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Pathotyping of white rust pathogen Albugo candida on Brassicaceae hosts in India

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Abstract

The white rust caused by Oomycetes Albugo candida (Pers.) Kuntze infects cruciferous crops and results in huge economic loss at a global level. To cope with this problem, identification of a resistance source against morphologically and pathologically diverse white rust is one of the necessary objectives to improve the cultivar’s yield. In this study, eighteen diverse Brassica genotypes including one susceptible variety Varuna were evaluated against twelve (A) candida isolates collected from the different geographical locations in Indian. Based on the PCA analysis, the morphological characters loaded, were found to contribute 58.6% and 38.3% of total variability through pustule size and sporangia size, respectively. Whereas latent period only contributes 3% of total variability among WRI. While comparing Brassicaceae hosts, no disease symptoms was observed on two Brassica spp. viz. (B) napus cv. GSL-1 and B. carinata cv. DLSC-1 that consistently showed complete resistance against all WR isolates. Also, the two DH B. juncea mutant lines C66 and C69 showed a promising level of resistance against A. candida where C66 was found completely resistant against 09 white rust isolates (WRI) except susceptible to WRI-K isolate while, C69 was found to be second best in terms of resistance against WRI-WB isolate followed by 08 other isolates except WRI-K, WRI-M and WRI-S. With changing habitats and unavailability of a significant solution, these findings would help in identifying genetic resistance against a widened horizon enhancing the scope of deploying white rust resistance in the susceptible Indian mustard genotypes.

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Acknowledgements

We are grateful to Dr. Ginette Seguin-Swartz, former scientist, Agriculture and Agri-Food Canada Research Station, Saskatoon, Canada and Prof. S.J. Kolte, former professor (plant pathology), G. B. Pant University of Agriculture and Technology, Pantnagar, India for manuscript correction and constructive suggestions to finalise the manuscript. We are thankful to Dr Niraj Kumar Singh, Amity Institute of Applied Science, Amity University and Dr Rajeev Nayan Bahuguna, Dr Rajendra Prasad Central Agriculture University Pusa, Bihar for helping in statistical analysis at revision stage. We also thank Prof. Ajit Varma, Head of the Department, AIMT, Amity University, Noida for providing research facilities and Ms. Surbhi Dabral, Technical Officer, AIMT for helping in confocal microscopy.

Funding

The funding was from Department of Biotechnology (DBT), Government of India under project sanctioned, BT/IN/Indo-UK/CGAT/12/DP/2014-2015 dated 8th May 2015; PI/ Co-PI- Prof Abha Agnihotri & Prof Pankaj Sharma; JRF/ SRF- Anupriya Chatterjee & Shradha Nirwan.

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Authors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, 201303, India

    Shradha Nirwan, Anupriya Chatterjee, Abha Agnihotri & Neeraj Shrivastava

  2. Department of Plant Pathology, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India

    Anand Kumar Tewari

  3. Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, 201303, India

    Pankaj Sharma

  4. Department of Biotechnology, Jamia University Hamdard, Hamdard Nagar, New Delhi, Delhi, India

    Malik Zainul Abdin

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  1. Shradha Nirwan
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  2. Anupriya Chatterjee
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  3. Anand Kumar Tewari
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  4. Abha Agnihotri
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  7. Neeraj Shrivastava
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Contributions

Experimental design: AA and PS. Pathological study design: AKT. Isolate collection: AKT and SN. Morphological and pathological assessments and collection of data: SN and AC. Manuscript draft preparation: SN and NS. Figure concept and design: NS and SN, Manuscript correction and language edition: NS, AA, and MZA. All authors reviewed the manuscript.

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Correspondence to Anand Kumar Tewari, Abha Agnihotri or Neeraj Shrivastava.

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Nirwan, S., Chatterjee, A., Tewari, A.K. et al. Pathotyping of white rust pathogen Albugo candida on Brassicaceae hosts in India. Indian Phytopathology 75, 383–393 (2022). https://doi.org/10.1007/s42360-022-00473-0

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