WO2001060247A1 - Generation of spatially-averaged excitation-emission map in heterogeneous tissue - Google Patents

Generation of spatially-averaged excitation-emission map in heterogeneous tissue Download PDF

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Publication number
WO2001060247A1
WO2001060247A1 PCT/US2001/005321 US0105321W WO0160247A1 WO 2001060247 A1 WO2001060247 A1 WO 2001060247A1 US 0105321 W US0105321 W US 0105321W WO 0160247 A1 WO0160247 A1 WO 0160247A1
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Prior art keywords
excitation
emission
fiber optic
radiation
dimensional
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PCT/US2001/005321
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French (fr)
Inventor
Wei D. Tian
Pierre Trepagnier
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Argose, Inc.
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Publication date
Application filed by Argose, Inc. filed Critical Argose, Inc.
Priority to EP01909293A priority Critical patent/EP1257192A1/en
Priority to AU2001237066A priority patent/AU2001237066A1/en
Priority to JP2001559348A priority patent/JP2003522578A/en
Priority to CA002400305A priority patent/CA2400305A1/en
Publication of WO2001060247A1 publication Critical patent/WO2001060247A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres

Definitions

  • This invention relates to the rapid ge' eration of in vivo tissue auto-fluorescence spectra, and in particular to methods and devices for the acquisition of two-dimensional fluorescence excitation-emission maps, useful in the evaluation of heterogeneous tissues.
  • Tissue fluorescence has been used extensively for various medical purposes, including diagnosing disease, such as cancer of the cervix, assessment of skin aging, and monitoring tissue analytes.
  • fluorescence spectra can only be completely characterized by studying the relationships between emission and excitation found on a two-dimensional excitation-emission map.
  • excitation-emission maps have been sparingly used until recently. Instead, spectra taken from a projection onto either the excitation or emission axes, i.e., only varying the excitation or emission wavelengths, have been gathered.
  • emission scans which involve excitation of the sample with a single wavelength and scanning the sample's emissions, have been popular because they can utilize a laser light source.
  • excitation scans require generating illumination over many wavelengths, and so are typically performed with a continuous-spectrum light source.
  • excitation scans require the use of sensitive photomultiplier detectors.
  • the present invention overcomes the problems and disadvantages associated with current strategies and designs and provides a device useful for analyzing heterogeneous tissues in vivo.
  • the present invention applies the technique of gathering an excitation-emission map simultaneously, using a two-dimensional CCD or similar photon detector, to in vivo tissue, while at the same time ameliorating the inhomogeneity problem that plagues in vivo fluorescence spectroscopy.
  • one embodiment of the invention is directed to a method for evaluating fluorescence of a heterogeneous tissue comprising the steps of exciting a two- dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths, collecting emission radiation from the two-dimensional portion of the tissue surface simultaneously with excitation, and forming a two- dimensional excitation-emission map of the excitation radiation and the simultaneously collected emission radiation and spatially averaging the excitation and emission radiation.
  • Another embodiment is directed to an instrument for evaluating fluorescence of a heterogeneous tissue comprising means for exciting a two- dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths, means for collecting emission radiation from the two- dimensional portion of the tissue surface simultaneously with excitation, and means for forming a two-dimensional excitation-emission map of the excitation radiation and the simultaneously collected emission radiation and spatially averaging the excitation and emission radiation.
  • Another embodiment is directed to a method of rapidly gathering UN and visible fluorescence spectra in vivo which have been spatially averaged over tissue, the method comprising the steps of illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths, collecting emission radiation simultaneously from the strips of tissue with the step of illuminating with the plurality of excitation wavelengths, and disposing the emission radiation onto a two-dimensional array of detector elements, wherein the two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in the map are collected at once.
  • Still another embodiment is directed to an instrument for rapidly gathering UV and visible fluorescence spectra in vivo which have been spatially averaged over tissue, the method comprising means for illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths, means for collecting emission radiation simultaneously from the strips of tissue with the step of illuminating with the plurality of excitation wavelengths, and means for disposing the emission radiation onto a two-dimensional array of detector elements, wherein the two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in the map are collected at once.
  • Figure 1 A schematic of an instrument of a preferred embodiment of the invention.
  • Figure 2 N schematic of the fiber optic heads of the instrument of Figure 1.
  • the present invention is directed to methods and devices for the acquisition of two-dimensional fluorescence excitation-emission maps, useful in the evaluation of non-homogenous tissues.
  • the present invention applies the technique of gathering an excitation-emission map simultaneously, using a two-dimensional CCD or similar photon detector, to in vivo tissue, while at the same time ameliorating the inhomogeneity problem that plagues in vivo fluorescence spectroscopy.
  • An important feature of the present invention is the spatial averaging of the excitation and emission radiation.
  • excitation was spread into a line so that each spectral region illuminated only a very small sample patch
  • a substantial strip or area of tissue is illuminated, thus spatially averaging over the sample.
  • this function is performed with a large fiber bundle, so that no moving parts are present.
  • the strip may be generated over time by sequentially scanning, using a moving mirror or other beam steering device.
  • a preferred embodiment of an instrument according to the invention is diagrammed in Figure 1, and the fiber optic heads of the instrument are shown in more detail in Figure 2.
  • three-dimensional fluorescence spectrometer 10 comprises a light source 12.
  • Light source 12 is preferably a UV-visible light source.
  • Light source 12 may be a continuous source with a shutter 14 or, more preferably, a flash lamp.
  • Excitation radiation at a plurality of excitation wavelengths from light source 12 passes through bandpass filter 16 where it strikes excitation grating 18. From excitation grating 18, the excitation radiation is directed through excitation lens 20 to excitation optical fiber head 22.
  • the excitation optics spectrally disperse the incident radiation and lead it to the excitation optical fiber head 22.
  • Excitation radiation then passes from excitation optical fiber head 22 via fiber optic bundle 24 to common fiber optic head 30. Excitation radiation from excitation fibers in common fiber optic head 30 is directed to the tissue or surface of interest 32.
  • Fluorescent radiation emitted from the tissue is then picked up by emission fibers in common fiber optic head 30.
  • the collected emission radiation is transmitted via emission fiber optic bundle 34 to emission fiber optic head 36.
  • emission fiber optic head 36 From emission fiber optic head 36, the collected radiation passes through emission lens 38 to the emission optics, i.e., emission grating 40. From emission grating 40, the collected radiation is sent to a two-dimensional CCD detector or similar photon detector 42.
  • the emission radiation is collected by a two-dimensional array of detector elements disposed in common fiber optic head 30.
  • the two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation- emission map in which all elements in the map are collected at once. Both the excitation and emission maps are gathered simultaneously using two-dimensional CCD 42. Spatial averaging of the excitation and emission radiation is then used to ameliorate any problems due to inhomogeneity.
  • Figure 2 depicts a detailed schematic of excitation fiber optic head 22, common fiber optic head 30 and emission fiber optic head 36 according to a preferred embodiment of the invention.
  • excitation fiber optic head 22 preferably measures 28 x 3 mm and comprises 15 fiber bundles 50, each fiber bundle containing 20 fibers.
  • Common fiber optic head 30 preferably measures 28 x 10 mm and comprises 15 linear mixed fiber arrays 52 from the excitation and emission bundles.
  • each linear array 52 contains 40 fibers, 20 excitation fibers and 20 emission fibers.
  • Emission fiber optic head 36 preferably measures 28 x 3 mm and comprises 15 fiber bundles 54.
  • each fiber bundle 50 preferably contains 20 optic fibers of 0.2 mm diameter, and collects narrowband light (bandwidth 8-20 nm) in a selected UV and visible wavelength region.
  • the excitation wavelengths are preferably 250-550 nm, more preferably, 270-450 nm, and most preferably, 270-390 nm.
  • the emission fibers are also arranged in 15 fiber bundles 54 comprising 20 fibers each at the emission end of the fiber bundle, arranged as the emission fibers.
  • each linear fiber array 52 (10 mm long, containing
  • each of alternating excitation and emission fibers delivers narrowband excitation light to a 10 mm strip of tissue. Different strips excite the tissue at different wavelengths and collect the fluorescence.
  • the relatively large area of common head reduces the problem of inhomogeneity of tissue site by spatially averaging.

Abstract

An instrument for evaluating fluorescence of a heterogeneous tissue includes means for exciting a two-dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths, means for collecting emission radiation from the two-dimensional portion of the tissue surface simultaneously with excitation of the portion, and means for forming a two-dimensional excitation-emission map of the excitation radiation and the simultaneously collected emission radiation and spatially averaging the excitation and emission radiation.

Description

GENERATION OF SPATIALLY-AVERAGED EXCITATION-EMISSION MAP IN HETEROGENEOUS TISSUE
Background of the Invention 1. Field of the Invention
This invention relates to the rapid ge' eration of in vivo tissue auto-fluorescence spectra, and in particular to methods and devices for the acquisition of two-dimensional fluorescence excitation-emission maps, useful in the evaluation of heterogeneous tissues. 2. Description of the Background
Tissue fluorescence has been used extensively for various medical purposes, including diagnosing disease, such as cancer of the cervix, assessment of skin aging, and monitoring tissue analytes.
A fluorescent species, i.e., fluorophore, will absorb incident light, and re-emit it at a longer wavelength. Therefore, unlike absorption spectroscopy, fluorescence spectra can only be completely characterized by studying the relationships between emission and excitation found on a two-dimensional excitation-emission map.
However, due to conventional instrumentation constraints, excitation-emission maps have been sparingly used until recently. Instead, spectra taken from a projection onto either the excitation or emission axes, i.e., only varying the excitation or emission wavelengths, have been gathered. For example, emission scans, which involve excitation of the sample with a single wavelength and scanning the sample's emissions, have been popular because they can utilize a laser light source. Further, excitation scans require generating illumination over many wavelengths, and so are typically performed with a continuous-spectrum light source. However, because the spectrum is recorded at a constant emission wavelength, excitation scans require the use of sensitive photomultiplier detectors.
Both emission and excitation scans, as well as synchronous scans, in which both excitation and excitation wavelengths are simultaneously incremented, have been extensively described in the literature. In addition, for homogeneous samples prepared in a cuvette, at least one commercial manufacturer offers an instrument which gathers an excitation-emission map at once in a cuvette. It does this by spreading the excitation beam along the length of the cuvette. The full spectrum excites any homogeneous sample. The resulting fluorescence is then collected and diffracted in the orthogonal direction. This process results in a two-dimensional excitation/emission image, which is collected with a two-dimensional CCD device.
This instrument depends upon sample homogeneity to work. However, in vivo tissues are quite inhomogeneous, and so would not be suitable for use with this instrument (even if taken ex vivo and placed in a cuvette). In fact, this inhomogeneity is a problem with in vivo fluorescence, as many spectra at different sites must be obtained and averaged in order to get a representative spectrum.
Consequently, there is a need for a device that can be used to gather and process fluorescent spectra from tissue in vivo, despite any inhomogeneity which may be present in the tissue. Summary of the Invention The present invention overcomes the problems and disadvantages associated with current strategies and designs and provides a device useful for analyzing heterogeneous tissues in vivo. The present invention applies the technique of gathering an excitation-emission map simultaneously, using a two-dimensional CCD or similar photon detector, to in vivo tissue, while at the same time ameliorating the inhomogeneity problem that plagues in vivo fluorescence spectroscopy.
Accordingly, one embodiment of the invention is directed to a method for evaluating fluorescence of a heterogeneous tissue comprising the steps of exciting a two- dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths, collecting emission radiation from the two-dimensional portion of the tissue surface simultaneously with excitation, and forming a two- dimensional excitation-emission map of the excitation radiation and the simultaneously collected emission radiation and spatially averaging the excitation and emission radiation. Another embodiment is directed to an instrument for evaluating fluorescence of a heterogeneous tissue comprising means for exciting a two- dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths, means for collecting emission radiation from the two- dimensional portion of the tissue surface simultaneously with excitation, and means for forming a two-dimensional excitation-emission map of the excitation radiation and the simultaneously collected emission radiation and spatially averaging the excitation and emission radiation.
Another embodiment is directed to a method of rapidly gathering UN and visible fluorescence spectra in vivo which have been spatially averaged over tissue, the method comprising the steps of illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths, collecting emission radiation simultaneously from the strips of tissue with the step of illuminating with the plurality of excitation wavelengths, and disposing the emission radiation onto a two-dimensional array of detector elements, wherein the two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in the map are collected at once.
Still another embodiment is directed to an instrument for rapidly gathering UV and visible fluorescence spectra in vivo which have been spatially averaged over tissue, the method comprising means for illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths, means for collecting emission radiation simultaneously from the strips of tissue with the step of illuminating with the plurality of excitation wavelengths, and means for disposing the emission radiation onto a two-dimensional array of detector elements, wherein the two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in the map are collected at once. Other embodiments and advantages of the invention are set forth in part in the description which follows, and in part, will be obvious from this description, or may be learned from the practice of the invention. Description of the Drawings Figure 1 A schematic of an instrument of a preferred embodiment of the invention. Figure 2 N schematic of the fiber optic heads of the instrument of Figure 1.
Description of the Invention
As embodied and broadly described herein, the present invention is directed to methods and devices for the acquisition of two-dimensional fluorescence excitation-emission maps, useful in the evaluation of non-homogenous tissues.
The present invention applies the technique of gathering an excitation-emission map simultaneously, using a two-dimensional CCD or similar photon detector, to in vivo tissue, while at the same time ameliorating the inhomogeneity problem that plagues in vivo fluorescence spectroscopy.
An important feature of the present invention is the spatial averaging of the excitation and emission radiation. In contrast to the prior art, in which excitation was spread into a line so that each spectral region illuminated only a very small sample patch, in the present invention a substantial strip or area of tissue is illuminated, thus spatially averaging over the sample.
In a preferred embodiment, this function is performed with a large fiber bundle, so that no moving parts are present. In alternate, less-preferred embodiments, the strip may be generated over time by sequentially scanning, using a moving mirror or other beam steering device. A preferred embodiment of an instrument according to the invention is diagrammed in Figure 1, and the fiber optic heads of the instrument are shown in more detail in Figure 2.
Referring to Figure 1, three-dimensional fluorescence spectrometer 10 comprises a light source 12. Light source 12 is preferably a UV-visible light source. Light source 12 may be a continuous source with a shutter 14 or, more preferably, a flash lamp. Excitation radiation at a plurality of excitation wavelengths from light source 12 passes through bandpass filter 16 where it strikes excitation grating 18. From excitation grating 18, the excitation radiation is directed through excitation lens 20 to excitation optical fiber head 22. As will be clear to those of skill in the art, the excitation optics spectrally disperse the incident radiation and lead it to the excitation optical fiber head 22.
Excitation radiation then passes from excitation optical fiber head 22 via fiber optic bundle 24 to common fiber optic head 30. Excitation radiation from excitation fibers in common fiber optic head 30 is directed to the tissue or surface of interest 32.
Fluorescent radiation emitted from the tissue is then picked up by emission fibers in common fiber optic head 30. The collected emission radiation is transmitted via emission fiber optic bundle 34 to emission fiber optic head 36. From emission fiber optic head 36, the collected radiation passes through emission lens 38 to the emission optics, i.e., emission grating 40. From emission grating 40, the collected radiation is sent to a two-dimensional CCD detector or similar photon detector 42.
The emission radiation is collected by a two-dimensional array of detector elements disposed in common fiber optic head 30. The two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation- emission map in which all elements in the map are collected at once. Both the excitation and emission maps are gathered simultaneously using two-dimensional CCD 42. Spatial averaging of the excitation and emission radiation is then used to ameliorate any problems due to inhomogeneity. Figure 2 depicts a detailed schematic of excitation fiber optic head 22, common fiber optic head 30 and emission fiber optic head 36 according to a preferred embodiment of the invention. Referring to Figure 2, excitation fiber optic head 22 preferably measures 28 x 3 mm and comprises 15 fiber bundles 50, each fiber bundle containing 20 fibers. Common fiber optic head 30 preferably measures 28 x 10 mm and comprises 15 linear mixed fiber arrays 52 from the excitation and emission bundles. Preferably, each linear array 52 contains 40 fibers, 20 excitation fibers and 20 emission fibers. Emission fiber optic head 36 preferably measures 28 x 3 mm and comprises 15 fiber bundles 54.
At the excitation end, each fiber bundle 50 preferably contains 20 optic fibers of 0.2 mm diameter, and collects narrowband light (bandwidth 8-20 nm) in a selected UV and visible wavelength region. The excitation wavelengths are preferably 250-550 nm, more preferably, 270-450 nm, and most preferably, 270-390 nm. There is 0.8 mm space between each fiber bundle to mitigate cross-talk. Thus, in the preferred embodiment, there are 300 fibers in each bundle.
The emission fibers are also arranged in 15 fiber bundles 54 comprising 20 fibers each at the emission end of the fiber bundle, arranged as the emission fibers. At the common end, each linear fiber array 52 (10 mm long, containing
20 each of alternating excitation and emission fibers) delivers narrowband excitation light to a 10 mm strip of tissue. Different strips excite the tissue at different wavelengths and collect the fluorescence.
The arrangement depicted in Figure 2 provides the following advantages:
1. The relatively large area of common head reduces the problem of inhomogeneity of tissue site by spatially averaging.
2. By using a 2-dimensional CCD array, the need for a moving detector system is eliminated. This speeds up collection as well as simplifies the design. 3. By collecting the spectra in parallel, rather than sequentially, UV exposure on the tissue is minimized.
Other embodiments and uses of the invention will be apparent to those skilled in the art from consideration of the specification and practice of the invention disclosed herein. All references cited herein, including all U.S. and foreign patents and patent applications, are specifically and entirely hereby incorporated herein by reference, including, but not limited to, U.S. Patent Application No. 09/287,486, filed April 6, 1999. U.S. Patent Application titled "Multivariate Analysis of Green to Ultraviolet Spectra of Cell and Tissue Samples," U.S. Patent Application titled "Reduction of Inter-Subject Variation Via Transfer Standardization," and U.S. Patent Application titled "Non-Invasive Tissue Glucose Level Monitoring," all filed contemporaneously herewith, are entirely and specifically incorporated by reference. It is intended that the specification and examples be considered exemplary only, with the true scope and spirit of the invention indicated by the following claims.

Claims

Claims
1. A method for evaluating fluorescence of a heterogeneous tissue comprising: exciting a two-dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths; collecting emission radiation from said two-dimensional portion of the tissue surface simultaneously with excitation of said portion; and forming a two-dimensional excitation-emission map of said excitation radiation and said simultaneously collected emission radiation and spatially averaging said excitation and emission radiation.
2. An instrument for evaluating fluorescence of a heterogeneous tissue comprising: means for exciting a two-dimensional portion of the tissue surface with excitation radiation at a plurality of excitation wavelengths; means for collecting emission radiation from said two-dimensional portion of the tissue surface simultaneously with excitation of said portion; and means for forming a two-dimensional excitation-emission map of said excitation radiation and said simultaneously collected emission radiation and spatially averaging said excitation and emission radiation.
3. A method of rapidly gathering UV and visible fluorescence spectra in vivo spatially averaged over tissue, said method comprising the steps of: illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths; collecting emission radiation simultaneously from said strips of tissue with the step of illuminating with said plurality of excitation wavelengths; and disposing said emission radiation onto a two-dimensional array of detector elements, wherein said two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in said map are collected at once.
4. An instrument for rapidly gathering UV and visible fluorescence spectra in vivo spatially averaged over tissue, said method comprising: means for illuminating strips of tissue with excitation radiation simultaneously at a plurality of excitation wavelengths; means for collecting emission radiation simultaneously from said strips of tissue with the step of illuminating with said plurality of excitation wavelengths; and means for disposing said emission radiation onto a two-dimensional array of detector elements, wherein said two-dimensional detector disposition is arranged by wavelength to form a two-dimensional excitation-emission map, in which all elements in said map are collected at once.
5. A common fiber optic head comprising: a plurality of excitation fiber optic bundles, wherein each excitation fiber optic bundle comprises one or more excitation fibers; a plurality of emission fiber optic bundles, wherein each emission fiber optic bundle comprises one or more emission fibers; and wherein distal ends of said fiber optic bundles are disposed in a two- dimensional array.
6. The common fiber optic head of claim 5, wherein said two-dimensional array consists of n rows and n columns, said distal ends of said excitation fiber optic bundles are disposed in all elements of every odd or even column, but not both, and said distal ends of said emission fiber optic bundles are disposed in all elements of every odd or even column, not occupied by said excitation fiber optic bundles.
7. The common fiber optic head of claim 5, wherein each of said fiber optic bundles contains 20 or more optic fibers.
8. The common fiber optic head of claim 6, wherein each of said excitation fibers carries excitation light having a wavelength between 250-550 nm.
9. The common fiber optic head of claim 8, wherein each column of said excitation fibers carries excitation light having a unique wavelength.
10. The common fiber optic head of claim 5, wherein said fiber optic bundles are spaced at least 0.5 mm apart.
11. The common fiber optic head of claim 5, wherein said emission fibers collect fluorescence emitted from a sample exposed to said excitation light.
12. A system comprising: said common fiber optic head of claim 5; an excitation radiation source; and an emission radiation detector.
13. The system of claim 12 further comprising means for generating a two-dimensional excitation and emission radiation map in which all elements in said map are gathered at once.
PCT/US2001/005321 2000-02-18 2001-02-20 Generation of spatially-averaged excitation-emission map in heterogeneous tissue WO2001060247A1 (en)

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EP01909293A EP1257192A1 (en) 2000-02-18 2001-02-20 Generation of spatially-averaged excitation-emission map in heterogeneous tissue
AU2001237066A AU2001237066A1 (en) 2000-02-18 2001-02-20 Generation of spatially-averaged excitation-emission map in heterogeneous tissue
JP2001559348A JP2003522578A (en) 2000-02-18 2001-02-20 Generation of spatially averaged excitation-emission maps in heterogeneous tissue
CA002400305A CA2400305A1 (en) 2000-02-18 2001-02-20 Generation of spatially-averaged excitation-emission map in heterogeneous tissue

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US60/183,345 2000-02-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11635381B2 (en) 2016-09-01 2023-04-25 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Method and device for measuring process parameters in liquid cultures

Families Citing this family (317)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5490505A (en) 1991-03-07 1996-02-13 Masimo Corporation Signal processing apparatus
MX9702434A (en) 1991-03-07 1998-05-31 Masimo Corp Signal processing apparatus.
US5638818A (en) 1991-03-21 1997-06-17 Masimo Corporation Low noise optical probe
US8019400B2 (en) 1994-10-07 2011-09-13 Masimo Corporation Signal processing apparatus
EP1905352B1 (en) 1994-10-07 2014-07-16 Masimo Corporation Signal processing method
US6002952A (en) 1997-04-14 1999-12-14 Masimo Corporation Signal processing apparatus and method
US6229856B1 (en) 1997-04-14 2001-05-08 Masimo Corporation Method and apparatus for demodulating signals in a pulse oximetry system
JP2002516689A (en) 1998-06-03 2002-06-11 マシモ・コーポレイション Stereo pulse oximeter
USRE41912E1 (en) 1998-10-15 2010-11-02 Masimo Corporation Reusable pulse oximeter probe and disposable bandage apparatus
US6721585B1 (en) 1998-10-15 2004-04-13 Sensidyne, Inc. Universal modular pulse oximeter probe for use with reusable and disposable patient attachment devices
US7245953B1 (en) 1999-04-12 2007-07-17 Masimo Corporation Reusable pulse oximeter probe and disposable bandage apparatii
US6463311B1 (en) 1998-12-30 2002-10-08 Masimo Corporation Plethysmograph pulse recognition processor
US6684090B2 (en) 1999-01-07 2004-01-27 Masimo Corporation Pulse oximetry data confidence indicator
US20020140675A1 (en) 1999-01-25 2002-10-03 Ali Ammar Al System and method for altering a display mode based on a gravity-responsive sensor
US6770028B1 (en) * 1999-01-25 2004-08-03 Masimo Corporation Dual-mode pulse oximeter
US6360114B1 (en) 1999-03-25 2002-03-19 Masimo Corporation Pulse oximeter probe-off detector
US6515273B2 (en) 1999-08-26 2003-02-04 Masimo Corporation System for indicating the expiration of the useful operating life of a pulse oximetry sensor
US6377829B1 (en) 1999-12-09 2002-04-23 Masimo Corporation Resposable pulse oximetry sensor
US6950687B2 (en) 1999-12-09 2005-09-27 Masimo Corporation Isolation and communication element for a resposable pulse oximetry sensor
US6430525B1 (en) 2000-06-05 2002-08-06 Masimo Corporation Variable mode averager
ATE549969T1 (en) 2000-08-18 2012-04-15 Masimo Corp PULSE OXIMETER WITH DUAL MODE
US20020075490A1 (en) * 2000-12-20 2002-06-20 Affymetrix, Inc. System and method for addressable light-directed microarray printing
US7139598B2 (en) * 2002-04-04 2006-11-21 Veralight, Inc. Determination of a measure of a glycation end-product or disease state using tissue fluorescence
US20070276199A1 (en) * 2002-04-04 2007-11-29 Ediger Marwood N Determination of a Measure of a Glycation End-Product or Disease State Using Tissue Fluorescence
FR2824139B1 (en) * 2001-04-27 2003-05-30 Commissariat Energie Atomique LUMINESCENCE MEASURING DEVICE WITH PREFILTRE EFFECT ELIMINATION
US6850787B2 (en) 2001-06-29 2005-02-01 Masimo Laboratories, Inc. Signal component processor
US6697658B2 (en) 2001-07-02 2004-02-24 Masimo Corporation Low power pulse oximeter
US7355512B1 (en) 2002-01-24 2008-04-08 Masimo Corporation Parallel alarm processor
US6850788B2 (en) 2002-03-25 2005-02-01 Masimo Corporation Physiological measurement communications adapter
JP3723845B2 (en) * 2002-03-26 2005-12-07 国立大学法人富山大学 Method and apparatus for measuring film thickness of organic thin film used in organic electroluminescence device
US8140147B2 (en) * 2002-04-04 2012-03-20 Veralight, Inc. Determination of a measure of a glycation end-product or disease state using a flexible probe to determine tissue fluorescence of various sites
US8131332B2 (en) * 2002-04-04 2012-03-06 Veralight, Inc. Determination of a measure of a glycation end-product or disease state using tissue fluorescence of various sites
US7486985B2 (en) * 2002-08-05 2009-02-03 Infraredx, Inc. Near-infrared spectroscopic analysis of blood vessel walls
EP1551299A4 (en) * 2002-08-05 2010-01-20 Infraredx Inc Near-infrared spectroscopic analysis of blood vessel walls
US6970792B1 (en) 2002-12-04 2005-11-29 Masimo Laboratories, Inc. Systems and methods for determining blood oxygen saturation values using complex number encoding
US7919713B2 (en) 2007-04-16 2011-04-05 Masimo Corporation Low noise oximetry cable including conductive cords
US6920345B2 (en) 2003-01-24 2005-07-19 Masimo Corporation Optical sensor including disposable and reusable elements
US7003338B2 (en) 2003-07-08 2006-02-21 Masimo Corporation Method and apparatus for reducing coupling between signals
US7500950B2 (en) 2003-07-25 2009-03-10 Masimo Corporation Multipurpose sensor port
US7254431B2 (en) 2003-08-28 2007-08-07 Masimo Corporation Physiological parameter tracking system
US7483729B2 (en) 2003-11-05 2009-01-27 Masimo Corporation Pulse oximeter access apparatus and method
US7438683B2 (en) 2004-03-04 2008-10-21 Masimo Corporation Application identification sensor
JP2007527776A (en) 2004-03-08 2007-10-04 マシモ・コーポレイション Physiological parameter system
CA2464029A1 (en) 2004-04-08 2005-10-08 Valery Telfort Non-invasive ventilation monitor
US20080319286A1 (en) * 2004-05-24 2008-12-25 Trent Ridder Optical Probes for Non-Invasive Analyte Measurements
US7594151B2 (en) * 2004-06-18 2009-09-22 Qualcomm, Incorporated Reverse link power control in an orthogonal system
US8452316B2 (en) 2004-06-18 2013-05-28 Qualcomm Incorporated Power control for a wireless communication system utilizing orthogonal multiplexing
US7197692B2 (en) 2004-06-18 2007-03-27 Qualcomm Incorporated Robust erasure detection and erasure-rate-based closed loop power control
US7532326B2 (en) * 2004-07-07 2009-05-12 Corcoran Timothy C Multiple-label fluorescence imaging using excitation-emission matrices
US7343186B2 (en) 2004-07-07 2008-03-11 Masimo Laboratories, Inc. Multi-wavelength physiological monitor
US9341565B2 (en) 2004-07-07 2016-05-17 Masimo Corporation Multiple-wavelength physiological monitor
US7937128B2 (en) 2004-07-09 2011-05-03 Masimo Corporation Cyanotic infant sensor
US8036727B2 (en) 2004-08-11 2011-10-11 Glt Acquisition Corp. Methods for noninvasively measuring analyte levels in a subject
US7254429B2 (en) 2004-08-11 2007-08-07 Glucolight Corporation Method and apparatus for monitoring glucose levels in a biological tissue
US20060189871A1 (en) 2005-02-18 2006-08-24 Ammar Al-Ali Portable patient monitor
ATE468808T1 (en) 2005-03-01 2010-06-15 Masimo Laboratories Inc NON-INVASIVE MULTIPARAMETER PATIENT MONITOR
US8848574B2 (en) 2005-03-15 2014-09-30 Qualcomm Incorporated Interference control in a wireless communication system
US8942639B2 (en) 2005-03-15 2015-01-27 Qualcomm Incorporated Interference control in a wireless communication system
WO2006101484A1 (en) * 2005-03-16 2006-09-28 Tisone, M., Carolyn Simultaneous recording of multispectral fluorescence signatures
EP1874178A4 (en) 2005-04-13 2009-12-09 Glucolight Corp Method for data reduction and calibration of an oct-based blood glucose monitor
US7962188B2 (en) 2005-10-14 2011-06-14 Masimo Corporation Robust alarm system
EP1941638A2 (en) 2005-10-27 2008-07-09 Qualcomm Incorporated Method and apparatus for estimating reverse link loading in a wireless communication system
JP5049289B2 (en) 2005-11-29 2012-10-17 マシモ コーポレイション Optical sensor including disposable and reusable elements
US7859668B2 (en) * 2005-12-15 2010-12-28 Honeywell International Inc. Apparatus and method for illuminator-independent color measurements
US7990382B2 (en) 2006-01-03 2011-08-02 Masimo Corporation Virtual display
US8182443B1 (en) 2006-01-17 2012-05-22 Masimo Corporation Drug administration controller
US20070244377A1 (en) * 2006-03-14 2007-10-18 Cozad Jenny L Pulse oximeter sleeve
US8219172B2 (en) 2006-03-17 2012-07-10 Glt Acquisition Corp. System and method for creating a stable optical interface
US7941199B2 (en) 2006-05-15 2011-05-10 Masimo Laboratories, Inc. Sepsis monitor
US8998809B2 (en) * 2006-05-15 2015-04-07 Cercacor Laboratories, Inc. Systems and methods for calibrating minimally invasive and non-invasive physiological sensor devices
US9176141B2 (en) 2006-05-15 2015-11-03 Cercacor Laboratories, Inc. Physiological monitor calibration system
WO2007140478A2 (en) 2006-05-31 2007-12-06 Masimo Corporation Respiratory monitoring
US10188348B2 (en) 2006-06-05 2019-01-29 Masimo Corporation Parameter upgrade system
US8442572B2 (en) * 2006-09-08 2013-05-14 Qualcomm Incorporated Method and apparatus for adjustments for delta-based power control in wireless communication systems
US8670777B2 (en) 2006-09-08 2014-03-11 Qualcomm Incorporated Method and apparatus for fast other sector interference (OSI) adjustment
US8457707B2 (en) 2006-09-20 2013-06-04 Masimo Corporation Congenital heart disease monitor
US8118620B2 (en) 2007-10-12 2012-02-21 Masimo Corporation Connector assembly with reduced unshielded area
USD609193S1 (en) 2007-10-12 2010-02-02 Masimo Corporation Connector assembly
US8315683B2 (en) 2006-09-20 2012-11-20 Masimo Corporation Duo connector patient cable
USD614305S1 (en) 2008-02-29 2010-04-20 Masimo Corporation Connector assembly
US9161696B2 (en) 2006-09-22 2015-10-20 Masimo Corporation Modular patient monitor
US8840549B2 (en) 2006-09-22 2014-09-23 Masimo Corporation Modular patient monitor
US20080081990A1 (en) * 2006-09-28 2008-04-03 The Research Foundation Of State University Of New York Apparatus, system, kit and method for heart mapping
US20080094228A1 (en) * 2006-10-12 2008-04-24 Welch James P Patient monitor using radio frequency identification tags
US8265723B1 (en) 2006-10-12 2012-09-11 Cercacor Laboratories, Inc. Oximeter probe off indicator defining probe off space
US9192329B2 (en) 2006-10-12 2015-11-24 Masimo Corporation Variable mode pulse indicator
JP2010506614A (en) 2006-10-12 2010-03-04 マシモ コーポレイション Perfusion index smoothing device
US9861305B1 (en) 2006-10-12 2018-01-09 Masimo Corporation Method and apparatus for calibration to reduce coupling between signals in a measurement system
US8255026B1 (en) 2006-10-12 2012-08-28 Masimo Corporation, Inc. Patient monitor capable of monitoring the quality of attached probes and accessories
US7880626B2 (en) 2006-10-12 2011-02-01 Masimo Corporation System and method for monitoring the life of a physiological sensor
US8600467B2 (en) 2006-11-29 2013-12-03 Cercacor Laboratories, Inc. Optical sensor including disposable and reusable elements
US8414499B2 (en) 2006-12-09 2013-04-09 Masimo Corporation Plethysmograph variability processor
US8852094B2 (en) 2006-12-22 2014-10-07 Masimo Corporation Physiological parameter system
US7791155B2 (en) 2006-12-22 2010-09-07 Masimo Laboratories, Inc. Detector shield
US8652060B2 (en) 2007-01-20 2014-02-18 Masimo Corporation Perfusion trend indicator
US8781544B2 (en) 2007-03-27 2014-07-15 Cercacor Laboratories, Inc. Multiple wavelength optical sensor
US8374665B2 (en) 2007-04-21 2013-02-12 Cercacor Laboratories, Inc. Tissue profile wellness monitor
US8764671B2 (en) 2007-06-28 2014-07-01 Masimo Corporation Disposable active pulse sensor
US8048040B2 (en) 2007-09-13 2011-11-01 Masimo Corporation Fluid titration system
US8310336B2 (en) 2008-10-10 2012-11-13 Masimo Corporation Systems and methods for storing, analyzing, retrieving and displaying streaming medical data
US8355766B2 (en) 2007-10-12 2013-01-15 Masimo Corporation Ceramic emitter substrate
US8274360B2 (en) 2007-10-12 2012-09-25 Masimo Corporation Systems and methods for storing, analyzing, and retrieving medical data
CN101889192B (en) * 2007-10-25 2012-07-04 纽约州立大学研究基金会 Single photon spectrometer
US8636670B2 (en) 2008-05-13 2014-01-28 The Invention Science Fund I, Llc Circulatory monitoring systems and methods
US20090287120A1 (en) 2007-12-18 2009-11-19 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Circulatory monitoring systems and methods
US9717896B2 (en) 2007-12-18 2017-08-01 Gearbox, Llc Treatment indications informed by a priori implant information
US9672471B2 (en) * 2007-12-18 2017-06-06 Gearbox Llc Systems, devices, and methods for detecting occlusions in a biological subject including spectral learning
US8280484B2 (en) 2007-12-18 2012-10-02 The Invention Science Fund I, Llc System, devices, and methods for detecting occlusions in a biological subject
WO2009111542A2 (en) 2008-03-04 2009-09-11 Glucolight Corporation Methods and systems for analyte level estimation in optical coherence tomography
WO2009134724A1 (en) 2008-05-02 2009-11-05 Masimo Corporation Monitor configuration system
US9107625B2 (en) 2008-05-05 2015-08-18 Masimo Corporation Pulse oximetry system with electrical decoupling circuitry
TR201901658T4 (en) 2008-05-20 2019-02-21 Univ Health Network EQUIPMENT AND METHOD FOR FLUORESCENT-BASED IMAGING AND MONITORING
US20100030040A1 (en) 2008-08-04 2010-02-04 Masimo Laboratories, Inc. Multi-stream data collection system for noninvasive measurement of blood constituents
USD621516S1 (en) 2008-08-25 2010-08-10 Masimo Laboratories, Inc. Patient monitoring sensor
EP2326239B1 (en) 2008-07-03 2017-06-21 Masimo Laboratories, Inc. Protrusion for improving spectroscopic measurement of blood constituents
US8203438B2 (en) 2008-07-29 2012-06-19 Masimo Corporation Alarm suspend system
SE532941C2 (en) 2008-09-15 2010-05-18 Phasein Ab Gas sampling line for breathing gases
WO2010031070A2 (en) 2008-09-15 2010-03-18 Masimo Corporation Patient monitor including multi-parameter graphical display
US8346330B2 (en) 2008-10-13 2013-01-01 Masimo Corporation Reflection-detector sensor position indicator
US8401602B2 (en) 2008-10-13 2013-03-19 Masimo Corporation Secondary-emitter sensor position indicator
US8771204B2 (en) 2008-12-30 2014-07-08 Masimo Corporation Acoustic sensor assembly
US8588880B2 (en) 2009-02-16 2013-11-19 Masimo Corporation Ear sensor
EP3605550A1 (en) 2009-03-04 2020-02-05 Masimo Corporation Medical monitoring system
US10032002B2 (en) 2009-03-04 2018-07-24 Masimo Corporation Medical monitoring system
US9323894B2 (en) 2011-08-19 2016-04-26 Masimo Corporation Health care sanitation monitoring system
US10007758B2 (en) 2009-03-04 2018-06-26 Masimo Corporation Medical monitoring system
US8388353B2 (en) 2009-03-11 2013-03-05 Cercacor Laboratories, Inc. Magnetic connector
US8897847B2 (en) 2009-03-23 2014-11-25 Masimo Corporation Digit gauge for noninvasive optical sensor
US8989831B2 (en) 2009-05-19 2015-03-24 Masimo Corporation Disposable components for reusable physiological sensor
US8571619B2 (en) 2009-05-20 2013-10-29 Masimo Corporation Hemoglobin display and patient treatment
US8418524B2 (en) 2009-06-12 2013-04-16 Masimo Corporation Non-invasive sensor calibration device
US8670811B2 (en) 2009-06-30 2014-03-11 Masimo Corporation Pulse oximetry system for adjusting medical ventilation
US8471713B2 (en) 2009-07-24 2013-06-25 Cercacor Laboratories, Inc. Interference detector for patient monitor
US8473020B2 (en) 2009-07-29 2013-06-25 Cercacor Laboratories, Inc. Non-invasive physiological sensor cover
US8688183B2 (en) 2009-09-03 2014-04-01 Ceracor Laboratories, Inc. Emitter driver for noninvasive patient monitor
US20110172498A1 (en) 2009-09-14 2011-07-14 Olsen Gregory A Spot check monitor credit system
US9579039B2 (en) 2011-01-10 2017-02-28 Masimo Corporation Non-invasive intravascular volume index monitor
DE112010003689T5 (en) 2009-09-17 2013-01-17 Marcelo Lamego Improved analyte monitoring using one or more accelerometers
US20110137297A1 (en) 2009-09-17 2011-06-09 Kiani Massi Joe E Pharmacological management system
US8571618B1 (en) 2009-09-28 2013-10-29 Cercacor Laboratories, Inc. Adaptive calibration system for spectrophotometric measurements
US20110082711A1 (en) 2009-10-06 2011-04-07 Masimo Laboratories, Inc. Personal digital assistant or organizer for monitoring glucose levels
US10463340B2 (en) 2009-10-15 2019-11-05 Masimo Corporation Acoustic respiratory monitoring systems and methods
US8755535B2 (en) 2009-10-15 2014-06-17 Masimo Corporation Acoustic respiratory monitoring sensor having multiple sensing elements
WO2011047211A1 (en) 2009-10-15 2011-04-21 Masimo Corporation Pulse oximetry system with low noise cable hub
US8523781B2 (en) 2009-10-15 2013-09-03 Masimo Corporation Bidirectional physiological information display
WO2011047216A2 (en) 2009-10-15 2011-04-21 Masimo Corporation Physiological acoustic monitoring system
US9066680B1 (en) 2009-10-15 2015-06-30 Masimo Corporation System for determining confidence in respiratory rate measurements
US9724016B1 (en) 2009-10-16 2017-08-08 Masimo Corp. Respiration processor
US9839381B1 (en) 2009-11-24 2017-12-12 Cercacor Laboratories, Inc. Physiological measurement system with automatic wavelength adjustment
WO2011069122A1 (en) 2009-12-04 2011-06-09 Masimo Corporation Calibration for multi-stage physiological monitors
US9153112B1 (en) 2009-12-21 2015-10-06 Masimo Corporation Modular patient monitor
WO2011091059A1 (en) 2010-01-19 2011-07-28 Masimo Corporation Wellness analysis system
JP2013521054A (en) 2010-03-01 2013-06-10 マシモ コーポレイション Adaptive alarm system
US8584345B2 (en) 2010-03-08 2013-11-19 Masimo Corporation Reprocessing of a physiological sensor
US9307928B1 (en) 2010-03-30 2016-04-12 Masimo Corporation Plethysmographic respiration processor
US9138180B1 (en) 2010-05-03 2015-09-22 Masimo Corporation Sensor adapter cable
US8712494B1 (en) 2010-05-03 2014-04-29 Masimo Corporation Reflective non-invasive sensor
US8666468B1 (en) 2010-05-06 2014-03-04 Masimo Corporation Patient monitor for determining microcirculation state
US9326712B1 (en) 2010-06-02 2016-05-03 Masimo Corporation Opticoustic sensor
US8740792B1 (en) 2010-07-12 2014-06-03 Masimo Corporation Patient monitor capable of accounting for environmental conditions
US9408542B1 (en) 2010-07-22 2016-08-09 Masimo Corporation Non-invasive blood pressure measurement system
WO2012027613A1 (en) 2010-08-26 2012-03-01 Masimo Corporation Blood pressure measurement system
US9775545B2 (en) 2010-09-28 2017-10-03 Masimo Corporation Magnetic electrical connector for patient monitors
WO2012050847A2 (en) 2010-09-28 2012-04-19 Masimo Corporation Depth of consciousness monitor including oximeter
US9211095B1 (en) 2010-10-13 2015-12-15 Masimo Corporation Physiological measurement logic engine
CA2814720C (en) * 2010-10-15 2016-12-13 Lockheed Martin Corporation Micro fluidic optic design
US8723677B1 (en) 2010-10-20 2014-05-13 Masimo Corporation Patient safety system with automatically adjusting bed
US20120226117A1 (en) 2010-12-01 2012-09-06 Lamego Marcelo M Handheld processing device including medical applications for minimally and non invasive glucose measurements
EP2673721A1 (en) 2011-02-13 2013-12-18 Masimo Corporation Medical characterization system
US9066666B2 (en) 2011-02-25 2015-06-30 Cercacor Laboratories, Inc. Patient monitor for monitoring microcirculation
US8830449B1 (en) 2011-04-18 2014-09-09 Cercacor Laboratories, Inc. Blood analysis system
US9095316B2 (en) 2011-04-20 2015-08-04 Masimo Corporation System for generating alarms based on alarm patterns
US9622692B2 (en) 2011-05-16 2017-04-18 Masimo Corporation Personal health device
US9986919B2 (en) 2011-06-21 2018-06-05 Masimo Corporation Patient monitoring system
US9532722B2 (en) 2011-06-21 2017-01-03 Masimo Corporation Patient monitoring system
US9245668B1 (en) 2011-06-29 2016-01-26 Cercacor Laboratories, Inc. Low noise cable providing communication between electronic sensor components and patient monitor
US11439329B2 (en) 2011-07-13 2022-09-13 Masimo Corporation Multiple measurement mode in a physiological sensor
US9192351B1 (en) 2011-07-22 2015-11-24 Masimo Corporation Acoustic respiratory monitoring sensor with probe-off detection
US8755872B1 (en) 2011-07-28 2014-06-17 Masimo Corporation Patient monitoring system for indicating an abnormal condition
US9782077B2 (en) 2011-08-17 2017-10-10 Masimo Corporation Modulated physiological sensor
WO2013056160A2 (en) 2011-10-13 2013-04-18 Masimo Corporation Medical monitoring hub
US9943269B2 (en) 2011-10-13 2018-04-17 Masimo Corporation System for displaying medical monitoring data
EP2765909B1 (en) 2011-10-13 2019-06-26 Masimo Corporation Physiological acoustic monitoring system
US9808188B1 (en) 2011-10-13 2017-11-07 Masimo Corporation Robust fractional saturation determination
US9778079B1 (en) 2011-10-27 2017-10-03 Masimo Corporation Physiological monitor gauge panel
US9445759B1 (en) 2011-12-22 2016-09-20 Cercacor Laboratories, Inc. Blood glucose calibration system
US11172890B2 (en) 2012-01-04 2021-11-16 Masimo Corporation Automated condition screening and detection
US9392945B2 (en) 2012-01-04 2016-07-19 Masimo Corporation Automated CCHD screening and detection
US10149616B2 (en) 2012-02-09 2018-12-11 Masimo Corporation Wireless patient monitoring device
US10307111B2 (en) 2012-02-09 2019-06-04 Masimo Corporation Patient position detection system
US9480435B2 (en) 2012-02-09 2016-11-01 Masimo Corporation Configurable patient monitoring system
WO2013148605A1 (en) 2012-03-25 2013-10-03 Masimo Corporation Physiological monitor touchscreen interface
JP6490577B2 (en) 2012-04-17 2019-03-27 マシモ・コーポレイション How to operate a pulse oximeter device
WO2013184965A1 (en) 2012-06-07 2013-12-12 Masimo Corporation Depth of consciousness monitor
US20130327956A1 (en) * 2012-06-07 2013-12-12 Achrolux Inc. Phosphor layer detection system
US9697928B2 (en) 2012-08-01 2017-07-04 Masimo Corporation Automated assembly sensor cable
US10827961B1 (en) 2012-08-29 2020-11-10 Masimo Corporation Physiological measurement calibration
US9955937B2 (en) 2012-09-20 2018-05-01 Masimo Corporation Acoustic patient sensor coupler
US9877650B2 (en) 2012-09-20 2018-01-30 Masimo Corporation Physiological monitor with mobile computing device connectivity
US9749232B2 (en) 2012-09-20 2017-08-29 Masimo Corporation Intelligent medical network edge router
US9717458B2 (en) 2012-10-20 2017-08-01 Masimo Corporation Magnetic-flap optical sensor
US9560996B2 (en) 2012-10-30 2017-02-07 Masimo Corporation Universal medical system
US9787568B2 (en) 2012-11-05 2017-10-10 Cercacor Laboratories, Inc. Physiological test credit method
US9750461B1 (en) 2013-01-02 2017-09-05 Masimo Corporation Acoustic respiratory monitoring sensor with probe-off detection
US9724025B1 (en) 2013-01-16 2017-08-08 Masimo Corporation Active-pulse blood analysis system
US9750442B2 (en) 2013-03-09 2017-09-05 Masimo Corporation Physiological status monitor
US10441181B1 (en) 2013-03-13 2019-10-15 Masimo Corporation Acoustic pulse and respiration monitoring system
US9965946B2 (en) 2013-03-13 2018-05-08 Masimo Corporation Systems and methods for monitoring a patient health network
US9986952B2 (en) 2013-03-14 2018-06-05 Masimo Corporation Heart sound simulator
WO2014158820A1 (en) 2013-03-14 2014-10-02 Cercacor Laboratories, Inc. Patient monitor as a minimally invasive glucometer
US9936917B2 (en) 2013-03-14 2018-04-10 Masimo Laboratories, Inc. Patient monitor placement indicator
US10456038B2 (en) 2013-03-15 2019-10-29 Cercacor Laboratories, Inc. Cloud-based physiological monitoring system
DE102013008003B4 (en) 2013-05-08 2015-03-19 Freshdetect Gmbh Measuring device for measuring a surface covering on a measuring object, in particular on a food, and its use
US9891079B2 (en) 2013-07-17 2018-02-13 Masimo Corporation Pulser with double-bearing position encoder for non-invasive physiological monitoring
US10555678B2 (en) 2013-08-05 2020-02-11 Masimo Corporation Blood pressure monitor with valve-chamber assembly
WO2015038683A2 (en) 2013-09-12 2015-03-19 Cercacor Laboratories, Inc. Medical device management system
WO2015054166A1 (en) 2013-10-07 2015-04-16 Masimo Corporation Regional oximetry pod
US11147518B1 (en) 2013-10-07 2021-10-19 Masimo Corporation Regional oximetry signal processor
US10828007B1 (en) 2013-10-11 2020-11-10 Masimo Corporation Acoustic sensor with attachment portion
US10832818B2 (en) 2013-10-11 2020-11-10 Masimo Corporation Alarm notification system
US10279247B2 (en) 2013-12-13 2019-05-07 Masimo Corporation Avatar-incentive healthcare therapy
US11259745B2 (en) 2014-01-28 2022-03-01 Masimo Corporation Autonomous drug delivery system
US10086138B1 (en) 2014-01-28 2018-10-02 Masimo Corporation Autonomous drug delivery system
US10532174B2 (en) 2014-02-21 2020-01-14 Masimo Corporation Assistive capnography device
US9924897B1 (en) 2014-06-12 2018-03-27 Masimo Corporation Heated reprocessing of physiological sensors
US10123729B2 (en) 2014-06-13 2018-11-13 Nanthealth, Inc. Alarm fatigue management systems and methods
US10231670B2 (en) 2014-06-19 2019-03-19 Masimo Corporation Proximity sensor in pulse oximeter
CN115919256A (en) 2014-07-24 2023-04-07 大学健康网络 Data collection and analysis for diagnostic purposes
US10111591B2 (en) 2014-08-26 2018-10-30 Nanthealth, Inc. Real-time monitoring systems and methods in a healthcare environment
WO2016036985A1 (en) 2014-09-04 2016-03-10 Masimo Corportion Total hemoglobin index system
US10383520B2 (en) 2014-09-18 2019-08-20 Masimo Semiconductor, Inc. Enhanced visible near-infrared photodiode and non-invasive physiological sensor
WO2016057553A1 (en) 2014-10-07 2016-04-14 Masimo Corporation Modular physiological sensors
EP3247439B8 (en) 2015-01-23 2021-03-03 Masimo Corporation Nasal/oral cannula system
US10205291B2 (en) 2015-02-06 2019-02-12 Masimo Corporation Pogo pin connector
USD755392S1 (en) 2015-02-06 2016-05-03 Masimo Corporation Pulse oximetry sensor
US10568553B2 (en) 2015-02-06 2020-02-25 Masimo Corporation Soft boot pulse oximetry sensor
KR20230170116A (en) 2015-02-06 2023-12-18 마시모 코오퍼레이션 Fold flex circuit for optical probes
US10524738B2 (en) 2015-05-04 2020-01-07 Cercacor Laboratories, Inc. Noninvasive sensor system with visual infographic display
WO2016191307A1 (en) 2015-05-22 2016-12-01 Cercacor Laboratories, Inc. Non-invasive optical physiological differential pathlength sensor
JP6505260B2 (en) * 2015-06-30 2019-04-24 アイメック・ヴェーゼットウェーＩｍｅｃ Ｖｚｗ Use of a radiation carrier in a radiation carrier and an optical sensor
US10448871B2 (en) 2015-07-02 2019-10-22 Masimo Corporation Advanced pulse oximetry sensor
CA2994172A1 (en) 2015-08-11 2017-02-16 Masimo Corporation Medical monitoring analysis and replay including indicia responsive to light attenuated by body tissue
CN113367671A (en) 2015-08-31 2021-09-10 梅西莫股份有限公司 Wireless patient monitoring system and method
US11504066B1 (en) 2015-09-04 2022-11-22 Cercacor Laboratories, Inc. Low-noise sensor system
US11679579B2 (en) 2015-12-17 2023-06-20 Masimo Corporation Varnish-coated release liner
US10993662B2 (en) 2016-03-04 2021-05-04 Masimo Corporation Nose sensor
US10537285B2 (en) 2016-03-04 2020-01-21 Masimo Corporation Nose sensor
US11191484B2 (en) 2016-04-29 2021-12-07 Masimo Corporation Optical sensor tape
WO2018009612A1 (en) 2016-07-06 2018-01-11 Patient Doctor Technologies, Inc. Secure and zero knowledge data sharing for cloud applications
US10617302B2 (en) 2016-07-07 2020-04-14 Masimo Corporation Wearable pulse oximeter and respiration monitor
EP3525661A1 (en) 2016-10-13 2019-08-21 Masimo Corporation Systems and methods for patient fall detection
US11504058B1 (en) 2016-12-02 2022-11-22 Masimo Corporation Multi-site noninvasive measurement of a physiological parameter
US10750984B2 (en) 2016-12-22 2020-08-25 Cercacor Laboratories, Inc. Methods and devices for detecting intensity of light with translucent detector
US10721785B2 (en) 2017-01-18 2020-07-21 Masimo Corporation Patient-worn wireless physiological sensor with pairing functionality
WO2018156809A1 (en) 2017-02-24 2018-08-30 Masimo Corporation Augmented reality system for displaying patient data
WO2018156648A1 (en) 2017-02-24 2018-08-30 Masimo Corporation Managing dynamic licenses for physiological parameters in a patient monitoring environment
US10327713B2 (en) 2017-02-24 2019-06-25 Masimo Corporation Modular multi-parameter patient monitoring device
US20180247712A1 (en) 2017-02-24 2018-08-30 Masimo Corporation System for displaying medical monitoring data
US11086609B2 (en) 2017-02-24 2021-08-10 Masimo Corporation Medical monitoring hub
US10388120B2 (en) 2017-02-24 2019-08-20 Masimo Corporation Localized projection of audible noises in medical settings
US11185262B2 (en) 2017-03-10 2021-11-30 Masimo Corporation Pneumonia screener
WO2018194992A1 (en) 2017-04-18 2018-10-25 Masimo Corporation Nose sensor
US10918281B2 (en) 2017-04-26 2021-02-16 Masimo Corporation Medical monitoring device having multiple configurations
USD835283S1 (en) 2017-04-28 2018-12-04 Masimo Corporation Medical monitoring device
USD835285S1 (en) 2017-04-28 2018-12-04 Masimo Corporation Medical monitoring device
USD835282S1 (en) 2017-04-28 2018-12-04 Masimo Corporation Medical monitoring device
KR102615025B1 (en) 2017-04-28 2023-12-18 마시모 코오퍼레이션 Spot check measurement system
USD835284S1 (en) 2017-04-28 2018-12-04 Masimo Corporation Medical monitoring device
JP7159208B2 (en) 2017-05-08 2022-10-24 マシモ・コーポレイション A system for pairing a medical system with a network controller by using a dongle
WO2019014629A1 (en) 2017-07-13 2019-01-17 Cercacor Laboratories, Inc. Medical monitoring device for harmonizing physiological measurements
USD906970S1 (en) 2017-08-15 2021-01-05 Masimo Corporation Connector
USD890708S1 (en) 2017-08-15 2020-07-21 Masimo Corporation Connector
KR102611362B1 (en) 2017-08-15 2023-12-08 마시모 코오퍼레이션 Waterproof connector for non-invasive patient monitors
KR20200074175A (en) 2017-10-19 2020-06-24 마시모 코오퍼레이션 Display configuration for medical monitoring systems
USD925597S1 (en) 2017-10-31 2021-07-20 Masimo Corporation Display screen or portion thereof with graphical user interface
WO2019089655A1 (en) 2017-10-31 2019-05-09 Masimo Corporation System for displaying oxygen state indications
US11766198B2 (en) 2018-02-02 2023-09-26 Cercacor Laboratories, Inc. Limb-worn patient monitoring device
WO2019204368A1 (en) 2018-04-19 2019-10-24 Masimo Corporation Mobile patient alarm display
WO2019209915A1 (en) 2018-04-24 2019-10-31 Cercacor Laboratories, Inc. Easy insert finger sensor for transmission based spectroscopy sensor
US10932729B2 (en) 2018-06-06 2021-03-02 Masimo Corporation Opioid overdose monitoring
US10779098B2 (en) 2018-07-10 2020-09-15 Masimo Corporation Patient monitor alarm speaker analyzer
US11872156B2 (en) 2018-08-22 2024-01-16 Masimo Corporation Core body temperature measurement
USD998631S1 (en) 2018-10-11 2023-09-12 Masimo Corporation Display screen or portion thereof with a graphical user interface
USD917550S1 (en) 2018-10-11 2021-04-27 Masimo Corporation Display screen or portion thereof with a graphical user interface
USD999246S1 (en) 2018-10-11 2023-09-19 Masimo Corporation Display screen or portion thereof with a graphical user interface
BR112021006841A2 (en) 2018-10-11 2021-07-13 Masimo Corporation non-invasive physiological sensor assembly and method of fabricating a connector assembly for a non-invasive sensor assembly
US11389093B2 (en) 2018-10-11 2022-07-19 Masimo Corporation Low noise oximetry cable
USD998630S1 (en) 2018-10-11 2023-09-12 Masimo Corporation Display screen or portion thereof with a graphical user interface
USD917564S1 (en) 2018-10-11 2021-04-27 Masimo Corporation Display screen or portion thereof with graphical user interface
USD916135S1 (en) 2018-10-11 2021-04-13 Masimo Corporation Display screen or portion thereof with a graphical user interface
US11406286B2 (en) 2018-10-11 2022-08-09 Masimo Corporation Patient monitoring device with improved user interface
USD897098S1 (en) 2018-10-12 2020-09-29 Masimo Corporation Card holder set
US11464410B2 (en) 2018-10-12 2022-10-11 Masimo Corporation Medical systems and methods
WO2020077149A1 (en) 2018-10-12 2020-04-16 Masimo Corporation System for transmission of sensor data using dual communication protocol
US11684296B2 (en) 2018-12-21 2023-06-27 Cercacor Laboratories, Inc. Noninvasive physiological sensor
US20210022628A1 (en) 2019-04-17 2021-01-28 Masimo Corporation Patient monitoring systems, devices, and methods
CN110221051B (en) * 2019-05-23 2021-06-22 南京航空航天大学 Dual-wavelength dual-scale nano-drug in-vivo monitoring system and time sequence control method
USD985498S1 (en) 2019-08-16 2023-05-09 Masimo Corporation Connector
USD921202S1 (en) 2019-08-16 2021-06-01 Masimo Corporation Holder for a blood pressure device
USD919094S1 (en) 2019-08-16 2021-05-11 Masimo Corporation Blood pressure device
USD919100S1 (en) 2019-08-16 2021-05-11 Masimo Corporation Holder for a patient monitor
USD917704S1 (en) 2019-08-16 2021-04-27 Masimo Corporation Patient monitor
US11832940B2 (en) 2019-08-27 2023-12-05 Cercacor Laboratories, Inc. Non-invasive medical monitoring device for blood analyte measurements
USD927699S1 (en) 2019-10-18 2021-08-10 Masimo Corporation Electrode pad
KR20220083771A (en) 2019-10-18 2022-06-20 마시모 코오퍼레이션 Display layouts and interactive objects for patient monitoring
US11879960B2 (en) 2020-02-13 2024-01-23 Masimo Corporation System and method for monitoring clinical activities
US11721105B2 (en) 2020-02-13 2023-08-08 Masimo Corporation System and method for monitoring clinical activities
WO2021188999A2 (en) 2020-03-20 2021-09-23 Masimo Corporation Health monitoring system for limiting the spread of an infection in an organization
USD933232S1 (en) 2020-05-11 2021-10-12 Masimo Corporation Blood pressure monitor
USD979516S1 (en) 2020-05-11 2023-02-28 Masimo Corporation Connector
USD980091S1 (en) 2020-07-27 2023-03-07 Masimo Corporation Wearable temperature measurement device
USD974193S1 (en) 2020-07-27 2023-01-03 Masimo Corporation Wearable temperature measurement device
USD946596S1 (en) 2020-09-30 2022-03-22 Masimo Corporation Display screen or portion thereof with graphical user interface
USD946597S1 (en) 2020-09-30 2022-03-22 Masimo Corporation Display screen or portion thereof with graphical user interface
USD946598S1 (en) 2020-09-30 2022-03-22 Masimo Corporation Display screen or portion thereof with graphical user interface
USD997365S1 (en) 2021-06-24 2023-08-29 Masimo Corporation Physiological nose sensor
USD1000975S1 (en) 2021-09-22 2023-10-10 Masimo Corporation Wearable temperature measurement device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5303026A (en) * 1991-02-26 1994-04-12 The Regents Of The University Of California Los Alamos National Laboratory Apparatus and method for spectroscopic analysis of scattering media
US5491344A (en) * 1993-12-01 1996-02-13 Tufts University Method and system for examining the composition of a fluid or solid sample using fluorescence and/or absorption spectroscopy
WO1997048331A1 (en) * 1996-06-19 1997-12-24 Board Of Regents, The University Of Texas System Method and apparatus for diagnosing squamous intraepithelial lesions of the cervix using fluorescence spectroscopy
WO1999057529A1 (en) * 1998-05-04 1999-11-11 The Board Of Regents Combined fluorescence and reflectance spectroscopy

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3652855A (en) * 1969-05-26 1972-03-28 John Armin Mcintyre Radiation image amplifier and display comprising a fiber optic matrix for detecting and coding the radiation image pattern
EP0063431B1 (en) 1981-04-10 1987-10-28 Masaaki Konomi Spectroscopic analyzer system
US5106387A (en) * 1985-03-22 1992-04-21 Massachusetts Institute Of Technology Method for spectroscopic diagnosis of tissue
GB2222770B (en) 1988-09-16 1992-07-29 Sandoz Ltd Pharmaceutical compositions containing cyclosporins
US5201318A (en) * 1989-04-24 1993-04-13 Rava Richard P Contour mapping of spectral diagnostics
US5569591A (en) 1990-08-03 1996-10-29 University College Of Wales Aberystwyth Analytical or monitoring apparatus and method
JPH06505183A (en) 1991-02-26 1994-06-16 マサチユセツツ・インスチチユート・オブ・テクノロジー Molecular spectrometer system and method for diagnosing tissues
US5370114A (en) 1992-03-12 1994-12-06 Wong; Jacob Y. Non-invasive blood chemistry measurement by stimulated infrared relaxation emission
IL107396A (en) 1992-11-09 1997-02-18 Boehringer Mannheim Gmbh Method and apparatus for analytical determination of glucose in a biological matrix
AU6186494A (en) 1993-05-07 1994-11-10 Diasense, Inc. Non-invasive determination of analyte concentration using non-continuous radiation
AU7828694A (en) 1993-08-24 1995-03-22 Mark R. Robinson A robust accurate non-invasive analyte monitor
US5599717A (en) 1994-09-02 1997-02-04 Martin Marietta Energy Systems, Inc. Advanced synchronous luminescence system
US5579773A (en) * 1994-09-30 1996-12-03 Martin Marietta Energy Systems, Inc. Laser-induced differential normalized fluorescence method for cancer diagnosis
US5697373A (en) * 1995-03-14 1997-12-16 Board Of Regents, The University Of Texas System Optical method and apparatus for the diagnosis of cervical precancers using raman and fluorescence spectroscopies
IL113333A (en) 1995-04-11 2001-01-28 Yoav Paltieli Optical-fiber type endoscope
AU1130797A (en) * 1995-08-24 1997-03-19 Purdue Research Foundation Fluorescence lifetime-based imaging and spectroscopy in tissues and other random media
US6240309B1 (en) * 1995-10-06 2001-05-29 Hitachi, Ltd. Optical measurement instrument for living body
US6232609B1 (en) 1995-12-01 2001-05-15 Cedars-Sinai Medical Center Glucose monitoring apparatus and method using laser-induced emission spectroscopy
US5725480A (en) 1996-03-06 1998-03-10 Abbott Laboratories Non-invasive calibration and categorization of individuals for subsequent non-invasive detection of biological compounds
CA2259254C (en) 1996-07-08 2008-02-19 Animas Corporation Implantable sensor and system for in vivo measurement and control of fluid constituent levels
US5876339A (en) * 1997-01-09 1999-03-02 Lemire; Robert Apparatus for optical breast imaging
EP1011426A1 (en) 1997-02-26 2000-06-28 Diasense, Inc. Individual calibration of blood glucose for supporting noninvasive self-monitoring blood glucose
US6124597A (en) 1997-07-07 2000-09-26 Cedars-Sinai Medical Center Method and devices for laser induced fluorescence attenuation spectroscopy
JP3758824B2 (en) 1997-08-06 2006-03-22 倉敷紡績株式会社 Biological pressure stabilization device
DE69836979T2 (en) 1997-11-12 2007-11-08 Lightouch Medical, Inc. METHOD FOR NON-INVASIVE ANALYTIC MEASUREMENT
US6370422B1 (en) * 1998-03-19 2002-04-09 Board Of Regents, The University Of Texas System Fiber-optic confocal imaging apparatus and methods of use
JP2002510515A (en) 1998-04-06 2002-04-09 ザ・ジェネラル・ホスピタル・コーポレイション Non-invasive tissue glucose level monitoring
US6505059B1 (en) 1998-04-06 2003-01-07 The General Hospital Corporation Non-invasive tissue glucose level monitoring
US6377842B1 (en) * 1998-09-22 2002-04-23 Aurora Optics, Inc. Method for quantitative measurement of fluorescent and phosphorescent drugs within tissue utilizing a fiber optic probe
US6157041A (en) 1998-10-13 2000-12-05 Rio Grande Medical Technologies, Inc. Methods and apparatus for tailoring spectroscopic calibration models
US6405065B1 (en) 1999-01-22 2002-06-11 Instrumentation Metrics, Inc. Non-invasive in vivo tissue classification using near-infrared measurements

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5303026A (en) * 1991-02-26 1994-04-12 The Regents Of The University Of California Los Alamos National Laboratory Apparatus and method for spectroscopic analysis of scattering media
US5491344A (en) * 1993-12-01 1996-02-13 Tufts University Method and system for examining the composition of a fluid or solid sample using fluorescence and/or absorption spectroscopy
WO1997048331A1 (en) * 1996-06-19 1997-12-24 Board Of Regents, The University Of Texas System Method and apparatus for diagnosing squamous intraepithelial lesions of the cervix using fluorescence spectroscopy
WO1999057529A1 (en) * 1998-05-04 1999-11-11 The Board Of Regents Combined fluorescence and reflectance spectroscopy

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JIJI RENEE D. ET AL.: "Excitation-emission matrix fluorescence based determination of carbamate pesticides and polycylic aromatic hydrocarbons", ANALYTICA CHIMICA ACTA, vol. 397, no. 1, 1999, pages 61 - 72, XP001006272 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11635381B2 (en) 2016-09-01 2023-04-25 Rheinisch-Westfälisch Technische Hochschule (RWTH) Aachen Method and device for measuring process parameters in liquid cultures

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