WO1994021788A3 - Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) - Google Patents

Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) Download PDF

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Publication number
WO1994021788A3
WO1994021788A3 PCT/CA1994/000130 CA9400130W WO9421788A3 WO 1994021788 A3 WO1994021788 A3 WO 1994021788A3 CA 9400130 W CA9400130 W CA 9400130W WO 9421788 A3 WO9421788 A3 WO 9421788A3
Authority
WO
WIPO (PCT)
Prior art keywords
maculans
dna
infestation
testing
pcr
Prior art date
Application number
PCT/CA1994/000130
Other languages
French (fr)
Other versions
WO1994021788A2 (en
Inventor
Janet L Taylor
Original Assignee
Ca Nat Research Council
Janet L Taylor
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ca Nat Research Council, Janet L Taylor filed Critical Ca Nat Research Council
Priority to US08/521,053 priority Critical patent/US5916744A/en
Priority to EP94908903A priority patent/EP0689591B1/en
Publication of WO1994021788A2 publication Critical patent/WO1994021788A2/en
Publication of WO1994021788A3 publication Critical patent/WO1994021788A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Abstract

A method of testing for infestation of tissue of rape or other Cruciferae with a virulent strain of Leptosphaeria maculans. The method involves isolating DNA of L. maculans from the tissue; subjecting the isolated DNA to amplification by the polymerase chain reaction (PCR) using sets of primers derived from LMR1 [SEQ ID NO:11] (Genbank accession number M77515), a repetitive element of L. maculans specific to virulent strains of the fungus, to form a product containing amplified L. maculans DNA; preferably separating the amplified L. maculans DNA from the product; and detecting the presence of the amplified separated L. maculans DNA. The invention also relates to a method of deriving DNA of L. maculans from plant tissue for amplification by PCR, to the oligonucleotides forming the primers used in the PCR and to a diagnostic test kit which makes use of the method of testing.
PCT/CA1994/000130 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation) WO1994021788A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US08/521,053 US5916744A (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus Leptosphaeria maculans (blackleg infestation)
EP94908903A EP0689591B1 (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA2,092,115 1993-03-22
CA002092115A CA2092115C (en) 1993-03-22 1993-03-22 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Publications (2)

Publication Number Publication Date
WO1994021788A2 WO1994021788A2 (en) 1994-09-29
WO1994021788A3 true WO1994021788A3 (en) 1994-12-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1994/000130 WO1994021788A2 (en) 1993-03-22 1994-03-14 Testing for infestation of rapeseed and other cruciferae by the fungus leptosphaeria maculans (blackleg infestation)

Country Status (4)

Country Link
US (1) US5916744A (en)
EP (1) EP0689591B1 (en)
CA (1) CA2092115C (en)
WO (1) WO1994021788A2 (en)

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US6406857B1 (en) 1997-06-16 2002-06-18 Exact Sciences Corporation Methods for stool sample preparation
CA2362251C (en) 1999-02-25 2008-06-17 Exact Sciences Corporation Methods for preserving dna integrity
EP1169479B1 (en) 1999-04-09 2006-06-28 EXACT Sciences Corporation Methods for detecting nucleic acids indicative of cancer
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
US6586177B1 (en) * 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
ATE458831T1 (en) * 1999-12-07 2010-03-15 Exact Sciences Corp METHOD FOR DETECTING LUNG NEOPLASMS IN FECAL SAMPLES
US6919174B1 (en) * 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
WO2003068988A1 (en) * 2002-02-11 2003-08-21 Secretary, Department Of Atomic Energy A method of pcr based detection and identification of rice blast fungus magnaporthe grisea
AU2003213107A1 (en) 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
AU2003254298A1 (en) 2002-08-02 2004-02-23 Stratatech Corporation Species specific dna detection
US20040259101A1 (en) * 2003-06-20 2004-12-23 Shuber Anthony P. Methods for disease screening
WO2005113769A1 (en) * 2004-05-14 2005-12-01 Exact Sciences Corporation Method for stabilizing biological samples for nucleic acid analysis
WO2009043182A1 (en) * 2007-10-05 2009-04-09 University Of Manitoba Sequence-characterized amplified region (scar) markers for plant resistance against fungal pathogens
US20110148155A1 (en) * 2009-12-22 2011-06-23 Mattel, Inc. Collapsible Infant Support
CN101805794B (en) * 2010-04-08 2012-05-02 中华人民共和国上海出入境检验检疫局 Real-time fluorescent PCR detection method of L.maculans as well as primer and probe used for detection
CN101805793B (en) * 2010-04-08 2012-04-18 中华人民共和国上海出入境检验检疫局 PCR detection method of L.maculans and primer used for detection
CN103255207A (en) * 2013-01-15 2013-08-21 内蒙古农牧业科学院 Molecular detection method for brassica campestris seeds carrying brassica campestris leptosphaeria maculans pathogenic bacterial
CN103131778A (en) * 2013-02-05 2013-06-05 内蒙古农牧业科学院 Molecular detection method of rapeseed-carried rapeseed blackleg pathogen

Citations (1)

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Publication number Priority date Publication date Assignee Title
DE4127862A1 (en) * 1991-08-21 1993-02-25 Inst Genbiologische Forschung Aggressive and non-aggressive pathotype distinction of phoma lingam - using random primers of 8-11 nucleotide(s) giving differing pattern after gel-electrophoresis, useful in plant protection

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4127862A1 (en) * 1991-08-21 1993-02-25 Inst Genbiologische Forschung Aggressive and non-aggressive pathotype distinction of phoma lingam - using random primers of 8-11 nucleotide(s) giving differing pattern after gel-electrophoresis, useful in plant protection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
B. XUE ET AL.: "Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences", PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY, vol. 41, 1992, ACADEMIC PRESS LIMITED, NY,US;, pages 179 - 188 *
J.L. TAYLOR AND I.E. BORGMANN: "An unusual repetitive element from highly virulent isolates of Leptosphaeria maculans and evidence of its transfer to a weakly virulent isolate", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 7, no. 2, 1994, NATIONAL RESEARCH COUNCIL OF CANADA, CANADA;, pages 181 - 188 *
J.L. TAYLOR: "A simple, sensitive, and rapid method for detecting seed contaminated with highly virulent Leptoshaeria maculans", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 59, no. 11, November 1993 (1993-11-01), AM.SOC.MICROBIOL.,WASHINGTON,DC,US;, pages 3681 - 3685 *
W. MEYER ET AL.: "DNA- and PCR-fingerprinting in fungi", EXPERIMENTIA SUPPLEMENTUM (EXS), vol. 67, 1993, BIRKENHäUSER VERLAG, BASEL, SWITZERLAND, pages 311 - 320 *

Also Published As

Publication number Publication date
EP0689591A1 (en) 1996-01-03
CA2092115C (en) 1998-12-15
EP0689591B1 (en) 1997-08-06
WO1994021788A2 (en) 1994-09-29
CA2092115A1 (en) 1994-09-23
US5916744A (en) 1999-06-29

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