WO2009002506A3 - High density molecular alignment of nucleic acid molecules - Google Patents

High density molecular alignment of nucleic acid molecules Download PDF

Info

Publication number
WO2009002506A3
WO2009002506A3 PCT/US2008/007886 US2008007886W WO2009002506A3 WO 2009002506 A3 WO2009002506 A3 WO 2009002506A3 US 2008007886 W US2008007886 W US 2008007886W WO 2009002506 A3 WO2009002506 A3 WO 2009002506A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid molecules
high density
molecular alignment
density molecular
Prior art date
Application number
PCT/US2008/007886
Other languages
English (en)
French (fr)
Other versions
WO2009002506A2 (en
Inventor
William Roy Glover
Original Assignee
Zs Genetics Inc
William Roy Glover
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zs Genetics Inc, William Roy Glover filed Critical Zs Genetics Inc
Publication of WO2009002506A2 publication Critical patent/WO2009002506A2/en
Publication of WO2009002506A3 publication Critical patent/WO2009002506A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/0054—Means for coding or tagging the apparatus or the reagents
    • B01J2219/00572—Chemical means
    • B01J2219/00576—Chemical means fluorophore
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00655—Making arrays on substantially continuous surfaces the compounds being bound to magnets embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
PCT/US2008/007886 2007-06-25 2008-06-25 High density molecular alignment of nucleic acid molecules WO2009002506A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US93706607P 2007-06-25 2007-06-25
US60/937,066 2007-06-25

Publications (2)

Publication Number Publication Date
WO2009002506A2 WO2009002506A2 (en) 2008-12-31
WO2009002506A3 true WO2009002506A3 (en) 2009-07-09

Family

ID=40186218

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/007886 WO2009002506A2 (en) 2007-06-25 2008-06-25 High density molecular alignment of nucleic acid molecules

Country Status (2)

Country Link
TW (1) TW200912307A (de)
WO (1) WO2009002506A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014078652A1 (en) 2012-11-16 2014-05-22 Zs Genetics, Inc. Heavy atom labeled nucleosides, nucleotides, and nucleic acid polymers, and uses thereof
WO2017075179A1 (en) 2015-10-27 2017-05-04 Zs Genetics, Inc. Sequencing by deconvolution
US20220157559A1 (en) * 2019-02-25 2022-05-19 Universiteit Antwerpen Electron microscopy grid

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004027093A1 (en) * 2002-09-19 2004-04-01 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
EP1407816A1 (de) * 2002-09-17 2004-04-14 Humboldt Universität zu Berlin Verfahren zum Anordnen einem Polymer-Molekül
EP1516665A1 (de) * 2003-09-18 2005-03-23 Sony International (Europe) GmbH Verfahren zu der Immobilisierung und dem Spannen einer Nukleinsäure auf ein Substrat
WO2005025737A2 (de) * 2003-09-16 2005-03-24 Upper Austrian Research Gmbh Molekül-arrays und verfahren zu deren herstellung
WO2007120202A2 (en) * 2005-11-09 2007-10-25 Zs Genetics, Inc. Nano-scale ligand arrays on substrates for particle beam instruments and related methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1407816A1 (de) * 2002-09-17 2004-04-14 Humboldt Universität zu Berlin Verfahren zum Anordnen einem Polymer-Molekül
WO2004027093A1 (en) * 2002-09-19 2004-04-01 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
WO2005025737A2 (de) * 2003-09-16 2005-03-24 Upper Austrian Research Gmbh Molekül-arrays und verfahren zu deren herstellung
EP1516665A1 (de) * 2003-09-18 2005-03-23 Sony International (Europe) GmbH Verfahren zu der Immobilisierung und dem Spannen einer Nukleinsäure auf ein Substrat
WO2007120202A2 (en) * 2005-11-09 2007-10-25 Zs Genetics, Inc. Nano-scale ligand arrays on substrates for particle beam instruments and related methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HIDENOBU NAKAO ET AL: "Development of Novel Polymer-coated Substrates for Straightening and Fixing DNA", 1 January 1992, NANO LETTERS, ACS, WASHINGTON, DC, US, PAGE(S) 475 - 479, ISSN: 1530-6984, XP002225163 *
KLEIN D C G ET AL: "Ordered stretching of single molecules of deoxyribose nucleic acid between microfabricated polystyrene lines", APPLIED PHYSICS LETTERS, AIP, AMERICAN INSTITUTE OF PHYSICS, MELVILLE, NY, US, vol. 78, no. 16, 16 April 2001 (2001-04-16), pages 2396 - 2398, XP012027788, ISSN: 0003-6951 *

Also Published As

Publication number Publication date
WO2009002506A2 (en) 2008-12-31
TW200912307A (en) 2009-03-16

Similar Documents

Publication Publication Date Title
HK1257956A1 (zh) 單分子核酸序列分析方法和組合物
EP2294225B8 (de) Verfahren zur direkten amplifikation aus rohen nukeinsäureproben
WO2008069906A3 (en) Digital expression of gene analysis
WO2007146819A3 (en) Methods for identifying and using snp panels
WO2009120374A3 (en) Methods and compositions for nucleic acid sample preparation
WO2009092035A3 (en) Methods and compositions for the analysis of biological molecules
WO2010107946A3 (en) Use of thermostable endonucleases for generating reporter molecules
WO2010127304A3 (en) Sequencing methods
WO2009102983A3 (en) Efficient induction of pluripotent stem cells using small molecule compounds
WO2009124255A8 (en) Methods for transcript analysis
EP2121956A4 (de) Verfahren und zusammensetzungen zur erweiterung von nukleinsäuren
WO2010048337A3 (en) Preservation of information related to genomic dna methylation
WO2007139723A8 (en) Chemicallymodified oligonucleotide primers for nucleic acid amplification
EP2224017A4 (de) Verfahren zur erhöhung der nukleinsäure-zielsequenz und dafür verwendete sonde
IL208036A0 (en) Compositions and methods for analysis of nucleic acid molecules during amplification reactions
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
EP2314680A4 (de) Verfahren zur amplifikation einer zielnukleinsäuresequenz, verfahren zum mutationsnachweis mit dem verfahren und reagentien zur verwendung in den verfahren
WO2007093050A8 (en) Gene expression assays conducted by elemental analysis
WO2009039189A3 (en) Compositions comprising stat3 sirna and methods of use thereof
EP2058396A4 (de) Nukleinsäureamplifikationsverfahren
WO2009108217A3 (en) Compositions comprising k-ras sirna and methods of use
WO2008082429A3 (en) Plant-specific genetic elements and transfer cassettes for plant transformation
WO2005110067A3 (en) Method and compositions for rna interference
WO2008079895A3 (en) Methods of obtaining genetic competence in bacillus cells
BRPI0815392A2 (pt) Método para produzir planta transgênica, planta transgênica, uso de uma sequência de ácido nucleico exógeno, gene quimérico, vetor de transformação de planta, e , planta ou célula de planta.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08768763

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08768763

Country of ref document: EP

Kind code of ref document: A2