WO2000042210A3 - Microsatellite dna markers and uses thereof - Google Patents

Microsatellite dna markers and uses thereof Download PDF

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Publication number
WO2000042210A3
WO2000042210A3 PCT/US2000/000325 US0000325W WO0042210A3 WO 2000042210 A3 WO2000042210 A3 WO 2000042210A3 US 0000325 W US0000325 W US 0000325W WO 0042210 A3 WO0042210 A3 WO 0042210A3
Authority
WO
WIPO (PCT)
Prior art keywords
ssr
markers
primer pairs
loblolly pine
polymorphic
Prior art date
Application number
PCT/US2000/000325
Other languages
French (fr)
Other versions
WO2000042210A2 (en
Inventor
Craig S Echt
C Dana Nelson
Original Assignee
Int Paper Co
Us Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/232,785 external-priority patent/US6733965B2/en
Application filed by Int Paper Co, Us Agriculture filed Critical Int Paper Co
Priority to NZ512941A priority Critical patent/NZ512941A/en
Priority to AU24076/00A priority patent/AU2407600A/en
Publication of WO2000042210A2 publication Critical patent/WO2000042210A2/en
Publication of WO2000042210A3 publication Critical patent/WO2000042210A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Abstract

Microsatellite, simple sequence repeat (SSR), markers have much potential for enhancing genome mapping and genotype identification research in forest genetics and tree breeding. SSR markers were developed by isolating and sequencing 1539 loblolly pine SSR clones for 11 SSR motifs. After screening out redundancy among the sequences, 566 oligonucleotide PCR primer pairs flanking the SRRs were synthesized and evaluated for their ability to amplify genomic DNA from loblolly pine. The three SSR motifs that yielded the highest proportion of informative markers from sequenced clones were (AC)n, (AAAT)n, and (AAAC)n. Eighteen polymorphic tri-a nd tetranucleotide SSR loci were genotyped in 20 loblolly pine trees using automated fluorescent marker analysis. The average number of alleles per locus observed was 6.4, and the average polymorphism information content (PIC) was 0.547. Subsets of the 566 primer pairs were evaluated for their ability to amplify DNA from six other pine species, and 54 primer pairs amplified markers thatwere polymorphic among the species. The present invention also concerns the methods of using the identified SSR loci as genetic markers.
PCT/US2000/000325 1999-01-15 2000-01-06 Microsatellite dna markers and uses thereof WO2000042210A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
NZ512941A NZ512941A (en) 1999-01-15 2000-01-06 Length polymorphisms in microsatellite (simple sequence repeat - SSR) DNA markers and their use as genetic markers in pine
AU24076/00A AU2407600A (en) 1999-01-15 2000-01-06 Microsatellite dna markers and uses thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US23288499A 1999-01-15 1999-01-15
US09/232,884 1999-01-15
US09/232,785 US6733965B2 (en) 1999-01-15 1999-01-19 Microsatellite DNA markers and uses thereof
US09/232,785 1999-01-19

Publications (2)

Publication Number Publication Date
WO2000042210A2 WO2000042210A2 (en) 2000-07-20
WO2000042210A3 true WO2000042210A3 (en) 2007-06-14

Family

ID=26926330

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/000325 WO2000042210A2 (en) 1999-01-15 2000-01-06 Microsatellite dna markers and uses thereof

Country Status (3)

Country Link
AU (1) AU2407600A (en)
NZ (1) NZ512941A (en)
WO (1) WO2000042210A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005026260D1 (en) * 2004-03-01 2011-03-24 Immune Disease Inst Inc NATURAL IGM ANTIBODIES AND INHIBITORS THEREOF
AU2015202438B2 (en) * 2004-04-06 2017-05-25 Fibria Celulose S/A Cambium/xylem-preferred promoters and uses thereof
EP1591536A1 (en) * 2004-04-29 2005-11-02 Institut National de la Recherche Agronomique Method of selecting sunflower genotypes with high oleic acid content in seed oil
ES2351326B1 (en) * 2009-06-12 2011-12-13 Instituto Nacional De Investigaciones Y Tecnologia Agraria Y Alimentaria (Inia) METHOD FOR THE IDENTIFICATION OF THE ORIGIN OF COMMERCIAL SPROCKETS.
CN104293942B (en) * 2014-09-30 2016-04-20 中国科学院武汉植物园 For the SSR molecular marker A001 of Kiwifruit hybrid Population male and female sex identification
CN104278097B (en) * 2014-09-30 2016-01-06 中国科学院武汉植物园 For the SSR molecular marker A003 of Kiwifruit hybrid Population male and female sex identification
CN104313019B (en) * 2014-09-30 2017-05-03 中国科学院武汉植物园 SSR molecular marker A002 for identifying males and females of kiwi fruit hybrid populations
CN107557362B (en) * 2017-10-26 2019-08-23 南京林业大学 A kind of identification method of masson pine cpSSR polymorphism primer and its pine tree sibling species
CN108676907A (en) * 2018-06-04 2018-10-19 贵州师范大学 A method of it is sequenced based on transcript profile and obtains green hedge bavin SSR primers
CN115323060B (en) * 2021-06-11 2023-08-01 中国水产科学研究院珠江水产研究所 Fish nuclear gene molecular marker primer, molecular marker and molecular marker database
CN116179738B (en) * 2022-08-31 2023-09-22 中国医学科学院药用植物研究所海南分所 Core primer group for identifying SSR molecular markers of agilawood varieties and application
CN116200521B (en) * 2022-12-05 2023-08-18 东北林业大学 SSR (simple sequence repeat) marker primer group for identifying Korean pine clone and construction method and application of SSR marker primer group and fingerprint

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5378602A (en) * 1991-05-29 1995-01-03 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
US5721100A (en) * 1991-05-29 1998-02-24 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Three highly informative microsatellite repeat polymorphic DNA markers
US5843647A (en) * 1993-12-21 1998-12-01 University Of Leicester Simple tandem repeats

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5378602A (en) * 1991-05-29 1995-01-03 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
US5721100A (en) * 1991-05-29 1998-02-24 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Three highly informative microsatellite repeat polymorphic DNA markers
US5843647A (en) * 1993-12-21 1998-12-01 University Of Leicester Simple tandem repeats

Also Published As

Publication number Publication date
NZ512941A (en) 2003-09-26
WO2000042210A2 (en) 2000-07-20
AU2407600A (en) 2000-08-01

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