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(54) METHOD FOR CREATING PERFUSABLE MICROVESSEL SYSTEMS
(75) Inventor: Thomas Neumann, Seattle, WA (U S)
(73) Assignee: Nortis, Inc., Gig Harbor, WA (U S)
( * ) Notice: Subject to any disclaimer, the term of tl1is
patent is extended or adjusted under 35 U.S.C. 154(b) by 916 days.
This patent is subject to a tenninal disclaimer.
(21) Appl.No.: 11/860,471 (22) Filed: Sep.24,2007
(65) Prior Publication Data US 2008/0261306 A1 Oct. 23, 2008
Related U.S. Application Data
(63) Continuation-in-part of application No. 11/388,920, filed on Mar. 24, 2006, now Pat. No. 7,622,298.
(51) Int. Cl. C12N 5/00 (2006.01) (52) U.S. Cl. ....................... .. 435/395; 435/325; 435/397 (58) Field of Classification Search ................ .. 435/395, 435/325, 397 See application file for complete search history. (56) References Cited U.S. PATENT DOCUMENTS 4,878,908 A 11/1989 Martin et al. 5,804,366 A 9/1998 Hu et al. 6,503,273 B1 1/2003 McAllister et al 6,592,623 B1 7/2003 Bowin et al. 6,642,019 B1 11/2003 Anderson et al. 6,893,812 B2 5/2005 Woltering 6,989,071 B2 1/2006 Kocur et al. 2002/0150879 A1 10/2002 Woltering et al. 2002/0177121 A1 11/2002 Woltering et al. 2003/0138945 A1 7/2003 McAllister et al 2003/0138950 A1 7/2003 McAllister et al. 2003/0171053 A1 9/2003 Sanders 2004/0044403 A1 3/2004 Bischoff et al. 2006/0216320 A1 9/2006 Kitazono et al. 2007/0110962 A1 5/2007 Tien et al. 2007/0224677 A1 9/2007 Neumann OTHER PUBLICATIONS
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Primary Examiner — Ruth Davis (74) Attorney, Agent, or Firm — George A. Leone; Citadel Patent Law
A method for creating networks of perfusable microves sels in vitro. A mandrel is drawn through a matrix to fonn a channel through the matrix. Cells are injected into the channel. The matrix is incubated to allow the cells to attach inside the channel. The channel is perfused to remove unattached cells to create a parent vessel, where the parent vessel includes a perfusable hollow channel lined with cells in the matrix. The parent vessel is induced to create sprouts into the surrounding matrix gel so as to form a microvessel network. The microvessel network is subjected to luminal perfusion through the parent vessel.
12 Claims, 10 Drawing Sheets
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