Abstract
Dragon’s head (Lallemantia iberica) is a medicinal and oilseed crop cultivated in different parts of Iran. In Jun 2018 in the experimental plots in Gonbad-Kavous, and 2022 in a commercial field in Gorgan, both located in the north of country, a typical Sclerotinia stem rot was observed. The isolated Sclerotinia-like fungus from the infected plant sample was subjected to morphological as well as molecular and phylogenetic investigations using the internal transcribed spacer gene region. The obtained results showed that the fungus is Sclerotinia sclerotiorum. The pathogenicity tests on the plant in the field and under controlled conditions were positive. Koch’s postulates were confirmed through the successful re-isolation of the pathogen. To the extent of our knowledge, this is the first report of the disease not only in Iran but also in the world.
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References
Abdolahi M, Maleki Farahani S (2019) Seed quality, water use efficiency and eco physiological characteristics of Lallemantia (Lallemantia sp.) species as effected by soil moisture content. Acta Agric Slov 113:307–320. https://doi.org/10.14720/aas.2019.113.2.12
Al-Snafi AE (2019) Medical benefit of Lallemantia Iberica: a review. To Chem J 3:97–102
Amanzadeh Y, Khosravi Dehaghi N, Gohari AR et al (2011) Antioxidant activity of essential oil of Lallemantia iberica in flowering stage and post-flowering stage. Res J Biol Sci 6:114–117
Asghari G, Akbari M, Asadi-Samani M (2017) Phytochemical analysis of some plants from Lamiaceae family frequently used in folk medicine in Aligudarz region of Lorestan province. Marmara Pharm J 21:506–514. https://doi.org/10.12991/marupj.311815
Baral HO, Rönsch P, Richter U et al (2022) Schroeteria decaisneana, S. poeltii, and Ciboria ploettneriana (Sclerotiniaceae, Helotiales, Ascomycota), three parasites on Veronica seeds: first report of teleomorphs in Schroeteria. Mycol Prog 21:359–407. https://doi.org/10.1007/s11557-021-01742-4
Baturo-Ciesniewska A, Andrzejewska J, Albrecht KA et al (2013) First report of sclerotinia stem blight caused by Sclerotinia trifoliorum on Trifolium ambiguum in Poland. Plant Dis 97:142. https://doi.org/10.1094/PDIS-07-12-0624-PDN
Baturo-Ciesniewska A, Groves CL, Albrecht KA et al (2017) Molecular identification of Sclerotinia trifoliorum and Sclerotinia sclerotiorum isolates from the United States and Poland. Plant Dis 101:192–199. https://doi.org/10.1094/PDIS-06-16-0896-RE
Boland GJ, Hall R (1994) Index of plant hosts of Sclerotinia sclerotiorum. Can J Plant Pathol 16:93–108. https://doi.org/10.1080/07060669409500766
Brodal G, Warmington R, Grieu C et al (2017) First report of Sclerotinia subarctica nom. Prov. (Sclerotinia sp.) causing stem rot on turnip rape (Brassica rapa subsp. oleifera) in Norway. Plant Dis 101:138–147. https://doi.org/10.1094/PDIS-06-16-0785-PDN
Dellaporta CSL, Wood J, Hicks JB, Dna A (1983) A plant DNA minipreparation: version II. Plant Mol Biol Report 1:19–21
Farr DF, Rossman AY (2020) Fungal databases, U.S. National fungus collections, ARS, USDA. In: fungal databases, U.S. Natl. Fungus Collect. ARS, USDA.
Heydari S, Pirzad A (2021) Efficiency of Funneliformis mosseae and Thiobacillus sp. on the secondary metabolites (essential oil, seed oil and mucilage) of Lallemantia iberica under salinity stress. J Hortic Sci Biotechnol 96:249–259. https://doi.org/10.1080/14620316.2020.1833764
Hoffman DD, Diers BW, Hartman GL et al (2002) Selected soybean plant introductions with partial resistance to Sclerotinia sclerotiorum. Plant Dis 86:971–980. https://doi.org/10.1094/PDIS.2002.86.9.971
Holst-Jensen A, Vaage M, Schumacher T (1998) An approximation to the phylogeny of Sclerotinia and related genera. Nord J Bot 18:705–719. https://doi.org/10.1111/j.1756-1051.1998.tb01553.x
Hu J, Zhou Y, Geng J et al (2019) A new dollar spot disease of turfgrass caused by Clarireedia paspali. Mycol Prog 18(12):1423–1435. https://doi.org/10.1007/s11557-019-01526-x
Jones G, Valamoti SM (2005) Lallemantia, an imported or introduced oil plant in Bronze Age northern Greece. Veg Hist Archaeobot 14:571–577. https://doi.org/10.1007/s00334-005-0004-z
Kohn LM (1979) Delimitation of the economically important plant pathogenic Sclerotinia species. Phytopathology 69:881–886. https://doi.org/10.1094/phyto-69-881
Koike ST, Stanghellini H, Mauzey SJ, Burkhardt A (2019) First Report of Sclerotinia crown rot caused by Sclerotinia minor on Hemp. Plant Dis 103(7):1771. https://doi.org/10.1094/PDIS-01-19-0088-PDNIn
Komartin RS, Stroescu M, Chira N et al (2021) Optimization of oil extraction from Lallemantia iberica seeds using ultrasound-assisted extraction. J Food Meas Charact 15:2010–2020. https://doi.org/10.1007/s11694-020-00790-w
Li CX, Li H, Siddique AB et al (2007) The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B. juncea to Sclerotinia sclerotiorum. Aust J Agric Res 58:1198–1203. https://doi.org/10.1071/AR07094
Mahmood S, Hayat MQ, Sadiq A et al (2013) Antibacterial activity of Lallemantia royleana (Benth.) indigenous to Pakistan. African J Microbiol Res 7:4006–4009. https://doi.org/10.5897/ajmr2013.5845
Marin MV, Peres NA (2020) First report of sclerotinia sclerotiorum causing strawberry fruit rot in Florida. Plant Dis 104:3250. https://doi.org/10.1094/PDIS-04-20-0879-PDN
Morteza-Semnani K (2006) Essential oil composition of lallemantia iberica fisch. et C.A. Mey J Essent Oil Res 18:164–165. https://doi.org/10.1080/10412905.2006.9699055
Naghibi F, Mosaddegh M, Motamed SM (2005) Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iran J Pharm Res IJPR 4:63–79. https://doi.org/10.22037/ijpr.2010.619
Nikitina AS, Popova OI, Ushakova LS et al (2008) Studies of the essential oil of Dracocephalum moldavica cultivated in the Stavropol region. Pharm Chem J 42:203–207. https://doi.org/10.1007/s11094-008-0092-z
Nori-Shargh D, Kiaei SM, Deyhimi F et al (2009) The volatile constituents analysis of Lallemantia iberica (M.B.) Fischer & Meyer from Iran. Nat Prod Res 23:546–548. https://doi.org/10.1080/14786410601132394
Ozdemir FA, Yildirim MU, Pourali Kahriz M (2014) Efficient Micropropagation of Highly Economic, Medicinal and Ornamental Plant Lallemantia iberica (Bieb.) Fisch and CA Mey. Biomed Res Int 476346:5. https://doi.org/10.1155/2014/476346
Sadeghi-Varkani A, Emam-Djomeh Z, Askari G (2018) Morphology and physicochemical properties of a novel Lallemantia iberica mucilage/titanium dioxide bio-nanocomposite. Polym Test 67:12–21. https://doi.org/10.1016/j.polymertesting.2018.02.006
Samadi S, Khaiyamiand M, Hasanzadeh Gourt Tappe A (2007) A comparison of important physical and chemical characteristics of six Lallemant iaiberica (Bieb.) Fisch and Mey varieties. Pakistan J Nutr 6:387–390
Shafiee S, Motlagh AM, Minaee S, Haidarbigi K (2009) Moisture-dependent physical properties of Dragon’s head seeds (Lallemantia iberica). Agric Eng Int the CIGR J XI:Manuscript 1192
Sharma P, Meena PD, Verma PR et al (2016) Sclerotinia sclerotiorum (Lib) de Bary causing Sclerotinia rot in oilseed Brassicas: a review. J Oilseed Brassica 1:1–44
Singh SK, Srivastava HP (1988) Symptoms of Macrophomina phaseolina infections on moth bean seedlings. Ann Arid Zone 27:151–152
Taghinia P, Abdolshahi A, Sedaghati S, Shokrollahi B (2021) Smart edible films based on mucilage of Lallemantia iberica seed incorporated with curcumin for freshness monitoring. Food Sci Nutr 9:1222–1231. https://doi.org/10.1002/fsn3.2114
Tamura K, Stecher G, Kumar S (2021) MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027. https://doi.org/10.1093/molbev/msab120
Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680. https://doi.org/10.1093/nar/22.22.4673
Usher G (1974) A dictionary of plants used by man. 619 Seiten. Constable and Company Ltd., London 1974. London, UK
Uzuhashi S, Ohtaka N, Hirooka Y et al (2010) Dumontinia root rot of liver leaf caused by Dumontinia tuberosa. J Gen Plant Pathol 76:705–719. https://doi.org/10.1007/s10327-010-0235-4
Vu D, Groenewald M, de Vries M et al (2019) Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher taxon delimitation. Stud Mycol 92:135–154. https://doi.org/10.1016/j.simyco.2018.05.001
White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA Genes for phylogenetics. In: Gelfand DH, Sinsky JT, White T (eds) Innis MA. A Guide to Methods and Applications. Academic Press, PCR Protocols, pp 315–322
Zhao J, Peltier AJ, Meng J et al (2004) Evaluation of sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Dis 88:1033–1039. https://doi.org/10.1094/PDIS.2004.88.9.1033
Zlatanov M, Antova G, Angelova-Romova M et al (2012) Lipid structure of Lallemantia seed oil: a potential source of omega-3 and omega-6 fatty acids for nutritional supplements. JAOCS, J Am Oil Chem Soc 89:1393–1401. https://doi.org/10.1007/s11746-012-2042-x
Acknowledgements
This study was funded by DARI, AREEO through project number 2-15-15-022-961237. The authors would like to thank Dr. B. Asghari Khosrowshahi and P. Mohammadian for technical support.
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The fungus is a plant pathogen. So, no studies with human participants or animals were performed by any of the authors.
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Pouralibaba, H.R., Kheirgoo, M., Kowkab, S. et al. Sclerotinia sclerotiorum causes Sclerotinia stem rot on Dragon's head (Lallemantia iberica). J Plant Dis Prot 130, 431–436 (2023). https://doi.org/10.1007/s41348-023-00704-0
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DOI: https://doi.org/10.1007/s41348-023-00704-0