Methods of recombining nucleic acids are provided. In particular, methods for the production of partially double stranded nucleic acids comprising a 3′ overhang from an RNA target and use in methods of recombining polynucleotides is described. These methods do not require thermocycling. The present...http://www.google.de/patents/US7176025?utm_source=gb-gplus-sharePatent US7176025 - Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination
