Humanized antibody and uses thereof

(54) HUMANIZED ANTIBODY AND USES THEREOF

(75) Inventors: Wayne A. Marasco, Wellesley, MA (US); Joyce Lavecchio, Charlestown, MA (US); Abner Mhashilkar, Houston, TX (US); Urban Ramstedt, Boston, MA (US); David Ring, Palo Alto, CA (US); Bridget Eberhardt, Rowley, MA (US); Julie Porter-Brooks, Westford, MA (US)

(73) Assignees: Dana-Farber Cancer Institute, Inc., Boston, MA (US); Avant Immunotherapeutics, Inc., Needham, MA (US); Chiron Corporation,

Emeryville, CA (US)

( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days.

(21) Appl. No.: 09/660,169

(22) Filed: Sep. 12, 2000

Related U.S. Application Data

(63) Continuation of application No. PCT/US99/05262, filed on Mar. 11, 1999

(60) Provisional application No. 60/077,845, filed on Mar. 13, 1998.

(51) Int. CI.7 C12N 5/06; G01N 33/53;

C12P 21/04; C07K 16/00

(52) U.S. CI 435/339.1; 435/7.1; 435/69.7;

530/388.35

(58) Field of Search 530/388.35; 435/7.1,

435/69.7, 339.1

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Primary Examiner—Hankyel T. Park

(74) Attorney, Agent, or Firm—Nixon Peabody LLP

(57) ABSTRACT

A humanized antibody framework motif is described. Preferably, the motif is encoded by the V^ gene of K5B8 and the WL gene of TR1.6. This humanized antibody preferably contains the variable region of a tat antibody.

32 Claims, 13 Drawing Sheets

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