WO2002079495A3 - Genomics applications for modified oligonucleotides - Google Patents

Genomics applications for modified oligonucleotides

Info

Publication number
WO2002079495A3
WO2002079495A3 PCT/US2002/009691 US0209691W WO02079495A3 WO 2002079495 A3 WO2002079495 A3 WO 2002079495A3 US 0209691 W US0209691 W US 0209691W WO 02079495 A3 WO02079495 A3 WO 02079495A3
Authority
WO
WIPO (PCT)
Prior art keywords
modified oligonucleotides
nucleic acid
genomics applications
genomics
complexes
Prior art date
Application number
PCT/US2002/009691
Other languages
French (fr)
Other versions
WO2002079495A2 (en
Inventor
Eric B Kmiec
Howard B Gamper
Michael C Rice
Michael G Usher
Original Assignee
Univ Delaware
Eric B Kmiec
Howard B Gamper
Michael C Rice
Michael G Usher
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Delaware, Eric B Kmiec, Howard B Gamper, Michael C Rice, Michael G Usher filed Critical Univ Delaware
Priority to AU2002303176A priority Critical patent/AU2002303176A1/en
Priority to EP02731183A priority patent/EP1414840A4/en
Priority to PCT/US2002/031073 priority patent/WO2003027640A2/en
Priority to US10/260,150 priority patent/US7468244B2/en
Priority to AU2002341898A priority patent/AU2002341898A2/en
Priority to EP02776053A priority patent/EP1446502A4/en
Publication of WO2002079495A2 publication Critical patent/WO2002079495A2/en
Priority to US10/672,735 priority patent/US7229767B2/en
Publication of WO2002079495A3 publication Critical patent/WO2002079495A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Abstract

Methods for the production and use of stable complexes of duplex nucleic acid molecules and oligonucleotides are presented. These complexes can be used for the detection and purification of a known nucleic acid target as well as the manipulation of a defined nucleic acid target sequence.
PCT/US2002/009691 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides WO2002079495A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2002303176A AU2002303176A1 (en) 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides
EP02731183A EP1414840A4 (en) 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides
PCT/US2002/031073 WO2003027640A2 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation
US10/260,150 US7468244B2 (en) 2001-09-27 2002-09-27 Polymorphism detection and separation
AU2002341898A AU2002341898A2 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation
EP02776053A EP1446502A4 (en) 2001-09-28 2002-09-27 Polymorphism detection and separation
US10/672,735 US7229767B2 (en) 2001-03-27 2003-09-26 Genomics applications for modified OLIGO nucleotides

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US27914601P 2001-03-27 2001-03-27
US60/279,146 2001-03-27
US32582801P 2001-09-28 2001-09-28
US60/325,828 2001-09-28

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/260,150 Continuation-In-Part US7468244B2 (en) 2001-09-27 2002-09-27 Polymorphism detection and separation
US10/672,735 Continuation US7229767B2 (en) 2001-03-27 2003-09-26 Genomics applications for modified OLIGO nucleotides

Publications (2)

Publication Number Publication Date
WO2002079495A2 WO2002079495A2 (en) 2002-10-10
WO2002079495A3 true WO2002079495A3 (en) 2004-03-04

Family

ID=26959478

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/009691 WO2002079495A2 (en) 2001-03-27 2002-03-27 Genomics applications for modified oligonucleotides

Country Status (4)

Country Link
US (1) US7229767B2 (en)
EP (1) EP1414840A4 (en)
AU (1) AU2002303176A1 (en)
WO (1) WO2002079495A2 (en)

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EP1414840A4 (en) 2001-03-27 2005-04-13 Univ Delaware Genomics applications for modified oligonucleotides
US7468244B2 (en) 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
AU2002341898A2 (en) * 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
WO2007037676A1 (en) * 2005-09-29 2007-04-05 Keygene N.V. Method and means for targeted nucleotide exchange
US8951731B2 (en) * 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
AU2010279572B2 (en) * 2009-08-03 2017-02-02 Recombinetics, Inc. Methods and compositions for targeted gene modification
EP2880182B1 (en) 2012-08-02 2017-07-26 Qiagen GmbH Recombinase mediated targeted dna enrichment for next generation sequencing
CN111868245A (en) 2018-02-09 2020-10-30 豪夫迈·罗氏有限公司 Oligonucleotides for modulating TMEM106B expression
WO2024050304A1 (en) * 2022-08-29 2024-03-07 Illumina, Inc. Preparation and use of blocked substrates

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Also Published As

Publication number Publication date
US7229767B2 (en) 2007-06-12
US20050100921A1 (en) 2005-05-12
EP1414840A2 (en) 2004-05-06
EP1414840A4 (en) 2005-04-13
AU2002303176A1 (en) 2002-10-15
WO2002079495A2 (en) 2002-10-10

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