[54] REACTION CHAMBER FOR
AMINO-TERMINAL SEQUENCE ANALYSIS
OF PROTEINS OR PEPTIDES
[75] Inventor: Toyoaki Uchida, Tokyo, Japan
[73] Assignee: Seiko Instruments Inc., Japan
[21] Appl. No.: 530,203
[22] Filed: May 30,1990
[51] Int. CI.' B03C 1/02; C12N 13/00;
C12M 1/34; G01N 33/553
[52] u.s. ci :: 422/107; 422/116;
422/69; 436/526; 435/291; 435/173; 435/180;
435/287; 210/222
[58] Field of Search 422/107, 186.01, 106,
422/108, 70, 116, 69; 210/222; 436/526;
435/287, 291, 173, 180
[30] Foreign Application Priority Data
May 23, 1989 [JP] Japan 1-129740
[56] References Cited
U.S. PATENT DOCUMENTS
4,141,687 2/1979 Forrest et al 210/222
4,181,853 1/1980 Abu-Shumays et al 422/69
4,375,407 3/1983 Kronick 436/526
4,649,116 3/1987 Daly et al 435/287
4,695,393 9/1987 Whitehead et al 436/526
4,710,472 12/1987 Saur et al 435/287
4,855,045 8/1989 Reed 210/222
4,865,730 9/1989 Lam et al 210/222
Primary Examiner—Robert J. Warden
Assistant Examiner—T. A. Trembley
Attorney, Agent, or Firm—Bruce L. Adams; Van C.
Wilks
[57] ABSTRACT
A reaction chamber utilizes a sample carrier composed of a magnetic core and a surface coating effective to support sample of protein or peptide. The sample carrier is floated magnetically by means of electromagnets positioned within a reaction vessel. Edman reagnet is applied to the sample to effect amino acid sequence analysis of protein or peptide from amino-terminal. By such construction, reaction efficiency of repeated production of thiazolinon amino-acid derivatives is increased so as to increase number of identified amino acids, thereby enabling microanalysis of sample.
