WO2000017637A1 - Method and system for producing a substance or a signal with coagulating or anticoagulant effect - Google Patents

Abstract

The invention concerns a method and a system for producing a signal, in particular an electric signal, or a substance having a coagulating or anticoagulant effect. The method is characterised in that it is based on a source substance with coagulating effect, in particular, Ca++ ions, or an anticoagulant effect, in particular heparin. The method consists in: transforming (10) the electromagnetic field derived from said source substance located in the chamber (D), into a signal, in particular an electric signal, using a transducer-receiver sensing the electromagnetic field; applying (12) to a receiving substance located in the chamber (E), in particular water or a water-ethanol mixture or homeopathic granules, said signal derived from said transducer-receiver, using a transducer-transmitter. After said treatment, the receiving substance, initially inactive, has a coagulating or anticoagulant effect.

Description

Method and system for producing a substance or signal having a coagulant or anticoagulant effect.

The present invention relates to a method and a system for producing a substance or a signal, in particular an electrical signal, having a coagulant or anticoagulant effect. The invention also relates to such a substance or such a signal and to their therapeutic effects. The invention also relates to a method and a system for testing the coagulant or anticoagulant effect of a substance or signal. We know, since the research work of Mr. Jacques Benveniste, especially those described in patent application WO 94/17406 published on August 4, 1994, that we can capture from an active biological and / or chemical element such that a chemical compound, a cell or a microorganism, or from a substance containing this active element, an "electromagnetic signal characteristic of the biological and / or chemical activity or of the biological and / or chemical behavior" of said substance and / or of said active element contained in said substance. It is also known that it is possible to transform, in particular by means of a transducer, such an electromagnetic signal into an electrical signal. "electrical signal characteristic of the biological and / or chemical activity or of the biological and / or chemical behavior of a substance or of an active element contained in said substance" any electrical signal riveted by scanning and / or signal processing. In this expression, "characteristic" is used in the sense that the physical parameters of the electrical signal are specific to the substance or to the active element contained in said substance. In other words, the application of this electrical signal, via a transducer, to a biological control system makes it possible: (i) to induce biological and / or chemical activity on said system biological control in relation to that of the original substance or of the active element which it contains,

(ii) to reveal a characteristic of the substance or of the active element which it contains, at the origin of said electrical signal. Patent application WO 94/17406, published on August 4, 1994, describes a method and a device for capturing "an electromagnetic signal characteristic of a biological and / or chemical activity or of a biological and / or chemical behavior" from of an active biological and / or chemical element such as a chemical compound, a cell or a microorganism, or from a substance containing this active element such as a purified preparation, a biological sample, a living being .

The inventors have since discovered that it is possible to improve the quality of the electromagnetic signal picked up as well as the reliability of the process for producing this signal and that it is therefore possible to produce a characteristic electrical signal capable of industrial applications.

These developments have been described in the French application

FR 98 12 058 filed on September 23, 1998. As necessary, the elements of this request, not yet published to date, useful for understanding the present invention, will be extracted and inserted in the present request.

Method and system according to the invention for producing a substance having a coagulant or anticoagulant effect.

The method according to the invention for producing a substance having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, comprises at least the steps following.

The purpose of step 1 is to transform the electromagnetic field originating from said source substance into a signal, in particular a signal electrical characteristic, by means of a transducer-receiver capturing said electromagnetic field.

The purpose of step 2 is to apply to a receiving substance, in particular water or a water-ethanol mixture or homeopathic granules, said signal coming from said transducer-receiver, by means of a transducer-transmitter.

It is noted that after the above-defined treatment, the receptor substance, initially inactive, exhibits coagulant or anticoagulant activity. The receptor substance thus treated will hereinafter be called the "treated substance".

The concentration of the active elements in the source substance, in particular the concentration of Ca ⁺⁺ ions having a coagulant effect or of heparin having an anticoagulant effect, can be of the order of 1 μM. It can also be very low and reach 10 ^"14 M. The source substance may also consist of homeopathic products, diluted if necessary in water for injection.

Preferably, to transform the electromagnetic field coming from said source substance into an electrical signal:

said source substance is placed in a zone subjected to an excitation field of an electrical, magnetic and / or electromagnetic nature and,

- The fields resulting from the interaction of the excitation field and said source substance are transformed into an electrical signal, by means of a transducer-receiver capturing said resulting fields. The system according to the invention for producing a substance having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, comprises at least the elements below defined. A transducer-receiver receives the electromagnetic field from of said source substance. Said transducer-receiver transforms said electromagnetic field into a signal, in particular an electrical signal. A transducer-transmitter makes it possible to apply the signal coming from said transducer-receiver to a receiving substance, in particular water or a water-ethanol mixture or homeopathic granules.

After the treatment implemented by the system defined above, the receptor substance, initially inactive, exhibits coagulant or anticoagulant activity. Preferably, the system according to the invention further comprises an emitter generating an excitation field of an electrical, magnetic and / or electromagnetic nature in an area where said source substance is located. A transducer-receiver, receiving the fields resulting from the interaction of said excitation field and said source substance, transforms said resulting fields into a signal, in particular an electrical signal. Substance according to the invention having a coagulant or anticoagulant effect

The invention also relates to a substance having a coagulant or anticoagulant effect. Said substance, in particular water or a water-ethanol mixture or homeopathic granules, is characterized in that it has been treated by means of an electrical or electromagnetic signal originating from a source substance having coagulant effects, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin. The invention also relates to the therapeutic applications of such a substance. The substance according to the invention can be used in the treatment of thromboembolic disease. It can also be used to carry out coagulation exploration tests.

Method according to the invention for testing the coagulant or anticoagulant effect of a substance

The invention also relates to a method for testing a substance having a coagulating effect, in particular Ca ^" ^ ions, or anticoagulant, including heparin. The method includes at least the following steps.

The purpose of step 1 is to transform the electromagnetic field originating from said substance, into a signal, in particular an electrical signal, by means of a transducer-receiver sensing said electromagnetic field.

The purpose of step 2 is to apply said signal from said transducer-receiver to a sensitive biological system, directly or indirectly. Preferably, according to the invention for transforming the electromagnetic field originating from said substance into an electrical signal:

said substance is placed in a zone subjected to an excitation field of an electrical, magnetic and / or electromagnetic nature,

- The fields resulting from the interaction of the excitation field and said source substance are transformed into an electrical signal, by means of a transducer-receiver capturing said resulting fields. Advantageously, the sensitive biological system can be blood or plasma to which the said signal is applied by means of a transducer-transmitter. One can also advantageously use plasma rich in platelets.

Advantageously, according to another alternative embodiment, the sensitive biological system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer- transmitter. The method according to the invention for testing the coagulant or anticoagulant effect of a substance can be applied to the control of production of homeopathic products.

Method and system according to the invention for producing a signal having a coagulant or anticoagulant effect. The method according to the invention for producing a signal, in particular an electrical or electromagnetic signal, having a coagulant or anticoagulant effect, from a source substance having a coagulant effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular of the heparin, comprises at least the step of transforming the electromagnetic field originating from said source substance, into a signal, in particular an electrical signal, by means of a transducer-receiver capturing said electromagnetic field.

Preferably, to transform the electromagnetic field coming from said source substance into an electrical signal:

said source substance is placed in an area subjected to an excitation field of an electrical, magnetic and / or electromagnetic nature,

- The fields resulting from the interaction of the excitation field and the source substance are transformed into a signal, in particular an electrical signal, by means of a transducer-receiver capturing said resulting fields.

Preferably also, the method according to the invention for producing a signal, in particular an electrical or electromagnetic signal, having a coagulant or anticoagulant effect, further comprises the step of checking the correlations between on the one hand, the signal coming from said transducer - receptor and on the other hand, the coagulating or anticoagulant activity of said source substance, by applying, directly or indirectly, said signal to a biological control system and by verifying that said biological control system reacts in accordance with the coagulating activity or anticoagulant of the source substance from which the signal comes. Advantageously, the biological control system is blood or plasma to which said signal is applied by means of a transducer-transmitter. It is also advantageous to use plasma rich in platelets.

Advantageously, in another alternative embodiment, the biological control system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer - transmitter.

The present invention also relates to a system for producing a signal, in particular an electrical or electromagnetic signal, having a coagulant or anticoagulant effect, from a source substance having a coagulant effect, in particular Ca ^" ^ ions, or anticoagulant, in particular of heparin. Said system comprises a transducer-receiver receiving the electromagnetic field originating from said source substance, said transducer-receiver transforming said electromagnetic field into a signal, in particular an electrical signal. Preferably, the system according to the invention further comprises an emitter generating an excitation field of an electrical, magnetic and / or electromagnetic nature in an area where said source substance is located, said transducer-receiver, receiving the fields resulting from the interaction of said excitation field and said source substance , transforms said resulting fields into a signal, in particular an electrical signal ique.

Preferably also, the system according to the invention further comprises control means for controlling the correlations between on the one hand, the signal coming from said transducer-receiver and on the other hand, the coagulant or anticoagulant activity of said source substance . Said control means comprise a transducer-transmitter applying, directly or indirectly, said signal to a biological control system. Said control means further comprise verification means for verifying that the biological control system reacts in accordance with the coagulant or anticoagulant activity of the source substance which is issued the signal.

Advantageously, the biological control system is blood or plasma to which said signal is applied by means of said transducer-transmitter. One can also advantageously use plasma rich in platelets.

Advantageously in another alternative embodiment, the biological control system is an animal, in particular a rabbit, to which a substance, in particular water, is administered, in particular under the tongue, treated by said signal by means of said transducer-transmitter. Signal according to the invention having a coagulant or anticoagulant effect

The present invention also relates to a signal proper, in particular an electrical or electromagnetic signal, having a coagulant or anticoagulant effect. Said signal is obtained from a source substance having a coagulating effect, in particular Ca ^" ^ ions, or anticoagulant, in particular heparin, by implementing the methods or the systems described above. Said signal is characterized in that a biological control system reacts, after direct or indirect application of said signal, in accordance with the coagulant or anticoagulant activity of the source substance from which the signal is derived. Advantageously, the biological control system is blood or plasma to which said signal is applied by means of a transducer-transmitter. It is also advantageous to use platelet-rich plasma. Advantageously in another variant embodiment, the biological control system is an animal, in particular a rabbit, to which one administers, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer-transmitter. s therapeutic applications of such a signal. The signal according to the invention can be used, directly or indirectly via a receptor material, in the treatment of thromboembolic diseases. It can also be used, directly or indirectly via a receiving material, to carry out tests for exploring coagulation. Method according to the invention for testing the coagulant or anticoagulant effect of a signal

The invention also relates to a method for testing a signal having a coagulant or anticoagulant effect. Said signal is obtained from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, by implementing the methods or systems previously described. The method according to the invention comprises the step of applying said signal, directly or indirectly, to a biological test system and of verifying that the biological test system reacts in accordance with the coagulant or anticoagulant activity of the source substance from which the signal.

Advantageously, the biological test system is blood or plasma to which said signal is applied by means of a transducer-transmitter. One can also advantageously use plasma rich in platelets. Advantageously, according to another alternative embodiment, the biological test system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer- transmitter.

The method according to the invention for testing the coagulant or anticoagulant effect of a signal can be applied to the control of production of homeopathic products.

Other characteristics and advantages of the invention will appear on reading the description of variant embodiments of the invention, given by way of non-limiting example, as well as on reading the examples of experiments which have enabled to validate the production process of a substance or characteristic electrical signal, having coagulant or anticoagulant effects. The description refers to the accompanying drawings in which:

- Figure 1 shows a diagram of an exemplary embodiment of a system for producing a characteristic electrical signal, and applying the characteristic electrical signal thus produced to a receptor substance or to a biological control system or to a system sensitive biological,

FIG. 1a represents a detailed perspective view of a part of the device for producing the electrical signal, showing the excitation field transmitter and the transducer-receiver receiving the resulting fields,

FIG. 1b represents in block diagram form the type of microcomputer used either to generate the excitation fields, or to record and transmit in digitized form the characteristic electrical signal,

- Figure le represents a detailed perspective view of part of a transducer-transmitter intended to apply the characteristic electrical signal to a receptor substance or to a biological control system or to a sensitive biological system.

General scheme of the system

We will now describe, with reference to FIGS. 1, 1a, 1b and 1c, an exemplary embodiment of a system making it possible (i) to produce - from Ca ^" ^ ions a characteristic electrical signal having a coagulant effect, or

- from heparin a characteristic electrical signal having an anticoagulant effect and,

(ii) to apply such a characteristic signal to a substance receptor or a biological control system or a sensitive biological system.

The system comprises a device 10 for producing an electrical signal characteristic of the biological and / or chemical activity or of the biological and / or chemical behavior of a substance 1 or of an active element contained in said substance. In the case of the variant described with reference to FIGS. 1, la, lb and le, said substance 1 is: either Ca ⁺⁺ ions in solution at 1 μM in water for injectable preparation (for example of the Biosédra brand ), or heparin at a concentration of 2.5 IU / ml in the same quality of water.

The device 10, located in Paris for example, produces a characteristic electrical signal which is digitized after analog-digital conversion. The signal thus digitized is, in a manner known per se, transmitted remotely, for example by a computer communication network of the internet type using radio links 11. The digitized signal thus transmitted is received by an applicator 12, located in New York for example. The applicator 12 comprises transmission means 13. The transmission means 13 make it possible to apply the characteristic signal (after digital-analog conversion) to a receiving substance or to a biological control system or to a sensitive biological system. The means provided for digitizing and transmitting remotely the electrical signal characteristic of the Ca ⁺⁺ ion or of heparin are not essential to the realization of the invention. They have been described to highlight the technical and commercial advantages linked to the possibility of producing an electrical signal characteristic of the Ca ⁺⁺ ion or of heparin having, like the source substances from which they originate, coagulant or anti coagulants. In the case of the variant described with reference to FIGS. 1, la, lb and le, - the receiving substance is water or a water-ethanol mixture or homeopathic granules,

- the biological control system or the sensitive biological system is blood or plasma. I The device producing the signal characteristic of the Ca ^ ion or heparin

• The enclosure

The signal production device 10 comprises an enclosure D, 2 provided with an electrical and magnetic shielding isolating it from the stray fields coming from the environment. The shielded cylindrical enclosure is made up of three superimposed layers: copper, soft iron, mumetal, made of sheet metal 1 mm thick. The enclosure has an internal diameter of 65 mm and a height of 100 mm. The enclosure is closed by a shielded cover 5. In the enclosure 2 is placed a tank 3 of glass or plastic having the dimension 10 mm x 10 mm x 45 mm. This tank 3 contains 1 ml of substance 1. That is to say: either Ca ⁺⁺ ions in solution at 1 μM in water for injection (for example of the Biosédra brand),

- or heparin at a concentration of 2.5 IU / ml in the same quality of water.

• The transmitter of the specific excitation field

A transmitter 4 is located inside the enclosure. It generates a specific excitation field of an electromagnetic nature. The transmitter is powered by a generator 14. The transmitter 4 comprises a coil advantageously supplemented by a soft iron magnetic core. The emitting coil 4 has an impedance of 300 ohms, an inside diameter of 6 mm, an outside diameter of 16 mm, a length of 6 mm. The soft iron magnetic core is placed in contact with the outer walls of the tank 3. Said substance 1 is thus subjected to the excitation field emitted by the transmitter 4. The generator 14 is designed to generate a low frequency signal in particular square or sinusoidal low frequency signals, pink noise or, advantageously, white noise. The spectrum of the excitation signal supplying the emitting coil 4 corresponds substantially to the spectrum of audible frequencies (20 Hz - 20,000 Hz). The generator 14 can be an analog signal generator of known type, using for example a read only memory (ROM, PROM, EPROM, EEPROM in English terminology) containing the digital signal of the desired noise. This memory is connected in a manner known per se to a digital-analog converter. One can also use a microcomputer 14 provided with a sound card 25 having a digital to analog converter 41. One can for example use a computer 14 of the PC type, operating under the WINDOWS ^® operating system 95 of the Company MICROSOFT and comprising, in addition to the sound card 25 a microprocessor 27, an input / output interface 29, a controller 31 of a mass memory 33 and a video interface 35 connected by one or more buses 37. The digital analog converter 41 of the sound card 25 has an output terminal 8. The output terminal 8 of the microcomputer sound card 14 is connected to the input terminal 8 'of the transmitter 4, via an amplifier 15 whose characteristics are the following: bandwidth from 10 Hz to 20 kHz, gain 1 to 10, input sensitivity +/- 1 V. Among the 25 sound cards that can be used, we can cite for example the Soundblaster 16 card sold by ^; CREATIVE LABS. • The transducer-receiver The transducer-receiver 6, located inside the enclosure 2, receives the fields resulting from the interaction of said specific excitation field and said substance 1. The transducer-receiver 6 transforms said fields resulting in an electrical signal. This electrical signal is present, at the output terminals 9 ′ of the transducer-receiver 6, in the form of a difference of variable potential or of an electric current of variable intensity. The transducer-receiver 6 comprises a coil having a soft iron core. This coil has an impedance of 300 ohms, an inside diameter of 6 mm, an outside diameter of 16 mm, a length of 6 mm. The soft iron magnetic core is placed in contact with the outer walls of the tank 3.

Advantageously, the characteristic electrical signal available at the output of the transducer-receiver 6 is amplified by an amplifier-preamplifier 16. The amplifier-preamplifier 16 has the following characteristics: bandwidth from 10 Hz to 20 kHz, gain 10 to

100, input sensitivity +/- 100 mV.

In the case of the variant embodiment described with reference to FIGS. 1, 1a, 1b, there is provided an excitation field transmitter 4. The use of such a transmitter 4 promotes the production of an electrical signal characteristic of the Ca ⁺⁺ ion or heparin. However, it is also possible to receive, by means of a transducer-receiver 6, a signal characteristic of the Ca ⁺⁺ ion or of heparin, without using an excitation field and without using a shielded enclosure. • Recording of the characteristic electrical signal • • Analog recording

The recording of the characteristic electrical signal, or that of the electrical signal which derives therefrom after amplification or processing, can be carried out in analog by a signal recorder, in particular on magnetic tape, adapted to the frequencies of the characteristic electrical signal at the output of the transducer. receiver 6. Since the bandwidth used corresponds to the audio band, it is possible in particular to use a tape recorder. The output terminal 9 'of the transducer-receiver 6 is connected to the microphone input or to the line input of such a tape recorder. During reading, the recorded characteristic electrical signal is collected at a output terminal, in particular at the line output or at the speaker output of the tape recorder.

• • Digital recording

Preferably, a digital recording of the characteristic electrical signal is carried out after analog-digital conversion of said signal. For this purpose, a microcomputer 17 is used, provided with a signal acquisition card 25. The microcomputer 17 further comprises a microprocessor 27, an input / output interface 29, a controller 31 of a mass memory 33 and a video 35 connected by one or more bus interface 37. it can for example use a PC type computer 17, operating under the WINDOWS ^® operating system 95 of the MICROSOFT Company This microcomputer can be of the same type as that used to generate the excitation field. It can be the same microcomputer. The output 9 'of the transducer-receiver 6 or of the amplifier-preamplifier 16 is connected to the input 9 of the analog-digital converter 39 of the card 25 of the computer 17. Preferably, the analog-digital converter 39 has a resolution higher than 12 bits. It is advantageously equal to 16 bits. Preferably also, the analog-digital converter 39 has a sampling frequency twice the maximum frequency that it is desired to be able to digitize, for example 44 kHz.

A characteristic electrical signal is acquired for a duration for example between 1 and 60 s (for example 6 sec) and the digital file is recorded in a mass memory 33, for example in the form of a sound file at WAV format. All connections are made with shielded cable. All devices are grounded.

• Characteristic electrical signal processing

For processing the characteristic electrical signal or the signal derived therefrom, the Matlab software from the company "The Math Works ".

The digital file, recorded as described above, may possibly undergo digital processing, such as for example digital amplification for signal level calibration, filtering for the elimination of unwanted frequencies, or be transformed into sound spectrum by a discrete FOURIER transform, preferably by the fast FOURIER transform algorithm (FTT in Anglo-Saxon teπninology). The duration of the signal produced can be increased by repeating a fragment or all of the file originally produced in a file several times.

Means for processing the characteristic electrical signal can be used to improve the performance of the characteristic electrical signal. In the case of a first alternative embodiment, a second transducer-receiver of the same type is provided as that previously described. In the absence of said substance, this second transducer-receiver transforms the excitation field into an electrical signal. This electrical signal is subtracted by a series connection opposite to the signal from the first transducer-receiver. This gives a characteristic electrical signal more representative of the interaction of the specific excitation field and the substance.

In the case of a second variant embodiment, the processing means take into account the characteristics of the specific excitation field and reprocess the characteristic electrical signal in the following manner. We first of all calculate the distribution of the spectral power (PSD). Then we truncate this spectral power while keeping only the frequency band going for example from 140 Hz to 14 kHz, we reconstitute a signal from this spectral power and neutral phases, for example randomly generated, finally we calibrate the power of the signal thus produced. By neutral phases, we mean phases not originating from a source substance exhibiting biological activity.

In the case of the variant embodiment described with reference to FIGS. 1, la, lb, le, provision is made to digitize, record and process the characteristic electrical signal before applying it to a receptor substance or a system biological control or to a sensitive biological system. These operations are not essential for the exploitation of the electrical signal characteristic of the Ca ⁺⁺ ion or of heparin, even if they favor the implementation thereof. The characteristic electrical signal available at the output of the transducer-receiver 6 and, where appropriate, of the preamplifier 16 already constitutes in itself a product capable of industrial applications. We will see below for which applications it can be implemented in particular by means of an applicator 12 making it possible to apply them to a receptor substance or to a biological control system or to a sensitive biological system.

IL Remote transmission of the characteristic electrical signal The file of the electrical signal characteristic of the Ca ⁺⁺ ion or of heparin, recorded in digital form as just described, possibly after processing, can be transferred remotely by a computer communication network. This network may include radio links 11. The file of the electrical signal characteristic of the Ca ⁺⁺ ion or of heparin, thus transmitted, is recorded by the mass memory of a microcomputer 18. It is possible, for example use a PC type computer, operating under the WINDOWS ^® 95 operating system from MICROSOFT. This microcomputer 18 can be of the same type as that used to generate the excitation field. The digitized characteristic electrical signal file, thus recorded by the remote microcomputer 18, can be used, in a manner known per se, to produce an analog characteristic electrical signal. The file, if any processed, is transformed by a digital-analog converter 41 of the card 25 (or of a separate card) of the computer 18. The digital-analog converter 41 delivers on its output 8 an analog electric signal characteristic of the biological activity Ca ⁺⁺ ion or the heparin from which it is derived. This analog electrical signal can be transformed, as will be described below, into an electromagnetic field and applied to a receptor substance or to a biological control system or to a sensitive biological system. read. The signal applicator characteristic of the ion Ca ^"" or heparin

A description will now be given, with reference to FIG. 1 a, of an embodiment of a system making it possible to apply the electrical signal characteristic of the Ca ⁺⁺ ion or of heparin to a receptive biological system and modify the chemical behavior. The container 50 contains the receptor biological system. In the case of the variant described with reference to FIGS. 1, la, lb and le, the container 50 contains:

- a receptor substance such as water or a water-ethanol mixture or homeopathic granules, or - a biological control system or a sensitive biological system such as blood or plasma.

The container 50 is placed in an electromagnetic field radiated by a transducer-transmitter 51, typically a coil. The coil has for example a length of 80 mm, an inside diameter of 50 mm, an outside diameter of 55 mm. It presents 300 turns of a wire of diameter

0.5 mm. Its impedance is 4 ohms. The coil 51 is connected to ground. Without this representing any limiting character, the coil 51 of the transducer-transmitter has a vertical axis allowing the introduction of the container 50 containing the receiving biological system. Terminals input 8 'of this coil 51 are connected, in the case of the embodiment described, to output 8 of the analog-digital converter 41 of the microcomputer 18 via an amplifier 19 having the following characteristics: bandwidth of 10 Hz to 20 kHz, gain 1 to 20, input sensitivity 250 mV, RMS 60W output power at 8 ohms, signal to noise ratio 80 dB. The voltage across the coil 51 has an amplitude of 10 Volts rms and the signal is applied for 10 min. The input terminals 8 ′ of the applicator can also be, in the case of certain variant embodiments, directly connected to the output of the preamplifier 16 or to the output 8 of the digital-analog converter

41 of the computer 17. Experiments In order to illustrate an alternative embodiment,

- a method and a system according to the invention for producing a substance having a coagulant or anticoagulant effect,

- a substance according to the invention having a coagulant or anticoagulant effect,

a method according to the invention for testing the coagulant or anticoagulant effect of a substance and its application to the production of homeopathic products,

- a method and a system according to the invention for producing a signal having a coagulant or anticoagulant effect,

- a signal according to the invention having a coagulant or anticoagulant effect - a method according to the invention for testing the coagulant / anticoagulant effect of a signal and its application to the production of homeopathic products, the following experiments we were carried out. Reminder of the effects of heparin and Ca ^→ ions on the coagulation of human or rabbit plasma

Heparin (25,000 IU / 5 ml, Choay Laboratory, Sanofi Winthrop) is an anticoagulant that works by inhibiting the transformation of prothrombin into thrombin. At the site of action, the effect of heparin is immediate. It acts through a natural inhibitor called a cofactor, or antithrombin III.

Protamine sulphate (10,000 IU / 10 ml, Choay Laboratory, Sanofi Winthrop) forms a salt with heparin and causes a unit for unit suppression of its anticoagulant effect. 1 ml of protamine solution neutralizes the anticoagulant activity of 1000 units of heparin.

The calcium ion Ca ^ is an essential ion for coagulation. Source substances and materials used

The characteristic electrical signals were recorded from samples of 1 ml of the following solutions: - Ca ⁺⁺ in solution at 1 μM in water for injection (for example of Biosédra brand)

- Mg ⁺⁺ in solution at 1 μM in the same quality of water,

- heparin in solution at a concentration of 2.5 IU / ml in the same quality of water, - heparin + protamine complex (respectively 2.5 IU / ml and

0.025 mg / ml), in solution in the same quality of water. The equipment used has been described with reference to Figures la, lb, le. The transducer-receiver 6 has the characteristics described. The transducer-transmitter 51, making it possible to apply the characteristic electrical signal to a receiving substance or to a biological control system or to a sensitive biological system, is an electromagnetic coil having the following characteristics:

- length: 80 mm,

- inside diameter: 50 mm, - number of turns: 300 turns,

- impedance: 4 ohms.

A coagulation assessment was made using the following notation: - significant coagulation: 2

- moderate coagulation: 1

- no coagulation: 0

Protocol No. 1. "In vitro" experiment: Coagulating or anticoagulant action of characteristic electrical signals on Platelet Rich Plasma (PRP).

The purpose of this protocol is to demonstrate that:

- on the one hand, the method and the system described make it possible to produce an electrical signal characteristic of the Ca ⁺⁺ ion and of heparin having respectively a coagulating or anticoagulant effect, and - on the other hand, the method and the described system make it possible to test an electrical signal having respectively a coagulating or anticoagulant effect.

As a biological control system for revealing the electrical signal characteristic of the Ca ⁺⁺ ion and heparin, or as a sensitive biological system for testing the coagulant or anticoagulant effect of an electrical signal, rabbit (or human). The blood of a "New-Zealand White" rabbit is taken from the artery of the ear and collected on an ACD anticoagulant (9 vol. Blood / 1 vol. ACD), the composition of which is as follows: citric acid 0.8% , sodium citrate 2.2%, anhydrous glucose 2.23%.

After centrifugation (180 g, 15 minutes) at room temperature, the blood is divided into 3 layers: from top to bottom, the Plasma Rich Plasma (PRP), the buffy coat and the pellet of red blood cells. The PRP is taken by pipette by gentle suction. Anticoagulant effect of a signal, anticoagulant effect of the electrical signal characteristic of heparin

5 ml of PRP are placed in a tube 50 in the center of an electromagnetic coil 51 to be exposed to the signal applied for 10 min with a voltage of 10V across the terminals of the coil.

1 ml samples of PRP thus treated are placed in four tubes.

20 μl of Ca ⁺⁺ (50, 100, 150 and 200 mM) are delivered in each tube to obtain final concentrations of calcium in the PRP of (1, 2, 3 and 4 mM). Then incubate for 15 to 20 minutes.

The results obtained are presented in the table below:

n: number of values; Avg: average; SD: standard deviation

It is observed that an application of the heparin signal has an effect of inhibiting the coagulation of the PRP. Under the same conditions, the PRP not exposed to a signal or the PRP exposed to a control signal, such as for example that of the heparin + protamine complex, does not have an inhibiting effect. This coagulation inhibiting effect is particularly notable for a Ca ⁺⁺ concentration of between 2 and 3 mM.

Thus, the biological control system constituted by plasma rich in platelets makes it possible to control that the characteristic signal of heparin has an anticoagulant effect. Thus, the sensitive biological system constituted by plasma rich in platelets makes it possible to test whether a characteristic signal has an anticoagulant effect.

Coagulating effect of a signal, coagulating effect of the electrical signal characteristic of the calcium ion (Ca ⁺⁺ )

1 ml of PRP is placed in a tube in the center of an electromagnetic coil to be exposed to the signal applied for 10 min with a voltage of 10 V across the coil.

The results obtained are presented in the table below

Interpretation

It is observed that an application of the Ca ⁺⁺ calcium signal has a PRP coagulation effect comparable to that of Ca ⁺⁺ calcium proper.

It is observed that an application of the signal of magnesium Mg ⁺⁺ does not induce any coagulation effect of the PRP.

Thus, the biological control system constituted by plasma rich in platelets makes it possible to control that the characteristic signal of calcium

Ca ^{++ has} a coagulating effect.

Thus, the sensitive biological system consisting of plasma rich in platelets allows us to test whether a characteristic signal has a coagulating effect.

Protocol 2. "In vivo" experience: Coagulating action or anticoagulant of characteristic electrical signals.

The purpose of this protocol is to demonstrate that:

- on the one hand, the process and the system described allow

* to produce an electrical signal characteristic of the Ca ⁺⁺ ion and of heparin, and

* to apply this electrical signal to a receiving substance having, after treatment, respectively a coagulating or anticoagulant effect, and

- on the other hand, the method and the system described make it possible to test a substance having respectively a coagulant or anticoagulant effect.

As a biological control system making it possible to reveal the coagulating or anticoagulant effect of the treated substance, or as a sensitive biological system making it possible to test the coagulating or anticoagulant effect of a substance, a rabbit is used which is administered, sublingually, treated water by means of an electrical signal characteristic of the source substance.

The water used is water for injection Biosédra in ampoules of 10 ml.

1. The water (10 ml) is placed in a tube 50 in the center of an electromagnetic coil 51. The water is exposed to the characteristic signal considered for 10 min with a voltage at the terminals of the coil of 10 V.

2. The water is then stirred for 15 seconds at the maximum speed of the vortex. 3. The rabbit is administered sublingually 1 ml of the water thus treated by the characteristic signal considered. Blood samples (1 ml) are taken from glass tubes at the artery of the ear, before administration, then 1, 5, 10, 15 and 30 minutes after administration of the treated water. The results obtained are presented in the table below

Interpretation

It is observed that an administration of water treated by the characteristic signal of heparin has an inhibiting effect on blood coagulation for fifteen minutes. On the other hand, administration of water treated by the signal of the heparin + protamine complex does not produce any inhibiting effect. Thus, the biological control system constituted by an animal makes it possible to control that a receptor substance treated by the characteristic signal of heparin, in particular of water, has an anticoagulant effect. Thus, the sensitive biological system constituted by an animal makes it possible to test, by controlling the characteristic signal of a substance (for example the heparin + protamine complex), whether this substance has a coagulant or anticoagulant effect.

It is thus thus established that one can control the production of homeopathic products by the use of substances with known effect (like heparin) and by controlling that the homeopathic products (granules, solutions, ...) from this substance also present, at the end of the chain, the corresponding activity (in the example described, the anticoagulation activity). The signal characteristic of a drug or a receptor substance treated by the signal characteristic of a drug has the same biological effects as the source drug of the signal considered.

Likewise, similar anticoagulant effects are obtained with hirudin on rabbit or human blood or plasma. Signals from hirudin have a stronger anticoagulant effect than those from heparin.

The results obtained with hirudin and rabbit blood are given below:

The results obtained with hirudin and human blood are given below:

Claims

claims

1. Method for producing a substance having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, said method comprising the steps:

transforming the electromagnetic field originating from said source substance into a signal, in particular an electrical signal, by means of a transducer-receiver sensing said electromagnetic field,

applying to a receiving substance, in particular water or a water-ethanol mixture or homeopathic granules, said signal coming from said transducer-receiver, by means of a transducer-transmitter,

(such that after the treatment defined above, the receptor substance, initially inactive, exhibits coagulant or anticoagulant activity; said receptor substance thus treated being hereinafter called the "treated substance").

2. Method according to claim 1, such as for transforming the electromagnetic field coming from said source substance into an electrical signal: - placing said source substance in an area subjected to an excitation field of electrical, magnetic and / or electromagnetic nature,

- The fields resulting from the interaction of the excitation field and said source substance are transformed into an electrical signal, by means of a transducer-receiver capturing said resulting fields.

3. System for producing a substance having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, said system comprising: - a transducer-receiver receiving the electromagnetic field originating from said source substance, said transducer-receiver transforming said electromagnetic field into a signal, in particular an electrical signal, - a transducer-transmitter for applying said signal originating from said transducer-receiver to a receiving substance , in particular water or a water-ethanol mixture or homeopathic granules, (so that after the treatment defined above, the receptor substance, initially inactive, has coagulant or anticoagulant activity; said receptor substance thus treated being hereinafter called the "treated substance").

4. System according to claim 3 such that it further comprises:

- an emitter generating an excitation field of an electrical, magnetic and / or electromagnetic nature in an area where said source substance, said transducer-receiver is located, receiving the fields resulting from the interaction of said excitation field and said substance source, transforming said resulting fields into a signal, in particular an electrical signal.

5. Substance having a coagulant or anticoagulant effect: the said substance

- which can in particular be water or a water-ethanol mixture or homeopathic granules, and

- having been treated by means of an electrical or electromagnetic signal coming from a source substance having coagulating effects, in particular Ca ⁺⁺ ions, or anticoagulants, in particular heparin.

6. Application of the substance according to claim 5:

- treatment of thromboembolic disease, or - exploring coagulation.

7. Method for testing a substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, said method comprising the steps: - of transforming the electromagnetic field coming from said substance, into a signal, in particular an electrical signal, by means of a transducer-receiver sensing said electromagnetic field,

- Apply to a sensitive biological system, directly or indirectly, said signal from said transducer-receiver.

8. The method as claimed in claim 7, such as for transforming the electromagnetic field originating from said substance into an electrical signal:

said substance is placed in a zone subjected to an excitation field of an electrical, magnetic and / or electromagnetic nature,

- The fields resulting from the interaction of the excitation field and said source substance are transformed into an electrical signal, by means of a transducer-receiver capturing said resulting fields.

9. Method according to any one of claims 7 or 8, such that the sensitive biological system is blood or plasma to which said signal is applied by means of a transducer-transmitter.

10. Method according to any one of claims 7 or 8, such that the sensitive biological system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer-transmitter.

11. Application of the method according to any one of claims 7 to 10 to the control of production of homeopathic products.

12. Method for producing a signal, in particular an electrical or electromagnetic signal, having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, said process comprising the steps:

transforming the electromagnetic field originating from said source substance into a signal, in particular an electrical signal, by means of a transducer-receiver sensing said electromagnetic field,

13. The method as claimed in claim 12, such as for transforming the electromagnetic field originating from said source substance into an electrical signal:

said source substance is placed in an area subjected to an excitation field of an electrical, magnetic and / or electromagnetic nature,

- The fields resulting from the interaction of the excitation field and the source substance are transformed into a signal, in particular an electrical signal, by means of a transducer-receiver capturing said resulting fields.

14. Method according to any one of claims 12 or 13, further comprising the step:

- to control the correlations between on the one hand, the signal coming from said transducer-receiver and on the other hand, the coagulant or anticoagulant activity of said source substance, by applying, directly or indirectly, said signal to a biological control system and verifying that said biological control system reacts in accordance with the coagulating or anticoagulant activity of the source substance from which the signal is derived.

15. The method of claim 14, such that the biological control system is blood or plasma to which said signal is applied by means of a transducer-transmitter.

16. The method of claim 14, such that the biological control system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated with said signal by means of a transducer-transmitter.

17. System for producing a signal, in particular an electrical or electromagnetic signal, having a coagulating or anticoagulant effect, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin, said system comprises a transducer-receiver receiving the electromagnetic field coming from said source substance, said transducer-receiver transforming said electromagnetic field into a signal, in particular an electrical signal,

18. The system of claim 17 as it further comprises:

- an emitter generating an excitation field of an electrical, magnetic and / or electromagnetic nature in an area where said source substance, said transducer-receiver is located, receiving the fields resulting from the interaction of said excitation field and said substance source, transforming said resulting fields into a signal, in particular an electrical signal.

19. System according to any one of claims 17 or 18, further comprising:

control means for controlling the correlations between on the one hand, the signal coming from said transducer-receiver and on the other hand, the coagulating or anticoagulant activity of said source substance, said control means comprising a transducer-transmitter applying, directly or indirectly, said signal to a biological control system, said control means further comprising verification means for verifying that the biological control system reacts in accordance with the coagulant or anticoagulant activity of the source substance from which the signal is derived .

20. The system of claim 19, such as the biological system of control is blood or plasma to which said signal is applied by means of said transducer-transmitter.

21. The system of claim 19, such that the biological control system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of said transducer. transmitter.

22. Signal, in particular electrical or electromagnetic signal, having a coagulating or anticoagulant effect, said signal being obtained by means of the method according to any one of claims 12 to 16 or of the system according to any one of claims 17 to 21, starting from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or anticoagulant, in particular heparin said signal being characterized in that a biological control system reacts, after direct or indirect application of said signal, in accordance the coagulating or anticoagulant activity of the source substance from which the signal is derived.

23. The signal as claimed in claim 22, such that the biological control system is blood or plasma to which said signal is applied by means of a transducer-transmitter.

24. The signal as claimed in claim 22, such that the biological control system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer-transmitter.

25. Application of the signal according to any one of claims 22 to

24, directly or indirectly via a receiving material,

- treatment of thromboembolic disease,

- exploring coagulation.

26. A method for testing a signal having a coagulating or anticoagulant effect, said signal being obtained by means of the method according to any one of claims 12 to 16 or by means of the system according to any one of claims 17 to 21, from a source substance having a coagulating effect, in particular Ca ⁺⁺ ions, or an anticoagulant, in particular heparin, said method comprising the step of applying said signal, directly or indirectly, to a biological test system and of verifying the test biological system reacts in accordance with the coagulant or anticoagulant activity of the source substance from which the signal is derived.

27. The method of claim 26, such that the test biological system is blood or plasma to which said signal is applied by means of a transducer-transmitter.

28. The method of claim 26, such that the biological test system is an animal, in particular a rabbit, to which is administered, in particular under the tongue, a substance, in particular water, treated by said signal by means of a transducer -transmitter.

29. Application of the method according to any one of claims 26 to 28 to the control of production of homeopathic products.