CN104471075A - 核酸扩增 - Google Patents
核酸扩增 Download PDFInfo
- Publication number
- CN104471075A CN104471075A CN201380031868.1A CN201380031868A CN104471075A CN 104471075 A CN104471075 A CN 104471075A CN 201380031868 A CN201380031868 A CN 201380031868A CN 104471075 A CN104471075 A CN 104471075A
- Authority
- CN
- China
- Prior art keywords
- primer
- optionally
- nucleic acid
- amplification
- template
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 0 *NC(c1cc(S)ccc1)=O Chemical compound *NC(c1cc(S)ccc1)=O 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810576963.4A CN109486902B (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202310142485.7A CN116064734A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202210533890.7A CN114854832A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261635584P | 2012-04-19 | 2012-04-19 | |
| US61/635,584 | 2012-04-19 | ||
| US201261699810P | 2012-09-11 | 2012-09-11 | |
| US61/699,810 | 2012-09-11 | ||
| US201361767766P | 2013-02-21 | 2013-02-21 | |
| US61/767,766 | 2013-02-21 | ||
| US201361781016P | 2013-03-14 | 2013-03-14 | |
| US61/781,016 | 2013-03-14 | ||
| US201361792247P | 2013-03-15 | 2013-03-15 | |
| PCT/US2013/032598 WO2013158313A1 (en) | 2012-04-19 | 2013-03-15 | Nucleic acid amplification |
| US61/792,247 | 2013-03-15 |
Related Child Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310142485.7A Division CN116064734A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN201810576963.4A Division CN109486902B (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202210533890.7A Division CN114854832A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104471075A true CN104471075A (zh) | 2015-03-25 |
| CN104471075B CN104471075B (zh) | 2018-06-22 |
Family
ID=48045103
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380031868.1A Active CN104471075B (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202310142485.7A Pending CN116064734A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202210533890.7A Pending CN114854832A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN201810576963.4A Active CN109486902B (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310142485.7A Pending CN116064734A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN202210533890.7A Pending CN114854832A (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
| CN201810576963.4A Active CN109486902B (zh) | 2012-04-19 | 2013-03-15 | 核酸扩增 |
Country Status (3)
| Country | Link |
|---|---|
| CN (4) | CN104471075B (de) |
| SG (2) | SG11201406717RA (de) |
| WO (1) | WO2013158313A1 (de) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107849598A (zh) * | 2015-05-29 | 2018-03-27 | 伊卢米纳剑桥有限公司 | 簇中的表面引物的增强利用 |
| CN108291251A (zh) * | 2015-09-22 | 2018-07-17 | 生命技术公司 | 用于核酸分析的系统和方法 |
| CN108368544A (zh) * | 2015-09-29 | 2018-08-03 | 生命技术公司 | 用于执行数字pcr的系统和方法 |
| CN106370831B (zh) * | 2016-08-29 | 2019-06-14 | 苏州奥普特克自动化科技有限公司 | 用于生物分子相互作用动态检测的检测芯片及制备方法 |
| CN110249059A (zh) * | 2017-01-20 | 2019-09-17 | 欧姆尼欧美公司 | 核酸的等位基因特异性捕获 |
| US10975424B2 (en) | 2017-01-20 | 2021-04-13 | Omniome, Inc. | Genotyping by polymerase binding |
| CN113272449A (zh) * | 2018-10-26 | 2021-08-17 | Illumina公司 | 调整聚合物小珠以进行dna处理 |
| CN113906148A (zh) * | 2019-05-03 | 2022-01-07 | 生命技术公司 | 用于操纵核酸的方法和组合物 |
| CN113943788A (zh) * | 2018-11-14 | 2022-01-18 | 元素生物科学公司 | 用于改进的固相dna杂交和扩增的低结合载体 |
| CN109862965B (zh) * | 2016-10-21 | 2022-05-03 | Gna生物解决办法有限公司 | 用于进行聚合酶链式反应的方法和用于进行该方法的装置 |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| EP2652148B1 (de) | 2010-12-17 | 2016-11-30 | Life Technologies Corporation | Verfahren, zusammensetzungen, systeme, vorrichtungen und kits für nukleinsäureamplifikation |
| EP3763818A1 (de) | 2015-10-06 | 2021-01-13 | Pierce Biotechnology, Inc. | Vorrichtungen und verfahren zur herstellung von proteinen |
| WO2019094524A1 (en) | 2017-11-07 | 2019-05-16 | Life Technologies Corporation | Methods and compositions for manipulating nucleic acids |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| US11905558B2 (en) | 2019-08-21 | 2024-02-20 | Life Technologies Corporation | System and method for sequencing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007107710A1 (en) * | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
| WO2013045700A1 (de) * | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen anordnung von probenfragmenten zur amplifikation und immobilisierung für weitere derivatisierungen |
Family Cites Families (36)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| IL86724A (en) | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| DK0612352T3 (da) | 1990-05-07 | 1997-06-30 | Daikin Ind Ltd | Diagnostiske anvendelser af dobbelt-D-sløjfedannelse |
| US5273881A (en) | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
| US5194370A (en) | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
| EP1275735A1 (de) * | 2001-07-11 | 2003-01-15 | Roche Diagnostics GmbH | Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| AU2003215391B2 (en) | 2002-02-21 | 2007-05-24 | Abbott Diagnostics Scarborough, Inc. | Recombinase Polymerase Amplification |
| EP2159285B1 (de) | 2003-01-29 | 2012-09-26 | 454 Life Sciences Corporation | Verfahren zur Verstärkung und Sequenzierung von Nukleinsäuren |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| WO2006084131A2 (en) | 2005-02-01 | 2006-08-10 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Furin inhibitors and alpha-defensins for the treatment or prevention of papillomavirus infection |
| EP1910537A1 (de) | 2005-06-06 | 2008-04-16 | 454 Life Sciences Corporation | Sequenzierung mit gepaarten enden |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| CA2650993C (en) | 2006-05-04 | 2015-06-16 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| KR20080029233A (ko) * | 2006-09-28 | 2008-04-03 | 삼성전자주식회사 | 핵산 증폭 및 혼성화를 단일 고체 지지체에서 수행하는방법 및 장치 |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP2653861B1 (de) | 2006-12-14 | 2014-08-13 | Life Technologies Corporation | Verfahren zur Nukleinsäure-Sequenzierung mittels großer FET-Arrays |
| US7932034B2 (en) | 2006-12-20 | 2011-04-26 | The Board Of Trustees Of The Leland Stanford Junior University | Heat and pH measurement for sequencing of DNA |
| GB2461127B (en) | 2008-06-25 | 2010-07-14 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale FET arrays |
| US20100301398A1 (en) | 2009-05-29 | 2010-12-02 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US8546128B2 (en) | 2008-10-22 | 2013-10-01 | Life Technologies Corporation | Fluidics system for sequential delivery of reagents |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
| WO2011106629A2 (en) | 2010-02-26 | 2011-09-01 | Life Technologies Corporation | Modified proteins and methods of making and using same |
| EP2652148B1 (de) | 2010-12-17 | 2016-11-30 | Life Technologies Corporation | Verfahren, zusammensetzungen, systeme, vorrichtungen und kits für nukleinsäureamplifikation |
| LT2742151T (lt) | 2011-08-10 | 2018-02-12 | Life Technologies Corporation | Polimerazės kompozicija |
-
2013
- 2013-03-15 WO PCT/US2013/032598 patent/WO2013158313A1/en active Application Filing
- 2013-03-15 CN CN201380031868.1A patent/CN104471075B/zh active Active
- 2013-03-15 SG SG11201406717RA patent/SG11201406717RA/en unknown
- 2013-03-15 CN CN202310142485.7A patent/CN116064734A/zh active Pending
- 2013-03-15 CN CN202210533890.7A patent/CN114854832A/zh active Pending
- 2013-03-15 CN CN201810576963.4A patent/CN109486902B/zh active Active
- 2013-03-15 SG SG10201802883UA patent/SG10201802883UA/en unknown
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007107710A1 (en) * | 2006-03-17 | 2007-09-27 | Solexa Limited | Isothermal methods for creating clonal single molecule arrays |
| WO2013045700A1 (de) * | 2011-09-30 | 2013-04-04 | Albert-Ludwigs-Universität Freiburg | Verfahren zur räumlichen anordnung von probenfragmenten zur amplifikation und immobilisierung für weitere derivatisierungen |
Non-Patent Citations (1)
| Title |
|---|
| TOM MORRISON 等: "nanoliter high throughput quantitative PCR", 《NUCLEIC ACIDS RESEARCH》, 25 September 2006 (2006-09-25) * |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107849598B (zh) * | 2015-05-29 | 2021-12-14 | 伊卢米纳剑桥有限公司 | 簇中的表面引物的增强利用 |
| CN107849598A (zh) * | 2015-05-29 | 2018-03-27 | 伊卢米纳剑桥有限公司 | 簇中的表面引物的增强利用 |
| CN108291251A (zh) * | 2015-09-22 | 2018-07-17 | 生命技术公司 | 用于核酸分析的系统和方法 |
| CN108368544A (zh) * | 2015-09-29 | 2018-08-03 | 生命技术公司 | 用于执行数字pcr的系统和方法 |
| CN108368544B (zh) * | 2015-09-29 | 2023-06-23 | 生命技术公司 | 用于执行数字pcr的系统和方法 |
| CN106370831B (zh) * | 2016-08-29 | 2019-06-14 | 苏州奥普特克自动化科技有限公司 | 用于生物分子相互作用动态检测的检测芯片及制备方法 |
| CN109862965B (zh) * | 2016-10-21 | 2022-05-03 | Gna生物解决办法有限公司 | 用于进行聚合酶链式反应的方法和用于进行该方法的装置 |
| CN110249059B (zh) * | 2017-01-20 | 2021-04-13 | 欧姆尼欧美公司 | 核酸的等位基因特异性捕获 |
| US11180749B2 (en) | 2017-01-20 | 2021-11-23 | Omniome, Inc. | Allele-specific capture of nucleic acids |
| US10975424B2 (en) | 2017-01-20 | 2021-04-13 | Omniome, Inc. | Genotyping by polymerase binding |
| CN110249059A (zh) * | 2017-01-20 | 2019-09-17 | 欧姆尼欧美公司 | 核酸的等位基因特异性捕获 |
| CN113272449A (zh) * | 2018-10-26 | 2021-08-17 | Illumina公司 | 调整聚合物小珠以进行dna处理 |
| CN113272449B (zh) * | 2018-10-26 | 2024-03-12 | Illumina公司 | 调整聚合物小珠以进行dna处理 |
| CN113943788A (zh) * | 2018-11-14 | 2022-01-18 | 元素生物科学公司 | 用于改进的固相dna杂交和扩增的低结合载体 |
| CN113906148A (zh) * | 2019-05-03 | 2022-01-07 | 生命技术公司 | 用于操纵核酸的方法和组合物 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109486902B (zh) | 2023-02-28 |
| CN109486902A (zh) | 2019-03-19 |
| SG10201802883UA (en) | 2018-05-30 |
| WO2013158313A1 (en) | 2013-10-24 |
| CN104471075B (zh) | 2018-06-22 |
| CN114854832A (zh) | 2022-08-05 |
| CN116064734A (zh) | 2023-05-05 |
| SG11201406717RA (en) | 2014-11-27 |
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Legal Events
| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |