WO1999042620A3 - Articles of manufacture and methods for staining biomolecules - Google Patents

Articles of manufacture and methods for staining biomolecules Download PDF

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Publication number
WO1999042620A3
WO1999042620A3 PCT/US1999/003704 US9903704W WO9942620A3 WO 1999042620 A3 WO1999042620 A3 WO 1999042620A3 US 9903704 W US9903704 W US 9903704W WO 9942620 A3 WO9942620 A3 WO 9942620A3
Authority
WO
WIPO (PCT)
Prior art keywords
manufacture
biomolecules
articles
methods
staining
Prior art date
Application number
PCT/US1999/003704
Other languages
French (fr)
Other versions
WO1999042620A2 (en
WO1999042620A9 (en
Inventor
Jack G Chirikjian
Original Assignee
Edvotek
Jack G Chirikjian
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Edvotek, Jack G Chirikjian filed Critical Edvotek
Priority to CA002321100A priority Critical patent/CA2321100A1/en
Priority to AU27771/99A priority patent/AU2777199A/en
Priority to JP2000532558A priority patent/JP2002504351A/en
Priority to EP99908309A priority patent/EP1057001A2/en
Publication of WO1999042620A2 publication Critical patent/WO1999042620A2/en
Publication of WO1999042620A3 publication Critical patent/WO1999042620A3/en
Publication of WO1999042620A9 publication Critical patent/WO1999042620A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Abstract

Biomolecules contained in gels or on membranes can be stained or labeled, without direct contact with toxic staining solutions, by applying to the gel or membrane an article of manufacture comprising a flexible backing with a coating that contains a stain or label. During the application, the stain or label is transferred from the backing to the biomolecules, marking them. The volume of the staining solution in this procedure is minimized, and environmental hazards are reduced. Furthermore, ease of manufacture reduces the cost of the article.
PCT/US1999/003704 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules WO1999042620A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002321100A CA2321100A1 (en) 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules
AU27771/99A AU2777199A (en) 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules
JP2000532558A JP2002504351A (en) 1998-02-19 1999-02-19 Biomolecule staining product and method
EP99908309A EP1057001A2 (en) 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7517398P 1998-02-19 1998-02-19
US60/075,173 1998-02-19

Publications (3)

Publication Number Publication Date
WO1999042620A2 WO1999042620A2 (en) 1999-08-26
WO1999042620A3 true WO1999042620A3 (en) 1999-11-18
WO1999042620A9 WO1999042620A9 (en) 2000-03-02

Family

ID=22124035

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/003704 WO1999042620A2 (en) 1998-02-19 1999-02-19 Articles of manufacture and methods for staining biomolecules

Country Status (5)

Country Link
EP (1) EP1057001A2 (en)
JP (1) JP2002504351A (en)
AU (1) AU2777199A (en)
CA (1) CA2321100A1 (en)
WO (1) WO1999042620A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE428002T1 (en) 2003-09-30 2009-04-15 Molecular Probes Inc DETECTION OF IMMOBILIZED NUCLEIC ACID
WO2007010532A2 (en) * 2005-07-18 2007-01-25 Shmuel Bukshpan Compositions for one step colorimetric analysis and methods of using same

Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3678151A (en) * 1969-07-25 1972-07-18 Gugol Clini Tex Inc Biological staining method
DE2739008A1 (en) * 1977-08-30 1979-03-15 Behringwerke Ag Indicator sheet for use with liquids, esp. blood and urine - having coating of hydrophilic swellable polymer particles, preventing indicator running
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
EP0119622A2 (en) * 1983-03-17 1984-09-26 Fuji Photo Film Co., Ltd. Integral element for biological reaction and process for the preparation thereof
WO1987003965A1 (en) * 1985-12-18 1987-07-02 Celldynamics Ag Multi-indicator test strips for immuno-assays, their production and use
EP0246505A2 (en) * 1986-05-16 1987-11-25 Miles Inc. Process for the production of test strips by casting method
EP0274911A1 (en) * 1987-01-12 1988-07-20 Pall Corporation Diagnostic device, method for making, and method for using
US4839297A (en) * 1986-11-12 1989-06-13 Boehringer Mannheim Gmbh Test carrier and method for the analytical determination of a component of a body fluid
EP0320842A2 (en) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Method for binding biological reagents to porous supports
WO1990008840A1 (en) * 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
EP0387696A2 (en) * 1989-03-17 1990-09-19 Abbott Laboratories Method and device for improved reaction kinetics in nucleic acid hybridizations
WO1992007955A1 (en) * 1990-10-26 1992-05-14 Orion-Yhtymä Oy Method for evaluating the adequacy of clinical specimens for hybridization assays and kit for performing the evaluation
US5132439A (en) * 1990-11-19 1992-07-21 Promega Corporation Protein staining compositions and methods
WO1993013224A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Process for immobilizing nucleic acid probes on polystyrene surfaces
WO1993013405A1 (en) * 1991-12-23 1993-07-08 Tropix, Inc. Improved membrane for chemiluminescent blotting applications
US5316638A (en) * 1989-09-27 1994-05-31 Peter Jackson Treatment of carbohydrates
EP0677589A2 (en) * 1994-03-31 1995-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Method, test element and test kit for semi-quantitative detection of target nucleic acid
US5514501A (en) * 1994-06-07 1996-05-07 The United States Of America As Represented By The Secretary Of Commerce Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates
EP0745689A2 (en) * 1990-05-11 1996-12-04 Microprobe Corporation A dipstick for a nucleic acid hybridization assay
US5585276A (en) * 1991-05-10 1996-12-17 Wisconsin Alumni Research Foundation Medium and method for blotting macromolecules
US5641635A (en) * 1993-11-12 1997-06-24 Eastman Kodak Company Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix

Patent Citations (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3678151A (en) * 1969-07-25 1972-07-18 Gugol Clini Tex Inc Biological staining method
US4224277A (en) * 1975-04-11 1980-09-23 Boehringer Mannheim Gmbh Coated slides and apparatus for coating same
DE2739008A1 (en) * 1977-08-30 1979-03-15 Behringwerke Ag Indicator sheet for use with liquids, esp. blood and urine - having coating of hydrophilic swellable polymer particles, preventing indicator running
EP0119622A2 (en) * 1983-03-17 1984-09-26 Fuji Photo Film Co., Ltd. Integral element for biological reaction and process for the preparation thereof
WO1987003965A1 (en) * 1985-12-18 1987-07-02 Celldynamics Ag Multi-indicator test strips for immuno-assays, their production and use
EP0246505A2 (en) * 1986-05-16 1987-11-25 Miles Inc. Process for the production of test strips by casting method
US4839297A (en) * 1986-11-12 1989-06-13 Boehringer Mannheim Gmbh Test carrier and method for the analytical determination of a component of a body fluid
EP0274911A1 (en) * 1987-01-12 1988-07-20 Pall Corporation Diagnostic device, method for making, and method for using
EP0320842A2 (en) * 1987-12-18 1989-06-21 W.R. Grace & Co.-Conn. Method for binding biological reagents to porous supports
WO1990008840A1 (en) * 1989-02-03 1990-08-09 Eastman Kodak Company Nucleic acid test article and its use to detect a predetermined nucleic acid
EP0387696A2 (en) * 1989-03-17 1990-09-19 Abbott Laboratories Method and device for improved reaction kinetics in nucleic acid hybridizations
US5316638A (en) * 1989-09-27 1994-05-31 Peter Jackson Treatment of carbohydrates
EP0745689A2 (en) * 1990-05-11 1996-12-04 Microprobe Corporation A dipstick for a nucleic acid hybridization assay
WO1992007955A1 (en) * 1990-10-26 1992-05-14 Orion-Yhtymä Oy Method for evaluating the adequacy of clinical specimens for hybridization assays and kit for performing the evaluation
US5132439A (en) * 1990-11-19 1992-07-21 Promega Corporation Protein staining compositions and methods
US5585276A (en) * 1991-05-10 1996-12-17 Wisconsin Alumni Research Foundation Medium and method for blotting macromolecules
WO1993013224A1 (en) * 1991-12-23 1993-07-08 Chiron Corporation Process for immobilizing nucleic acid probes on polystyrene surfaces
WO1993013405A1 (en) * 1991-12-23 1993-07-08 Tropix, Inc. Improved membrane for chemiluminescent blotting applications
US5641635A (en) * 1993-11-12 1997-06-24 Eastman Kodak Company Dry elements, test devices, test kits and methods for chemiluminescent detection of analytes using peroxidase-labeled reagents
EP0677589A2 (en) * 1994-03-31 1995-10-18 Johnson & Johnson Clinical Diagnostics, Inc. Method, test element and test kit for semi-quantitative detection of target nucleic acid
US5514501A (en) * 1994-06-07 1996-05-07 The United States Of America As Represented By The Secretary Of Commerce Process for UV-photopatterning of thiolate monolayers self-assembled on gold, silver and other substrates
US5688642A (en) * 1994-12-01 1997-11-18 The United States Of America As Represented By The Secretary Of The Navy Selective attachment of nucleic acid molecules to patterned self-assembled surfaces
US5776684A (en) * 1995-12-28 1998-07-07 Edvotek Method for staining biomolecules using a gelled matrix

Also Published As

Publication number Publication date
JP2002504351A (en) 2002-02-12
AU2777199A (en) 1999-09-06
WO1999042620A2 (en) 1999-08-26
EP1057001A2 (en) 2000-12-06
WO1999042620A9 (en) 2000-03-02
CA2321100A1 (en) 1999-08-26

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