the incubation.
- the reaction occurs at a rate proportional to the concentration of endotoxin in the blood or other solution tested. It is manifested by an increase in cloudiness of the mixture, which can be recognized with the eye or measured more accurately with any type of light scattering apparatus. Thus, increased turbidity of the mixture indicates the presence of endotoxin. It is also to be understood that increased viscosity of the mixture indicates the presence of endotoxin.
- the assay mixture incubate the assay mixture in a 37C. water bath and visually observe the reaction, first at short intervals, and then, at longer intervals.
- the increase in light scattering in the incubation mixture may be monitored on a turbidity meter (nephelometrically).
- the clottable substrate referred to herein essentially comprises plasma-free Limulus amebocytes which are separated from plasma in a sample of Limulus blood, the Limulus used being the horseshoe crab.
- the blood is drawn directly into sterile, pyrogen free, siliconized syringes or flasks preheated to 40C and containing tris-buffered N-ethyl maleimide (NEM) at pH 7.28 and 40C.
- NEM N-ethyl maleimide
- the ratio of whole blood to NEM is 1:1 producing a final concentration of NEM of X IOM.
- the NEM is used to prevent aggregation.
- amebocytes are then twice washed and resuspended in artificial buffered sea water. These Washed cells are then lysed by freezing and thawing four times in dry ice in acetone, or by exposure to distilled water. The cell lysate is then cleared by centrifugation at 2000 rpm for five minutes, yielding a clear colorless liquid composed of cellular lysate in artificial sea water or distilled water, which then may be used to detect endotoxin. This form of the material is stable and will keep for many months at 4C. without losing potency.
- the secondmentioned step comprises centrifuging the sample from which certain proteins have been precipitated and removing said precipitated proteins from the sample.
- An in vitro method for quantitative detection of endotoxin in a protein containing material comprising, admixing chloroform with said protein containing material to precipitate certain proteins from said protein containing material, centrifuging said precipitated proteins to obtain a sample contaminated with endotoxin, admixing Limulus amebocyte lysate with said sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
- An in vitro method for quantitative detection of endotoxin in biological fluids comprising, admixing chloroform with a biological fluid to precipitate certain proteins of said biological fluid, centrifuging said mixture of chloroform and biological fluid to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
- An in vitro method for quantitive detection of endotoxin in blood comprising, admixing chloroform with a blood plasma to precipitate certain proteins of said plasma, centrifuging said mixture of chloroform and plasma to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof.
Abstract
An in vitro method as well as an article is provided for quantitative detection of endotoxin in biologicals, biological fluids, such as blood, and any protein containing material by an indicator comprising amebocyte lysate. This method or technique consists in first centrifuging a blood sample to obtain plasma, and then admixing chloroform with the plasma to precipitate certain protein fractions of the plasma. The mixture of chloroform and plasma is then centrifuged to sediment the precipitated protein and results in the formation of an aqueous layer, an intermediate layer, and a chloroform layer. The intermediate layer is then removed, and a clottable substrate, namely amebocyte lysate is then admixed with the removed intermediate layer. The rate of reaction which is proportional to the concentration of endotoxin in the sample is measured as manifested by the increase in turbidity thereof. The clottable substrate, namely amebocyte lysate, is obtained from amebocytes of Limulus and is provided as an article of manufacture for use in the detection of the endotoxin in the biological fluid sample.
Description
i United States Patent [191 Levin [4 1 Oct. 28, 1975 QUANTITATIVE DETECTION OF ENDOTOXIN IN BIOLOGICAL FLUIDS [75] Inventor: Jack Levin, Baltimore, Md.
[73] Assignee: The Johns Hopkins University,
Baltimore, Md.
[22] Filed: May 29, 1973 [21] Appl. No.: 364,461
abandoned.
[52] US. Cl. 195/103.5 R; 23/230 B; 424/2; 424/95; 424/101 [51] Int. Cl. G01N 31/00; C12K 1/00 [58] Field of Search l95/l03.5; 23/230 B; 424/95, 101
[56] References Cited UNITED STATES PATENTS 3,408,259 10/1968 Kruger et al. 195/103.5 R 3,579,495 5/1971 Huber 424/101 OTHER PUBLICATIONS Levin, J., A Sensitive System to Detect Endotoxin in Vitro. Pathology No. 791, Fed. Proc. 27 (p. 355) 1968. Levin, et al., Clottable Protein in Limulus, Chemical 'Abstracts, Vol. 69, M68 (p. 853, 9114x).
Levin, et al., The Role of Endotoxin in the Extracellular Coagulation of Limulus Blood, Bull. Hopkins Hosp., Vol. 115, 1964 (pp. 265-274).
Levin, et al., A Description of Cellular Coagulation in the Limulus, Bull. Hopkins Hosp., Vol. 1 15, 1964 (pp. 337-345).
Corona, et al., The Limulus Coagulation Test for Endotoxin, A Comparison With Other Assay Methods, Proc. Soc. Exptl. Biol. Med. Vol. 132, 1969 (PP. 599-601 l-lo, et al., Protective Effect of Components of Normal Blood Against the Lethal Action of Endotoxin, J. Lab. & Clin. Med. Vol. 5121, 1958 (pp.297-311).
Primary Examiner-David M. Naff Attorney, Agent, or Firm-Walter G. Finch [57] ABSTRACT An in vitro method as well as an article is provided for quantitative detection of endotoxin in biologicals, biological fluids, such as blood, and any protein containing material by an indicator comprising amebocyte lysate. This method or technique consists in first centrifuging a blood sample to obtain plasma, and then admixing chloroform with the plasma to precipitate certain protein fractions of the plasma. The mixture of chloroform and plasma is then centrifuged to sediment the precipitated protein and results in the formation of an aqueous layer, an intermediate layer, and a chloroform layer. The intermediate layer is then removed, and a clottable substrate, namely amebocyte lysate is then admixed with the removed intermediate layer. The rate of reaction which is proportional to the concentration of endotoxin in the sample is measured as manifested by the increase in turbidity thereof. The clottable substrate, namely amebocyte lysate, is obtained from amebocytes of Limulus and is provided as an article of manufacture for use in the detection of the endotoxin in the biological fluid sample.
7 Claims, No Drawings QUANTITATIVE DETECTION OF ENDOTOXIN IN BIOLOGICAL FLUIDS This is a continuation of application Ser. No. 40,348, filed May 25, 1970, now abandoned.
This invention relates generally to hematology and infectious diseases, and more particularly it pertains to an indicator and method for detecting bacterial endotoxin in protein containing materials, such as blood.
In connection with this invention, biologicals are defined as medicinal perparations made from living organisms and their products, including serums, vaccines, antigens, and antitoxins, all of which contain protein, while biological fluids are defined as various substances obtained from living organisms, such as blood, cerebrospinal fluid, exudates, and transudates. It is to be noted that while the present invention is illustrated in connection with determining endotoxin in blood, it is not limited thereto as any biologicals, biological fluids, or protein containing substance can be used in this process and the article pertaining thereto is equally applicable.
Bacterial endotoxin is defined as some part of the bacterial cell wall of organisms classified as gramnegative, that is, they do not take gram stain. Generally, such organisms contain endotoxin and many of their biological effects and the clinical illnesses they cause are believed clue to the endotoxin which is an intrinsic part of their structures.
The chemical nature of endotoxin while broadly described as a lipopolysaccharide, has not been clearly defined. Which part of the endotoxin molecule is responsible for its biological effects in certain clinical illnesses is still not clear.
The significance of a positive test would be that in the case of a patient, the physician would know that he had detected endotoxin in the blood (i.e. endotoxemia). He then would be able to initiate appropriate therapy for the effects of the endotoxin or for an underlying disease which might account for its presence.
Another use of the test would be by pharmaceutical companies or other facilities that require pyrogen testing; that is, detection of the presence of endotoxin in biologicals, biological fluids, and other protein containing materials for human use.
Conventional methods for the detection of endotoxin have in the past involved biological assays using test animals, such as rabbits. The response of the animal was taken as a measure of the presence of endotoxin in the fluid administered.
It is an object of this invention, therefore, to provide a simple and rapid method for the detection of endotoxin, which is clinically practical and which can be done in vitro (i.e., does not require test animals).
Another object of this invention is to provide a method of preparing a specimen of biological fluid (i.e., blood) for endotoxin testing in a manner which yields a high sensitivity.
Clottable protein in the amebocytes of the horseshoe crab (Limulus) is a sensitive indicator of bacterial endotoxin. Table I below, for example, shows the effect of endotoxin concentration upon the rate of increase in light scattering, where the reaction mixture consisted of 0.9 ml of lysate (clottable substrate) and 0.1 ml of endotoxin. The final endotoxin concentration (#g/ml) is shown. It is to be noted that the A light scattering was the maximum percent increase in light scattering per minute.
TABLE I Effect of Endotoxin Concentration Upon the Rate of Increase in Light Scattering Endotoxin Latent Period A Light Scattering Lg/ml) (min) (Maximum/min) TABLE II Effect of Blood and Blood Coagulation on the Detection of Enclotoxin, in vitro Initial Endotoxin Concentration (pg/ml) Plasma Serum NaCl 0.001 Neg. Neg. Pos. 0.02 Pos. Neg. Pos. 0.l Pos. Neg. Pos. 0.2 Pos. Neg. Pos. 0.4 Neg. Neg. Pos. 2.0 Pos. Pos. Pos. 5.0 Pos. Neg. Pos.
It is to be noted that the plasma and serum (each obtained from the same donor) were separated from blood to which endotoxin had been added. and then tested for endotoxin activity after their removal from the blood cells or fibrin clot. NaCl indicates the controls in which identical concentrations of endotoxin were present in saline. in every instance in which endotoxin was detected in plasma and saline. the rate of gelation was faster in the NaCl control.
The decrease in the ability to detect endotoxin after exposure to blood is presumably the result of inactivation of the biologically active (endotoxic) polysaccharides by serum or plasma.
According to the present invention, a technique has been devised for the preparation of blood samples so that endotoxin may be detected by the aforesaid clottable protein. See Table 111 below which gives data relating to the recovery of endotoxin activity from plasma following extraction with-chloroform.
The blood sample in which endotoxin is suspected to be present is centrifuged so that the plasma is separated from the blood cells. The plasma which is obtained from the heparinized blood is then mixed with chloroform, one part chloroform to four parts plasma.
The mixture is then vigorously agitated for one hour and the material is then re-centrifuged so that the aqueous layer is on top and the chloroform layer containing precipitated protein is at the bottom. This results in an interface between the aqueous and chloroform layers which has a cloudy appearance. This intermediate (middle) layer contains a concentration of detectable endotoxin almost equal to that present in the original sample of whole blood. However, endotoxin can also be detected in the top layer, and there are instances when this layer may be used.
The cloudy middle layer is removed by pipette or needle and syringe and mixed with an equal volume of amebocyte lysate. The incubation mixture is incubated at 37C. in a water bath and observed visually. Other temperatures can be selected for the incubation. The reaction occurs at a rate proportional to the concentration of endotoxin in the blood or other solution tested. It is manifested by an increase in cloudiness of the mixture, which can be recognized with the eye or measured more accurately with any type of light scattering apparatus. Thus, increased turbidity of the mixture indicates the presence of endotoxin. It is also to be understood that increased viscosity of the mixture indicates the presence of endotoxin.
During the observation, it is desirable to incubate the assay mixture in a 37C. water bath and visually observe the reaction, first at short intervals, and then, at longer intervals. Optionally, the increase in light scattering in the incubation mixture may be monitored on a turbidity meter (nephelometrically).
TABLE III Recovery of Endotoxin Activity from Plasma Following Extraction with Chloroform* The incubation mixture consisted of 1 part middle layer and 1 part amebocyte lysate. The fractions indicate the number of positive tests/total number of experiments at each endotoxin concentration. Thirty-eight different blood donors were used in these experiments.
"Endotoxin was added to plasma.
'"Endotoxin was initially added to whole heparinized blood before the plasma was prepared.
The clottable substrate referred to herein essentially comprises plasma-free Limulus amebocytes which are separated from plasma in a sample of Limulus blood, the Limulus used being the horseshoe crab. The blood is drawn directly into sterile, pyrogen free, siliconized syringes or flasks preheated to 40C and containing tris-buffered N-ethyl maleimide (NEM) at pH 7.28 and 40C. The ratio of whole blood to NEM is 1:1 producing a final concentration of NEM of X IOM. The NEM is used to prevent aggregation.
After centrifuging the blood samples at 600 rpm for one minute, or allowing the cells to sediment for one hour, the supernatent material is poured off and the sedimented cells resuspended in ml. of buffered NEM, l X 10' at 40C. This procedure is repeated twice.
The amebocytes are then twice washed and resuspended in artificial buffered sea water. These Washed cells are then lysed by freezing and thawing four times in dry ice in acetone, or by exposure to distilled water. The cell lysate is then cleared by centrifugation at 2000 rpm for five minutes, yielding a clear colorless liquid composed of cellular lysate in artificial sea water or distilled water, which then may be used to detect endotoxin. This form of the material is stable and will keep for many months at 4C. without losing potency.
Obviously many modifications and variations of the present invention are possible in light of the above teachings. It is, therefore, to be understood that within the scope of the appended claims the invention may be practiced otherwise than as specifically described.
What is claimed is:
1. An in vitro method for quantitative detection of endotoxin in a sample of protein-containing material,
comprising:
admixing chloroform with the sample to precipitate certain proteins therefrom;
separating the precipitated proteins from the sample;
admixing lysate of plasma-free Limulus amebocytes with said sample from which the precipitated proteins have been separated; and, measuring the rate of reaction between endotoxin in the sample and the lysate, the rate of reaction being proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof. 2. The method of claim 1 wherein the secondmentioned step comprises centrifuging the sample from which certain proteins have been precipitated and removing said precipitated proteins from the sample.
3. The method of claim 1 and further comprising: sedimenting the sample to which chloroform has been added to form an aqueous layer, an intermediate layer, and a chloroform layer in the sample;
removing at least a portion of said intermediate layer;
and,
admixing said removed portion of said intermediate layer with lysate of plasma-free Limulus amebocytes.
4. An in vitro method for quantitative detection of endotoxin in a protein containing material, comprising, admixing chloroform with said protein containing material to precipitate certain proteins from said protein containing material, centrifuging said precipitated proteins to obtain a sample contaminated with endotoxin, admixing Limulus amebocyte lysate with said sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
5. An in vitro method for quantitative detection of endotoxin in biological fluids, comprising, admixing chloroform with a biological fluid to precipitate certain proteins of said biological fluid, centrifuging said mixture of chloroform and biological fluid to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
6. An in vitro method for quantitive detection of endotoxin in blood, comprising, admixing chloroform with a blood plasma to precipitate certain proteins of said plasma, centrifuging said mixture of chloroform and plasma to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof.
ple from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample layer, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof.
Claims (7)
1. AN IN VITRO METHOD FOR QUANTITATIVE DETECTION OF ENDOTOXIN IN A SAMPLE OF PROTEIN-CONTAINING MATERIAL, COMPRISING: ADMIXING CHLOROFORM WITH THE SAMPLE TO PRECIPITATE CERTAIN PROTEINS THEREFROM, SETPARATING THE PRECIPITATED PROTEINS FROM THE SAMPLE, ADMIXING LYSTER OF PLASMA-FREE LIMULUS AMEBOCYTES WITH SAID SAMPLE FROM WHICH THE PRECIPITATED PROTEINS HAVE BEEN SEPARATED, AND, MEASURING THE RATE OF REACTION BETWEEN ENDOTOXIN IN THE SAMPLY AND THE LYSATE, THE RATE OF REACTION BEING PROPORTIONAL TO THE CONCENTRATION OF ENDOTOXIN IN THE SAMPLY AS MANIFESTED BY THE INCREASED TURBIDITY OR VISCOSITY THEREOF.
2. The method of claim 1 wherein the second-mentioned step comprises centrifuging the sample from which certain proteins have been precipitated and removing said precipitated proteins from the sample.
3. The method of claim 1 and further comprising: sedimenting the sample to which chloroform has been added to form an aqueous layer, an intermediate layer, and a chloroform layer in the sample; removing at least a portion of said intermediate layer; and, admixing said removed portion of said intermediate layer with lysate of plasma-free Limulus amebocytes.
4. An in vitro method for quantitative detection of endotoxin in a protein containing material, comprising, admixing chloroform with said protein containing material to precipitate certain proteins from said protein containing material, centrifuging said precipitated proteins to obtain a sample contaminated with endotoxin, admixing Limulus amebocyte lysate with said sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
5. An in vitro method for quantitative detection of endotoxin in biological fluids, comprising, admixing chloroform with a biological fluid to precipitate certain proteins of said biological fluid, centrifuging said mixture of chloroform and biological fluid to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in said sample as manifested by the increased turbidity or viscosity thereof.
6. An in vitro method for quantitative detection of endotoxin in blood, comprising, admixing chloroform with a blood plasma to precipitate certain proteins of said plasma, centrifuging said mixture of chloroform and plasma to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample, and then measUring the rate of reaction which is proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof.
7. An in vitro method for quantitative detection of endotoxin in blood, comprising, centrifuging a sample of blood to obtain plasma, admixing chloroform with said plasma to precipitate certain proteins of said plasma, centrifuging said mixture of chloroform and biologial fluid to sediment the precipitated proteins resulting in the formation of an aqueous layer, an intermediate layer, and a chloroform layer, removing a sample from said intermediate layer, admixing Limulus amebocyte lysate with said removed sample layer, and then measuring the rate of reaction which is proportional to the concentration of endotoxin in the sample as manifested by the increased turbidity or viscosity thereof.
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| US364461A US3915805A (en) | 1970-05-25 | 1973-05-29 | Quantitative detection of endotoxin in biological fluids |
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| US4034870A | 1970-05-25 | 1970-05-25 | |
| US364461A US3915805A (en) | 1970-05-25 | 1973-05-29 | Quantitative detection of endotoxin in biological fluids |
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Cited By (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4038147A (en) * | 1976-06-15 | 1977-07-26 | Becton, Dickinson And Company | Method for detecting endotoxins |
| US4038029A (en) * | 1976-01-20 | 1977-07-26 | Worthington Biochemical Corporation | Limulus lysate turbidity test for pyrogens |
| US4104030A (en) * | 1977-11-04 | 1978-08-01 | Baxter Travenol Laboratories, Inc. | Photometric determination of protein, and of endotoxin with Limulus protein |
| US4107077A (en) * | 1975-07-14 | 1978-08-15 | Associates Of Cape Cod, Inc. | Limulus lysate of improved sensitivity and preparing the same |
| DE2722077A1 (en) * | 1977-05-16 | 1978-11-23 | Worthington Biochem Corp | Turbidity test for endotoxins - useful for quantitative tests with e.g. biological fluids, drugs and parental compsns |
| US4188264A (en) * | 1977-06-14 | 1980-02-12 | Seikagaku Kogyo Co. Ltd. | Process for determining bacterial endotoxin and reagents used therefor |
| DE2927103A1 (en) * | 1978-10-06 | 1980-04-17 | Baxter Travenol Lab | OPTICAL METHOD FOR DETERMINING ENDOTOXIN |
| US4221865A (en) * | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4221866A (en) * | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4279774A (en) * | 1979-11-13 | 1981-07-21 | Dynasciences Corporation | Method of removing lipid inhibitors from limulus amebocyte lysate |
| US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| EP0049161A2 (en) * | 1980-09-30 | 1982-04-07 | Green Cross Corporation | Method for preparing sample for use in endotoxin test and kit for preparing said sample |
| US4370413A (en) * | 1980-02-14 | 1983-01-25 | Technion Research And Development Foundation, Ltd. | Micromethod for the determination of endotoxins |
| US4376819A (en) * | 1979-09-14 | 1983-03-15 | Baxter Travenol Laboratories, Inc. | Biological extracts and method for making same |
| DE3149117A1 (en) * | 1981-12-11 | 1983-06-23 | Sartorius GmbH, 3400 Göttingen | Method and apparatus for preparation for the qualitative and quantitative determination of bacterial endotoxins (pyrogens) in aqueous solutions |
| US4476093A (en) * | 1980-09-30 | 1984-10-09 | The Green Cross Corporation | Kit for preparing sample for use in endotoxin test |
| US4906567A (en) * | 1987-01-21 | 1990-03-06 | E. I. Dupont De Nemours And Company | Non-immunochemical binding of lipopolysaccharides and sandwich assays therefor |
| WO1991006867A1 (en) * | 1989-11-01 | 1991-05-16 | Atlantic Institute Of Biotechnology | Echinoderm agglutinin and method of extraction |
| US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
| US6391295B1 (en) | 1999-10-25 | 2002-05-21 | Associates Of Cape Cod, Inc. | Artificial bait |
| US20030229211A1 (en) * | 1988-06-23 | 2003-12-11 | Wainwright Norman R. | Endotoxin binding and neutralizing protein and uses thereof |
| US20060183181A1 (en) * | 2004-12-02 | 2006-08-17 | Wainwright Norman R | Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants |
| US20060216780A1 (en) * | 2005-01-13 | 2006-09-28 | Wainwright Norman R | Method for classifying a microorganism in a biological sample |
| US20080020422A1 (en) * | 2003-03-17 | 2008-01-24 | Wainwright Norman R | Methods and Compositions for the Detection of Microbial Contaminants |
| US20090286692A1 (en) * | 2008-04-15 | 2009-11-19 | Wainwright Norman R | Cartridge and Method for Sample Analysis |
| EP2306177A1 (en) * | 2008-07-30 | 2011-04-06 | Kowa Company, Ltd. | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| CN104048953A (en) * | 2013-03-11 | 2014-09-17 | 华中科技大学 | Quick detection method and kit for trace-amount endotoxin |
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| US3408259A (en) * | 1966-01-21 | 1968-10-29 | Inst Mitchforschung Oranienbur | Process for detecting staphylococci-enterotoxin in foodstuffs |
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1973
- 1973-05-29 US US364461A patent/US3915805A/en not_active Expired - Lifetime
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Cited By (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4107077A (en) * | 1975-07-14 | 1978-08-15 | Associates Of Cape Cod, Inc. | Limulus lysate of improved sensitivity and preparing the same |
| US4038029A (en) * | 1976-01-20 | 1977-07-26 | Worthington Biochemical Corporation | Limulus lysate turbidity test for pyrogens |
| US4038147A (en) * | 1976-06-15 | 1977-07-26 | Becton, Dickinson And Company | Method for detecting endotoxins |
| DE2722077A1 (en) * | 1977-05-16 | 1978-11-23 | Worthington Biochem Corp | Turbidity test for endotoxins - useful for quantitative tests with e.g. biological fluids, drugs and parental compsns |
| US4188264A (en) * | 1977-06-14 | 1980-02-12 | Seikagaku Kogyo Co. Ltd. | Process for determining bacterial endotoxin and reagents used therefor |
| US4104030A (en) * | 1977-11-04 | 1978-08-01 | Baxter Travenol Laboratories, Inc. | Photometric determination of protein, and of endotoxin with Limulus protein |
| DE2927103A1 (en) * | 1978-10-06 | 1980-04-17 | Baxter Travenol Lab | OPTICAL METHOD FOR DETERMINING ENDOTOXIN |
| US4221865A (en) * | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4221866A (en) * | 1978-10-06 | 1980-09-09 | Baxter Travenol Laboratories, Inc. | Method for determining endotoxin concentration |
| US4376819A (en) * | 1979-09-14 | 1983-03-15 | Baxter Travenol Laboratories, Inc. | Biological extracts and method for making same |
| US4279774A (en) * | 1979-11-13 | 1981-07-21 | Dynasciences Corporation | Method of removing lipid inhibitors from limulus amebocyte lysate |
| US4370413A (en) * | 1980-02-14 | 1983-01-25 | Technion Research And Development Foundation, Ltd. | Micromethod for the determination of endotoxins |
| US4322217A (en) * | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| EP0049161A3 (en) * | 1980-09-30 | 1982-05-26 | The Green Cross Corporation | Method for preparing sample for use in endotoxin test and kit for preparing said sample |
| EP0049161A2 (en) * | 1980-09-30 | 1982-04-07 | Green Cross Corporation | Method for preparing sample for use in endotoxin test and kit for preparing said sample |
| US4476093A (en) * | 1980-09-30 | 1984-10-09 | The Green Cross Corporation | Kit for preparing sample for use in endotoxin test |
| DE3149117A1 (en) * | 1981-12-11 | 1983-06-23 | Sartorius GmbH, 3400 Göttingen | Method and apparatus for preparation for the qualitative and quantitative determination of bacterial endotoxins (pyrogens) in aqueous solutions |
| US4906567A (en) * | 1987-01-21 | 1990-03-06 | E. I. Dupont De Nemours And Company | Non-immunochemical binding of lipopolysaccharides and sandwich assays therefor |
| US20030229211A1 (en) * | 1988-06-23 | 2003-12-11 | Wainwright Norman R. | Endotoxin binding and neutralizing protein and uses thereof |
| WO1991006867A1 (en) * | 1989-11-01 | 1991-05-16 | Atlantic Institute Of Biotechnology | Echinoderm agglutinin and method of extraction |
| US6270982B1 (en) | 1997-10-09 | 2001-08-07 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
| US6391570B1 (en) | 1997-10-09 | 2002-05-21 | Charles River Laboratories | Methods and compositions for the detection of bacterial endotoxins |
| US6391295B1 (en) | 1999-10-25 | 2002-05-21 | Associates Of Cape Cod, Inc. | Artificial bait |
| US7939291B2 (en) | 2003-03-17 | 2011-05-10 | Charles River Laboratories, Inc. | Methods for the detection of microbial contaminants |
| US10119969B2 (en) | 2003-03-17 | 2018-11-06 | Charles River Laboratories, Inc. | Compositions for the detection of microbial contaminants |
| US20080020422A1 (en) * | 2003-03-17 | 2008-01-24 | Wainwright Norman R | Methods and Compositions for the Detection of Microbial Contaminants |
| US7329538B2 (en) | 2003-03-17 | 2008-02-12 | Charles River Laboratories, Inc. | Methods and compositions for the detection of microbial contaminants |
| US20060183181A1 (en) * | 2004-12-02 | 2006-08-17 | Wainwright Norman R | Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants |
| US8440394B2 (en) | 2004-12-02 | 2013-05-14 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| US7968280B2 (en) | 2004-12-02 | 2011-06-28 | Charles River Laboratories, Inc. | Methods for the detection and/or quantification of gram positive bacterial contaminants |
| US7479375B2 (en) | 2005-01-13 | 2009-01-20 | Charles River Laboratories, Inc. | Method for classifying a microorganism in a biological sample |
| US7901899B1 (en) | 2005-01-13 | 2011-03-08 | Charles River Laboratories, Inc. | Method for classifying a microorganism in a biological sample |
| US20060216780A1 (en) * | 2005-01-13 | 2006-09-28 | Wainwright Norman R | Method for classifying a microorganism in a biological sample |
| US20090286692A1 (en) * | 2008-04-15 | 2009-11-19 | Wainwright Norman R | Cartridge and Method for Sample Analysis |
| EP2306177A1 (en) * | 2008-07-30 | 2011-04-06 | Kowa Company, Ltd. | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| US20110124036A1 (en) * | 2008-07-30 | 2011-05-26 | Kowa Company, Ltd. | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| CN102112866A (en) * | 2008-07-30 | 2011-06-29 | 兴和株式会社 | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| EP2306177A4 (en) * | 2008-07-30 | 2012-03-28 | Kowa Co | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| US8697351B2 (en) | 2008-07-30 | 2014-04-15 | Kowa Company, Ltd. | Method for measurement of physiologically active substance derived from organism and measurement apparatus |
| CN104048953A (en) * | 2013-03-11 | 2014-09-17 | 华中科技大学 | Quick detection method and kit for trace-amount endotoxin |
| CN104048953B (en) * | 2013-03-11 | 2016-08-31 | 华中科技大学 | The method for quick of a kind of trace levels of endotoxin and kit |
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