WO2007002890A3 - Methods of producing and sequencing modified polynucleotides - Google Patents

Methods of producing and sequencing modified polynucleotides Download PDF

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Publication number
WO2007002890A3
WO2007002890A3 PCT/US2006/025529 US2006025529W WO2007002890A3 WO 2007002890 A3 WO2007002890 A3 WO 2007002890A3 US 2006025529 W US2006025529 W US 2006025529W WO 2007002890 A3 WO2007002890 A3 WO 2007002890A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
producing
modified polynucleotides
phosphorothiolate linkage
sequencing modified
Prior art date
Application number
PCT/US2006/025529
Other languages
French (fr)
Other versions
WO2007002890A2 (en
Inventor
Douglas R Smith
Kevin J Mckernan
Original Assignee
Agencourt Personal Genomics Co
Douglas R Smith
Kevin J Mckernan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agencourt Personal Genomics Co, Douglas R Smith, Kevin J Mckernan filed Critical Agencourt Personal Genomics Co
Priority to EP06785931A priority Critical patent/EP1907584A2/en
Priority to JP2008519600A priority patent/JP2008546424A/en
Publication of WO2007002890A2 publication Critical patent/WO2007002890A2/en
Publication of WO2007002890A3 publication Critical patent/WO2007002890A3/en
Priority to US12/632,740 priority patent/US20100297628A1/en
Priority to US12/632,713 priority patent/US8058030B2/en
Priority to US12/632,730 priority patent/US20100298551A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

The present invention encompasses methods for producing a modified polynucleotide sequence that comprises a (e.g., one or more) phosphorothiolate linkage, methods for determining a polynucleotide sequence comprising a (e.g., one or more) phosphorothiolate linkage, and methods for separating forward and reverse extension products that comprise a (e.g., one or more) phosphorothiolate linkage. The invention also encompasses kits for producing and/or determining the sequence of a modified polynucleotide that comprises a (e.g., one or more) phosphorothiolate linkage.
PCT/US2006/025529 2005-06-28 2006-06-28 Methods of producing and sequencing modified polynucleotides WO2007002890A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP06785931A EP1907584A2 (en) 2005-06-28 2006-06-28 Methods of producing and sequencing modified polynucleotides
JP2008519600A JP2008546424A (en) 2005-06-28 2006-06-28 Methods for making and sequencing modified polynucleotides
US12/632,740 US20100297628A1 (en) 2005-06-28 2009-12-07 Methods Of Producing And Sequencing Modified Polynucleotides
US12/632,713 US8058030B2 (en) 2005-06-28 2009-12-07 Methods of producing and sequencing modified polynucleotides
US12/632,730 US20100298551A1 (en) 2005-06-28 2009-12-07 Methods Of Producing And Sequencing Modified Polynucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69478305P 2005-06-28 2005-06-28
US60/694,783 2005-06-28

Publications (2)

Publication Number Publication Date
WO2007002890A2 WO2007002890A2 (en) 2007-01-04
WO2007002890A3 true WO2007002890A3 (en) 2007-06-28

Family

ID=37596075

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/025529 WO2007002890A2 (en) 2005-06-28 2006-06-28 Methods of producing and sequencing modified polynucleotides

Country Status (4)

Country Link
US (4) US7645866B2 (en)
EP (1) EP1907584A2 (en)
JP (2) JP2008546424A (en)
WO (1) WO2007002890A2 (en)

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US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8845880B2 (en) 2010-12-22 2014-09-30 Genia Technologies, Inc. Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
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US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
WO2014126937A1 (en) * 2013-02-14 2014-08-21 The Board Of Trustees Of The Leland Stanford Junior University Suspension arrays and multiplexed assays based thereon
US20160145679A1 (en) * 2013-03-01 2016-05-26 Arizona Board Of Regents On Behalf Of Arizona State University Oligonucleotide functionalized quantum dots
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
CA2926138A1 (en) 2013-10-23 2015-04-30 Genia Technologies, Inc. High speed molecular sensing with nanopores
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
JP6673843B2 (en) 2013-11-17 2020-03-25 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ Optical systems and assay chips for probing, detecting and analyzing molecules
WO2015167972A1 (en) * 2014-04-28 2015-11-05 Tsavachidou Dimitra Methods for nucleic acid base determination
JP6707520B2 (en) 2014-08-08 2020-06-10 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ Integrated device for time binning of received photons
BR112017002489B1 (en) 2014-08-08 2023-02-14 Quantum-Si Incorporated INSTRUMENT CONFIGURED TO INTERFACE A TEST CHIP, APPARATUS, METHOD OF ANALYZING A SPECIMEN, METHOD OF SEQUENCING A TARGET MOLECULE OF NUCLEIC ACID, AND METHOD OF SEQUENCING NUCLEIC ACID
CA2957546A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Integrated device with external light source for probing, detecting, and analyzing molecules
US10303666B2 (en) 2015-03-09 2019-05-28 International Business Machines Corporation File transfer system using file backup times
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
PL415967A1 (en) * 2016-01-29 2017-07-31 Univ Warszawski 5'-thiophosphate analogs of 5' mRNA (capu) end, method for obtaining them and applications
TWI734748B (en) 2016-02-17 2021-08-01 美商太斯萊特健康股份有限公司 Sensor and device for lifetime imaging and detection applications
JP7149275B2 (en) 2016-12-22 2022-10-06 クアンタム－エスアイ インコーポレイテッド Integrated photodetector with direct binning pixels
US11391626B2 (en) 2018-06-22 2022-07-19 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time

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Also Published As

Publication number Publication date
JP2008546424A (en) 2008-12-25
US20100297628A1 (en) 2010-11-25
JP2011155986A (en) 2011-08-18
US7645866B2 (en) 2010-01-12
US20100285461A1 (en) 2010-11-11
US20070026438A1 (en) 2007-02-01
EP1907584A2 (en) 2008-04-09
WO2007002890A2 (en) 2007-01-04
US8058030B2 (en) 2011-11-15
US20100298551A1 (en) 2010-11-25

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