WO2000033082A3 - Methods for quantitation of hla-dr and cd11b - Google Patents

Methods for quantitation of hla-dr and cd11b Download PDF

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Publication number
WO2000033082A3
WO2000033082A3 PCT/US1999/028884 US9928884W WO0033082A3 WO 2000033082 A3 WO2000033082 A3 WO 2000033082A3 US 9928884 W US9928884 W US 9928884W WO 0033082 A3 WO0033082 A3 WO 0033082A3
Authority
WO
WIPO (PCT)
Prior art keywords
hla
quantitation
cd11b
kits
reagents
Prior art date
Application number
PCT/US1999/028884
Other languages
French (fr)
Other versions
WO2000033082A2 (en
Inventor
Kenneth A Davis
Original Assignee
Becton Dickinson Co
Kenneth A Davis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/204,860 external-priority patent/US6200766B1/en
Application filed by Becton Dickinson Co, Kenneth A Davis filed Critical Becton Dickinson Co
Priority to EP99964122A priority Critical patent/EP1135685B1/en
Priority to DE69932845T priority patent/DE69932845T2/en
Priority to JP2000585667A priority patent/JP2002531832A/en
Publication of WO2000033082A2 publication Critical patent/WO2000033082A2/en
Publication of WO2000033082A3 publication Critical patent/WO2000033082A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56977—HLA or MHC typing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70503—Immunoglobulin superfamily, e.g. VCAMs, PECAM, LFA-3
    • G01N2333/70539—MHC-molecules, e.g. HLA-molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70546—Integrin superfamily, e.g. VLAs, leuCAM, GPIIb/GPIIIa, LPAM
    • G01N2333/70553—Integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/826—Additives, e.g. buffers, diluents, preservatives

Abstract

Improved methods, reagents, and kits for quantitation of HLA-DR and/or CD11b expression on peripheral blood cells are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR and CD11b expression. Use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits can be used to assess immune competence, and to direct and monitor immunostimulatory therapies in patients exhibiting monocyte deactivation.
PCT/US1999/028884 1998-12-03 1999-12-02 Methods for quantitation of hla-dr and cd11b WO2000033082A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99964122A EP1135685B1 (en) 1998-12-03 1999-12-02 Methods for quantitation of hla-dr and cd11b
DE69932845T DE69932845T2 (en) 1998-12-03 1999-12-02 METHODS OF QUANTIFYING HLA-DR AND CD11B
JP2000585667A JP2002531832A (en) 1998-12-03 1999-12-02 Method for quantification of HLA-DR and CD11B

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/204,860 US6200766B1 (en) 1998-12-03 1998-12-03 Methods and reagents for quantitation of HLA-DR expression on peripheral blood cells
US09/204,860 1999-09-24
US09/406,013 US6423505B1 (en) 1998-12-03 1999-09-24 Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells
US09/406,013 1999-09-24

Publications (2)

Publication Number Publication Date
WO2000033082A2 WO2000033082A2 (en) 2000-06-08
WO2000033082A3 true WO2000033082A3 (en) 2000-11-30

Family

ID=26899858

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/028884 WO2000033082A2 (en) 1998-12-03 1999-12-02 Methods for quantitation of hla-dr and cd11b

Country Status (5)

Country Link
US (2) US6423505B1 (en)
EP (2) EP1135685B1 (en)
JP (1) JP2002531832A (en)
DE (1) DE69932845T2 (en)
WO (1) WO2000033082A2 (en)

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US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
AU2003280470A1 (en) * 2002-07-01 2004-01-19 Guava Technologies, Inc. Fluorescent dyes, energy transfer couples and methods
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
MXPA05001100A (en) 2002-08-01 2005-04-28 Xy Inc Low pressure sperm cell separation system.
MXPA05001654A (en) 2002-08-15 2005-10-18 Xy Inc High resolution flow cytometer.
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
MX347048B (en) 2003-03-28 2017-04-07 Inguran Llc * Apparatus, methods and processes for sorting particles and for providing sex-sorted animal sperm.
ES2541121T3 (en) 2003-05-15 2015-07-16 Xy, Llc Efficient classification of haploid cells by flow cytometry systems
CA2561661C (en) 2004-03-29 2015-11-24 Monsanto Technology Llc Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
AR049732A1 (en) 2004-07-22 2006-08-30 Monsanto Co PROCESS TO ENRICH A Sperm Cell Population
BRPI0609302A2 (en) 2005-04-15 2011-10-11 Becton Dickinson Co methods for predicting the development of sepsis and for diagnosing sepsis in an individual to be tested, microarray, kit for predicting the development of sepsis in an individual to be tested, computer program product, computer, computer system for determining if an individual is likely to develop sepsis, digital signal embedded in a carrier wave, and, graphical user interface to determine if an individual is likely to develop sepsis
JPWO2007136026A1 (en) * 2006-05-19 2009-10-01 忠和 鄭 Antigen protein quantification method and quantification kit
US7816135B2 (en) 2007-07-05 2010-10-19 Becton, Dickinson And Company Method of analyzing lymphocytes
US20090136945A1 (en) * 2007-10-10 2009-05-28 The Regents Of The University Of Michigan Compositions and methods for assessing disorders
US8669113B2 (en) 2008-04-03 2014-03-11 Becton, Dickinson And Company Advanced detection of sepsis
US8187885B2 (en) * 2009-05-07 2012-05-29 Nodality, Inc. Microbead kit and method for quantitative calibration and performance monitoring of a fluorescence instrument
CN104677810B (en) * 2015-01-30 2017-08-22 广东医学院附属医院 The detection kit and its application method of Basohil activation
EP3413717B1 (en) 2016-02-12 2021-07-28 Imerys Filtration Minerals, Inc. Method for killing arthropods
US20190086319A1 (en) * 2016-03-10 2019-03-21 Becton, Dickinson And Company Methods of evaluating a cellular sample for her-2/neu expression and compositions for practicing the same

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Also Published As

Publication number Publication date
WO2000033082A2 (en) 2000-06-08
US6951727B2 (en) 2005-10-04
EP1605263A2 (en) 2005-12-14
EP1605263A3 (en) 2006-12-06
EP1135685A2 (en) 2001-09-26
US6423505B1 (en) 2002-07-23
DE69932845D1 (en) 2006-09-28
US20020076734A1 (en) 2002-06-20
JP2002531832A (en) 2002-09-24
EP1135685B1 (en) 2006-08-16
DE69932845T2 (en) 2007-03-08

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