CN106198598A - A kind of Micro Core magnetic resonance probe - Google Patents
A kind of Micro Core magnetic resonance probe Download PDFInfo
- Publication number
- CN106198598A CN106198598A CN201610536344.3A CN201610536344A CN106198598A CN 106198598 A CN106198598 A CN 106198598A CN 201610536344 A CN201610536344 A CN 201610536344A CN 106198598 A CN106198598 A CN 106198598A
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- probe
- lower floor
- mouth
- key
- upper strata
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N24/00—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects
- G01N24/08—Investigating or analyzing materials by the use of nuclear magnetic resonance, electron paramagnetic resonance or other spin effects by using nuclear magnetic resonance
Abstract
The invention discloses a kind of Micro Core magnetic resonance probe, including probe body and the sense channel that is placed in probe body, described sense channel includes sample inlet, sample export and the test chamber being connected between sample inlet and sample export, it is characterized in that: described probe body includes upper strata of popping one's head in, middle level pad, and probe lower floor, being provided with one on the pad of described middle level can be with the chip slot of probe body ft connection, a load sample chip it is provided with in this chip slot, described sense channel is arranged on described load sample chip, described probe upper strata is provided with upper coil, described probe lower floor is provided with inner coil, described upper coil electrically connects with inner coil, described test chamber is between described upper coil and inner coil and three is concentric.Micro Core magnetic resonance probe of the present invention, while realizing micro-example detection, it is ensured that sample noresidue, probe are not contaminated, and can obtain and more accurately detect signal reliably.
Description
Technical field
The present invention relates to a kind of nmr probe, visit particularly to a kind of Micro Core magnetic resonance utilizing disposable chip
Head.
Background technology
NMR imaging method and the detection means that nuclear magnetic resonance map method is the most general as two kinds and signal is abundant, quilt
It is widely applied to chemistry, pharmacy, biology and medical domain, non-invasive due to this technology, make it be particularly suited for raw
The detection of thing sample.According to classical signal to noise ratio formula (Hoult D I, Richards R E. The signal-to-
noise ratio of the nuclear magnetic resonance experiment. 1976.[J]. Journal
Of Magnetic Resonance, 2011,213 (2): 329-43.) understand, when detected sample volume is minimum, nuclear-magnetism
The sensitivity of resonance detection can significant increase.To this end, scholars have carried out many trials, such as (Using such as Roger
microcontact printing to fabricate microcoils on capillaries for high
resolution proton nuclear magnetic resonance on nanoliter volumes[J]. Applied
Physics Letters, 1997,70 (18): 2464-2466.) utilize micro-contact to print and wet chemistry corruption on the capillary
Lose the coiling of solenoid coil, (the Integrating microfabricated fluidic systems such as Trumbull
and NMR spectroscopy.[J]. IEEE transactions on bio-medical engineering, 2000,
47 (1): 3-7.) combination of electrophoresis chip and nuclear magnetic resonance, NMR planar spiral winding, Wu Wei equality (The design and are achieved
fabrication of a low-field NMR probe based on a multilayer planar microcoil
[J]. Microsystem Technologies, 2013,20 (3): 419-425.) utilize MEMS technology on a glass substrate
Making the planar coil of integrated microchannel, Chinese patent CN201310659209.4(is based on printed circuit board (PCB) solenoid coil
Low-field nuclear magnetic resonance probe) test chamber is integrated into inside printed circuit solenoid coil.
Above-mentioned well known examples describes working mechanism and the application prospect of Micro Core magnetic resonance probe in detail, but due to structure
Limiting, sample treatment is out in the cold, and probe coil is all integrated with test chamber.Can not be more accordingly, it is difficult to solve probe test chamber
The test chamber that tape swapping comes cleans time-consuming, the problem of sample cross contamination, and this problem is the most prominent when detecting super low concentration sample
Go out.
Summary of the invention
(1) to solve the technical problem that
The technical problem to be solved is the deficiency for above-mentioned prior art and the one that provides utilizes disposable core
The Micro Core magnetic resonance probe of sheet.
(2) technical scheme
For solving above-mentioned technical problem, technical scheme is as follows:
A kind of Micro Core magnetic resonance probe, including probe body and the sense channel that is placed in probe body, described detection is led to
Road includes sample inlet, sample export and the test chamber being connected between sample inlet and sample export, it is characterised in that: described
Probe body includes pop one's head in upper strata, middle level pad and probe lower floor, and being provided with one on the pad of described middle level can with probe originally
The chip slot of external body connection, is provided with a load sample chip in this chip slot, and described sense channel is arranged on described load sample core
On sheet, described probe upper strata is provided with upper coil, described probe lower floor is provided with inner coil, described upper coil with
Inner coil electrically connects, and described test chamber is between described upper coil and inner coil and three is concentric.
Described probe upper strata also comprises upper strata substrate, topping wire and upper encapsulated layer, and described upper coil is wound on and is positioned at
On upper strata substrate post in the middle part of the substrate of upper strata, the adjacent both sides of upper encapsulated layer cut out upper strata the first key and mouth and upper strata the second key and
Mouthful, described topping wire is arranged between the first key and mouth and upper strata the second key and mouth and upper coil；Described middle level pad phase
In the correspondence of adjacent both sides, encapsulated layer same position cuts out middle level the first key and mouth and middle level the second key and mouth, arranges in the middle part of the pad of middle level
Described chip slot；Described probe lower floor comprises lower substrate, lower layer conductor and lower encapsulated layer, and described inner coil is wound on and is positioned at
On lower substrate post in the middle part of lower substrate, in the correspondence of lower encapsulated layer mutually adjacent both sides, encapsulated layer same position cuts out lower floor first
Key and mouth and lower floor's the second key and mouth, described topping wire is carried out by the first conductor pin and the second conductor pin with described lower layer conductor
Conduct, be monolithically fabricated Helmholtz coil.The present invention the first conductor pin and the second conductor pin are positioned at adjacent the two of probe body
Side, two other adjacent limit has been used separately as chip slot and pad, and such structure comparison is compact, the most beneficially the optimization cloth of wire
Put, promote the miniaturization of detection probe and improve the wire impact on the precision of detection.
Described lower layer conductor includes that lower floor's the first wire and lower floor's the second wire, described lower floor the first wire are connected to first
Be connected between pad with lower floor's the first key and mouth and with described inner coil, described lower floor the second wire be connected to the second pad with
Between lower floor's the second key and mouth.
Lower encapsulated layer overlay area does not comprise the first pad and the second pad.
The substrate of described upper strata and lower substrate material are hard non-metallic material；Described upper encapsulated layer and lower encapsulated layer material
For photo-curing material；Described upper coil, topping wire, inner coil and lower floor's conductor material are high conductive material.
Described hard non-metallic material is glass or polymethyl methacrylate；Described photo-curing material be UV resin or
SU-8；Described high conductive material is copper, silver-colored or golden.
Between described probe upper strata, middle level pad, probe lower floor adjacent two layers, combination is irreversible plasma oxygen
Change bonding or macromolecular material bonding.
Described topping wire and lower layer conductor are taped against in upper strata substrate and lower substrate by plating, topping wire respectively from
Upper coil two ends cause adjacent upper substrate both sides；Lower floor's the first wire causes lower floor's the first key respectively from inner coil two ends
With mouth and the first pad, lower floor's the second wire causes the second pad from lower floor's the second key and mouth.
Described upper strata the first key and mouth, middle level the first key and mouth, lower floor's the first key and mouth sectional dimension and the first conductor pin
Identical, upper strata the second key and mouth, middle level the second key and mouth, lower floor's the second key are identical with the second conductor pin with mouth sectional dimension.
Described test chamber is cylindric, 0.5-0.8 times of a diameter of upper strata substrate column diameter.Helmholtz's line in this region
The radio-frequency field uniformity of circle is optimal.
Fully-inserted chip slot during described load sample chip operation, i.e. abandons after completing monocyte sample detection, profit is not repeated
With.Micro Core magnetic resonance probe of the present invention, realize micro-example detection while, it is ensured that sample noresidue, probe the most dirty
Dye, can obtain and more accurately detect signal reliably.
(3) beneficial effect
A kind of nmr probe utilizing disposable chip of the present invention, is meeting the same of Micro Core magnetic resonance probe high s/n ratio
Time, giving the design of probe coil and test chamber isolation structure, load sample chip is abandoned after each sample detection, from root
The cross-contamination that sample carryover brings in probe is avoided so that probe body part can reuse for a long time, has concurrently on source
High sensitivity and economy.Meanwhile, magnetic resonance probe of the present invention, use the design of multilamellar discrete, solve conventional integral and visit
The problem that head manufacture difficulty is big, on the premise of realizing " replaceable ", manufacturing process is simply reliable, greatly reduces and is manufactured into
This, improve production efficiency.
Accompanying drawing explanation
Fig. 1 is the overall structure exploded perspective view of the present invention；
Fig. 2 is the axle side schematic diagram of the present invention；
Fig. 3 is the structural representation on upper strata of popping one's head in the present invention；
Fig. 4 is the structural representation of middle level pad in the present invention；
Fig. 5 is the structural representation of lower floor of popping one's head in the present invention；
Fig. 6 is the axle side schematic diagram of embodiment of the present invention detection state；
In figure: 1 probe upper strata, 11 upper strata substrates, 111 upper strata substrate posts, 12 upper coils, 13 topping wire, encapsulated layer on 14,
141 upper strata the first keys and mouth, 142 upper strata the second keys and mouth, 2 middle level pads, 21 middle level the first keys and mouth, 22 middle level the second keys and
Mouth, 23 chip slot, 3 probe lower floor, 31 lower substrate, 311 lower substrate posts, 32 inner coils, 33 times layer conductors, 331 lower floors
First wire, 332 lower floor's the second wires, 333 first pads, 334 second pads, 34 times encapsulated layers, 341 lower floor's the first keys and
Mouth, 342 lower floor's the second keys and mouth, 41 first conductor pins, 42 second conductor pins, 5 load sample chips, 51 sample inlets, 52 samples go out
Mouth, 53 test chamber.
Detailed description of the invention
Understandable, below in conjunction with the accompanying drawings to the present invention for enabling the above-mentioned purpose of the present invention, feature and advantage to become apparent from
Detailed description of the invention be described in detail.
Referring to Fig. 1-5, Micro Core magnetic resonance probe of the present invention, it has: under probe upper strata 1, middle level pad 2, probe
Layer 3 and load sample chip 5.Described probe upper strata 1 comprises upper strata substrate 11, upper coil 12, topping wire 13 and upper encapsulated layer 14,
Upper coil 12 is wound on the upper strata substrate post 111 being positioned in the middle part of upper strata substrate 11, and the upper adjacent both sides of encapsulated layer 14 cut out upper strata
First key and mouth 141 and upper strata the second key and mouth 142, exposed portion topping wire 13；The adjacent both sides of described middle level pad 2 are corresponding
Upper encapsulated layer 14 same position cuts out middle level the first key and mouth 21 and middle level the second key and mouth 22, cuts out core in the middle part of middle level pad 2
Film trap 23；Described probe lower floor 3 comprises lower substrate 31, inner coil 32, lower layer conductor 33 and lower encapsulated layer 34, lower layer line
Enclosing around 32 on the lower substrate post being positioned in the middle part of lower substrate 31 311, lower encapsulated layer 34 overlay area does not comprise the first pad
333 and second pad 334, and in the correspondence of lower encapsulated layer 34 mutually adjacent both sides, encapsulated layer 14 same position cuts out lower floor's the first key
With mouth 341 and lower floor's the second key and mouth 342, exposed portion lower floor the first wire 331 and lower floor's the second wire 332；Topping wire
13 are conducted by the first conductor pin 41 and the second conductor pin 42 with lower layer conductor 33, are monolithically fabricated Helmholtz coil；Carry
Sample chip 5 is including at least sample inlet 51, sample export 52 and test chamber 53.
Embodiment:
Refer to Fig. 2 and Fig. 6, a kind of nmr probe utilizing disposable chip that this case provides, use process as follows: 1,
Use the De-embedding method commonly used, utilize the first pad 333 and the second pad 334 termination analyser, test the present invention
Coil 12, inner coil 32, topping wire 13, lower layer conductor the 33, first conductive pole 41 and the second conductive pole 42 form at the middle and upper levels
Helmholtz coil resistance value under Rameau frequency；2, access side circuit, utilize L oscillating circuit to be matched to 50 Europe
Nurse；3, by tuned probe placement to main field, the magnetostatic field that predominantly permanent magnet provides；4, fluid sample is passed through sample
Product entrance 51 is passed through and does not uses load sample chip detection chamber 53, and passing to sample export 52 continually and steadily has micro-example to overflow, stable
3-5 second；5, this load sample chip 5 is inserted chip slot 23 until meeting obstructions, it is ensured that test chamber 53 is positioned at Helmholtz coil
Center；6, probe segment is connected with nuclear magnetic resonance, NMR control module, operation control software on host computer, obtains sample core
Magnetic resonance signal；7, by the process of these signals and analysis being obtained the character of sample molecule structural level；8, single sample is completed
After product examine is surveyed, abandon and use load sample chip 5.
The above is only the preferred embodiments of the invention, it should be pointed out that: for the ordinary skill people of the art
For Yuan, under the premise of not departing from the present invention, it may also be made that some improvements and modifications, these improvements and modifications also should be regarded as this
The protection domain of invention.
Claims (10)
1. a Micro Core magnetic resonance probe, including probe body and the sense channel that is placed in probe body, described detection
Passage includes sample inlet (51), sample export (52) and the test chamber (53) being connected between sample inlet and sample export,
It is characterized in that: described probe body includes pop one's head in upper strata (1), middle level pad (2) and probe lower floor (3), in described middle level
It is provided with one on pad and can be provided with a load sample chip (5), institute in this chip slot with the chip slot of probe body ft connection
State sense channel to be arranged on described load sample chip, described probe upper strata is provided with upper coil, set in described probe lower floor
Being equipped with inner coil, described upper coil electrically connects with inner coil, and described test chamber is positioned at described upper coil and lower layer line
Between circle and three is concentric.
Micro Core magnetic resonance probe the most according to claim 1, it is characterised in that: described probe upper strata (1) also comprises
Layer substrate (11), topping wire (13) and upper encapsulated layer (14), described upper coil (12) is wound on and is positioned at upper strata substrate (11)
In upper strata substrate post (111) at middle part, upper encapsulated layer (14) adjacent both sides cut out upper strata the first key and mouth (141) and upper strata second
Key and mouth (142), described topping wire (13) is arranged on the first key and mouth (141) and upper strata the second key and mouth (142) and upper strata
Between coil (12)；In the correspondence of pad (2) adjacent both sides, described middle level encapsulated layer (14) same position cut out middle level the first key and
Mouthful (21) and middle level the second key and mouth (22), arrange described chip slot (23) in the middle part of middle level pad (2)；Described probe lower floor (3)
Comprising lower substrate (31), lower layer conductor (33) and lower encapsulated layer (34), described inner coil (32) is wound on and is positioned at lower floor's base
On the lower substrate post at middle part, the end (31) (311), encapsulated layer (14) same position in the correspondence of lower encapsulated layer (34) mutually adjacent both sides
Cut out lower floor's the first key and mouth (341) and lower floor's the second key and mouth (342), described topping wire (13) and described lower layer conductor
(33) conducted by the first conductor pin (41) and the second conductor pin (42), be monolithically fabricated Helmholtz coil.
Micro Core magnetic resonance probe the most according to claim 2, it is characterised in that: described lower layer conductor includes lower floor first
Wire (331) and lower floor's the second wire (332), described lower floor the first wire (331) is connected to the first pad (333) and lower floor the
Being connected between one key and mouth (341) and with described inner coil (32), described lower floor the second wire (332) is connected to the second pad
(334) and between lower floor's the second key and mouth (342).
Micro Core magnetic resonance probe the most according to claim 2, it is characterised in that: lower encapsulated layer (34) overlay area is not wrapped
Containing the first pad (333) and the second pad (334).
5. according to the arbitrary described Micro Core magnetic resonance probe of claim 1-4, it is characterised in that: described upper strata substrate (11) and
Lower substrate (31) material is hard non-metallic material；Described upper encapsulated layer (14) and lower encapsulated layer (34) material are photocuring material
Material；Described upper coil (12), topping wire (13), inner coil (32) and lower layer conductor (33) material are high conductive material.
Micro Core magnetic resonance probe the most according to claim 5, it is characterised in that: described hard non-metallic material is glass
Or polymethyl methacrylate；Described photo-curing material is UV resin or SU-8；Described high conductive material is copper, silver-colored or golden.
7. according to the arbitrary described Micro Core magnetic resonance probe of claim 1-4, it is characterised in that: described probe upper strata (1), in
Between layer pad (2), probe lower floor (3) adjacent two layers, combination is irreversible plasma oxidation bonding or macromolecular material
Bonding.
Micro Core magnetic resonance probe the most according to claim 3, it is characterised in that: described topping wire (13) is led with lower floor
Line (33) is taped against in upper strata substrate (11) and lower substrate (31) by plating, and topping wire (13) is respectively from upper coil (12)
Two ends cause adjacent upper substrate (11) both sides；Lower floor's the first wire (331) causes lower floor from inner coil (32) two ends respectively
First key and mouth (341) and the first pad (333), lower floor's the second wire (332) causes second from lower floor's the second key and mouth (342)
Pad (334).
Micro Core magnetic resonance probe the most according to claim 8, it is characterised in that: described upper strata the first key and mouth (141),
Middle level the first key is identical with the first conductor pin (41) with mouth (21), lower floor's the first key and mouth (341) sectional dimension, upper strata the second key
Identical with the second conductor pin (42) with mouth (142), middle level the second key and mouth (22), lower floor's the second key and mouth (342) sectional dimension.
Micro Core magnetic resonance probe the most according to claim 9, it is characterised in that: described test chamber (53) is cylindric,
0.5-0.8 times of a diameter of upper strata substrate post (111) diameter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610536344.3A CN106198598B (en) | 2016-07-09 | 2016-07-09 | A kind of miniature nmr probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610536344.3A CN106198598B (en) | 2016-07-09 | 2016-07-09 | A kind of miniature nmr probe |
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| Publication Number | Publication Date |
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| CN106198598A true CN106198598A (en) | 2016-12-07 |
| CN106198598B CN106198598B (en) | 2018-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610536344.3A Active CN106198598B (en) | 2016-07-09 | 2016-07-09 | A kind of miniature nmr probe |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194900B1 (en) * | 1998-06-19 | 2001-02-27 | Agilent Technologies, Inc. | Integrated miniaturized device for processing and NMR detection of liquid phase samples |
| US6326787B1 (en) * | 1999-06-10 | 2001-12-04 | Sandia National Laboratories | NMR of thin layers using a meanderline surface coil |
| CN1942786A (en) * | 2004-04-30 | 2007-04-04 | 波克股份有限公司 | RF probe apparatus for nmr check weighing system |
| WO2009046350A1 (en) * | 2007-10-05 | 2009-04-09 | The Regents Of The University Of California | Detection of magnetic resonance signals using a magnetoresistive sensor |
| CN103923825A (en) * | 2014-04-17 | 2014-07-16 | 东南大学 | Microfluidic chip system integrating cell sorting and detection |
| CN104898078A (en) * | 2015-06-05 | 2015-09-09 | 东南大学 | Miniature solenoid coil probe and manufacturing method thereof |
-
2016
- 2016-07-09 CN CN201610536344.3A patent/CN106198598B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6194900B1 (en) * | 1998-06-19 | 2001-02-27 | Agilent Technologies, Inc. | Integrated miniaturized device for processing and NMR detection of liquid phase samples |
| US6326787B1 (en) * | 1999-06-10 | 2001-12-04 | Sandia National Laboratories | NMR of thin layers using a meanderline surface coil |
| CN1942786A (en) * | 2004-04-30 | 2007-04-04 | 波克股份有限公司 | RF probe apparatus for nmr check weighing system |
| WO2009046350A1 (en) * | 2007-10-05 | 2009-04-09 | The Regents Of The University Of California | Detection of magnetic resonance signals using a magnetoresistive sensor |
| CN103923825A (en) * | 2014-04-17 | 2014-07-16 | 东南大学 | Microfluidic chip system integrating cell sorting and detection |
| CN104898078A (en) * | 2015-06-05 | 2015-09-09 | 东南大学 | Miniature solenoid coil probe and manufacturing method thereof |
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| Publication number | Publication date |
|---|---|
| CN106198598B (en) | 2018-06-29 |
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