WO1995002067A1 - Improved method for the quantification of nucleic acid - Google Patents
Improved method for the quantification of nucleic acid Download PDFInfo
- Publication number
- WO1995002067A1 WO1995002067A1 PCT/EP1994/002295 EP9402295W WO9502067A1 WO 1995002067 A1 WO1995002067 A1 WO 1995002067A1 EP 9402295 W EP9402295 W EP 9402295W WO 9502067 A1 WO9502067 A1 WO 9502067A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- nucleic acid
- sample
- analyte
- amounts
- constructs
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
Definitions
- each construct being distinguishable from the analyte nucleic acid and capable of being co-amplified with the analyte nucleic acid
- reaction aliquots, each of a known volume, to each of which nucleic acid constructs are added in different ranges of amounts. Preferably, in that case, to each reaction volume the same nucleic acid constructs are added. If, for example, two reactions are used, a "low-range” reaction and a “high range” reaction can be carried out. For example, when the ranges of nucleic acid
- Contaminations with about 100-1000 molecules will slightly influence the internal calibration line and will only interfere with the calculation of the amount of analyte nucleic acid molecules present in the sample if the amount of analyte molecules in the sample is less than 1000 molecules. Contaminations with more than 1000 molecules will alter the calibration line and the outcome of the calculation.
- log (Q/A) log (Q/A)
- nucleic acid amplification procedure may be added, said quantity expected to be within the same range as the expected amount of analyte nucleic acid present in said sample, subjecting the sample to a nucleic acid amplification procedure, using
- pGEM3Q C ⁇ gag3 Q C -RNA
- SP6 RNA polymerase SP6 RNA polymerase
- the cloned inserts of pGEM3p24 and pGEM3Q A were recloned into vector pGEM4 creating pGEM4p24 and pGEM4Q A .
- Using these plasmids in vitro RNA was made using T7 RNA polymerase (Sambrook, 1989).
- the length of the in vitro RNA is 1514 nucleotides for plasmids pGEM3p24 (WT-RNA) and pGEM3Q A (Q A -RNA) and 1090 nucleotides for plasmids
- the number of amplification reactions can be decreased with the method according to the invention where several distinguishable internal standards are spiked into one amplification.
Abstract
Description
Claims
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU74928/94A AU668659B2 (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
EP94924750A EP0662156B1 (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
KR1019950700919A KR100312800B1 (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
DE69417863T DE69417863T2 (en) | 1993-07-09 | 1994-07-08 | IMPROVED METHOD FOR QUANTIFICATION OF NUCLEIC ACIDS |
CA002143857A CA2143857C (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
DK94924750T DK0662156T3 (en) | 1993-07-09 | 1994-07-08 | Improved method for quantifying nucleic acid |
JP50383695A JP3594598B2 (en) | 1993-07-09 | 1994-07-08 | Improved nucleic acid quantification method |
FI951082A FI112094B (en) | 1993-07-09 | 1995-03-08 | An improved method for quantifying nucleic acid |
GR990401631T GR3030554T3 (en) | 1993-07-09 | 1999-06-17 | Improved method for the quantification of nucleic acid. |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP93202015 | 1993-07-09 | ||
EP93202015.9 | 1993-07-09 | ||
EP93203424.2 | 1993-12-06 | ||
EP93203424 | 1993-12-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1995002067A1 true WO1995002067A1 (en) | 1995-01-19 |
Family
ID=26133903
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1994/002295 WO1995002067A1 (en) | 1993-07-09 | 1994-07-08 | Improved method for the quantification of nucleic acid |
Country Status (10)
Country | Link |
---|---|
JP (1) | JP3594598B2 (en) |
KR (1) | KR100312800B1 (en) |
AT (1) | ATE178946T1 (en) |
AU (1) | AU668659B2 (en) |
CA (1) | CA2143857C (en) |
DE (1) | DE69417863T2 (en) |
DK (1) | DK0662156T3 (en) |
ES (1) | ES2131699T3 (en) |
FI (1) | FI112094B (en) |
WO (1) | WO1995002067A1 (en) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0714988A2 (en) * | 1994-09-26 | 1996-06-05 | IMMUNO Aktiengesellschaft | Method for quantifying nucleic acids |
EP0722508A1 (en) * | 1993-09-22 | 1996-07-24 | Igen, Inc. | Self-sustained sequence replication electrochemiluminescent nucleic acid assay |
EP0747488A1 (en) * | 1995-06-07 | 1996-12-11 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1997012058A1 (en) * | 1995-09-26 | 1997-04-03 | Dynal A/S | Method for quantifying nucleic acid using multiple competitor nucleic acids |
US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1998000547A1 (en) * | 1996-07-03 | 1998-01-08 | Ambion, Inc. | Ribonuclease resistant rna preparation and utilization |
US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1998041650A2 (en) * | 1997-03-18 | 1998-09-24 | Visible Genetics Inc. | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
FR2765590A1 (en) * | 1997-07-01 | 1999-01-08 | Pasteur Institut | METHOD OF SEMI-QUANTIFICATION OF ALLELES |
US5919625A (en) * | 1996-07-03 | 1999-07-06 | Ambion, Inc. | Ribonuclease resistant viral RNA standards |
WO2000000638A2 (en) * | 1998-06-26 | 2000-01-06 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
EP1013776A1 (en) * | 1998-12-22 | 2000-06-28 | Universiteit van Amsterdam | A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid |
WO2000046401A1 (en) * | 1999-02-03 | 2000-08-10 | Lgc (Teddington) Limited | Reference material for nucleic acid amplification |
WO2001007661A2 (en) * | 1999-07-23 | 2001-02-01 | Gen-Probe Incorporated | Polynucleotide amplification method |
WO2001046463A2 (en) * | 1999-12-22 | 2001-06-28 | Baxter Aktiengesellschaft | Methods for the preparation and use of internal standards for nucleic acid amplification assays |
EP1319716A1 (en) * | 2000-09-22 | 2003-06-18 | Hitachi, Ltd. | Method of analyzing nucleic acid |
EP1352091A2 (en) * | 2000-12-22 | 2003-10-15 | Cepheid | Compositions and methods enabling a totally internally controlled amplification reaction |
US7972778B2 (en) | 1997-04-17 | 2011-07-05 | Applied Biosystems, Llc | Method for detecting the presence of a single target nucleic acid in a sample |
WO2012114312A3 (en) * | 2011-02-25 | 2012-11-01 | Novartis Ag | Exogenous internal positive control |
US8889405B2 (en) | 2009-09-16 | 2014-11-18 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5564795B2 (en) * | 2009-01-06 | 2014-08-06 | 株式会社島津製作所 | DNA quantification method and gene analysis method |
KR102108855B1 (en) | 2017-10-30 | 2020-05-12 | 한국표준과학연구원 | Method for quantification of nucleic acids wherein stable isotope-labelled nucleic acids are used as internal standards and uses thereof |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0303459A2 (en) * | 1987-08-11 | 1989-02-15 | The President And Fellows Of Harvard College | Multiplex sequencing |
EP0525882A1 (en) * | 1991-08-02 | 1993-02-03 | Akzo Nobel N.V. | Quantification of nucleic acid |
WO1993010257A1 (en) * | 1991-11-15 | 1993-05-27 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Method for determining the quantity of a dna fragment of interest by enzymatic amplification of dna |
-
1994
- 1994-07-08 CA CA002143857A patent/CA2143857C/en not_active Expired - Lifetime
- 1994-07-08 KR KR1019950700919A patent/KR100312800B1/en not_active IP Right Cessation
- 1994-07-08 WO PCT/EP1994/002295 patent/WO1995002067A1/en active IP Right Grant
- 1994-07-08 AT AT94924750T patent/ATE178946T1/en active
- 1994-07-08 AU AU74928/94A patent/AU668659B2/en not_active Expired
- 1994-07-08 DK DK94924750T patent/DK0662156T3/en active
- 1994-07-08 JP JP50383695A patent/JP3594598B2/en not_active Expired - Lifetime
- 1994-07-08 ES ES94924750T patent/ES2131699T3/en not_active Expired - Lifetime
- 1994-07-08 DE DE69417863T patent/DE69417863T2/en not_active Expired - Lifetime
-
1995
- 1995-03-08 FI FI951082A patent/FI112094B/en not_active IP Right Cessation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0303459A2 (en) * | 1987-08-11 | 1989-02-15 | The President And Fellows Of Harvard College | Multiplex sequencing |
EP0525882A1 (en) * | 1991-08-02 | 1993-02-03 | Akzo Nobel N.V. | Quantification of nucleic acid |
WO1993010257A1 (en) * | 1991-11-15 | 1993-05-27 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Method for determining the quantity of a dna fragment of interest by enzymatic amplification of dna |
Non-Patent Citations (2)
Title |
---|
APOSTOLAKOS ET AL., ANALYTICAL BIOCHEMISTRY, vol. 213, September 1993 (1993-09-01), NEW YORK US, pages 277 - 284 * |
GEMEN ET AL., JOURNAL OF VIROLOGICAL METHODS, vol. 43, July 1993 (1993-07-01), AMSTERDAM NL, pages 177 - 188 * |
Cited By (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0722508A1 (en) * | 1993-09-22 | 1996-07-24 | Igen, Inc. | Self-sustained sequence replication electrochemiluminescent nucleic acid assay |
EP0722508A4 (en) * | 1993-09-22 | 1999-08-11 | Igen Inc | Self-sustained sequence replication electrochemiluminescent nucleic acid assay |
EP0714988A2 (en) * | 1994-09-26 | 1996-06-05 | IMMUNO Aktiengesellschaft | Method for quantifying nucleic acids |
EP0714988A3 (en) * | 1994-09-26 | 1997-04-16 | Immuno Ag | Method for quantifying nucleic acids |
US5789153A (en) * | 1994-09-26 | 1998-08-04 | Immuno Aktiengesellschaft | Method of quantitating nucleic acid |
EP0747488A1 (en) * | 1995-06-07 | 1996-12-11 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1996040990A1 (en) * | 1995-06-07 | 1996-12-19 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5705365A (en) * | 1995-06-07 | 1998-01-06 | Gen-Probe Incorporated | Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
AU709292B2 (en) * | 1995-06-07 | 1999-08-26 | Gen-Probe Incorporated | Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
US5710029A (en) * | 1995-06-07 | 1998-01-20 | Gen-Probe Incorporated | Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product |
WO1997012058A1 (en) * | 1995-09-26 | 1997-04-03 | Dynal A/S | Method for quantifying nucleic acid using multiple competitor nucleic acids |
US5919625A (en) * | 1996-07-03 | 1999-07-06 | Ambion, Inc. | Ribonuclease resistant viral RNA standards |
US6214982B1 (en) | 1996-07-03 | 2001-04-10 | Ambion Inc | Ribonuclease resistant RNA preparation and utilization |
US6399307B1 (en) | 1996-07-03 | 2002-06-04 | Ambion, Inc. | Methods of quantifying viral load in an animal with a ribonuclease resistant RNA preparation |
US7033749B2 (en) | 1996-07-03 | 2006-04-25 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
WO1998000547A1 (en) * | 1996-07-03 | 1998-01-08 | Ambion, Inc. | Ribonuclease resistant rna preparation and utilization |
US5939262A (en) * | 1996-07-03 | 1999-08-17 | Ambion, Inc. | Ribonuclease resistant RNA preparation and utilization |
WO1998041650A2 (en) * | 1997-03-18 | 1998-09-24 | Visible Genetics Inc. | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
WO1998041650A3 (en) * | 1997-03-18 | 1999-01-14 | Visible Genetics Inc | Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample |
US8859204B2 (en) | 1997-04-17 | 2014-10-14 | Applied Biosystems, Llc | Method for detecting the presence of a target nucleic acid sequence in a sample |
US8822183B2 (en) | 1997-04-17 | 2014-09-02 | Applied Biosystems, Llc | Device for amplifying target nucleic acid |
US8563275B2 (en) | 1997-04-17 | 2013-10-22 | Applied Biosystems, Llc | Method and device for detecting the presence of a single target nucleic acid in a sample |
US8551698B2 (en) | 1997-04-17 | 2013-10-08 | Applied Biosystems, Llc | Method of loading sample into a microfluidic device |
US8278071B2 (en) | 1997-04-17 | 2012-10-02 | Applied Biosystems, Llc | Method for detecting the presence of a single target nucleic acid in a sample |
US8257925B2 (en) | 1997-04-17 | 2012-09-04 | Applied Biosystems, Llc | Method for detecting the presence of a single target nucleic acid in a sample |
US9506105B2 (en) | 1997-04-17 | 2016-11-29 | Applied Biosystems, Llc | Device and method for amplifying target nucleic acid |
US8067159B2 (en) | 1997-04-17 | 2011-11-29 | Applied Biosystems, Llc | Methods of detecting amplified product |
US7972778B2 (en) | 1997-04-17 | 2011-07-05 | Applied Biosystems, Llc | Method for detecting the presence of a single target nucleic acid in a sample |
FR2765590A1 (en) * | 1997-07-01 | 1999-01-08 | Pasteur Institut | METHOD OF SEMI-QUANTIFICATION OF ALLELES |
WO1999001575A1 (en) * | 1997-07-01 | 1999-01-14 | Institut Pasteur De Lille | Method for semiquantization of alleles |
WO2000000638A2 (en) * | 1998-06-26 | 2000-01-06 | Akzo Nobel N.V. | Tagging of rna amplicons generated by transcription-based amplification |
WO2000000638A3 (en) * | 1998-06-26 | 2000-04-20 | Akzo Nobel Nv | Tagging of rna amplicons generated by transcription-based amplification |
EP1013776A1 (en) * | 1998-12-22 | 2000-06-28 | Universiteit van Amsterdam | A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid |
WO2000037678A1 (en) * | 1998-12-22 | 2000-06-29 | Universiteit Van Amsterdam | A sensitive assay for the detection or quantitation of human cytomegalovirus nucleic acid |
WO2000046401A1 (en) * | 1999-02-03 | 2000-08-10 | Lgc (Teddington) Limited | Reference material for nucleic acid amplification |
US6908735B2 (en) | 1999-07-23 | 2005-06-21 | Gen-Probe Incorporated | Polynucleotide quantitation method |
AU781570B2 (en) * | 1999-07-23 | 2005-06-02 | Gen-Probe Incorporated | Polynucleotide amplification method |
WO2001007661A2 (en) * | 1999-07-23 | 2001-02-01 | Gen-Probe Incorporated | Polynucleotide amplification method |
WO2001007661A3 (en) * | 1999-07-23 | 2002-07-11 | Gen Probe Inc | Polynucleotide amplification method |
WO2001046463A3 (en) * | 1999-12-22 | 2002-06-27 | Baxter Ag | Methods for the preparation and use of internal standards for nucleic acid amplification assays |
WO2001046463A2 (en) * | 1999-12-22 | 2001-06-28 | Baxter Aktiengesellschaft | Methods for the preparation and use of internal standards for nucleic acid amplification assays |
EP1319716A1 (en) * | 2000-09-22 | 2003-06-18 | Hitachi, Ltd. | Method of analyzing nucleic acid |
US7790368B1 (en) | 2000-09-22 | 2010-09-07 | Hitachi, Ltd. | Method for analyzing nucleic acid |
EP1319716A4 (en) * | 2000-09-22 | 2005-01-12 | Hitachi Ltd | Method of analyzing nucleic acid |
EP1352091A4 (en) * | 2000-12-22 | 2004-07-21 | Cepheid | Compositions and methods enabling a totally internally controlled amplification reaction |
EP1352091A2 (en) * | 2000-12-22 | 2003-10-15 | Cepheid | Compositions and methods enabling a totally internally controlled amplification reaction |
US8889405B2 (en) | 2009-09-16 | 2014-11-18 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
US9388467B2 (en) | 2009-09-16 | 2016-07-12 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
US9416418B2 (en) | 2009-09-16 | 2016-08-16 | Seiko Epson Corporation | Biochip and target DNA quantitative method |
WO2012114312A3 (en) * | 2011-02-25 | 2012-11-01 | Novartis Ag | Exogenous internal positive control |
US9580761B2 (en) | 2011-02-25 | 2017-02-28 | Novartis Ag | Exogenous internal positive control |
Also Published As
Publication number | Publication date |
---|---|
AU7492894A (en) | 1995-02-06 |
JPH08501222A (en) | 1996-02-13 |
AU668659B2 (en) | 1996-05-09 |
ATE178946T1 (en) | 1999-04-15 |
JP3594598B2 (en) | 2004-12-02 |
FI951082A (en) | 1995-03-08 |
DE69417863T2 (en) | 1999-10-07 |
KR100312800B1 (en) | 2002-02-28 |
CA2143857A1 (en) | 1995-01-19 |
FI951082A0 (en) | 1995-03-08 |
CA2143857C (en) | 2005-09-20 |
FI112094B (en) | 2003-10-31 |
DE69417863D1 (en) | 1999-05-20 |
DK0662156T3 (en) | 1999-10-25 |
ES2131699T3 (en) | 1999-08-01 |
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