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VeröffentlichungsnummerCN106198598 A
PublikationstypAnmeldung
AnmeldenummerCN 201610536344
Veröffentlichungsdatum7. Dez. 2016
Eingetragen9. Juli 2016
Prioritätsdatum9. Juli 2016
Veröffentlichungsnummer201610536344.3, CN 106198598 A, CN 106198598A, CN 201610536344, CN-A-106198598, CN106198598 A, CN106198598A, CN201610536344, CN201610536344.3
Erfinder倪中华, 易红, 雷鹏坤, 陆荣生, 周新龙
Antragsteller东南大学
Zitat exportierenBiBTeX, EndNote, RefMan
Externe Links:  SIPO, Espacenet
Mini nuclear magnetic resonance (NMR) probe
CN 106198598 A
Zusammenfassung
The invention discloses a mini nuclear magnetic resonance (NMR) probe. The probe comprises a probe body and a detection channel arranged in the probe body. The detection channel comprises a sample inlet, a sample outlet, and a detection cavity, which is arranged between the sample inlet and the sample outlet. The probe is characterized in that the probe body comprises a probe upper layer, a middle layer spacer, and a probe lower layer; a chip groove that can be communicated with the exterior of the probe body is arranged on the middle layer spacer, a carrier chip is arranged in the chip groove, the detection channel is arranged on the carrier chip; the probe upper layer is provided with an upper layer coil, the probe lower layer is provided with a lower layer coil, the upper layer coil and the lower layer coil are electrically connected; the detection cavity is arranged between the upper layer coil and the lower layer coil, and the detection cavity, the upper layer coil, and the lower layer coil are concentric. The provided mini NMR probe can detect a micro-sample, the sample will not be left on the probe, the probe will not be polluted, and thus detection signals, which are more precise and reliable, can be obtained.
Ansprüche(10)  übersetzt aus folgender Sprache: Chinesisch
1.一种微型核磁共振探头,包括探头本体以及置于探头本体内的检测通道,所述检测通道包括样品入口(51 )、样品出口(52)和连接在样品入口与样品出口之间的检测腔(53),其特征在于:所述探头本体包括探头上层(1)、中层垫片(2)、以及探头下层(3),在所述中层垫片上设置有一可与探头本体外部连通的芯片槽,在该芯片槽内设置有一载样芯片(5),所述检测通道设置在所述载样芯片上,在所述探头上层设置有上层线圈,在所述探头下层设置有下层线圈,所述上层线圈与下层线圈电连接,所述检测腔位于所述上层线圈与下层线圈之间且三者同心。 A miniature nuclear magnetic resonance probe comprising a probe body and a probe disposed within the body of the detection channel, the detector includes a sample inlet channel (51), a sample outlet (52) connected between the sample inlet and the sample outlet detecting chamber (53), characterized in that: said upper probe includes a probe body (1), the middle spacer (2), and the lower probe (3), may be provided with a communication with the outside of the probe body in said intermediate pad chip grooves, in which the chip carrier is provided with a groove-like chip (5), said detection channels are provided on the loaded chip, has upper coil disposed in the upper layer of the probe, the probe is provided with a lower lower coil, the upper coil and the lower coil are electrically connected to the detection chamber and three concentrically positioned between the upper coil and lower coil.
2.根据权利要求1所述的微型核磁共振探头,其特征在于:所述探头上层(I)还包含上层基底(11)、上层导线(13)以及上封装层(14),所述上层线圈(12)绕在位于上层基底(11)中部的上层基底柱(111)上,上封装层(14)相邻两边切出上层第一键和口(141)和上层第二键和口(142),所述上层导线(13)设置在第一键和口(141)和上层第二键和口(142)与上层线圈(12)之间;所述中层垫片(2)相邻两边对应上封装层(14)相同位置切出中层第一键和口(21)和中层第二键和口(22),中层垫片(2)中部设置所述芯片槽(23);所述探头下层(3)包含下层基底(31)、下层导线(33)以及下封装层(34),所述下层线圈(32)绕在位于下层基底(31)中部的下层基底柱上(311),下封装层(34)相相邻两边对应上封装层(14)相同位置切出下层第一键和口(341)和下层第二键和口(342),所述上层导线(13)与所述下层导线(33)由第一导体柱(41)和第 The micro NMR probe according to claim 1, wherein: said upper probe (I) further comprises an upper substrate (11), the upper wire (13) and an upper encapsulation layer (14), said upper coil (12) positioned around an upper substrate (11) on the upper substrate column (111) of the middle, upper encapsulation layer (14) adjacent to both sides of the key and cut the upper layer of the first port (141) and an upper opening and a second key (142 ), said upper wire (13) disposed between the first port and the keys (141) and an upper opening and a second key (142) and the upper coil (12); said intermediate pad (2) corresponding to two adjacent sides same position encapsulation layer (14) cut out of the middle of the first key and the opening (21) and middle and a second key opening (22), the middle shim (2) arranged in the middle of the chip groove (23); said lower probe (3) a lower layer comprising a substrate (31), the lower wire (33) and a lower encapsulation layer (34), said lower coil (32) located around the lower substrate (31) underlying substrate column (311) of the middle, lower package layer (34) adjacent to the corresponding sides of the encapsulation layer (14) cut out of the same position as the lower layer and a first key opening (341) and lower keys and the second port (342), said upper wire (13) and the lower conductor (33) from a first conductive post (41) and 二导体柱(42)进行电导通,整体构成亥姆霍兹线圈。 Two conductor post (42) is electrically conductive, the overall configuration of a Helmholtz coil.
3.根据权利要求2所述的微型核磁共振探头,其特征在于:所述下层导线包括下层第一导线(331)和下层第二导线(332),所述下层第一导线(331)连接在第一焊盘(333)与下层第一键和口(341)之间并与所述下层线圈(32)连接,所述下层第二导线(332)连接在第二焊盘(334)与下层第二键和口(342)之间。 3. The micro NMR probe according to claim 2, wherein: the lower layer comprises a lower layer a first lead wire (331) and a second lower conductor (332), the first lower conductor (331) connected a first pad (333) and connected between the lower port and a first key (341) and the lower coil (32), the second lower conductor (332) connected to the second pad (334) and the lower and a second key opening (342) between.
4.根据权利要求2所述的微型核磁共振探头,其特征在于:下封装层(34)覆盖区域不包含第一焊盘(333)与第二焊盘(334)。 According to claim micro NMR probe of claim 2, wherein: the encapsulating layer (34) covering a first region does not include pads (333) and a second pad (334).
5.根据权利要求1-4任一所述的微型核磁共振探头,其特征在于:所述上层基底(11)和下层基底(31)材料为硬质非金属材料;所述上封装层(14)与下封装层(34)材料为光固化材料;所述上层线圈(12)、上层导线(13)、下层线圈(32)和下层导线(33)材料为高导电材料。 5. The micro NMR probe according to claim any one of claims 1-4, characterized in that: said upper base (11) and a lower base (31) of non-metallic material is a hard material; the upper encapsulation layer (14 ) lower encapsulating layer (34) material is a photocurable material; the upper coil (12), the upper wire (13) and lower coil (32) and a lower wire (33) material is a highly conductive material.
6.根据权利要求5所述的微型核磁共振探头,其特征在于:所述硬质非金属材料为玻璃或聚甲基丙烯酸甲酯;所述光固化材料为UV树脂或SU-8;所述高导电材料为铜、银或金。 The micro NMR probe according to claim 5, wherein: said hard non-metallic material is glass or polymethyl methacrylate; the photocurable resin material is a UV or SU-8; the high conductivity material is copper, silver or gold.
7.根据权利要求1-4任一所述的微型核磁共振探头,其特征在于:所述探头上层(I )、中层垫片(2)、探头下层(3)相邻两层间结合方式为不可逆的等离子体氧化粘结或高分子材料粘结。 The micro NMR probe according to any one of claims 1 to 4, wherein: said upper probe (the I), the middle spacer (2), the probe lower layer (3) between adjacent layers for the binding mode plasma oxidation irreversible adhesive bonding or polymer material.
8.根据权利要求3所述的微型核磁共振探头,其特征在于:所述上层导线(13)与下层导线(33)通过电镀铺到上层基底(11)与下层基底(31)上,上层导线(13)分别从上层线圈(12)两端引致相邻上层基底(11)两边;下层第一导线(331)分别从下层线圈(32)两端引致下层第一键和口(341)和第一焊盘(333),下层第二导线(332)从下层第二键和口(342)引至第二焊盘(334)。 8. The micro NMR probe according to claim 3, wherein: said upper wire (13) and the lower wire (33) to an upper layer by plating plated substrate (11) and the underlying substrate (31), the upper wire (13) lead respectively from both ends of the upper coil (12) adjacent to both sides of an upper substrate (11); a first lower conductor (331), respectively, and causing the lower port of the first key (341) and from both ends of the lower coil (32) a pad (333), a second lower conductor (332) (342) leading from the lower layer to a second port and a second key pad (334).
9.根据权利要求8所述的微型核磁共振探头,其特征在于:所述上层第一键和口( 141)、中层第一键和口(21)、下层第一键和口(341)截面尺寸与第一导体柱(41)相同,上层第二键和口( 142)、中层第二键和口(22)、下层第二键和口(342)截面尺寸与第二导体柱(42)相同。 According to claim micro NMR probe of claim 8, wherein: said first key and the upper port (141), a first key and the middle port (21), a first key and a lower opening (341) cross-section size of the first conductive post (41) the same as the upper opening and the second key (142), the middle and the second key opening (22), (342) a second key-sectional dimension of the lower conductor post and the second port (42) the same.
10.根据权利要求9所述的微型核磁共振探头,其特征在于:所述检测腔(53)为圆柱状,直径为上层基底柱(111)直径的0.5-0.8倍。 10. The micro NMR probe according to claim 9, wherein: said detection chamber (53) is cylindrical column having a diameter of the upper substrate (111) is 0.5-0.8 times the diameter.
Beschreibung  übersetzt aus folgender Sprache: Chinesisch
一种微型核磁共振探头 A miniature nuclear magnetic resonance probe

技术领域 FIELD

[0001]本发明涉及一种核磁共振探头,特别涉及一种利用一次性芯片的微型核磁共振探头。 [0001] The present invention relates to a nuclear magnetic resonance probe, and more particularly to micro NMR probe utilizing disposable chip.

背景技术 Background technique

[0002]核磁共振成像法与核磁共振图谱法作为两种最为通用且信号丰富的检测手段,被广泛的应用在化学、药学、生物与医学领域,由于这一技术的非侵害性,使它尤其适用于生物样品的检测。 [0002] NMR nuclear magnetic resonance imaging methods and atlas method as the two most common and abundant signal detection means, are widely used in the chemical, pharmaceutical, and bio-medical field, since this non-invasive technique, in particular it suitable for detecting a biological sample. 根据经典的信噪比公式(Hoult DI, Richards R E.The signal-to-noise rat1 of the nuclear magnetic resonance experiment.1976.[J].Journalof Magnetic Resonance, 2011, 213(2):329-43.)可知,当被检测样品体积极小时,核磁共振检测的灵敏度可极大提升。 The classic SNR equation (Hoult DI, Richards R E.The signal-to-noise rat1 of the nuclear magnetic resonance experiment.1976 [J] .Journalof Magnetic Resonance, 2011, 213 (2):. 329-43. ) shows that, when the positive test sample body hours, NMR detection sensitivity can be greatly improved. 为此,学者们进行了诸多的尝试,如Roger等(Usingmicrocontact printing to fabricate microcoils on capillaries for highresolut1n proton nuclear magnetic resonance on nanoliter volumes[J].AppliedPhysics Letters, 1997,70( 18):2464-2466.)在毛细管上利用微接触打印与湿法化学腐蚀完成螺线管线圈的绕制,Trumbull等(Integrating microfabricated fluidic systemsand NMR spectroscopy.[J].1EEE transact1ns on b1-medical engineering, 2000,47(1):3-7.)实现了电泳芯片与核磁共振平面螺旋线圈的结合,吴卫平等(The design andfabricat1n of a low-field NMR probe based on a multilayer planar microcoil[J].Microsystem Technologies, 2013,20(3):419-425.)在玻璃衬底上利用MEMS 技术制作集成微流通道的平面线圈,中国专利CN201310659209.4(基于印刷电路板螺线管线圈的低场核磁共振探头)将检测腔集成到印刷电路螺线管线圈内部。 To this end, scholars have made a number of attempts, such as Roger et (Usingmicrocontact printing to fabricate microcoils on capillaries for highresolut1n proton nuclear magnetic resonance on nanoliter volumes [J] .AppliedPhysics Letters, 1997,70 (18):. 2464-2466) in microcontact printing using a capillary with a wet chemical etch to complete wound solenoid coil, Trumbull, etc. (Integrating microfabricated fluidic systemsand NMR spectroscopy [J] .1EEE transact1ns on b1-medical engineering, 2000,47 (1).: 3-7.) to achieve the electrophoresis chip combined with the planar spiral coil of the magnetic resonance, and the like WU Weiping (the design andfabricat1n of a low-field NMR probe based on a multilayer planar microcoil [J] .Microsystem Technologies, 2013,20 (3 ): 419-425) using a planar coil MEMS technology integrated microfluidic channel on a glass substrate, Chinese Patent CN201310659209.4 (low-field NMR probe on the printed circuit board solenoid coil) is integrated into the detection chamber internal solenoid coil printed circuit.

[0003]上述公知实例详细叙述了微型核磁共振探头的工作机理与应用前景,但由于结构限制,样品处理被忽视,探头线圈均与检测腔集成一体。 [0003] The well-known examples illustrate the working mechanism and application prospect micro NMR probe, but because of structural limitations, sample handling is ignored, the probe coils are integrated with the detection chamber. 因此,难以解决探头检测腔不可更换带来的检测腔清洗耗时、样品交叉污染的问题,这一问题在检测超低浓度样品时尤其突出。 Thus, it is difficult to solve the non-replaceable probe detection chamber cleaning time consuming to bring the detection chamber, the sample cross-contamination problems, this is particularly a problem in the detection of ultra-low concentration of the sample.

发明内容 SUMMARY

[0004]( — )要解决的技术问题 [0004] (-) technical problem to be solved

本发明所要解决的技术问题是针对上述已有技术的不足而提供的一种利用一次性芯片的微型核磁共振探头。 The present invention solves the technical problem for less than the above prior art to provide a disposable chip using micro NMR probe.

[0005] (二)技术方案 [0005] (ii) Technical Solution

为解决上述技术问题,本发明的技术方案如下: To solve the above technical problem, the technical solution of the present invention is as follows:

一种微型核磁共振探头,包括探头本体以及置于探头本体内的检测通道,所述检测通道包括样品入口、样品出口和连接在样品入口与样品出口之间的检测腔,其特征在于:所述探头本体包括探头上层、中层垫片、以及探头下层,在所述中层垫片上设置有一可与探头本体外部连通的芯片槽,在该芯片槽内设置有一载样芯片,所述检测通道设置在所述载样芯片上,在所述探头上层设置有上层线圈,在所述探头下层设置有下层线圈,所述上层线圈与下层线圈电连接,所述检测腔位于所述上层线圈与下层线圈之间且三者同心。 A miniature nuclear magnetic resonance probe comprising a probe body and a probe disposed within the body of the detection channel, the detection channel comprises a sample inlet, a sample outlet connected to the detection chamber, and between the sample inlet and the sample outlet, characterized in that: said the probe includes a probe body upper, middle pad, and lower the probe, the chip is provided with a groove communicating with the outside of the probe body may be in the middle of the spacer, in which the chip carrier is provided with a groove-like chip, a detection channel provided loading the chip carrier, the probe is provided with an upper layer of the upper coil, the lower coil is provided with a probe in the lower layer, the upper coil and the lower coil are electrically connected to said detection cavity is in the upper and lower coil of the coil and between the three concentric.

[0006]所述探头上层还包含上层基底、上层导线以及上封装层,所述上层线圈绕在位于上层基底中部的上层基底柱上,上封装层相邻两边切出上层第一键和口和上层第二键和口,所述上层导线设置在第一键和口和上层第二键和口与上层线圈之间;所述中层垫片相邻两边对应上封装层相同位置切出中层第一键和口和中层第二键和口,中层垫片中部设置所述芯片槽;所述探头下层包含下层基底、下层导线以及下封装层,所述下层线圈绕在位于下层基底中部的下层基底柱上,下封装层相相邻两边对应上封装层相同位置切出下层第一键和口和下层第二键和口,所述上层导线与所述下层导线由第一导体柱和第二导体柱进行电导通,整体构成亥姆霍兹线圈。 [0006] The probe further comprises an upper substrate upper layer, the upper layer and the wire layer on the package, the upper coil is wound around the upper substrate located in the upper middle of the column substrate, the encapsulation layer adjacent sides and cut the upper layer of the first key and port the second key and the upper opening, the upper wire disposed between the first key and the second key opening and the upper opening and the upper coil; spacer adjacent sides corresponding to the middle position in the same package a first middle layer is cut key and a second key opening and the middle port and middle gasket groove arranged in the middle of the chip; a substrate underlying said probe comprises a lower layer, the lower layer and a lower conductor layer package, the lower coil is wound around the underlying substrate in the middle column of the underlying substrate the upper layer wires and the column wires of the first lower conductor and the second conductor post on either side of the encapsulating layer corresponding to the same position relative to the adjacent layer of the package was cut out and the lower port of the first key and the second key and the lower opening, electrical conduction, the overall configuration of a Helmholtz coil. 本发明第一导体柱和第二导体柱位于探头本体的相邻两侦U,另外两个相邻的边分别用作了芯片槽和焊盘,这样结构比较紧凑,也利于导线的优化布置,促进检测探头的微型化和提高导线对检测的精度的影响。 The first conductor post and the second conductor post of the present invention is positioned adjacent the probe body investigation two U, the other two adjacent sides are used as the chip groove and the pad, so that relatively compact structure, but also help to optimize the arrangement of the wires, influence on the accuracy of the detection probes to facilitate detection of miniaturization and improved wire.

[0007]所述下层导线包括下层第一导线和下层第二导线,所述下层第一导线连接在第一焊盘与下层第一键和口之间并与所述下层线圈连接,所述下层第二导线连接在第二焊盘与下层第二键和口之间。 [0007] The lower layer of the first lead wire and a lower layer comprising a second conductor, the first conductor connected between the lower pad and the first key and the first port and the lower coil connected to the lower layer, the lower layer a second wire connected between the second pad and the second key and the lower port.

[0008]下封装层覆盖区域不包含第一焊盘与第二焊盘。 [0008] The coverage area of the encapsulation layer does not comprise a first pad and the second pad.

[0009]所述上层基底和下层基底材料为硬质非金属材料;所述上封装层与下封装层材料为光固化材料;所述上层线圈、上层导线、下层线圈和下层导线材料为高导电材料。 [0009] The upper substrate and the lower substrate material is a hard non-metallic material; encapsulating the upper layer and the lower layer of encapsulating material is a photocurable material; the upper coil, the upper wire and the lower wire lower coil highly conductive material material.

[0010]所述硬质非金属材料为玻璃或聚甲基丙烯酸甲酯;所述光固化材料为UV树脂或SU-8;所述高导电材料为铜、银或金。 The [0010] the non-metallic material is glass or polymethyl methacrylate; the photocurable resin material is a UV or SU-8; the highly conductive material is copper, silver or gold.

[0011]所述探头上层、中层垫片、探头下层相邻两层间结合方式为不可逆的等离子体氧化粘结或高分子材料粘结。 The [0011] probe upper, middle pad, probe binding manner between the two layers adjacent to the lower irreversible plasma oxidation or polymer material bonded adhesive.

[0012]所述上层导线与下层导线通过电镀铺到上层基底与下层基底上,上层导线分别从上层线圈两端引致相邻上层基底两边;下层第一导线分别从下层线圈两端引致下层第一键和口和第一焊盘,下层第二导线从下层第二键和口引至第二焊盘。 [0012] The upper and lower wire laying wire by plating on the upper substrate and the lower substrate, an upper layer lead, respectively, on both sides of the substrate adjacent to the upper ends of the coil lead; a first lower layer of the first lead wires respectively from the lower ends of the coil and the first pad and the key port, a second lower wire lead from the lower layer to a second port and a second key pad.

[0013]所述上层第一键和口、中层第一键和口、下层第一键和口截面尺寸与第一导体柱相同,上层第二键和口、中层第二键和口、下层第二键和口截面尺寸与第二导体柱相同。 [0013] The upper port and a first key, middle key and a first port, a first key and the lower opening cross-sectional dimension identical to the first conductor post, and the upper opening of the second key, middle key and a second port, the first lower two keys and the second port cross-sectional dimensions of the same conductor post.

[0014] 所述检测腔为圆柱状,直径为上层基底柱直径的0.5-0.8倍。 [0014] The detection chamber is cylindrical, having a diameter of 0.5-0.8 times the column diameter of the upper substrate. 该区域内亥姆霍兹线圈的射频场均匀度最佳。 Helmholtz coil radio frequency field uniformity within the region best.

[0015]所述载样芯片工作时完全插入芯片槽,在完成单样品检测后即抛弃,不再重复利用。 [0015] When fully inserted into the loaded working silicon chip groove, discarded after the completion of a single sample testing, not repeated use. 本发明微型核磁共振探头,在实现微量样品检测的同时,保证样品无残留、探头不被污染,能获取更精确可靠的检测信号。 Micro NMR probe of the present invention, while achieving the micro sample to be tested to ensure no residue sample, the probe will not be contaminated and can obtain more accurate and reliable signal detection.

[0016](三)有益效果本发明一种利用一次性芯片的核磁共振探头,在满足微型核磁共振探头高信噪比的同时,给出了探头线圈与检测腔隔离结构的设计方案,载样芯片在每次样品检测后抛弃,从根源上避免了样品残留在探头中带来的交叉污染,使得探头主体部分可长期重复利用,兼具高灵敏度与经济性。 [0016] (iii) the beneficial effects of the present invention utilizing a disposable chip NMR probe, while satisfying high SNR micro NMR probe, the probe coil design scheme of the test chamber and the isolation structure, like the carrier after each chip sample detection discarded, to avoid cross-contamination caused by residual samples from the source in the probe, the probe body portion such that long-term repeated use, both high sensitivity and economy. 同时,本发明磁共振探头,采用多层分立式设计,解决了常规一体式探头制造难度大的问题,在实现“可替换”的前提下,制造流程简易可靠,大大降低了制造成本,提高了生产效率。 Meanwhile, the magnetic resonance probe of the present invention, a discrete multi-layer designed to solve the problems of the conventional one great difficulty producing probe, under the precondition "alternative", the manufacturing process simple and reliable, greatly reducing manufacturing costs and improving production efficiency.

附图说明 BRIEF DESCRIPTION

[0017]图1为本发明的整体结构爆炸示意图; [0017] FIG. 1 is an overall configuration of an exploded diagram of the present invention;

图2为本发明的轴侧示意图; Axis side in FIG. 2 a schematic view of the present invention;

图3为本发明中探头上层的结构示意图; 3 is a schematic view of the structure of the upper layer of the invention, FIG probe;

图4为本发明中中层垫片的结构示意图; 4 a schematic view of the middle structure of the spacer in the present invention, FIG;

图5为本发明中探头下层的结构示意图; 5 is a schematic structural diagram of a probe lower invention;

图6为本发明实施例检测状态的轴侧示意图; Example 6 is a schematic side axis detection state embodiment of the invention;

图中:I探头上层、11上层基底、111上层基底柱、12上层线圈、13上层导线、14上封装层、141上层第一键和口、142上层第二键和口、2中层垫片、21中层第一键和口、22中层第二键和口、23芯片槽、3探头下层、31下层基底、311下层基底柱、32下层线圈、33下层导线、331下层第一导线、332下层第二导线、333第一焊盘、334第二焊盘、34下封装层、341下层第一键和口、342下层第二键和口、41第一导体柱、42第二导体柱、5载样芯片、51样品入口、52样品出口、53检测腔。 FIG Where: I probe upper, upper substrate 11, the upper base column 111, the upper coil 12, the upper wire 13, 14 on the encapsulation layer 141 and the upper port of the first key, second key and the upper port 142, the middle shim 2, middle port 21 and the first key, second key and the middle port 22, the chip groove 23, the lower the probe 3, 31 underlying substrate, the underlying substrate 311 column lower coil 32, the lower wire 33, a first lower conductor 331, 332 of the lower two wires, a first pad 333, a second pad 334, the encapsulation layer 34, a first key 341 and the lower port 342 and the lower port of the second key, the first conductor post 41, a second conductive post 42, the carrier 5 sample chip, the sample inlet 51, sample outlet 52, the detection chamber 53.

具体实施方式 detailed description

[0018]为使本发明的上述目的、特征和优点能够更加清晰易懂,下面结合附图对本发明的具体实施方式做详细的说明。 [0018] For the above-described objects, features and advantages of the present invention can be more clearly understood, the accompanying drawings in conjunction with the following detailed description of specific embodiments of the present invention.

[0019]请参阅图1-5,本发明微型核磁共振探头,其具有:探头上层1、中层垫片2、探头下层3和载样芯片5。 [0019] Referring to FIG 1-5, the present invention is micro NMR probe comprising: a probe the upper, middle pad 2, the probe 3 and the lower chip carrier 5 comp. 所述探头上层I包含上层基底11、上层线圈12、上层导线13和上封装层14,上层线圈12绕在位于上层基底11中部的上层基底柱111上,上封装层14相邻两边切出上层第一键和口141和上层第二键和口142,露出部分上层导线13;所述中层垫片2相邻两边对应上封装层14相同位置切出中层第一键和口21和中层第二键和口22,中层垫片2中部切出芯片槽23;所述探头下层3包含下层基底31、下层线圈32、下层导线33以及下封装层34,下层线圈绕32在位于下层基底31中部的下层基底柱上311,下封装层34覆盖区域不包含第一焊盘333与第二焊盘334,并且下封装层34相相邻两边对应上封装层14相同位置切出下层第一键和口341和下层第二键和口342,露出部分下层第一导线331和下层第二导线332;上层导线13与下层导线33由第一导体柱41和第二导体柱42进行电导通,整体构成亥姆霍兹线圈;载样芯片5至 The upper layer I comprises upper probe substrate 11, the upper coil 12, the upper conductor layer 13 and the package 14, the upper coil 12 wound on the upper substrate 11 located in the middle column of the upper substrate 111, the encapsulation layer 14 adjacent to the sides of the upper cut-out the first key and the second key and the upper port 141 and port 142, the exposed portion of the upper wire 13; the middle pad 2 corresponds to the same position on both sides adjacent encapsulation layer 14 is cut out port 21 and the middle of the first key and a second middle layer key and opening 22, the middle die pad 2 central groove 23 cut; said probe comprises a lower layer 3 underlying substrate 31, the lower coil 32, the lower conductor 33 and a lower encapsulation layer 34 around the lower coil 32 is located in the middle of the lower base 31 column 311 underlying substrate, the encapsulation layer 34 covers the region does not include a first pad 333 and second pad 334, and the lower the encapsulation layer 34 adjacent to the corresponding sides of the position of the encapsulation layer 14 on the same layer of the first key cut and port and the lower keys 341 and a second port 342, the lower exposed portion of the first conductor 331 and second conductor 332 lower; upper wire 13 and lower wire 33 by the first electrically conductive conductor post 41 and the second conductor post 42, the overall configuration Hai Helmholtz coils; chip 5 to the loaded 少包含样品入口51、样品出口52和检测腔53。 Containing at least the sample inlet 51, outlet 52 and the sample detection chamber 53.

[0020] 实施例: [0020] Example:

请参见图2与图6,本案提供的一种利用一次性芯片的核磁共振探头,使用过程如下:1、采用普遍使用的去嵌入法,利用第一焊盘333与第二焊盘334接入阻抗分析仪,测试本发明中上层线圈12、下层线圈32、上层导线13、下层导线33、第一导电柱41和第二导电柱42组成的亥姆霍兹线圈在拉莫频率下的阻抗值;2、接入实际电路,利用L性振荡电路匹配至50欧姆;3、将调谐过的探头放置到主磁场中,主要为永磁体提供的静磁场;4、将液体样品通过样品入口51通入未使用载样芯片检测腔53,持续稳定通至样品出口52有微量样品溢出,稳定3-5秒钟;5、将该载样芯片5插入芯片槽23直到遇到阻力,保证检测腔53位于亥姆霍兹线圈中心位置;6、将探头部分与核磁共振控制模块连接,在上位机上操作控制软件,获取样品核磁共振信号;7、通过对这些信号的处理与分析得到样品分子 See Figure 2 and 6, the case using a disposable chip to provide nuclear magnetic resonance probe, using the following procedure: 1 using de-embedding method commonly used by the first pad 333 and second pad 334 access impedance analyzer test of the present invention, the upper coil 12, lower coil 32, the upper conductor 13, lower conductor 33, the first conductive pillar 41 and the second conductive pillar 42 consisting of a Helmholtz coil impedance value at a Larmor frequency ; 2, the actual access circuit, using L of oscillation circuit matched to 50 ohms; 3, the probe is placed into the tuned main magnetic field, static magnetic field of the permanent magnet is mainly provided; 4, the liquid sample through the sample inlet 51 through the loaded chip into the detection chamber 53 is not used, a steady outlet 52 to the sample through the sample trace overflow stable 3-5 seconds; 5, the chip carrier 5 is inserted into the groove-like chip 23 until it meets resistance, to ensure that the test chamber 53 Helmholtz coil was located; 6, nuclear magnetic resonance and the probe portion control module, control software operating on a host computer, the sample acquiring nuclear magnetic resonance signals; 7, by processing and analysis of the signals obtained sample molecules 构层面的性质;8、完成单样品检测后,抛弃已使用载样芯片5。 Level properties configuration; 8, after the completion of a single sample detection, discard the loaded chip 5 has been used.

[0021]以上所述仅是本发明的优选实施方案,应当指出:对于本技术领域的普通技术人员来说,在不脱离本发明的前提下,还可做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。 [0021] The above are only preferred embodiments of the present invention, it should be noted: to those of ordinary skill in the art who, without departing from the present invention may make various improvements and modifications, such modifications and modifications should be the scope of the present invention.

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Klassifizierungen
Internationale KlassifikationG01N24/08
Juristische Ereignisse
DatumCodeEreignisBeschreibung
7. Dez. 2016C06Publication
4. Jan. 2017C10Entry into substantive examination