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US006936443B2

(12) United States Patent (io) Patent No.: US 6,936,443 B2

Cohenford et al. (45) Date of Patent: Aug. 30,2005 Page 2

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"Detection of E6 Gene Region DNA of High-Risk Human Papillomavirus Types Using E6 Gene Region Primers" Biosearch International, Aug. 2000, pp. 1-22. Behn M. et al., "Simple and Reliable Factor V Genotyping by PNA-Mediated PCR Clamping" Thromb Haemost. vol. 79, 1998, pp. 773-777.

Cochet O. et al., "Selective PCR Amplification of Functional Immunoglobulin Light Chain from Hybridoma Containing the Aberrant MOPC 21-Derived VX by PNA-Mediated PCR Clamping" Biotechniques. vol. 26, No. 5, 1999, 818-822.

Demers D. B. et al., "Enhanced PCR Amplification of VNTR Locus DIS80 Using Peptide Nucleic Acid (PNA)" Nucleic Acids Research, vol. 23, No. 15, 1995, pp 3050-3055.

Hansen M. H. et al., "Detection of PNA/DNA Hybrid Molecules by Antibody Fab Fragments Isolated from a Phage Display Library" Journal of Immunological Methods. vol. 203, No. 2, 1997, pp. 199-207.

Kyger E. M. et al., "Detection of the Hereditary Hemochromatosis Gene Mutation by Real-Time Fluorescence Polymerase Chain Reaction and Peptide Nucleic Acid Clamping" Analytical Biochemistry, vol. 260, No. 2,1998, pp 142-148. Mrozikiewicz O.M. et al., "Peptide Nucleic Acid-Mediated Polymerase Chain Reaction Clamping Allows Allelic Allocation of CYPIA1 Mutations" Analytical Biochemistry. vol. 250, No. 2, 1997, pp. 256-257.

(Continued)

Primary Examiner—-Jeffrey Fredman

Assistant Examiner—-Teresa Strzelecka

(74) Attorney, Agent, or Firm—-Theodore Allen; Mark J.

Casey

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OTHER PUBLICATIONS

Oram H. et al., "Single Base Pair Mutation Analysis by PNA Direct PCT Clamping" Nucleic Acids Research. Vo 21, No. 23, 1993, pp 5332-5336.

Seeger et al. "PNA-Mediated Purification of PCR Amplifiable Human Genome DNA from Whole Blood" Biotechniques. vol. 23, No. 3, 1997, pp. 312-317. Uhlmann R, "Peptide Nucleic Acids (PNA) and PNA-DNA Chimerase From High Binding Affinity Towards Biological Function" Biol. Chemistry, 1998, 379 (8-9) pp. 1044-1052.

von Wintzingerods F. et al., "Peptide Nucleic Acid-Mediated PCR Clamping as a Useful Supplement in the Determination of Microbial Diversity" Applied and Environmental Microbiology, vol. 66, No. 2, Feb. 2000, pp. 549-557.

Zhong S et al., "Detection of Apoliporotein B mRNA Editing by Peptide Nucleic Acid Mediated PCR Clamping" Biochemical and Biophysical Research Communication, vol. 259, No. 2, 1999, pp. 311-313.

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