WO1997004003A3 - Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts - Google Patents

Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts Download PDF

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Publication number
WO1997004003A3
WO1997004003A3 PCT/US1996/011581 US9611581W WO9704003A3 WO 1997004003 A3 WO1997004003 A3 WO 1997004003A3 US 9611581 W US9611581 W US 9611581W WO 9704003 A3 WO9704003 A3 WO 9704003A3
Authority
WO
WIPO (PCT)
Prior art keywords
stationary phase
salts
protein
buffer exchange
protein refolding
Prior art date
Application number
PCT/US1996/011581
Other languages
French (fr)
Other versions
WO1997004003A2 (en
Inventor
Robert J Seely
Michael R Ladisch
Original Assignee
Amgen Inc
Purdue Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc, Purdue Research Foundation filed Critical Amgen Inc
Priority to JP9506744A priority Critical patent/JPH11509551A/en
Priority to AU65444/96A priority patent/AU707719B2/en
Priority to EP96925295A priority patent/EP0840745A2/en
Priority to CA002227318A priority patent/CA2227318C/en
Publication of WO1997004003A2 publication Critical patent/WO1997004003A2/en
Publication of WO1997004003A3 publication Critical patent/WO1997004003A3/en
Priority to MXPA/A/1998/000520A priority patent/MXPA98000520A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Abstract

Disclosed is a quick and efficient refolding process which utilizes cellulosic rolled stationary phase to rapidly separate the reduced, denatured protein from the denaturant solution, thereby promoting high protein refold yields at higher protein concentrations, while significantly decreasing the volume needed to achieve protein refolding.
PCT/US1996/011581 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts WO1997004003A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP9506744A JPH11509551A (en) 1995-07-21 1996-07-11 A method for unwinding proteins by buffer exchange using a continuous stationary phase capable of separating proteins from salts
AU65444/96A AU707719B2 (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt
EP96925295A EP0840745A2 (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt
CA002227318A CA2227318C (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salt
MXPA/A/1998/000520A MXPA98000520A (en) 1995-07-21 1998-01-16 Process for reploying proteins through shock exchange using a continuous stationary phase capable of separating the proteins of the

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US50542095A 1995-07-21 1995-07-21
US08/505,420 1995-07-21

Publications (2)

Publication Number Publication Date
WO1997004003A2 WO1997004003A2 (en) 1997-02-06
WO1997004003A3 true WO1997004003A3 (en) 1997-02-27

Family

ID=24010237

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/011581 WO1997004003A2 (en) 1995-07-21 1996-07-11 Process for protein refolding by means of buffer exchange using a continuous stationary phase capable of separating proteins from salts

Country Status (6)

Country Link
EP (1) EP0840745A2 (en)
JP (1) JPH11509551A (en)
AU (1) AU707719B2 (en)
CA (1) CA2227318C (en)
WO (1) WO1997004003A2 (en)
ZA (1) ZA966151B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1627920A1 (en) * 2000-01-24 2006-02-22 Polymun Scientific Immunbiologische Forschung GmbH Method for the manufacture of recombinant trypsin
JP5229730B2 (en) * 2008-10-07 2013-07-03 独立行政法人農業・食品産業技術総合研究機構 Reactor module for solid-phase synthesizer and solid-phase synthesizer using the same

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0218374A1 (en) * 1985-09-06 1987-04-15 International Minerals And Chemical Corporation Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
WO1988008849A1 (en) * 1987-05-11 1988-11-17 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4965344A (en) * 1986-06-04 1990-10-23 Behringwerke Aktiengesellschaft Process for obtaining active proteins from a biologically inactive form
US4977248A (en) * 1985-03-29 1990-12-11 Creighton Thomas E Process for the production of a protein
WO1991007428A1 (en) * 1989-11-20 1991-05-30 Novo Nordisk A/S A protein purification method
EP0657466A1 (en) * 1993-11-10 1995-06-14 Pfizer Inc. A process for refolding of (pro-)chymosin, comprising recycling of urea

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4977248A (en) * 1985-03-29 1990-12-11 Creighton Thomas E Process for the production of a protein
EP0218374A1 (en) * 1985-09-06 1987-04-15 International Minerals And Chemical Corporation Purification and activation of proteins from insoluble inclusion bodies
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
US4965344A (en) * 1986-06-04 1990-10-23 Behringwerke Aktiengesellschaft Process for obtaining active proteins from a biologically inactive form
WO1988008849A1 (en) * 1987-05-11 1988-11-17 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
WO1991007428A1 (en) * 1989-11-20 1991-05-30 Novo Nordisk A/S A protein purification method
EP0657466A1 (en) * 1993-11-10 1995-06-14 Pfizer Inc. A process for refolding of (pro-)chymosin, comprising recycling of urea

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
HAMAKER K.H. ET AL.: "Chromatography for rapid buffer exchange and refolding of secretory leukocyte protease inhibitor", BIOTECHNOLOGY PROGRESS, vol. 12, no. 2, 1996, pages 184 - 189, XP000610750 *

Also Published As

Publication number Publication date
AU6544496A (en) 1997-02-18
CA2227318A1 (en) 1997-02-06
EP0840745A2 (en) 1998-05-13
AU707719B2 (en) 1999-07-15
ZA966151B (en) 1997-02-04
MX9800520A (en) 1998-05-31
CA2227318C (en) 2001-09-25
WO1997004003A2 (en) 1997-02-06
JPH11509551A (en) 1999-08-24

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