WO2002046435A3 - Engineered plasmids and their use for in situ production of genes - Google Patents

Engineered plasmids and their use for in situ production of genes Download PDF

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Publication number
WO2002046435A3
WO2002046435A3 PCT/US2001/047452 US0147452W WO0246435A3 WO 2002046435 A3 WO2002046435 A3 WO 2002046435A3 US 0147452 W US0147452 W US 0147452W WO 0246435 A3 WO0246435 A3 WO 0246435A3
Authority
WO
WIPO (PCT)
Prior art keywords
genes
situ production
nucleic acid
engineered plasmids
plasmid
Prior art date
Application number
PCT/US2001/047452
Other languages
French (fr)
Other versions
WO2002046435A2 (en
Inventor
Katherine S Bowdish
Shana Barbas-Frederickson
Ying-Chi Lin
Mark Renshaw
Martha Wild
John Mcwhirter
Original Assignee
Alexion Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc filed Critical Alexion Pharma Inc
Priority to AU2002226049A priority Critical patent/AU2002226049A1/en
Publication of WO2002046435A2 publication Critical patent/WO2002046435A2/en
Publication of WO2002046435A3 publication Critical patent/WO2002046435A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Abstract

Nucleic acid sequences encoding at least a portion of a polypeptide are directly incorporated into a plasmid by DNA polymerization or reverse transcription of a nucleic acid template. In particularly preferred embodiments, nucleic acid sequences encoding at least a portion of an antibody are directly incorporated into a plasmid by reverse transcription of messenger RNA (mRNA).
PCT/US2001/047452 2000-12-05 2001-12-05 Engineered plasmids and their use for in situ production of genes WO2002046435A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002226049A AU2002226049A1 (en) 2000-12-05 2001-12-05 Engineered plasmids and their use for in situ production of genes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25144000P 2000-12-05 2000-12-05
US60/251,440 2000-12-05

Publications (2)

Publication Number Publication Date
WO2002046435A2 WO2002046435A2 (en) 2002-06-13
WO2002046435A3 true WO2002046435A3 (en) 2003-06-12

Family

ID=22951980

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/047452 WO2002046435A2 (en) 2000-12-05 2001-12-05 Engineered plasmids and their use for in situ production of genes

Country Status (3)

Country Link
US (2) US20030049731A1 (en)
AU (1) AU2002226049A1 (en)
WO (1) WO2002046435A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002046436A2 (en) * 2000-12-08 2002-06-13 Alexion Pharmaceuticals, Inc. Plasmid vectors
FR2924440B1 (en) * 2007-12-04 2015-01-09 Pf Medicament NEW METHOD FOR GENERATING AND SCREENING AN ANTIBODY BANK

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
WO1991007505A1 (en) * 1989-11-21 1991-05-30 Dynal A.S. Cloning method and kit
WO1994018219A1 (en) * 1993-02-02 1994-08-18 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
WO1997049809A1 (en) * 1996-06-24 1997-12-31 Rotkreuzstiftung Zentrallaboratorium Blutspendedienst Srk Polypeptides capable of forming antigen binding structures with specificity for the rhesus d antigens, the dna encoding them and the process for their preparation and use
WO2000056913A1 (en) * 1999-03-19 2000-09-28 Genetics Institute, Inc. Primers-attached vector elongation (pave): a 5'-directed cdna cloning strategy

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
ATE137269T1 (en) * 1990-01-26 1996-05-15 Abbott Lab IMPROVED METHOD FOR AMPLIFICATION OF NUCLIC ACID TARGET SEQUENCE USABLE FOR POLYMERASE AND LIGASED CHAIN REACTION
NO301082B1 (en) * 1995-07-20 1997-09-08 Statens Inst For Folkehelse Method of monitoring the result in Gap-LCR, as well as nucleic acid sequence for use in the method
AU714486B2 (en) * 1995-11-21 2000-01-06 Yale University Unimolecular segment amplification and detection
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
ATE335843T1 (en) * 1996-05-02 2006-09-15 Applera Corp QUANTIFICATION OF RNA TRANSCRIPTS USING GENOMIC DNA AS AN INTERNAL STANDARD OF THE AMPLIFICATION REACTION
US6610472B1 (en) * 2000-10-31 2003-08-26 Genetastix Corporation Assembly and screening of highly complex and fully human antibody repertoire in yeast

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4661450A (en) * 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides
WO1991007505A1 (en) * 1989-11-21 1991-05-30 Dynal A.S. Cloning method and kit
WO1994018219A1 (en) * 1993-02-02 1994-08-18 The Scripps Research Institute Methods for producing antibody libraries using universal or randomized immunoglobulin light chains
WO1997049809A1 (en) * 1996-06-24 1997-12-31 Rotkreuzstiftung Zentrallaboratorium Blutspendedienst Srk Polypeptides capable of forming antigen binding structures with specificity for the rhesus d antigens, the dna encoding them and the process for their preparation and use
WO2000056913A1 (en) * 1999-03-19 2000-09-28 Genetics Institute, Inc. Primers-attached vector elongation (pave): a 5'-directed cdna cloning strategy

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
C.F. BARBAS ET AL.: "Phage display vectors", PHAGE DISPLAY, A LABORATORY MANUAL, 2001, CSH, Laboratory Press, NY, pages 2.9 - 2.13, XP002227456 *
RADER C AND BARBAS CF III: "Phagemid cloning vector pComb3X", NCBI, 3 October 2000 (2000-10-03), Washington, DC, XP002198237 *

Also Published As

Publication number Publication date
AU2002226049A1 (en) 2002-06-18
US20030049731A1 (en) 2003-03-13
US20090011471A1 (en) 2009-01-08
WO2002046435A2 (en) 2002-06-13

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