United States Patent im
Gross et al.
[in 3,990,851 [45] Nov. 9, 1976
[54] PROCESS AND DEVICE FOR MEASURING ANTIGEN-ANTIBODY REACTIONS
[75] Inventors: Jurgen Gross, Hofheim, Taiinus;
Arno Hoist; Helmut Lask, both of
Wiesbaden; Axel Sieber,
Marburg-Marbach, all of Germany
[73] Assignee: Behringwerke Aktiengesellschaft,
Marburg an der Lahn, Germany
[22] Filed: Feb. 25, 1975
[21] Appl. No.: 552,883
[30] Foreign Application Priority Data
Feb. 27, 1974 Germany 2409273
[52] U.S. CI 23/253 R; 23/230 B;
250/574; 356/103; 356/208; 424/12
[51 ] Int. CI.2 G01N 33/16; G01N 21/24
[58] Field of Search 23/230 B, 253 R;
250/574, 575; 356/103, 208; 424/12
[56] References Cited
UNITED STATES PATENTS
3,646,352 2/1972 Bol et al 356/103 X
3,786,261 1/1974 Tucker. 250/574 X
3,830,569 8/1974 Meric 356/102 X
3,835,315 9/1974 Gravitt, Jr 250/574
3,843,268 10/1974 Kaye 250/574
Primary Examiner—R.E. Serwin
Attorney, Agent, or Firm—Curtis, Morris & Safford
[57] ABSTRACT
Antigen-antibody reactions are measured by passing laser light through the mixture of the antigen solution with the corresponding antibody and measuring the light scattered in the forward direction and determining its intensity. The device used comprises a measuring system consisting of a laser, at least two diaphragms, a system of lenses and a photodetector and a light trap having a diameter corresponding to 1.1 to 1.7 times the diameter of the laser beam.
4 Claims, 3 Drawing Figures
