WO2004002627A8 - Method and apparatus for fluid dispersion - Google Patents

Method and apparatus for fluid dispersion

Info

Publication number
WO2004002627A8
WO2004002627A8 PCT/US2003/020542 US0320542W WO2004002627A8 WO 2004002627 A8 WO2004002627 A8 WO 2004002627A8 US 0320542 W US0320542 W US 0320542W WO 2004002627 A8 WO2004002627 A8 WO 2004002627A8
Authority
WO
WIPO (PCT)
Prior art keywords
fluid dispersion
fluid
focusing
readily
available
Prior art date
Application number
PCT/US2003/020542
Other languages
French (fr)
Other versions
WO2004002627A2 (en
WO2004002627A3 (en
WO2004002627B1 (en
Inventor
Howard A Stone
Shelly L Anna
Nathalie Bontoux
Darren Roy Link
David A Weitz
Irina Gitlin
Piotr Garstecki
Willow Diluzio
George M Whitesides
Eugenia Kumacheva
Original Assignee
Harvard College
Howard A Stone
Shelly L Anna
Nathalie Bontoux
Darren Roy Link
David A Weitz
Irina Gitlin
Piotr Garstecki
Willow Diluzio
George M Whitesides
Eugenia Kumacheva
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2004549845A priority Critical patent/JP2006507921A/en
Application filed by Harvard College, Howard A Stone, Shelly L Anna, Nathalie Bontoux, Darren Roy Link, David A Weitz, Irina Gitlin, Piotr Garstecki, Willow Diluzio, George M Whitesides, Eugenia Kumacheva filed Critical Harvard College
Priority to CN038204940A priority patent/CN1678397B/en
Priority to EP20030762228 priority patent/EP1515803A2/en
Priority to CA2491564A priority patent/CA2491564C/en
Priority to AU2003253751A priority patent/AU2003253751B2/en
Publication of WO2004002627A2 publication Critical patent/WO2004002627A2/en
Publication of WO2004002627A3 publication Critical patent/WO2004002627A3/en
Publication of WO2004002627B1 publication Critical patent/WO2004002627B1/en
Priority to US11/024,228 priority patent/US7708949B2/en
Publication of WO2004002627A8 publication Critical patent/WO2004002627A8/en
Priority to US12/726,223 priority patent/US8337778B2/en
Priority to US13/679,190 priority patent/US8986628B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/45—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads
    • B01F25/452—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces
    • B01F25/4521—Mixers in which the materials to be mixed are pressed together through orifices or interstitial spaces, e.g. between beads characterised by elements provided with orifices or interstitial spaces the components being pressed through orifices in elements, e.g. flat plates or cylinders, which obstruct the whole diameter of the tube
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0408—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing two or more liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05B—SPRAYING APPARATUS; ATOMISING APPARATUS; NOZZLES
    • B05B7/00—Spraying apparatus for discharge of liquids or other fluent materials from two or more sources, e.g. of liquid and air, of powder and gas
    • B05B7/02—Spray pistols; Apparatus for discharge
    • B05B7/04—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge
    • B05B7/0416—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid
    • B05B7/0441—Spray pistols; Apparatus for discharge with arrangements for mixing liquids or other fluent materials before discharge with arrangements for mixing one gas and one liquid with one inner conduit of liquid surrounded by an external conduit of gas upstream the mixing chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0418—Geometrical information
    • B01F2215/0431—Numerical size values, e.g. diameter of a hole or conduit, area, volume, length, width, or ratios thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0436—Operational information
    • B01F2215/045—Numerical flow-rate values
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
    • Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S516/00—Colloid systems and wetting agents; subcombinations thereof; processes of
    • Y10S516/924—Significant dispersive or manipulative operation or step in making or stabilizing colloid system
    • Y10S516/927—Significant dispersive or manipulative operation or step in making or stabilizing colloid system in situ formation of a colloid system making or stabilizing agent which chemical reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0324—With control of flow by a condition or characteristic of a fluid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0324—With control of flow by a condition or characteristic of a fluid
    • Y10T137/0329—Mixing of plural fluids of diverse characteristics or conditions
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/206—Flow affected by fluid contact, energy field or coanda effect [e.g., pure fluid device or system]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems
    • Y10T137/87265—Dividing into parallel flow paths with recombining
    • Y10T137/87338—Flow passage with bypass
    • Y10T137/87346—Including mixing feature
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T29/00—Metal working
    • Y10T29/49—Method of mechanical manufacture
    • Y10T29/49002—Electrical device making
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micromachines (AREA)

Abstract

A microfluidic method and device for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid is provided. The device can be fabricated simply from readily-available, inexpensive material using simple techniques.
PCT/US2003/020542 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion WO2004002627A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP2004549845A JP2006507921A (en) 2002-06-28 2003-06-03 Method and apparatus for fluid dispersion
CN038204940A CN1678397B (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
EP20030762228 EP1515803A2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
CA2491564A CA2491564C (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
AU2003253751A AU2003253751B2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion
US11/024,228 US7708949B2 (en) 2002-06-28 2004-12-28 Method and apparatus for fluid dispersion
US12/726,223 US8337778B2 (en) 2002-06-28 2010-03-17 Method and apparatus for fluid dispersion
US13/679,190 US8986628B2 (en) 2002-06-28 2012-11-16 Method and apparatus for fluid dispersion

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39219502P 2002-06-28 2002-06-28
US60/392,195 2002-06-28
US42404202P 2002-11-05 2002-11-05
US60/424,042 2002-11-05

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/024,228 Continuation US7708949B2 (en) 2002-06-28 2004-12-28 Method and apparatus for fluid dispersion

Publications (4)

Publication Number Publication Date
WO2004002627A2 WO2004002627A2 (en) 2004-01-08
WO2004002627A3 WO2004002627A3 (en) 2004-04-01
WO2004002627B1 WO2004002627B1 (en) 2004-06-17
WO2004002627A8 true WO2004002627A8 (en) 2005-03-17

Family

ID=30003231

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/020542 WO2004002627A2 (en) 2002-06-28 2003-06-30 Method and apparatus for fluid dispersion

Country Status (7)

Country Link
US (3) US7708949B2 (en)
EP (2) EP1515803A2 (en)
JP (2) JP2006507921A (en)
CN (2) CN1678397B (en)
AU (1) AU2003253751B2 (en)
CA (1) CA2491564C (en)
WO (1) WO2004002627A2 (en)

Families Citing this family (418)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
EP3616781A1 (en) 2003-04-10 2020-03-04 President and Fellows of Harvard College Formation and control of fluidic species
EP1658133A1 (en) 2003-08-27 2006-05-24 President And Fellows Of Harvard College Electronic control of fluidic species
US20050221339A1 (en) * 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US8696952B2 (en) * 2004-04-23 2014-04-15 Eugenia Kumacheva Method of producing polymeric particles with selected size, shape, morphology and composition
NL1026261C2 (en) 2004-05-25 2005-11-28 Nanomi B V Spraying device with a nozzle plate provided with structures for promoting self-breakup, a nozzle plate, and methods for manufacturing and using such a nozzle plate.
GB0502398D0 (en) * 2005-02-04 2005-03-16 Q Chip Ltd Device and method for producing spherical segmented flow
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
EP1839760A1 (en) * 2005-01-17 2007-10-03 Universidad de Sevilla Method and device for the micromixing of fluids using a reflux cell
WO2006078841A1 (en) * 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
EP1859330B1 (en) 2005-01-28 2012-07-04 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
JP2008535644A (en) 2005-03-04 2008-09-04 プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ Method and apparatus for the formation of multiple emulsions
JP2006289250A (en) * 2005-04-08 2006-10-26 Kao Corp Micro mixer and fluid mixing method using the same
US20060280029A1 (en) * 2005-06-13 2006-12-14 President And Fellows Of Harvard College Microfluidic mixer
EP2660482B1 (en) * 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
EP1946186B1 (en) * 2005-10-25 2017-08-09 Massachusetts Institute of Technology Microstructure synthesis by flow lithography and polymerization
US7709544B2 (en) 2005-10-25 2010-05-04 Massachusetts Institute Of Technology Microstructure synthesis by flow lithography and polymerization
GB2433448B (en) 2005-12-20 2011-03-02 Q Chip Ltd Method for the control of chemical processes
US20100137163A1 (en) * 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
JP4713397B2 (en) * 2006-01-18 2011-06-29 株式会社リコー Microchannel structure and microdroplet generation system
JP2009524825A (en) 2006-01-27 2009-07-02 プレジデント アンド フェロウズ オブ ハーバード カレッジ Fluid droplet coalescence
WO2007102785A1 (en) * 2006-03-09 2007-09-13 Agency For Science, Technology And Research Apparatus for performing a reaction in a droplet and method of using the same
WO2008105773A2 (en) * 2006-03-31 2008-09-04 Massachusetts Institute Of Technology System for targeted delivery of therapeutic agents
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8980198B2 (en) * 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7901947B2 (en) 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
WO2007133590A2 (en) * 2006-05-08 2007-11-22 Auburn University Systems for and methods of characterizing reactions
WO2009111769A2 (en) 2008-03-07 2009-09-11 Advanced Liquid Logic, Inc. Reagent and sample preparation and loading on a fluidic device
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
US20080003142A1 (en) 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
KR100848559B1 (en) * 2006-06-29 2008-07-25 엘지디스플레이 주식회사 FABRICATING METHOD OF SOFT MOLD AND pattern forming METHOD USING THEREOF
US7892434B2 (en) * 2006-08-02 2011-02-22 The Regents Of The University Of California Microfluidic production of monodispersed submicron emulsion through filtration and sorting of satellite drops
US9012390B2 (en) 2006-08-07 2015-04-21 Raindance Technologies, Inc. Fluorocarbon emulsion stabilizing surfactants
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
JP5560039B2 (en) 2006-10-05 2014-07-23 マサチューセッツ インスティテュート オブ テクノロジー Multi-functional coded particles for high-throughput analysis
WO2008063135A1 (en) 2006-11-24 2008-05-29 Agency For Science, Technology And Research Apparatus for processing a sample in a liquid droplet and method of using the same
US9874501B2 (en) 2006-11-24 2018-01-23 Curiox Biosystems Pte Ltd. Use of chemically patterned substrate for liquid handling, chemical and biological reactions
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
EP2573562A3 (en) 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US8691164B2 (en) * 2007-04-20 2014-04-08 Celula, Inc. Cell sorting system and methods
CA2689427C (en) 2007-06-05 2015-12-29 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009005680A1 (en) * 2007-06-29 2009-01-08 President And Fellows Of Harvard College Methods and apparatus for manipulation of fluidic species
GB0712863D0 (en) 2007-07-03 2007-08-08 Eastman Kodak Co Monodisperse droplet generation
GB0712861D0 (en) * 2007-07-03 2007-08-08 Eastman Kodak Co Continuous ink jet printing of encapsulated droplets
US20090068170A1 (en) * 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
US8883291B2 (en) * 2007-08-07 2014-11-11 President And Fellows Of Harvard College Metal oxide coating on surfaces
CN104307581B (en) * 2007-08-09 2017-04-12 诺思可有限公司 Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
JP5023902B2 (en) * 2007-09-06 2012-09-12 株式会社日立プラントテクノロジー Emulsifying device
US8685323B2 (en) * 2007-09-19 2014-04-01 Massachusetts Institute Of Technology Virus/nanowire encapsulation within polymer microgels for 2D and 3D devices for energy and electronics
WO2009037680A2 (en) 2007-09-20 2009-03-26 Jean-Louis Viovy Encapsulation microfluidic device
WO2009045050A2 (en) * 2007-10-05 2009-04-09 Seoul National University Industry Foundation Fluidic channel system and method for fabricating fine structure
US20090098168A1 (en) * 2007-10-08 2009-04-16 The Regents Of The University Of California Multiple-layer microbubble liposphere drug delivery vehicle and system
US10725020B2 (en) 2007-11-14 2020-07-28 Curiox Biosystems Pte Ltd. High throughput miniaturized assay system and methods
WO2013114217A1 (en) 2012-02-05 2013-08-08 Curiox Biosystems Pte Ltd. Array plates and methods for making and using same
JP5738597B2 (en) 2007-12-21 2015-06-24 プレジデント アンド フェローズ オブ ハーバード カレッジ Systems and methods for nucleic acid sequencing
CN101945767B (en) 2007-12-23 2013-10-30 先进液体逻辑公司 Droplet actuator configurations and methods of conducting droplet operations
JP2011515236A (en) * 2008-03-28 2011-05-19 プレジデント アンド フェローズ オブ ハーバード カレッジ Surface comprising microfluidic channels with controlled wetting properties
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
EP3002489B1 (en) 2008-05-16 2017-09-20 President and Fellows of Harvard College Valves and other flow control in fluidic systems including microfluidic systems
JP4572973B2 (en) * 2008-06-16 2010-11-04 ソニー株式会社 Microchip and flow-feeding method in microchip
EP2307560A2 (en) * 2008-06-27 2011-04-13 Massachusetts Institute of Technology Microfluidic droplets for metabolic engineering and other applications
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
JP2010038866A (en) * 2008-08-08 2010-02-18 Sony Corp Microchip, particulate dispensing device, and feed flow method
DE102008039117B3 (en) * 2008-08-21 2010-05-20 Institut für Bioprozess- und Analysenmesstechnik e.V. Arrangement and method for generating, manipulating and analyzing compartments
WO2010033200A2 (en) 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
WO2010104604A1 (en) 2009-03-13 2010-09-16 President And Fellows Of Harvard College Method for the controlled creation of emulsions, including multiple emulsions
KR101793744B1 (en) * 2009-03-13 2017-11-03 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Scale-up of flow-focusing microfluidic devices
EP3415235A1 (en) 2009-03-23 2018-12-19 Raindance Technologies Inc. Manipulation of microfluidic droplets
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
JP5766178B2 (en) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ Slipchip apparatus and method
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
WO2010110843A1 (en) 2009-03-25 2010-09-30 Eastman Kodak Company Droplet generator
US8689981B2 (en) 2009-04-10 2014-04-08 President And Fellows Of Harvard College Manipulation of particles in channels
SG177369A1 (en) 2009-06-26 2012-02-28 Harvard College Fluid injection
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
KR20120089661A (en) * 2009-09-02 2012-08-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Multiple emulsions created using jetting and other techniques
EP2940153B1 (en) 2009-09-02 2020-05-13 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US8746285B2 (en) * 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US10520500B2 (en) 2009-10-09 2019-12-31 Abdeslam El Harrak Labelled silica-based nanomaterial with enhanced properties and uses thereof
TWI421339B (en) * 2009-10-16 2014-01-01 Academia Sinica Method of fabricating three dimensional scaffolds and device thereof
US8513014B2 (en) * 2009-10-20 2013-08-20 Academia Sinica Method for fabricating foam scaffolds to culture cells
EP3842150A1 (en) 2009-10-27 2021-06-30 President and Fellows of Harvard College Droplet creation techniques
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
CN107028886A (en) 2009-11-04 2017-08-11 不列颠哥伦比亚大学 Lipid particle and related methods containing nucleic acid
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
ES2574055T3 (en) 2009-11-25 2016-06-14 Gen9, Inc. Methods and apparatus for error reduction in chip-based DNA
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011079176A2 (en) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
WO2011100604A2 (en) 2010-02-12 2011-08-18 Raindance Technologies, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US8399198B2 (en) 2010-03-02 2013-03-19 Bio-Rad Laboratories, Inc. Assays with droplets transformed into capsules
US8716467B2 (en) 2010-03-03 2014-05-06 Gen9, Inc. Methods and devices for nucleic acid synthesis
JP2013525087A (en) 2010-03-17 2013-06-20 プレジデント アンド フェロウズ オブ ハーバード カレッジ Melt emulsification
EP2550528B1 (en) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
EP2550351A4 (en) 2010-03-25 2014-07-09 Quantalife Inc Detection system for droplet-based assays
USD673286S1 (en) 2010-04-29 2012-12-25 Sony Corporation Micro flow channel chip
USD869308S1 (en) 2010-04-29 2019-12-10 Sony Corporation Micro flow channel chip
USD673287S1 (en) 2010-11-24 2012-12-25 Sony Corporation Micro flow channel chip
US9476101B2 (en) 2010-06-07 2016-10-25 Firefly Bioworks, Inc. Scanning multifunctional particles
US9499813B2 (en) 2010-06-10 2016-11-22 President And Fellows Of Harvard College Systems and methods for amplification and phage display
JP2012024313A (en) * 2010-07-23 2012-02-09 Nitto Denko Corp Device for forming droplets, and method for forming droplets
US9878328B2 (en) 2010-07-23 2018-01-30 Curiox Biosystems Pte Ltd. Apparatus and method for multiple reactions in small volumes
EP2608878A4 (en) 2010-08-23 2017-11-15 President and Fellows of Harvard College Acoustic waves in microfluidics
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
CA3140602C (en) 2010-11-01 2023-10-03 Bio-Rad Laboratories, Inc. System for forming emulsions
EP3360963B1 (en) 2010-11-12 2019-11-06 Gen9, Inc. Methods and devices for nucleic acids synthesis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
EP2654939A2 (en) 2010-12-21 2013-10-30 President and Fellows of Harvard College Spray drying techniques
CN103347612B (en) * 2011-02-07 2016-03-16 哈佛学院院长等 The system and method for division drop
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
US9364803B2 (en) 2011-02-11 2016-06-14 Raindance Technologies, Inc. Methods for forming mixed droplets
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
AU2012231098B2 (en) 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
EP2691540B1 (en) 2011-03-31 2016-01-20 GnuBIO, Inc. Managing variation in spectroscopic intensity measurements through the use of a reference component
ES2656557T3 (en) 2011-03-31 2018-02-27 Dana-Farber Cancer Institute, Inc. Method to enrich single-stranded mutant sequences from a mixture of natural and mutant sequences
EP2702175B1 (en) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
WO2012154745A2 (en) 2011-05-09 2012-11-15 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
KR20140034242A (en) 2011-05-23 2014-03-19 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Control of emulsions, including multiple emulsions
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
BR112014000257A2 (en) 2011-07-06 2017-03-01 Advanced Liquid Logic Inc reagent storage in a drop actuator
EP3120923A3 (en) 2011-07-06 2017-03-01 President and Fellows of Harvard College Article comprising a particle having a shell and a fluid
WO2013009927A2 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based assays
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
AU2012300401B2 (en) 2011-08-26 2018-02-08 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
EP2750787A2 (en) 2011-08-30 2014-07-09 President and Fellows of Harvard College Systems and methods for shell encapsulation
CN106423314B (en) 2011-09-28 2021-03-02 哈佛学院院长等 Systems and methods for droplet generation and/or fluid manipulation
US20140328759A1 (en) 2011-10-25 2014-11-06 The University Of British Columbia Limit size lipid nanoparticles and related methods
CN102500489A (en) * 2011-11-06 2012-06-20 中国科学技术大学 Spray gun spray nozzle capable of realizing minuteness atomization
US10731199B2 (en) 2011-11-21 2020-08-04 Advanced Liquid Logic, Inc. Glucose-6-phosphate dehydrogenase assays
US8771611B2 (en) 2011-11-28 2014-07-08 Auburn University System and methods of log-scale concentration gradients
JP2015513451A (en) 2012-02-08 2015-05-14 プレジデント アンド フェローズ オブ ハーバード カレッジ Droplet formation using fluid splitting
EP3495817A1 (en) 2012-02-10 2019-06-12 Raindance Technologies, Inc. Molecular diagnostic screening assay
EP2817418B1 (en) 2012-02-24 2017-10-11 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
LT3305918T (en) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Methods for epigenetic sequencing
JP6115930B2 (en) * 2012-03-12 2017-04-19 国立研究開発法人産業技術総合研究所 Multi-stage split channel mixer
US10080997B2 (en) 2012-03-16 2018-09-25 Versitech Limited System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US9782733B2 (en) * 2012-03-22 2017-10-10 Universiteit Twente Apparatus and method for mass producing a monodisperse microbubble agent
US8936354B2 (en) 2012-03-28 2015-01-20 Eastman Kodak Company Digital drop patterning device and method
US8936353B2 (en) 2012-03-28 2015-01-20 Eastman Kodak Company Digital drop patterning device and method
US8602535B2 (en) 2012-03-28 2013-12-10 Eastman Kodak Company Digital drop patterning device and method
US8939551B2 (en) 2012-03-28 2015-01-27 Eastman Kodak Company Digital drop patterning device and method
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
WO2013159116A1 (en) 2012-04-20 2013-10-24 University Of Chicago Fluidic devices for biospecimen preservation
WO2013159117A1 (en) 2012-04-20 2013-10-24 SlipChip, LLC Fluidic devices and systems for sample preparation or autonomous analysis
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
WO2013163246A2 (en) 2012-04-25 2013-10-31 President And Fellows Of Harvard College Polymerization reactions within microfluidic devices
WO2013165748A1 (en) 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
CN104685116A (en) 2012-06-25 2015-06-03 Gen9股份有限公司 Methods for nucleic acid assembly and high throughput sequencing
WO2014004908A1 (en) 2012-06-27 2014-01-03 Advanced Liquid Logic Inc. Techniques and droplet actuator designs for reducing bubble formation
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US9328376B2 (en) * 2012-09-05 2016-05-03 Bio-Rad Laboratories, Inc. Systems and methods for stabilizing droplets
EP2895591A4 (en) 2012-09-12 2016-10-12 Gnubio Inc Integrated microfluidic system, method and kit for performing assays
WO2014047236A2 (en) 2012-09-21 2014-03-27 President And Fellows Of Harvard College Systems and methods for spray drying in microfluidic and other systems
WO2014085801A1 (en) 2012-11-30 2014-06-05 The Broad Institute, Inc. Cryo-treatment in a microfluidic device
EP3567116A1 (en) 2012-12-14 2019-11-13 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR102190198B1 (en) 2013-02-08 2020-12-14 10엑스 제노믹스, 인크. Polynucleotide barcode generation
WO2014138154A1 (en) * 2013-03-06 2014-09-12 President And Fellows Of Harvard College Devices and methods for forming relatively monodisperse droplets
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
EP2971013B1 (en) 2013-03-15 2020-08-19 The University Of British Columbia Lipid nanoparticles for transfection and related methods
GB201306444D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
GB201306445D0 (en) 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
EP2986762B1 (en) 2013-04-19 2019-11-06 Bio-Rad Laboratories, Inc. Digital analyte analysis
CN103285947A (en) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 Droplet micro-fluidic chip and operation method thereof
CN103285946A (en) * 2013-05-27 2013-09-11 苏州扬清芯片科技有限公司 Biochip and control method thereof
KR20140144408A (en) * 2013-06-11 2014-12-19 삼성전기주식회사 Droplet forming device and method for forming droplet using the same
WO2014201196A2 (en) 2013-06-14 2014-12-18 President And Fellows Of Harvard College Coalescence of droplets
US9557318B2 (en) 2013-07-09 2017-01-31 Curiox Biosystems Pte Ltd. Array plates for washing samples
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9233859B2 (en) 2013-09-30 2016-01-12 Uchicago Argonne, Llc. Microfluidic process monitor for industrial solvent extraction system
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US20160279068A1 (en) 2013-11-08 2016-09-29 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US10801070B2 (en) 2013-11-25 2020-10-13 The Broad Institute, Inc. Compositions and methods for diagnosing, evaluating and treating cancer
US11725237B2 (en) 2013-12-05 2023-08-15 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015095811A2 (en) 2013-12-20 2015-06-25 The Board Institute Inc. Combination therapy with neoantigen vaccine
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
JP2017508457A (en) 2014-02-27 2017-03-30 ザ・ブロード・インスティテュート・インコーポレイテッド T cell balance gene expression, composition and method of use thereof
EP3117897A4 (en) * 2014-03-11 2017-10-18 Toppan Printing Co., Ltd. Droplet producing device, droplet producing method, liposome producing method, fixture, and droplet producing kit
DE202015009494U1 (en) 2014-04-10 2018-02-08 10X Genomics, Inc. Fluidic devices and systems for encapsulating and partitioning reagents, and their applications
WO2015160919A1 (en) 2014-04-16 2015-10-22 President And Fellows Of Harvard College Systems and methods for producing droplet emulsions with relatively thin shells
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
DK3299469T3 (en) 2014-04-21 2020-03-30 Harvard College SYSTEMS AND PROCEDURES FOR BARCELING MARKING NUCLEIC ACID
CA2948979A1 (en) 2014-05-14 2015-11-19 University Of Limerick Microfluidic devices that include channels that are slidable relative to each other and methods of use thereof
CN106536709A (en) 2014-06-16 2017-03-22 基纽拜奥股份有限公司 Size alternating injection into drops to facilitate sorting
MX2016016902A (en) 2014-06-26 2017-03-27 10X Genomics Inc Methods of analyzing nucleic acids from individual cells or cell populations.
US10258987B2 (en) 2014-06-26 2019-04-16 President And Fellows Of Harvard College Fluid infection using acoustic waves
KR20170023979A (en) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. Processes and systems for nucleic acid sequence assembly
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
EP3177405B1 (en) 2014-08-06 2020-05-06 S.C. Johnson & Son, Inc. Spray inserts
EP4105337A1 (en) 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
ES2727656T3 (en) 2014-09-15 2019-10-17 Abvitro Llc High performance sequencing of nucleotide banks
EP3209419A4 (en) 2014-10-22 2018-10-03 The Regents of The University of California High definition microdroplet printer
CN107002128A (en) 2014-10-29 2017-08-01 10X 基因组学有限公司 The method and composition being sequenced for target nucleic acid
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
WO2016077750A1 (en) 2014-11-14 2016-05-19 Athena Diagnostics, Inc. Methods to detect a silent carrier genotype
JP2017537901A (en) 2014-11-24 2017-12-21 ザ プロクター アンド ギャンブル カンパニー Compositions containing active substances encapsulated in droplets and other compartments
EP3234193B1 (en) 2014-12-19 2020-07-15 Massachusetts Institute of Technology Molecular biomarkers for cancer immunotherapy
US10993997B2 (en) 2014-12-19 2021-05-04 The Broad Institute, Inc. Methods for profiling the t cell repertoire
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
SG11201705425SA (en) 2015-01-13 2017-08-30 10X Genomics Inc Systems and methods for visualizing structural variation and phasing information
NL2014178B1 (en) * 2015-01-22 2017-01-05 Tide Microfluidics B V System and method for controlled manufacturing of mono-disperse microbubbles.
EP3247675A4 (en) 2015-01-23 2018-07-04 Neofluidics LLC A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors
US20160238506A1 (en) * 2015-02-02 2016-08-18 Derek Oberreit Ice nucleii counter technology
CA2974306A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US20160358722A1 (en) * 2015-02-05 2016-12-08 Ramasamy Lakshmanan Intelligent wireless and wired control of devices
MX2017010142A (en) 2015-02-09 2017-12-11 10X Genomics Inc Systems and methods for determining structural variation and phasing using variant call data.
EP3256850A4 (en) 2015-02-09 2018-10-10 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same
AU2016222719B2 (en) 2015-02-24 2022-03-31 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
EP3262193A2 (en) 2015-02-26 2018-01-03 The Broad Institute Inc. T cell balance gene expression, compositions of matters and methods of use thereof
US11873483B2 (en) 2015-03-11 2024-01-16 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
CN107614096A (en) 2015-03-13 2018-01-19 哈佛学院院长及董事 Use amplification assay cell
US11746367B2 (en) 2015-04-17 2023-09-05 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
IL302102A (en) 2015-05-20 2023-06-01 Dana Farber Cancer Inst Inc Shared neoantigens
WO2016189383A1 (en) * 2015-05-22 2016-12-01 The Hong Kong University Of Science And Technology Droplet generator based on high aspect ratio induced droplet self-breakup
US10545139B2 (en) 2015-06-16 2020-01-28 Curiox Biosystems Pte Ltd. Methods and devices for performing biological assays using magnetic components
WO2016205728A1 (en) 2015-06-17 2016-12-22 Massachusetts Institute Of Technology Crispr mediated recording of cellular events
EP3314046B1 (en) 2015-06-25 2019-04-24 Hooke Bio Limited Mechanical device for generating combinatorial library
US11690309B2 (en) 2015-07-23 2023-07-04 Zito Jr Arthur J Responsive dispersion from compartment in aqueous solution
EP3341508A4 (en) 2015-08-25 2019-05-15 Bio-Rad Laboratories, Inc. Digital immunoassay
AU2016311341B2 (en) 2015-08-27 2021-01-28 President And Fellows Of Harvard College Acoustic wave sorting
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
KR20180085717A (en) 2015-09-24 2018-07-27 에이비비트로, 엘엘씨 Affinity-oligonucleotide conjugates and their use
WO2018057051A1 (en) 2016-09-24 2018-03-29 Abvitro Llc Affinity-oligonucleotide conjugates and uses thereof
WO2017053903A1 (en) 2015-09-24 2017-03-30 Abvitro Llc Single amplicon activated exclusion pcr
CN113774495A (en) 2015-09-25 2021-12-10 阿布维特罗有限责任公司 High throughput method for T cell receptor targeted identification of naturally paired T cell receptor sequences
CN108289797B (en) 2015-10-13 2022-01-28 哈佛学院院长及董事 Systems and methods for making and using gel microspheres
EP3370868B1 (en) 2015-10-27 2020-12-09 Berkeley Lights, Inc. Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
CN105435869B (en) * 2015-11-06 2017-05-10 常州工学院 Apparatus and method for micro-droplet split in microchannel
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
CN108291223B (en) 2015-11-20 2022-06-07 生物辐射实验室股份有限公司 Sparse identification space in droplet sequencing
DK3882357T3 (en) 2015-12-04 2022-08-29 10X Genomics Inc Methods and compositions for the analysis of nucleic acids
EP3263715B1 (en) 2016-06-28 2020-01-08 Hifibio Method for transcriptome analysis of single cells
US20190144936A1 (en) 2016-01-15 2019-05-16 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
EP3411710A1 (en) 2016-02-05 2018-12-12 The Broad Institute Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations
JP6735348B2 (en) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド Systems, methods and media for de novo assembly of whole genome sequence data
US20190144942A1 (en) 2016-02-22 2019-05-16 Massachusetts Institute Of Technology Methods for identifying and modulating immune phenotypes
US11427861B2 (en) 2016-03-17 2022-08-30 Massachusetts Institute Of Technology Methods for identifying and modulating co-occurant cellular phenotypes
WO2017168332A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex target capture
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
CA3020913C (en) 2016-04-15 2024-01-02 President And Fellows Of Harvard College Systems and methods for the collection of droplets and/or other entities
CN105712319B (en) * 2016-04-29 2018-01-23 清华大学 The preparation facilities of macroscopical aggregation of micro Nano material
CN109475709B (en) 2016-05-03 2022-12-27 精呼吸股份有限公司 Method of generating and delivering droplets to the pulmonary system using a droplet delivery device
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
CN107414080B (en) * 2016-05-23 2024-04-16 中国科学院理化技术研究所 Liquid metal 3D prints shower nozzle device and is equipped with device's 3D printer
CN109689887A (en) 2016-07-08 2019-04-26 加州理工学院 For carrying out the method and apparatus for flowing through capture of analytes in low concentration
WO2018009766A1 (en) 2016-07-08 2018-01-11 President And Fellows Of Harvard College Formation of colloids or gels within droplets
WO2018031691A1 (en) 2016-08-10 2018-02-15 The Regents Of The University Of California Combined multiple-displacement amplification and pcr in an emulsion microdroplet
US10654040B2 (en) 2016-08-18 2020-05-19 Northeastern University Platform for liquid droplet formation and isolation
EP3551769A4 (en) 2016-12-09 2020-10-28 Boreal Genomics, Inc. Linked ligation
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140391A1 (en) 2017-01-24 2018-08-02 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
EP4029939B1 (en) 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US20200115753A1 (en) 2017-03-17 2020-04-16 Massachusetts Institute Of Technology Methods for identifying and modulating co-occurant cellular phenotypes
EP3375889B1 (en) 2017-03-17 2019-12-11 HiFiBiO SAS Single cell analysis
WO2018185554A1 (en) 2017-04-05 2018-10-11 Curiox Biosystems Pte Ltd. Methods, devices, and apparatus for washing samples on array plates
US20210293783A1 (en) 2017-04-18 2021-09-23 The General Hospital Corporation Compositions for detecting secretion and methods of use
EP3615220A4 (en) 2017-04-28 2020-12-30 Neofluidics, LLC Fluidic devices with reaction wells and uses thereof
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US11920192B2 (en) 2017-05-15 2024-03-05 Lightcast Discovery Ltd Single nucleotide detection method and associated probes
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
CN110945139B (en) 2017-05-18 2023-09-05 10X基因组学有限公司 Method and system for sorting droplets and beads
CN110870018A (en) 2017-05-19 2020-03-06 10X基因组学有限公司 System and method for analyzing a data set
US11529476B2 (en) 2017-05-19 2022-12-20 Pneuma Respiratory, Inc. Dry powder delivery device and methods of use
WO2018217831A1 (en) 2017-05-22 2018-11-29 Arizona Board Of Regents On Behalf Of Arizona State University Metal electrode based 3d printed device for tuning microfluidic droplet generation frequency and synchronizing phase for serial femtosecond crystallography
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (en) 2017-05-26 2022-11-25 10X基因组学有限公司 Single cell analysis of transposase accessible chromatin
US11745181B2 (en) 2017-08-09 2023-09-05 Unchained Labs Devices and methods for bioassay
US10549279B2 (en) 2017-08-22 2020-02-04 10X Genomics, Inc. Devices having a plurality of droplet formation regions
WO2019051335A1 (en) 2017-09-07 2019-03-14 Juno Therapeutics, Inc. Methods of identifying cellular attributes related to outcomes associated with cell therapy
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
JP2020536614A (en) 2017-10-04 2020-12-17 ニューマ・リスパイラトリー・インコーポレイテッド In-line droplet delivery device and usage that is electrically operated by breathing
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019079461A1 (en) 2017-10-17 2019-04-25 Pneuma Respiratory, Inc. Nasal drug delivery apparatus and methods of use
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
WO2019079301A2 (en) 2017-10-18 2019-04-25 Group K Diagnostics, Inc. Single-layer microfluidic device and methods of manufacture and use thereof
WO2019084055A1 (en) 2017-10-23 2019-05-02 Massachusetts Institute Of Technology Calling genetic variation from single-cell transcriptomes
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
EP3700672B1 (en) 2017-10-27 2022-12-28 10X Genomics, Inc. Methods for sample preparation and analysis
JP2021502178A (en) 2017-11-08 2021-01-28 ニューマ・リスパイラトリー・インコーポレイテッド In-line droplet delivery device with a small volume ampoule and electrically actuated by breathing and how to use
US11305279B2 (en) 2017-11-10 2022-04-19 Neofluidics, Llc Integrated fluidic circuit and device for droplet manipulation and methods thereof
EP3625361A1 (en) 2017-11-15 2020-03-25 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN107941659B (en) * 2017-11-20 2020-05-19 武汉科技大学 Seepage velocity measuring device in fracture water freezing process
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
US11173487B2 (en) 2017-12-19 2021-11-16 Arizona Board Of Regents On Behalf Of Arizona State University Deterministic ratchet for sub-micrometer bioparticle separation
CN111787866A (en) 2017-12-28 2020-10-16 爱惜康有限责任公司 Surgical instrument including control system using input from strain gauge circuitry
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN112423893A (en) * 2018-06-14 2021-02-26 明尼苏达大学董事会 Counter-current mixer and atomizer
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112996605A (en) * 2018-10-01 2021-06-18 精呼吸股份有限公司 Delivery of low surface tension compositions to the pulmonary system via an electronic breath-actuated droplet delivery device
US20220411783A1 (en) 2018-10-12 2022-12-29 The Broad Institute, Inc. Method for extracting nuclei or whole cells from formalin-fixed paraffin-embedded tissues
GB201817321D0 (en) 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
USD879999S1 (en) 2018-11-02 2020-03-31 Group K Diagnostics, Inc. Microfluidic device
SG11202105083XA (en) 2018-11-14 2021-06-29 Broad Inst Inc Crispr system based droplet diagnostic systems and methods
SG11202105082SA (en) 2018-11-14 2021-06-29 Harvard College Multiplexing highly evolving viral variants with sherlock detection method
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123657A2 (en) 2018-12-11 2020-06-18 10X Genomics, Inc. Methods and devices for detecting and sorting droplets or particles
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
US20220062394A1 (en) 2018-12-17 2022-03-03 The Broad Institute, Inc. Methods for identifying neoantigens
EP3670667A1 (en) 2018-12-19 2020-06-24 Paris Sciences et Lettres - Quartier Latin Identification of cognate pairs of ligands and receptors
WO2020139844A1 (en) 2018-12-24 2020-07-02 10X Genomics, Inc. Devices, systems, and methods for controlling liquid flow
US11473136B2 (en) 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
WO2020160044A1 (en) 2019-01-28 2020-08-06 The Broad Institute, Inc. In-situ spatial transcriptomics
WO2020168013A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
EP3698871A1 (en) 2019-02-19 2020-08-26 Gottfried Wilhelm Leibniz Universität Hannover Laser based sorting of droplets in microfluidic streams
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
WO2020176449A1 (en) 2019-02-26 2020-09-03 President And Fellows Of Harvard College Systems and methods for high throughput selection
WO2020176882A1 (en) 2019-02-28 2020-09-03 10X Genomics, Inc. Devices, systems, and methods for increasing droplet formation efficiency
US11920183B2 (en) 2019-03-11 2024-03-05 10X Genomics, Inc. Systems and methods for processing optically tagged beads
US11318487B2 (en) * 2019-05-14 2022-05-03 Arizona Board Of Regents On Behalf Of Arizona State University Co-flow injection for serial crystallography
US11624718B2 (en) 2019-05-14 2023-04-11 Arizona Board Of Regents On Behalf Of Arizona State University Single piece droplet generation and injection device for serial crystallography
AU2020308104A1 (en) * 2019-06-27 2022-02-03 Pneuma Respiratory, Inc. Delivery of small droplets to the respiratory system via electronic breath actuated droplet delivery device
EP3763439A1 (en) * 2019-07-12 2021-01-13 Curiosity Diagnostics sp. z o.o Microfluidic chip and valve, production process and uses
CN110404701A (en) * 2019-07-16 2019-11-05 清华大学 A kind of control method and device of jet crushing
US11701658B2 (en) 2019-08-09 2023-07-18 President And Fellows Of Harvard College Systems and methods for microfluidic particle selection, encapsulation, and injection using surface acoustic waves
US11919002B2 (en) 2019-08-20 2024-03-05 10X Genomics, Inc. Devices and methods for generating and recovering droplets
GB201914537D0 (en) 2019-10-08 2019-11-20 Univ Southampton Transcript analysis
WO2021072306A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN110787846B (en) * 2019-11-05 2021-04-16 华中科技大学 One-step double-layer micro-droplet generation device and method
AU2021209907A1 (en) 2020-01-24 2022-07-07 Slingshot Biosciences, Inc. Compositions and methods for cell-like calibration particles
US20230158502A1 (en) 2020-04-17 2023-05-25 Sphere Fluidics Limited Droplet spacing
WO2021226036A1 (en) 2020-05-04 2021-11-11 Slingshot Biosciences, Inc. Compositions and methods for passive optical barcoding for multiplexed assays
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11594340B2 (en) 2020-05-13 2023-02-28 Battelle Savannah River Alliance, Llc Manufacture of particulate reference materials
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
CN115997125A (en) 2020-06-24 2023-04-21 高诚生物医药(香港)有限公司 Method for identifying cognate pairs of ligand and receptor
EP3950772A1 (en) 2020-08-05 2022-02-09 Emulseo SAS Novel fluorosurfactants and uses thereof in microfluidics
CN116171200A (en) 2020-09-02 2023-05-26 10X基因组学有限公司 Apparatus, system and method for high throughput drop formation
CN116367913A (en) 2020-09-02 2023-06-30 10X基因组学有限公司 Flow focusing apparatus, systems, and methods for high throughput drop formation
US11485632B2 (en) 2020-10-09 2022-11-01 Arizona Board Of Regents On Behalf Of Arizona State University Modular 3-D printed devices for sample delivery and method
WO2022146770A1 (en) 2020-12-28 2022-07-07 Neofluidics Llc A microfluidic serial dilution platform based well-plate using an oil-free immiscible phase driven by manual or electronic pipettors and method of operation
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN117098607A (en) 2021-02-24 2023-11-21 10X基因组学有限公司 Method for concentrating droplets in an emulsion
EP4313412A1 (en) 2021-03-26 2024-02-07 10X Genomics, Inc. Devices, methods, and systems for improved droplet recovery
EP4330421A1 (en) 2021-04-26 2024-03-06 The Brigham and Women's Hospital, Inc. Compositions and methods for characterizing polynucleotide sequence alterations
WO2022271848A1 (en) 2021-06-22 2022-12-29 Pneuma Respiratory, Inc. Droplet delivery device with push ejection
WO2023004068A2 (en) 2021-07-21 2023-01-26 10X Genomics, Inc. Methods, devices, and kits for purifying and lysing biological particles
WO2023021295A2 (en) 2021-08-18 2023-02-23 Nuclera Nucleics Ltd Methods and compositions for improved biomolecule assays on digital microfluidic devices
AU2022376541A1 (en) 2021-10-29 2024-04-18 Slingshot Biosciences, Inc. Hydrogel particles as feeder cells and as synthetic antigen presenting cells
WO2023099667A1 (en) 2021-12-01 2023-06-08 Vilnius University Methods for processing and barcoding nucleic acids
WO2023168423A1 (en) 2022-03-04 2023-09-07 10X Genomics, Inc. Droplet forming devices and methods having fluoropolymer silane coating agents
CN114643088B (en) * 2022-03-14 2024-04-19 常熟理工学院 Micro-droplet generation chip based on karman vortex street
WO2023215886A1 (en) 2022-05-05 2023-11-09 Slingshot Biosciences, Inc. Engineered particles as red blood cell mimics and compositions containing same for hematology
US20240026339A1 (en) 2022-07-10 2024-01-25 Vilnius University Composition and the use of cell lysis reagents
WO2024039763A2 (en) 2022-08-18 2024-02-22 10X Genomics, Inc. Droplet forming devices and methods having flourous diol additives
WO2024058036A1 (en) * 2022-09-14 2024-03-21 国立大学法人 鹿児島大学 Nozzle for bubble formation, bubble formation device, bubble formation method, and method for producing nozzle for bubble formation

Family Cites Families (163)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2692800A (en) 1951-10-08 1954-10-26 Gen Electric Nozzle flow control
US3980541A (en) 1967-06-05 1976-09-14 Aine Harry E Electrode structures for electric treatment of fluids and filters using same
US3816331A (en) 1972-07-05 1974-06-11 Ncr Continuous encapsulation and device therefor
CH563807A5 (en) * 1973-02-14 1975-07-15 Battelle Memorial Institute Fine granules and microcapsules mfrd. from liquid droplets - partic. of high viscosity requiring forced sepn. of droplets
CH564966A5 (en) * 1974-02-25 1975-08-15 Sauter Fr Ag Fabrik Elektrisch
US4059552A (en) 1974-06-21 1977-11-22 The Dow Chemical Company Cross-linked water-swellable polymer particles
US3982541A (en) 1974-07-29 1976-09-28 Esperance Jr Francis A L Eye surgical instrument
JPS5372016A (en) * 1976-12-08 1978-06-27 Toyo Tire & Rubber Co Ltd Apparatus for preparation and supply of heavy oil w/o emulsion fuel
US4279345A (en) 1979-08-03 1981-07-21 Allred John C High speed particle sorter using a field emission electrode
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
JPS6057907B2 (en) 1981-06-18 1985-12-17 工業技術院長 Liquid mixing and atomization method
DE3230289A1 (en) * 1982-08-14 1984-02-16 Bayer Ag, 5090 Leverkusen PRODUCTION OF PHARMACEUTICAL OR COSMETIC DISPERSIONS
US4853336A (en) 1982-11-15 1989-08-01 Technicon Instruments Corporation Single channel continuous flow system
US4618476A (en) * 1984-02-10 1986-10-21 Eastman Kodak Company Capillary transport device having speed and meniscus control means
US4865444A (en) * 1984-04-05 1989-09-12 Mobil Oil Corporation Apparatus and method for determining luminosity of hydrocarbon fuels
CA1235367A (en) * 1984-04-05 1988-04-19 Gary J. Green Method and apparatus for producing uniform liquid droplets
GB8604328D0 (en) 1986-02-21 1986-03-26 Ici Plc Producing spray of droplets of liquid
US4916070A (en) 1986-04-14 1990-04-10 The General Hospital Corporation Fibrin-specific antibodies and method of screening for the antibodies
US5204112A (en) 1986-06-16 1993-04-20 The Liposome Company, Inc. Induction of asymmetry in vesicles
US5149625A (en) 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US4931225A (en) * 1987-12-30 1990-06-05 Union Carbide Industrial Gases Technology Corporation Method and apparatus for dispersing a gas into a liquid
US5093602A (en) * 1989-11-17 1992-03-03 Charged Injection Corporation Methods and apparatus for dispersing a fluent material utilizing an electron beam
JP3176607B2 (en) 1990-02-07 2001-06-18 群馬大学長 Method for forming uniform droplets
US6149789A (en) 1990-10-31 2000-11-21 Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Process for manipulating microscopic, dielectric particles and a device therefor
DE4143573C2 (en) 1991-08-19 1996-07-04 Fraunhofer Ges Forschung Device for separating mixtures of microscopic dielectric particles suspended in a liquid or a gel
SE500071C2 (en) 1992-06-25 1994-04-11 Vattenfall Utveckling Ab Device for mixing two fluids, in particular liquids of different temperature
DE4308839C2 (en) * 1993-03-19 1997-04-30 Jordanow & Co Gmbh Device for mixing flow media
US5512131A (en) 1993-10-04 1996-04-30 President And Fellows Of Harvard College Formation of microstamped patterns on surfaces and derivative articles
ES2151007T3 (en) * 1994-06-13 2000-12-16 Praxair Technology Inc NARROW LIQUID FUEL SPRAY ATOMIZERS FOR COMBUSTION.
US5935331A (en) 1994-09-09 1999-08-10 Matsushita Electric Industrial Co., Ltd. Apparatus and method for forming films
US5762775A (en) 1994-09-21 1998-06-09 Lockheed Martin Energy Systems, Inc. Method for electrically producing dispersions of a nonconductive fluid in a conductive medium
JPH08153669A (en) 1994-11-30 1996-06-11 Hitachi Ltd Thin film forming method and formation device
EP0812434B1 (en) 1995-03-01 2013-09-18 President and Fellows of Harvard College Microcontact printing on surfaces and derivative articles
JP3232525B2 (en) 1995-08-22 2001-11-26 信越化学工業株式会社 Water repellent agent
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5851769A (en) 1995-09-27 1998-12-22 The Regents Of The University Of California Quantitative DNA fiber mapping
JP3759986B2 (en) 1995-12-07 2006-03-29 フロイント産業株式会社 Seamless capsule and manufacturing method thereof
US5681600A (en) * 1995-12-18 1997-10-28 Abbott Laboratories Stabilization of liquid nutritional products and method of making
US5868322A (en) 1996-01-31 1999-02-09 Hewlett-Packard Company Apparatus for forming liquid droplets having a mechanically fixed inner microtube
US6355198B1 (en) 1996-03-15 2002-03-12 President And Fellows Of Harvard College Method of forming articles including waveguides via capillary micromolding and microtransfer molding
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US6405936B1 (en) 1996-05-13 2002-06-18 Universidad De Sevilla Stabilized capillary microjet and devices and methods for producing same
US6299145B1 (en) 1996-05-13 2001-10-09 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6189803B1 (en) 1996-05-13 2001-02-20 University Of Seville Fuel injection nozzle and method of use
ES2140998B1 (en) 1996-05-13 2000-10-16 Univ Sevilla LIQUID ATOMIZATION PROCEDURE.
US6386463B1 (en) 1996-05-13 2002-05-14 Universidad De Sevilla Fuel injection nozzle and method of use
US6248378B1 (en) 1998-12-16 2001-06-19 Universidad De Sevilla Enhanced food products
US6196525B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for fluid aeration via gas forced through a liquid within an orifice of a pressure chamber
US6197835B1 (en) 1996-05-13 2001-03-06 Universidad De Sevilla Device and method for creating spherical particles of uniform size
US6187214B1 (en) 1996-05-13 2001-02-13 Universidad De Seville Method and device for production of components for microfabrication
WO1998000231A1 (en) 1996-06-28 1998-01-08 Caliper Technologies Corporation High-throughput screening assay systems in microscale fluidic devices
US6252129B1 (en) 1996-07-23 2001-06-26 Electrosols, Ltd. Dispensing device and method for forming material
US6143248A (en) * 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
DK0925494T3 (en) 1996-09-04 2002-07-01 Scandinavian Micro Biodevices Microfluidic system for particle separation and analysis
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
US6120666A (en) 1996-09-26 2000-09-19 Ut-Battelle, Llc Microfabricated device and method for multiplexed electrokinetic focusing of fluid streams and a transport cytometry method using same
JPH10217477A (en) 1997-02-07 1998-08-18 Fuji Xerox Co Ltd Ink jet recording device
ATE358177T1 (en) 1997-07-07 2007-04-15 Medical Res Council AN IN VITRO SORTING PROCESS
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
EP1023118B1 (en) * 1997-09-25 2003-07-02 GE Bayer Silicones GmbH & Co. KG Device and method for producing silicone emulsions
JP3081880B2 (en) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 Microsphere continuous manufacturing equipment
ATE530891T1 (en) 1998-05-22 2011-11-15 California Inst Of Techn MINIATURIZED CELL SORTER
US6003794A (en) 1998-08-04 1999-12-21 Progressive Grower Technologies, Inc. Electrostatic spray module
US6614598B1 (en) 1998-11-12 2003-09-02 Institute Of Technology, California Microlensing particles and applications
US6450189B1 (en) 1998-11-13 2002-09-17 Universidad De Sevilla Method and device for production of components for microfabrication
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6633031B1 (en) 1999-03-02 2003-10-14 Advion Biosciences, Inc. Integrated monolithic microfabricated dispensing nozzle and liquid chromatography-electrospray system and method
DE19911777A1 (en) * 1999-03-17 2000-09-21 Merck Patent Gmbh Process for the preparation of cosmetic formulations
US6592821B1 (en) 1999-05-17 2003-07-15 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
WO2000070080A1 (en) * 1999-05-17 2000-11-23 Caliper Technologies Corp. Focusing of microparticles in microfluidic systems
US20060169800A1 (en) * 1999-06-11 2006-08-03 Aradigm Corporation Aerosol created by directed flow of fluids and devices and methods for producing same
WO2000076673A1 (en) * 1999-06-11 2000-12-21 Aradigm Corporation Method for producing an aerosol
US7601270B1 (en) 1999-06-28 2009-10-13 California Institute Of Technology Microfabricated elastomeric valve and pump systems
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
US20010050881A1 (en) 1999-09-20 2001-12-13 Depaoli David W. Continuous flow, electrohydrodynamic micromixing apparatus and methods
US6890487B1 (en) * 1999-09-30 2005-05-10 Science & Technology Corporation ©UNM Flow cytometry for high throughput screening
DE19961257C2 (en) 1999-12-18 2002-12-19 Inst Mikrotechnik Mainz Gmbh micromixer
AU2001232805A1 (en) 2000-01-12 2001-07-24 Ut-Battelle, Llc A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
BR0109088A (en) * 2000-03-10 2003-05-20 Flow Focusing Inc Methods for Fiber Optic Production by High Viscosity Liquid Focusing
US7485454B1 (en) * 2000-03-10 2009-02-03 Bioprocessors Corp. Microreactor
DE10015109A1 (en) * 2000-03-28 2001-10-04 Peter Walzel Processes and devices for producing drops of equal size
EP1282910B1 (en) 2000-04-18 2017-04-05 Waters Technologies Corporation Improved electrospray and other lc/ms interfaces
JP2001301154A (en) 2000-04-20 2001-10-30 Dainippon Printing Co Ltd Field jet sticking method of liquid having surface tension lowering upon application of voltage
DE10025290B4 (en) 2000-05-22 2005-03-24 Fico I.T.M. S.A. Sun visor outer surfaces
US20010048637A1 (en) * 2000-05-24 2001-12-06 Weigl Bernhard H. Microfluidic system and method
US6645432B1 (en) 2000-05-25 2003-11-11 President & Fellows Of Harvard College Microfluidic systems including three-dimensionally arrayed channel networks
US6686184B1 (en) 2000-05-25 2004-02-03 President And Fellows Of Harvard College Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks
US6777450B1 (en) 2000-05-26 2004-08-17 Color Access, Inc. Water-thin emulsions with low emulsifier levels
US20060263888A1 (en) 2000-06-02 2006-11-23 Honeywell International Inc. Differential white blood count on a disposable card
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6301055B1 (en) 2000-08-16 2001-10-09 California Institute Of Technology Solid immersion lens structures and methods for producing solid immersion lens structures
DE10041823C2 (en) 2000-08-25 2002-12-19 Inst Mikrotechnik Mainz Gmbh Method and static micromixer for mixing at least two fluids
US6610499B1 (en) * 2000-08-31 2003-08-26 The Regents Of The University Of California Capillary array and related methods
WO2002023163A1 (en) * 2000-09-15 2002-03-21 California Institute Of Technology Microfabricated crossflow devices and methods
US6508988B1 (en) 2000-10-03 2003-01-21 California Institute Of Technology Combinatorial synthesis system
US6778724B2 (en) 2000-11-28 2004-08-17 The Regents Of The University Of California Optical switching and sorting of biological samples and microparticles transported in a micro-fluidic device, including integrated bio-chip devices
US20040096515A1 (en) 2001-12-07 2004-05-20 Bausch Andreas R. Methods and compositions for encapsulating active agents
EP1385488A2 (en) 2000-12-07 2004-02-04 President And Fellows Of Harvard College Methods and compositions for encapsulating active agents
ES2180405B1 (en) 2001-01-31 2004-01-16 Univ Sevilla DEVICE AND PROCEDURE FOR PRODUCING MULTICOMPONENT COMPOSITE LIQUID JEANS AND MULTICOMPONENT AND / OR MULTI-PAPER MICRO AND NANOMETRIC SIZE CAPSULES.
EP1355537A4 (en) 2001-01-31 2010-04-07 Kraft Foods Global Brands Llc Production of capsules and particles for improvement of food products
EP1741482B1 (en) 2001-02-23 2008-10-15 Japan Science and Technology Agency Process and apparatus for producing microcapsules
US7268167B2 (en) 2001-02-23 2007-09-11 Japan Science And Technology Agency Process for producing emulsion and microcapsules and apparatus therefor
US7037417B2 (en) * 2001-03-19 2006-05-02 Ecole Polytechnique Federale De Lausanne Mechanical control of fluids in micro-analytical devices
US6752922B2 (en) 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US7318642B2 (en) 2001-04-10 2008-01-15 Essilor International (Compagnie Générale d'Optique) Progressive addition lenses with reduced unwanted astigmatism
JP4194934B2 (en) 2001-05-26 2008-12-10 ワン セル システムズ インコーポレイテッド Protein secretion by encapsulated cells
GB0114854D0 (en) 2001-06-18 2001-08-08 Medical Res Council Selective gene amplification
US20030015425A1 (en) 2001-06-20 2003-01-23 Coventor Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
EP1412065A2 (en) 2001-07-27 2004-04-28 President And Fellows Of Harvard College Laminar mixing apparatus and methods
US6520425B1 (en) 2001-08-21 2003-02-18 The University Of Akron Process and apparatus for the production of nanofibers
JP4182195B2 (en) * 2001-09-03 2008-11-19 独立行政法人農業・食品産業技術総合研究機構 Monodispersed complex emulsion production equipment
US7147763B2 (en) 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
US6976590B2 (en) 2002-06-24 2005-12-20 Cytonome, Inc. Method and apparatus for sorting particles
JP4855680B2 (en) 2002-05-09 2012-01-18 ザ・ユニバーシティ・オブ・シカゴ Apparatus and method for transport and reaction by pressure driven plug
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
GB2395196B (en) 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application
WO2004071638A2 (en) 2003-02-11 2004-08-26 Regents Of The University Of California, The Microfluidic devices and method for controlled viscous shearing and formation of amphiphilic vesicles
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US7045040B2 (en) 2003-03-20 2006-05-16 Asm Nutool, Inc. Process and system for eliminating gas bubbles during electrochemical processing
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
EP3616781A1 (en) 2003-04-10 2020-03-04 President and Fellows of Harvard College Formation and control of fluidic species
EP1629286A1 (en) 2003-05-16 2006-03-01 Global Technologies (NZ) Ltd. Method and apparatus for mixing sample and reagent in a suspension fluid
WO2004103565A2 (en) 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Device and method for structuring liquids and for dosing reaction liquids into liquid compartments immersed in a separation medium
JP2005037346A (en) 2003-06-25 2005-02-10 Aisin Seiki Co Ltd Micro fluid control system
US7115230B2 (en) 2003-06-26 2006-10-03 Intel Corporation Hydrodynamic focusing devices
GB0315438D0 (en) 2003-07-02 2003-08-06 Univ Manchester Analysis of mixed cell populations
US20050032238A1 (en) 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
EP1658133A1 (en) 2003-08-27 2006-05-24 President And Fellows Of Harvard College Electronic control of fluidic species
CN1860363B (en) 2003-08-28 2011-12-28 赛路拉公司 Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
US7204431B2 (en) 2003-10-31 2007-04-17 Agilent Technologies, Inc. Electrospray ion source for mass spectroscopy
US20050103690A1 (en) 2003-11-19 2005-05-19 Aisin Seiki Kabushiki Kaisha Micro liquid control system
WO2005049787A2 (en) 2003-11-24 2005-06-02 Yeda Research And Development Co.Ltd. Compositions and methods for in vitro sorting of molecular and cellular libraries
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US8696952B2 (en) 2004-04-23 2014-04-15 Eugenia Kumacheva Method of producing polymeric particles with selected size, shape, morphology and composition
EP1796828A1 (en) 2004-07-02 2007-06-20 VersaMatrix A/S Spherical radiofrequency-encoded beads
US9477233B2 (en) 2004-07-02 2016-10-25 The University Of Chicago Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets
US7759111B2 (en) 2004-08-27 2010-07-20 The Regents Of The University Of California Cell encapsulation microfluidic device
CN101052468B (en) 2004-09-09 2012-02-01 居里研究所 Microfluidic device using a collinear electric field
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006051552A2 (en) 2004-11-15 2006-05-18 Yeda Research And Development Co. Ltd. At The Weizmann Institute Of Science Directed evolution and selection using in vitro compartmentalization
WO2006078841A1 (en) 2005-01-21 2006-07-27 President And Fellows Of Harvard College Systems and methods for forming fluidic droplets encapsulated in particles such as colloidal particles
US20070054119A1 (en) 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
JP2008535644A (en) 2005-03-04 2008-09-04 プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ Method and apparatus for the formation of multiple emulsions
FR2882939B1 (en) * 2005-03-11 2007-06-08 Centre Nat Rech Scient FLUIDIC SEPARATION DEVICE
US8734003B2 (en) 2005-09-15 2014-05-27 Alcatel Lucent Micro-chemical mixing
US20100137163A1 (en) 2006-01-11 2010-06-03 Link Darren R Microfluidic Devices and Methods of Use in The Formation and Control of Nanoreactors
WO2007087312A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Molecular counting
JP2009524825A (en) 2006-01-27 2009-07-02 プレジデント アンド フェロウズ オブ ハーバード カレッジ Fluid droplet coalescence
US20090181864A1 (en) 2006-03-31 2009-07-16 Nam Trung Nguyen Active control for droplet-based microfluidics
US20080003142A1 (en) 2006-05-11 2008-01-03 Link Darren R Microfluidic devices
FR2901717A1 (en) 2006-05-30 2007-12-07 Centre Nat Rech Scient METHOD FOR TREATING DROPS IN A MICROFLUIDIC CIRCUIT
EP2136786B8 (en) 2007-03-28 2012-11-14 President and Fellows of Harvard College Apparatus for forming droplets
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
US20090068170A1 (en) 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
WO2010009365A1 (en) 2008-07-18 2010-01-21 Raindance Technologies, Inc. Droplet libraries
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
JP2010198393A (en) 2009-02-26 2010-09-09 Alpine Electronics Inc Map display device
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding

Also Published As

Publication number Publication date
CN1678397A (en) 2005-10-05
US20140037514A1 (en) 2014-02-06
CN102059162A (en) 2011-05-18
AU2003253751B2 (en) 2009-10-08
EP1515803A2 (en) 2005-03-23
AU2003253751A1 (en) 2004-01-19
US8986628B2 (en) 2015-03-24
US7708949B2 (en) 2010-05-04
CA2491564A1 (en) 2004-01-08
EP2275206A1 (en) 2011-01-19
CN1678397B (en) 2011-02-09
US8337778B2 (en) 2012-12-25
WO2004002627A2 (en) 2004-01-08
US20050172476A1 (en) 2005-08-11
WO2004002627A3 (en) 2004-04-01
WO2004002627B1 (en) 2004-06-17
US20100172803A1 (en) 2010-07-08
JP2010075927A (en) 2010-04-08
JP2006507921A (en) 2006-03-09
CA2491564C (en) 2013-03-19
JP5624310B2 (en) 2014-11-12

Similar Documents

Publication Publication Date Title
WO2004002627A8 (en) Method and apparatus for fluid dispersion
GB2397256B (en) Apparatus and methods for microfluidic applications
AU2002360239A1 (en) Fluid processing device and method
AU2003280800A1 (en) Micro fluid control device and process for producing the same
AU2001272500A1 (en) Micromachined fluidic device and method for making same
AU2001232805A1 (en) A microfluidic device and method for focusing, segmenting, and dispensing of a fluid stream
WO2005043123A3 (en) System and apparatus for body fluid analysis using surface-textured optical materials
WO2003036265A3 (en) Assay systems with adjustable fluid communication
AU2003218806A1 (en) Bio-disc, bio-driver apparatus, and assay method using the same
ZA200402217B (en) Device and method for joining the end faces of parts.
AU2003232031A1 (en) Fluid mixing and irradiation device and method of using the device especially for biological fluids
AU2003253308A1 (en) Device and method for perforating web-like materials______
EP1880765A3 (en) Microfluidic system
WO2004098757A3 (en) Microfluidic mixing using flow pulsing
AU2001250929A1 (en) Method and apparatus for acoustically controlling liquid solutions in microfluidic devices
AU2002320463A1 (en) Self-regulating microfluidic device and method of using the same
CA2450879A1 (en) Apparatus for and method of radial expansion of a tubular member
AU2003260153A1 (en) Method and device for the extraction of materials from liquids or solid dispersions
AU2003297623A1 (en) Nanostructure based microfluidic pumping device and method
AU2002302393A1 (en) Fluid container, its manufacturing method and apparatus
AU2002342499A1 (en) Device and method for the transfer of liquid samples
AU2003216713A1 (en) Shaking device and method, particularly for dispersing or emulsifying two immiscible fluids
AU2001274919A1 (en) Novel method and apparatus for flow monitoring in micro-fluidic devices
AU2002347151A1 (en) Personal and environmental fluid sampling apparatus
AU2002337054A1 (en) Method and device for the through-flow sterilisation of liquids

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
B Later publication of amended claims

Effective date: 20040406

WWE Wipo information: entry into national phase

Ref document number: 2003253751

Country of ref document: AU

Ref document number: 537478

Country of ref document: NZ

Ref document number: 2004549845

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 11024228

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2491564

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003762228

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 20038204940

Country of ref document: CN

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 02/2004 UNDER (22) REPLACE "03 JUNE 2003 (03.06.03)" BY "03 JUNE 2003 (30.06.03)"

WWP Wipo information: published in national office

Ref document number: 2003762228

Country of ref document: EP