WO2010090645A1 - A new generation of fluorescent microbead cellular surrogate standards - Google Patents

A new generation of fluorescent microbead cellular surrogate standards Download PDF

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Publication number
WO2010090645A1
WO2010090645A1 PCT/US2009/033519 US2009033519W WO2010090645A1 WO 2010090645 A1 WO2010090645 A1 WO 2010090645A1 US 2009033519 W US2009033519 W US 2009033519W WO 2010090645 A1 WO2010090645 A1 WO 2010090645A1
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Prior art keywords
populations
microbead
microbeads
fluorescent
intensity profiles
Prior art date
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PCT/US2009/033519
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French (fr)
Inventor
Abraham Schwartz
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Abraham Schwartz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to PCT/US2009/033519 priority Critical patent/WO2010090645A1/en
Publication of WO2010090645A1 publication Critical patent/WO2010090645A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G01N21/278—Constitution of standards
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2015/1014—

Definitions

  • the present invention relates to microbead populations, and more particularly to a method of producing microbead populations that mimic the fluorescence intensity profile distribution of fluorescent biological cells so that they may be used as a standard for flow cytometry.
  • Microbeads have been used as surrogate cell standards in various biological fields for many years. This is especially true since highly uniform microbeads, in the 2 - 15 micron diameter range, have been first produced by John Ugelstad.
  • polymeric microbeads As a particle surrogate, polymeric microbeads have high physical stability, as well as the ability to be labeled with the same fluorochromes and dyes as used to label biological cells.
  • a great deal of work has been addressed to make microbeads useful as fluorescent particle reference and quantitative standards. When labeled with such fluorochromes and dyes, these microbeads exhibit many of characteristics as labeled biological cells, as determined by instrumentation of at least one of flow cytometry.
  • fluorochrome labeled microbeads appear to have similar forward and side light scatter properties, as well as similar spectral and intensity fluorescent properties as biological cells.
  • fluorescence intensity distribution profile of microbeads and biological cells labeled with the same fluorochrome can appear vastly different.
  • human lymphocytes labeled with a fluorescently conjugated CD8 antibody has a fluorescence intensity distribution, as shown in FIG 1. This distribution is really the combined response of CD8 suppressor cells, the tall intense population on the right end of the distribution, and CD8 cyto-toxic cells, the low widely spread population to the left of the CD8 suppressor cells.
  • the present invention solves this need by providing a method to manipulate a population of microbeads labeled with a fluorescent material or optical dye so that it mimics the fluorescent intensity distribution of a biological cell population labeled with the same fluorescent material or dye.
  • This may be accomplished by a number of ways which include, but are not limited to at least one of: 1) mixing microbead populations of different intensities and uniformities, 2) manipulating the method of addition of the fluorescent material or 3) performing precision photo bleaching of a uniform population of microbeads.
  • the present invention provides method to manipulate a population of microbeads labeled with a fluorescent material or dye so that it mimics the fluorescent intensity distribution of a biological cell population labeled with the same fluorescent material or optical dye, by mixing different uniform populations of microbeads labeled with a fluorescent material in specific proportions, the overall fluorescent intensity pattern of the mixture can result in the same intensity pattern of the a cell population of interest labeled with the same material.
  • the intensity profile of a labeled cell population is also mimicked by carefully adjusting the physical parameters during the dying or polymerization procedure of the microbeads. Namely, adjusting the rate of dye addition, the stirring conditions and varying concentrations of the fluorescent material during the process.
  • a fluorescence intensity profile of a microbead population is accomplished using a process referred to as photo bleaching. That is, when fluorescent materials are exposed to strong light, especially wavelengths within their absorption spectra, the fluorescent molecules undergo destruction at some intrinsic rate, resulting in lower fluorescent intensity of the population as a whole. However, if different portions of the uniform population of microbeads are photobleached in a precise and predetermined manner at specific rates, the resulting intensity profile of the microbead population can be made to mimic the intensity profile of the labeled biological cells. This process is referred to as precision photo bleaching.
  • FIG. 1 shows a fluorescence intensity distribution of CD8 labeled with a fluorescent CD8 antibody.
  • FIG. 2 shows a fluorescence intensity distribution microbeads labeled with the same fluorochrome as bound to the CD8 antibody in Fig. 1.
  • FIG. 3 shows a plot of an intensity distribution from FIG. 9
  • FIG. 4 shows a plot of the inverted intensity distribution percentile from FIG.
  • FIG. 5 shows a plot of the sorted inverted intensity distribution percentile from
  • FIG. 6 shows the density gradient used to perform precision photo bleaching.
  • FIG. 7 shows the apparatus used to photo bleach a population of microbeads according to the present invention.
  • FIG. 8 shows fluorescence intensity profiles of a microbead population (a) before and (b) after photo bleaching through a optical density gradient.
  • FIG. 9 shows data from a list mode file indicating the number of events in each intensity channel of the histogram.
  • FIG. IO shows data from FIG. 9 that has been inverted.
  • FIG. 11 shows data from FIG. 10 that has been normalized to a density percentage.
  • FIG. 12 shows normalized data from FIG. 11 that has been sorted.
  • the preferred embodiment of the invention involves a method, an algorithm, and a precision photo bleaching apparatus for the production of microbeads that provide specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cells.
  • the microbeads are uniformly spread over a given area. This may be done by filling a shallow flat-bottomed container and allowing the microbeads to settle uniformly onto the bottom of the container. An optical density gradient is placed over these microbeads and a light source of optimal wavelengths is directed through density gradient onto the uniform layer of microbead. The intensity and length of time the light is directed onto the microbeads controls the overall fluorescence intensity of the population, however, the intensity profile within the microbead population will retain an intensity profile across the illuminated area inversely proportional to the density gradient covering the microbeads.
  • the microbead surrogate cell standard is produced from a profile of an actual fluorescently labeled biological cell population by obtaining a list mode file from the cell population of interest with a flow cytometer.
  • the channel number and correlated event data from the list mode file is then transferred to a spreadsheet indicating the number of events represented in each channel of the intensity scale as shown in FIG. 9.
  • This intensity profile is then inverted by subtracting the number of events in each channel from the value in the channel with the maximum events as shown in FIG 10.
  • These inverted event values are then numerically sorted as shown in FIG. 11.
  • This sorted list is then converted to a percent of the maximum value as shown in FIG. 12.
  • These converted sorted numbers are then used to generate a density gradient as shown in FIG. 3, through which a uniform light source is directed onto a uniform layer of microbeads so that areas of the microbeads will photo bleach to a relative value inversely proportional to the density gradient covering them as shown in FIG. 6.
  • Example 2 Controlling fluorescence intensity distribution during microbead dying procedure.
  • propidum iodide dissolved in methanol was introduced at the top of the microbead suspension without stirring. The PI was allowed to diffused down through the suspension while the microbeads were settling to the bottom. After 30 minutes, the methanol was decanted from the microbeads and they were re- suspended in PBS containing 0.1% Tween 20. The fluorescence intensity distribution of the microbead population was found to have a wide distribution skewed to the left, >60%CV.
  • a highly uniform population of fluorescein-labeled microbeads was allowed to settle over an area so that the resulting microbeads formed a uniformly thick layer. Most of the suspension solution was removed so that the microbeads were covered by only a few millimeters of solution.
  • An optical density gradient produced by applying the algorithm to a list mode histogram file from gated CD8 FITC labeled lymphocytes. This gradient was placed 5 mm above the uniform layer of microbeads and a 5Ow high intensity lamp was directed onto the gradient for 2 hours so that the layer of microbeads was exposed to the resulting gradient light levels. The microbeads were then re-suspended and washed in PBS containing 0.1% Tween 20. The resulting fluorescent intensity histogram of the precision photobleached microbeads mimicked the gated CD8 FITC labeled lymphocytes

Abstract

A method of producing microbead populations that mimics the fluorescence intensity profile distribution of fluorescent biological cells so that they may be used a standard for flow cytometry.

Description

A NEW GENERATION OF FLUORESCENT MICROBEAD CELLULAR SURROGATE STANDARDS
INVENTOR
Abraham Schwartz, San Juan, Puerto Rico
FIELD OF THE INVENTION
The present invention relates to microbead populations, and more particularly to a method of producing microbead populations that mimic the fluorescence intensity profile distribution of fluorescent biological cells so that they may be used as a standard for flow cytometry.
BACKGROUND OF THE INVENTION
Microbeads have been used as surrogate cell standards in various biological fields for many years. This is especially true since highly uniform microbeads, in the 2 - 15 micron diameter range, have been first produced by John Ugelstad. As a particle surrogate, polymeric microbeads have high physical stability, as well as the ability to be labeled with the same fluorochromes and dyes as used to label biological cells. Moreover, a great deal of work has been addressed to make microbeads useful as fluorescent particle reference and quantitative standards. When labeled with such fluorochromes and dyes, these microbeads exhibit many of characteristics as labeled biological cells, as determined by instrumentation of at least one of flow cytometry. Specifically, fluorochrome labeled microbeads appear to have similar forward and side light scatter properties, as well as similar spectral and intensity fluorescent properties as biological cells. However, the fluorescence intensity distribution profile of microbeads and biological cells labeled with the same fluorochrome can appear vastly different. For example human lymphocytes labeled with a fluorescently conjugated CD8 antibody, has a fluorescence intensity distribution, as shown in FIG 1. This distribution is really the combined response of CD8 suppressor cells, the tall intense population on the right end of the distribution, and CD8 cyto-toxic cells, the low widely spread population to the left of the CD8 suppressor cells.
Much effort in the manufacture of microbeads is focused on producing highly uniform physical properties, of at least one of size, volume and fluorescence intensity. Such populations are highly sort after for use as size and fluorescence standards in fields of at least one of flow cytometry and fluorescence microscopy. Normally, these methods of production ensure that fluorochromes or dyes are taken up by the microbeads in a highly uniform fashion resulting in a very tight fluorescence intensity distribution, as shown in FIG.2.
However, it stands to reason that, the closer the standards mimic analyte being measured, the better the instrument performance can be evaluated to measure the analyte. An example to illustrate this may be found when counting CD8 labeled lymphocytes. If a fluorescent population of microbeads with the usual tight intensity distribution is used as the count standard, the standard is only determining the ability of the instrument to detect and count events in a narrow range of the intensity scale, as shown in FIG.2. However, as seen in FIG. 1, the intensity distribution of CD8 labeled lymphocytes cover a wide non-uniform intensity range. Although highly uniform intensity microbeads have served the biology and medical communities as cellular surrogates and standards well for a long time, the ability of microbeads that more closely resemble the intensity distributions of biological cells would be very welcome to these communities.
SUMMARY OF THE INVENTION
The present invention solves this need by providing a method to manipulate a population of microbeads labeled with a fluorescent material or optical dye so that it mimics the fluorescent intensity distribution of a biological cell population labeled with the same fluorescent material or dye. This may be accomplished by a number of ways which include, but are not limited to at least one of: 1) mixing microbead populations of different intensities and uniformities, 2) manipulating the method of addition of the fluorescent material or 3) performing precision photo bleaching of a uniform population of microbeads.
To achieve the foregoing and other advantages, the present invention, briefly described, provides method to manipulate a population of microbeads labeled with a fluorescent material or dye so that it mimics the fluorescent intensity distribution of a biological cell population labeled with the same fluorescent material or optical dye, by mixing different uniform populations of microbeads labeled with a fluorescent material in specific proportions, the overall fluorescent intensity pattern of the mixture can result in the same intensity pattern of the a cell population of interest labeled with the same material.
The intensity profile of a labeled cell population is also mimicked by carefully adjusting the physical parameters during the dying or polymerization procedure of the microbeads. Namely, adjusting the rate of dye addition, the stirring conditions and varying concentrations of the fluorescent material during the process.
Further another method of adjusting a fluorescence intensity profile of a microbead population is accomplished using a process referred to as photo bleaching. That is, when fluorescent materials are exposed to strong light, especially wavelengths within their absorption spectra, the fluorescent molecules undergo destruction at some intrinsic rate, resulting in lower fluorescent intensity of the population as a whole. However, if different portions of the uniform population of microbeads are photobleached in a precise and predetermined manner at specific rates, the resulting intensity profile of the microbead population can be made to mimic the intensity profile of the labeled biological cells. This process is referred to as precision photo bleaching.
Numerous objects, features and advantages of the present embodiment of the invention will be readily apparent to those of ordinary skill in the art upon a reading of the following detailed description of presently preferred, but nonetheless illustrative, embodiments of the invention when taken in conjunction with the accompanying drawings. In this respect, it is to be understood that the embodiment of the invention is not limited in its application to the details of construction and to the arrangements of the components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments and of being practiced and carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein are for the purpose of descriptions and should not be regarded as limiting. As such, those skilled in the art will appreciate that the conception, upon which this disclosure is based, may readily be utilized as a basis for the designing of other structures, methods and systems for carrying out the several purposes of the present invention. It is important, therefore, that the claims be regarded as including such equivalent constructions insofar as they do not depart from the spirit and scope of the present embodiment of the invention.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a fluorescence intensity distribution of CD8 labeled with a fluorescent CD8 antibody.
FIG. 2 shows a fluorescence intensity distribution microbeads labeled with the same fluorochrome as bound to the CD8 antibody in Fig. 1.
FIG. 3 shows a plot of an intensity distribution from FIG. 9
FIG. 4 shows a plot of the inverted intensity distribution percentile from FIG.
10.
FIG. 5 shows a plot of the sorted inverted intensity distribution percentile from
FIG. 11.
FIG. 6 shows the density gradient used to perform precision photo bleaching.
FIG. 7 shows the apparatus used to photo bleach a population of microbeads according to the present invention.
FIG. 8 shows fluorescence intensity profiles of a microbead population (a) before and (b) after photo bleaching through a optical density gradient.
FIG. 9 shows data from a list mode file indicating the number of events in each intensity channel of the histogram.
FIG. IO shows data from FIG. 9 that has been inverted. FIG. 11 shows data from FIG. 10 that has been normalized to a density percentage.
FIG. 12 shows normalized data from FIG. 11 that has been sorted.
DESCRIPTION OF THE INVENTION
This specification and the accompanying figures disclose the preferred embodiment as example of the invention. The drawings illustrated in the figures are not to scale and are only intended to serve as illustrating examples of the invention. The invention is not intended to be limited to the embodiment illustrated. Numerous modifications, changes, variations, substitutions and equivalents will be apparent to those skilled in the art without departing from the spirit and scope of the present invention.
Referring now to FIG. 8, the preferred embodiment of the invention involves a method, an algorithm, and a precision photo bleaching apparatus for the production of microbeads that provide specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cells.
In general, the microbeads are uniformly spread over a given area. This may be done by filling a shallow flat-bottomed container and allowing the microbeads to settle uniformly onto the bottom of the container. An optical density gradient is placed over these microbeads and a light source of optimal wavelengths is directed through density gradient onto the uniform layer of microbead. The intensity and length of time the light is directed onto the microbeads controls the overall fluorescence intensity of the population, however, the intensity profile within the microbead population will retain an intensity profile across the illuminated area inversely proportional to the density gradient covering the microbeads.
More specifically, the microbead surrogate cell standard is produced from a profile of an actual fluorescently labeled biological cell population by obtaining a list mode file from the cell population of interest with a flow cytometer. The channel number and correlated event data from the list mode file is then transferred to a spreadsheet indicating the number of events represented in each channel of the intensity scale as shown in FIG. 9. This intensity profile is then inverted by subtracting the number of events in each channel from the value in the channel with the maximum events as shown in FIG 10. These inverted event values are then numerically sorted as shown in FIG. 11. This sorted list is then converted to a percent of the maximum value as shown in FIG. 12. These converted sorted numbers are then used to generate a density gradient as shown in FIG. 3, through which a uniform light source is directed onto a uniform layer of microbeads so that areas of the microbeads will photo bleach to a relative value inversely proportional to the density gradient covering them as shown in FIG. 6.
Examples
Example 1. Mixing fluorescent microbead populations
Mix together proportions of a series fluorescence microbead populations labeled with phycoerythrin (PE) of increasingly fluorescence intensity by a factor of 0.3 decades of the same size that have wide intensity distributions, e.g., >25%. Suspend this mixture in a diluent containing 0.1% Tween 20 and run this mixture in a flow cytometer, gate on the singlet to obtain a FL2 histogram. The resulting histogram mimics the low intensity population of CD8 labeled PE lymphocytes.
Example 2. Controlling fluorescence intensity distribution during microbead dying procedure.
After swelling polymethyl methacrylate microbeads with 100% methanol, propidum iodide (PI) dissolved in methanol was introduced at the top of the microbead suspension without stirring. The PI was allowed to diffused down through the suspension while the microbeads were settling to the bottom. After 30 minutes, the methanol was decanted from the microbeads and they were re- suspended in PBS containing 0.1% Tween 20. The fluorescence intensity distribution of the microbead population was found to have a wide distribution skewed to the left, >60%CV.
Example 3. Precision photobleaching
A highly uniform population of fluorescein-labeled microbeads was allowed to settle over an area so that the resulting microbeads formed a uniformly thick layer. Most of the suspension solution was removed so that the microbeads were covered by only a few millimeters of solution. An optical density gradient produced by applying the algorithm to a list mode histogram file from gated CD8 FITC labeled lymphocytes. This gradient was placed 5 mm above the uniform layer of microbeads and a 5Ow high intensity lamp was directed onto the gradient for 2 hours so that the layer of microbeads was exposed to the resulting gradient light levels. The microbeads were then re-suspended and washed in PBS containing 0.1% Tween 20. The resulting fluorescent intensity histogram of the precision photobleached microbeads mimicked the gated CD8 FITC labeled lymphocytes
While a preferred embodiment of the invention has been described in detail, it should be apparent that modifications and variations thereto are possible, all of which fall within the true spirit and scope of the invention.
With respect to the above description then, it is to be realized that the optimum dimensional relationships for the parts of the invention, to include variations in size, materials, shape, form, function and manner of operation, assembly and use, are deemed readily apparent and obvious to one skilled in the art, and all equivalent relationships to those illustrated in the drawings and described in the specification are intended to be encompassed by the present embodiment of the invention.
Therefore, the foregoing is considered as illustrative only of the principles of the embodiment of the invention. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the embodiment of the invention to the exact construction and operation shown and described, and accordingly, all suitable modifications and equivalents may be resorted to, falling within the scope of the embodiment of the invention.

Claims

What is claimed is:
Claim 1. Microbead populations comprising specific fluorescent intensity profiles so that they mimic fluorescence intensity profiles of labeled biological cell populations that can be produced by at least one of: during manufacture or physical manipulating after production of the fluorescent microbead populations.
Claim 2. The Microbead populations of Claim 1, wherein the specific fluorescent intensity profiles are made by manipulating at least one of: the rates of addition of the fluorescent or optical density material, the stirring rates of the bulk microbead suspension time of exposure of said fluorescent material to the bulk microbead suspension during polymerization or dying of the microbeads.
Claim 3. The Microbead populations of Claim 1, wherein said specific fluorescent intensity profiles are made after the polymerization and dying of the microbeads by combining and mixing selected microbead populations of said selected intensity and distribution width to provide the said fluorescent or optical density profiles matching those of biological cell populations.
Claim 4. The Microbead populations of Claim 1, wherein the specific fluorescent intensity profiles are made by precision photo bleaching portions of microbead populations so that when these portions are combined, provide the said fluorescent intensity profiles matching those of fluorescently labeled biological cell populations.
Claim 5. A method of producing microbeads that provide specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cell populations using the Microbead populations of claim 2.
Claim 6. A method of producing microbeads that provide specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cell populations using the Microbead populations of claim 3.
Claim 7. A method of producing microbeads that provide specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cell populations using the Microbead populations of claim 4.
Claim 8. A method of producing microbeads that provide a specific fluorescence intensity profiles to microbead populations that mimic fluorescently labeled biological cell populations using photobleaching.
Claim 9. The method of Claim 8, wherein the production of said microbeads is achieved through an optical density gradient mask that have been uniformly deposited over a surface.
Claim 10. The method of Claim 8, further enabling the production of microbeads by flowing a suspension of microbeads past a strong source of illumination at various rates.
Claim 11. A method of producing an optical density gradient, used in the method of claim 8, to produce said microbead populations that have optical intensity profiles to microbead populations that mimic said labeled biological cell populations comprising; a. obtaining a list mode file of the intensity profile of the bioloigical cell population of interest from at least a flow cytometer; b. mathematically inverting the number of events in each channel by subtracting the events in each channel from the number of events in the channel with the maximum events; c. converting the number of events to a percentile in each channel; d. sorting the percentiles in an ascending or descending order; e. generating an optical density gradient mask on a transparent material proportional to the sorted percentiles.
Claim 12. An apparatus suitable to photobleach microbead populations so that said population of microbeads mimics the fluorescence intensity profile of said labeled biological cells which is comprised of at least one of: a light source of optimum wavelengths that has uniform intensity across a given area, an optical density gradient mask reflecting the intensity profile of a specific biological cell population and an area of microbeads that have been uniformly deposited so that the illumination of the said light source passes through said density gradient onto the uniformly deposited said microbeads.
PCT/US2009/033519 2009-02-09 2009-02-09 A new generation of fluorescent microbead cellular surrogate standards WO2010090645A1 (en)

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Citations (4)

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US4714682A (en) * 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells
US5084394A (en) * 1984-12-24 1992-01-28 Vogt Robert F Method for corrective calibration of a flow cytometry using a mixture of fluorescent microbeads and cells
US5380663A (en) * 1984-12-24 1995-01-10 Caribbean Microparticles Corporation Automated system for performance analysis and fluorescence quantitation of samples

Patent Citations (4)

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Publication number Priority date Publication date Assignee Title
US3631772A (en) * 1969-07-09 1972-01-04 Bell Telephone Labor Inc Method and apparatus for characterizing photoresist
US5084394A (en) * 1984-12-24 1992-01-28 Vogt Robert F Method for corrective calibration of a flow cytometry using a mixture of fluorescent microbeads and cells
US5380663A (en) * 1984-12-24 1995-01-10 Caribbean Microparticles Corporation Automated system for performance analysis and fluorescence quantitation of samples
US4714682A (en) * 1985-12-11 1987-12-22 Flow Cytometry Standards Corporation Fluorescent calibration microbeads simulating stained cells

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Title
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