WO2002048404A3 - Method and kit for enhancing the association rates of polynucleotides - Google Patents

Method and kit for enhancing the association rates of polynucleotides Download PDF

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Publication number
WO2002048404A3
WO2002048404A3 PCT/US2001/048592 US0148592W WO0248404A3 WO 2002048404 A3 WO2002048404 A3 WO 2002048404A3 US 0148592 W US0148592 W US 0148592W WO 0248404 A3 WO0248404 A3 WO 0248404A3
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WO
WIPO (PCT)
Prior art keywords
polynucleotides
kit
enhancing
association rates
complementary
Prior art date
Application number
PCT/US2001/048592
Other languages
French (fr)
Other versions
WO2002048404A2 (en
Inventor
Michael M Becker
Original Assignee
Gen Probe Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc filed Critical Gen Probe Inc
Priority to AU2002230901A priority Critical patent/AU2002230901B2/en
Priority to AU3090102A priority patent/AU3090102A/en
Priority to AT01991158T priority patent/ATE447043T1/en
Priority to JP2002550118A priority patent/JP2004537263A/en
Priority to CA002436857A priority patent/CA2436857A1/en
Priority to DE60140317T priority patent/DE60140317D1/en
Priority to EP01991158A priority patent/EP1377679B1/en
Publication of WO2002048404A2 publication Critical patent/WO2002048404A2/en
Publication of WO2002048404A3 publication Critical patent/WO2002048404A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

The present invention features a method and associated kit for increasing the association rate between polynucleotides having complementary, single-stranded regions, where the method includes providing to a test sample containing the complementary polynucleotides one or more synthetic, water soluble polycationic polymers.
PCT/US2001/048592 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides WO2002048404A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2002230901A AU2002230901B2 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides
AU3090102A AU3090102A (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides
AT01991158T ATE447043T1 (en) 2000-12-14 2001-12-07 METHOD AND KITS FOR IMPROVING POLYNUCLEOTIDE HYBRIDIZATION RATES
JP2002550118A JP2004537263A (en) 2000-12-14 2001-12-07 Methods and kits for enhancing the rate of polynucleotide association
CA002436857A CA2436857A1 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides
DE60140317T DE60140317D1 (en) 2000-12-14 2001-12-07 METHOD AND KITS FOR IMPROVING THE HYBRIDIZATION RATES OF POLYNUCLEOTIDES
EP01991158A EP1377679B1 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25553500P 2000-12-14 2000-12-14
US60/255,535 2000-12-14

Publications (2)

Publication Number Publication Date
WO2002048404A2 WO2002048404A2 (en) 2002-06-20
WO2002048404A3 true WO2002048404A3 (en) 2003-10-23

Family

ID=22968766

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/048592 WO2002048404A2 (en) 2000-12-14 2001-12-07 Method and kit for enhancing the association rates of polynucleotides

Country Status (8)

Country Link
US (2) US7374945B2 (en)
EP (1) EP1377679B1 (en)
JP (1) JP2004537263A (en)
AT (1) ATE447043T1 (en)
AU (2) AU3090102A (en)
CA (1) CA2436857A1 (en)
DE (1) DE60140317D1 (en)
WO (1) WO2002048404A2 (en)

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DE10237284A1 (en) * 2002-08-14 2004-03-04 Advalytix Ag nucleic acid detection
KR101137572B1 (en) * 2003-06-11 2012-05-30 이데라 파마슈티칼즈, 인코포레이티드 Stabilized immunomodulatory oligonucleotides
WO2006063065A2 (en) * 2004-12-08 2006-06-15 Gen-Probe Incorporated Detection of nucleic acids from multiple types of human papillomaviruses
JP5187847B2 (en) * 2005-05-06 2013-04-24 ジェン−プローブ・インコーポレーテッド Nucleic acid target capture method
US7488581B2 (en) * 2006-03-20 2009-02-10 Kabushiki Kaisha Toshiba Method for detecting a target nucleic acid sequence
CN107254513B (en) 2008-05-27 2021-04-20 安捷伦科技有限公司 Compositions and methods for detecting chromosomal aberrations using novel hybridization buffers
WO2010097655A1 (en) * 2009-02-26 2010-09-02 Dako Denmark A/S Compositions and methods for rna hybridization applications
WO2013046033A1 (en) 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP3604555A1 (en) 2011-10-14 2020-02-05 President and Fellows of Harvard College Sequencing by structure assembly
US11118226B2 (en) 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
CA2859761C (en) 2011-12-22 2023-06-20 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
MX2018005611A (en) 2015-11-03 2018-11-09 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix.
CA3210120C (en) 2016-04-25 2024-04-09 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
JP7239465B2 (en) * 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ Methods for preparing nucleic acid sequence libraries for detection by fluorescence in situ sequencing
CN109923216A (en) 2016-08-31 2019-06-21 哈佛学院董事及会员团体 By the detection combination of biomolecule to the method for the single test using fluorescent in situ sequencing
CA3019250A1 (en) 2017-04-14 2019-10-13 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
AU2018262533B2 (en) 2017-05-05 2024-01-18 Stryker European Operations Limited Surgical navigation system
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
EP3830302B1 (en) * 2018-08-01 2022-10-05 Gen-Probe Incorporated Compositions and methods for detecting nucleic acids of epstein-barr virus
CN109988842B (en) * 2019-04-19 2022-12-06 上海吉玛制药技术有限公司 Reagent group for in-situ detection of PDL1 by oligonucleotide labeled probe and application thereof
JP7428951B2 (en) * 2021-12-10 2024-02-07 日東紡績株式会社 Methods for detecting mutations in target base sequences of nucleic acids, methods for selectively inhibiting amplification of nucleic acids, and kits for carrying out these methods
WO2023106190A1 (en) * 2021-12-10 2023-06-15 日東紡績株式会社 Agent for raising melting temperature of double-stranded nucleic acid, and uses therefor

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Also Published As

Publication number Publication date
US7374945B2 (en) 2008-05-20
CA2436857A1 (en) 2002-06-20
WO2002048404A2 (en) 2002-06-20
AU3090102A (en) 2002-06-24
ATE447043T1 (en) 2009-11-15
EP1377679B1 (en) 2009-10-28
US20020164614A1 (en) 2002-11-07
US20040077014A1 (en) 2004-04-22
AU2002230901B2 (en) 2006-09-07
EP1377679A2 (en) 2004-01-07
JP2004537263A (en) 2004-12-16
DE60140317D1 (en) 2009-12-10

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